AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene050134


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050134.t1 MTR_4g059400 99.398 166 1 0 1 166 1 166 1.89e-116 327
MS.gene050134.t1 MTR_1g064500 99.390 164 1 0 1 164 1 164 2.24e-114 321
MS.gene050134.t1 MTR_2g094300 83.537 164 27 0 1 164 1 164 1.50e-97 279
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene050134.t1 AT5G60670 90.361 166 16 0 1 166 1 166 9.53e-108 305
MS.gene050134.t1 AT2G37190 89.759 166 17 0 1 166 1 166 1.20e-107 304
MS.gene050134.t1 AT3G53430 89.157 166 18 0 1 166 1 166 2.70e-107 303

Find 64 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTCTTCTTGCGGTCCCTTTC+TGG 0.085933 4.2:-44210622 None:intergenic
CGGAACGGTGAAGGAGATTT+TGG 0.108347 4.2:+44210738 MS.gene050134:CDS
GGACGTAAACTTCGACGATT+TGG 0.192090 4.2:-44210381 None:intergenic
CCAATCCTTGGCGGTTTCTT+TGG 0.201986 4.2:-44210497 None:intergenic
CCAAGGATTGGAAGGGTCTT+AGG 0.217246 4.2:+44210509 MS.gene050134:CDS
ATGTCTTCTCCGATCTTCTT+TGG 0.296410 4.2:-44210472 None:intergenic
ACTAAGAACATCAAGCATAA+TGG 0.336453 4.2:+44210643 MS.gene050134:CDS
TCTGTTGGGTGTACTGTTGA+TGG 0.361476 4.2:+44210772 MS.gene050134:CDS
CTAAGAACATCAAGCATAAT+GGG 0.363010 4.2:+44210644 MS.gene050134:CDS
GACGTAAACTTCGACGATTT+GGG 0.369524 4.2:-44210380 None:intergenic
CAAAATCTCCTTCACCGTTC+CGG 0.392918 4.2:-44210737 None:intergenic
ATCTCCTGCTGCAAATCCTT+TGG 0.401059 4.2:-44210802 None:intergenic
ATTTGGGATGGATCGAGCTT+TGG 0.421723 4.2:-44210364 None:intergenic
TTGGGGACTTGTGTTTCTGT+TGG 0.422484 4.2:+44210757 MS.gene050134:CDS
TGCGGCGGCGCTGGTTATCA+AGG 0.430495 4.2:+44210588 MS.gene050134:CDS
CCAAAGAAACCGCCAAGGAT+TGG 0.444226 4.2:+44210497 MS.gene050134:CDS
TGGGGACTTGTGTTTCTGTT+GGG 0.450633 4.2:+44210758 MS.gene050134:CDS
TGTTCCGTCTGCGGCGGCGC+TGG 0.455070 4.2:+44210579 MS.gene050134:CDS
GAGACCGAGAGGACCGATCT+TGG 0.459185 4.2:-44210446 None:intergenic
GGCTTTGAAGGAACCAGAAA+GGG 0.473237 4.2:+44210609 MS.gene050134:CDS
GATCTTGGGAGCGAGGGAAC+TGG 0.475143 4.2:-44210431 None:intergenic
GAAGTTTACGTCCGCGTCAC+CGG 0.477456 4.2:+44210391 MS.gene050134:CDS
GCTGGTTATCAAGGCTTTGA+AGG 0.478691 4.2:+44210597 MS.gene050134:CDS
AGACCGAGAGGACCGATCTT+GGG 0.482147 4.2:-44210445 None:intergenic
CGCGTCACCGGCGGTGAAGT+CGG 0.482477 4.2:+44210403 MS.gene050134:CDS
AGTTCCCTCGCTCCCAAGAT+CGG 0.485343 4.2:+44210433 MS.gene050134:CDS
TCGATGGCGAAGGAGCTTGC+CGG 0.485429 4.2:+44210718 MS.gene050134:CDS
CAAGGATTGGAAGGGTCTTA+GGG 0.485831 4.2:+44210510 MS.gene050134:CDS
AAGACCCTTCCAATCCTTGG+CGG 0.491678 4.2:-44210506 None:intergenic
GATTATGAAGCCGAGGTCGA+TGG 0.494531 4.2:+44210702 MS.gene050134:CDS
CTGTTGGGTGTACTGTTGAT+GGG 0.503488 4.2:+44210773 MS.gene050134:CDS
CCTAAGACCCTTCCAATCCT+TGG 0.507224 4.2:-44210509 None:intergenic
TGGGATGGATCGAGCTTTGG+CGG 0.507491 4.2:-44210361 None:intergenic
GGCGAAGGAGCTTGCCGGAA+CGG 0.508951 4.2:+44210723 MS.gene050134:CDS
CGCTGTTGTTCCGTCTGCGG+CGG 0.521792 4.2:+44210573 MS.gene050134:CDS
GCTCCCAAGATCGGTCCTCT+CGG 0.529023 4.2:+44210442 MS.gene050134:CDS
GGAGCTTGCCGGAACGGTGA+AGG 0.535229 4.2:+44210729 MS.gene050134:CDS
AGCTCCTTCGCCATCGACCT+CGG 0.537913 4.2:-44210712 None:intergenic
AGAAACCGCCAAGGATTGGA+AGG 0.540852 4.2:+44210501 MS.gene050134:CDS
CGTTCAGAATCGTCAGGCTA+AGG 0.541896 4.2:+44210549 MS.gene050134:CDS
