AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene049808


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049808.t1 MTR_2g020770 99.346 153 1 0 1 153 1 153 5.93e-111 311
MS.gene049808.t1 MTR_2g020770 96.429 140 5 0 1 140 1 140 2.35e-97 277
MS.gene049808.t1 MTR_1g082290 56.835 139 60 0 5 143 6 144 1.09e-59 182
MS.gene049808.t1 MTR_7g114020 60.993 141 54 1 9 148 6 146 4.28e-59 180
MS.gene049808.t1 MTR_1g089130 55.944 143 63 0 5 147 3 145 1.06e-56 174
MS.gene049808.t1 MTR_2g100880 42.446 139 80 0 1 139 1 139 5.20e-39 129
MS.gene049808.t1 MTR_2g100900 42.446 139 80 0 1 139 1 139 9.47e-39 129
MS.gene049808.t1 MTR_2g103870 46.207 145 73 3 10 149 10 154 1.79e-31 110
MS.gene049808.t1 MTR_4g010160 43.478 115 63 2 37 149 28 142 7.56e-28 101
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene049808.t1 AT3G21510 70.588 153 45 0 1 153 1 153 6.73e-71 210
MS.gene049808.t1 AT1G03430 52.632 152 71 1 3 153 6 157 7.81e-56 172
MS.gene049808.t1 AT5G39340 50.331 151 74 1 3 152 5 155 3.76e-51 160
MS.gene049808.t1 AT3G29350 48.026 152 78 1 3 153 5 156 4.67e-51 160
MS.gene049808.t1 AT5G39340 50.331 151 74 1 3 152 38 188 1.44e-50 160
MS.gene049808.t1 AT5G39340 50.331 151 74 1 3 152 54 204 3.23e-50 160
MS.gene049808.t1 AT5G39340 50.331 151 74 1 3 152 54 204 3.23e-50 160
MS.gene049808.t1 AT1G03430 51.852 135 64 1 3 136 6 140 1.82e-46 149
MS.gene049808.t1 AT3G16360 44.030 134 75 0 6 139 1 134 8.65e-39 129
MS.gene049808.t1 AT3G16360 45.000 120 66 0 19 138 8 127 1.04e-36 123
MS.gene049808.t1 AT3G29350 47.273 110 57 1 3 111 5 114 6.43e-34 115
MS.gene049808.t1 AT1G80100 49.275 138 68 2 14 149 17 154 2.32e-27 100
MS.gene049808.t1 AT1G80100 47.778 90 46 1 61 149 43 132 1.21e-21 84.7
MS.gene049808.t1 AT4G04402 33.750 160 94 4 6 153 8 167 1.32e-21 85.9
MS.gene049808.t1 AT1G80100 46.774 124 65 1 14 136 17 140 1.51e-21 85.5

Find 29 sgRNAs with CRISPR-Local

Find 78 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TGCACATGTTCACCAGTTTA+AGG 0.220882 2.2:+64473491 MS.gene049808:CDS
ACTCAGTTCAGTTCCATCAT+AGG 0.355461 2.2:-64474216 None:intergenic
GTGTTGAGAAAAGGGTTTCT+TGG 0.384266 2.2:+64473022 MS.gene049808:intron
GCTGCTGAAGTTGCAGAAAC+TGG 0.419928 2.2:-64473049 None:intergenic
TTGCACTGCTACCCTTAAAC+TGG 0.425560 2.2:-64473503 None:intergenic
GCACATGTTCACCAGTTTAA+GGG 0.428803 2.2:+64473492 MS.gene049808:CDS
CTACATCAAATCCATGTTCA+TGG 0.459422 2.2:+64472872 MS.gene049808:CDS
CTAATTTATGCAGGTGCCGC+AGG 0.459692 2.2:+64473999 MS.gene049808:intron
GCAGCTTGAGCAGCAGATAG+TGG 0.473916 2.2:+64474176 MS.gene049808:intron
CTCAGTTCAGTTCCATCATA+GGG 0.476361 2.2:-64474215 None:intergenic
