Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51444.t1 | XP_013468646.1 | 82.3 | 299 | 52 | 1 | 1 | 299 | 1 | 298 | 3.50E-82 | 315.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51444.t1 | A0A072VML7 | 82.3 | 299 | 52 | 1 | 1 | 299 | 1 | 298 | 2.6e-82 | 315.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050901 | MS.gene51444 | 0.824306 | 8.67E-54 | -1.69E-46 |
| MS.gene055466 | MS.gene51444 | 0.803003 | 4.29E-49 | -1.69E-46 |
| MS.gene055890 | MS.gene51444 | 0.810481 | 1.13E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51444.t1 | MTR_1g073670 | 93.645 | 299 | 18 | 1 | 1 | 299 | 1 | 298 | 1.76e-170 | 474 |
| MS.gene51444.t1 | MTR_1g069610 | 26.667 | 345 | 182 | 14 | 1 | 298 | 1 | 321 | 3.41e-14 | 72.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51444.t1 | AT2G33400 | 29.967 | 307 | 138 | 10 | 1 | 288 | 1 | 249 | 2.21e-25 | 102 |
| MS.gene51444.t1 | AT5G44040 | 32.759 | 290 | 141 | 11 | 36 | 298 | 77 | 339 | 2.86e-18 | 84.7 |
| MS.gene51444.t1 | AT1G04030 | 28.086 | 324 | 173 | 14 | 1 | 285 | 1 | 303 | 1.03e-12 | 68.6 |
| MS.gene51444.t1 | AT1G04030 | 28.086 | 324 | 173 | 14 | 1 | 285 | 1 | 303 | 1.03e-12 | 68.6 |
Find 45 sgRNAs with CRISPR-Local
Find 58 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAAAGTTTGGAGAATTT+TGG | 0.099083 | 1.3:-53074209 | MS.gene51444:CDS |
| CCCTAGTTCTTATCAGGTTT+TGG | 0.222472 | 1.3:-53074438 | MS.gene51444:CDS |
| ACAAACCTGATTTGAGAATA+TGG | 0.294465 | 1.3:+53074641 | None:intergenic |
| GGCAAACCCTAGTTCTTATC+AGG | 0.299458 | 1.3:-53074444 | MS.gene51444:CDS |
| TTGTACCATATTCTCAAATC+AGG | 0.328302 | 1.3:-53074646 | MS.gene51444:intron |
| TGATGAGGAAGATGAAAATA+AGG | 0.334449 | 1.3:-53074408 | MS.gene51444:CDS |
| TGATTGGGATGATGAAAGTT+TGG | 0.346270 | 1.3:-53074219 | MS.gene51444:CDS |
| GGAGGATGAAATAGTGTATG+AGG | 0.408167 | 1.3:-53074288 | MS.gene51444:CDS |
| GAAGTTCTCGCCTTGCGTCT+TGG | 0.432670 | 1.3:-53073925 | MS.gene51444:CDS |
| TCAGTCGAAGCCTTTGTTGC+AGG | 0.437491 | 1.3:-53073889 | MS.gene51444:CDS |
| AATTTGATGAAGGGTATGAT+TGG | 0.441432 | 1.3:-53074235 | MS.gene51444:CDS |
| TGCTAGTCTTTCAAACTGGT+TGG | 0.449139 | 1.3:-53073853 | MS.gene51444:CDS |
| AAGCATGCTGCCTGTAGTCT+TGG | 0.451334 | 1.3:-53073958 | MS.gene51444:CDS |
| TTTGAATGTAGAGATGTTTG+AGG | 0.463362 | 1.3:-53074465 | MS.gene51444:CDS |
| CAACAAAGGCTTCGACTGAA+TGG | 0.464145 | 1.3:+53073893 | None:intergenic |
| TTGATGCTAGTCTTTCAAAC+TGG | 0.480004 | 1.3:-53073857 | MS.gene51444:CDS |
| AACAGTGAACAGAAAGAAGA+TGG | 0.482964 | 1.3:-53074164 | MS.gene51444:CDS |
| GATGATGATGAATTTGATGA+AGG | 0.486925 | 1.3:-53074245 | MS.gene51444:CDS |
| TAGAGGTAGAGATCAAGAAA+AGG | 0.488908 | 1.3:-53074534 | MS.gene51444:CDS |