GTTGAGATTCCTCAGGATTG+AGG 0.542687 4.2:+44210838 MS.gene050134:CDS
GGCAGCTCCGACTTCACCGC+CGG 0.547290 4.2:-44210410 None:intergenic
GCTCACCGTTCAGAATCGTC+AGG 0.553915 4.2:+44210543 MS.gene050134:CDS
AGATCCAAAGGATTTGCAGC+AGG 0.556469 4.2:+44210798 MS.gene050134:CDS
AGGCTTTGAAGGAACCAGAA+AGG 0.556759 4.2:+44210608 MS.gene050134:CDS
TGATGTTCTTAGTCTTCTTG+CGG 0.558436 4.2:-44210633 None:intergenic
AGGACCGATCTTGGGAGCGA+GGG 0.558873 4.2:-44210437 None:intergenic
CGACTTCACCGCCGGTGACG+CGG 0.560163 4.2:-44210402 None:intergenic
TGGTGATGTTGAGATTCCTC+AGG 0.564181 4.2:+44210831 MS.gene050134:CDS
GAGGACCGATCTTGGGAGCG+AGG 0.581430 4.2:-44210438 None:intergenic
GGTCTCTCACCAAAGAAGAT+CGG 0.586947 4.2:+44210463 MS.gene050134:CDS
ATAACCAGCGCCGCCGCAGA+CGG 0.589868 4.2:-44210583 None:intergenic
TTGCAGCAGGAGATTAGTGA+TGG 0.592966 4.2:+44210811 MS.gene050134:CDS
GAAGCCGAGGTCGATGGCGA+AGG 0.599042 4.2:+44210708 MS.gene050134:CDS
GTTTACGTCCGCGTCACCGG+CGG 0.607165 4.2:+44210394 MS.gene050134:CDS
TAAACTTCGACGATTTGGGA+TGG 0.609008 4.2:-44210376 None:intergenic
TGTTGATGGGAAAGATCCAA+AGG 0.621231 4.2:+44210786 MS.gene050134:CDS
GGTCGCTGTTGTTCCGTCTG+CGG 0.635590 4.2:+44210570 MS.gene050134:CDS
TTGCGAAGATTATGAAGCCG+AGG 0.654828 4.2:+44210695 MS.gene050134:CDS
TTCTTTGGTGAGAGACCGAG+AGG 0.661014 4.2:-44210457 None:intergenic
CTTAGCCTGACGATTCTGAA+CGG 0.665775 4.2:-44210548 None:intergenic
GAAACCGCCAAGGATTGGAA+GGG 0.668035 4.2:+44210502 MS.gene050134:CDS
CATCGCCAAAGAAACCGCCA+AGG 0.687884 4.2:+44210492 MS.gene050134:CDS
CAACAACCACCTCAATCCTG+AGG 0.689306 4.2:-44210847 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
ACTAAGAACATCAAGCATAA+TGG + chr4.2:44210643-44210662 MS.gene050134:CDS 30.0%
CTAAGAACATCAAGCATAAT+GGG + chr4.2:44210644-44210663 MS.gene050134:CDS 30.0%
! TGATGTTCTTAGTCTTCTTG+CGG - chr4.2:44210636-44210655 None:intergenic 35.0%
ATGTCTTCTCCGATCTTCTT+TGG - chr4.2:44210475-44210494 None:intergenic 40.0%
GACGTAAACTTCGACGATTT+GGG - chr4.2:44210383-44210402 None:intergenic 40.0%
! TAAACTTCGACGATTTGGGA+TGG - chr4.2:44210379-44210398 None:intergenic 40.0%
! TGTTGATGGGAAAGATCCAA+AGG + chr4.2:44210786-44210805 MS.gene050134:CDS 40.0%
AGATCCAAAGGATTTGCAGC+AGG + chr4.2:44210798-44210817 MS.gene050134:CDS 45.0%
ATCTCCTGCTGCAAATCCTT+TGG - chr4.2:44210805-44210824 None:intergenic 45.0%
CAAAATCTCCTTCACCGTTC+CGG - chr4.2:44210740-44210759 None:intergenic 45.0%
CAAGGATTGGAAGGGTCTTA+GGG + chr4.2:44210510-44210529 MS.gene050134:CDS 45.0%
CTGTTGGGTGTACTGTTGAT+GGG + chr4.2:44210773-44210792 MS.gene050134:CDS 45.0%
CTTAGCCTGACGATTCTGAA+CGG - chr4.2:44210551-44210570 None:intergenic 45.0%
GGACGTAAACTTCGACGATT+TGG - chr4.2:44210384-44210403 None:intergenic 45.0%
GGTCTCTCACCAAAGAAGAT+CGG + chr4.2:44210463-44210482 MS.gene050134:CDS 45.0%
TCTGTTGGGTGTACTGTTGA+TGG + chr4.2:44210772-44210791 MS.gene050134:CDS 45.0%
TTGCAGCAGGAGATTAGTGA+TGG + chr4.2:44210811-44210830 MS.gene050134:CDS 45.0%
TTGCGAAGATTATGAAGCCG+AGG + chr4.2:44210695-44210714 MS.gene050134:CDS 45.0%
! ATTTGGGATGGATCGAGCTT+TGG - chr4.2:44210367-44210386 None:intergenic 45.0%
! GAACGGTGAAGGAGATTTTG+GGG + chr4.2:44210740-44210759 MS.gene050134:CDS 45.0%
! GCTGGTTATCAAGGCTTTGA+AGG + chr4.2:44210597-44210616 MS.gene050134:CDS 45.0%
