Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51003.t1 | XP_013459315.1 | 66.2 | 154 | 52 | 0 | 1 | 154 | 1 | 154 | 1.20E-52 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51003.t1 | P62929 | 54.1 | 122 | 49 | 4 | 1 | 122 | 1 | 115 | 8.4e-28 | 124.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51003.t1 | I3T793 | 66.2 | 154 | 52 | 0 | 1 | 154 | 1 | 154 | 8.3e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049633 | MS.gene51003 | -0.802882 | 4.54E-49 | -1.69E-46 |
| MS.gene051651 | MS.gene51003 | 0.804305 | 2.30E-49 | -1.69E-46 |
| MS.gene05589 | MS.gene51003 | -0.819801 | 9.60E-53 | -1.69E-46 |
| MS.gene056504 | MS.gene51003 | -0.802635 | 5.11E-49 | -1.69E-46 |
| MS.gene056697 | MS.gene51003 | 0.801734 | 7.83E-49 | -1.69E-46 |
| MS.gene056856 | MS.gene51003 | 0.810746 | 9.89E-51 | -1.69E-46 |
| MS.gene060405 | MS.gene51003 | 0.815865 | 7.42E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51003.t1 | MTR_3g436100 | 73.377 | 154 | 41 | 0 | 1 | 154 | 1 | 154 | 6.62e-82 | 238 |
| MS.gene51003.t1 | MTR_7g044920 | 54.777 | 157 | 63 | 4 | 1 | 154 | 1 | 152 | 5.75e-53 | 165 |
| MS.gene51003.t1 | MTR_7g044980 | 52.258 | 155 | 63 | 6 | 1 | 154 | 1 | 145 | 1.27e-46 | 149 |
| MS.gene51003.t1 | MTR_8g022430 | 49.664 | 149 | 58 | 5 | 1 | 148 | 1 | 133 | 9.82e-40 | 131 |
| MS.gene51003.t1 | MTR_6g017170 | 53.906 | 128 | 53 | 4 | 1 | 127 | 1 | 123 | 5.00e-38 | 126 |
| MS.gene51003.t1 | MTR_6g017150 | 49.333 | 150 | 57 | 6 | 1 | 148 | 1 | 133 | 4.08e-37 | 124 |
| MS.gene51003.t1 | MTR_8g022400 | 50.388 | 129 | 58 | 4 | 1 | 128 | 1 | 124 | 6.50e-36 | 121 |
| MS.gene51003.t1 | MTR_8g022420 | 50.000 | 138 | 60 | 5 | 1 | 136 | 1 | 131 | 4.15e-35 | 119 |
| MS.gene51003.t1 | MTR_3g067540 | 46.154 | 156 | 60 | 5 | 1 | 150 | 1 | 138 | 1.81e-34 | 117 |
| MS.gene51003.t1 | MTR_3g067555 | 46.154 | 156 | 60 | 5 | 1 | 150 | 1 | 138 | 1.81e-34 | 117 |
| MS.gene51003.t1 | MTR_7g056803 | 47.656 | 128 | 62 | 3 | 1 | 127 | 1 | 124 | 6.58e-34 | 116 |
| MS.gene51003.t1 | MTR_3g067437 | 48.507 | 134 | 60 | 3 | 1 | 132 | 1 | 127 | 6.95e-34 | 115 |
| MS.gene51003.t1 | MTR_3g067430 | 42.581 | 155 | 67 | 6 | 1 | 150 | 1 | 138 | 4.02e-33 | 114 |
| MS.gene51003.t1 | MTR_7g056817 | 49.231 | 130 | 58 | 6 | 1 | 127 | 1 | 125 | 6.37e-33 | 114 |
| MS.gene51003.t1 | MTR_6g036620 | 45.033 | 151 | 63 | 6 | 1 | 150 | 1 | 132 | 2.57e-30 | 107 |
| MS.gene51003.t1 | MTR_3g067270 | 45.255 | 137 | 67 | 5 | 1 | 136 | 1 | 130 | 1.10e-29 | 105 |
| MS.gene51003.t1 | MTR_3g467750 | 45.255 | 137 | 67 | 5 | 1 | 136 | 1 | 130 | 1.10e-29 | 105 |
| MS.gene51003.t1 | MTR_6g047900 | 44.805 | 154 | 64 | 8 | 1 | 153 | 1 | 134 | 1.46e-29 | 105 |