| CATTGAGTAAGATTCTCTAC+CGG | 0.490839 | 1.3:+53074053 | None:intergenic |
| CGGTAGAGAATCTTACTCAA+TGG | 0.500520 | 1.3:-53074052 | MS.gene51444:CDS |
| ACTGCAACTTCCTGCAACAA+AGG | 0.507022 | 1.3:+53073879 | None:intergenic |
| ACTCTCTACAAAGTCCTAGC+TGG | 0.507971 | 1.3:-53074804 | MS.gene51444:CDS |
| GAGCTTGATTCCAAGACTAC+AGG | 0.512568 | 1.3:+53073948 | None:intergenic |
| TCCAAAACCTGATAAGAACT+AGG | 0.528369 | 1.3:+53074437 | None:intergenic |
| ATCTAGCAAACAAACGAGAA+AGG | 0.529514 | 1.3:-53074006 | MS.gene51444:CDS |
| CATGAATTCCGTACTGCACC+CGG | 0.549273 | 1.3:-53074072 | MS.gene51444:CDS |
| ATTCGACGGATATCGAATCA+TGG | 0.560974 | 1.3:+53074346 | None:intergenic |
| CCAAAACCTGATAAGAACTA+GGG | 0.562445 | 1.3:+53074438 | None:intergenic |
| GATCAAGAAAAGGAGAAGAG+TGG | 0.564885 | 1.3:-53074524 | MS.gene51444:CDS |
| TAGTTATGACAATGAGGAGG+AGG | 0.567704 | 1.3:-53074306 | MS.gene51444:CDS |
| ATTTGATGAAGGGTATGATT+GGG | 0.573696 | 1.3:-53074234 | MS.gene51444:CDS |
| ATTCTCTACCGGGTGCAGTA+CGG | 0.574059 | 1.3:+53074064 | None:intergenic |
| ATGATGATGAATTTGATGAA+GGG | 0.582122 | 1.3:-53074244 | MS.gene51444:CDS |
| ATTGAGTAAGATTCTCTACC+GGG | 0.594847 | 1.3:+53074054 | None:intergenic |
| TTATGATATCTATGATTCGA+CGG | 0.606450 | 1.3:+53074332 | None:intergenic |
| CGAATTTGAGTGGACGAGAG+AGG | 0.606574 | 1.3:-53074103 | MS.gene51444:CDS |
| GAATTGAAATCGAATTTGAG+TGG | 0.620274 | 1.3:-53074113 | MS.gene51444:CDS |
| TCATTGACAACGAATTGTAG+TGG | 0.620784 | 1.3:+53073809 | None:intergenic |
| TGAAAATAAGGAGACTACTG+TGG | 0.644236 | 1.3:-53074396 | MS.gene51444:CDS |
| TAACTGTAGTTATGACAATG+AGG | 0.650246 | 1.3:-53074312 | MS.gene51444:CDS |
| GCATTCGATTCCAAGACGCA+AGG | 0.653152 | 1.3:+53073915 | None:intergenic |
| GTAGTGGAGACATTATAACT+AGG | 0.665616 | 1.3:+53073825 | None:intergenic |
| ACTGTGGAATCAGAGAGAGA+AGG | 0.685984 | 1.3:-53074380 | MS.gene51444:CDS |
| CTGTAGTTATGACAATGAGG+AGG | 0.688122 | 1.3:-53074309 | MS.gene51444:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTTTTTAATTTATTTGTTAG+AGG | - | chr1.3:53074116-53074135 | MS.gene51444:CDS | 10.0% |
| !! | TGAAGAAATATTATTGTATG+TGG | - | chr1.3:53074068-53074087 | MS.gene51444:CDS | 20.0% |
| ! | AATCAAGAACTTAAGAATGA+TGG | + | chr1.3:53073918-53073937 | None:intergenic | 25.0% |
| ! | ATCAAGAACTTAAGAATGAT+GGG | + | chr1.3:53073917-53073936 | None:intergenic | 25.0% |
| ! | TTATGATATCTATGATTCGA+CGG | + | chr1.3:53074338-53074357 | None:intergenic | 25.0% |
| !! | ATGAAAGTTTGGAGAATTTT+GGG | - | chr1.3:53074459-53074478 | MS.gene51444:CDS | 25.0% |
| !! | ATGATGATGAATTTGATGAA+GGG | - | chr1.3:53074423-53074442 | MS.gene51444:CDS | 25.0% |
| !! | CTTATCTTTTTGCAGAAATA+TGG | - | chr1.3:53073960-53073979 | MS.gene51444:CDS | 25.0% |
