Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057989.t1 | XP_003598896.1 | 97.4 | 116 | 3 | 0 | 1 | 116 | 1 | 116 | 8.80E-59 | 236.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057989.t1 | Q39096 | 53.9 | 89 | 38 | 2 | 1 | 86 | 1 | 89 | 1.5e-19 | 97.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057989.t1 | B7FH52 | 97.4 | 116 | 3 | 0 | 1 | 116 | 1 | 116 | 6.3e-59 | 236.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057989 | MS.gene057992 | 0.938631 | 4.89E-99 | -1.69E-46 |
| MS.gene057989 | MS.gene50922 | 0.918057 | 2.49E-86 | -1.69E-46 |
| MS.gene057989 | MS.gene50924 | 0.844664 | 6.57E-59 | -1.69E-46 |
| MS.gene057989 | MS.gene50926 | 0.921012 | 6.17E-88 | -1.69E-46 |
| MS.gene057989 | MS.gene65812 | -0.849411 | 3.29E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057989.t1 | MTR_3g023110 | 94.215 | 121 | 7 | 0 | 1 | 121 | 1 | 121 | 7.11e-82 | 237 |
| MS.gene057989.t1 | MTR_7g090630 | 49.524 | 105 | 49 | 3 | 1 | 104 | 1 | 102 | 4.55e-29 | 102 |
| MS.gene057989.t1 | MTR_7g090630 | 50.000 | 104 | 48 | 3 | 1 | 103 | 1 | 101 | 1.40e-28 | 100 |
| MS.gene057989.t1 | MTR_5g083690 | 56.000 | 50 | 22 | 0 | 1 | 50 | 4 | 53 | 2.64e-15 | 67.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene057989.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 5.39e-25 | 92.0 |
| MS.gene057989.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 1.08e-24 | 92.4 |
| MS.gene057989.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 1.08e-24 | 92.4 |
| MS.gene057989.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 1.08e-24 | 92.4 |
| MS.gene057989.t1 | AT2G41430 | 53.750 | 80 | 34 | 2 | 1 | 77 | 1 | 80 | 1.08e-24 | 92.4 |
| MS.gene057989.t1 | AT4G14270 | 36.667 | 120 | 49 | 3 | 2 | 121 | 3 | 95 | 4.42e-19 | 77.4 |
| MS.gene057989.t1 | AT4G14270 | 36.667 | 120 | 49 | 3 | 2 | 121 | 3 | 95 | 4.42e-19 | 77.4 |
Find 20 sgRNAs with CRISPR-Local
Find 71 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGATAATGAGTTTGAGAAAT+TGG | 0.215472 | 3.4:+14580561 | MS.gene057989:CDS |
| CGACATGGTTTCATAGCTAT+TGG | 0.303499 | 3.4:+14580413 | MS.gene057989:CDS |
| GTTCTGATGGCATTGGTAAC+TGG | 0.375492 | 3.4:+14580289 | None:intergenic |
| ATTCAATGCTTACCATGAAC+AGG | 0.405358 | 3.4:-14580644 | None:intergenic |
| GGTATGAAGAGAGGTGCATT+TGG | 0.424438 | 3.4:-14580331 | None:intergenic |
| TCAACAGGATCATCCATAAC+TGG | 0.427432 | 3.4:-14580610 | None:intergenic |
| CATGTAGTGTATTTAATGGT+AGG | 0.436501 | 3.4:+14581645 | MS.gene057989:intron |
| AGCTCAAGAGTGTCCAGTTA+TGG | 0.459111 | 3.4:+14580597 | MS.gene057989:CDS |
| TGGACACTCTTGAGCTTCAT+CGG | 0.502009 | 3.4:-14580590 | None:intergenic |
