Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049254 | MS.gene52518 | 0.803629 | 3.18E-49 | -1.69E-46 |
| MS.gene051594 | MS.gene52518 | 0.805776 | 1.13E-49 | -1.69E-46 |
| MS.gene055818 | MS.gene52518 | 0.825371 | 4.86E-54 | -1.69E-46 |
| MS.gene056174 | MS.gene52518 | 0.806705 | 7.23E-50 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 23 sgRNAs with CRISPR-Local
Find 123 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCCAAATCTTCTCATTT+TGG | 0.143116 | 7.2:+62695307 | None:intergenic |
| AACTTTGTCTCTAGGCTTGC+AGG | 0.274208 | 7.2:-62696864 | MS.gene52518:intron |
| AGCGCCAAAATGAGAAGATT+TGG | 0.293225 | 7.2:-62695311 | MS.gene52518:CDS |
| TTCTGTGGCTGCAGTGGCTG+TGG | 0.414645 | 7.2:-62695611 | MS.gene52518:intron |
| GCGCCAAAATGAGAAGATTT+GGG | 0.435664 | 7.2:-62695310 | MS.gene52518:CDS |
| TAAGTGAGGCGGCAGGGCCG+TGG | 0.445627 | 7.2:-62695454 | MS.gene52518:CDS |
| TCACGCAATGGAAGGAGATT+AGG | 0.445908 | 7.2:-62695238 | MS.gene52518:CDS |
| GCAGATGGAACTTTGTCTCT+AGG | 0.487647 | 7.2:-62696872 | MS.gene52518:CDS |
| AGCAGACTGTAGCAGCGAAT+AGG | 0.488975 | 7.2:-62695214 | MS.gene52518:CDS |
| AAGAAGGATGTTGAATGGAA+TGG | 0.494548 | 7.2:-62694821 | MS.gene52518:CDS |
| TTATAAAGAAGGATGTTGAA+TGG | 0.501223 | 7.2:-62694826 | MS.gene52518:CDS |
| AGCAGCATGGTGAAGGCAGA+TGG | 0.518000 | 7.2:-62696887 | None:intergenic |
| TAGATCTGAGATTATAAAGA+AGG | 0.519444 | 7.2:-62694837 | MS.gene52518:CDS |
| TTGTCCACAGCTTCAAGCAA+AGG | 0.523678 | 7.2:+62695573 | None:intergenic |
| AAAACCTTTGCTTGAAGCTG+TGG | 0.526822 | 7.2:-62695577 | MS.gene52518:CDS |
| AAGTGAGGCGGCAGGGCCGT+GGG | 0.549357 | 7.2:-62695453 | MS.gene52518:CDS |
| TATGTCAAGCAGCATGGTGA+AGG | 0.550639 | 7.2:-62696894 | None:intergenic |
| TGAGAAGATTTGGGAAGACA+AGG | 0.554945 | 7.2:-62695301 | MS.gene52518:CDS |
| GCTGTGGACAAGATGAAAGA+AGG | 0.584388 | 7.2:-62695561 | MS.gene52518:intron |
| CGAGACTATCACGCAATGGA+AGG | 0.589824 | 7.2:-62695246 | MS.gene52518:CDS |
| ATAATCTCAGATCTAGTGCA+CGG | 0.625982 | 7.2:+62694845 | None:intergenic |
| GAAACATGCAAATTCTCCCA+CGG | 0.647808 | 7.2:+62695437 | None:intergenic |
| TGCACGAGACTATCACGCAA+TGG | 0.649469 | 7.2:-62695250 | MS.gene52518:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TTAATCATATATAAGTATTA+CGG | - | chr7.2:62695982-62696001 | MS.gene52518:intron | 10.0% |
| !!! | TTTTAATGTTATTACTAATA+TGG | - | chr7.2:62696316-62696335 | MS.gene52518:intron | 10.0% |
| !! | AATGAGTTCAATATTAAAAT+TGG | - | chr7.2:62695004-62695023 | MS.gene52518:intron | 15.0% |
| !! | CAATACATCAAATTAAAAAA+AGG | + | chr7.2:62695948-62695967 | None:intergenic | 15.0% |
| !! | TAATCATATATAAGTATTAC+GGG | - | chr7.2:62695983-62696002 | MS.gene52518:intron | 15.0% |
| !!! | AAATTAAGCTTTTAAATTTG+TGG | - | chr7.2:62695051-62695070 | MS.gene52518:intron | 15.0% |
| !! | ACAAAAATGTGTATAAAGTT+TGG | + | chr7.2:62694867-62694886 | None:intergenic | 20.0% |
