Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049701.t1 | RHN82442.1 | 91.2 | 170 | 12 | 2 | 1 | 168 | 72 | 240 | 2.90E-73 | 284.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049701.t1 | I3RZH9 | 91.2 | 170 | 12 | 2 | 1 | 168 | 1 | 169 | 2.1e-73 | 284.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049701 | MS.gene051710 | 0.823856 | 1.11E-53 | -1.69E-46 |
| MS.gene049701 | MS.gene058515 | 0.823626 | 1.25E-53 | -1.69E-46 |
| MS.gene049701 | MS.gene059276 | 0.816276 | 6.01E-52 | -1.69E-46 |
| MS.gene049701 | MS.gene09451 | 0.806252 | 9.00E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene09504 | 0.806109 | 9.65E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene26056 | 0.801115 | 1.05E-48 | -1.69E-46 |
| MS.gene049701 | MS.gene29253 | 0.813702 | 2.24E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene33462 | 0.821258 | 4.44E-53 | -1.69E-46 |
| MS.gene049701 | MS.gene34251 | 0.848466 | 6.02E-60 | -1.69E-46 |
| MS.gene049701 | MS.gene38011 | 0.828278 | 9.82E-55 | -1.69E-46 |
| MS.gene049701 | MS.gene38152 | 0.807179 | 5.73E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene38429 | 0.843083 | 1.74E-58 | -1.69E-46 |
| MS.gene049701 | MS.gene44743 | 0.808756 | 2.65E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene45794 | 0.831167 | 1.95E-55 | -1.69E-46 |
| MS.gene049701 | MS.gene48858 | 0.812495 | 4.12E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene51016 | 0.806662 | 7.38E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene59780 | 0.832295 | 1.03E-55 | -1.69E-46 |
| MS.gene049701 | MS.gene61742 | 0.807776 | 4.28E-50 | -1.69E-46 |
| MS.gene049701 | MS.gene66192 | 0.804064 | 2.59E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene66682 | 0.805126 | 1.55E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene67930 | 0.802596 | 5.20E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene69594 | 0.804485 | 2.11E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene74025 | 0.801667 | 8.08E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene76063 | 0.803122 | 4.05E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene76987 | 0.826378 | 2.80E-54 | -1.69E-46 |
| MS.gene049701 | MS.gene78647 | 0.814935 | 1.20E-51 | -1.69E-46 |
| MS.gene049701 | MS.gene79147 | 0.829543 | 4.86E-55 | -1.69E-46 |
| MS.gene049701 | MS.gene80853 | 0.800105 | 1.68E-48 | -1.69E-46 |
| MS.gene049701 | MS.gene82962 | 0.822581 | 2.19E-53 | -1.69E-46 |
| MS.gene049701 | MS.gene83166 | 0.804783 | 1.83E-49 | -1.69E-46 |
| MS.gene049701 | MS.gene84968 | 0.824377 | 8.34E-54 | -1.69E-46 |
| MS.gene049701 | MS.gene97797 | 0.823468 | 1.36E-53 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049701.t1 | MTR_1g111060 | 90.588 | 170 | 13 | 2 | 1 | 168 | 1 | 169 | 8.28e-95 | 273 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene049701.t1 | AT1G13360 | 48.538 | 171 | 72 | 6 | 6 | 168 | 8 | 170 | 1.01e-34 | 120 |
| MS.gene049701.t1 | AT1G13360 | 48.538 | 171 | 72 | 6 | 6 | 168 | 8 | 170 | 2.80e-34 | 119 |
| MS.gene049701.t1 | AT1G13360 | 48.538 | 171 | 72 | 6 | 6 | 168 | 8 | 170 | 7.48e-34 | 118 |
| MS.gene049701.t1 | AT3G25870 | 43.357 | 143 | 67 | 5 | 1 | 139 | 4 | 136 | 2.72e-15 | 69.7 |
Find 37 sgRNAs with CRISPR-Local
Find 38 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TATGATGAATCGTTTGATTT+AGG | 0.171666 | 3.3:-58283479 | MS.gene049701:CDS |
