Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051577.t1 | XP_003588965.1 | 99.1 | 214 | 2 | 0 | 1 | 214 | 1 | 214 | 1.00E-115 | 426 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051577.t1 | Q84MC2 | 78.9 | 213 | 44 | 1 | 1 | 212 | 1 | 213 | 1.5e-94 | 347.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051577.t1 | G7I9V5 | 99.1 | 214 | 2 | 0 | 1 | 214 | 1 | 214 | 7.4e-116 | 426.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050586 | MS.gene051577 | 0.80965 | 1.70E-50 | -1.69E-46 |
| MS.gene050807 | MS.gene051577 | 0.822773 | 1.98E-53 | -1.69E-46 |
| MS.gene051328 | MS.gene051577 | 0.809228 | 2.10E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene052665 | 0.814033 | 1.89E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene056760 | 0.810882 | 9.24E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene057057 | 0.803271 | 3.77E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene057140 | 0.810835 | 9.46E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene058906 | 0.812752 | 3.62E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene060480 | 0.802911 | 4.48E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene062042 | 0.805258 | 1.46E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene062351 | 0.802267 | 6.09E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene062352 | 0.820187 | 7.83E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene06303 | 0.804838 | 1.78E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene063119 | 0.830468 | 2.89E-55 | -1.69E-46 |
| MS.gene051577 | MS.gene063787 | 0.823347 | 1.45E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene064070 | 0.817128 | 3.87E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene065136 | -0.826007 | 3.43E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene066067 | 0.810639 | 1.04E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene067814 | 0.805101 | 1.57E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene068695 | 0.821469 | 3.97E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene06989 | 0.801492 | 8.78E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene070819 | 0.803621 | 3.19E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene07217 | 0.80956 | 1.78E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene073003 | 0.832759 | 7.87E-56 | -1.69E-46 |
| MS.gene051577 | MS.gene08201 | 0.802627 | 5.13E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene08427 | 0.800148 | 1.65E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene08428 | 0.810069 | 1.38E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene09400 | 0.824309 | 8.66E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene09604 | 0.837754 | 4.31E-57 | -1.69E-46 |
| MS.gene051577 | MS.gene22897 | 0.822726 | 2.03E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene22968 | 0.804196 | 2.43E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene23105 | 0.823427 | 1.39E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene24266 | 0.8015 | 8.75E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene24488 | 0.826945 | 2.05E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene25754 | 0.807323 | 5.35E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene26567 | 0.805812 | 1.11E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene27918 | 0.80811 | 3.64E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene27991 | 0.816178 | 6.32E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene33028 | 0.839475 | 1.55E-57 | -1.69E-46 |