! GGAACGGTGAAGGAGATTTT+GGG + chr4.2:44210739-44210758 MS.gene050134:CDS 45.0%
! TGGGGACTTGTGTTTCTGTT+GGG + chr4.2:44210758-44210777 MS.gene050134:CDS 45.0%
! TGGTGATGTTGAGATTCCTC+AGG + chr4.2:44210831-44210850 MS.gene050134:CDS 45.0%
! TTGGGGACTTGTGTTTCTGT+TGG + chr4.2:44210757-44210776 MS.gene050134:CDS 45.0%
!! AGGCTTTGAAGGAACCAGAA+AGG + chr4.2:44210608-44210627 MS.gene050134:CDS 45.0%
!! GGCTTTGAAGGAACCAGAAA+GGG + chr4.2:44210609-44210628 MS.gene050134:CDS 45.0%
AAGACCCTTCCAATCCTTGG+CGG - chr4.2:44210509-44210528 None:intergenic 50.0%
AGAAACCGCCAAGGATTGGA+AGG + chr4.2:44210501-44210520 MS.gene050134:CDS 50.0%
CCAAAGAAACCGCCAAGGAT+TGG + chr4.2:44210497-44210516 MS.gene050134:CDS 50.0%
CCAAGGATTGGAAGGGTCTT+AGG + chr4.2:44210509-44210528 MS.gene050134:CDS 50.0%
CCAATCCTTGGCGGTTTCTT+TGG - chr4.2:44210500-44210519 None:intergenic 50.0%
CCTAAGACCCTTCCAATCCT+TGG - chr4.2:44210512-44210531 None:intergenic 50.0%
CGTTCAGAATCGTCAGGCTA+AGG + chr4.2:44210549-44210568 MS.gene050134:CDS 50.0%
GAAACCGCCAAGGATTGGAA+GGG + chr4.2:44210502-44210521 MS.gene050134:CDS 50.0%
GATTATGAAGCCGAGGTCGA+TGG + chr4.2:44210702-44210721 MS.gene050134:CDS 50.0%
TTCTTTGGTGAGAGACCGAG+AGG - chr4.2:44210460-44210479 None:intergenic 50.0%
! CGGAACGGTGAAGGAGATTT+TGG + chr4.2:44210738-44210757 MS.gene050134:CDS 50.0%
AGACCGAGAGGACCGATCTT+GGG - chr4.2:44210448-44210467 None:intergenic 55.0%
AGTTCCCTCGCTCCCAAGAT+CGG + chr4.2:44210433-44210452 MS.gene050134:CDS 55.0%
CATCGCCAAAGAAACCGCCA+AGG + chr4.2:44210492-44210511 MS.gene050134:CDS 55.0%
GAAGTTTACGTCCGCGTCAC+CGG + chr4.2:44210391-44210410 MS.gene050134:CDS 55.0%
GCTCACCGTTCAGAATCGTC+AGG + chr4.2:44210543-44210562 MS.gene050134:CDS 55.0%
GTCTTCTTGCGGTCCCTTTC+TGG - chr4.2:44210625-44210644 None:intergenic 55.0%
! TGGGATGGATCGAGCTTTGG+CGG - chr4.2:44210364-44210383 None:intergenic 55.0%
AGCTCCTTCGCCATCGACCT+CGG - chr4.2:44210715-44210734 None:intergenic 60.0%
AGGACCGATCTTGGGAGCGA+GGG - chr4.2:44210440-44210459 None:intergenic 60.0%
GAGACCGAGAGGACCGATCT+TGG - chr4.2:44210449-44210468 None:intergenic 60.0%
GATCTTGGGAGCGAGGGAAC+TGG - chr4.2:44210434-44210453 None:intergenic 60.0%
GCTCCCAAGATCGGTCCTCT+CGG + chr4.2:44210442-44210461 MS.gene050134:CDS 60.0%
GGTCGCTGTTGTTCCGTCTG+CGG + chr4.2:44210570-44210589 MS.gene050134:CDS 60.0%
TCGATGGCGAAGGAGCTTGC+CGG + chr4.2:44210718-44210737 MS.gene050134:CDS 60.0%
ATAACCAGCGCCGCCGCAGA+CGG - chr4.2:44210586-44210605 None:intergenic 65.0%
CGCTGTTGTTCCGTCTGCGG+CGG + chr4.2:44210573-44210592 MS.gene050134:CDS 65.0%
GAAGCCGAGGTCGATGGCGA+AGG + chr4.2:44210708-44210727 MS.gene050134:CDS 65.0%
GAGGACCGATCTTGGGAGCG+AGG - chr4.2:44210441-44210460 None:intergenic 65.0%
GGAGCTTGCCGGAACGGTGA+AGG + chr4.2:44210729-44210748 MS.gene050134:CDS 65.0%
GGCGAAGGAGCTTGCCGGAA+CGG + chr4.2:44210723-44210742 MS.gene050134:CDS 65.0%
GTTTACGTCCGCGTCACCGG+CGG + chr4.2:44210394-44210413 MS.gene050134:CDS 65.0%
!! TGCGGCGGCGCTGGTTATCA+AGG + chr4.2:44210588-44210607 MS.gene050134:CDS 65.0%
CGACTTCACCGCCGGTGACG+CGG - chr4.2:44210405-44210424 None:intergenic 70.0%
CGCGTCACCGGCGGTGAAGT+CGG + chr4.2:44210403-44210422 MS.gene050134:CDS 70.0%
GGCAGCTCCGACTTCACCGC+CGG - chr4.2:44210413-44210432 None:intergenic 70.0%
TGTTCCGTCTGCGGCGGCGC+TGG + chr4.2:44210579-44210598 MS.gene050134:CDS 75.0%