TGAGAAAAGGGTTTCTTGGA+TGG 0.497354 2.2:+64473026 MS.gene049808:intron
GGGTCAGATGCGGAGACAGT+GGG 0.506261 2.2:+64472845 MS.gene049808:CDS
TAAGCTTGAAACATTGTTGA+GGG 0.522076 2.2:+64474063 MS.gene049808:CDS
TGACAGCATAGGTGCACAAA+GGG 0.524943 2.2:+64473750 MS.gene049808:intron
GAGCAGCAGATAGTGGCAGC+TGG 0.537850 2.2:+64474183 MS.gene049808:CDS
TGGTCTAACCTCCATGAACA+TGG 0.544818 2.2:-64472883 None:intergenic
TGGTGGCTCAATCCCTATGA+TGG 0.558041 2.2:+64474203 MS.gene049808:CDS
TTTGTAGAGAAACATGGAGG+TGG 0.564605 2.2:+64472824 None:intergenic
ATAAGCTTGAAACATTGTTG+AGG 0.584829 2.2:+64474062 MS.gene049808:CDS
TGGGTCAGATGCGGAGACAG+TGG 0.592973 2.2:+64472844 MS.gene049808:CDS
TCAGATGCGGAGACAGTGGG+TGG 0.608143 2.2:+64472848 MS.gene049808:CDS
TTGTAGAGAAACATGGAGGT+GGG 0.611758 2.2:+64472825 None:intergenic
AGTTTAAGGGTAGCAGTGCA+AGG 0.619461 2.2:+64473505 MS.gene049808:CDS
TCACTTGCTGCAAACACCTG+CGG 0.622315 2.2:-64474015 None:intergenic
CAGCAGATAGTGGCAGCTGG+TGG 0.634128 2.2:+64474186 MS.gene049808:CDS
GAAATTGCGAAAAGCAACGC+AGG 0.637314 2.2:-64473782 None:intergenic
ACATGGAGGTGGGTCAGATG+CGG 0.639294 2.2:+64472835 None:intergenic
TTGACAGCATAGGTGCACAA+AGG 0.666337 2.2:+64473749 MS.gene049808:intron
CATCAAATCCATGTTCATGG+AGG 0.716249 2.2:+64472875 MS.gene049808:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AATTTATATGATTTCTTTTA+TGG + chr2.2:64474144-64474163 MS.gene049808:intron 10.0%
!! AAATCCATAAGTTTAATATA+AGG - chr2.2:64474122-64474141 None:intergenic 15.0%
!! ATTAGAGAATATAATGAATT+TGG + chr2.2:64473219-64473238 MS.gene049808:intron 15.0%
!!! ACTATGCTATTATATTATTT+TGG + chr2.2:64473699-64473718 MS.gene049808:intron 15.0%
!! AAATAGTCTGTTATGAAATT+CGG - chr2.2:64473659-64473678 None:intergenic 20.0%
!! AGAACCTTATATTAAACTTA+TGG + chr2.2:64474115-64474134 MS.gene049808:intron 20.0%
!!! CTGTAGCATTTATAAATTTT+AGG - chr2.2:64473553-64473572 None:intergenic 20.0%
!!! TTTTCAATTGTTGATTTTGT+TGG + chr2.2:64472978-64472997 MS.gene049808:intron 20.0%
!!! TTTTGCTTTTGTTTTTGTTT+AGG + chr2.2:64472945-64472964 MS.gene049808:intron 20.0%
! AACATGAGAGAAATGAAAAA+AGG - chr2.2:64473195-64473214 None:intergenic 25.0%
! ACAACTTCAAAAACAAATTC+AGG - chr2.2:64473092-64473111 None:intergenic 25.0%
! AGTAAAGTATAACCAAGAAT+TGG - chr2.2:64473313-64473332 None:intergenic 25.0%
! CAACTTCAAAAACAAATTCA+GGG - chr2.2:64473091-64473110 None:intergenic 25.0%
! GTGAAATTTCTAATTTATGC+AGG + chr2.2:64473990-64474009 MS.gene049808:intron 25.0%
!! AGCATTTATAAATTTTAGGC+TGG - chr2.2:64473549-64473568 None:intergenic 25.0%
!! CATGATCTTTAATGTTGTTT+GGG + chr2.2:64473421-64473440 MS.gene049808:intron 25.0%
