Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51467.t1 | XP_013468200.1 | 100 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 1.40E-43 | 185.3 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51467.t1 | O50003 | 94.8 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 2.4e-44 | 179.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51467.t1 | B7FH91 | 100.0 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 9.9e-44 | 185.3 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050134 | MS.gene51467 | 0.819069 | 1.41E-52 | -1.69E-46 |
| MS.gene057738 | MS.gene51467 | 0.848991 | 4.31E-60 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51467.t1 | MTR_4g059400 | 100.000 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 2.14e-63 | 189 |
| MS.gene51467.t1 | MTR_1g064500 | 100.000 | 97 | 0 | 0 | 1 | 97 | 1 | 97 | 2.24e-63 | 189 |
| MS.gene51467.t1 | MTR_2g094300 | 87.629 | 97 | 12 | 0 | 1 | 97 | 1 | 97 | 8.57e-56 | 170 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51467.t1 | AT2G37190 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 3.28e-60 | 181 |
| MS.gene51467.t1 | AT5G60670 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 3.58e-60 | 181 |
| MS.gene51467.t1 | AT3G53430 | 94.845 | 97 | 5 | 0 | 1 | 97 | 1 | 97 | 3.70e-60 | 181 |
Find 41 sgRNAs with CRISPR-Local
Find 41 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTCTTCTTGCGATCCCTTTC+TGG | 0.120389 | 3.4:-2185565 | None:intergenic |
| GGACGTAAACTTCGACGATT+TGG | 0.192090 | 3.4:-2185324 | None:intergenic |
| CCAATCCTTGGCGGTTTCTT+TGG | 0.201986 | 3.4:-2185440 | None:intergenic |
| ATGTCTTCTCCGATCTTCTT+TGG | 0.296410 | 3.4:-2185415 | None:intergenic |
| GACGTAAACTTCGACGATTT+GGG | 0.369524 | 3.4:-2185323 | None:intergenic |
| ATTTGGGATGGATCGAGCTT+TGG | 0.421723 | 3.4:-2185307 | None:intergenic |
| TGCGGCGGCGCTGGTTATCA+AGG | 0.430495 | 3.4:+2185531 | MS.gene51467:CDS |
| CCAAAGAAACCGCCAAGGAT+TGG | 0.444226 | 3.4:+2185440 | MS.gene51467:CDS |
| GATCTTGGGAGCGAGGGAAC+TGG | 0.450092 | 3.4:-2185374 | None:intergenic |
| TGTTCCGTCTGCGGCGGCGC+TGG | 0.455070 | 3.4:+2185522 | MS.gene51467:CDS |
| CAGACCCTTCCAATCCTTGG+CGG | 0.455684 | 3.4:-2185449 | None:intergenic |
| GAGACCGAGAGGACCGATCT+TGG | 0.459185 | 3.4:-2185389 | None:intergenic |
| GGCTTTGAAGGAACCAGAAA+GGG | 0.471774 | 3.4:+2185552 | MS.gene51467:CDS |
| CCTCAGACCCTTCCAATCCT+TGG | 0.473920 | 3.4:-2185452 | None:intergenic |
| GAAGTTTACGTCCGCGTCAC+CGG | 0.477456 | 3.4:+2185334 | MS.gene51467:CDS |
| GCTGGTTATCAAGGCTTTGA+AGG | 0.478691 | 3.4:+2185540 | MS.gene51467:CDS |
| AGACCGAGAGGACCGATCTT+GGG | 0.482147 | 3.4:-2185388 | None:intergenic |
| CGCGTCACCGGCGGTGAAGT+CGG | 0.482477 | 3.4:+2185346 | MS.gene51467:CDS |
| AGTTCCCTCGCTCCCAAGAT+CGG | 0.497799 | 3.4:+2185376 | MS.gene51467:CDS |
