Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50924.t1 | XP_003598896.1 | 96.9 | 98 | 3 | 0 | 1 | 98 | 72 | 169 | 1.30E-46 | 195.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene50924.t1 | B7FH52 | 96.9 | 98 | 3 | 0 | 1 | 98 | 72 | 169 | 9.7e-47 | 195.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057989 | MS.gene50924 | 0.844664 | 6.57E-59 | -1.69E-46 |
| MS.gene057992 | MS.gene50924 | 0.85783 | 1.25E-62 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 24 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAATGAGTTTGAGAAAT+TGG | 0.215472 | 3.3:+35027334 | MS.gene50924:CDS |
| GACTTTGGACCCCTTTCCTT+TGG | 0.317537 | 3.3:-35028171 | None:intergenic |
| GCTGGCTTCTCAAAGTGCTT+TGG | 0.365521 | 3.3:-35028207 | None:intergenic |
| AAGTGCTTTGGAGATCCAAT+AGG | 0.400879 | 3.3:-35028195 | None:intergenic |
| ATTCAATGCTTACCATGAAC+AGG | 0.405358 | 3.3:-35027416 | None:intergenic |
| CGAGGTTGGTGAATTCTTTG+AGG | 0.416409 | 3.3:-35028255 | None:intergenic |
| GGAGATCCAATAGGGGACTT+TGG | 0.419975 | 3.3:-35028186 | None:intergenic |
| TCAACAGGATCATCCATAAC+TGG | 0.427432 | 3.3:-35027383 | None:intergenic |
| AGGGGTCCAAAGTCCCCTAT+TGG | 0.433062 | 3.3:+35028180 | MS.gene50924:CDS |
| AGTGCTTTGGAGATCCAATA+GGG | 0.437450 | 3.3:-35028194 | None:intergenic |
| AAGAATTCACCAACCTCGTT+AGG | 0.440467 | 3.3:+35028260 | MS.gene50924:CDS |
| AGCTCAAGAGTGTCCAGTTA+TGG | 0.459111 | 3.3:+35027370 | MS.gene50924:CDS |
| GATCTTGTCACCAAAGGAAA+GGG | 0.460246 | 3.3:+35028161 | MS.gene50924:CDS |
| TGGACACTCTTGAGCTTCAT+CGG | 0.502009 | 3.3:-35027363 | None:intergenic |
| TTCTTTGAGGTGCACACTTG+AGG | 0.519384 | 3.3:-35028242 | None:intergenic |
| TTGAGGTTAACATATTGTGC+TGG | 0.533520 | 3.3:-35028225 | None:intergenic |
| AAAGTTTATCCTAACGAGGT+TGG | 0.543754 | 3.3:-35028269 | None:intergenic |
| TTCTTAATCAGGTGTTAACA+AGG | 0.557343 | 3.3:+35028119 | MS.gene50924:intron |
| AGATCTTGTCACCAAAGGAA+AGG | 0.561565 | 3.3:+35028160 | MS.gene50924:CDS |
| CATCAAGATCTTGTCACCAA+AGG | 0.566700 | 3.3:+35028155 | MS.gene50924:CDS |
| CTTCAAAGTTTATCCTAACG+AGG | 0.589028 | 3.3:-35028273 | None:intergenic |
| AGTTATGGATGATCCTGTTG+AGG | 0.618736 | 3.3:+35027385 | MS.gene50924:CDS |
| ATCTTGTCACCAAAGGAAAG+GGG | 0.652126 | 3.3:+35028162 | MS.gene50924:CDS |
| GTGCTTTGGAGATCCAATAG+GGG | 0.692880 | 3.3:-35028193 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATGATCAAATATAAA+CGG | - | chr3.3:35027470-35027489 | None:intergenic | 10.0% |
| !! | TAGTTTAAAAATCATACAAA+GGG | + | chr3.3:35027787-35027806 | MS.gene50924:intron | 15.0% |
| !!! | AATACTTTTTCAAACTAAAT+AGG | + | chr3.3:35027686-35027705 | MS.gene50924:intron | 15.0% |
| !!! | ATTATATTTACTCTAACTTT+TGG | - | chr3.3:35027974-35027993 | None:intergenic | 15.0% |
| !! | CTAGTTTAAAAATCATACAA+AGG | + | chr3.3:35027786-35027805 | MS.gene50924:intron | 20.0% |
| !!! | AATTGATTTCTTTCTTAATC+AGG | + | chr3.3:35028108-35028127 | MS.gene50924:intron | 20.0% |
| !!! | AGATCAATTTTATACATTGT+CGG | - | chr3.3:35027856-35027875 | None:intergenic | 20.0% |
| ! | AGATAATGAGTTTGAGAAAT+TGG | + | chr3.3:35027334-35027353 | MS.gene50924:CDS | 25.0% |
| !! | GAATCGAACTCAAAATTTTA+GGG | - | chr3.3:35028058-35028077 | None:intergenic | 25.0% |
| !! | TTACACATTTTGTGATTAGT+AGG | - | chr3.3:35028080-35028099 | None:intergenic | 25.0% |
| !! | TTTTAAACACAGTAGATTCT+AGG | - | chr3.3:35027642-35027661 | None:intergenic | 25.0% |
| GTGTACAAATCTTTGAACAT+AGG | + | chr3.3:35027516-35027535 | MS.gene50924:intron | 30.0% | |
| TATAATGTGTCATACCACAA+AGG | + | chr3.3:35027988-35028007 | MS.gene50924:intron | 30.0% | |
| TGATACAACGTTATATGTCT+TGG | + | chr3.3:35027739-35027758 | MS.gene50924:intron | 30.0% | |