!! TGAATTTTTCGCTAATTTCA+TGG + chr2.2:64473958-64473977 MS.gene049808:intron 25.0%
!!! GACTAGTTGTTTAATAGTTT+TGG + chr2.2:64473846-64473865 MS.gene049808:intron 25.0%
!!! TTTTGTTTGTGTTGAGAAAA+GGG + chr2.2:64473014-64473033 MS.gene049808:intron 25.0%
!!! TTTTTTTGCTATTAATGACC+CGG - chr2.2:64473381-64473400 None:intergenic 25.0%
ATAAGCTTGAAACATTGTTG+AGG + chr2.2:64474062-64474081 MS.gene049808:CDS 30.0%
GCATGATCTTTAATGTTGTT+TGG + chr2.2:64473420-64473439 MS.gene049808:intron 30.0%
GCTACAGAATCTGAATAAAT+GGG + chr2.2:64473566-64473585 MS.gene049808:intron 30.0%
TAAGCTTGAAACATTGTTGA+GGG + chr2.2:64474063-64474082 MS.gene049808:CDS 30.0%
TGCTACAGAATCTGAATAAA+TGG + chr2.2:64473565-64473584 MS.gene049808:intron 30.0%
! ACACATTAATTGACTCTTCT+GGG + chr2.2:64472912-64472931 MS.gene049808:intron 30.0%
! GTCAATTAATGTGTGTTTTG+TGG - chr2.2:64472906-64472925 None:intergenic 30.0%
!! TTTTCTTTGATGATTCTGAG+AGG + chr2.2:64473117-64473136 MS.gene049808:CDS 30.0%
!!! GTTTTGTTTGTGTTGAGAAA+AGG + chr2.2:64473013-64473032 MS.gene049808:intron 30.0%
AATAGCTACACAAGAATTGC+AGG - chr2.2:64473608-64473627 None:intergenic 35.0%
CTACATCAAATCCATGTTCA+TGG + chr2.2:64472872-64472891 MS.gene049808:CDS 35.0%
GTATGTTTGATTGACAGCAT+AGG + chr2.2:64473739-64473758 MS.gene049808:intron 35.0%
! CACACATTAATTGACTCTTC+TGG + chr2.2:64472911-64472930 MS.gene049808:intron 35.0%
CAGCTAGTGCAATAGTTTGA+AGG + chr2.2:64473885-64473904 MS.gene049808:intron 40.0%
CATCAAATCCATGTTCATGG+AGG + chr2.2:64472875-64472894 MS.gene049808:CDS 40.0%
CTTAACTGCTTAGATGCTAG+AGG + chr2.2:64473927-64473946 MS.gene049808:intron 40.0%
GCACATGTTCACCAGTTTAA+GGG + chr2.2:64473492-64473511 MS.gene049808:CDS 40.0%
GTGTTGAGAAAAGGGTTTCT+TGG + chr2.2:64473022-64473041 MS.gene049808:intron 40.0%
TATTAATGACCCGGTTCCTA+GGG - chr2.2:64473372-64473391 None:intergenic 40.0%
TGAGAAAAGGGTTTCTTGGA+TGG + chr2.2:64473026-64473045 MS.gene049808:intron 40.0%
TGCACATGTTCACCAGTTTA+AGG + chr2.2:64473491-64473510 MS.gene049808:CDS 40.0%
!! GTTTGGGTTTTACATGTGCA+GGG + chr2.2:64473437-64473456 MS.gene049808:intron 40.0%
!! TGTTTGGGTTTTACATGTGC+AGG + chr2.2:64473436-64473455 MS.gene049808:intron 40.0%
AGTTTAAGGGTAGCAGTGCA+AGG + chr2.2:64473505-64473524 MS.gene049808:CDS 45.0%
CTATTAATGACCCGGTTCCT+AGG - chr2.2:64473373-64473392 None:intergenic 45.0%
CTTACCTCTCGAGTGAACTT+CGG + chr2.2:64473331-64473350 MS.gene049808:intron 45.0%
GAAATTGCGAAAAGCAACGC+AGG - chr2.2:64473785-64473804 None:intergenic 45.0%
TAATCCGAAGTTCACTCGAG+AGG - chr2.2:64473338-64473357 None:intergenic 45.0%
TGACAGCATAGGTGCACAAA+GGG + chr2.2:64473750-64473769 MS.gene049808:intron 45.0%
