Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50520.t1 | XP_013449696.1 | 100 | 198 | 0 | 0 | 1 | 198 | 208 | 405 | 1.60E-107 | 398.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50520.t1 | Q9M8J9 | 73.2 | 198 | 53 | 0 | 1 | 198 | 203 | 400 | 7.6e-82 | 304.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50520.t1 | A0A072U1Y3 | 100.0 | 198 | 0 | 0 | 1 | 198 | 208 | 405 | 1.2e-107 | 398.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057148 | MS.gene50520 | -0.816604 | 5.08E-52 | -1.69E-46 |
| MS.gene059778 | MS.gene50520 | 0.807031 | 6.16E-50 | -1.69E-46 |
| MS.gene060338 | MS.gene50520 | 0.814427 | 1.55E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 44 sgRNAs with CRISPR-Local
Find 99 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTGATGGTAGCGACATAAA+TGG | 0.313292 | 7.4:-14731341 | MS.gene50520:CDS |
| TTACTGGGATGAACACAAAA+TGG | 0.317610 | 7.4:-14732341 | MS.gene50520:CDS |
| AGTTGTAGCCACGGTATTAA+TGG | 0.325761 | 7.4:+14732218 | None:intergenic |
| CCTGACATGCAAGAGCTTAC+TGG | 0.346812 | 7.4:-14732357 | MS.gene50520:CDS |
| CCAGTAAGCTCTTGCATGTC+AGG | 0.381423 | 7.4:+14732357 | None:intergenic |
| ATCTGTTCTGACTGAGCTAC+TGG | 0.388739 | 7.4:-14731426 | MS.gene50520:CDS |
| TGTCTTCAACTTTAATAGAC+TGG | 0.397130 | 7.4:+14732707 | None:intergenic |
| AAAATGGGCAACAACCTTGA+TGG | 0.400210 | 7.4:-14732325 | MS.gene50520:CDS |
| AAAAGCTGGGCTCTGAAAAC+AGG | 0.418885 | 7.4:+14732754 | None:intergenic |
| GGCTAGATTTACTGAGCATT+CGG | 0.429590 | 7.4:-14731396 | MS.gene50520:CDS |
| TATAAGCCTGAGCCTCTCTA+AGG | 0.439005 | 7.4:+14732269 | None:intergenic |
| TGATGGATTTGAGTACTTGA+AGG | 0.465111 | 7.4:-14731459 | MS.gene50520:CDS |
| CTGACATGCAAGAGCTTACT+GGG | 0.474237 | 7.4:-14732356 | MS.gene50520:CDS |
| AGCAGCCGCTAGAAGATGTT+GGG | 0.482623 | 7.4:+14732302 | None:intergenic |
| TTGATGGTAGCGACATAAAT+GGG | 0.492627 | 7.4:-14731340 | MS.gene50520:CDS |
| TGAGACAGACTGCTTTCAGC+TGG | 0.493739 | 7.4:+14732166 | None:intergenic |
| CGTGGCTACAACTTTAGCCT+TGG | 0.515788 | 7.4:-14732208 | MS.gene50520:CDS |
| GTTATGCCTTAGAGAGGCTC+AGG | 0.515843 | 7.4:-14732275 | MS.gene50520:CDS |
| AGTAGCTCAGTCAGAACAGA+TGG | 0.522563 | 7.4:+14731428 | None:intergenic |
| GTCTTCAACTTTAATAGACT+GGG | 0.522594 | 7.4:+14732708 | None:intergenic |
| AGGAATGAAGCAATACTTGA+TGG | 0.534209 | 7.4:-14731356 | MS.gene50520:CDS |
| TCAGCTGTGATGCAAACTGA+TGG | 0.535341 | 7.4:-14731476 | MS.gene50520:intron |
| CAGCAGCCGCTAGAAGATGT+TGG | 0.535413 | 7.4:+14732301 | None:intergenic |
| TACTGGGATGAACACAAAAT+GGG | 0.538886 | 7.4:-14732340 | MS.gene50520:CDS |
| ATGTGAGCTTTGAAGTGGAT+GGG | 0.539335 | 7.4:-14732819 | MS.gene50520:CDS |
| TAGAAGATGTTGGGCCATCA+AGG | 0.550820 | 7.4:+14732311 | None:intergenic |
| TGGGTACTTTGATCTCTCAT+AGG | 0.553110 | 7.4:+14732727 | None:intergenic |