| TTTGATTTAGGAACTGGATT+TGG | 0.334138 | 3.3:-58283467 | MS.gene049701:CDS |
| GAATCGTTTGATTTAGGAAC+TGG | 0.338650 | 3.3:-58283473 | MS.gene049701:CDS |
| CTCCTTGTGACGTCAGATTC+CGG | 0.349448 | 3.3:-58283680 | MS.gene049701:CDS |
| TTCCGTTATGAAGAGTTTAC+AGG | 0.350599 | 3.3:-58283726 | MS.gene049701:CDS |
| CGTCTGGAATCGGTGAGTTA+TGG | 0.354406 | 3.3:-58283526 | MS.gene049701:CDS |
| GTCTGGAATCGGTGAGTTAT+GGG | 0.354798 | 3.3:-58283525 | MS.gene049701:CDS |
| GGAACTGGATTTGGAAGTTA+TGG | 0.360087 | 3.3:-58283458 | MS.gene049701:CDS |
| AGTTCGTCGTCGGAAGCTTC+AGG | 0.377726 | 3.3:+58283635 | None:intergenic |
| ACTGATTATGTTGCGTTTGA+TGG | 0.391469 | 3.3:-58283416 | MS.gene049701:CDS |
| TTGTCTGACTCGTCTGGAAT+CGG | 0.424690 | 3.3:-58283536 | MS.gene049701:CDS |
| TTCTTATCTTCCTTCACCGT+CGG | 0.482664 | 3.3:+58283584 | None:intergenic |
| GTTGCGTTTGATGGGATTTG+AGG | 0.485104 | 3.3:-58283407 | None:intergenic |
| AGATCTTGTGTTGAAGGGGT+TGG | 0.487761 | 3.3:+58283752 | None:intergenic |
| AATCAAGATCTTGTGTTGAA+GGG | 0.506132 | 3.3:+58283747 | None:intergenic |
| GATTCCGGCGAGTCTCAGAT+TGG | 0.511861 | 3.3:-58283665 | MS.gene049701:CDS |
| CTGATTATGTTGCGTTTGAT+GGG | 0.516937 | 3.3:-58283415 | MS.gene049701:CDS |
| CTAGTGTCAATAACAAGAAA+CGG | 0.526588 | 3.3:-58283868 | MS.gene049701:CDS |
| GAATCAAGATCTTGTGTTGA+AGG | 0.534956 | 3.3:+58283746 | None:intergenic |
| CGCCGGAATCTGACGTCACA+AGG | 0.537931 | 3.3:+58283678 | None:intergenic |
| CGAACTCGGTCTTCCACCGC+CGG | 0.539234 | 3.3:-58283618 | MS.gene049701:CDS |
| TTGTGTTGAAGGGGTTGGAT+CGG | 0.547196 | 3.3:+58283757 | None:intergenic |
| ATATGAATATCCGACGGTGA+AGG | 0.549802 | 3.3:-58283594 | MS.gene049701:CDS |
| GGATATTCATATCCCGGCGG+TGG | 0.565893 | 3.3:+58283605 | None:intergenic |
| ATTCCGGCGAGTCTCAGATT+GGG | 0.568497 | 3.3:-58283664 | MS.gene049701:CDS |
| CAGACGAGTCAGACAAAACT+CGG | 0.578186 | 3.3:+58283543 | None:intergenic |
| ATCCTGTAAACTCTTCATAA+CGG | 0.581375 | 3.3:+58283724 | None:intergenic |
| GTACCCAATCTGAGACTCGC+CGG | 0.584365 | 3.3:+58283661 | None:intergenic |
| ATCAAGATCTTGTGTTGAAG+GGG | 0.584581 | 3.3:+58283748 | None:intergenic |
| ACCGTCGGATATTCATATCC+CGG | 0.593623 | 3.3:+58283599 | None:intergenic |
| GAAGCTTCCGACGACGAACT+CGG | 0.594739 | 3.3:-58283632 | MS.gene049701:CDS |
| TGGAAGACCGAGTTCGTCGT+CGG | 0.623224 | 3.3:+58283625 | None:intergenic |
| GAACTCGGTCTTCCACCGCC+GGG | 0.647667 | 3.3:-58283617 | MS.gene049701:CDS |
| CGTCGTCGGAAGCTTCAGGA+AGG | 0.650984 | 3.3:+58283639 | None:intergenic |
| GTCAGAAGTTGAATCGTCCG+AGG | 0.668675 | 3.3:-58283822 | MS.gene049701:CDS |
| GCCGGGATATGAATATCCGA+CGG | 0.699473 | 3.3:-58283600 | MS.gene049701:CDS |
| GTCGGATATTCATATCCCGG+CGG | 0.769927 | 3.3:+58283602 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATGATGAATCGTTTGATTT+AGG | - | chr3.3:58283823-58283842 | MS.gene049701:CDS | 25.0% |
| AATCAAGATCTTGTGTTGAA+GGG | + | chr3.3:58283558-58283577 | None:intergenic | 30.0% | |
| ATCCTGTAAACTCTTCATAA+CGG | + | chr3.3:58283581-58283600 | None:intergenic | 30.0% | |
| CTAGTGTCAATAACAAGAAA+CGG | - | chr3.3:58283434-58283453 | MS.gene049701:CDS | 30.0% | |
| ! | TTTGATTTAGGAACTGGATT+TGG | - | chr3.3:58283835-58283854 | MS.gene049701:CDS | 30.0% |
| ACTGATTATGTTGCGTTTGA+TGG | - | chr3.3:58283886-58283905 | MS.gene049701:CDS | 35.0% | |
