AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene51421

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score

Find 49 sgRNAs with CRISPR-Local

Find 154 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCCTTTGCCTGTGGTGAAAT+TGG 0.182813 2.2:+54471822 None:intergenic
TTCATCTTCGATTGGTTCTA+TGG 0.267772 2.2:-54474174 MS.gene51421:CDS
ACAAAACCAACCCATTCTTT+TGG 0.292095 2.2:-54474229 MS.gene51421:CDS
ATCAAATTGCTCGTAGAGTT+TGG 0.294302 2.2:-54473870 MS.gene51421:CDS
AATGTTCCATTTCTTATTCT+AGG 0.294978 2.2:-54471907 MS.gene51421:CDS
CTTCGGAGGAATTGAGTATT+GGG 0.328437 2.2:-54473927 MS.gene51421:CDS
TTCTTGCATCCCTTGGTCTC+TGG 0.356031 2.2:-54474149 MS.gene51421:CDS
TATGGCATTCTTGCATCCCT+TGG 0.385266 2.2:-54474156 MS.gene51421:CDS
TCGTCTTCTGAAGCTGCATA+TGG 0.388503 2.2:+54471868 None:intergenic
CAACCATGTTCATCTTCGAT+TGG 0.422638 2.2:-54474182 MS.gene51421:CDS
GAAGCCAAGATTCTCTTCCT+TGG 0.425299 2.2:-54474120 MS.gene51421:CDS
GAGATCTAAACCCAAAAGAA+TGG 0.455176 2.2:+54474218 None:intergenic
TTCCTTGGTCTTGATAATGC+TGG 0.455864 2.2:-54474105 MS.gene51421:CDS
TTGTTTCCTAGAATAAGAAA+TGG 0.457150 2.2:+54471901 None:intergenic
ACTTCGGAGGAATTGAGTAT+TGG 0.465946 2.2:-54473928 MS.gene51421:CDS
CATCTTTGAGCATATGAAGA+AGG 0.466211 2.2:+54474073 None:intergenic
ATTGTCCGCAAGATGGGATA+TGG 0.471519 2.2:-54471745 MS.gene51421:CDS
TTCCTCCGAAGTAGGATGTT+GGG 0.479835 2.2:+54473939 None:intergenic
TGCAGCATTGTCCGCAAGAT+GGG 0.482538 2.2:-54471751 MS.gene51421:CDS
TTGGAAAGATTATTATGCTA+AGG 0.487117 2.2:-54473851 MS.gene51421:intron
AAGACCAAGGAAGAGAATCT+TGG 0.490940 2.2:+54474116 None:intergenic
AGATCTAAACCCAAAAGAAT+GGG 0.503502 2.2:+54474219 None:intergenic
TGATATATTGTGAAAGCCAC+TGG 0.515926 2.2:+54471713 None:intergenic
ACCAATTTCACCACAGGCAA+AGG 0.519780 2.2:-54471823 MS.gene51421:CDS
CGTCTTCTGAAGCTGCATAT+GGG 0.521363 2.2:+54471869 None:intergenic
GTGCAGCATTGTCCGCAAGA+TGG 0.523551 2.2:-54471752 MS.gene51421:CDS
TGCCTGTGGTGAAATTGGTT+AGG 0.535024 2.2:+54471827 None:intergenic
GAGGTTTGCAGAATCTAAGA+AGG 0.538939 2.2:-54471965 MS.gene51421:CDS
ATACTCAATTCCTCCGAAGT+AGG 0.542870 2.2:+54473931 None:intergenic
ATTCCTCCGAAGTAGGATGT+TGG 0.553310 2.2:+54473938 None:intergenic
ATTGACCTTGCCTTTGCCTG+TGG 0.559718 2.2:+54471813 None:intergenic
CGCAAGATGGGATATGGCGA+AGG 0.565211 2.2:-54471739 MS.gene51421:CDS
CTAAACCCAAAAGAATGGGT+TGG 0.570954 2.2:+54474223 None:intergenic