| TAGCGACATAAATGGGAGGC+GGG | 0.581746 | 7.4:-14731333 | None:intergenic |
| ACAGTGGTGTTGCTCTGCCA+AGG | 0.585829 | 7.4:+14732191 | None:intergenic |
| GTCTATTAAAGTTGAAGACA+TGG | 0.587011 | 7.4:-14732704 | MS.gene50520:CDS |
| GTAGCGACATAAATGGGAGG+CGG | 0.587515 | 7.4:-14731334 | MS.gene50520:CDS |
| TGTGAGCTTTGAAGTGGATG+GGG | 0.599589 | 7.4:-14732818 | MS.gene50520:CDS |
| CTGCTTTCAGCTGGAAACAG+TGG | 0.607188 | 7.4:+14732175 | None:intergenic |
| GATGTGAGCTTTGAAGTGGA+TGG | 0.611734 | 7.4:-14732820 | MS.gene50520:CDS |
| CAAGGCTAAAGTTGTAGCCA+CGG | 0.622185 | 7.4:+14732209 | None:intergenic |
| AAGTGATGTGAGCTTTGAAG+TGG | 0.629597 | 7.4:-14732824 | MS.gene50520:CDS |
| CTGATCGTTATGCCTTAGAG+AGG | 0.645940 | 7.4:-14732281 | MS.gene50520:CDS |
| GTAGCTCAGTCAGAACAGAT+GGG | 0.683418 | 7.4:+14731429 | None:intergenic |
| GACTGAGCTACTGGAGTACG+TGG | 0.688714 | 7.4:-14731417 | MS.gene50520:CDS |
| GATGGCCCAACATCTTCTAG+CGG | 0.704220 | 7.4:-14732307 | MS.gene50520:CDS |
| GCCACCTCTGAAAATCTCAG+AGG | 0.704264 | 7.4:-14732135 | MS.gene50520:intron |
| ATGGTAGCGACATAAATGGG+AGG | 0.709241 | 7.4:-14731337 | MS.gene50520:CDS |
| TATTAAAGTTGAAGACATGG+AGG | 0.710382 | 7.4:-14732701 | MS.gene50520:CDS |
| AGATGTCGCCATTAATACCG+TGG | 0.713502 | 7.4:-14732226 | MS.gene50520:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AACTAATTAACTTTGAATAA+TGG | + | chr7.4:14732165-14732184 | None:intergenic | 15.0% |
| !! | TTAAATAAAACCTTAAAAAC+TGG | + | chr7.4:14731482-14731501 | None:intergenic | 15.0% |
| !! | TTAATATCTTAAATATGTAC+AGG | - | chr7.4:14731510-14731529 | MS.gene50520:intron | 15.0% |
| !!! | CTGAACTATTTTTATTATTT+TGG | - | chr7.4:14732652-14732671 | MS.gene50520:intron | 15.0% |
| !! | AAATTAGTAGTAAATGGAAA+AGG | + | chr7.4:14732300-14732319 | None:intergenic | 20.0% |
| !! | ATAATTACACAACAACTATT+TGG | - | chr7.4:14731617-14731636 | MS.gene50520:intron | 20.0% |
| !! | ATGAAGAATAAAAAAAGGTT+GGG | + | chr7.4:14732613-14732632 | None:intergenic | 20.0% |
| !! | GTTGTGTAATTATTTACAAA+AGG | + | chr7.4:14731610-14731629 | None:intergenic | 20.0% |
| ! | AAGATAAAAGGAACAATGAA+AGG | + | chr7.4:14731738-14731757 | None:intergenic | 25.0% |
| ! | ATAAATTTCTAATGGAATCG+TGG | + | chr7.4:14732559-14732578 | None:intergenic | 25.0% |
| ! | ATCATAAAAAGAACCATCTT+CGG | + | chr7.4:14731559-14731578 | None:intergenic | 25.0% |
| ! | ATTTACAAAAGGCAAAACAA+TGG | + | chr7.4:14731599-14731618 | None:intergenic | 25.0% |
| ! | CTTGGATGAAGAATAAAAAA+AGG | + | chr7.4:14732618-14732637 | None:intergenic | 25.0% |
| ! | GATGAAGAATAAAAAAAGGT+TGG | + | chr7.4:14732614-14732633 | None:intergenic | 25.0% |
| ! | GATTCCATTAGAAATTTATC+TGG | - | chr7.4:14732560-14732579 | MS.gene50520:intron | 25.0% |
| ! | TCAGTAGATATAAATGTTCT+TGG | + | chr7.4:14732636-14732655 | None:intergenic | 25.0% |
