Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51959.t1 | XP_024633784.1 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 6.40E-68 | 266.5 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51959.t1 | Q19AV6 | 33.6 | 116 | 73 | 3 | 15 | 129 | 20 | 132 | 1.9e-12 | 73.6 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51959.t1 | A0A396IIP6 | 99.2 | 132 | 1 | 0 | 1 | 132 | 1 | 132 | 4.6e-68 | 266.5 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049350 | MS.gene51959 | 0.810763 | 9.81E-51 | -1.69E-46 |
| MS.gene057102 | MS.gene51959 | 0.824707 | 6.98E-54 | -1.69E-46 |
| MS.gene061100 | MS.gene51959 | 0.803949 | 2.73E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51959.t1 | MTR_0393s0040 | 93.750 | 128 | 8 | 0 | 1 | 128 | 1 | 128 | 2.57e-89 | 255 |
| MS.gene51959.t1 | MTR_3g010320 | 93.182 | 132 | 8 | 1 | 1 | 132 | 1 | 131 | 1.37e-86 | 248 |
| MS.gene51959.t1 | MTR_8g072380 | 62.319 | 69 | 10 | 1 | 64 | 132 | 4 | 56 | 7.28e-22 | 82.4 |
| MS.gene51959.t1 | MTR_2g093710 | 46.729 | 107 | 20 | 3 | 52 | 126 | 7 | 108 | 2.78e-21 | 82.4 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene51959.t1 | AT4G33925 | 67.424 | 132 | 43 | 0 | 1 | 132 | 1 | 132 | 2.01e-64 | 192 |
| MS.gene51959.t1 | AT4G33925 | 66.387 | 119 | 40 | 0 | 1 | 119 | 1 | 119 | 5.82e-57 | 173 |
Find 23 sgRNAs with CRISPR-Local
Find 67 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TAGTGGAAGGTTTATCTTTC+AGG | 0.114697 | 3.2:+4065321 | MS.gene51959:CDS |
| ACTAGATTACATTTCTTATT+TGG | 0.150291 | 3.2:+4065232 | MS.gene51959:intron |
| TCTCCATTTGTCTAGGTTAC+AGG | 0.214028 | 3.2:+4066010 | MS.gene51959:intron |
| GAGAATGAGTGCAAGTAATT+TGG | 0.305236 | 3.2:+4064940 | None:intergenic |
| GCAAGACTCGCTGCATCATT+AGG | 0.352521 | 3.2:+4066282 | MS.gene51959:CDS |
| ATCATAGAAGAAAGAATAAC+AGG | 0.376490 | 3.2:-4066083 | None:intergenic |
| CAAATTGAATCTACACAAAC+TGG | 0.395068 | 3.2:+4064983 | MS.gene51959:CDS |
| CTCCATTTGTCTAGGTTACA+GGG | 0.399853 | 3.2:+4066011 | MS.gene51959:intron |
| TTTCAGCAAAACACAGATAC+TGG | 0.406479 | 3.2:-4066051 | None:intergenic |
| TCTAGGTTACAGGGGAATCC+AGG | 0.471736 | 3.2:+4066020 | MS.gene51959:intron |
| TGAGGGAGCTACTAGAATTG+TGG | 0.522452 | 3.2:+4065264 | MS.gene51959:CDS |
| TCTATGATGTTGTCAACAGA+GGG | 0.540121 | 3.2:+4066098 | MS.gene51959:CDS |
| TGAAAGATAAACCTTCCACT+AGG | 0.563754 | 3.2:-4065319 | None:intergenic |
| TTCTATGATGTTGTCAACAG+AGG | 0.568989 | 3.2:+4066097 | MS.gene51959:CDS |
| AAAATTTCTGCTGACCCTAG+TGG | 0.582691 | 3.2:+4065304 | MS.gene51959:CDS |
| AGGATCATACGTCGAAGTCA+AGG | 0.608072 | 3.2:+4066302 | MS.gene51959:CDS |