Chromosome Type Strat End Strand Name
chr4.2 gene 44210358 44210858 44210358 ID=MS.gene050134
chr4.2 mRNA 44210358 44210858 44210358 ID=MS.gene050134.t1;Parent=MS.gene050134
chr4.2 exon 44210358 44210858 44210358 ID=MS.gene050134.t1.exon1;Parent=MS.gene050134.t1
chr4.2 CDS 44210358 44210858 44210358 ID=cds.MS.gene050134.t1;Parent=MS.gene050134.t1
Gene Sequence

>MS.gene050134

ATGCCGCCAAAGCTCGATCCATCCCAAATCGTCGAAGTTTACGTCCGCGTCACCGGCGGTGAAGTCGGAGCTGCCAGTTCCCTCGCTCCCAAGATCGGTCCTCTCGGTCTCTCACCAAAGAAGATCGGAGAAGACATCGCCAAAGAAACCGCCAAGGATTGGAAGGGTCTTAGGGTGACAGTCAAGCTCACCGTTCAGAATCGTCAGGCTAAGGTCGCTGTTGTTCCGTCTGCGGCGGCGCTGGTTATCAAGGCTTTGAAGGAACCAGAAAGGGACCGCAAGAAGACTAAGAACATCAAGCATAATGGGAATATCTCTCTTGATGATGTTATTGAGATTGCGAAGATTATGAAGCCGAGGTCGATGGCGAAGGAGCTTGCCGGAACGGTGAAGGAGATTTTGGGGACTTGTGTTTCTGTTGGGTGTACTGTTGATGGGAAAGATCCAAAGGATTTGCAGCAGGAGATTAGTGATGGTGATGTTGAGATTCCTCAGGATTGA

Protein sequence

>MS.gene050134.t1

MPPKLDPSQIVEVYVRVTGGEVGAASSLAPKIGPLGLSPKKIGEDIAKETAKDWKGLRVTVKLTVQNRQAKVAVVPSAAALVIKALKEPERDRKKTKNIKHNGNISLDDVIEIAKIMKPRSMAKELAGTVKEILGTCVSVGCTVDGKDPKDLQQEISDGDVEIPQD