| MS.gene051577 | MS.gene35030 | 0.810924 | 9.05E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene35048 | 0.809864 | 1.53E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene35870 | 0.825514 | 4.50E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene36146 | 0.805214 | 1.49E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene37023 | 0.81571 | 8.04E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene37559 | 0.815924 | 7.20E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene39586 | 0.816525 | 5.29E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene40565 | 0.802933 | 4.43E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene40739 | 0.817332 | 3.48E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene41679 | 0.802731 | 4.88E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene41733 | 0.801985 | 6.95E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene44631 | 0.809776 | 1.60E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene45578 | 0.807443 | 5.04E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene47516 | 0.816794 | 4.60E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene47875 | 0.807707 | 4.43E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene47955 | 0.806493 | 8.01E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene50708 | 0.808022 | 3.80E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene52199 | 0.810714 | 1.00E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene54221 | 0.809201 | 2.13E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene54919 | 0.819078 | 1.40E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene56555 | 0.816805 | 4.58E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene58888 | 0.811228 | 7.78E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene59676 | 0.803285 | 3.75E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene59769 | 0.813123 | 3.00E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene60565 | 0.810455 | 1.14E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene60825 | 0.81079 | 9.67E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene62682 | 0.802001 | 6.90E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene64417 | 0.816995 | 4.15E-52 | -1.69E-46 |
| MS.gene051577 | MS.gene66280 | 0.860382 | 2.15E-63 | -1.69E-46 |
| MS.gene051577 | MS.gene66676 | 0.80701 | 6.23E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene68136 | 0.822598 | 2.17E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene68735 | 0.833198 | 6.12E-56 | -1.69E-46 |
| MS.gene051577 | MS.gene68767 | 0.815012 | 1.15E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene68840 | 0.80053 | 1.38E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene70398 | 0.803526 | 3.34E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene70514 | 0.806323 | 8.70E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene70811 | 0.804105 | 2.53E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene70819 | 0.808984 | 2.37E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene71830 | -0.820689 | 6.01E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene74226 | 0.803517 | 3.36E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene75680 | 0.801195 | 1.01E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene75923 | 0.822312 | 2.53E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene76034 | 0.803813 | 2.91E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene76305 | -0.824656 | 7.17E-54 | -1.69E-46 |
| MS.gene051577 | MS.gene78659 | 0.815039 | 1.13E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene81998 | 0.819942 | 8.91E-53 | -1.69E-46 |
| MS.gene051577 | MS.gene82040 | 0.800883 | 1.17E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene82879 | 0.810739 | 9.92E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene82929 | 0.810972 | 8.83E-51 | -1.69E-46 |
| MS.gene051577 | MS.gene83849 | 0.805494 | 1.30E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene88229 | 0.800483 | 1.41E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene89677 | 0.800778 | 1.23E-48 | -1.69E-46 |