| MS.gene51003.t1 | MTR_3g067535 | 45.223 | 157 | 56 | 6 | 1 | 150 | 1 | 134 | 1.70e-29 | 105 |
| MS.gene51003.t1 | MTR_8g056800 | 55.556 | 81 | 36 | 0 | 74 | 154 | 21 | 101 | 4.24e-28 | 100 |
| MS.gene51003.t1 | MTR_3g067580 | 40.714 | 140 | 73 | 5 | 1 | 137 | 9 | 141 | 9.05e-28 | 100 |
| MS.gene51003.t1 | MTR_6g047880 | 41.558 | 154 | 72 | 6 | 1 | 153 | 1 | 137 | 4.42e-27 | 99.0 |
| MS.gene51003.t1 | MTR_3g463570 | 39.241 | 158 | 68 | 6 | 1 | 152 | 1 | 136 | 7.89e-27 | 98.2 |
| MS.gene51003.t1 | MTR_3g067510 | 44.715 | 123 | 56 | 3 | 1 | 119 | 1 | 115 | 9.67e-27 | 97.8 |
| MS.gene51003.t1 | MTR_3g067280 | 43.066 | 137 | 70 | 5 | 1 | 136 | 1 | 130 | 7.11e-26 | 95.9 |
| MS.gene51003.t1 | MTR_3g067445 | 41.463 | 123 | 61 | 4 | 1 | 118 | 1 | 117 | 1.08e-24 | 92.0 |
| MS.gene51003.t1 | MTR_3g067555 | 41.259 | 143 | 60 | 5 | 14 | 150 | 1 | 125 | 1.30e-24 | 92.4 |
| MS.gene51003.t1 | MTR_3g067510 | 42.188 | 128 | 57 | 4 | 1 | 119 | 1 | 120 | 6.72e-24 | 90.9 |
| MS.gene51003.t1 | MTR_6g082060 | 41.007 | 139 | 68 | 4 | 1 | 132 | 1 | 132 | 1.63e-23 | 90.1 |
| MS.gene51003.t1 | MTR_3g067550 | 42.537 | 134 | 53 | 5 | 23 | 150 | 2 | 117 | 2.01e-23 | 89.0 |
| MS.gene51003.t1 | MTR_3g067555 | 42.537 | 134 | 53 | 5 | 23 | 150 | 2 | 117 | 2.01e-23 | 89.0 |
| MS.gene51003.t1 | MTR_3g436120 | 41.791 | 134 | 24 | 2 | 21 | 154 | 22 | 101 | 3.37e-23 | 87.8 |
| MS.gene51003.t1 | MTR_3g467760 | 38.686 | 137 | 52 | 4 | 1 | 136 | 1 | 106 | 5.65e-23 | 87.8 |
| MS.gene51003.t1 | MTR_1g019650 | 44.355 | 124 | 52 | 6 | 1 | 124 | 1 | 107 | 2.85e-22 | 85.9 |
| MS.gene51003.t1 | MTR_4g026590 | 41.139 | 158 | 76 | 5 | 1 | 154 | 1 | 145 | 3.20e-22 | 86.7 |
| MS.gene51003.t1 | MTR_5g464350 | 66.129 | 62 | 21 | 0 | 1 | 62 | 1 | 62 | 3.80e-22 | 84.3 |
| MS.gene51003.t1 | MTR_5g464490 | 74.545 | 55 | 14 | 0 | 1 | 55 | 1 | 55 | 1.05e-21 | 83.2 |
| MS.gene51003.t1 | MTR_3g067500 | 46.512 | 86 | 45 | 1 | 49 | 134 | 23 | 107 | 2.49e-21 | 83.6 |
| MS.gene51003.t1 | MTR_3g067570 | 58.209 | 67 | 23 | 3 | 53 | 119 | 29 | 90 | 3.08e-20 | 80.1 |
| MS.gene51003.t1 | MTR_3g463570 | 42.718 | 103 | 46 | 3 | 51 | 152 | 66 | 156 | 3.55e-20 | 81.6 |
| MS.gene51003.t1 | MTR_7g029540 | 41.270 | 126 | 66 | 6 | 1 | 124 | 1 | 120 | 8.42e-20 | 79.7 |
| MS.gene51003.t1 | MTR_8g461020 | 34.899 | 149 | 67 | 6 | 3 | 144 | 4 | 129 | 3.98e-19 | 78.6 |
| MS.gene51003.t1 | MTR_0112s0050 | 63.492 | 63 | 22 | 1 | 1 | 63 | 1 | 62 | 1.34e-18 | 75.1 |
| MS.gene51003.t1 | MTR_3g067830 | 40.496 | 121 | 56 | 4 | 1 | 117 | 1 | 109 | 1.50e-18 | 76.3 |
| MS.gene51003.t1 | MTR_0416s0030 | 58.462 | 65 | 27 | 0 | 1 | 65 | 1 | 65 | 7.87e-18 | 73.2 |
| MS.gene51003.t1 | MTR_5g464390 | 60.656 | 61 | 24 | 0 | 1 | 61 | 1 | 61 | 8.48e-18 | 73.2 |