| ! | TTAATCATAATATCCGAAGA+TGG | - | chr7.4:14731543-14731562 | MS.gene50520:intron | 25.0% |
| !! | ATTGCTTCATTTTATGTACT+GGG | - | chr7.4:14731767-14731786 | MS.gene50520:intron | 25.0% |
| !! | GTTCTTTTTATGATTTGATG+AGG | - | chr7.4:14731565-14731584 | MS.gene50520:intron | 25.0% |
| !! | TAAGCTTTGTATGATTCATA+TGG | - | chr7.4:14732256-14732275 | MS.gene50520:CDS | 25.0% |
| ATAGATGTTTCAACATATCC+TGG | + | chr7.4:14732494-14732513 | None:intergenic | 30.0% | |
| CAGGACAAATTAGTAGTAAA+TGG | + | chr7.4:14732306-14732325 | None:intergenic | 30.0% | |
| CTAATTGCATAAGCTATTGA+TGG | - | chr7.4:14732527-14732546 | MS.gene50520:intron | 30.0% | |
| CTTTCAGAATTTAGACCTTT+CGG | + | chr7.4:14732124-14732143 | None:intergenic | 30.0% | |
| GTCTATTAAAGTTGAAGACA+TGG | - | chr7.4:14731451-14731470 | MS.gene50520:CDS | 30.0% | |
| GTCTTCAACTTTAATAGACT+GGG | + | chr7.4:14731450-14731469 | None:intergenic | 30.0% | |
| TAGGCCAGATAAATTTCTAA+TGG | + | chr7.4:14732567-14732586 | None:intergenic | 30.0% | |
| TATTAAAGTTGAAGACATGG+AGG | - | chr7.4:14731454-14731473 | MS.gene50520:CDS | 30.0% | |
| TCAACAAATTAGCTCAAGTT+AGG | + | chr7.4:14732586-14732605 | None:intergenic | 30.0% | |
| TGTCTTCAACTTTAATAGAC+TGG | + | chr7.4:14731451-14731470 | None:intergenic | 30.0% | |
| ! | AGTTTTCACTGCTCATAAAT+TGG | - | chr7.4:14731361-14731380 | MS.gene50520:CDS | 30.0% |
| ! | CATTGCTTCATTTTATGTAC+TGG | - | chr7.4:14731766-14731785 | MS.gene50520:intron | 30.0% |
| ! | CTTTTTATGATTTGATGAGG+AGG | - | chr7.4:14731568-14731587 | MS.gene50520:intron | 30.0% |
| !! | TCTGAAAGTTGATATCAACT+CGG | - | chr7.4:14732136-14732155 | MS.gene50520:CDS | 30.0% |
| !!! | AAAGTACAAGAGTATTTTGC+TGG | + | chr7.4:14732388-14732407 | None:intergenic | 30.0% |
| AATGCCTGCAACAAGATAAA+AGG | + | chr7.4:14731750-14731769 | None:intergenic | 35.0% | |
| AATTAGTATGTCGATCTTCC+AGG | - | chr7.4:14732473-14732492 | MS.gene50520:intron | 35.0% | |
| ACTGGTATACACAAGACTTT+AGG | + | chr7.4:14732356-14732375 | None:intergenic | 35.0% | |
| ACTTAATCGCTCACTGATAA+AGG | - | chr7.4:14732070-14732089 | MS.gene50520:intron | 35.0% | |
| ACTTGTGTTGAATGAGAATG+AGG | - | chr7.4:14731665-14731684 | MS.gene50520:intron | 35.0% | |
| AGGAATGAAGCAATACTTGA+TGG | - | chr7.4:14732799-14732818 | MS.gene50520:CDS | 35.0% | |
| ATTGAGCATGATAACTATGC+AGG | - | chr7.4:14731692-14731711 | MS.gene50520:intron | 35.0% | |
| CTGGTATACACAAGACTTTA+GGG | + | chr7.4:14732355-14732374 | None:intergenic | 35.0% | |
| TACTGGGATGAACACAAAAT+GGG | - | chr7.4:14731815-14731834 | MS.gene50520:intron | 35.0% | |
| TCTCACAACTTGCAATTGTA+TGG | + | chr7.4:14731646-14731665 | None:intergenic | 35.0% | |
| TGAGTAAAATTGACACCGAA+AGG | - | chr7.4:14732106-14732125 | MS.gene50520:intron | 35.0% | |
| TTACTGGGATGAACACAAAA+TGG | - | chr7.4:14731814-14731833 | MS.gene50520:intron | 35.0% | |
