AlfalfaGEDB | MS.gene51016

M. sativa cultivar XinJiangDaYe / MS.gene51016

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51016.t1	ABN09016.1	84.9	73	11	0	1	73	216	288	3.00E-30	140.6

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51016.t1	Q9MAA2	64.6	65	23	0	1	65	188	252	8.4e-21	100.5

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51016.t1	A2Q5Z6	84.9	73	11	0	1	73	216	288	2.2e-30	140.6

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049701	MS.gene51016	0.806662	7.38E-50	-1.69E-46
MS.gene054124	MS.gene51016	0.893252	7.47E-75	-1.69E-46
MS.gene056167	MS.gene51016	0.808997	2.35E-50	-1.69E-46
MS.gene056168	MS.gene51016	0.836095	1.14E-56	-1.69E-46
MS.gene060036	MS.gene51016	0.851049	1.14E-60	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51016.t1	MTR_7g113920	84.932	73	11	1	73	216	288	3.19e-43	142
MS.gene51016.t1	MTR_1g082360	64.384	73	26	1	73	184	256	1.58e-30	108
MS.gene51016.t1	MTR_5g010090	49.315	73	37	1	73	262	334	2.48e-18	77.0

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene51016.t1	AT3G05170	63.636	66	24	0	1	66	188	253	2.52e-25	95.5
MS.gene51016.t1	AT1G08940	61.404	57	22	0	2	58	192	248	8.14e-25	93.6