| TCCATTTGTCTAGGTTACAG+GGG | 0.612671 | 3.2:+4066012 | MS.gene51959:intron |
| CGAAGTCAAGGTATCTGATG+AGG | 0.612908 | 3.2:+4066314 | MS.gene51959:CDS |
| ATTTGGTAGCAGATCAAGTG+TGG | 0.614557 | 3.2:+4064957 | MS.gene51959:CDS |
| TTTCTGCTGACCCTAGTGGA+AGG | 0.616396 | 3.2:+4065308 | MS.gene51959:CDS |
| GAAAGATAAACCTTCCACTA+GGG | 0.628597 | 3.2:-4065318 | None:intergenic |
| ACTAGAATTGTGGATCAAAG+AGG | 0.644685 | 3.2:+4065274 | MS.gene51959:CDS |
| CTATGATGTTGTCAACAGAG+GGG | 0.760944 | 3.2:+4066099 | MS.gene51959:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATTGAAATTAAAGCAAAAAA+AGG | - | chr3.2:4065051-4065070 | None:intergenic | 15.0% |
| !! | TTGAAATTAAAGCAAAAAAA+GGG | - | chr3.2:4065050-4065069 | None:intergenic | 15.0% |
| !! | AAAACAAGAAAAGATGTAAA+AGG | - | chr3.2:4065154-4065173 | None:intergenic | 20.0% |
| !! | AAACAAACAAACAAAAACAA+AGG | - | chr3.2:4065380-4065399 | None:intergenic | 20.0% |
| !! | AAACAAGAAAAGATGTAAAA+GGG | - | chr3.2:4065153-4065172 | None:intergenic | 20.0% |
| !! | ACTAGATTACATTTCTTATT+TGG | + | chr3.2:4065232-4065251 | MS.gene51959:intron | 20.0% |
| !! | TTTCAACTATTCAATTGAAA+TGG | - | chr3.2:4065433-4065452 | None:intergenic | 20.0% |
| !!! | TTATTTGGTAAGAATTTTGA+GGG | + | chr3.2:4065247-4065266 | MS.gene51959:CDS | 20.0% |
| ! | ACTGTGACATGAAAATAAAA+AGG | - | chr3.2:4065094-4065113 | None:intergenic | 25.0% |
| ! | ATCATAGAAGAAAGAATAAC+AGG | - | chr3.2:4066086-4066105 | None:intergenic | 25.0% |
| ! | CAAATCACAATTTGAATCAA+GGG | - | chr3.2:4065985-4066004 | None:intergenic | 25.0% |
| ! | GAAGAAAATAAAAGACAACA+AGG | - | chr3.2:4066154-4066173 | None:intergenic | 25.0% |
| !! | CAAATTTTCATGACAGTATA+AGG | + | chr3.2:4065948-4065967 | MS.gene51959:intron | 25.0% |
| !! | CTTATTTGGTAAGAATTTTG+AGG | + | chr3.2:4065246-4065265 | MS.gene51959:CDS | 25.0% |
| !! | GCATTTTCTCAAATTTGTAT+AGG | + | chr3.2:4065834-4065853 | MS.gene51959:intron | 25.0% |
| !! | GTTTGACATTTTATGTTTGT+TGG | + | chr3.2:4065197-4065216 | MS.gene51959:intron | 25.0% |
| !!! | AAAATTGATTTTGGCATGTT+TGG | - | chr3.2:4065754-4065773 | None:intergenic | 25.0% |
| !!! | TAAGGGTGTAAAATTGATTT+TGG | - | chr3.2:4065763-4065782 | None:intergenic | 25.0% |
| !!! | TGTATGTTTGATTTCACTTT+TGG | - | chr3.2:4065804-4065823 | None:intergenic | 25.0% |
| !!! | TTCTTGTTTTTTTGTAGCAT+AGG | + | chr3.2:4065164-4065183 | MS.gene51959:intron | 25.0% |
| AAGGCAAAATCATTTCTACT+TGG | + | chr3.2:4065715-4065734 | MS.gene51959:intron | 30.0% | |
| AGGCAAAATCATTTCTACTT+GGG | + | chr3.2:4065716-4065735 | MS.gene51959:intron | 30.0% | |
