Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057992.t1 | XP_003598896.1 | 97.7 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 6.20E-43 | 183 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057992.t1 | Q39096 | 53.9 | 89 | 38 | 2 | 1 | 86 | 1 | 89 | 8.2e-20 | 97.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057992.t1 | B7FH52 | 97.7 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 4.5e-43 | 183.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057989 | MS.gene057992 | 0.938631 | 4.89E-99 | -1.69E-46 |
| MS.gene057992 | MS.gene058774 | -0.801838 | 7.45E-49 | -1.69E-46 |
| MS.gene057992 | MS.gene50922 | 0.944417 | 2.03E-103 | -1.69E-46 |
| MS.gene057992 | MS.gene50924 | 0.85783 | 1.25E-62 | -1.69E-46 |
| MS.gene057992 | MS.gene50926 | 0.944502 | 1.73E-103 | -1.69E-46 |
| MS.gene057992 | MS.gene61320 | 0.803631 | 3.18E-49 | -1.69E-46 |
| MS.gene057992 | MS.gene65812 | -0.832258 | 1.05E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057992.t1 | MTR_3g023110 | 97.727 | 88 | 2 | 0 | 1 | 88 | 1 | 88 | 5.69e-61 | 183 |
| MS.gene057992.t1 | MTR_7g090630 | 52.809 | 89 | 39 | 2 | 1 | 88 | 1 | 87 | 9.79e-27 | 94.4 |
| MS.gene057992.t1 | MTR_7g090630 | 51.685 | 89 | 40 | 2 | 1 | 88 | 1 | 87 | 1.76e-26 | 94.0 |
| MS.gene057992.t1 | MTR_5g083690 | 54.902 | 51 | 23 | 0 | 1 | 51 | 4 | 54 | 1.72e-15 | 66.6 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057992.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 3.03e-25 | 91.3 |
| MS.gene057992.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 7.28e-25 | 91.3 |
| MS.gene057992.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 7.28e-25 | 91.3 |
| MS.gene057992.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 7.28e-25 | 91.3 |
| MS.gene057992.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 7.28e-25 | 91.3 |
| MS.gene057992.t1 | AT4G14270 | 45.455 | 77 | 39 | 2 | 2 | 77 | 3 | 77 | 3.95e-19 | 76.3 |
| MS.gene057992.t1 | AT4G14270 | 45.455 | 77 | 39 | 2 | 2 | 77 | 3 | 77 | 3.95e-19 | 76.3 |
Find 13 sgRNAs with CRISPR-Local
Find 17 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CGACATGGTTTCATAGCTAT+TGG | 0.303499 | 3.3:+35013443 | MS.gene057992:CDS |
| GTTCTGATGGCATTGGTAAC+TGG | 0.375492 | 3.3:+35013319 | None:intergenic |
| GGTATGAAGAGAGGTGCATT+TGG | 0.424438 | 3.3:-35013361 | None:intergenic |
| CTATTGGTTGAGCCAGCATA+AGG | 0.514718 | 3.3:+35013459 | MS.gene057992:CDS |
| CAAAATCTTCCTCCTTATGC+TGG | 0.523223 | 3.3:-35013471 | None:intergenic |
| ACCTGCTGCATTTCGTCAAG+TGG | 0.534431 | 3.3:+35013381 | MS.gene057992:CDS |
| TTCACAAGCTCCCACCATTG+AGG | 0.540449 | 3.3:-35013415 | None:intergenic |
| TCCACTTGACGAAATGCAGC+AGG | 0.554768 | 3.3:-35013382 | None:intergenic |
| TGCTGCATTTCGTCAAGTGG+AGG | 0.583030 | 3.3:+35013384 | MS.gene057992:CDS |
| AGCTTGTGAAATCTTCGACA+TGG | 0.599712 | 3.3:+35013428 | MS.gene057992:CDS |
| CTGACAAGTTCTGATGGCAT+TGG | 0.609871 | 3.3:+35013312 | None:intergenic |
| TTGGTTGAGCCAGCATAAGG+AGG | 0.633628 | 3.3:+35013462 | MS.gene057992:CDS |
| AATGCAGCAGGTATGAAGAG+AGG | 0.675950 | 3.3:-35013370 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | CTGTCAGAAACTTTTGATCT+TGG | + | chr3.3:35013544-35013563 | MS.gene057992:CDS | 35.0% |
| AGCTTGTGAAATCTTCGACA+TGG | + | chr3.3:35013428-35013447 | MS.gene057992:CDS | 40.0% | |
| CAAAATCTTCCTCCTTATGC+TGG | - | chr3.3:35013474-35013493 | None:intergenic | 40.0% | |
| CGACATGGTTTCATAGCTAT+TGG | + | chr3.3:35013443-35013462 | MS.gene057992:CDS | 40.0% | |
| ! | AGGATTTTTCACCTCAATGG+TGG | + | chr3.3:35013404-35013423 | MS.gene057992:CDS | 40.0% |
| ! | GAAGATTTTGTCGAAAGTGG+CGG | + | chr3.3:35013484-35013503 | MS.gene057992:CDS | 40.0% |
| ! | GAGGAAGATTTTGTCGAAAG+TGG | + | chr3.3:35013481-35013500 | MS.gene057992:CDS | 40.0% |
| ! | GGATTTTTCACCTCAATGGT+GGG | + | chr3.3:35013405-35013424 | MS.gene057992:CDS | 40.0% |
| ! | TGGAGGATTTTTCACCTCAA+TGG | + | chr3.3:35013401-35013420 | MS.gene057992:CDS | 40.0% |
| AATGCAGCAGGTATGAAGAG+AGG | - | chr3.3:35013373-35013392 | None:intergenic | 45.0% | |
| ! | CTATTGGTTGAGCCAGCATA+AGG | + | chr3.3:35013459-35013478 | MS.gene057992:CDS | 45.0% |
| ! | GGTATGAAGAGAGGTGCATT+TGG | - | chr3.3:35013364-35013383 | None:intergenic | 45.0% |
| ACCTGCTGCATTTCGTCAAG+TGG | + | chr3.3:35013381-35013400 | MS.gene057992:CDS | 50.0% | |
| TCCACTTGACGAAATGCAGC+AGG | - | chr3.3:35013385-35013404 | None:intergenic | 50.0% | |
| TGCTGCATTTCGTCAAGTGG+AGG | + | chr3.3:35013384-35013403 | MS.gene057992:CDS | 50.0% | |
| TTCACAAGCTCCCACCATTG+AGG | - | chr3.3:35013418-35013437 | None:intergenic | 50.0% | |
| TTGGTTGAGCCAGCATAAGG+AGG | + | chr3.3:35013462-35013481 | MS.gene057992:CDS | 50.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 35013325 | 35013591 | 35013325 | ID=MS.gene057992 |
| chr3.3 | mRNA | 35013325 | 35013591 | 35013325 | ID=MS.gene057992.t1;Parent=MS.gene057992 |
| chr3.3 | exon | 35013325 | 35013591 | 35013325 | ID=MS.gene057992.t1.exon1;Parent=MS.gene057992.t1 |
| chr3.3 | CDS | 35013325 | 35013591 | 35013325 | ID=cds.MS.gene057992.t1;Parent=MS.gene057992.t1 |
| Gene Sequence |
| Protein sequence |