| MS.gene051577 | MS.gene90787 | 0.805496 | 1.30E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene96810 | 0.806881 | 6.63E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene98394 | 0.80272 | 4.91E-49 | -1.69E-46 |
| MS.gene051577 | MS.gene99313 | 0.808846 | 2.53E-50 | -1.69E-46 |
| MS.gene051577 | MS.gene99887 | 0.812402 | 4.32E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene051577 | MS.gene028695 | PPI |
| MS.gene051577 | MS.gene007681 | PPI |
| MS.gene051577 | MS.gene20068 | PPI |
| MS.gene051577 | MS.gene055648 | PPI |
| MS.gene051577 | MS.gene050128 | PPI |
| MS.gene31266 | MS.gene051577 | PPI |
| MS.gene051577 | MS.gene08753 | PPI |
| MS.gene051577 | MS.gene029824 | PPI |
| MS.gene051577 | MS.gene034799 | PPI |
| MS.gene051577 | MS.gene24610 | PPI |
| MS.gene051577 | MS.gene41079 | PPI |
| MS.gene051577 | MS.gene007521 | PPI |
| MS.gene051577 | MS.gene007426 | PPI |
| MS.gene22428 | MS.gene051577 | PPI |
| MS.gene051577 | MS.gene08691 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051577.t1 | MTR_1g015830 | 99.065 | 214 | 2 | 0 | 1 | 214 | 1 | 214 | 1.23e-158 | 437 |
| MS.gene051577.t1 | MTR_1g015830 | 99.383 | 162 | 1 | 0 | 53 | 214 | 1 | 162 | 1.09e-117 | 332 |
| MS.gene051577.t1 | MTR_1g064260 | 66.990 | 206 | 68 | 0 | 7 | 212 | 11 | 216 | 2.98e-103 | 297 |
| MS.gene051577.t1 | MTR_7g101290 | 67.327 | 202 | 66 | 0 | 7 | 208 | 12 | 213 | 7.74e-103 | 296 |
| MS.gene051577.t1 | MTR_0041s0100 | 67.327 | 202 | 66 | 0 | 7 | 208 | 12 | 213 | 7.74e-103 | 296 |
| MS.gene051577.t1 | MTR_2g094790 | 68.687 | 198 | 62 | 0 | 7 | 204 | 11 | 208 | 2.04e-102 | 295 |
| MS.gene051577.t1 | MTR_4g058740 | 69.036 | 197 | 61 | 0 | 8 | 204 | 12 | 208 | 3.34e-102 | 294 |
| MS.gene051577.t1 | MTR_3g113710 | 69.036 | 197 | 61 | 0 | 7 | 203 | 13 | 209 | 1.23e-100 | 291 |
| MS.gene051577.t1 | MTR_1g057020 | 62.441 | 213 | 79 | 1 | 1 | 213 | 1 | 212 | 1.22e-97 | 283 |
| MS.gene051577.t1 | MTR_3g113710 | 70.787 | 178 | 52 | 0 | 7 | 184 | 13 | 190 | 8.54e-92 | 269 |
| MS.gene051577.t1 | MTR_1g064260 | 65.625 | 160 | 55 | 0 | 53 | 212 | 1 | 160 | 1.33e-75 | 225 |
| MS.gene051577.t1 | MTR_3g113710 | 70.896 | 134 | 39 | 0 | 70 | 203 | 10 | 143 | 2.91e-67 | 204 |
| MS.gene051577.t1 | MTR_1g024145 | 67.647 | 102 | 33 | 0 | 25 | 126 | 151 | 252 | 1.62e-43 | 147 |
| MS.gene051577.t1 | MTR_1g024850 | 63.380 | 71 | 26 | 0 | 5 | 75 | 13 | 83 | 1.87e-28 | 103 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene051577.t1 | AT5G11950 | 78.873 | 213 | 44 | 1 | 1 | 212 | 1 | 213 | 6.43e-126 | 354 |
| MS.gene051577.t1 | AT5G11950 | 78.873 | 213 | 44 | 1 | 1 | 212 | 1 | 213 | 6.43e-126 | 354 |
| MS.gene051577.t1 | AT5G11950 | 78.873 | 213 | 44 | 1 | 1 | 212 | 1 | 213 | 6.43e-126 | 354 |
| MS.gene051577.t1 | AT2G28305 | 69.756 | 205 | 62 | 0 | 7 | 211 | 6 | 210 | 2.28e-107 | 307 |
| MS.gene051577.t1 | AT2G37210 | 65.854 | 205 | 69 | 1 | 7 | 211 | 12 | 215 | 1.82e-103 | 298 |
| MS.gene051577.t1 | AT5G06300 | 63.551 | 214 | 77 | 1 | 1 | 213 | 1 | 214 | 5.53e-101 | 291 |
| MS.gene051577.t1 | AT3G53450 | 66.162 | 198 | 67 | 0 | 7 | 204 | 12 | 209 | 5.20e-100 | 289 |
| MS.gene051577.t1 | AT2G37210 | 58.515 | 229 | 70 | 2 | 7 | 211 | 12 | 239 | 2.18e-97 | 283 |
| MS.gene051577.t1 | AT2G28305 | 73.034 | 178 | 48 | 0 | 7 | 184 | 6 | 183 | 6.79e-97 | 280 |
| MS.gene051577.t1 | AT5G11950 | 81.250 | 160 | 30 | 0 | 53 | 212 | 1 | 160 | 1.60e-95 | 276 |
| MS.gene051577.t1 | AT2G35990 | 62.500 | 200 | 75 | 0 | 1 | 200 | 1 | 200 | 7.71e-95 | 276 |
| MS.gene051577.t1 | AT5G03270 | 66.310 | 187 | 63 | 0 | 5 | 191 | 15 | 201 | 3.87e-93 | 271 |
| MS.gene051577.t1 | AT4G35190 | 62.069 | 203 | 77 | 0 | 1 | 203 | 1 | 203 | 6.76e-92 | 269 |
| MS.gene051577.t1 | AT5G03270 | 67.033 | 182 | 60 | 0 | 5 | 186 | 15 | 196 | 4.71e-91 | 266 |
| MS.gene051577.t1 | AT4G35190 | 63.918 | 194 | 68 | 1 | 1 | 194 | 1 | 192 | 2.71e-87 | 256 |
