Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52199.t1 | XP_003623880.1 | 100 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 3.20E-28 | 134 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52199.t1 | G7L2K4 | 100.0 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 2.3e-28 | 134.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049808 | MS.gene52199 | 0.839901 | 1.20E-57 | -1.69E-46 |
| MS.gene050237 | MS.gene52199 | 0.821908 | 3.14E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene52199 | 0.815859 | 7.45E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene52199 | 0.810714 | 1.00E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52199.t1 | MTR_7g076630 | 100.000 | 62 | 0 | 0 | 1 | 62 | 1 | 62 | 5.09e-41 | 129 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52199.t1 | AT4G00585 | 83.051 | 59 | 9 | 1 | 4 | 62 | 2 | 59 | 1.71e-29 | 100 |
Find 23 sgRNAs with CRISPR-Local
Find 47 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GTGTTGCGTTTCCAGTGTTT+AGG | 0.292140 | 7.4:+29047345 | None:intergenic |
| GAGCCAATGGAGGAGATTTC+AGG | 0.296342 | 7.4:-29047407 | MS.gene52199:CDS |
| TGTTGCGTTTCCAGTGTTTA+GGG | 0.348623 | 7.4:+29047346 | None:intergenic |
| GAGATTTCAGGTACAAGGTT+TGG | 0.375661 | 7.4:-29047395 | MS.gene52199:CDS |
| TTTAGCTGAGAGTTTGAAGA+TGG | 0.461581 | 7.4:+29047284 | None:intergenic |
| CACGGTAATGGCGGAGCCAA+TGG | 0.484941 | 7.4:-29047420 | MS.gene52199:CDS |
| TAGCTGAGAGTTTGAAGATG+GGG | 0.490985 | 7.4:+29047286 | None:intergenic |
| GTACCTGAAATCTCCTCCAT+TGG | 0.492681 | 7.4:+29047404 | None:intergenic |
| AACACTGCCATCGCTATGTT+CGG | 0.493133 | 7.4:-29047327 | MS.gene52199:CDS |
| TGGAGGAGATTTCAGGTACA+AGG | 0.512900 | 7.4:-29047400 | MS.gene52199:CDS |
| TTAGCTGAGAGTTTGAAGAT+GGG | 0.520876 | 7.4:+29047285 | None:intergenic |
| GGTGGAGCAGATCACGGTAA+TGG | 0.524664 | 7.4:-29047432 | MS.gene52199:CDS |
| TACAAGGTTTGGAGTATGAC+TGG | 0.533279 | 7.4:-29047384 | MS.gene52199:CDS |
| CTCACTGTCGCCCTAAACAC+TGG | 0.552111 | 7.4:-29047356 | MS.gene52199:CDS |
| CAAACTCTCAGCTAAACTTG+AGG | 0.608144 | 7.4:-29047277 | MS.gene52199:intron |
| TGGTATCCAATCCCATGCAT+CGG | 0.619221 | 7.4:+29046813 | None:intergenic |
| GGAGGTGGTGGAGCAGATCA+CGG | 0.622572 | 7.4:-29047438 | MS.gene52199:CDS |
| GGTAATGGCGGAGCCAATGG+AGG | 0.641548 | 7.4:-29047417 | MS.gene52199:CDS |
| GACGATACCGAACATAGCGA+TGG | 0.647243 | 7.4:+29047320 | None:intergenic |
| GGAGCAGATCACGGTAATGG+CGG | 0.651187 | 7.4:-29047429 | MS.gene52199:CDS |
| CAGTGTTTAGGGCGACAGTG+AGG | 0.708119 | 7.4:+29047357 | None:intergenic |
