Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052447 | MS.gene50274 | 0.81244 | 4.24E-51 | -1.69E-46 |
| MS.gene05329 | MS.gene50274 | 0.801622 | 8.26E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 26 sgRNAs with CRISPR-Local
Find 89 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATTACGTTCTTTCTCCTAA+TGG | 0.285515 | 4.4:+77389882 | MS.gene50274:CDS |
| GATTCTGTTCTTGCTGCTGC+TGG | 0.321273 | 4.4:+77390684 | MS.gene50274:CDS |
| CATGTTCAGCTTCATCAGTT+TGG | 0.328254 | 4.4:-77389935 | None:intergenic |
| CATGTTCAGCTTCATCAGTT+TGG | 0.328254 | 4.4:-77390196 | None:intergenic |
| CATGTTCAGCTTCATCAGTT+TGG | 0.328254 | 4.4:-77390463 | None:intergenic |
| GACTTCGACAGCTTCCGTTC+TGG | 0.330260 | 4.4:+77390400 | MS.gene50274:CDS |
| GTCAGTATTCATTATCTTCA+TGG | 0.348388 | 4.4:+77388527 | MS.gene50274:CDS |
| TGGACATCTCCGGCAACATT+TGG | 0.372391 | 4.4:-77389915 | None:intergenic |
| GACTTTGACAGATTCCGTTT+TGG | 0.388367 | 4.4:+77390139 | MS.gene50274:CDS |
| ATTAAAATGCCAAATGTTGC+CGG | 0.396189 | 4.4:+77389906 | MS.gene50274:CDS |
| GGACATCTCCGGCAACCTCT+TGG | 0.443204 | 4.4:-77390442 | None:intergenic |
| CAGTTTGGACATCAACCTCT+TGG | 0.447564 | 4.4:-77390181 | None:intergenic |
| CAAACGCAACCTTCGTAAAG+AGG | 0.449874 | 4.4:+77388502 | MS.gene50274:CDS |
| GCTTCCGTTCTGGCTGCTGC+TGG | 0.473589 | 4.4:+77390410 | MS.gene50274:CDS |
| ACTAAAGTCCAAGAGGTTGC+CGG | 0.481696 | 4.4:+77390434 | MS.gene50274:CDS |
| TTCATCAGTTTGGACATCTC+CGG | 0.509981 | 4.4:-77389925 | None:intergenic |
| TTCATCAGTTTGGACATCTC+CGG | 0.509981 | 4.4:-77390453 | None:intergenic |
| AGTACCAGCAGCAGCCAAAA+CGG | 0.549099 | 4.4:-77390153 | None:intergenic |
| GACACCACATTAAGCTTCAT+CGG | 0.549342 | 4.4:-77390727 | None:intergenic |
| AGTACCAGCAGCAGCCAGAA+CGG | 0.581511 | 4.4:-77390414 | None:intergenic |
| TACTGACGTCCTCTTTACGA+AGG | 0.633504 | 4.4:-77388511 | None:intergenic |
| CAAACTGATGAAGCTGAACA+TGG | 0.641263 | 4.4:+77389936 | MS.gene50274:CDS |
| CAAACTGATGAAGCTGAACA+TGG | 0.641263 | 4.4:+77390197 | MS.gene50274:CDS |
| CAAACTGATGAAGCTGAACA+TGG | 0.641263 | 4.4:+77390464 | MS.gene50274:CDS |
| TGCTGGTACTAAAGTCCAAG+AGG | 0.660328 | 4.4:+77390166 | MS.gene50274:CDS |
| TGCTGGTACTAAAGTCCAAG+AGG | 0.660328 | 4.4:+77390427 | MS.gene50274:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATAAATATGAATATGAATAT+TGG | + | chr4.4:77388412-77388431 | MS.gene50274:intron | 10.0% |
| !!! | AGTTTATGATATTTTTTTTA+TGG | - | chr4.4:77389097-77389116 | None:intergenic | 10.0% |
| !! | AAAACTAAAAACTAAAAACA+TGG | - | chr4.4:77388378-77388397 | None:intergenic | 15.0% |
| !! | AATTTATTATAGGGTAAAAA+AGG | + | chr4.4:77389345-77389364 | MS.gene50274:intron | 15.0% |
| !! | AGCTATAAATAAAGTTAAAT+TGG | - | chr4.4:77388743-77388762 | None:intergenic | 15.0% |
| !!! | CTAGACATAATTATTTTATA+GGG | + | chr4.4:77389647-77389666 | MS.gene50274:intron | 15.0% |
| !!! | GATTTTAGAAAAAATAGTAA+GGG | + | chr4.4:77389367-77389386 | MS.gene50274:intron | 15.0% |