| MS.gene051577.t1 | AT2G28305 | 73.134 | 134 | 36 | 0 | 7 | 140 | 6 | 139 | 4.50e-71 | 213 |
| MS.gene051577.t1 | AT2G35990 | 60.135 | 148 | 59 | 0 | 53 | 200 | 1 | 148 | 2.63e-64 | 196 |
| MS.gene051577.t1 | AT2G35990 | 60.135 | 148 | 59 | 0 | 53 | 200 | 1 | 148 | 2.63e-64 | 196 |
| MS.gene051577.t1 | AT5G26140 | 65.563 | 151 | 30 | 1 | 80 | 208 | 6 | 156 | 8.75e-62 | 190 |
| MS.gene051577.t1 | AT5G26140 | 62.774 | 137 | 29 | 1 | 94 | 208 | 1 | 137 | 2.35e-52 | 166 |
| MS.gene051577.t1 | AT5G26140 | 68.627 | 102 | 32 | 0 | 107 | 208 | 21 | 122 | 7.48e-46 | 149 |
Find 52 sgRNAs with CRISPR-Local
Find 145 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AGGAACATAGATTTGGTTTA+TGG | 0.161614 | 1.3:+6369871 | MS.gene051577:CDS |
| CATCTTCAACATAAGTTCTT+TGG | 0.223030 | 1.3:-6371376 | None:intergenic |
| TGGAACTATGGAAGAGCTTC+TGG | 0.266363 | 1.3:+6371107 | MS.gene051577:CDS |
| TCCTCATAATCAGGAGGATA+TGG | 0.292672 | 1.3:+6371087 | MS.gene051577:intron |
| TCATGTGGCATGAGAGCTTT+TGG | 0.316615 | 1.3:-6370336 | None:intergenic |
| AATGGTGGTTGTCATGTTCT+AGG | 0.326103 | 1.3:+6369940 | MS.gene051577:CDS |
| TGGTGGAAGTGTTGGATTGA+TGG | 0.341974 | 1.3:+6369894 | MS.gene051577:CDS |
| GGTTAAGAGGAACATAGATT+TGG | 0.375088 | 1.3:+6369864 | MS.gene051577:intron |
| GGTGGAAGTGTTGGATTGAT+GGG | 0.382799 | 1.3:+6369895 | MS.gene051577:CDS |
| ACTGAAAACATGGCGGTTGC+CGG | 0.398415 | 1.3:-6369398 | None:intergenic |
| GGTTTGTGTTTCAGGTTAAG+AGG | 0.417871 | 1.3:+6369851 | MS.gene051577:intron |
| AGCTGGCAGATGAAGCAATT+AGG | 0.429412 | 1.3:+6372051 | MS.gene051577:CDS |
| ATCAGGAGGATATGGAACTA+TGG | 0.439372 | 1.3:+6371095 | MS.gene051577:intron |
| ATAGATTTGGTTTATGGTGG+TGG | 0.456028 | 1.3:+6369877 | MS.gene051577:CDS |
| AAGGAAGCAGAAAGTTCAAA+AGG | 0.460142 | 1.3:+6369350 | MS.gene051577:CDS |
| AACATAGATTTGGTTTATGG+TGG | 0.467733 | 1.3:+6369874 | MS.gene051577:CDS |
| GTTTATGGTGGTGGAAGTGT+TGG | 0.468866 | 1.3:+6369886 | MS.gene051577:CDS |
| AGCTTCTGGAGATGATAACT+TGG | 0.479259 | 1.3:+6371121 | MS.gene051577:CDS |
| GTCTTCTGCGGCAGCAACTC+CGG | 0.499932 | 1.3:+6369379 | MS.gene051577:CDS |
| CATGAGGGGCTACATGCTCA+TGG | 0.501960 | 1.3:-6372026 | None:intergenic |
| ATGATAACTTGGGCTCAACT+TGG | 0.508240 | 1.3:+6371132 | MS.gene051577:CDS |
| GACAACTATACTCCGAGCAC+TGG | 0.509009 | 1.3:-6371340 | None:intergenic |
| GTGAAGCAGTAGGTGAAGTA+AGG | 0.514214 | 1.3:+6370803 | MS.gene051577:CDS |
| ATGGTGGTTGTCATGTTCTA+GGG | 0.515119 | 1.3:+6369941 | MS.gene051577:CDS |
| TTCATCTGCCAGCTCTCATG+AGG | 0.517501 | 1.3:-6372042 | None:intergenic |
| AAGTTCATCTGCTAATTGAA+TGG | 0.518634 | 1.3:-6369428 | None:intergenic |
| GTTGGCCTACTGAATGTTGA+TGG | 0.518897 | 1.3:+6371258 | MS.gene051577:CDS |
| TCATCTGCCAGCTCTCATGA+GGG | 0.519557 | 1.3:-6372041 | None:intergenic |
| TTTGACAATGGTGTTGAAGA+AGG | 0.519647 | 1.3:+6371306 | MS.gene051577:CDS |
| CAAAGAACTTATGTTGAAGA+TGG | 0.528044 | 1.3:+6371377 | MS.gene051577:CDS |
| TTGCTTGCATTGTTTGACAA+TGG | 0.530752 | 1.3:+6371294 | MS.gene051577:CDS |
| AGGAAGCAGAAAGTTCAAAA+GGG | 0.552817 | 1.3:+6369351 | MS.gene051577:CDS |
| GGGGCTACATGCTCATGGGC+AGG | 0.554918 | 1.3:-6372021 | None:intergenic |
| AGAACTTATGTTGAAGATGG+AGG | 0.558261 | 1.3:+6371380 | MS.gene051577:CDS |
| ATGTAGCCCCTCATGAGAGC+TGG | 0.566155 | 1.3:+6372034 | MS.gene051577:CDS |
| CAGATATCTGGTGAAGCAGT+AGG | 0.570380 | 1.3:+6370793 | MS.gene051577:intron |
| TGCAATGGCTCAAGAAGCTG+AGG | 0.570924 | 1.3:+6370855 | MS.gene051577:CDS |
| AAGCAATTAGGCAATTATCC+AGG | 0.578634 | 1.3:+6372063 | MS.gene051577:CDS |
| GAGGCATTTATTGCACTCCC+TGG | 0.581953 | 1.3:+6370874 | MS.gene051577:CDS |
| ACAACTATACTCCGAGCACT+GGG | 0.596880 | 1.3:-6371339 | None:intergenic |
| ATGAGGGGCTACATGCTCAT+GGG | 0.601966 | 1.3:-6372025 | None:intergenic |
| TGGCGGCGTCACTGAAAACA+TGG | 0.602579 | 1.3:-6369408 | None:intergenic |