| !! | ATCAAAAGTAAAGAAATTAC+AGG | + | chr7.2:62695387-62695406 | None:intergenic | 20.0% |
| !! | TGAAAATATATTACTCATCA+AGG | - | chr7.2:62695187-62695206 | MS.gene52518:intron | 20.0% |
| !! | TGTACTGTATTTATTTGTAA+GGG | - | chr7.2:62695878-62695897 | MS.gene52518:intron | 20.0% |
| !! | TTAGTTTATGTAGAGTAAAA+AGG | + | chr7.2:62695267-62695286 | None:intergenic | 20.0% |
| !! | TTTCTTCTTTATCTCTTAAA+AGG | - | chr7.2:62696731-62696750 | MS.gene52518:intron | 20.0% |
| !!! | ATTTATGGATTAACTAACTT+TGG | + | chr7.2:62694919-62694938 | None:intergenic | 20.0% |
| !!! | TGGTTATATTTTTTTTTCTG+TGG | - | chr7.2:62696072-62696091 | MS.gene52518:intron | 20.0% |
| ! | AATCAGAATTCATACCAAAA+AGG | + | chr7.2:62696050-62696069 | None:intergenic | 25.0% |
| ! | AATTTAGAAAACTGAAGGTT+AGG | + | chr7.2:62695036-62695055 | None:intergenic | 25.0% |
| ! | AGCTTAATTTAGAAAACTGA+AGG | + | chr7.2:62695041-62695060 | None:intergenic | 25.0% |
| ! | AGTCAAAATTCACTGAAAAT+TGG | - | chr7.2:62694950-62694969 | MS.gene52518:intron | 25.0% |
| ! | ATTTAGAAAACTGAAGGTTA+GGG | + | chr7.2:62695035-62695054 | None:intergenic | 25.0% |
| ! | CAAAGTCCAAATCTTATATA+TGG | + | chr7.2:62695517-62695536 | None:intergenic | 25.0% |
| ! | CATATATAAGATTTGGACTT+TGG | - | chr7.2:62695515-62695534 | MS.gene52518:intron | 25.0% |
| ! | CTGTACTGTATTTATTTGTA+AGG | - | chr7.2:62695877-62695896 | MS.gene52518:intron | 25.0% |
| ! | CTTTATATACAAAAAAGTGG+AGG | - | chr7.2:62695431-62695450 | MS.gene52518:CDS | 25.0% |
| ! | CTTTATCTCTTAAAAGGAAA+TGG | - | chr7.2:62696737-62696756 | MS.gene52518:intron | 25.0% |
| ! | GACCTTTATATACAAAAAAG+TGG | - | chr7.2:62695428-62695447 | MS.gene52518:CDS | 25.0% |
| ! | GACTTGAATAACTCAATTTA+TGG | + | chr7.2:62694934-62694953 | None:intergenic | 25.0% |
| ! | TGTTAATCTATGTAGTTGTA+GGG | - | chr7.2:62695702-62695721 | MS.gene52518:intron | 25.0% |
| ! | TTAGCAAAATGCCTTAAAAA+TGG | + | chr7.2:62695760-62695779 | None:intergenic | 25.0% |
| ! | TTATAAAGAAGGATGTTGAA+TGG | - | chr7.2:62696872-62696891 | MS.gene52518:CDS | 25.0% |
| !! | AATATATTTTCATCTCCATG+AGG | + | chr7.2:62695179-62695198 | None:intergenic | 25.0% |
| !! | GTGAATAAACTATTCCTTTT+TGG | - | chr7.2:62696033-62696052 | MS.gene52518:intron | 25.0% |
| !! | TAGATCTGAGATTATAAAGA+AGG | - | chr7.2:62696861-62696880 | MS.gene52518:intron | 25.0% |
| !! | TTAAGCTTTTAAATTTGTGG+AGG | - | chr7.2:62695054-62695073 | MS.gene52518:intron | 25.0% |
| !!! | CTCCACTTTTTTGTATATAA+AGG | + | chr7.2:62695433-62695452 | None:intergenic | 25.0% |
| !!! | TCTTTACTTTTGATACACAA+TGG | - | chr7.2:62695393-62695412 | MS.gene52518:intron | 25.0% |
| AACAATCGACATTTGTTTGT+GGG | - | chr7.2:62696199-62696218 | MS.gene52518:intron | 30.0% | |
| AATATTGAACTCATTCCATC+AGG | + | chr7.2:62694999-62695018 | None:intergenic | 30.0% | |