| MS.gene51003.t1 | MTR_5g464590 | 58.730 | 63 | 24 | 2 | 1 | 61 | 1 | 63 | 6.73e-17 | 70.9 |
| MS.gene51003.t1 | MTR_5g464540 | 55.385 | 65 | 28 | 1 | 1 | 65 | 1 | 64 | 1.52e-15 | 67.4 |
| MS.gene51003.t1 | MTR_0093s0090 | 57.895 | 57 | 23 | 1 | 1 | 57 | 1 | 56 | 1.81e-14 | 64.7 |
| MS.gene51003.t1 | MTR_0112s0040 | 81.081 | 37 | 6 | 1 | 1 | 36 | 1 | 37 | 2.00e-12 | 58.9 |
| MS.gene51003.t1 | MTR_3g438170 | 80.000 | 35 | 7 | 0 | 1 | 35 | 1 | 35 | 2.91e-12 | 58.2 |
| MS.gene51003.t1 | MTR_3g438140 | 80.000 | 35 | 7 | 0 | 1 | 35 | 1 | 35 | 2.91e-12 | 58.2 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 33 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATATTATGTTCAGAACTAAT+TGG | 0.232965 | 3.2:-30002082 | None:intergenic |
| CCTAATGCTGATATTGAATA+TGG | 0.285356 | 3.2:+30002190 | MS.gene51003:CDS |
| AGCAGGGAGCTGTCCGTCTC+AGG | 0.357261 | 3.2:+30001964 | MS.gene51003:CDS |
| TTATGTTAAGCTTGCTACTT+TGG | 0.363249 | 3.2:+30001682 | MS.gene51003:CDS |
| AGATTGTAGTTGTATCCCTT+GGG | 0.363389 | 3.2:+30002033 | MS.gene51003:CDS |
| CTGCTTCAACGTTCTTCGTC+TGG | 0.376847 | 3.2:-30001946 | None:intergenic |
| TTGAAGAAATTGTTTACTAC+AGG | 0.382004 | 3.2:-30002268 | None:intergenic |
| GAGATTGTAGTTGTATCCCT+TGG | 0.382260 | 3.2:+30002032 | MS.gene51003:CDS |
| AGACTACTTGAAGATGGCTT+TGG | 0.386657 | 3.2:+30002315 | MS.gene51003:CDS |
| GCAGAACGTTACTTCAAGAT+TGG | 0.399752 | 3.2:+30002238 | MS.gene51003:CDS |
| CCATATTCAATATCAGCATT+AGG | 0.437256 | 3.2:-30002190 | None:intergenic |
| CAAGTCAAATATTGAACAAA+AGG | 0.441013 | 3.2:-30001990 | None:intergenic |
| TGAGAAATATCATTCTTAAG+TGG | 0.452118 | 3.2:-30002344 | None:intergenic |
| GAAATTGTTTACTACAGGGT+TGG | 0.463585 | 3.2:-30002263 | None:intergenic |
| CAGACGAAGAACGTTGAAGC+AGG | 0.486278 | 3.2:+30001947 | MS.gene51003:CDS |
| GAGTGCATCAAGAAGGGAAG+TGG | 0.522512 | 3.2:+30002148 | MS.gene51003:CDS |
| TCATGCCGAGTGCATCAAGA+AGG | 0.559241 | 3.2:+30002141 | MS.gene51003:CDS |
| GATTGTAGTTGTATCCCTTG+GGG | 0.572574 | 3.2:+30002034 | MS.gene51003:CDS |
| GCAGGGAGCTGTCCGTCTCA+GGG | 0.576016 | 3.2:+30001965 | MS.gene51003:CDS |
| ACTTCCCTTCTTGATGCACT+CGG | 0.605609 | 3.2:-30002146 | None:intergenic |
| ACAAATATACATACAGAATG+TGG | 0.609862 | 3.2:-30001720 | None:intergenic |
| TGAAGAAATTGTTTACTACA+GGG | 0.620608 | 3.2:-30002267 | None:intergenic |
| ATATTTGACTTGACACCATG+CGG | 0.621402 | 3.2:+30002001 | MS.gene51003:CDS |
| CGTTCTGCTTCATCTACAGA+GGG | 0.621559 | 3.2:-30002223 | None:intergenic |
| CATGCCGAGTGCATCAAGAA+GGG | 0.625240 | 3.2:+30002142 | MS.gene51003:CDS |
| GAACAAAAGGAACCCTGAGA+CGG | 0.632996 | 3.2:-30001977 | None:intergenic |
| AGACGAAGAACGTTGAAGCA+GGG | 0.637674 | 3.2:+30001948 | MS.gene51003:CDS |