| CTATTGGTTGAGCCAGCATA+AGG | 0.514718 | 3.4:+14580429 | MS.gene057989:CDS |
| CAAAATCTTCCTCCTTATGC+TGG | 0.523223 | 3.4:-14580441 | None:intergenic |
| ACCTGCTGCATTTCGTCAAG+TGG | 0.534431 | 3.4:+14580351 | MS.gene057989:CDS |
| TTCACAAGCTCCCACCATTG+AGG | 0.540449 | 3.4:-14580385 | None:intergenic |
| TCCACTTGACGAAATGCAGC+AGG | 0.554768 | 3.4:-14580352 | None:intergenic |
| TGCTGCATTTCGTCAAGTGG+AGG | 0.583030 | 3.4:+14580354 | MS.gene057989:CDS |
| AGCTTGTGAAATCTTCGACA+TGG | 0.599712 | 3.4:+14580398 | MS.gene057989:CDS |
| CTGACAAGTTCTGATGGCAT+TGG | 0.609871 | 3.4:+14580282 | None:intergenic |
| AGTTATGGATGATCCTGTTG+AGG | 0.618736 | 3.4:+14580612 | MS.gene057989:CDS |
| TTGGTTGAGCCAGCATAAGG+AGG | 0.633628 | 3.4:+14580432 | MS.gene057989:CDS |
| AATGCAGCAGGTATGAAGAG+AGG | 0.675950 | 3.4:-14580340 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTATAATGATCAAATATAAA+CGG | - | chr3.4:14580698-14580717 | None:intergenic | 10.0% |
| !! | TAGTTTAAAAATCATACAAA+GGG | + | chr3.4:14581016-14581035 | MS.gene057989:intron | 15.0% |
| !!! | AATACTTTTTCAAACTAAAT+AGG | + | chr3.4:14580915-14580934 | MS.gene057989:intron | 15.0% |
| !!! | ATTATATTTACTCTAACTTT+TGG | - | chr3.4:14581203-14581222 | None:intergenic | 15.0% |
| !! | CTAGTTTAAAAATCATACAA+AGG | + | chr3.4:14581015-14581034 | MS.gene057989:intron | 20.0% |
| !!! | AATTGATTTCTTTCTTAATC+AGG | + | chr3.4:14581337-14581356 | MS.gene057989:intron | 20.0% |
| !!! | AGATCAATTTTATACATTGT+CGG | - | chr3.4:14581085-14581104 | None:intergenic | 20.0% |
| !!! | TTTTCATGTAGTGTATTTAA+TGG | + | chr3.4:14581641-14581660 | MS.gene057989:intron | 20.0% |
| ! | AGATAATGAGTTTGAGAAAT+TGG | + | chr3.4:14580561-14580580 | MS.gene057989:CDS | 25.0% |
| !! | GAATCGAACTCAAAATTTTA+GGG | - | chr3.4:14581287-14581306 | None:intergenic | 25.0% |
| !! | TAACTTGTGTATATAGAAGA+AGG | + | chr3.4:14581587-14581606 | MS.gene057989:intron | 25.0% |
| !! | TGTCTTTGTATTTTTCTTGT+TGG | + | chr3.4:14581618-14581637 | MS.gene057989:intron | 25.0% |
| !! | TTACACATTTTGTGATTAGT+AGG | - | chr3.4:14581309-14581328 | None:intergenic | 25.0% |
| !! | TTTTAAACACAGTAGATTCT+AGG | - | chr3.4:14580870-14580889 | None:intergenic | 25.0% |
| CATGTAGTGTATTTAATGGT+AGG | + | chr3.4:14581645-14581664 | MS.gene057989:intron | 30.0% | |
| TATAATGTGTCATACCACAA+AGG | + | chr3.4:14581217-14581236 | MS.gene057989:intron | 30.0% | |
| TGATACAACGTTATATGTCT+TGG | + | chr3.4:14580968-14580987 | MS.gene057989:intron | 30.0% | |
| TGTGTACAAATCTTGAACAT+AGG | + | chr3.4:14580743-14580762 | MS.gene057989:intron | 30.0% | |
| TTCTTAATCAGGTGTTAACA+AGG | + | chr3.4:14581348-14581367 | MS.gene057989:intron | 30.0% | |