| ACCACACTTAAATATTTGCT+AGG | + | chr7.2:62695818-62695837 | None:intergenic | 30.0% | |
| ATTCACAAACCCTTACAAAA+GGG | + | chr7.2:62696019-62696038 | None:intergenic | 30.0% | |
| CAGAAACTATAAAAGAACCA+AGG | - | chr7.2:62695558-62695577 | MS.gene52518:intron | 30.0% | |
| CATTCATAATCCTTATCATG+CGG | - | chr7.2:62695222-62695241 | MS.gene52518:CDS | 30.0% | |
| CTTATGAATCAAAGAGAAAC+TGG | + | chr7.2:62695350-62695369 | None:intergenic | 30.0% | |
| CTTTGATGGAATGCAAATAT+TGG | + | chr7.2:62696812-62696831 | None:intergenic | 30.0% | |
| GCTCTATTATACAACTTTGA+TGG | + | chr7.2:62696826-62696845 | None:intergenic | 30.0% | |
| GTCCAAATCTTATATATGGA+AGG | + | chr7.2:62695513-62695532 | None:intergenic | 30.0% | |
| GTGTTAATCTATGTAGTTGT+AGG | - | chr7.2:62695701-62695720 | MS.gene52518:intron | 30.0% | |
| GTTCATCAAGTTTCAGTATA+TGG | + | chr7.2:62695295-62695314 | None:intergenic | 30.0% | |
| TAGAGTAAAAAGGCAATTCT+TGG | + | chr7.2:62695257-62695276 | None:intergenic | 30.0% | |
| TATAAAAGAACCAAGGTCAT+TGG | - | chr7.2:62695565-62695584 | MS.gene52518:CDS | 30.0% | |
| TATTCACAAACCCTTACAAA+AGG | + | chr7.2:62696020-62696039 | None:intergenic | 30.0% | |
| TTAAATTCACCTACCATTAG+TGG | + | chr7.2:62696596-62696615 | None:intergenic | 30.0% | |
| ! | AAGAGAAACTGGTTTAATAG+AGG | + | chr7.2:62695339-62695358 | None:intergenic | 30.0% |
| ! | AATTTTACAGTTCAGCAGTA+CGG | - | chr7.2:62696564-62696583 | MS.gene52518:intron | 30.0% |
| ! | TGGATACAAAGTACTATGTT+AGG | + | chr7.2:62694899-62694918 | None:intergenic | 30.0% |
| ! | TTGGTATGAATTCTGATTTG+TGG | - | chr7.2:62696052-62696071 | MS.gene52518:intron | 30.0% |
| !! | TAAGATTTGGACTTTGGTTT+TGG | - | chr7.2:62695521-62695540 | MS.gene52518:intron | 30.0% |
| !!! | AGTGTGGTTAGATTCTTTTT+TGG | - | chr7.2:62695830-62695849 | MS.gene52518:intron | 30.0% |
| !!! | AGTTTTAAACACACTAGACT+AGG | - | chr7.2:62695138-62695157 | MS.gene52518:intron | 30.0% |
| AAAAGTTGCATGGTAGGAAT+TGG | - | chr7.2:62695725-62695744 | MS.gene52518:intron | 35.0% | |
| AAAGTTGCATGGTAGGAATT+GGG | - | chr7.2:62695726-62695745 | MS.gene52518:intron | 35.0% | |
| AAGAAGGATGTTGAATGGAA+TGG | - | chr7.2:62696877-62696896 | MS.gene52518:CDS | 35.0% | |
| AGGCGAAAAAAAAGTAAGTG+AGG | - | chr7.2:62696230-62696249 | MS.gene52518:intron | 35.0% | |
| ATAATCTCAGATCTAGTGCA+CGG | + | chr7.2:62696856-62696875 | None:intergenic | 35.0% | |
| ATTGCAATCAATGTTCCTCA+TGG | - | chr7.2:62695161-62695180 | MS.gene52518:intron | 35.0% | |
| CGAAAAAAAAGTAAGTGAGG+CGG | - | chr7.2:62696233-62696252 | MS.gene52518:intron | 35.0% | |
| GAACAATCGACATTTGTTTG+TGG | - | chr7.2:62696198-62696217 | MS.gene52518:intron | 35.0% | |
| GAGAAAAATTCCGCATGATA+AGG | + | chr7.2:62695235-62695254 | None:intergenic | 35.0% | |
| GCCTAGCAAATATTTAAGTG+TGG | - | chr7.2:62695814-62695833 | MS.gene52518:intron | 35.0% | |