TCTTCATATGCTCAAAGATG+AGG 0.580955 2.2:-54474070 MS.gene51421:intron
ATCTTTGAGCATATGAAGAA+GGG 0.582029 2.2:+54474074 None:intergenic
CTTTGTCGTAAGCATCGACT+AGG 0.589660 2.2:+54471989 None:intergenic
GGCCTAACCAATTTCACCAC+AGG 0.595827 2.2:-54471829 MS.gene51421:CDS
CTGCACATGAACACTTCAAG+AGG 0.596703 2.2:+54471769 None:intergenic
TCGATGCTTACGACAAAGAG+AGG 0.612300 2.2:-54471984 MS.gene51421:CDS
ACCGACCCAACATCCTACTT+CGG 0.614242 2.2:-54473944 MS.gene51421:CDS
TTCCAGCATTATCAAGACCA+AGG 0.615002 2.2:+54474103 None:intergenic
GACGAGCTACGATATCATCT+CGG 0.618823 2.2:-54471850 MS.gene51421:CDS
TCCGAAGTAGGATGTTGGGT+CGG 0.619899 2.2:+54473943 None:intergenic
CACTTCAAGAGGTCGAACGT+TGG 0.639582 2.2:+54471780 None:intergenic
TTTCACCACAGGCAAAGGCA+AGG 0.642687 2.2:-54471818 MS.gene51421:CDS
CTTCGCCATATCCCATCTTG+CGG 0.645914 2.2:+54471740 None:intergenic
GAACCAATCGAAGATGAACA+TGG 0.652038 2.2:+54474179 None:intergenic
GACCCAACATCCTACTTCGG+AGG 0.659761 2.2:-54473941 MS.gene51421:CDS
GATATGGCGAAGGCTTCCAG+TGG 0.701402 2.2:-54471729 MS.gene51421:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATATAATGATCTATCT+AGG - chr2.2:54472560-54472579 MS.gene51421:intron 15.0%
!! ATAGATTATATCTGTATAAA+AGG - chr2.2:54473363-54473382 MS.gene51421:intron 15.0%
!!! AAACTTCAAATTTTTGTTAT+TGG + chr2.2:54473749-54473768 None:intergenic 15.0%
!!! ACAAAAATTTGAAGTTTTTT+TGG - chr2.2:54473752-54473771 MS.gene51421:intron 15.0%
!!! ATTTCTTGATTATCATTTTT+TGG - chr2.2:54472140-54472159 MS.gene51421:intron 15.0%
!!! CATTTTTTGGTTTTTATTTA+GGG - chr2.2:54472153-54472172 MS.gene51421:intron 15.0%
!!! TCATTTTTTGGTTTTTATTT+AGG - chr2.2:54472152-54472171 MS.gene51421:intron 15.0%
!! AACTATATTTCTTACAAAAG+TGG + chr2.2:54472751-54472770 None:intergenic 20.0%
!! TTTGTATTTATCTGATTCAT+GGG - chr2.2:54473188-54473207 MS.gene51421:intron 20.0%
!!! AAATCGAATTTTGAATCAAA+AGG + chr2.2:54471932-54471951 None:intergenic 20.0%
!!! AAGTTTAAAGCTTTTGATTT+GGG - chr2.2:54472054-54472073 MS.gene51421:intron 20.0%
!!! AATCGAATTTTGAATCAAAA+GGG + chr2.2:54471931-54471950 None:intergenic 20.0%
!!! GCTATTTTTATAAACTATCT+TGG + chr2.2:54473580-54473599 None:intergenic 20.0%
!!! TGATTTTCTCAAATTATTAG+TGG - chr2.2:54472401-54472420 MS.gene51421:intron 20.0%
! AATGTTCCATTTCTTATTCT+AGG - chr2.2:54474045-54474064 MS.gene51421:intron 25.0%
! ATAAAAATGTTACAAAGACC+AGG + chr2.2:54472503-54472522 None:intergenic 25.0%