| AAGGTTTGGAGTATGACTGG+TGG | 0.712549 | 7.4:-29047381 | MS.gene52199:CDS |
| TGAAGATGGGGATACAAACG+AGG | 0.746465 | 7.4:+29047298 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TTATTGTTGTTATTATTATT+TGG | - | chr7.4:29047201-29047220 | MS.gene52199:intron | 10.0% |
| !!! | GATTTTGTGTTTAGAAAATA+TGG | - | chr7.4:29047355-29047374 | MS.gene52199:CDS | 20.0% |
| ! | AAAATCAAAACATGGAAATC+TGG | + | chr7.4:29047042-29047061 | None:intergenic | 25.0% |
| ! | CAATGTTTACTTTGATACAT+TGG | - | chr7.4:29047294-29047313 | MS.gene52199:CDS | 25.0% |
| ! | GGAGAAATAAAATCAAAACA+TGG | + | chr7.4:29047050-29047069 | None:intergenic | 25.0% |
| !! | TAACGGAATCTTAATTTGAT+TGG | - | chr7.4:29047172-29047191 | MS.gene52199:intron | 25.0% |
| !!! | TAGCGACAATTTTAATCAAA+GGG | - | chr7.4:29047400-29047419 | MS.gene52199:CDS | 25.0% |
| ATAAATGAGGGACTTGATAA+CGG | - | chr7.4:29047155-29047174 | MS.gene52199:intron | 30.0% | |
| ! | TTTTCGTTGCGAATAAATGA+GGG | - | chr7.4:29047143-29047162 | MS.gene52199:intron | 30.0% |
| !! | AGCGACAATTTTAATCAAAG+GGG | - | chr7.4:29047401-29047420 | MS.gene52199:CDS | 30.0% |
| !! | GTAGCGACAATTTTAATCAA+AGG | - | chr7.4:29047399-29047418 | MS.gene52199:CDS | 30.0% |
| GGAAATCTGGTAAAGAAAAC+AGG | + | chr7.4:29047029-29047048 | None:intergenic | 35.0% | |
| GTTAATGTTAACGACGATCT+GGG | - | chr7.4:29047256-29047275 | MS.gene52199:intron | 35.0% | |
| TGTTAATGTTAACGACGATC+TGG | - | chr7.4:29047255-29047274 | MS.gene52199:intron | 35.0% | |
| TTAGCTGAGAGTTTGAAGAT+GGG | + | chr7.4:29046969-29046988 | None:intergenic | 35.0% | |
| TTTAGCTGAGAGTTTGAAGA+TGG | + | chr7.4:29046970-29046989 | None:intergenic | 35.0% | |
| ! | GGCGAAATTGAGAATGTTTT+GGG | - | chr7.4:29047222-29047241 | MS.gene52199:intron | 35.0% |
| ! | GTTTTCGTTGCGAATAAATG+AGG | - | chr7.4:29047142-29047161 | MS.gene52199:intron | 35.0% |
| ! | TGATACATTGGTTAGTATGC+TGG | - | chr7.4:29047306-29047325 | MS.gene52199:CDS | 35.0% |
| ! | TGGCGAAATTGAGAATGTTT+TGG | - | chr7.4:29047221-29047240 | MS.gene52199:intron | 35.0% |
| CAAACTCTCAGCTAAACTTG+AGG | - | chr7.4:29046974-29046993 | MS.gene52199:intron | 40.0% | |
| TAGCTGAGAGTTTGAAGATG+GGG | + | chr7.4:29046968-29046987 | None:intergenic | 40.0% | |
| TGTTGCGTTTCCAGTGTTTA+GGG | + | chr7.4:29046908-29046927 | None:intergenic | 40.0% | |
| ! | GAGATTTCAGGTACAAGGTT+TGG | - | chr7.4:29046856-29046875 | MS.gene52199:CDS | 40.0% |
| ! | TACAAGGTTTGGAGTATGAC+TGG | - | chr7.4:29046867-29046886 | MS.gene52199:intron | 40.0% |
| ! | TGAGATTTTCACCGATGCAT+GGG | - | chr7.4:29047427-29047446 | MS.gene52199:CDS | 40.0% |