| !!! | TCTAGACATAATTATTTTAT+AGG | + | chr4.4:77389646-77389665 | MS.gene50274:intron | 15.0% |
| !! | ATTAGTATGAAAACGAAAAT+AGG | + | chr4.4:77388902-77388921 | MS.gene50274:intron | 20.0% |
| !! | TCATAAAAGTTAACAAAACA+AGG | - | chr4.4:77388853-77388872 | None:intergenic | 20.0% |
| !! | TTAGTATGAAAACGAAAATA+GGG | + | chr4.4:77388903-77388922 | MS.gene50274:intron | 20.0% |
| !!! | AAAAAATCAGTTTCAAGTTT+AGG | + | chr4.4:77388633-77388652 | MS.gene50274:intron | 20.0% |
| !!! | AAAAATCAGTTTCAAGTTTA+GGG | + | chr4.4:77388634-77388653 | MS.gene50274:intron | 20.0% |
| !!! | GGATTTTAGAAAAAATAGTA+AGG | + | chr4.4:77389366-77389385 | MS.gene50274:intron | 20.0% |
| !!! | TCGTTTTCATACTAATTATT+TGG | - | chr4.4:77388898-77388917 | None:intergenic | 20.0% |
| ! | AAAAATAGTAAGGGTAAAAG+TGG | + | chr4.4:77389376-77389395 | MS.gene50274:intron | 25.0% |
| ! | ACAATCATTAATTTCTCACA+CGG | - | chr4.4:77390633-77390652 | None:intergenic | 25.0% |
| ! | ACTCAGATTAAAAAGCTAAT+CGG | - | chr4.4:77390292-77390311 | None:intergenic | 25.0% |
| ! | ATTGTACTTCAATTGTTTGT+TGG | - | chr4.4:77389128-77389147 | None:intergenic | 25.0% |
| ! | CTGATAAAAAAAGCTATGTT+TGG | - | chr4.4:77389551-77389570 | None:intergenic | 25.0% |
| ! | TGAAATAAGCTATAAACTCA+TGG | + | chr4.4:77389512-77389531 | MS.gene50274:intron | 25.0% |
| !! | AATTAGCTTATAGCTTATAG+CGG | - | chr4.4:77389591-77389610 | None:intergenic | 25.0% |
| !! | AGTTATATCATTGATTGTTC+TGG | + | chr4.4:77389262-77389281 | MS.gene50274:intron | 25.0% |
| !! | ATTATTGTGTTTGAAATTGC+AGG | + | chr4.4:77390110-77390129 | MS.gene50274:intron | 25.0% |
| !! | ATTTCTGATTTGATTGTGTT+TGG | - | chr4.4:77389018-77389037 | None:intergenic | 25.0% |
| !! | GATTGTGTAACTTTTATTCA+AGG | + | chr4.4:77388330-77388349 | MS.gene50274:CDS | 25.0% |
| !! | TTGTGTTTGGATTTTATTGA+TGG | - | chr4.4:77389005-77389024 | None:intergenic | 25.0% |
| !!! | ATAATCGTTATTTTCTCACA+TGG | - | chr4.4:77390095-77390114 | None:intergenic | 25.0% |
| !!! | TTTGGCATTTTAATTCCATT+AGG | - | chr4.4:77389900-77389919 | None:intergenic | 25.0% |
| ACTCTGATTGGTAAAAATAG+CGG | + | chr4.4:77388951-77388970 | MS.gene50274:intron | 30.0% | |
| ATGGAAATCGTTGTGAAAAA+GGG | - | chr4.4:77389078-77389097 | None:intergenic | 30.0% | |
| ATTAAAATGCCAAATGTTGC+CGG | + | chr4.4:77389906-77389925 | MS.gene50274:CDS | 30.0% | |
| ATTATCTTCATGGTATGCTT+TGG | + | chr4.4:77388537-77388556 | MS.gene50274:intron | 30.0% | |
| CAGTTTATTTAGACTCTGAT+TGG | + | chr4.4:77388939-77388958 | MS.gene50274:intron | 30.0% | |
| GTCAGTATTCATTATCTTCA+TGG | + | chr4.4:77388527-77388546 | MS.gene50274:CDS | 30.0% | |
| TAACAAAACAAGGACTAACA+TGG | - | chr4.4:77388843-77388862 | None:intergenic | 30.0% | |
| TATGGAAATCGTTGTGAAAA+AGG | - | chr4.4:77389079-77389098 | None:intergenic | 30.0% | |
| TCAGAAATGAGAATGATACT+TGG | + | chr4.4:77389030-77389049 | MS.gene50274:intron | 30.0% | |
| TTCAAGAGAAATGAAAACCT+GGG | + | chr4.4:77389168-77389187 | MS.gene50274:intron | 30.0% | |
| ! | ATGATTGTGTTTGAAATTGC+AGG | + | chr4.4:77390645-77390664 | MS.gene50274:intron | 30.0% |