! CTTTGTATTTATCTGATTCA+TGG - chr2.2:54473187-54473206 MS.gene51421:intron 25.0%
! GAGAAGATGAATTATTTGAA+TGG + chr2.2:54472896-54472915 None:intergenic 25.0%
! TTCAGTGTAATTATAAGTGT+CGG + chr2.2:54472376-54472395 None:intergenic 25.0%
! TTCTCTCAAATTCTTGTTAA+AGG + chr2.2:54473305-54473324 None:intergenic 25.0%
! TTGGAAAGATTATTATGCTA+AGG - chr2.2:54472101-54472120 MS.gene51421:intron 25.0%
! TTGTTTCCTAGAATAAGAAA+TGG + chr2.2:54474054-54474073 None:intergenic 25.0%
! TTTCTTACAAAAGTGGTATT+TGG + chr2.2:54472744-54472763 None:intergenic 25.0%
!! AAATACAAAGAGCTTTATCT+GGG + chr2.2:54473177-54473196 None:intergenic 25.0%
!! ACACTTTCATTTTCATGAAT+TGG - chr2.2:54472627-54472646 MS.gene51421:intron 25.0%
!! CTCTAGAAGTTATAGTATAT+TGG + chr2.2:54473881-54473900 None:intergenic 25.0%
!! TAAATACAAAGAGCTTTATC+TGG + chr2.2:54473178-54473197 None:intergenic 25.0%
!! TTTTAATGACTCATTCTTCT+TGG - chr2.2:54473096-54473115 MS.gene51421:intron 25.0%
!!! CAAGTTTAAAGCTTTTGATT+TGG - chr2.2:54472053-54472072 MS.gene51421:intron 25.0%
!!! GTTTTTATTTAGGGATTAGT+TGG - chr2.2:54472162-54472181 MS.gene51421:intron 25.0%
AACTAATTAACTAGCTAGCA+TGG + chr2.2:54472796-54472815 None:intergenic 30.0%
ACACGTTCAATATATAGTGT+GGG + chr2.2:54472834-54472853 None:intergenic 30.0%
ACTAATTAACTAGCTAGCAT+GGG + chr2.2:54472795-54472814 None:intergenic 30.0%
AGATCTAAACCCAAAAGAAT+GGG + chr2.2:54471736-54471755 None:intergenic 30.0%
ATCTTTGAGCATATGAAGAA+GGG + chr2.2:54471881-54471900 None:intergenic 30.0%
ATTAAGCCGTTTATCTTAAC+AGG - chr2.2:54473689-54473708 MS.gene51421:intron 30.0%
ATTTGTGAAAGTCTGTTGAA+AGG - chr2.2:54472205-54472224 MS.gene51421:intron 30.0%
CATACCCTTGAATTGATATA+TGG - chr2.2:54473435-54473454 MS.gene51421:intron 30.0%
TCAAAAGGGTCAAAGAAAAA+AGG + chr2.2:54471917-54471936 None:intergenic 30.0%
TCAAATGTACTAATACGTGT+CGG + chr2.2:54472866-54472885 None:intergenic 30.0%
TCATCTTCTCACATTATTAC+CGG - chr2.2:54472906-54472925 MS.gene51421:intron 30.0%
TGTCTAAAGGTGAAAGAATA+TGG + chr2.2:54473716-54473735 None:intergenic 30.0%
TTAAGCCGTTTATCTTAACA+GGG - chr2.2:54473690-54473709 MS.gene51421:intron 30.0%
TTATGTTAGCTCAAACATGT+CGG + chr2.2:54473472-54473491 None:intergenic 30.0%
! AATTTGTTTCAGCTATTGCA+TGG - chr2.2:54472235-54472254 MS.gene51421:intron 30.0%
! TGGAAATCTCTTTTGTCTAA+AGG + chr2.2:54473729-54473748 None:intergenic 30.0%