| ATTGTCTTCTTCATTCAACA+GGG | + | chr3.2:4065405-4065424 | MS.gene51959:intron | 30.0% | |
| CAAATTGAATCTACACAAAC+TGG | + | chr3.2:4064983-4065002 | MS.gene51959:CDS | 30.0% | |
| CAAGTTAGAATCAATTCTGA+AGG | + | chr3.2:4065696-4065715 | MS.gene51959:intron | 30.0% | |
| GCAAATCACAATTTGAATCA+AGG | - | chr3.2:4065986-4066005 | None:intergenic | 30.0% | |
| TATTGTCTTCTTCATTCAAC+AGG | + | chr3.2:4065404-4065423 | MS.gene51959:intron | 30.0% | |
| ! | AAAAAGGTGCAACAAAAGTT+AGG | - | chr3.2:4065078-4065097 | None:intergenic | 30.0% |
| ! | AAAAGGTGCAACAAAAGTTA+GGG | - | chr3.2:4065077-4065096 | None:intergenic | 30.0% |
| !! | AGGAGTCAAAATTGTTTCTA+AGG | - | chr3.2:4065781-4065800 | None:intergenic | 30.0% |
| !! | ATGTTTGATTTCACTTTTGG+AGG | - | chr3.2:4065801-4065820 | None:intergenic | 30.0% |
| !! | GGAGTCAAAATTGTTTCTAA+GGG | - | chr3.2:4065780-4065799 | None:intergenic | 30.0% |
| AATCATTTCTACTTGGGACT+TGG | + | chr3.2:4065722-4065741 | MS.gene51959:intron | 35.0% | |
| ACTAGAATTGTGGATCAAAG+AGG | + | chr3.2:4065274-4065293 | MS.gene51959:CDS | 35.0% | |
| ATCATTTCTACTTGGGACTT+GGG | + | chr3.2:4065723-4065742 | MS.gene51959:intron | 35.0% | |
| GAAAGATAAACCTTCCACTA+GGG | - | chr3.2:4065321-4065340 | None:intergenic | 35.0% | |
| GTGTCAAACACATACATATG+TGG | + | chr3.2:4065564-4065583 | MS.gene51959:intron | 35.0% | |
| TAGTGGAAGGTTTATCTTTC+AGG | + | chr3.2:4065321-4065340 | MS.gene51959:CDS | 35.0% | |
| TCTATGATGTTGTCAACAGA+GGG | + | chr3.2:4066098-4066117 | MS.gene51959:CDS | 35.0% | |
| TGAAAGATAAACCTTCCACT+AGG | - | chr3.2:4065322-4065341 | None:intergenic | 35.0% | |
| TGCATATTCTCCATTTGTCT+AGG | + | chr3.2:4066003-4066022 | MS.gene51959:intron | 35.0% | |
| TTCTATGATGTTGTCAACAG+AGG | + | chr3.2:4066097-4066116 | MS.gene51959:CDS | 35.0% | |
| TTGTCTTCTTCATTCAACAG+GGG | + | chr3.2:4065406-4065425 | MS.gene51959:intron | 35.0% | |
| TTTCAGCAAAACACAGATAC+TGG | - | chr3.2:4066054-4066073 | None:intergenic | 35.0% | |
| ! | AGACAACAAGGAAGTGTATA+TGG | - | chr3.2:4066142-4066161 | None:intergenic | 35.0% |
| ! | ATGCCTTCAATCTAAAGTGT+CGG | - | chr3.2:4065527-4065546 | None:intergenic | 35.0% |
| ! | CATTGGTTGCATCTAATTGT+TGG | + | chr3.2:4065897-4065916 | MS.gene51959:intron | 35.0% |
| AAAATTTCTGCTGACCCTAG+TGG | + | chr3.2:4065304-4065323 | MS.gene51959:CDS | 40.0% | |
| CATGTCTGTGTTTGCTTCAT+TGG | + | chr3.2:4065880-4065899 | MS.gene51959:intron | 40.0% | |
| CTATGATGTTGTCAACAGAG+GGG | + | chr3.2:4066099-4066118 | MS.gene51959:CDS | 40.0% | |
| CTCCATTTGTCTAGGTTACA+GGG | + | chr3.2:4066011-4066030 | MS.gene51959:intron | 40.0% | |