| ATGCTGATATTGAATATGGA+TGG | 0.639133 | 3.2:+30002194 | MS.gene51003:CDS |
| AGTGCATCAAGAAGGGAAGT+GGG | 0.648471 | 3.2:+30002149 | MS.gene51003:CDS |
| CAATCTCTGAGATTGCCGCA+TGG | 0.655920 | 3.2:-30002016 | None:intergenic |
| ACGTTCTGCTTCATCTACAG+AGG | 0.657683 | 3.2:-30002224 | None:intergenic |
| ATTGTAGTTGTATCCCTTGG+GGG | 0.670258 | 3.2:+30002035 | MS.gene51003:CDS |
| ACATAATATGAAGATGATCG+AGG | 0.692790 | 3.2:+30002096 | MS.gene51003:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATGTATATTTGTTTAACATT+GGG | + | chr3.2:30001731-30001750 | MS.gene51003:intron | 15.0% |
| !! | TATGTATATTTGTTTAACAT+TGG | + | chr3.2:30001730-30001749 | MS.gene51003:intron | 15.0% |
| !! | ATATTATGTTCAGAACTAAT+TGG | - | chr3.2:30002085-30002104 | None:intergenic | 20.0% |
| !! | TCGATATATATTTATGTGTT+AGG | + | chr3.2:30001767-30001786 | MS.gene51003:intron | 20.0% |
| !! | TGTATATATTTACGAAAGTT+TGG | - | chr3.2:30001869-30001888 | None:intergenic | 20.0% |
| ! | ACAAATATACATACAGAATG+TGG | - | chr3.2:30001723-30001742 | None:intergenic | 25.0% |
| ! | CAAGTCAAATATTGAACAAA+AGG | - | chr3.2:30001993-30002012 | None:intergenic | 25.0% |
| ! | CACATAAATATATATCGATG+AGG | - | chr3.2:30001765-30001784 | None:intergenic | 25.0% |
| ! | TGAAGAAATTGTTTACTACA+GGG | - | chr3.2:30002270-30002289 | None:intergenic | 25.0% |
| ! | TTGAAGAAATTGTTTACTAC+AGG | - | chr3.2:30002271-30002290 | None:intergenic | 25.0% |
| ACATAATATGAAGATGATCG+AGG | + | chr3.2:30002096-30002115 | MS.gene51003:CDS | 30.0% | |
| ATGCTGATATTGAATATGGA+TGG | + | chr3.2:30002194-30002213 | MS.gene51003:CDS | 30.0% | |
| CCATATTCAATATCAGCATT+AGG | - | chr3.2:30002193-30002212 | None:intergenic | 30.0% | |
| CCTAATGCTGATATTGAATA+TGG | + | chr3.2:30002190-30002209 | MS.gene51003:CDS | 30.0% | |
| TACATTATTCACACGTGTAT+CGG | + | chr3.2:30001885-30001904 | MS.gene51003:intron | 30.0% | |
| TGAAAAAGACTACTTGAAGA+TGG | + | chr3.2:30002309-30002328 | MS.gene51003:CDS | 30.0% | |
| !! | GTCTTTTTCATTAGAGTTTG+AGG | - | chr3.2:30002299-30002318 | None:intergenic | 30.0% |
| !! | TTATGTTAAGCTTGCTACTT+TGG | + | chr3.2:30001682-30001701 | MS.gene51003:CDS | 30.0% |
| AGATTGTAGTTGTATCCCTT+GGG | + | chr3.2:30002033-30002052 | MS.gene51003:CDS | 35.0% | |
| ATGGCAAAGGCAGTTAAAAT+TGG | - | chr3.2:30001913-30001932 | None:intergenic | 35.0% | |
| GAAATTGTTTACTACAGGGT+TGG | - | chr3.2:30002266-30002285 | None:intergenic | 35.0% | |
| ! | GCATGAGTTTTGCATAAGTT+AGG | - | chr3.2:30002127-30002146 | None:intergenic | 35.0% |
| !! | ATATTTGACTTGACACCATG+CGG | + | chr3.2:30002001-30002020 | MS.gene51003:CDS | 35.0% |
| ACGATTACTGCAAATGGCAA+AGG | - | chr3.2:30001926-30001945 | None:intergenic | 40.0% | |
