Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51113.t1 | XP_003616743.2 | 98.7 | 155 | 2 | 0 | 1 | 155 | 25 | 179 | 7.10E-74 | 286.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51113.t1 | G7K7C1 | 98.7 | 155 | 2 | 0 | 1 | 155 | 25 | 179 | 5.1e-74 | 286.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054473 | MS.gene51113 | 0.802501 | 5.44E-49 | -1.69E-46 |
| MS.gene055277 | MS.gene51113 | 0.802915 | 4.47E-49 | -1.69E-46 |
| MS.gene055428 | MS.gene51113 | 0.80824 | 3.41E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51113.t1 | MTR_5g083810 | 98.710 | 155 | 2 | 0 | 1 | 155 | 25 | 179 | 4.48e-110 | 310 |
| MS.gene51113.t1 | MTR_3g466150 | 46.835 | 158 | 82 | 1 | 1 | 156 | 1 | 158 | 5.24e-46 | 147 |
| MS.gene51113.t1 | MTR_7g072470 | 58.537 | 123 | 50 | 1 | 27 | 149 | 6 | 127 | 3.67e-43 | 139 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51113.t1 | AT2G40435 | 64.103 | 156 | 54 | 2 | 1 | 155 | 1 | 155 | 5.38e-68 | 203 |
| MS.gene51113.t1 | AT3G56220 | 57.419 | 155 | 63 | 3 | 1 | 153 | 1 | 154 | 1.29e-57 | 177 |
| MS.gene51113.t1 | AT2G40435 | 64.341 | 129 | 45 | 1 | 1 | 128 | 1 | 129 | 5.97e-56 | 172 |
| MS.gene51113.t1 | AT2G40435 | 65.354 | 127 | 43 | 1 | 1 | 126 | 1 | 127 | 6.67e-56 | 172 |
| MS.gene51113.t1 | AT3G56220 | 57.364 | 129 | 52 | 3 | 1 | 127 | 1 | 128 | 3.14e-44 | 142 |
| MS.gene51113.t1 | AT1G29270 | 35.156 | 128 | 75 | 5 | 1 | 123 | 1 | 125 | 5.42e-11 | 57.8 |
Find 22 sgRNAs with CRISPR-Local
Find 100 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTCAATTACCATAGGCAAGT+TGG | 0.394555 | 5.1:+65994801 | None:intergenic |
| TCAATTACCATAGGCAAGTT+GGG | 0.413845 | 5.1:+65994802 | None:intergenic |
| TACAGTGGAAACCCTAGAAA+AGG | 0.442701 | 5.1:-65994235 | MS.gene51113:CDS |
| GCTTACTGTGCTGGAAGCTA+GGG | 0.457026 | 5.1:-65994133 | MS.gene51113:CDS |
| GGTTGCAATTTACACTTGAT+CGG | 0.468244 | 5.1:+65993555 | None:intergenic |
| TATTGAAAATCTAAAGGAAA+AGG | 0.484456 | 5.1:-65994878 | MS.gene51113:CDS |
| AGAGATGAGGCTTACTGTGC+TGG | 0.485736 | 5.1:-65994142 | MS.gene51113:CDS |
| GGCTTACTGTGCTGGAAGCT+AGG | 0.491888 | 5.1:-65994134 | MS.gene51113:CDS |
| CCACAATCCCAACTTGCCTA+TGG | 0.504234 | 5.1:-65994809 | MS.gene51113:intron |
| AAAGTATATTGAAAATCTAA+AGG | 0.516255 | 5.1:-65994884 | MS.gene51113:CDS |
| AGTAAGCCTCATCTCTTCAA+TGG | 0.521158 | 5.1:+65994149 | None:intergenic |
| CCATAGGCAAGTTGGGATTG+TGG | 0.527764 | 5.1:+65994809 | None:intergenic |
| ACAGTGGAAACCCTAGAAAA+GGG | 0.541907 | 5.1:-65994234 | MS.gene51113:CDS |
| TTACCTCTCCTCCAACAGCT+TGG | 0.548657 | 5.1:+65994085 | None:intergenic |
| TTTCCAAGCTGTTGGAGGAG+AGG | 0.549162 | 5.1:-65994088 | MS.gene51113:intron |