| GCATGAGAGAAAAGCTGCAA+TGG | 0.603274 | 1.3:+6370840 | MS.gene051577:CDS |
| CATCTGCCAGCTCTCATGAG+GGG | 0.620200 | 1.3:-6372040 | None:intergenic |
| AGTAACCATCAACATTCAGT+AGG | 0.622543 | 1.3:-6371263 | None:intergenic |
| GCTTCTGGAGATGATAACTT+GGG | 0.623818 | 1.3:+6371122 | MS.gene051577:CDS |
| AGTAGCACAAATACCTCATG+TGG | 0.625232 | 1.3:-6370351 | None:intergenic |
| GCTTCATTAAGCCCAGTGCT+CGG | 0.625476 | 1.3:+6371328 | MS.gene051577:CDS |
| AAAAGCTCTCATGCCACATG+AGG | 0.636634 | 1.3:+6370338 | MS.gene051577:CDS |
| TTCATCTGCTAATTGAATGG+CGG | 0.661275 | 1.3:-6369425 | None:intergenic |
| AAAAGGGTATGTGTCTTCTG+CGG | 0.690009 | 1.3:+6369367 | MS.gene051577:CDS |
| CGGCGTCACTGAAAACATGG+CGG | 0.784838 | 1.3:-6369405 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TAATAATAATAATAATAATA+AGG | - | chr1.3:6371430-6371449 | None:intergenic | 0.0% |
| !! | ATATCTCAAAAAATGTATAA+TGG | + | chr1.3:6369922-6369941 | MS.gene051577:CDS | 15.0% |
| !! | TAATAATAATAAGGTAAAAG+TGG | - | chr1.3:6371421-6371440 | None:intergenic | 15.0% |
| !!! | ATTGTATTTTTCTTAATCTT+TGG | + | chr1.3:6369492-6369511 | MS.gene051577:intron | 15.0% |
| !!! | TTTTATATAAAAGTGTATCT+GGG | + | chr1.3:6369565-6369584 | MS.gene051577:intron | 15.0% |
| !!! | TTTTTATATAAAAGTGTATC+TGG | + | chr1.3:6369564-6369583 | MS.gene051577:intron | 15.0% |
| !!! | TTTTTATGTGTTTTGTTTTA+TGG | + | chr1.3:6369830-6369849 | MS.gene051577:intron | 15.0% |
| !! | AATAAGCTTAAAACATCTTA+TGG | + | chr1.3:6371675-6371694 | MS.gene051577:intron | 20.0% |
| !! | ATAAGTGTTTATATGCTATA+AGG | - | chr1.3:6370051-6370070 | None:intergenic | 20.0% |
| !! | TCTGATATTGTGTTAATTAA+AGG | + | chr1.3:6371960-6371979 | MS.gene051577:intron | 20.0% |
| !! | TGGATAAACAACTTAATTAA+GGG | + | chr1.3:6371571-6371590 | MS.gene051577:intron | 20.0% |
| !! | TTGGATAAACAACTTAATTA+AGG | + | chr1.3:6371570-6371589 | MS.gene051577:intron | 20.0% |
| !!! | AACTTTTTGAGTTTGAAAAT+TGG | - | chr1.3:6371901-6371920 | None:intergenic | 20.0% |
| !!! | CTTGTAATTTCTTATTTGTA+TGG | - | chr1.3:6370563-6370582 | None:intergenic | 20.0% |
| ! | AAACATTAACATTTCACACA+TGG | - | chr1.3:6369745-6369764 | None:intergenic | 25.0% |
| ! | AATAACACAGATCTTACAAA+GGG | - | chr1.3:6370266-6370285 | None:intergenic | 25.0% |
| ! | ACTTGGAATTCATAAAAAAC+CGG | + | chr1.3:6371149-6371168 | MS.gene051577:CDS | 25.0% |
| ! | AGAGTAATGTAAATTACTGT+TGG | - | chr1.3:6371943-6371962 | None:intergenic | 25.0% |
| ! | GAAACTATCATCTTAGTAAT+TGG | + | chr1.3:6370760-6370779 | MS.gene051577:intron | 25.0% |
| ! | TCTCAAAAAATGTATAATGG+TGG | + | chr1.3:6369925-6369944 | MS.gene051577:CDS | 25.0% |
| ! | TGAACTAACTACTATCATAA+AGG | - | chr1.3:6370703-6370722 | None:intergenic | 25.0% |
| ! | TTATGACATTTGACAATCAA+AGG | + | chr1.3:6370505-6370524 | MS.gene051577:intron | 25.0% |
| !! | ATTAAGTTGTTTATCCAAAC+AGG | - | chr1.3:6371568-6371587 | None:intergenic | 25.0% |
| !! | ATTTTCATGAGACTTGATAT+AGG | - | chr1.3:6371764-6371783 | None:intergenic | 25.0% |
| !! | TGTAGTGTGATAATGTTAAT+AGG | + | chr1.3:6371828-6371847 | MS.gene051577:intron | 25.0% |
| !! | TTAAGTTGTTTATCCAAACA+GGG | - | chr1.3:6371567-6371586 | None:intergenic | 25.0% |
| !! | TTTGATAATAGTTTATCGTC+TGG | + | chr1.3:6370472-6370491 | MS.gene051577:intron | 25.0% |
| !! | TTTTCATGAGACTTGATATA+GGG | - | chr1.3:6371763-6371782 | None:intergenic | 25.0% |
| !!! | TTCTTTTATTTTTCGTGCAT+TGG | + | chr1.3:6371005-6371024 | MS.gene051577:intron | 25.0% |
| AAATTACAAGAATGTTAGCG+AGG | + | chr1.3:6370573-6370592 | MS.gene051577:intron | 30.0% | |
| AAGTAACATCATAACGTTAC+CGG | - | chr1.3:6371171-6371190 | None:intergenic | 30.0% | |
| AAGTTCATCTGCTAATTGAA+TGG | - | chr1.3:6369431-6369450 | None:intergenic | 30.0% | |
| AATAAGTTGTTTATCCGAAC+AGG | - | chr1.3:6370023-6370042 | None:intergenic | 30.0% | |
| AATGTAGAGTAGTACATATC+AGG | - | chr1.3:6370304-6370323 | None:intergenic | 30.0% | |