| AGACTACTTGAAGATGGCTT+TGG | + | chr3.2:30002315-30002334 | MS.gene51003:CDS | 40.0% | |
| ATTGTAGTTGTATCCCTTGG+GGG | + | chr3.2:30002035-30002054 | MS.gene51003:CDS | 40.0% | |
| GAGATTGTAGTTGTATCCCT+TGG | + | chr3.2:30002032-30002051 | MS.gene51003:CDS | 40.0% | |
| GATTGTAGTTGTATCCCTTG+GGG | + | chr3.2:30002034-30002053 | MS.gene51003:CDS | 40.0% | |
| GCAGAACGTTACTTCAAGAT+TGG | + | chr3.2:30002238-30002257 | MS.gene51003:CDS | 40.0% | |
| TACCAAAAAATAGCCCCCAA+GGG | - | chr3.2:30002051-30002070 | None:intergenic | 40.0% | |
| ACGTTCTGCTTCATCTACAG+AGG | - | chr3.2:30002227-30002246 | None:intergenic | 45.0% | |
| ACTTCCCTTCTTGATGCACT+CGG | - | chr3.2:30002149-30002168 | None:intergenic | 45.0% | |
| AGACGAAGAACGTTGAAGCA+GGG | + | chr3.2:30001948-30001967 | MS.gene51003:CDS | 45.0% | |
| AGTGCATCAAGAAGGGAAGT+GGG | + | chr3.2:30002149-30002168 | MS.gene51003:CDS | 45.0% | |
| CGTTCTGCTTCATCTACAGA+GGG | - | chr3.2:30002226-30002245 | None:intergenic | 45.0% | |
| GAACAAAAGGAACCCTGAGA+CGG | - | chr3.2:30001980-30001999 | None:intergenic | 45.0% | |
| GTACCAAAAAATAGCCCCCA+AGG | - | chr3.2:30002052-30002071 | None:intergenic | 45.0% | |
| GTCTGGACGATTACTGCAAA+TGG | - | chr3.2:30001932-30001951 | None:intergenic | 45.0% | |
| !!! | ATCCCTTGGGGGCTATTTTT+TGG | + | chr3.2:30002046-30002065 | MS.gene51003:CDS | 45.0% |
| CAGACGAAGAACGTTGAAGC+AGG | + | chr3.2:30001947-30001966 | MS.gene51003:CDS | 50.0% | |
| CATGCCGAGTGCATCAAGAA+GGG | + | chr3.2:30002142-30002161 | MS.gene51003:CDS | 50.0% | |
| CTGCTTCAACGTTCTTCGTC+TGG | - | chr3.2:30001949-30001968 | None:intergenic | 50.0% | |
| GAGTGCATCAAGAAGGGAAG+TGG | + | chr3.2:30002148-30002167 | MS.gene51003:CDS | 50.0% | |
| TCATGCCGAGTGCATCAAGA+AGG | + | chr3.2:30002141-30002160 | MS.gene51003:CDS | 50.0% | |
| !! | CAATCTCTGAGATTGCCGCA+TGG | - | chr3.2:30002019-30002038 | None:intergenic | 50.0% |
| AGCAGGGAGCTGTCCGTCTC+AGG | + | chr3.2:30001964-30001983 | MS.gene51003:CDS | 65.0% | |
| GCAGGGAGCTGTCCGTCTCA+GGG | + | chr3.2:30001965-30001984 | MS.gene51003:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 30001677 | 30002351 | 30001677 | ID=MS.gene51003 |
| chr3.2 | mRNA | 30001677 | 30002351 | 30001677 | ID=MS.gene51003.t1;Parent=MS.gene51003 |
| chr3.2 | exon | 30001677 | 30001728 | 30001677 | ID=MS.gene51003.t1.exon1;Parent=MS.gene51003.t1 |
| chr3.2 | CDS | 30001677 | 30001728 | 30001677 | ID=cds.MS.gene51003.t1;Parent=MS.gene51003.t1 |
| chr3.2 | exon | 30001939 | 30002351 | 30001939 | ID=MS.gene51003.t1.exon2;Parent=MS.gene51003.t1 |
| chr3.2 | CDS | 30001939 | 30002351 | 30001939 | ID=cds.MS.gene51003.t1;Parent=MS.gene51003.t1 |
| Gene Sequence |
| Protein sequence |