Find 20 sgRNAs with CRISPR-Local

Find 21 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TTTGAACTTCTCAACAATTT+TGG	0.154138	3.2:+30507103	MS.gene51016:CDS
TCCTAATGAGGTGGTTTAGA+TGG	0.351426	3.2:+30507068	None:intergenic
AGTTCCGTGTGATGCAGTTT+GGG	0.433577	3.2:+30507134	MS.gene51016:CDS
CAAATGAAGAACTGCTTAAG+TGG	0.436923	3.2:+30507191	MS.gene51016:CDS
GAGTTCCGTGTGATGCAGTT+TGG	0.441309	3.2:+30507133	MS.gene51016:CDS
TCAAGGGAGCCTTGCCGCCA+TGG	0.449335	3.2:-30507255	None:intergenic
CGTGTGATGCAGTTTGGGAC+TGG	0.458259	3.2:+30507139	MS.gene51016:CDS
TTCTGATCAGCTACCATATG+AGG	0.460428	3.2:-30507223	None:intergenic
ATATGGTAGCTGATCAGAAA+TGG	0.478679	3.2:+30507227	MS.gene51016:CDS
CAAGGGAGCCTTGCCGCCAT+GGG	0.494882	3.2:-30507254	None:intergenic
AAATGAAGAACTGCTTAAGT+GGG	0.557990	3.2:+30507192	MS.gene51016:CDS
GACTGGTGGAGAGTACATCT+TGG	0.576863	3.2:+30507156	MS.gene51016:CDS
GTGATGCAGTTTGGGACTGG+TGG	0.583634	3.2:+30507142	MS.gene51016:CDS
ATGGTGTGCCCATGGCGGCA+AGG	0.589094	3.2:+30507246	MS.gene51016:CDS
CAGTCCCAAACTGCATCACA+CGG	0.634212	3.2:-30507138	None:intergenic
GATCAGAAATGGTGTGCCCA+TGG	0.641634	3.2:+30507238	MS.gene51016:CDS
AATGAAGAACTGCTTAAGTG+GGG	0.655639	3.2:+30507193	MS.gene51016:CDS
AAAATGCTTCAAGGCAGTGG+GGG	0.655908	3.2:-30507289	None:intergenic
ATGAAGAACTGCTTAAGTGG+GGG	0.662094	3.2:+30507194	MS.gene51016:CDS
CAGAAATGGTGTGCCCATGG+CGG	0.769825	3.2:+30507241	MS.gene51016:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TTTGAACTTCTCAACAATTT+TGG	+	chr3.2:30507103-30507122	MS.gene51016:CDS	25.0%
	AAATGAAGAACTGCTTAAGT+GGG	+	chr3.2:30507192-30507211	MS.gene51016:CDS	30.0%
	AATGAAGAACTGCTTAAGTG+GGG	+	chr3.2:30507193-30507212	MS.gene51016:CDS	35.0%
	ATATGGTAGCTGATCAGAAA+TGG	+	chr3.2:30507227-30507246	MS.gene51016:CDS	35.0%
	CAAATGAAGAACTGCTTAAG+TGG	+	chr3.2:30507191-30507210	MS.gene51016:CDS	35.0%
	ATGAAGAACTGCTTAAGTGG+GGG	+	chr3.2:30507194-30507213	MS.gene51016:CDS	40.0%
	TTCTGATCAGCTACCATATG+AGG	-	chr3.2:30507226-30507245	None:intergenic	40.0%
	AGTTCCGTGTGATGCAGTTT+GGG	+	chr3.2:30507134-30507153	MS.gene51016:CDS	45.0%
	GGGGGACTTTGAACATTCAA+GGG	-	chr3.2:30507274-30507293	None:intergenic	45.0%
	TGGGGGACTTTGAACATTCA+AGG	-	chr3.2:30507275-30507294	None:intergenic	45.0%
	CAGTCCCAAACTGCATCACA+CGG	-	chr3.2:30507141-30507160	None:intergenic	50.0%
	GAGTTCCGTGTGATGCAGTT+TGG	+	chr3.2:30507133-30507152	MS.gene51016:CDS	50.0%
	GATCAGAAATGGTGTGCCCA+TGG	+	chr3.2:30507238-30507257	MS.gene51016:CDS	50.0%
!	GACTGGTGGAGAGTACATCT+TGG	+	chr3.2:30507156-30507175	MS.gene51016:CDS	50.0%
	CAGAAATGGTGTGCCCATGG+CGG	+	chr3.2:30507241-30507260	MS.gene51016:CDS	55.0%
!	CGTGTGATGCAGTTTGGGAC+TGG	+	chr3.2:30507139-30507158	MS.gene51016:CDS	55.0%
!	GTGATGCAGTTTGGGACTGG+TGG	+	chr3.2:30507142-30507161	MS.gene51016:CDS	55.0%
	GTGGGGGCTCTCTCCTCATA+TGG	+	chr3.2:30507210-30507229	MS.gene51016:CDS	60.0%
	ATGGTGTGCCCATGGCGGCA+AGG	+	chr3.2:30507246-30507265	MS.gene51016:CDS	65.0%
	CAAGGGAGCCTTGCCGCCAT+GGG	-	chr3.2:30507257-30507276	None:intergenic	65.0%
	TCAAGGGAGCCTTGCCGCCA+TGG	-	chr3.2:30507258-30507277	None:intergenic	65.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.2	gene	30507073	30507303	30507073	ID=MS.gene51016
chr3.2	mRNA	30507073	30507303	30507073	ID=MS.gene51016.t1;Parent=MS.gene51016
chr3.2	exon	30507073	30507303	30507073	ID=MS.gene51016.t1.exon1;Parent=MS.gene51016.t1
chr3.2	CDS	30507073	30507303	30507073	ID=cds.MS.gene51016.t1;Parent=MS.gene51016.t1

Gene Sequence	>MS.gene51016 ATGAGGTGGTTTAGATGGACAGTTGAACAATTTGAACTTCTCAACAATTTTGGAAGCTGTGAGTTCCGTGTGATGCAGTTTGGGACTGGTGGAGAGTACATCTTGGCAGTTCATCATACAAATGAAGAACTGCTTAAGTGGGGGCTCTCTCCTCATATGGTAGCTGATCAGAAATGGTGTGCCCATGGCGGCAAGGCTCCCTTGAATGTTCAAAGTCCCCCACTGCCTTGA
Protein sequence	>MS.gene51016.t1 MRWFRWTVEQFELLNNFGSCEFRVMQFGTGGEYILAVHHTNEELLKWGLSPHMVADQKWCAHGGKAPLNVQSPPLP