| TTGAGCATGATAACTATGCA+GGG | - | chr7.4:14731693-14731712 | MS.gene50520:intron | 35.0% | |
| ! | TGTTCCTTTTATCTTGTTGC+AGG | - | chr7.4:14731743-14731762 | MS.gene50520:intron | 35.0% |
| ! | TTGATGGTAGCGACATAAAT+GGG | - | chr7.4:14732815-14732834 | MS.gene50520:CDS | 35.0% |
| ! | TTTTGCTGGTACAAATCAAC+TGG | + | chr7.4:14732374-14732393 | None:intergenic | 35.0% |
| !! | ACAAGACTTTAGGGTAAACT+TGG | + | chr7.4:14732346-14732365 | None:intergenic | 35.0% |
| !! | TGATGGATTTGAGTACTTGA+AGG | - | chr7.4:14732696-14732715 | MS.gene50520:CDS | 35.0% |
| AAAATGGGCAACAACCTTGA+TGG | - | chr7.4:14731830-14731849 | MS.gene50520:intron | 40.0% | |
| AGTTGTAGCCACGGTATTAA+TGG | + | chr7.4:14731940-14731959 | None:intergenic | 40.0% | |
| GGCTAGATTTACTGAGCATT+CGG | - | chr7.4:14732759-14732778 | MS.gene50520:CDS | 40.0% | |
| TCTCATAGGACCAAAAAGCT+GGG | + | chr7.4:14731417-14731436 | None:intergenic | 40.0% | |
| TGGGTACTTTGATCTCTCAT+AGG | + | chr7.4:14731431-14731450 | None:intergenic | 40.0% | |
| TTCATTCAGCAAGTCCAAAC+TGG | + | chr7.4:14732229-14732248 | None:intergenic | 40.0% | |
| ! | CTTGATGGTAGCGACATAAA+TGG | - | chr7.4:14732814-14732833 | MS.gene50520:CDS | 40.0% |
| !! | ATGTGAGCTTTGAAGTGGAT+GGG | - | chr7.4:14731336-14731355 | MS.gene50520:CDS | 40.0% |
| !!! | CATACCTCTGAGATTTTCAG+AGG | + | chr7.4:14732027-14732046 | None:intergenic | 40.0% |
| AGATGTCGCCATTAATACCG+TGG | - | chr7.4:14731929-14731948 | MS.gene50520:intron | 45.0% | |
| AGTAGCTCAGTCAGAACAGA+TGG | + | chr7.4:14732730-14732749 | None:intergenic | 45.0% | |
| ATCTGTTCTGACTGAGCTAC+TGG | - | chr7.4:14732729-14732748 | MS.gene50520:CDS | 45.0% | |
| ATTCAGCAAGTCCAAACTGG+AGG | + | chr7.4:14732226-14732245 | None:intergenic | 45.0% | |
| CAAGGCTAAAGTTGTAGCCA+CGG | + | chr7.4:14731949-14731968 | None:intergenic | 45.0% | |
| CTCTCATAGGACCAAAAAGC+TGG | + | chr7.4:14731418-14731437 | None:intergenic | 45.0% | |
| CTGACATGCAAGAGCTTACT+GGG | - | chr7.4:14731799-14731818 | MS.gene50520:intron | 45.0% | |
| CTGATCGTTATGCCTTAGAG+AGG | - | chr7.4:14731874-14731893 | MS.gene50520:intron | 45.0% | |
| GGAACAATCACTGAACTTGC+AGG | + | chr7.4:14732325-14732344 | None:intergenic | 45.0% | |
| GTAGCTCAGTCAGAACAGAT+GGG | + | chr7.4:14732729-14732748 | None:intergenic | 45.0% | |
| TAGAAGATGTTGGGCCATCA+AGG | + | chr7.4:14731847-14731866 | None:intergenic | 45.0% | |
| TATAAGCCTGAGCCTCTCTA+AGG | + | chr7.4:14731889-14731908 | None:intergenic | 45.0% | |
| TCAGCTGTGATGCAAACTGA+TGG | - | chr7.4:14732679-14732698 | MS.gene50520:intron | 45.0% | |
| ! | ATGGTAGCGACATAAATGGG+AGG | - | chr7.4:14732818-14732837 | MS.gene50520:CDS | 45.0% |
| ! | CATGGAGGCTCCAGTTTTTA+AGG | - | chr7.4:14731469-14731488 | MS.gene50520:CDS | 45.0% |