!! ACTATAACTTCTAGAGTTGT+TGG - chr2.2:54473885-54473904 MS.gene51421:CDS 30.0%
!!! TTTAAAGCTTTTGATTTGGG+TGG - chr2.2:54472057-54472076 MS.gene51421:intron 30.0%
AAACTATCTTGGAGAGCTTA+TGG + chr2.2:54473569-54473588 None:intergenic 35.0%
AAAGGAAAACAGTACATGTG+TGG + chr2.2:54473287-54473306 None:intergenic 35.0%
AAATCAGCACCTATGAAACA+CGG + chr2.2:54472449-54472468 None:intergenic 35.0%
ACAGGCCATATATCAATTCA+AGG + chr2.2:54473443-54473462 None:intergenic 35.0%
AGTCAAACATAGAGCTGAAT+TGG + chr2.2:54473819-54473838 None:intergenic 35.0%
ATCAAATTGCTCGTAGAGTT+TGG - chr2.2:54472082-54472101 MS.gene51421:intron 35.0%
CACACGTTCAATATATAGTG+TGG + chr2.2:54472835-54472854 None:intergenic 35.0%
CAGGCCATATATCAATTCAA+GGG + chr2.2:54473442-54473461 None:intergenic 35.0%
CATCTTTGAGCATATGAAGA+AGG + chr2.2:54471882-54471901 None:intergenic 35.0%
CTATATATTGAACGTGTGTC+CGG - chr2.2:54472837-54472856 MS.gene51421:intron 35.0%
GAGAGAAAGAACATACAGAA+GGG - chr2.2:54473318-54473337 MS.gene51421:intron 35.0%
GAGATCTAAACCCAAAAGAA+TGG + chr2.2:54471737-54471756 None:intergenic 35.0%
GTAACTACATGTGTAAGTGT+CGG + chr2.2:54472598-54472617 None:intergenic 35.0%
GTATTTATCTGATTCATGGG+AGG - chr2.2:54473191-54473210 MS.gene51421:intron 35.0%
TATGGCCCTGTTAAGATAAA+CGG + chr2.2:54473698-54473717 None:intergenic 35.0%
TCTTCATATGCTCAAAGATG+AGG - chr2.2:54471882-54471901 MS.gene51421:CDS 35.0%
TGAAAGTCTGTTGAAAGGTT+TGG - chr2.2:54472210-54472229 MS.gene51421:intron 35.0%
TGAGAGAAAGAACATACAGA+AGG - chr2.2:54473317-54473336 MS.gene51421:intron 35.0%
TGATATATTGTGAAAGCCAC+TGG + chr2.2:54474242-54474261 None:intergenic 35.0%
! ACAAAACCAACCCATTCTTT+TGG - chr2.2:54471723-54471742 MS.gene51421:CDS 35.0%
! AGCTTTATCTGGGATTATGA+AGG + chr2.2:54473167-54473186 None:intergenic 35.0%
! CAAAACCAACCCATTCTTTT+GGG - chr2.2:54471724-54471743 MS.gene51421:CDS 35.0%
! TTTAGAATTCTCACCTATGC+TGG - chr2.2:54473915-54473934 MS.gene51421:CDS 35.0%
!! ACACCTCCTGAAAGTTTTAA+AGG + chr2.2:54473250-54473269 None:intergenic 35.0%
!! ACTTCTAGAGTTGTTGGATA+TGG - chr2.2:54473891-54473910 MS.gene51421:CDS 35.0%
!! AGGTGTTACTTCTTGTTGTA+TGG - chr2.2:54472976-54472995 MS.gene51421:intron 35.0%
!! GGTGTTACTTCTTGTTGTAT+GGG - chr2.2:54472977-54472996 MS.gene51421:intron 35.0%
!! GTAATTATAAGTGTCGGTGT+CGG + chr2.2:54472370-54472389 None:intergenic 35.0%
!! GTGTTACTTCTTGTTGTATG+GGG - chr2.2:54472978-54472997 MS.gene51421:intron 35.0%