TGGTCTAACCTCCATGAACA+TGG - chr2.2:64472886-64472905 None:intergenic 45.0%
TTGACAGCATAGGTGCACAA+AGG + chr2.2:64473749-64473768 MS.gene049808:intron 45.0%
TTGCACTGCTACCCTTAAAC+TGG - chr2.2:64473506-64473525 None:intergenic 45.0%
AACCAAGAATTGGTCCACCG+AGG - chr2.2:64473303-64473322 None:intergenic 50.0%
ATCCTCGGTGGACCAATTCT+TGG + chr2.2:64473298-64473317 MS.gene049808:intron 50.0%
CCGAGGATAGTTCTCCTGAA+CGG - chr2.2:64473286-64473305 None:intergenic 50.0%
CCGTTCAGGAGAACTATCCT+CGG + chr2.2:64473283-64473302 MS.gene049808:intron 50.0%
CGAGTGAACTTCGGATTACC+AGG + chr2.2:64473340-64473359 MS.gene049808:intron 50.0%
GCTGCTGAAGTTGCAGAAAC+TGG - chr2.2:64473052-64473071 None:intergenic 50.0%
TCTCCTGAACGGTTCGTTCT+AGG - chr2.2:64473275-64473294 None:intergenic 50.0%
TGGTGGCTCAATCCCTATGA+TGG + chr2.2:64474203-64474222 MS.gene049808:CDS 50.0%
TTCAGGAGAACTATCCTCGG+TGG + chr2.2:64473286-64473305 MS.gene049808:intron 50.0%
! CTAATTTATGCAGGTGCCGC+AGG + chr2.2:64473999-64474018 MS.gene049808:intron 50.0%
! TCACTTGCTGCAAACACCTG+CGG - chr2.2:64474018-64474037 None:intergenic 50.0%
AATGACCCGGTTCCTAGGGA+AGG - chr2.2:64473368-64473387 None:intergenic 55.0%
ATGACCCGGTTCCTAGGGAA+GGG - chr2.2:64473367-64473386 None:intergenic 55.0%
CCACCTAGAACGAACCGTTC+AGG + chr2.2:64473269-64473288 MS.gene049808:intron 55.0%
GCAGCTTGAGCAGCAGATAG+TGG + chr2.2:64474176-64474195 MS.gene049808:intron 55.0%
! CCTGAACGGTTCGTTCTAGG+TGG - chr2.2:64473272-64473291 None:intergenic 55.0%
GATTACCAGGCCCCTTCCCT+AGG + chr2.2:64473353-64473372 MS.gene049808:intron 60.0%
GGGTCAGATGCGGAGACAGT+GGG + chr2.2:64472845-64472864 MS.gene049808:CDS 60.0%
TCAGATGCGGAGACAGTGGG+TGG + chr2.2:64472848-64472867 MS.gene049808:CDS 60.0%
TGACCCGGTTCCTAGGGAAG+GGG - chr2.2:64473366-64473385 None:intergenic 60.0%
TGGGTCAGATGCGGAGACAG+TGG + chr2.2:64472844-64472863 MS.gene049808:CDS 60.0%
! CAGCAGATAGTGGCAGCTGG+TGG + chr2.2:64474186-64474205 MS.gene049808:CDS 60.0%
! GAGCAGCAGATAGTGGCAGC+TGG + chr2.2:64474183-64474202 MS.gene049808:CDS 60.0%
AGGCCCCTTCCCTAGGAACC+GGG + chr2.2:64473360-64473379 MS.gene049808:intron 65.0%
CAGGCCCCTTCCCTAGGAAC+CGG + chr2.2:64473359-64473378 MS.gene049808:intron 65.0%
CGGTTCCTAGGGAAGGGGCC+TGG - chr2.2:64473361-64473380 None:intergenic 70.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 64472837 64474236 64472837 ID=MS.gene049808
chr2.2 mRNA 64472837 64474236 64472837 ID=MS.gene049808.t1;Parent=MS.gene049808
chr2.2 exon 64472837 64472896 64472837 ID=MS.gene049808.t1.exon1;Parent=MS.gene049808.t1
