Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50084.t1 | AFK46071.1 | 98 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 2.50E-20 | 107.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50084.t1 | P49310 | 82.9 | 35 | 6 | 0 | 3 | 37 | 5 | 39 | 2.4e-11 | 68.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50084.t1 | I3T0M9 | 98.0 | 51 | 1 | 0 | 1 | 51 | 1 | 51 | 1.8e-20 | 107.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050702 | MS.gene50084 | -0.868621 | 5.70E-66 | -1.69E-46 |
| MS.gene053401 | MS.gene50084 | 0.823901 | 1.08E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50084.t1 | MTR_4g070080 | 94.595 | 37 | 2 | 0 | 1 | 37 | 1 | 37 | 9.38e-21 | 78.2 |
| MS.gene50084.t1 | MTR_4g070080 | 94.595 | 37 | 2 | 0 | 1 | 37 | 1 | 37 | 2.11e-20 | 78.2 |
| MS.gene50084.t1 | MTR_4g070190 | 88.889 | 36 | 4 | 0 | 2 | 37 | 4 | 39 | 2.53e-18 | 73.2 |
| MS.gene50084.t1 | MTR_4g070140 | 80.556 | 36 | 7 | 0 | 2 | 37 | 4 | 39 | 1.85e-15 | 66.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50084.t1 | AT2G21660 | 75.000 | 36 | 9 | 0 | 2 | 37 | 4 | 39 | 5.58e-15 | 64.3 |
| MS.gene50084.t1 | AT2G21660 | 75.000 | 36 | 9 | 0 | 2 | 37 | 4 | 39 | 7.62e-15 | 64.3 |
| MS.gene50084.t1 | AT4G39260 | 62.162 | 37 | 14 | 0 | 1 | 37 | 1 | 37 | 2.02e-14 | 61.2 |
| MS.gene50084.t1 | AT4G39260 | 62.162 | 37 | 14 | 0 | 1 | 37 | 1 | 37 | 3.43e-14 | 60.8 |
| MS.gene50084.t1 | AT4G39260 | 62.162 | 37 | 14 | 0 | 1 | 37 | 1 | 37 | 4.44e-14 | 61.2 |
| MS.gene50084.t1 | AT4G39260 | 62.162 | 37 | 14 | 0 | 1 | 37 | 1 | 37 | 1.03e-13 | 61.2 |
Find 15 sgRNAs with CRISPR-Local
Find 15 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGGTCCGTTTGTTGCTTGC+AGG | 0.272514 | 4.2:-36768100 | MS.gene50084:CDS |
| CGTTTGTTGCTTGCAGGCTT+CGG | 0.338723 | 4.2:-36768094 | MS.gene50084:CDS |
| GCTTCGTCGGAGGTCTTGCA+TGG | 0.475042 | 4.2:-36768183 | MS.gene50084:CDS |
| TGCAAGCAACAAACGGACCT+TGG | 0.502405 | 4.2:+36768102 | None:intergenic |
| CAAGACCTCCGACGAAGCAT+CGG | 0.524569 | 4.2:+36768188 | None:intergenic |
| GTTGAATACCGATGCTTCGT+CGG | 0.525860 | 4.2:-36768196 | MS.gene50084:CDS |
| GTTGCTTGCAGGCTTCGGAT+CGG | 0.531224 | 4.2:-36768089 | MS.gene50084:CDS |
| CGAAGCCTGCAAGCAACAAA+CGG | 0.535699 | 4.2:+36768095 | None:intergenic |
| CGGCGAGATCGTTGATTCCA+AGG | 0.539852 | 4.2:-36768119 | MS.gene50084:CDS |
| GAGAAAGCCTTCTCTCAATA+CGG | 0.543855 | 4.2:-36768139 | MS.gene50084:CDS |
| TTTCTCGAGAGCATCATTGT+CGG | 0.550717 | 4.2:+36768156 | None:intergenic |
| GAATACCGATGCTTCGTCGG+AGG | 0.590495 | 4.2:-36768193 | MS.gene50084:CDS |
| CTCGAGAGCATCATTGTCGG+TGG | 0.591544 | 4.2:+36768159 | None:intergenic |
| CTTCGTCGGAGGTCTTGCAT+GGG | 0.591814 | 4.2:-36768182 | MS.gene50084:CDS |
| GATCTCGCCGTATTGAGAGA+AGG | 0.650761 | 4.2:+36768132 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| GAGAAAGCCTTCTCTCAATA+CGG | - | chr4.2:36768138-36768157 | MS.gene50084:CDS | 40.0% | |
| !! | TTTCTCGAGAGCATCATTGT+CGG | + | chr4.2:36768124-36768143 | None:intergenic | 40.0% |
| GTTGAATACCGATGCTTCGT+CGG | - | chr4.2:36768081-36768100 | MS.gene50084:CDS | 45.0% | |
| CGAAGCCTGCAAGCAACAAA+CGG | + | chr4.2:36768185-36768204 | None:intergenic | 50.0% | |
| GATCTCGCCGTATTGAGAGA+AGG | + | chr4.2:36768148-36768167 | None:intergenic | 50.0% | |
| TGCAAGCAACAAACGGACCT+TGG | + | chr4.2:36768178-36768197 | None:intergenic | 50.0% | |
| ! | AAGGTCCGTTTGTTGCTTGC+AGG | - | chr4.2:36768177-36768196 | MS.gene50084:CDS | 50.0% |
| ! | CGTTTGTTGCTTGCAGGCTT+CGG | - | chr4.2:36768183-36768202 | MS.gene50084:CDS | 50.0% |
| CAAGACCTCCGACGAAGCAT+CGG | + | chr4.2:36768092-36768111 | None:intergenic | 55.0% | |
| CTTCGTCGGAGGTCTTGCAT+GGG | - | chr4.2:36768095-36768114 | MS.gene50084:CDS | 55.0% | |
| GAATACCGATGCTTCGTCGG+AGG | - | chr4.2:36768084-36768103 | MS.gene50084:CDS | 55.0% | |
| GTTGCTTGCAGGCTTCGGAT+CGG | - | chr4.2:36768188-36768207 | MS.gene50084:CDS | 55.0% | |
| !! | CGGCGAGATCGTTGATTCCA+AGG | - | chr4.2:36768158-36768177 | MS.gene50084:CDS | 55.0% |
| !! | CTCGAGAGCATCATTGTCGG+TGG | + | chr4.2:36768121-36768140 | None:intergenic | 55.0% |
| GCTTCGTCGGAGGTCTTGCA+TGG | - | chr4.2:36768094-36768113 | MS.gene50084:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.2 | gene | 36768072 | 36768227 | 36768072 | ID=MS.gene50084 |
| chr4.2 | mRNA | 36768072 | 36768227 | 36768072 | ID=MS.gene50084.t1;Parent=MS.gene50084 |
| chr4.2 | exon | 36768072 | 36768227 | 36768072 | ID=MS.gene50084.t1.exon1;Parent=MS.gene50084.t1 |
| chr4.2 | CDS | 36768072 | 36768227 | 36768072 | ID=cds.MS.gene50084.t1;Parent=MS.gene50084.t1 |
| Gene Sequence |
| Protein sequence |