| TTGGATTTAATCGATATGCA+CGG | + | chr3.4:14580987-14581006 | MS.gene057989:intron | 30.0% | |
| ! | GGAATCGAACTCAAAATTTT+AGG | - | chr3.4:14581288-14581307 | None:intergenic | 30.0% |
| AAAGTTTATCCTAACGAGGT+TGG | - | chr3.4:14581501-14581520 | None:intergenic | 35.0% | |
| AGGAAAAAAAAGCCTGTTCA+TGG | + | chr3.4:14580632-14580651 | MS.gene057989:CDS | 35.0% | |
| ATTCAATGCTTACCATGAAC+AGG | - | chr3.4:14580647-14580666 | None:intergenic | 35.0% | |
| CCTTGAGATATCCAAAAAAG+TGG | + | chr3.4:14580939-14580958 | MS.gene057989:intron | 35.0% | |
| CTTCAAAGTTTATCCTAACG+AGG | - | chr3.4:14581505-14581524 | None:intergenic | 35.0% | |
| GATATCCAAAAAAGTGGATG+TGG | + | chr3.4:14580945-14580964 | MS.gene057989:intron | 35.0% | |
| TTGAGGTTAACATATTGTGC+TGG | - | chr3.4:14581457-14581476 | None:intergenic | 35.0% | |
| TTGCAGTTCAAGCAAGTTAT+CGG | - | chr3.4:14581534-14581553 | None:intergenic | 35.0% | |
| ! | CTGTCAGAAACTTTTGATCT+TGG | + | chr3.4:14580514-14580533 | MS.gene057989:CDS | 35.0% |
| !!! | CCACTTTTTTGGATATCTCA+AGG | - | chr3.4:14580942-14580961 | None:intergenic | 35.0% |
| !!! | TATCACCACATCCACTTTTT+TGG | - | chr3.4:14580953-14580972 | None:intergenic | 35.0% |
| AAGAATTCACCAACCTCGTT+AGG | + | chr3.4:14581489-14581508 | MS.gene057989:intron | 40.0% | |
| AGATCTTGTCACCAAAGGAA+AGG | + | chr3.4:14581389-14581408 | MS.gene057989:intron | 40.0% | |
| AGCTTGTGAAATCTTCGACA+TGG | + | chr3.4:14580398-14580417 | MS.gene057989:CDS | 40.0% | |
| AGTTATGGATGATCCTGTTG+AGG | + | chr3.4:14580612-14580631 | MS.gene057989:CDS | 40.0% | |
| ATCTTGTCACCAAAGGAAAG+GGG | + | chr3.4:14581391-14581410 | MS.gene057989:intron | 40.0% | |
| CAAAATCTTCCTCCTTATGC+TGG | - | chr3.4:14580444-14580463 | None:intergenic | 40.0% | |
| CATCAAGATCTTGTCACCAA+AGG | + | chr3.4:14581384-14581403 | MS.gene057989:intron | 40.0% | |
| CGACATGGTTTCATAGCTAT+TGG | + | chr3.4:14580413-14580432 | MS.gene057989:CDS | 40.0% | |
| GATCTGTGTTTGTGTTTGGT+CGG | + | chr3.4:14581040-14581059 | MS.gene057989:intron | 40.0% | |
| GATCTTGTCACCAAAGGAAA+GGG | + | chr3.4:14581390-14581409 | MS.gene057989:intron | 40.0% | |
| TCAACAGGATCATCCATAAC+TGG | - | chr3.4:14580613-14580632 | None:intergenic | 40.0% | |
| ! | AAAGGTGTAGTGCAAGTAGT+TGG | + | chr3.4:14581235-14581254 | MS.gene057989:intron | 40.0% |
| ! | AGGATTTTTCACCTCAATGG+TGG | + | chr3.4:14580374-14580393 | MS.gene057989:CDS | 40.0% |
| ! | GAAGATTTTGTCGAAAGTGG+CGG | + | chr3.4:14580454-14580473 | MS.gene057989:CDS | 40.0% |
| ! | GAGGAAGATTTTGTCGAAAG+TGG | + | chr3.4:14580451-14580470 | MS.gene057989:CDS | 40.0% |
| ! | GGATTTTTCACCTCAATGGT+GGG | + | chr3.4:14580375-14580394 | MS.gene057989:CDS | 40.0% |
| ! | TGGAGGATTTTTCACCTCAA+TGG | + | chr3.4:14580371-14580390 | MS.gene057989:CDS | 40.0% |