!! TTCATCTTCGATTGGTTCTA+TGG - chr2.2:54471778-54471797 MS.gene51421:CDS 35.0%
!!! ACCTCCTGAAAGTTTTAAAG+GGG + chr2.2:54473248-54473267 None:intergenic 35.0%
!!! CACCTCCTGAAAGTTTTAAA+GGG + chr2.2:54473249-54473268 None:intergenic 35.0%
!!! CTGATTTGTTTTCAGTTGGA+TGG - chr2.2:54472462-54472481 MS.gene51421:intron 35.0%
AAGACCAAGGAAGAGAATCT+TGG + chr2.2:54471839-54471858 None:intergenic 40.0%
ACTTCGGAGGAATTGAGTAT+TGG - chr2.2:54472024-54472043 MS.gene51421:intron 40.0%
AGAAAGAACATACAGAAGGG+TGG - chr2.2:54473321-54473340 MS.gene51421:intron 40.0%
AGCTGAATTGGTGAATGGAA+AGG + chr2.2:54473807-54473826 None:intergenic 40.0%
ATACTCAATTCCTCCGAAGT+AGG + chr2.2:54472024-54472043 None:intergenic 40.0%
CAAACATGTCGGATCAAAAC+AGG + chr2.2:54473461-54473480 None:intergenic 40.0%
CAACCATGTTCATCTTCGAT+TGG - chr2.2:54471770-54471789 MS.gene51421:CDS 40.0%
CATAGAGCTGAATTGGTGAA+TGG + chr2.2:54473812-54473831 None:intergenic 40.0%
CTAAACCCAAAAGAATGGGT+TGG + chr2.2:54471732-54471751 None:intergenic 40.0%
CTTCGGAGGAATTGAGTATT+GGG - chr2.2:54472025-54472044 MS.gene51421:intron 40.0%
GAAAACAGTACATGTGTGGA+AGG + chr2.2:54473283-54473302 None:intergenic 40.0%
GAAAGAACATACAGAAGGGT+GGG - chr2.2:54473322-54473341 MS.gene51421:intron 40.0%
GAACCAATCGAAGATGAACA+TGG + chr2.2:54471776-54471795 None:intergenic 40.0%
GAGGTTTGCAGAATCTAAGA+AGG - chr2.2:54473987-54474006 MS.gene51421:intron 40.0%
GGTCTATGTAAGTGCTTCAT+AGG - chr2.2:54472956-54472975 MS.gene51421:intron 40.0%
TGTCTGTATCCGTGTTTCAT+AGG - chr2.2:54472437-54472456 MS.gene51421:intron 40.0%
TTATTAGTGGTGTCTGTGTC+CGG - chr2.2:54472414-54472433 MS.gene51421:intron 40.0%
TTCCAGCATTATCAAGACCA+AGG + chr2.2:54471852-54471871 None:intergenic 40.0%
! CAGTGTTGTGTCTGTGATTT+TGG - chr2.2:54472661-54472680 MS.gene51421:intron 40.0%
! TTCTTTTTGCCAGAGACCAA+GGG + chr2.2:54471815-54471834 None:intergenic 40.0%
!! ACCCCTTTAAAACTTTCAGG+AGG - chr2.2:54473244-54473263 MS.gene51421:intron 40.0%
!! AGCACCCCTTTAAAACTTTC+AGG - chr2.2:54473241-54473260 MS.gene51421:intron 40.0%
!! TTCCTTGGTCTTGATAATGC+TGG - chr2.2:54471847-54471866 MS.gene51421:CDS 40.0%
!!! AGAATGGGTTGGTTTTGTGT+TGG + chr2.2:54471721-54471740 None:intergenic 40.0%
!!! GGTGCTGATTTGTTTTCAGT+TGG - chr2.2:54472458-54472477 MS.gene51421:intron 40.0%
ACCAATTTCACCACAGGCAA+AGG - chr2.2:54474129-54474148 MS.gene51421:CDS 45.0%
AGACTGTTTGTGAGAGCTTG+TGG - chr2.2:54473501-54473520 MS.gene51421:intron 45.0%