| ! | TCAAGCTTTTTATCCAAACA+AGG | + | chr4.4:77388810-77388829 | MS.gene50274:intron | 30.0% |
| !! | ATAAGTTTTGTGAGACTGTT+TGG | + | chr4.4:77388699-77388718 | MS.gene50274:intron | 30.0% |
| !! | TAAGTTTTGTGAGACTGTTT+GGG | + | chr4.4:77388700-77388719 | MS.gene50274:intron | 30.0% |
| !!! | CATTTGCAAAAGTGTTTTTC+CGG | - | chr4.4:77390240-77390259 | None:intergenic | 30.0% |
| ACATGGATACAAACCTTGTT+TGG | - | chr4.4:77388826-77388845 | None:intergenic | 35.0% | |
| CATTTGCAAAAGAGAGTTTC+CGG | - | chr4.4:77389979-77389998 | None:intergenic | 35.0% | |
| CTATAAGCTCAAACACTACT+TGG | + | chr4.4:77389415-77389434 | MS.gene50274:intron | 35.0% | |
| GATTACGTTCTTTCTCCTAA+TGG | + | chr4.4:77389882-77389901 | MS.gene50274:CDS | 35.0% | |
| GTTCAAGAGAAATGAAAACC+TGG | + | chr4.4:77389167-77389186 | MS.gene50274:intron | 35.0% | |
| TCTTACCAAACAGACCTTTA+GGG | + | chr4.4:77389617-77389636 | MS.gene50274:intron | 35.0% | |
| TTGTGTGTTTATATCTGCAG+CGG | + | chr4.4:77388441-77388460 | MS.gene50274:intron | 35.0% | |
| TTTAACCCTAAAGGTCTGTT+TGG | - | chr4.4:77389625-77389644 | None:intergenic | 35.0% | |
| ! | TGTCTAGAGTTTAACCCTAA+AGG | - | chr4.4:77389634-77389653 | None:intergenic | 35.0% |
| !! | TCATTGATTGTTCTGGAGAA+TGG | + | chr4.4:77389269-77389288 | MS.gene50274:intron | 35.0% |
| !! | TTTCAAGTTTAGGGTCTGTT+TGG | + | chr4.4:77388643-77388662 | MS.gene50274:intron | 35.0% |
| CAAACTGATGAAGCTGAACA+TGG | + | chr4.4:77389936-77389955 | MS.gene50274:CDS | 40.0% | |
| CATGTTCAGCTTCATCAGTT+TGG | - | chr4.4:77389938-77389957 | None:intergenic | 40.0% | |
| CTATAAGCTCAAACGCTACT+TGG | + | chr4.4:77389458-77389477 | MS.gene50274:intron | 40.0% | |
| GGTATCAGTATTAAGTCCAC+CGG | + | chr4.4:77390485-77390504 | MS.gene50274:intron | 40.0% | |
| GGTATCTCTATTAAGTCCAC+CGG | + | chr4.4:77390218-77390237 | MS.gene50274:intron | 40.0% | |
| GTCTTACCAAACAGACCTTT+AGG | + | chr4.4:77389616-77389635 | MS.gene50274:intron | 40.0% | |
| GTGTAAACGAATCAGTGGAA+CGG | + | chr4.4:77389192-77389211 | MS.gene50274:intron | 40.0% | |
| TTCATCAGTTTGGACATCTC+CGG | - | chr4.4:77389928-77389947 | None:intergenic | 40.0% | |
| ! | GACTTTGACAGATTCCGTTT+TGG | + | chr4.4:77390139-77390158 | MS.gene50274:CDS | 40.0% |
| ! | GTTTAGGGTCTGTTTGGATT+AGG | + | chr4.4:77388649-77388668 | MS.gene50274:intron | 40.0% |
| !!! | TTGCAAAAGTGTTTTTCCGG+TGG | - | chr4.4:77390237-77390256 | None:intergenic | 40.0% |
| ACTAAAGTCCAAGAGGTTGC+CGG | + | chr4.4:77390434-77390453 | MS.gene50274:CDS | 45.0% | |
| AGCAGGAATCAGAGTTGTGA+AGG | + | chr4.4:77389227-77389246 | MS.gene50274:intron | 45.0% | |
| CAAACGCAACCTTCGTAAAG+AGG | + | chr4.4:77388502-77388521 | MS.gene50274:CDS | 45.0% | |
| CAGTTTGGACATCAACCTCT+TGG | - | chr4.4:77390184-77390203 | None:intergenic | 45.0% | |
| GGTATCACTACTAAGTCCAC+CGG | + | chr4.4:77389957-77389976 | MS.gene50274:intron | 45.0% | |
| TAAACGAATCAGTGGAACGG+CGG | + | chr4.4:77389195-77389214 | MS.gene50274:intron | 45.0% | |
| TACTGACGTCCTCTTTACGA+AGG | - | chr4.4:77388514-77388533 | None:intergenic | 45.0% | |