| TTGAAGCCATTGAAGAGATG+AGG | 0.583640 | 5.1:-65994155 | MS.gene51113:CDS |
| TGATGCATCGATTACGATCG+AGG | 0.609202 | 5.1:+65994906 | None:intergenic |
| GTATTGAAACAAGCATACCT+TGG | 0.629414 | 5.1:+65994178 | None:intergenic |
| TGGACTTGTGCAGGTTACAG+TGG | 0.658893 | 5.1:-65994250 | MS.gene51113:intron |
| ACAAGCTGTGAAACAAGCCG+TGG | 0.676305 | 5.1:-65993608 | MS.gene51113:CDS |
| CAAGCTGTGAAACAAGCCGT+GGG | 0.688309 | 5.1:-65993607 | MS.gene51113:CDS |
| CTTATGTAGAATGAAGAACA+AGG | 0.699994 | 5.1:-65993646 | MS.gene51113:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAGACTATATATTATTATTA+GGG | + | chr5.1:65995358-65995377 | None:intergenic | 10.0% |
| !! | ATTAATTCCAATAATAAAAA+TGG | + | chr5.1:65993790-65993809 | None:intergenic | 10.0% |
| !! | AAAGTATATTGAAAATCTAA+AGG | - | chr5.1:65994249-65994268 | MS.gene51113:intron | 15.0% |
| !! | AACTTATAAACAATTAAACA+TGG | - | chr5.1:65994557-65994576 | MS.gene51113:intron | 15.0% |
| !! | GAAGACTATATATTATTATT+AGG | + | chr5.1:65995359-65995378 | None:intergenic | 15.0% |
| !! | TAATAAAAAAATAGCAAACT+TGG | + | chr5.1:65993817-65993836 | None:intergenic | 15.0% |
| !!! | AATTCACTTTTTTTTTTCTT+GGG | - | chr5.1:65994728-65994747 | MS.gene51113:intron | 15.0% |
| !!! | GAATATTCTTTTATAGAATT+GGG | + | chr5.1:65993730-65993749 | None:intergenic | 15.0% |
| !!! | TAATTCACTTTTTTTTTTCT+TGG | - | chr5.1:65994727-65994746 | MS.gene51113:intron | 15.0% |
| !!! | TGAATATTCTTTTATAGAAT+TGG | + | chr5.1:65993731-65993750 | None:intergenic | 15.0% |
| !! | ATATAAAAACGTTACTCATT+TGG | - | chr5.1:65995247-65995266 | MS.gene51113:intron | 20.0% |
| !! | TATAAAAACGTTACTCATTT+GGG | - | chr5.1:65995248-65995267 | MS.gene51113:intron | 20.0% |
| !! | TATTGAAAATCTAAAGGAAA+AGG | - | chr5.1:65994255-65994274 | MS.gene51113:intron | 20.0% |
| !! | TTATTATTAGGGTTAAGATT+AGG | + | chr5.1:65995347-65995366 | None:intergenic | 20.0% |
| !! | TTTCATTGAGAATATATTGA+TGG | + | chr5.1:65993704-65993723 | None:intergenic | 20.0% |
| !! | TTTCTAATACTTTCTTCTTA+TGG | - | chr5.1:65994170-65994189 | MS.gene51113:CDS | 20.0% |
| !!! | GAAATTTGAAATGGATTTTA+TGG | - | chr5.1:65994859-65994878 | MS.gene51113:CDS | 20.0% |
| !!! | GAGTAACGTTTTTATATAAA+GGG | + | chr5.1:65995244-65995263 | None:intergenic | 20.0% |
| !!! | TGAGTAACGTTTTTATATAA+AGG | + | chr5.1:65995245-65995264 | None:intergenic | 20.0% |
| ! | AATGGAAAGAAAAGAAAGAT+AGG | + | chr5.1:65993671-65993690 | None:intergenic | 25.0% |
| ! | ACAAAAATATTGACAAGTGA+AGG | + | chr5.1:65995207-65995226 | None:intergenic | 25.0% |
| ! | ATAAAAACGTTACTCATTTG+GGG | - | chr5.1:65995249-65995268 | MS.gene51113:intron | 25.0% |
| ! | GAGAAATTGTTTACAATAAC+TGG | + | chr5.1:65994765-65994784 | None:intergenic | 25.0% |