| !!! | GTTGTTTTGATATACTTACT+TGG | + | chr1.3:53073895-53073914 | None:intergenic | 25.0% |
| GAATTGAAATCGAATTTGAG+TGG | - | chr1.3:53074554-53074573 | MS.gene51444:intron | 30.0% | |
| TAACTGTAGTTATGACAATG+AGG | - | chr1.3:53074355-53074374 | MS.gene51444:CDS | 30.0% | |
| TGATGAGGAAGATGAAAATA+AGG | - | chr1.3:53074259-53074278 | MS.gene51444:CDS | 30.0% | |
| TTGTACCATATTCTCAAATC+AGG | - | chr1.3:53074021-53074040 | MS.gene51444:CDS | 30.0% | |
| TTTGAATGTAGAGATGTTTG+AGG | - | chr1.3:53074202-53074221 | MS.gene51444:CDS | 30.0% | |
| ! | AATTTGATGAAGGGTATGAT+TGG | - | chr1.3:53074432-53074451 | MS.gene51444:CDS | 30.0% |
| ! | ACAAACCTGATTTGAGAATA+TGG | + | chr1.3:53074029-53074048 | None:intergenic | 30.0% |
| ! | ATTTGATGAAGGGTATGATT+GGG | - | chr1.3:53074433-53074452 | MS.gene51444:CDS | 30.0% |
| ! | GATGAAAGTTTGGAGAATTT+TGG | - | chr1.3:53074458-53074477 | MS.gene51444:CDS | 30.0% |
| ! | GATGATGATGAATTTGATGA+AGG | - | chr1.3:53074422-53074441 | MS.gene51444:CDS | 30.0% |
| ! | TGAAAGTTTGGAGAATTTTG+GGG | - | chr1.3:53074460-53074479 | MS.gene51444:CDS | 30.0% |
| AACAGTGAACAGAAAGAAGA+TGG | - | chr1.3:53074503-53074522 | MS.gene51444:CDS | 35.0% | |
| ATCTAGCAAACAAACGAGAA+AGG | - | chr1.3:53074661-53074680 | MS.gene51444:CDS | 35.0% | |
| ATTGAGTAAGATTCTCTACC+GGG | + | chr1.3:53074616-53074635 | None:intergenic | 35.0% | |
| ATTGTATGTGGATCTGTGAA+TGG | - | chr1.3:53074080-53074099 | MS.gene51444:CDS | 35.0% | |
| CATTGAGTAAGATTCTCTAC+CGG | + | chr1.3:53074617-53074636 | None:intergenic | 35.0% | |
| CCAAAACCTGATAAGAACTA+GGG | + | chr1.3:53074232-53074251 | None:intergenic | 35.0% | |
| TAGAGGTAGAGATCAAGAAA+AGG | - | chr1.3:53074133-53074152 | MS.gene51444:CDS | 35.0% | |
| TCATTGACAACGAATTGTAG+TGG | + | chr1.3:53074861-53074880 | None:intergenic | 35.0% | |
| TCCAAAACCTGATAAGAACT+AGG | + | chr1.3:53074233-53074252 | None:intergenic | 35.0% | |
| TGAAAATAAGGAGACTACTG+TGG | - | chr1.3:53074271-53074290 | MS.gene51444:CDS | 35.0% | |
| TGATTGGGATGATGAAAGTT+TGG | - | chr1.3:53074448-53074467 | MS.gene51444:CDS | 35.0% | |
| ! | GTAGTGGAGACATTATAACT+AGG | + | chr1.3:53074845-53074864 | None:intergenic | 35.0% |
| !! | GGTTGTTTTCTTCAGTGTTT+TGG | - | chr1.3:53073812-53073831 | MS.gene51444:CDS | 35.0% |
| !! | TTGATGCTAGTCTTTCAAAC+TGG | - | chr1.3:53074810-53074829 | MS.gene51444:CDS | 35.0% |
| !!! | ATGCTTGTTTTTTGCTCTGA+TGG | + | chr1.3:53074695-53074714 | None:intergenic | 35.0% |
| ATTCGACGGATATCGAATCA+TGG | + | chr1.3:53074324-53074343 | None:intergenic | 40.0% | |
| CGGTAGAGAATCTTACTCAA+TGG | - | chr1.3:53074615-53074634 | MS.gene51444:intron | 40.0% | |