chr2.2 CDS 64472837 64472896 64472837 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
chr2.2 exon 64473035 64473165 64473035 ID=MS.gene049808.t1.exon2;Parent=MS.gene049808.t1
chr2.2 CDS 64473035 64473165 64473035 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
chr2.2 exon 64473458 64473526 64473458 ID=MS.gene049808.t1.exon3;Parent=MS.gene049808.t1
chr2.2 CDS 64473458 64473526 64473458 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
chr2.2 exon 64473756 64473827 64473756 ID=MS.gene049808.t1.exon4;Parent=MS.gene049808.t1
chr2.2 CDS 64473756 64473827 64473756 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
chr2.2 exon 64474012 64474084 64474012 ID=MS.gene049808.t1.exon5;Parent=MS.gene049808.t1
chr2.2 CDS 64474012 64474084 64474012 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
chr2.2 exon 64474180 64474236 64474180 ID=MS.gene049808.t1.exon6;Parent=MS.gene049808.t1
chr2.2 CDS 64474180 64474236 64474180 ID=cds.MS.gene049808.t1;Parent=MS.gene049808.t1
Gene Sequence

>MS.gene049808

ATGGAGGTGGGTCAGATGCGGAGACAGTGGGTGGACTACATCAAATCCATGTTCATGGAGGTTAGACCACAAAACACACATTAATTGACTCTTCTGGGATTGTGTTTTTTTTGCTTTTGTTTTTGTTTAGGATGTTTTGTTTTTTCAATTGTTGATTTTGTTGGTCTTTTATTCTTGTTTTGTTTGTGTTGAGAAAAGGGTTTCTTGGATGGCCAGTTTCTGCAACTTCAGCAGCTACAAGATGAGAATAACCCTGAATTTGTTTTTGAAGTTGTTTCTCTTTTCTTTGATGATTCTGAGAGGATTCTCAAAGATCTGTCTTTTGCTGTGTGAGTACTACTACTATCTTTCTGTTCCTTTTTTCATTTCTCTCATGTTGCTTATTAGAGAATATAATGAATTTGGACAAGTGCATGATAAATTAATTGAGCTCCACCTAGAACGAACCGTTCAGGAGAACTATCCTCGGTGGACCAATTCTTGGTTATACTTTACTTACCTCTCGAGTGAACTTCGGATTACCAGGCCCCTTCCCTAGGAACCGGGTCATTAATAGCAAAAAAAATAATAAAAAGAGAGAAGTGCATGATCTTTAATGTTGTTTGGGTTTTACATGTGCAGGGATCAGCAAAGTGTTGACTTCAAAAAAGTTGATGCACATGTTCACCAGTTTAAGGGTAGCAGTGCAAGGTATACAATTTTATTAAAGCCAGCCTAAAATTTATAAATGCTACAGAATCTGAATAAATGGGACTTTTTGTGCGATTACCTGCAATTCTTGTGTAGCTATTAGTTCTTCAATGAGTAGCGTTTTATTTTCCGAATTTCATAACAGACTATTTATGATTTTTCACTTGTTTAAACTATGCTATTATATTATTTTGGTTTGCTGAGATAAGCTCGTATGTTTGATTGACAGCATAGGTGCACAAAGGGTGAAAAATTCCTGCGTTGCTTTTCGCAATTTCTGTGAAGAACAAAACATAGATGCGTAAGTTGTTAATCTTCAGACTAGTTGTTTAATAGTTTTGGTTTCTTGAAGAATTGACAGCTAGTGCAATAGTTTGAAGGACAAGCTACTTGAACAAGACTTAACTGCTTAGATGCTAGAGGAATCAACATGAATTTTTCGCTAATTTCATGGTCTGATCATGTGAAATTTCTAATTTATGCAGGTGCCGCAGGTGTTTGCAGCAAGTGAAGCAAGAGTACTTACTTGTCAAGAATAAGCTTGAAACATTGTTGAGGGTGAGTAAATAGATAAATAACTTGAGCTGAAGAACCTTATATTAAACTTATGGATTTGCAATTTATATGATTTCTTTTATGGATACTTATTGCAGCTTGAGCAGCAGATAGTGGCAGCTGGTGGCTCAATCCCTATGATGGAACTGAACTGA

Protein sequence

>MS.gene049808.t1

MEVGQMRRQWVDYIKSMFMEGFLDGQFLQLQQLQDENNPEFVFEVVSLFFDDSERILKDLSFAVDQQSVDFKKVDAHVHQFKGSSASIGAQRVKNSCVAFRNFCEEQNIDACRRCLQQVKQEYLLVKNKLETLLRLEQQIVAAGGSIPMMELN