| TTCTTAATCAGGTGTTAACA+AGG | + | chr3.3:35028119-35028138 | MS.gene50924:intron | 30.0% | |
| TTGGATTTAATCGATATGCA+CGG | + | chr3.3:35027758-35027777 | MS.gene50924:intron | 30.0% | |
| ! | GGAATCGAACTCAAAATTTT+AGG | - | chr3.3:35028059-35028078 | None:intergenic | 30.0% |
| ATTCAATGCTTACCATGAAC+AGG | - | chr3.3:35027419-35027438 | None:intergenic | 35.0% | |
| CCTTGAGATATCCAAAAAAG+TGG | + | chr3.3:35027710-35027729 | MS.gene50924:intron | 35.0% | |
| GATATCCAAAAAAGTGGATG+TGG | + | chr3.3:35027716-35027735 | MS.gene50924:intron | 35.0% | |
| TTGAGGTTAACATATTGTGC+TGG | - | chr3.3:35028228-35028247 | None:intergenic | 35.0% | |
| ! | CTGTCAGAAACTTTTGATCT+TGG | + | chr3.3:35027287-35027306 | MS.gene50924:CDS | 35.0% |
| !!! | CCACTTTTTTGGATATCTCA+AGG | - | chr3.3:35027713-35027732 | None:intergenic | 35.0% |
| !!! | TATCACCACATCCACTTTTT+TGG | - | chr3.3:35027724-35027743 | None:intergenic | 35.0% |
| AGATCTTGTCACCAAAGGAA+AGG | + | chr3.3:35028160-35028179 | MS.gene50924:CDS | 40.0% | |
| AGTTATGGATGATCCTGTTG+AGG | + | chr3.3:35027385-35027404 | MS.gene50924:CDS | 40.0% | |
| ATCTTGTCACCAAAGGAAAG+GGG | + | chr3.3:35028162-35028181 | MS.gene50924:CDS | 40.0% | |
| CATCAAGATCTTGTCACCAA+AGG | + | chr3.3:35028155-35028174 | MS.gene50924:CDS | 40.0% | |
| GAGGAAAAAAAGCCTGTTCA+TGG | + | chr3.3:35027404-35027423 | MS.gene50924:CDS | 40.0% | |
| GATCTGTGTTTGTGTTTGGT+CGG | + | chr3.3:35027811-35027830 | MS.gene50924:intron | 40.0% | |
| GATCTTGTCACCAAAGGAAA+GGG | + | chr3.3:35028161-35028180 | MS.gene50924:CDS | 40.0% | |
| TCAACAGGATCATCCATAAC+TGG | - | chr3.3:35027386-35027405 | None:intergenic | 40.0% | |
| ! | AAAGGTGTAGTGCAAGTAGT+TGG | + | chr3.3:35028006-35028025 | MS.gene50924:intron | 40.0% |
| !! | AAGTGCTTTGGAGATCCAAT+AGG | - | chr3.3:35028198-35028217 | None:intergenic | 40.0% |
| !! | AGTGCTTTGGAGATCCAATA+GGG | - | chr3.3:35028197-35028216 | None:intergenic | 40.0% |
| !!! | ACAGGCTTTTTTTCCTCAAC+AGG | - | chr3.3:35027401-35027420 | None:intergenic | 40.0% |
| CTACTTGCACTACACCTTTG+TGG | - | chr3.3:35028005-35028024 | None:intergenic | 45.0% | |
| GGGTGATCTGTGTTTGTGTT+TGG | + | chr3.3:35027807-35027826 | MS.gene50924:intron | 45.0% | |
| TGGACACTCTTGAGCTTCAT+CGG | - | chr3.3:35027366-35027385 | None:intergenic | 45.0% | |
| !! | AGCTCAAGAGTGTCCAGTTA+TGG | + | chr3.3:35027370-35027389 | MS.gene50924:CDS | 45.0% |
| !! | CGAGGTTGGTGAATTCTTTG+AGG | - | chr3.3:35028258-35028277 | None:intergenic | 45.0% |
| !! | GTGCTTTGGAGATCCAATAG+GGG | - | chr3.3:35028196-35028215 | None:intergenic | 45.0% |
| !! | TTCTTTGAGGTGCACACTTG+AGG | - | chr3.3:35028245-35028264 | None:intergenic | 45.0% |
| GACTTTGGACCCCTTTCCTT+TGG | - | chr3.3:35028174-35028193 | None:intergenic | 50.0% | |
| GGAGATCCAATAGGGGACTT+TGG | - | chr3.3:35028189-35028208 | None:intergenic | 50.0% | |
| ! | GCTGGCTTCTCAAAGTGCTT+TGG | - | chr3.3:35028210-35028229 | None:intergenic | 50.0% |
| AGGGGTCCAAAGTCCCCTAT+TGG | + | chr3.3:35028180-35028199 | MS.gene50924:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 35027279 | 35028281 | 35027279 | ID=MS.gene50924 |
| chr3.3 | mRNA | 35027279 | 35028281 | 35027279 | ID=MS.gene50924.t1;Parent=MS.gene50924 |
| chr3.3 | exon | 35027279 | 35027425 | 35027279 | ID=MS.gene50924.t1.exon1;Parent=MS.gene50924.t1 |
| chr3.3 | CDS | 35027279 | 35027425 | 35027279 | ID=cds.MS.gene50924.t1;Parent=MS.gene50924.t1 |
| chr3.3 | exon | 35028130 | 35028281 | 35028130 | ID=MS.gene50924.t1.exon2;Parent=MS.gene50924.t1 |
| chr3.3 | CDS | 35028130 | 35028281 | 35028130 | ID=cds.MS.gene50924.t1;Parent=MS.gene50924.t1 |
| Gene Sequence |
| Protein sequence |