| CTGTAGTTATGACAATGAGG+AGG | - | chr1.3:53074358-53074377 | MS.gene51444:CDS | 40.0% | |
| GATCAAGAAAAGGAGAAGAG+TGG | - | chr1.3:53074143-53074162 | MS.gene51444:CDS | 40.0% | |
| GGAGGATGAAATAGTGTATG+AGG | - | chr1.3:53074379-53074398 | MS.gene51444:CDS | 40.0% | |
| TAGTTATGACAATGAGGAGG+AGG | - | chr1.3:53074361-53074380 | MS.gene51444:CDS | 40.0% | |
| !! | CCCTAGTTCTTATCAGGTTT+TGG | - | chr1.3:53074229-53074248 | MS.gene51444:CDS | 40.0% |
| !! | TGCTAGTCTTTCAAACTGGT+TGG | - | chr1.3:53074814-53074833 | MS.gene51444:CDS | 40.0% |
| !! | TTACTTGGTTTTCTCCAGCT+AGG | + | chr1.3:53073880-53073899 | None:intergenic | 40.0% |
| !!! | GGTTTTGGATGATGATGATG+AGG | - | chr1.3:53074244-53074263 | MS.gene51444:CDS | 40.0% |
| ACTCTCTACAAAGTCCTAGC+TGG | - | chr1.3:53073863-53073882 | MS.gene51444:CDS | 45.0% | |
| ACTGCAACTTCCTGCAACAA+AGG | + | chr1.3:53074791-53074810 | None:intergenic | 45.0% | |
| ACTGTGGAATCAGAGAGAGA+AGG | - | chr1.3:53074287-53074306 | MS.gene51444:CDS | 45.0% | |
| CAACAAAGGCTTCGACTGAA+TGG | + | chr1.3:53074777-53074796 | None:intergenic | 45.0% | |
| GAGCTTGATTCCAAGACTAC+AGG | + | chr1.3:53074722-53074741 | None:intergenic | 45.0% | |
| ! | GGCAAACCCTAGTTCTTATC+AGG | - | chr1.3:53074223-53074242 | MS.gene51444:CDS | 45.0% |
| AAGCATGCTGCCTGTAGTCT+TGG | - | chr1.3:53074709-53074728 | MS.gene51444:intron | 50.0% | |
| ATTCTCTACCGGGTGCAGTA+CGG | + | chr1.3:53074606-53074625 | None:intergenic | 50.0% | |
| CATGAATTCCGTACTGCACC+CGG | - | chr1.3:53074595-53074614 | MS.gene51444:intron | 50.0% | |
| CGAATTTGAGTGGACGAGAG+AGG | - | chr1.3:53074564-53074583 | MS.gene51444:intron | 50.0% | |
| GCATTCGATTCCAAGACGCA+AGG | + | chr1.3:53074755-53074774 | None:intergenic | 50.0% | |
| TCAGTCGAAGCCTTTGTTGC+AGG | - | chr1.3:53074778-53074797 | MS.gene51444:intron | 50.0% | |
| GAAGTTCTCGCCTTGCGTCT+TGG | - | chr1.3:53074742-53074761 | MS.gene51444:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 53073809 | 53074880 | 53073809 | ID=MS.gene51444 |
| chr1.3 | mRNA | 53073809 | 53074880 | 53073809 | ID=MS.gene51444.t1;Parent=MS.gene51444 |
| chr1.3 | exon | 53074794 | 53074880 | 53074794 | ID=MS.gene51444.t1.exon1;Parent=MS.gene51444.t1 |
| chr1.3 | CDS | 53074794 | 53074880 | 53074794 | ID=cds.MS.gene51444.t1;Parent=MS.gene51444.t1 |
| chr1.3 | exon | 53074647 | 53074714 | 53074647 | ID=MS.gene51444.t1.exon2;Parent=MS.gene51444.t1 |
| chr1.3 | CDS | 53074647 | 53074714 | 53074647 | ID=cds.MS.gene51444.t1;Parent=MS.gene51444.t1 |
| chr1.3 | exon | 53073809 | 53074553 | 53073809 | ID=MS.gene51444.t1.exon3;Parent=MS.gene51444.t1 |
| chr1.3 | CDS | 53073809 | 53074553 | 53073809 | ID=cds.MS.gene51444.t1;Parent=MS.gene51444.t1 |
| Gene Sequence |
| Protein sequence |