| TCCATTTGTCTAGGTTACAG+GGG | + | chr3.2:4066012-4066031 | MS.gene51959:intron | 40.0% | |
| TCTCCATTTGTCTAGGTTAC+AGG | + | chr3.2:4066010-4066029 | MS.gene51959:intron | 40.0% | |
| ! | ATTTGGTAGCAGATCAAGTG+TGG | + | chr3.2:4064957-4064976 | MS.gene51959:CDS | 40.0% |
| !!! | CAGATACTGGTCTTTTTTCC+TGG | - | chr3.2:4066041-4066060 | None:intergenic | 40.0% |
| AGGATCATACGTCGAAGTCA+AGG | + | chr3.2:4066302-4066321 | MS.gene51959:CDS | 45.0% | |
| GGCATGTCTGATATCCAACA+CGG | + | chr3.2:4065542-4065561 | MS.gene51959:intron | 45.0% | |
| GTATGTGTTTGACACCGTGT+TGG | - | chr3.2:4065559-4065578 | None:intergenic | 45.0% | |
| TCCCCTGTAACCTAGACAAA+TGG | - | chr3.2:4066016-4066035 | None:intergenic | 45.0% | |
| TGAGGGAGCTACTAGAATTG+TGG | + | chr3.2:4065264-4065283 | MS.gene51959:CDS | 45.0% | |
| ! | CGAAGTCAAGGTATCTGATG+AGG | + | chr3.2:4066314-4066333 | MS.gene51959:CDS | 45.0% |
| ! | GCACCGACACTTTAGATTGA+AGG | + | chr3.2:4065521-4065540 | MS.gene51959:intron | 45.0% |
| !! | TTAAAATATATTAATATTGT+TGG | + | chr3.2:4065920-4065939 | MS.gene51959:intron | 5.0% |
| GCAAGACTCGCTGCATCATT+AGG | + | chr3.2:4066282-4066301 | MS.gene51959:CDS | 50.0% | |
| TCTAGGTTACAGGGGAATCC+AGG | + | chr3.2:4066020-4066039 | MS.gene51959:intron | 50.0% | |
| TTTCTGCTGACCCTAGTGGA+AGG | + | chr3.2:4065308-4065327 | MS.gene51959:CDS | 50.0% | |
| GAGGGGAGCAACTCTGTGTA+AGG | + | chr3.2:4066116-4066135 | MS.gene51959:intron | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.2 | gene | 4064944 | 4066365 | 4064944 | ID=MS.gene51959 |
| chr3.2 | mRNA | 4064944 | 4066365 | 4064944 | ID=MS.gene51959.t1;Parent=MS.gene51959 |
| chr3.2 | exon | 4064944 | 4065004 | 4064944 | ID=MS.gene51959.t1.exon1;Parent=MS.gene51959.t1 |
| chr3.2 | CDS | 4064944 | 4065004 | 4064944 | ID=cds.MS.gene51959.t1;Parent=MS.gene51959.t1 |
| chr3.2 | exon | 4065113 | 4065134 | 4065113 | ID=MS.gene51959.t1.exon2;Parent=MS.gene51959.t1 |
| chr3.2 | CDS | 4065113 | 4065134 | 4065113 | ID=cds.MS.gene51959.t1;Parent=MS.gene51959.t1 |
| chr3.2 | exon | 4065237 | 4065342 | 4065237 | ID=MS.gene51959.t1.exon3;Parent=MS.gene51959.t1 |
| chr3.2 | CDS | 4065237 | 4065342 | 4065237 | ID=cds.MS.gene51959.t1;Parent=MS.gene51959.t1 |
| chr3.2 | exon | 4066025 | 4066132 | 4066025 | ID=MS.gene51959.t1.exon4;Parent=MS.gene51959.t1 |
| chr3.2 | CDS | 4066025 | 4066132 | 4066025 | ID=cds.MS.gene51959.t1;Parent=MS.gene51959.t1 |
| chr3.2 | exon | 4066264 | 4066365 | 4066264 | ID=MS.gene51959.t1.exon5;Parent=MS.gene51959.t1 |
| chr3.2 | CDS | 4066264 | 4066365 | 4066264 | ID=cds.MS.gene51959.t1;Parent=MS.gene51959.t1 |
| Gene Sequence |
| Protein sequence |