| ! | GCAGAAAGAATGAATTAATA+AGG | + | chr5.1:65993891-65993910 | None:intergenic | 25.0% |
| ! | GTACATTGAATCATTGATAT+TGG | - | chr5.1:65995298-65995317 | MS.gene51113:intron | 25.0% |
| ! | TCTTCATTTAATTGTCTACT+AGG | - | chr5.1:65995374-65995393 | MS.gene51113:intron | 25.0% |
| ! | TGCAAGTATGAAATTTGAAA+TGG | - | chr5.1:65994850-65994869 | MS.gene51113:CDS | 25.0% |
| !! | TATAAGAAATCCCTTTTCTA+GGG | + | chr5.1:65994912-65994931 | None:intergenic | 25.0% |
| !! | TATCTTTCTTTTCTTTCCAT+TGG | - | chr5.1:65993670-65993689 | MS.gene51113:intron | 25.0% |
| !! | TTATAAGAAATCCCTTTTCT+AGG | + | chr5.1:65994913-65994932 | None:intergenic | 25.0% |
| !!! | GTCTCTTTGAATTGTTTTAT+TGG | + | chr5.1:65994649-65994668 | None:intergenic | 25.0% |
| !!! | TTTGAAATGGATTTTATGGA+TGG | - | chr5.1:65994863-65994882 | MS.gene51113:CDS | 25.0% |
| !!! | TTTGGGTCCATTTTTATTAT+TGG | - | chr5.1:65993780-65993799 | MS.gene51113:intron | 25.0% |
| !!! | TTTTTTAGGATAGATAGACA+AGG | + | chr5.1:65994675-65994694 | None:intergenic | 25.0% |
| AAGATTAGGCACCATATATA+TGG | + | chr5.1:65995333-65995352 | None:intergenic | 30.0% | |
| AATAAAAATGGACCCAAATC+AGG | + | chr5.1:65993778-65993797 | None:intergenic | 30.0% | |
| AATTCTGCTGCAAGTTATTT+AGG | + | chr5.1:65994708-65994727 | None:intergenic | 30.0% | |
| AGGATACACATAAACTACTA+TGG | + | chr5.1:65994095-65994114 | None:intergenic | 30.0% | |
| ATTCTGCTGCAAGTTATTTA+GGG | + | chr5.1:65994707-65994726 | None:intergenic | 30.0% | |
| CACATAGCTGATAAGAATAT+AGG | + | chr5.1:65994121-65994140 | None:intergenic | 30.0% | |
| CTATATTCTTATCAGCTATG+TGG | - | chr5.1:65994119-65994138 | MS.gene51113:CDS | 30.0% | |
| CTTATGTAGAATGAAGAACA+AGG | - | chr5.1:65995487-65995506 | MS.gene51113:intron | 30.0% | |
| GCTGATAAGAATATAGGATA+AGG | + | chr5.1:65994115-65994134 | None:intergenic | 30.0% | |
| TATACTCTTGTTCCTGATTT+GGG | - | chr5.1:65993763-65993782 | MS.gene51113:intron | 30.0% | |
| TATGTGGAGCTTATTTGATA+TGG | - | chr5.1:65994135-65994154 | MS.gene51113:CDS | 30.0% | |
| TGGGACAAGCAATAAAAAAT+TGG | - | chr5.1:65995545-65995564 | MS.gene51113:CDS | 30.0% | |
| ! | CTTCTTATGGTTTTTATGCA+TGG | - | chr5.1:65994183-65994202 | MS.gene51113:CDS | 30.0% |
| ! | GTACTTTTCTCAATTACCAT+AGG | + | chr5.1:65994343-65994362 | None:intergenic | 30.0% |
| ! | TATGGTTTTTATGCATGGTT+TGG | - | chr5.1:65994188-65994207 | MS.gene51113:CDS | 30.0% |
| !! | AATTTTTGTTTCCAAGCTGT+TGG | - | chr5.1:65995037-65995056 | MS.gene51113:intron | 30.0% |
| !! | CTGCTTTTGGATAAACAATT+TGG | - | chr5.1:65994439-65994458 | MS.gene51113:intron | 30.0% |
| !!! | ATTTTTTATTGCTTGTCCCA+CGG | + | chr5.1:65995545-65995564 | None:intergenic | 30.0% |