| ATCAAGATCTTGTGTTGAAG+GGG | + | chr3.3:58283557-58283576 | None:intergenic | 35.0% | |
| CTGATTATGTTGCGTTTGAT+GGG | - | chr3.3:58283887-58283906 | MS.gene049701:CDS | 35.0% | |
| GAATCAAGATCTTGTGTTGA+AGG | + | chr3.3:58283559-58283578 | None:intergenic | 35.0% | |
| TTCCGTTATGAAGAGTTTAC+AGG | - | chr3.3:58283576-58283595 | MS.gene049701:CDS | 35.0% | |
| ! | GAATCGTTTGATTTAGGAAC+TGG | - | chr3.3:58283829-58283848 | MS.gene049701:CDS | 35.0% |
| ATATGAATATCCGACGGTGA+AGG | - | chr3.3:58283708-58283727 | MS.gene049701:CDS | 40.0% | |
| GGAACTGGATTTGGAAGTTA+TGG | - | chr3.3:58283844-58283863 | MS.gene049701:CDS | 40.0% | |
| TTCTTATCTTCCTTCACCGT+CGG | + | chr3.3:58283721-58283740 | None:intergenic | 40.0% | |
| !! | ATCTCTGATTCGTTTTGCCT+CGG | + | chr3.3:58283500-58283519 | None:intergenic | 40.0% |
| ACCGTCGGATATTCATATCC+CGG | + | chr3.3:58283706-58283725 | None:intergenic | 45.0% | |
| AGATCTTGTGTTGAAGGGGT+TGG | + | chr3.3:58283553-58283572 | None:intergenic | 45.0% | |
| CAGACGAGTCAGACAAAACT+CGG | + | chr3.3:58283762-58283781 | None:intergenic | 45.0% | |
| TTGTGTTGAAGGGGTTGGAT+CGG | + | chr3.3:58283548-58283567 | None:intergenic | 45.0% | |
| ! | GTCTGGAATCGGTGAGTTAT+GGG | - | chr3.3:58283777-58283796 | MS.gene049701:CDS | 45.0% |
| ! | TTGTCTGACTCGTCTGGAAT+CGG | - | chr3.3:58283766-58283785 | MS.gene049701:CDS | 45.0% |
| ATTCCGGCGAGTCTCAGATT+GGG | - | chr3.3:58283638-58283657 | MS.gene049701:CDS | 50.0% | |
| CTCCTTGTGACGTCAGATTC+CGG | - | chr3.3:58283622-58283641 | MS.gene049701:CDS | 50.0% | |
| GCCGGGATATGAATATCCGA+CGG | - | chr3.3:58283702-58283721 | MS.gene049701:CDS | 50.0% | |
| GTCAGAAGTTGAATCGTCCG+AGG | - | chr3.3:58283480-58283499 | MS.gene049701:CDS | 50.0% | |
| GTCGGATATTCATATCCCGG+CGG | + | chr3.3:58283703-58283722 | None:intergenic | 50.0% | |
| ! | CGTCTGGAATCGGTGAGTTA+TGG | - | chr3.3:58283776-58283795 | MS.gene049701:CDS | 50.0% |
| !! | CGAGTTTTGTCTGACTCGTC+TGG | - | chr3.3:58283760-58283779 | MS.gene049701:CDS | 50.0% |
| AGTTCGTCGTCGGAAGCTTC+AGG | + | chr3.3:58283670-58283689 | None:intergenic | 55.0% | |
| GAAGCTTCCGACGACGAACT+CGG | - | chr3.3:58283670-58283689 | MS.gene049701:CDS | 55.0% | |
| GATTCCGGCGAGTCTCAGAT+TGG | - | chr3.3:58283637-58283656 | MS.gene049701:CDS | 55.0% | |
| GGATATTCATATCCCGGCGG+TGG | + | chr3.3:58283700-58283719 | None:intergenic | 55.0% | |
| TGGAAGACCGAGTTCGTCGT+CGG | + | chr3.3:58283680-58283699 | None:intergenic | 55.0% | |
| ! | GTACCCAATCTGAGACTCGC+CGG | + | chr3.3:58283644-58283663 | None:intergenic | 55.0% |
| CGCCGGAATCTGACGTCACA+AGG | + | chr3.3:58283627-58283646 | None:intergenic | 60.0% | |
| CGTCGTCGGAAGCTTCAGGA+AGG | + | chr3.3:58283666-58283685 | None:intergenic | 60.0% | |
| CGAACTCGGTCTTCCACCGC+CGG | - | chr3.3:58283684-58283703 | MS.gene049701:CDS | 65.0% | |
| GAACTCGGTCTTCCACCGCC+GGG | - | chr3.3:58283685-58283704 | MS.gene049701:CDS | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 58283409 | 58283915 | 58283409 | ID=MS.gene049701 |
| chr3.3 | mRNA | 58283409 | 58283915 | 58283409 | ID=MS.gene049701.t1;Parent=MS.gene049701 |
| chr3.3 | exon | 58283409 | 58283915 | 58283409 | ID=MS.gene049701.t1.exon1;Parent=MS.gene049701.t1 |
| chr3.3 | CDS | 58283409 | 58283915 | 58283409 | ID=cds.MS.gene049701.t1;Parent=MS.gene049701.t1 |
| Gene Sequence |
| Protein sequence |