| AATTACAAGAATGTTAGCGA+GGG | + | chr1.3:6370574-6370593 | MS.gene051577:intron | 30.0% | |
| ACTTTGTTGGATAAAATGCT+TGG | + | chr1.3:6371187-6371206 | MS.gene051577:intron | 30.0% | |
| ATAAGTTGTTTATCCGAACA+GGG | - | chr1.3:6370022-6370041 | None:intergenic | 30.0% | |
| ATGTACTACTCTACATTTAC+AGG | + | chr1.3:6370307-6370326 | MS.gene051577:intron | 30.0% | |
| CAAAGAACTTATGTTGAAGA+TGG | + | chr1.3:6371377-6371396 | MS.gene051577:CDS | 30.0% | |
| CAATAACACAGATCTTACAA+AGG | - | chr1.3:6370267-6370286 | None:intergenic | 30.0% | |
| CATCTTCAACATAAGTTCTT+TGG | - | chr1.3:6371379-6371398 | None:intergenic | 30.0% | |
| GAGTTTATTTGAAGAGCTTA+TGG | - | chr1.3:6371719-6371738 | None:intergenic | 30.0% | |
| GGGTCAACATATGATTATAA+CGG | - | chr1.3:6371219-6371238 | None:intergenic | 30.0% | |
| TAACACTATAACAGTTACAC+AGG | - | chr1.3:6370381-6370400 | None:intergenic | 30.0% | |
| TAGTAGTTAGTTCATTGTCA+TGG | + | chr1.3:6370709-6370728 | MS.gene051577:intron | 30.0% | |
| TGAAAATAACTTGACAGACA+TGG | - | chr1.3:6371609-6371628 | None:intergenic | 30.0% | |
| TGTACTACTCTACATTTACA+GGG | + | chr1.3:6370308-6370327 | MS.gene051577:intron | 30.0% | |
| TTGATCTCTAGCAATGAATT+GGG | + | chr1.3:6370444-6370463 | MS.gene051577:intron | 30.0% | |
| TTTGATCTCTAGCAATGAAT+TGG | + | chr1.3:6370443-6370462 | MS.gene051577:intron | 30.0% | |
| ! | AACATAGATTTGGTTTATGG+TGG | + | chr1.3:6369874-6369893 | MS.gene051577:CDS | 30.0% |
| ! | AACTCTTTGAAGAGTTATTC+AGG | - | chr1.3:6370905-6370924 | None:intergenic | 30.0% |
| ! | AGGAACATAGATTTGGTTTA+TGG | + | chr1.3:6369871-6369890 | MS.gene051577:CDS | 30.0% |
| ! | ATATAAAAGTGTATCTGGGT+AGG | + | chr1.3:6369569-6369588 | MS.gene051577:intron | 30.0% |
| ! | CTTCTGATAATGACCTATTT+GGG | + | chr1.3:6370661-6370680 | MS.gene051577:intron | 30.0% |
| ! | GTGTACAGAAACATTGATTA+AGG | + | chr1.3:6369792-6369811 | MS.gene051577:intron | 30.0% |
| ! | GTTGTTGATTGATATCACTT+TGG | + | chr1.3:6369996-6370015 | MS.gene051577:intron | 30.0% |
| ! | TCTTCTGATAATGACCTATT+TGG | + | chr1.3:6370660-6370679 | MS.gene051577:intron | 30.0% |
| ! | TGTTTTCATATAAGCTATCC+TGG | + | chr1.3:6371640-6371659 | MS.gene051577:intron | 30.0% |
| !! | ACGTTATGATGTTACTTTGT+TGG | + | chr1.3:6371174-6371193 | MS.gene051577:intron | 30.0% |
| !! | ATAGGAGTTTACTGTTTTTC+TGG | + | chr1.3:6371846-6371865 | MS.gene051577:intron | 30.0% |
| !! | CTTATTTTCACAAATTCTCC+AGG | - | chr1.3:6371661-6371680 | None:intergenic | 30.0% |
| !! | TGTTTTATGGTTTGTGTTTC+AGG | + | chr1.3:6369843-6369862 | MS.gene051577:intron | 30.0% |
| !!! | CTATTGATTTTCGTTCTACA+CGG | + | chr1.3:6370414-6370433 | MS.gene051577:intron | 30.0% |
| !!! | GGTTTATTTTGACAGATATC+TGG | + | chr1.3:6370781-6370800 | MS.gene051577:intron | 30.0% |
| AAGCAATTAGGCAATTATCC+AGG | + | chr1.3:6372063-6372082 | MS.gene051577:CDS | 35.0% | |
| AAGGAAGCAGAAAGTTCAAA+AGG | + | chr1.3:6369350-6369369 | MS.gene051577:CDS | 35.0% | |
| AGAACTTATGTTGAAGATGG+AGG | + | chr1.3:6371380-6371399 | MS.gene051577:CDS | 35.0% | |
| AGAGTTATTCAGGTAATACC+AGG | - | chr1.3:6370895-6370914 | None:intergenic | 35.0% | |
| AGGAAGCAGAAAGTTCAAAA+GGG | + | chr1.3:6369351-6369370 | MS.gene051577:CDS | 35.0% | |
| AGTAACCATCAACATTCAGT+AGG | - | chr1.3:6371266-6371285 | None:intergenic | 35.0% | |
| ATTCTATCCTCATAATCAGG+AGG | + | chr1.3:6371081-6371100 | MS.gene051577:intron | 35.0% | |
| CAAAGTTCCTTATATATCGC+AGG | - | chr1.3:6371810-6371829 | None:intergenic | 35.0% | |
| GAGTCTAAATTGATGAATGG+TGG | + | chr1.3:6371496-6371515 | MS.gene051577:intron | 35.0% | |
| GAGTTATTCAGGTAATACCA+GGG | - | chr1.3:6370894-6370913 | None:intergenic | 35.0% | |
| GATTATGAGGATAGAATGGA+GGG | - | chr1.3:6371078-6371097 | None:intergenic | 35.0% | |
| GCTGAGTCTAAATTGATGAA+TGG | + | chr1.3:6371493-6371512 | MS.gene051577:intron | 35.0% | |
| GGTTAAGAGGAACATAGATT+TGG | + | chr1.3:6369864-6369883 | MS.gene051577:intron | 35.0% | |