| !! | AAGTGCTTTGGAGATCCAAT+AGG | - | chr3.4:14581427-14581446 | None:intergenic | 40.0% |
| !! | AGTGCTTTGGAGATCCAATA+GGG | - | chr3.4:14581426-14581445 | None:intergenic | 40.0% |
| !!! | CAGGCTTTTTTTTCCTCAAC+AGG | - | chr3.4:14580628-14580647 | None:intergenic | 40.0% |
| AATGCAGCAGGTATGAAGAG+AGG | - | chr3.4:14580343-14580362 | None:intergenic | 45.0% | |
| CTACTTGCACTACACCTTTG+TGG | - | chr3.4:14581234-14581253 | None:intergenic | 45.0% | |
| GGGTGATCTGTGTTTGTGTT+TGG | + | chr3.4:14581036-14581055 | MS.gene057989:intron | 45.0% | |
| TGGACACTCTTGAGCTTCAT+CGG | - | chr3.4:14580593-14580612 | None:intergenic | 45.0% | |
| ! | CTATTGGTTGAGCCAGCATA+AGG | + | chr3.4:14580429-14580448 | MS.gene057989:CDS | 45.0% |
| ! | GGTATGAAGAGAGGTGCATT+TGG | - | chr3.4:14580334-14580353 | None:intergenic | 45.0% |
| !! | AGCTCAAGAGTGTCCAGTTA+TGG | + | chr3.4:14580597-14580616 | MS.gene057989:CDS | 45.0% |
| !! | CGAGGTTGGTGAATTCTTTG+AGG | - | chr3.4:14581487-14581506 | None:intergenic | 45.0% |
| !! | GTGCTTTGGAGATCCAATAG+GGG | - | chr3.4:14581425-14581444 | None:intergenic | 45.0% |
| !! | TTCTTTGAGGTGCACACTTG+AGG | - | chr3.4:14581474-14581493 | None:intergenic | 45.0% |
| ACCTGCTGCATTTCGTCAAG+TGG | + | chr3.4:14580351-14580370 | MS.gene057989:CDS | 50.0% | |
| GACTTTGGACCCCTTTCCTT+TGG | - | chr3.4:14581403-14581422 | None:intergenic | 50.0% | |
| GGAGATCCAATAGGGGACTT+TGG | - | chr3.4:14581418-14581437 | None:intergenic | 50.0% | |
| TCCACTTGACGAAATGCAGC+AGG | - | chr3.4:14580355-14580374 | None:intergenic | 50.0% | |
| TGCTGCATTTCGTCAAGTGG+AGG | + | chr3.4:14580354-14580373 | MS.gene057989:CDS | 50.0% | |
| TTCACAAGCTCCCACCATTG+AGG | - | chr3.4:14580388-14580407 | None:intergenic | 50.0% | |
| TTGGTTGAGCCAGCATAAGG+AGG | + | chr3.4:14580432-14580451 | MS.gene057989:CDS | 50.0% | |
| ! | GCTGGCTTCTCAAAGTGCTT+TGG | - | chr3.4:14581439-14581458 | None:intergenic | 50.0% |
| AGGGGTCCAAAGTCCCCTAT+TGG | + | chr3.4:14581409-14581428 | MS.gene057989:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 14580295 | 14581670 | 14580295 | ID=MS.gene057989 |
| chr3.4 | mRNA | 14580295 | 14581670 | 14580295 | ID=MS.gene057989.t1;Parent=MS.gene057989 |
| chr3.4 | exon | 14580295 | 14580653 | 14580295 | ID=MS.gene057989.t1.exon1;Parent=MS.gene057989.t1 |
| chr3.4 | CDS | 14580295 | 14580653 | 14580295 | ID=cds.MS.gene057989.t1;Parent=MS.gene057989.t1 |
| chr3.4 | exon | 14581652 | 14581670 | 14581652 | ID=MS.gene057989.t1.exon2;Parent=MS.gene057989.t1 |
| chr3.4 | CDS | 14581652 | 14581670 | 14581652 | ID=cds.MS.gene057989.t1;Parent=MS.gene057989.t1 |
| Gene Sequence |
| Protein sequence |