| ! | CGGACTTTTTATGCAAGCAC+AGG | - | chr7.4:14732779-14732798 | MS.gene50520:CDS | 45.0% |
| ! | GTTTTCAGAGCCCAGCTTTT+TGG | - | chr7.4:14731404-14731423 | MS.gene50520:CDS | 45.0% |
| !! | AAAAGCTGGGCTCTGAAAAC+AGG | + | chr7.4:14731404-14731423 | None:intergenic | 45.0% |
| !! | GATGTGAGCTTTGAAGTGGA+TGG | - | chr7.4:14731335-14731354 | MS.gene50520:CDS | 45.0% |
| !! | TGTGAGCTTTGAAGTGGATG+GGG | - | chr7.4:14731337-14731356 | MS.gene50520:CDS | 45.0% |
| !!! | ACCTCTGAGATTTTCAGAGG+TGG | + | chr7.4:14732024-14732043 | None:intergenic | 45.0% |
| AGCAGCCGCTAGAAGATGTT+GGG | + | chr7.4:14731856-14731875 | None:intergenic | 50.0% | |
| CACTGCTATAGCCTCCAGTT+TGG | - | chr7.4:14732212-14732231 | MS.gene50520:CDS | 50.0% | |
| CCAGTAAGCTCTTGCATGTC+AGG | + | chr7.4:14731801-14731820 | None:intergenic | 50.0% | |
| CCTGACATGCAAGAGCTTAC+TGG | - | chr7.4:14731798-14731817 | MS.gene50520:intron | 50.0% | |
| CGTGGCTACAACTTTAGCCT+TGG | - | chr7.4:14731947-14731966 | MS.gene50520:intron | 50.0% | |
| CTGCTTTCAGCTGGAAACAG+TGG | + | chr7.4:14731983-14732002 | None:intergenic | 50.0% | |
| GATGGCCCAACATCTTCTAG+CGG | - | chr7.4:14731848-14731867 | MS.gene50520:intron | 50.0% | |
| GTAGCGACATAAATGGGAGG+CGG | - | chr7.4:14732821-14732840 | MS.gene50520:CDS | 50.0% | |
| GTTATGCCTTAGAGAGGCTC+AGG | - | chr7.4:14731880-14731899 | MS.gene50520:intron | 50.0% | |
| TAGCAGTGACAGGCTGACAA+AGG | + | chr7.4:14732200-14732219 | None:intergenic | 50.0% | |
| TGAGACAGACTGCTTTCAGC+TGG | + | chr7.4:14731992-14732011 | None:intergenic | 50.0% | |
| ! | GCCACCTCTGAAAATCTCAG+AGG | - | chr7.4:14732020-14732039 | MS.gene50520:intron | 50.0% |
| CAGCAGCCGCTAGAAGATGT+TGG | + | chr7.4:14731857-14731876 | None:intergenic | 55.0% | |
| CTGGAGGCTATAGCAGTGAC+AGG | + | chr7.4:14732210-14732229 | None:intergenic | 55.0% | |
| GACTGAGCTACTGGAGTACG+TGG | - | chr7.4:14732738-14732757 | MS.gene50520:CDS | 55.0% | |
| !! | ACAGTGGTGTTGCTCTGCCA+AGG | + | chr7.4:14731967-14731986 | None:intergenic | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 14731334 | 14732843 | 14731334 | ID=MS.gene50520 |
| chr7.4 | mRNA | 14731334 | 14732843 | 14731334 | ID=MS.gene50520.t1;Parent=MS.gene50520 |
| chr7.4 | exon | 14732687 | 14732843 | 14732687 | ID=MS.gene50520.t1.exon1;Parent=MS.gene50520.t1 |
| chr7.4 | CDS | 14732687 | 14732843 | 14732687 | ID=cds.MS.gene50520.t1;Parent=MS.gene50520.t1 |
| chr7.4 | exon | 14732136 | 14732412 | 14732136 | ID=MS.gene50520.t1.exon2;Parent=MS.gene50520.t1 |
| chr7.4 | CDS | 14732136 | 14732412 | 14732136 | ID=cds.MS.gene50520.t1;Parent=MS.gene50520.t1 |
| chr7.4 | exon | 14731334 | 14731494 | 14731334 | ID=MS.gene50520.t1.exon3;Parent=MS.gene50520.t1 |
| chr7.4 | CDS | 14731334 | 14731494 | 14731334 | ID=cds.MS.gene50520.t1;Parent=MS.gene50520.t1 |
| Gene Sequence |
| Protein sequence |