| GTATGTTAGGGTAATGTCAA+TGG | + | chr1.3:6370602-6370621 | MS.gene051577:intron | 35.0% | |
| TACAAAGGGTCTGTTTGAAT+AGG | - | chr1.3:6370252-6370271 | None:intergenic | 35.0% | |
| TCAAATAAGCCAATCACAAC+AGG | + | chr1.3:6371737-6371756 | MS.gene051577:intron | 35.0% | |
| TCCATTCTATCCTCATAATC+AGG | + | chr1.3:6371078-6371097 | MS.gene051577:intron | 35.0% | |
| TCCTGATTATGAGGATAGAA+TGG | - | chr1.3:6371082-6371101 | None:intergenic | 35.0% | |
| TGATTATGAGGATAGAATGG+AGG | - | chr1.3:6371079-6371098 | None:intergenic | 35.0% | |
| TTCATCTGCTAATTGAATGG+CGG | - | chr1.3:6369428-6369447 | None:intergenic | 35.0% | |
| TTGCTTGCATTGTTTGACAA+TGG | + | chr1.3:6371294-6371313 | MS.gene051577:CDS | 35.0% | |
| TTTACCTCCTGCGATATATA+AGG | + | chr1.3:6371800-6371819 | MS.gene051577:intron | 35.0% | |
| ! | AATGGAGGGTATGAGAAAAA+AGG | - | chr1.3:6371064-6371083 | None:intergenic | 35.0% |
| ! | ATAGATTTGGTTTATGGTGG+TGG | + | chr1.3:6369877-6369896 | MS.gene051577:CDS | 35.0% |
| ! | ATTGATGACAACATCAACAC+AGG | - | chr1.3:6369687-6369706 | None:intergenic | 35.0% |
| ! | GAGCAAATTTTCTCCCAAAT+AGG | - | chr1.3:6370677-6370696 | None:intergenic | 35.0% |
| ! | TTGATGACAACATCAACACA+GGG | - | chr1.3:6369686-6369705 | None:intergenic | 35.0% |
| ! | TTTGACAATGGTGTTGAAGA+AGG | + | chr1.3:6371306-6371325 | MS.gene051577:CDS | 35.0% |
| AAATATCATCCTGCACAGGT+TGG | + | chr1.3:6371240-6371259 | MS.gene051577:intron | 40.0% | |
| AATGGTGGTTGTCATGTTCT+AGG | + | chr1.3:6369940-6369959 | MS.gene051577:CDS | 40.0% | |
| AGCGAGGGTTTATGTATGTT+AGG | + | chr1.3:6370589-6370608 | MS.gene051577:intron | 40.0% | |
| AGTAGCACAAATACCTCATG+TGG | - | chr1.3:6370354-6370373 | None:intergenic | 40.0% | |
| AGTTCCTTATATATCGCAGG+AGG | - | chr1.3:6371807-6371826 | None:intergenic | 40.0% | |
| ATCAGGAGGATATGGAACTA+TGG | + | chr1.3:6371095-6371114 | MS.gene051577:intron | 40.0% | |
| ATGATAACTTGGGCTCAACT+TGG | + | chr1.3:6371132-6371151 | MS.gene051577:CDS | 40.0% | |
| ATGGTGGTTGTCATGTTCTA+GGG | + | chr1.3:6369941-6369960 | MS.gene051577:CDS | 40.0% | |
| GCGAGGGTTTATGTATGTTA+GGG | + | chr1.3:6370590-6370609 | MS.gene051577:intron | 40.0% | |
| TAGGGTAATGTCAATGGAGT+GGG | + | chr1.3:6370608-6370627 | MS.gene051577:intron | 40.0% | |
| TCCATATCCTCCTGATTATG+AGG | - | chr1.3:6371091-6371110 | None:intergenic | 40.0% | |
| TCCTCATAATCAGGAGGATA+TGG | + | chr1.3:6371087-6371106 | MS.gene051577:intron | 40.0% | |
| TGAGAGTCTATAACCCTGTT+TGG | + | chr1.3:6371551-6371570 | MS.gene051577:intron | 40.0% | |
| TTAGGGTAATGTCAATGGAG+TGG | + | chr1.3:6370607-6370626 | MS.gene051577:intron | 40.0% | |
| ! | AAAAGGGTATGTGTCTTCTG+CGG | + | chr1.3:6369367-6369386 | MS.gene051577:CDS | 40.0% |
| ! | ACCTGTGCAGGATGATATTT+TGG | - | chr1.3:6371240-6371259 | None:intergenic | 40.0% |
| ! | AGCTTCTGGAGATGATAACT+TGG | + | chr1.3:6371121-6371140 | MS.gene051577:CDS | 40.0% |
| ! | CATTCTGCATTTTCTTGTCC+TGG | - | chr1.3:6372084-6372103 | None:intergenic | 40.0% |
| ! | CCTGTGCAGGATGATATTTT+GGG | - | chr1.3:6371239-6371258 | None:intergenic | 40.0% |
| ! | GCTTCTGGAGATGATAACTT+GGG | + | chr1.3:6371122-6371141 | MS.gene051577:CDS | 40.0% |
| !! | GGTTTGTGTTTCAGGTTAAG+AGG | + | chr1.3:6369851-6369870 | MS.gene051577:intron | 40.0% |
| AAAAGCTCTCATGCCACATG+AGG | + | chr1.3:6370338-6370357 | MS.gene051577:CDS | 45.0% | |
| ACAACTATACTCCGAGCACT+GGG | - | chr1.3:6371342-6371361 | None:intergenic | 45.0% | |
| AGCTGGCAGATGAAGCAATT+AGG | + | chr1.3:6372051-6372070 | MS.gene051577:CDS | 45.0% | |
| AGGGTAATGTCAATGGAGTG+GGG | + | chr1.3:6370609-6370628 | MS.gene051577:intron | 45.0% | |
| CAGATATCTGGTGAAGCAGT+AGG | + | chr1.3:6370793-6370812 | MS.gene051577:intron | 45.0% | |
| CCCAAAATATCATCCTGCAC+AGG | + | chr1.3:6371236-6371255 | MS.gene051577:intron | 45.0% | |