| !!! | TAGGGTCAAGACTTTTTTTT+AGG | + | chr5.1:65994689-65994708 | None:intergenic | 30.0% |
| AGATAGGAGATAGAAAATGC+AGG | + | chr5.1:65993655-65993674 | None:intergenic | 35.0% | |
| ATTGATGGAGAGAAATCCAA+TGG | + | chr5.1:65993689-65993708 | None:intergenic | 35.0% | |
| CTATACTCTTGTTCCTGATT+TGG | - | chr5.1:65993762-65993781 | MS.gene51113:intron | 35.0% | |
| CTCACAAGTGTAATGTTAGT+AGG | - | chr5.1:65994613-65994632 | MS.gene51113:intron | 35.0% | |
| GATAGGAGATAGAAAATGCA+GGG | + | chr5.1:65993654-65993673 | None:intergenic | 35.0% | |
| GGTCATGCAATCCATATATA+TGG | - | chr5.1:65995319-65995338 | MS.gene51113:intron | 35.0% | |
| GTATTGAAACAAGCATACCT+TGG | + | chr5.1:65994958-65994977 | None:intergenic | 35.0% | |
| TCAATTACCATAGGCAAGTT+GGG | + | chr5.1:65994334-65994353 | None:intergenic | 35.0% | |
| TCGTTATTGATGAACTTAGC+TGG | + | chr5.1:65995408-65995427 | None:intergenic | 35.0% | |
| TGATTCAATGTACAGCAACT+CGG | + | chr5.1:65995291-65995310 | None:intergenic | 35.0% | |
| TTAGGATAGATAGACAAGGA+TGG | + | chr5.1:65994671-65994690 | None:intergenic | 35.0% | |
| TTGTTAATATCGCGAGCTAA+TGG | + | chr5.1:65994592-65994611 | None:intergenic | 35.0% | |
| TTTGAACCTAGATATGCATG+TGG | + | chr5.1:65993946-65993965 | None:intergenic | 35.0% | |
| ! | GATCAGAATTTGACTGCTTT+TGG | - | chr5.1:65994426-65994445 | MS.gene51113:intron | 35.0% |
| ! | GGATGAGAGCATAAAAGTAT+AGG | + | chr5.1:65993925-65993944 | None:intergenic | 35.0% |
| !! | AGAATGACGGAAAGTTTTTG+TGG | - | chr5.1:65995464-65995483 | MS.gene51113:intron | 35.0% |
| ACAGTGGAAACCCTAGAAAA+GGG | - | chr5.1:65994899-65994918 | MS.gene51113:CDS | 40.0% | |
| AGTAAGCCTCATCTCTTCAA+TGG | + | chr5.1:65994987-65995006 | None:intergenic | 40.0% | |
| CTCAATTACCATAGGCAAGT+TGG | + | chr5.1:65994335-65994354 | None:intergenic | 40.0% | |
| TACAGTGGAAACCCTAGAAA+AGG | - | chr5.1:65994898-65994917 | MS.gene51113:CDS | 40.0% | |
| TCATAAGCCCTTCATCAAGA+CGG | + | chr5.1:65994522-65994541 | None:intergenic | 40.0% | |
| TCGTTAACCGTCTTGATGAA+GGG | - | chr5.1:65994512-65994531 | MS.gene51113:intron | 40.0% | |
| TCTCATCCACATGCATATCT+AGG | - | chr5.1:65993937-65993956 | MS.gene51113:intron | 40.0% | |
| TTCGTTAACCGTCTTGATGA+AGG | - | chr5.1:65994511-65994530 | MS.gene51113:intron | 40.0% | |
| TTGAAGCCATTGAAGAGATG+AGG | - | chr5.1:65994978-65994997 | MS.gene51113:intron | 40.0% | |
| ! | ATTTGGGGTTCTCGAGAAAA+GGG | - | chr5.1:65995264-65995283 | MS.gene51113:intron | 40.0% |
| ! | GAGCATAAAAGTATAGGCAG+TGG | + | chr5.1:65993919-65993938 | None:intergenic | 40.0% |
| ! | TTTTCTGCAAAAAGCAGCCA+AGG | - | chr5.1:65994938-65994957 | MS.gene51113:intron | 40.0% |
| !! | TTTTATGGATGGACTTGTGC+AGG | - | chr5.1:65994874-65994893 | MS.gene51113:CDS | 40.0% |