| AACACTGCCATCGCTATGTT+CGG | - | chr7.4:29046924-29046943 | MS.gene52199:intron | 45.0% | |
| GTACCTGAAATCTCCTCCAT+TGG | + | chr7.4:29046850-29046869 | None:intergenic | 45.0% | |
| GTGTTGCGTTTCCAGTGTTT+AGG | + | chr7.4:29046909-29046928 | None:intergenic | 45.0% | |
| TGAAGATGGGGATACAAACG+AGG | + | chr7.4:29046956-29046975 | None:intergenic | 45.0% | |
| TGGTATCCAATCCCATGCAT+CGG | + | chr7.4:29047441-29047460 | None:intergenic | 45.0% | |
| ! | AAGGTTTGGAGTATGACTGG+TGG | - | chr7.4:29046870-29046889 | MS.gene52199:intron | 45.0% |
| ! | GTGAGATTTTCACCGATGCA+TGG | - | chr7.4:29047426-29047445 | MS.gene52199:CDS | 45.0% |
| ! | TGGAGGAGATTTCAGGTACA+AGG | - | chr7.4:29046851-29046870 | MS.gene52199:CDS | 45.0% |
| ! | TTTTCACCGATGCATGGGAT+TGG | - | chr7.4:29047432-29047451 | MS.gene52199:CDS | 45.0% |
| !!! | AAAAAAAATTGATAATAATT+TGG | + | chr7.4:29047071-29047090 | None:intergenic | 5.0% |
| !!! | AATTATTATCAATTTTTTTT+AGG | - | chr7.4:29047071-29047090 | MS.gene52199:intron | 5.0% |
| !!! | ATTATTATCAATTTTTTTTA+GGG | - | chr7.4:29047072-29047091 | MS.gene52199:intron | 5.0% |
| GACGATACCGAACATAGCGA+TGG | + | chr7.4:29046934-29046953 | None:intergenic | 50.0% | |
| GAGCCAATGGAGGAGATTTC+AGG | - | chr7.4:29046844-29046863 | MS.gene52199:CDS | 50.0% | |
| CTCACTGTCGCCCTAAACAC+TGG | - | chr7.4:29046895-29046914 | MS.gene52199:intron | 55.0% | |
| GGTGGAGCAGATCACGGTAA+TGG | - | chr7.4:29046819-29046838 | MS.gene52199:CDS | 55.0% | |
| ! | CAGTGTTTAGGGCGACAGTG+AGG | + | chr7.4:29046897-29046916 | None:intergenic | 55.0% |
| ! | GGAGCAGATCACGGTAATGG+CGG | - | chr7.4:29046822-29046841 | MS.gene52199:CDS | 55.0% |
| ! | CACGGTAATGGCGGAGCCAA+TGG | - | chr7.4:29046831-29046850 | MS.gene52199:CDS | 60.0% |
| ! | GGTAATGGCGGAGCCAATGG+AGG | - | chr7.4:29046834-29046853 | MS.gene52199:CDS | 60.0% |
| !! | GGAGGTGGTGGAGCAGATCA+CGG | - | chr7.4:29046813-29046832 | MS.gene52199:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 29046810 | 29047463 | 29046810 | ID=MS.gene52199 |
| chr7.4 | mRNA | 29046810 | 29047463 | 29046810 | ID=MS.gene52199.t1;Parent=MS.gene52199 |
| chr7.4 | exon | 29047278 | 29047463 | 29047278 | ID=MS.gene52199.t1.exon1;Parent=MS.gene52199.t1 |
| chr7.4 | CDS | 29047278 | 29047463 | 29047278 | ID=cds.MS.gene52199.t1;Parent=MS.gene52199.t1 |
| chr7.4 | exon | 29046810 | 29046881 | 29046810 | ID=MS.gene52199.t1.exon2;Parent=MS.gene52199.t1 |
| chr7.4 | CDS | 29046810 | 29046881 | 29046810 | ID=cds.MS.gene52199.t1;Parent=MS.gene52199.t1 |
| Gene Sequence |
| Protein sequence |