| GATATAGGGCCTGTTGTGAT+TGG | - | chr1.3:6371749-6371768 | None:intergenic | 45.0% | |
| GATATCACTTTGGCCCTGTT+CGG | + | chr1.3:6370006-6370025 | MS.gene051577:intron | 45.0% | |
| GCATGAGAGAAAAGCTGCAA+TGG | + | chr1.3:6370840-6370859 | MS.gene051577:CDS | 45.0% | |
| GTGAAGCAGTAGGTGAAGTA+AGG | + | chr1.3:6370803-6370822 | MS.gene051577:CDS | 45.0% | |
| TGGAACTATGGAAGAGCTTC+TGG | + | chr1.3:6371107-6371126 | MS.gene051577:CDS | 45.0% | |
| ! | GTTGGCCTACTGAATGTTGA+TGG | + | chr1.3:6371258-6371277 | MS.gene051577:CDS | 45.0% |
| ! | TCATGTGGCATGAGAGCTTT+TGG | - | chr1.3:6370339-6370358 | None:intergenic | 45.0% |
| !! | GGTGGAAGTGTTGGATTGAT+GGG | + | chr1.3:6369895-6369914 | MS.gene051577:CDS | 45.0% |
| !! | GTTTATGGTGGTGGAAGTGT+TGG | + | chr1.3:6369886-6369905 | MS.gene051577:CDS | 45.0% |
| !! | TGGTGGAAGTGTTGGATTGA+TGG | + | chr1.3:6369894-6369913 | MS.gene051577:CDS | 45.0% |
| ACTGAAAACATGGCGGTTGC+CGG | - | chr1.3:6369401-6369420 | None:intergenic | 50.0% | |
| ATGAGGGGCTACATGCTCAT+GGG | - | chr1.3:6372028-6372047 | None:intergenic | 50.0% | |
| GACAACTATACTCCGAGCAC+TGG | - | chr1.3:6371343-6371362 | None:intergenic | 50.0% | |
| GCTTCATTAAGCCCAGTGCT+CGG | + | chr1.3:6371328-6371347 | MS.gene051577:CDS | 50.0% | |
| TCATCTGCCAGCTCTCATGA+GGG | - | chr1.3:6372044-6372063 | None:intergenic | 50.0% | |
| TGCAATGGCTCAAGAAGCTG+AGG | + | chr1.3:6370855-6370874 | MS.gene051577:CDS | 50.0% | |
| TTCATCTGCCAGCTCTCATG+AGG | - | chr1.3:6372045-6372064 | None:intergenic | 50.0% | |
| ! | GAGGCATTTATTGCACTCCC+TGG | + | chr1.3:6370874-6370893 | MS.gene051577:CDS | 50.0% |
| ATGTAGCCCCTCATGAGAGC+TGG | + | chr1.3:6372034-6372053 | MS.gene051577:CDS | 55.0% | |
| CATCTGCCAGCTCTCATGAG+GGG | - | chr1.3:6372043-6372062 | None:intergenic | 55.0% | |
| CATGAGGGGCTACATGCTCA+TGG | - | chr1.3:6372029-6372048 | None:intergenic | 55.0% | |
| CGGCGTCACTGAAAACATGG+CGG | - | chr1.3:6369408-6369427 | None:intergenic | 55.0% | |
| TGGCGGCGTCACTGAAAACA+TGG | - | chr1.3:6369411-6369430 | None:intergenic | 55.0% | |
| TTCAGTAGGCCAACCTGTGC+AGG | - | chr1.3:6371252-6371271 | None:intergenic | 55.0% | |
| GTCTTCTGCGGCAGCAACTC+CGG | + | chr1.3:6369379-6369398 | MS.gene051577:CDS | 60.0% | |
| GGGGCTACATGCTCATGGGC+AGG | - | chr1.3:6372024-6372043 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.3 | gene | 6369343 | 6372104 | 6369343 | ID=MS.gene051577 |
| chr1.3 | mRNA | 6369343 | 6372104 | 6369343 | ID=MS.gene051577.t1;Parent=MS.gene051577 |
| chr1.3 | exon | 6369343 | 6369450 | 6369343 | ID=MS.gene051577.t1.exon1;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6369343 | 6369450 | 6369343 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6369865 | 6369962 | 6369865 | ID=MS.gene051577.t1.exon2;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6369865 | 6369962 | 6369865 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6370329 | 6370359 | 6370329 | ID=MS.gene051577.t1.exon3;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6370329 | 6370359 | 6370329 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6370796 | 6370895 | 6370796 | ID=MS.gene051577.t1.exon4;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6370796 | 6370895 | 6370796 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6371100 | 6371170 | 6371100 | ID=MS.gene051577.t1.exon5;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6371100 | 6371170 | 6371100 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6371258 | 6371401 | 6371258 | ID=MS.gene051577.t1.exon6;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6371258 | 6371401 | 6371258 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| chr1.3 | exon | 6372012 | 6372104 | 6372012 | ID=MS.gene051577.t1.exon7;Parent=MS.gene051577.t1 |
| chr1.3 | CDS | 6372012 | 6372104 | 6372012 | ID=cds.MS.gene051577.t1;Parent=MS.gene051577.t1 |
| Gene Sequence |
| Protein sequence |