| !! | TTTTGTTTCCAAGCTGTTGG+AGG | - | chr5.1:65995040-65995059 | MS.gene51113:intron | 40.0% |
| CCCATCATGCATGAGAATGA+CGG | - | chr5.1:65995451-65995470 | MS.gene51113:intron | 45.0% | |
| CCGTCATTCTCATGCATGAT+GGG | + | chr5.1:65995454-65995473 | None:intergenic | 45.0% | |
| TCCGTCATTCTCATGCATGA+TGG | + | chr5.1:65995455-65995474 | None:intergenic | 45.0% | |
| ! | CATTTGGGGTTCTCGAGAAA+AGG | - | chr5.1:65995263-65995282 | MS.gene51113:intron | 45.0% |
| !! | TGATGCATCGATTACGATCG+AGG | + | chr5.1:65994230-65994249 | None:intergenic | 45.0% |
| !! | CTATAAAAAATTATATAAAT+GGG | + | chr5.1:65994371-65994390 | None:intergenic | 5.0% |
| !! | TCTATAAAAAATTATATAAA+TGG | + | chr5.1:65994372-65994391 | None:intergenic | 5.0% |
| ACAAGCTGTGAAACAAGCCG+TGG | - | chr5.1:65995525-65995544 | MS.gene51113:CDS | 50.0% | |
| AGAGATGAGGCTTACTGTGC+TGG | - | chr5.1:65994991-65995010 | MS.gene51113:intron | 50.0% | |
| CAAGCTGTGAAACAAGCCGT+GGG | - | chr5.1:65995526-65995545 | MS.gene51113:CDS | 50.0% | |
| CCACAATCCCAACTTGCCTA+TGG | - | chr5.1:65994324-65994343 | MS.gene51113:intron | 50.0% | |
| GCTTACTGTGCTGGAAGCTA+GGG | - | chr5.1:65995000-65995019 | MS.gene51113:intron | 50.0% | |
| TGGACTTGTGCAGGTTACAG+TGG | - | chr5.1:65994883-65994902 | MS.gene51113:CDS | 50.0% | |
| TTACCTCTCCTCCAACAGCT+TGG | + | chr5.1:65995051-65995070 | None:intergenic | 50.0% | |
| TTTCCAAGCTGTTGGAGGAG+AGG | - | chr5.1:65995045-65995064 | MS.gene51113:intron | 50.0% | |
| ! | CCATAGGCAAGTTGGGATTG+TGG | + | chr5.1:65994327-65994346 | None:intergenic | 50.0% |
| GGCTTACTGTGCTGGAAGCT+AGG | - | chr5.1:65994999-65995018 | MS.gene51113:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 65993564 | 65995591 | 65993564 | ID=MS.gene51113 |
| chr5.1 | mRNA | 65993564 | 65995591 | 65993564 | ID=MS.gene51113.t1;Parent=MS.gene51113 |
| chr5.1 | exon | 65995514 | 65995591 | 65995514 | ID=MS.gene51113.t1.exon1;Parent=MS.gene51113.t1 |
| chr5.1 | CDS | 65995514 | 65995591 | 65995514 | ID=cds.MS.gene51113.t1;Parent=MS.gene51113.t1 |
| chr5.1 | exon | 65994810 | 65994938 | 65994810 | ID=MS.gene51113.t1.exon2;Parent=MS.gene51113.t1 |
| chr5.1 | CDS | 65994810 | 65994938 | 65994810 | ID=cds.MS.gene51113.t1;Parent=MS.gene51113.t1 |
| chr5.1 | exon | 65994089 | 65994259 | 65994089 | ID=MS.gene51113.t1.exon3;Parent=MS.gene51113.t1 |
| chr5.1 | CDS | 65994089 | 65994259 | 65994089 | ID=cds.MS.gene51113.t1;Parent=MS.gene51113.t1 |
| chr5.1 | exon | 65993564 | 65993659 | 65993564 | ID=MS.gene51113.t1.exon4;Parent=MS.gene51113.t1 |
| chr5.1 | CDS | 65993564 | 65993659 | 65993564 | ID=cds.MS.gene51113.t1;Parent=MS.gene51113.t1 |
| Gene Sequence |
| Protein sequence |