| TGGGATGGATCGAGCTTTGG+CGG | 0.507491 | 3.4:-2185304 | None:intergenic |
| CGCTGTTGTTCCGTCTGCGG+CGG | 0.521792 | 3.4:+2185516 | MS.gene51467:CDS |
| CCAAGGATTGGAAGGGTCTG+AGG | 0.527691 | 3.4:+2185452 | MS.gene51467:CDS |
| GCTCCCAAGATCGGTCCTCT+CGG | 0.529023 | 3.4:+2185385 | MS.gene51467:CDS |
| GGCGGCTCCGACTTCACCGC+CGG | 0.532286 | 3.4:-2185353 | None:intergenic |
| GCTCACTGTTCAGAATCGTC+AGG | 0.539340 | 3.4:+2185486 | MS.gene51467:CDS |
| AGAAACCGCCAAGGATTGGA+AGG | 0.540852 | 3.4:+2185444 | MS.gene51467:CDS |
| TGTTCAGAATCGTCAGGCTA+AGG | 0.548234 | 3.4:+2185492 | MS.gene51467:CDS |
| AGGCTTTGAAGGAACCAGAA+AGG | 0.555297 | 3.4:+2185551 | MS.gene51467:CDS |
| AGGACCGATCTTGGGAGCGA+GGG | 0.558873 | 3.4:-2185380 | None:intergenic |
| CGACTTCACCGCCGGTGACG+CGG | 0.560163 | 3.4:-2185345 | None:intergenic |
| GAGGACCGATCTTGGGAGCG+AGG | 0.581430 | 3.4:-2185381 | None:intergenic |
| GGTCTCTCACCAAAGAAGAT+CGG | 0.586947 | 3.4:+2185406 | MS.gene51467:CDS |
| ATAACCAGCGCCGCCGCAGA+CGG | 0.589868 | 3.4:-2185526 | None:intergenic |
| GTTTACGTCCGCGTCACCGG+CGG | 0.607165 | 3.4:+2185337 | MS.gene51467:CDS |
| TAAACTTCGACGATTTGGGA+TGG | 0.609008 | 3.4:-2185319 | None:intergenic |
| CAAGGATTGGAAGGGTCTGA+GGG | 0.614585 | 3.4:+2185453 | MS.gene51467:CDS |
| CTTGGGAGCGAGGGAACTGG+CGG | 0.624967 | 3.4:-2185371 | None:intergenic |
| GGTCGCTGTTGTTCCGTCTG+CGG | 0.635590 | 3.4:+2185513 | MS.gene51467:CDS |
| TTCTTTGGTGAGAGACCGAG+AGG | 0.661014 | 3.4:-2185400 | None:intergenic |
| GAAACCGCCAAGGATTGGAA+GGG | 0.668035 | 3.4:+2185445 | MS.gene51467:CDS |
| CATCGCCAAAGAAACCGCCA+AGG | 0.687884 | 3.4:+2185435 | MS.gene51467:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ATGTCTTCTCCGATCTTCTT+TGG | - | chr3.4:2185418-2185437 | None:intergenic | 40.0% | |
| GACGTAAACTTCGACGATTT+GGG | - | chr3.4:2185326-2185345 | None:intergenic | 40.0% | |
| ! | TAAACTTCGACGATTTGGGA+TGG | - | chr3.4:2185322-2185341 | None:intergenic | 40.0% |
| GGACGTAAACTTCGACGATT+TGG | - | chr3.4:2185327-2185346 | None:intergenic | 45.0% | |
| GGTCTCTCACCAAAGAAGAT+CGG | + | chr3.4:2185406-2185425 | MS.gene51467:CDS | 45.0% | |
| TGTTCAGAATCGTCAGGCTA+AGG | + | chr3.4:2185492-2185511 | MS.gene51467:CDS | 45.0% | |
| ! | ATTTGGGATGGATCGAGCTT+TGG | - | chr3.4:2185310-2185329 | None:intergenic | 45.0% |
| ! | GCTGGTTATCAAGGCTTTGA+AGG | + | chr3.4:2185540-2185559 | MS.gene51467:CDS | 45.0% |
| !! | AGGCTTTGAAGGAACCAGAA+AGG | + | chr3.4:2185551-2185570 | MS.gene51467:CDS | 45.0% |
| !! | GGCTTTGAAGGAACCAGAAA+GGG | + | chr3.4:2185552-2185571 | MS.gene51467:CDS | 45.0% |
| AGAAACCGCCAAGGATTGGA+AGG | + | chr3.4:2185444-2185463 | MS.gene51467:CDS | 50.0% | |
| CAAGGATTGGAAGGGTCTGA+GGG | + | chr3.4:2185453-2185472 | MS.gene51467:CDS | 50.0% | |
| CCAAAGAAACCGCCAAGGAT+TGG | + | chr3.4:2185440-2185459 | MS.gene51467:CDS | 50.0% | |