AGTTGGATGGATCTGTTGAC+AGG - chr2.2:54472475-54472494 MS.gene51421:intron 45.0%
ATTCCTCCGAAGTAGGATGT+TGG + chr2.2:54472017-54472036 None:intergenic 45.0%
ATTGTCCGCAAGATGGGATA+TGG - chr2.2:54474207-54474226 MS.gene51421:CDS 45.0%
CATGGGAGGCAAAAAGTTAC+TGG - chr2.2:54473205-54473224 MS.gene51421:intron 45.0%
CTGCACATGAACACTTCAAG+AGG + chr2.2:54474186-54474205 None:intergenic 45.0%
GAAGCCAAGATTCTCTTCCT+TGG - chr2.2:54471832-54471851 MS.gene51421:CDS 45.0%
GACGAGCTACGATATCATCT+CGG - chr2.2:54474102-54474121 MS.gene51421:CDS 45.0%
TACTAATACGTGTCGGACAC+CGG + chr2.2:54472859-54472878 None:intergenic 45.0%
TATGGCATTCTTGCATCCCT+TGG - chr2.2:54471796-54471815 MS.gene51421:CDS 45.0%
TCGATGCTTACGACAAAGAG+AGG - chr2.2:54473968-54473987 MS.gene51421:intron 45.0%
TGAAACACGGATACAGACAC+CGG + chr2.2:54472436-54472455 None:intergenic 45.0%
TGCCTGTGGTGAAATTGGTT+AGG + chr2.2:54474128-54474147 None:intergenic 45.0%
TTCCTCCGAAGTAGGATGTT+GGG + chr2.2:54472016-54472035 None:intergenic 45.0%
! CAACGCTTGAGTGTTGTGTT+TGG - chr2.2:54472935-54472954 MS.gene51421:intron 45.0%
! CTTCTTTTTGCCAGAGACCA+AGG + chr2.2:54471816-54471835 None:intergenic 45.0%
!! CGTCTTCTGAAGCTGCATAT+GGG + chr2.2:54474086-54474105 None:intergenic 45.0%
!! CTTTGTCGTAAGCATCGACT+AGG + chr2.2:54473966-54473985 None:intergenic 45.0%
!! TCGTCTTCTGAAGCTGCATA+TGG + chr2.2:54474087-54474106 None:intergenic 45.0%
!!! GGGTGCTGGATTTTCATATG+TGG - chr2.2:54472998-54473017 MS.gene51421:intron 45.0%
ACACATCCACACATCCGACA+CGG + chr2.2:54472347-54472366 None:intergenic 50.0%
ACCGACCCAACATCCTACTT+CGG - chr2.2:54472008-54472027 MS.gene51421:CDS 50.0%
ATTGACCTTGCCTTTGCCTG+TGG + chr2.2:54474142-54474161 None:intergenic 50.0%
CACTTCAAGAGGTCGAACGT+TGG + chr2.2:54474175-54474194 None:intergenic 50.0%
CTTCGCCATATCCCATCTTG+CGG + chr2.2:54474215-54474234 None:intergenic 50.0%
GCCTTTGCCTGTGGTGAAAT+TGG + chr2.2:54474133-54474152 None:intergenic 50.0%
GGCCTAACCAATTTCACCAC+AGG - chr2.2:54474123-54474142 MS.gene51421:CDS 50.0%
TCCACCTGGATAACCAGCAT+AGG + chr2.2:54473931-54473950 None:intergenic 50.0%
TCCGAAGTAGGATGTTGGGT+CGG + chr2.2:54472012-54472031 None:intergenic 50.0%
TGCAGCATTGTCCGCAAGAT+GGG - chr2.2:54474201-54474220 MS.gene51421:CDS 50.0%
TGGATCTGTTGACAGGTTCC+TGG - chr2.2:54472482-54472501 MS.gene51421:intron 50.0%
TTCTTGCATCCCTTGGTCTC+TGG - chr2.2:54471803-54471822 MS.gene51421:CDS 50.0%