| TCACAACTCTGATTCCTGCT+CGG | - | chr4.4:77389227-77389246 | None:intergenic | 45.0% | |
| TGCTGGTACTAAAGTCCAAG+AGG | + | chr4.4:77390166-77390185 | MS.gene50274:CDS | 45.0% | |
| TGGGTGTGTAAACGAATCAG+TGG | + | chr4.4:77389187-77389206 | MS.gene50274:intron | 45.0% | |
| TTGCAAAAGAGAGTTTCCGG+TGG | - | chr4.4:77389976-77389995 | None:intergenic | 45.0% | |
| !! | ACTGATTCGTTTACACACCC+AGG | - | chr4.4:77389188-77389207 | None:intergenic | 45.0% |
| !!! | ATCATTATTTAATTTATTAT+AGG | + | chr4.4:77389335-77389354 | MS.gene50274:intron | 5.0% |
| !!! | TCATTATTTAATTTATTATA+GGG | + | chr4.4:77389336-77389355 | MS.gene50274:intron | 5.0% |
| AGTACCAGCAGCAGCCAAAA+CGG | - | chr4.4:77390156-77390175 | None:intergenic | 50.0% | |
| GATTCTGTTCTTGCTGCTGC+TGG | + | chr4.4:77390684-77390703 | MS.gene50274:CDS | 50.0% | |
| TGGACATCTCCGGCAACATT+TGG | - | chr4.4:77389918-77389937 | None:intergenic | 50.0% | |
| AGTACCAGCAGCAGCCAGAA+CGG | - | chr4.4:77390417-77390436 | None:intergenic | 55.0% | |
| GACTTCGACAGCTTCCGTTC+TGG | + | chr4.4:77390400-77390419 | MS.gene50274:CDS | 55.0% | |
| !!! | GATTCCGTTTTGGCTGCTGC+TGG | + | chr4.4:77390149-77390168 | MS.gene50274:CDS | 55.0% |
| GGACATCTCCGGCAACCTCT+TGG | - | chr4.4:77390445-77390464 | None:intergenic | 60.0% | |
| ! | GCTTCCGTTCTGGCTGCTGC+TGG | + | chr4.4:77390410-77390429 | MS.gene50274:CDS | 65.0% |
| AACGGCGGACGAGTCCGAGC+AGG | + | chr4.4:77389210-77389229 | MS.gene50274:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.4 | gene | 77388327 | 77390740 | 77388327 | ID=MS.gene50274 |
| chr4.4 | mRNA | 77388327 | 77390740 | 77388327 | ID=MS.gene50274.t1;Parent=MS.gene50274 |
| chr4.4 | exon | 77388327 | 77388362 | 77388327 | ID=MS.gene50274.t1.exon1;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77388327 | 77388362 | 77388327 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| chr4.4 | exon | 77388461 | 77388548 | 77388461 | ID=MS.gene50274.t1.exon2;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77388461 | 77388548 | 77388461 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| chr4.4 | exon | 77389862 | 77389957 | 77389862 | ID=MS.gene50274.t1.exon3;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77389862 | 77389957 | 77389862 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| chr4.4 | exon | 77390132 | 77390218 | 77390132 | ID=MS.gene50274.t1.exon4;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77390132 | 77390218 | 77390132 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| chr4.4 | exon | 77390393 | 77390485 | 77390393 | ID=MS.gene50274.t1.exon5;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77390393 | 77390485 | 77390393 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| chr4.4 | exon | 77390667 | 77390740 | 77390667 | ID=MS.gene50274.t1.exon6;Parent=MS.gene50274.t1 |
| chr4.4 | CDS | 77390667 | 77390740 | 77390667 | ID=cds.MS.gene50274.t1;Parent=MS.gene50274.t1 |
| Gene Sequence |
| Protein sequence |