| CCAATCCTTGGCGGTTTCTT+TGG | - | chr3.4:2185443-2185462 | None:intergenic | 50.0% | |
| GAAACCGCCAAGGATTGGAA+GGG | + | chr3.4:2185445-2185464 | MS.gene51467:CDS | 50.0% | |
| GCTCACTGTTCAGAATCGTC+AGG | + | chr3.4:2185486-2185505 | MS.gene51467:CDS | 50.0% | |
| GTCTTCTTGCGATCCCTTTC+TGG | - | chr3.4:2185568-2185587 | None:intergenic | 50.0% | |
| TTCTTTGGTGAGAGACCGAG+AGG | - | chr3.4:2185403-2185422 | None:intergenic | 50.0% | |
| AGACCGAGAGGACCGATCTT+GGG | - | chr3.4:2185391-2185410 | None:intergenic | 55.0% | |
| AGTTCCCTCGCTCCCAAGAT+CGG | + | chr3.4:2185376-2185395 | MS.gene51467:CDS | 55.0% | |
| CAGACCCTTCCAATCCTTGG+CGG | - | chr3.4:2185452-2185471 | None:intergenic | 55.0% | |
| CATCGCCAAAGAAACCGCCA+AGG | + | chr3.4:2185435-2185454 | MS.gene51467:CDS | 55.0% | |
| CCAAGGATTGGAAGGGTCTG+AGG | + | chr3.4:2185452-2185471 | MS.gene51467:CDS | 55.0% | |
| CCTCAGACCCTTCCAATCCT+TGG | - | chr3.4:2185455-2185474 | None:intergenic | 55.0% | |
| GAAGTTTACGTCCGCGTCAC+CGG | + | chr3.4:2185334-2185353 | MS.gene51467:CDS | 55.0% | |
| ! | TGGGATGGATCGAGCTTTGG+CGG | - | chr3.4:2185307-2185326 | None:intergenic | 55.0% |
| AGGACCGATCTTGGGAGCGA+GGG | - | chr3.4:2185383-2185402 | None:intergenic | 60.0% | |
| GAGACCGAGAGGACCGATCT+TGG | - | chr3.4:2185392-2185411 | None:intergenic | 60.0% | |
| GATCTTGGGAGCGAGGGAAC+TGG | - | chr3.4:2185377-2185396 | None:intergenic | 60.0% | |
| GCTCCCAAGATCGGTCCTCT+CGG | + | chr3.4:2185385-2185404 | MS.gene51467:CDS | 60.0% | |
| GGTCGCTGTTGTTCCGTCTG+CGG | + | chr3.4:2185513-2185532 | MS.gene51467:CDS | 60.0% | |
| ATAACCAGCGCCGCCGCAGA+CGG | - | chr3.4:2185529-2185548 | None:intergenic | 65.0% | |
| CGCTGTTGTTCCGTCTGCGG+CGG | + | chr3.4:2185516-2185535 | MS.gene51467:CDS | 65.0% | |
| CTTGGGAGCGAGGGAACTGG+CGG | - | chr3.4:2185374-2185393 | None:intergenic | 65.0% | |
| GAGGACCGATCTTGGGAGCG+AGG | - | chr3.4:2185384-2185403 | None:intergenic | 65.0% | |
| GTTTACGTCCGCGTCACCGG+CGG | + | chr3.4:2185337-2185356 | MS.gene51467:CDS | 65.0% | |
| !! | TGCGGCGGCGCTGGTTATCA+AGG | + | chr3.4:2185531-2185550 | MS.gene51467:CDS | 65.0% |
| CGACTTCACCGCCGGTGACG+CGG | - | chr3.4:2185348-2185367 | None:intergenic | 70.0% | |
| CGCGTCACCGGCGGTGAAGT+CGG | + | chr3.4:2185346-2185365 | MS.gene51467:CDS | 70.0% | |
| GGCGGCTCCGACTTCACCGC+CGG | - | chr3.4:2185356-2185375 | None:intergenic | 75.0% | |
| TGTTCCGTCTGCGGCGGCGC+TGG | + | chr3.4:2185522-2185541 | MS.gene51467:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.4 | gene | 2185301 | 2185594 | 2185301 | ID=MS.gene51467 |
| chr3.4 | mRNA | 2185301 | 2185594 | 2185301 | ID=MS.gene51467.t1;Parent=MS.gene51467 |
| chr3.4 | exon | 2185301 | 2185594 | 2185301 | ID=MS.gene51467.t1.exon1;Parent=MS.gene51467.t1 |
| chr3.4 | CDS | 2185301 | 2185594 | 2185301 | ID=cds.MS.gene51467.t1;Parent=MS.gene51467.t1 |
| Gene Sequence |
| Protein sequence |