TTTCACCACAGGCAAAGGCA+AGG - chr2.2:54474134-54474153 MS.gene51421:CDS 50.0%
! ACCTATGCTGGTTATCCAGG+TGG - chr2.2:54473927-54473946 MS.gene51421:CDS 50.0%
! CTCACCTATGCTGGTTATCC+AGG - chr2.2:54473924-54473943 MS.gene51421:CDS 50.0%
! CTTCTTGTTGTATGGGGTGC+TGG - chr2.2:54472984-54473003 MS.gene51421:intron 50.0%
ACATCCGACACGGCATGTGT+CGG + chr2.2:54472337-54472356 None:intergenic 55.0%
CGCAAGATGGGATATGGCGA+AGG - chr2.2:54474213-54474232 MS.gene51421:CDS 55.0%
GACCCAACATCCTACTTCGG+AGG - chr2.2:54472011-54472030 MS.gene51421:intron 55.0%
GATATGGCGAAGGCTTCCAG+TGG - chr2.2:54474223-54474242 MS.gene51421:CDS 55.0%
GGTAAACTACAGCGTCCACC+TGG + chr2.2:54473945-54473964 None:intergenic 55.0%
GTGCAGCATTGTCCGCAAGA+TGG - chr2.2:54474200-54474219 MS.gene51421:CDS 55.0%
GTGTCGGACACATGGACATG+AGG + chr2.2:54472321-54472340 None:intergenic 55.0%
!! CACTCAAGCGTTGACATCGC+CGG + chr2.2:54472928-54472947 None:intergenic 55.0%
CACATGCCGTGTCGGATGTG+TGG - chr2.2:54472338-54472357 MS.gene51421:intron 60.0%
CACGGCATGTGTCGGACACA+TGG + chr2.2:54472329-54472348 None:intergenic 60.0%
GTGTCCGACACATGCCGTGT+CGG - chr2.2:54472330-54472349 MS.gene51421:intron 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 54471708 54474266 54471708 ID=MS.gene51421
chr2.2 mRNA 54471708 54474266 54471708 ID=MS.gene51421.t1;Parent=MS.gene51421
chr2.2 exon 54474071 54474266 54474071 ID=MS.gene51421.t1.exon1;Parent=MS.gene51421.t1
chr2.2 CDS 54474071 54474266 54474071 ID=cds.MS.gene51421.t1;Parent=MS.gene51421.t1
chr2.2 exon 54473852 54473983 54473852 ID=MS.gene51421.t1.exon2;Parent=MS.gene51421.t1
chr2.2 CDS 54473852 54473983 54473852 ID=cds.MS.gene51421.t1;Parent=MS.gene51421.t1
chr2.2 exon 54471708 54472028 54471708 ID=MS.gene51421.t1.exon3;Parent=MS.gene51421.t1
chr2.2 CDS 54471708 54472028 54471708 ID=cds.MS.gene51421.t1;Parent=MS.gene51421.t1
Gene Sequence

>MS.gene51421

ATTCACAAAACCAACACAAAACCAACCCATTCTTTTGGGTTTAGATCTCTCACTCACTCTCTCAACCATGTTCATCTTCGATTGGTTCTATGGCATTCTTGCATCCCTTGGTCTCTGGCAAAAAGAAGCCAAGATTCTCTTCCTTGGTCTTGATAATGCTGGAAAAACTACCCTTCTTCATATGCTCAAAGATGAGGTTATTTAATCCTTTTTTCTTTGACCCTTTTGATTCAAAATTCGATTTTTTGAATAAATATTTGATTTTTGATGTTTTTTATTGCAGAGATTAGTTCAACATCAACCGACCCAACATCCTACTTCGGAGGAATTGAGTATTGGGAAGATCAAGTTTAAAGCTTTTGATTTGGGTGGTCATCAAATTGCTCGTAGAGTTTGGAAAGATTATTATGCTAAGGTTGAATCTCTTGATCTATTTCTTGATTATCATTTTTTGGTTTTTATTTAGGGATTAGTTGGAATTTTTTATGTGATTTTGAATTTGTGAAAGTCTGTTGAAAGGTTTGGTTAATTTGTTTCAGCTATTGCATGGTTGTTTTAAGTGATTTTTATTGTTTTGTTAGTGCTGATCTATCTATGAAACACTGACACTCCTCATGTCCATGTGTCCGACACATGCCGTGTCGGATGTGTGGATGTGTCCGACACCGACACTTATAATTACACTGAATTATGTGATTTTCTCAAATTATTAGTGGTGTCTGTGTCCGGTGTCTGTATCCGTGTTTCATAGGTGCTGATTTGTTTTCAGTTGGATGGATCTGTTGACAGGTTCCTGGTCTTTGTAACATTTTTATTGTTTCATATTTTTAGACTCTATTGATTGACAAAGTTATAAATATAATGATCTATCTAGGCGTGTCACGTGTCCGACACTTACACATGTAGTTACATTTATTTAACACTTTCATTTTCATGAATTGGTGTTATTGTGTCAGTGTTGTGTCTGTGATTTTGGTTATGATTATGATGCATCTACTACGATGTTCAAAAACTTAATTAAATGTCTTTTCATCCAAATACCACTTTTGTAAGAAATATAGTTTGATTATGTTATGACTTTCTGCCCATGCTAGCTAGTTAATTAGTTTCACTCTCTGTAGTACCCACACTATATATTGAACGTGTGTCCGGTGTCCGACACGTATTAGTACATTTGATTTGATTCCATTCAAATAATTCATCTTCTCACATTATTACCGGCGATGTCAACGCTTGAGTGTTGTGTTTGGTCTATGTAAGTGCTTCATAGGTGTTACTTCTTGTTGTATGGGGTGCTGGATTTTCATATGTGGCGATCTTTTGCTGCAGTTGCTGTAAATATTTGAATTTTTCATTCTAAAATTTGTTAGATTCTGACTTTGAGCAACTTTTAATGACTCATTCTTCTTGGTAATAATTTACACAACACAAGTGCTCACTAATCACTAAGTTTATGCCTTCATAATCCCAGATAAAGCTCTTTGTATTTATCTGATTCATGGGAGGCAAAAAGTTACTGGAAGATTCGCTGATAGCACCCCTTTAAAACTTTCAGGAGGTGTTTACTAGTTGCCTTCCACACATGTACTGTTTTCCTTTAACAAGAATTTGAGAGAAAGAACATACAGAAGGGTGGGAGAACAAATAACAGATACATAGATTATATCTGTATAAAAGGATTCAAAACTTTCTAAAAAATTTGATCATCTCATTTAAGCATTAGAAGACATACCCTTGAATTGATATATGGCCTGTTTTGATCCGACATGTTTGAGCTAACATAAGCACTTGTGAGACTGTTTGTGAGAGCTTGTGGAGACAACTTATAACATGTGCTGAAGTTTCTTGCAGCTTATTTCCATAAGCTCTCCAAGATAGTTTATAAAAATAGCTTATAATTTATATAGAAAGAGTTTGACTTTATTTTATTTTTTGTTATAGAAATAAACTTATACGTAAGCATTTAAAAGATAAGCGCTTAATTAAGCCGTTTATCTTAACAGGGCCATATTCTTTCACCTTTAGACAAAAGAGATTTCCAATAACAAAAATTTGAAGTTTTTTTGGTAAATATTGCTTAGTATCTATATACATCACCTTTCCATTCACCAATTCAGCTCTATGTTTGACTTGAGCAAATTTCTTGTGTCTTTTTTGATATGACTATATACCAATATACTATAACTTCTAGAGTTGTTGGATATGGCTTTAGAATTCTCACCTATGCTGGTTATCCAGGTGGACGCTGTAGTTTACCTAGTCGATGCTTACGACAAAGAGAGGTTTGCAGAATCTAAGAAGGAGCTTGACGCATTGCTCTCAGACGAGTCTTTAGCAAATGTTCCATTTCTTATTCTAGGAAACAAGATTGACATCCCATATGCAGCTTCAGAAGACGAGCTACGATATCATCTCGGCCTAACCAATTTCACCACAGGCAAAGGCAAGGTCAATCTTACAGACTCCAACGTTCGACCTCTTGAAGTGTTCATGTGCAGCATTGTCCGCAAGATGGGATATGGCGAAGGCTTCCAGTGGCTTTCACAATATATCAAGTAG
Protein sequence

>MS.gene51421.t1

IHKTNTKPTHSFGFRSLTHSLNHVHLRLVLWHSCIPWSLAKRSQDSLPWSUUCWKNYPSSYAQRUEISSTSTDPTSYFGGIEYWEDQVUSFUFGWSSNCSUSLERLLCUGGRCSLPSRCLRQREVCRIUEGAURIALRRVFSKCSISYSRKQDUHPICSFRRRATISSRPNQFHHRQRQGQSYRLQRSTSUSVHVQHCPQDGIWRRLPVAFTIYQV