| GGCCTTCCATATATAAGATT+TGG | - | chr7.2:62695508-62695527 | MS.gene52518:intron | 35.0% | |
| TGCAGATAACTGGAATTGAA+AGG | - | chr7.2:62695487-62695506 | MS.gene52518:intron | 35.0% | |
| TTTGTTTGTGGGATGTGATA+AGG | - | chr7.2:62696210-62696229 | MS.gene52518:intron | 35.0% | |
| ! | CTTCCCAAATCTTCTCATTT+TGG | + | chr7.2:62696394-62696413 | None:intergenic | 35.0% |
| ! | TACTAATATGGAGCACCATA+AGG | - | chr7.2:62696328-62696347 | MS.gene52518:intron | 35.0% |
| ! | TAGCAAAGTCAATAGCCTTA+TGG | + | chr7.2:62696346-62696365 | None:intergenic | 35.0% |
| ! | TGACTTTGCTATGATGATGT+GGG | - | chr7.2:62696355-62696374 | MS.gene52518:intron | 35.0% |
| ! | TTGACTTTGCTATGATGATG+TGG | - | chr7.2:62696354-62696373 | MS.gene52518:intron | 35.0% |
| !! | GGGAGTTTGTTCCATTTTTA+AGG | - | chr7.2:62695746-62695765 | MS.gene52518:intron | 35.0% |
| !!! | TGTTGTTTTCTCTTTGCTTG+TGG | - | chr7.2:62695607-62695626 | MS.gene52518:intron | 35.0% |
| AAAACCTTTGCTTGAAGCTG+TGG | - | chr7.2:62696121-62696140 | MS.gene52518:intron | 40.0% | |
| AGCGCCAAAATGAGAAGATT+TGG | - | chr7.2:62696387-62696406 | MS.gene52518:intron | 40.0% | |
| AGTTGTAGGGAAAAGTTGCA+TGG | - | chr7.2:62695715-62695734 | MS.gene52518:intron | 40.0% | |
| CAAAGAGAAAACAACAGACC+AGG | + | chr7.2:62695603-62695622 | None:intergenic | 40.0% | |
| GAAACATGCAAATTCTCCCA+CGG | + | chr7.2:62696264-62696283 | None:intergenic | 40.0% | |
| GAACTTGATCTGCAGATAAC+TGG | - | chr7.2:62695477-62695496 | MS.gene52518:intron | 40.0% | |
| GCGCCAAAATGAGAAGATTT+GGG | - | chr7.2:62696388-62696407 | MS.gene52518:intron | 40.0% | |
| TATGTGTATCGGAACTCTAG+AGG | - | chr7.2:62696667-62696686 | MS.gene52518:intron | 40.0% | |
| TGAGAAGATTTGGGAAGACA+AGG | - | chr7.2:62696397-62696416 | MS.gene52518:intron | 40.0% | |
| TTTATGAGTCCTAGACCTGA+TGG | - | chr7.2:62694981-62695000 | MS.gene52518:intron | 40.0% | |
| TTTGTGCTTACTGCTACATC+TGG | - | chr7.2:62696778-62696797 | MS.gene52518:intron | 40.0% | |
| ! | ACCAAAGCTTCCCTTTTGTA+AGG | - | chr7.2:62696006-62696025 | MS.gene52518:intron | 40.0% |
| ! | CCAAAGCTTCCCTTTTGTAA+GGG | - | chr7.2:62696007-62696026 | MS.gene52518:intron | 40.0% |
| ! | GACTTTGCTATGATGATGTG+GGG | - | chr7.2:62696356-62696375 | MS.gene52518:intron | 40.0% |
| ! | GCTTTTAAATTTGTGGAGGC+TGG | - | chr7.2:62695058-62695077 | MS.gene52518:intron | 40.0% |
| !! | GATGGAGTTGGTATGTGTAT+CGG | - | chr7.2:62696656-62696675 | MS.gene52518:intron | 40.0% |
| !!! | TTTTTTTTCTGTGGCTGCAG+TGG | - | chr7.2:62696081-62696100 | MS.gene52518:intron | 40.0% |
| AAAAAAGTAAGTGAGGCGGC+AGG | - | chr7.2:62696237-62696256 | MS.gene52518:intron | 45.0% | |
| AAAAAGTAAGTGAGGCGGCA+GGG | - | chr7.2:62696238-62696257 | MS.gene52518:intron | 45.0% | |
| AACTTTGTCTCTAGGCTTGC+AGG | - | chr7.2:62694834-62694853 | MS.gene52518:CDS | 45.0% | |
| CCCTTACAAAAGGGAAGCTT+TGG | + | chr7.2:62696010-62696029 | None:intergenic | 45.0% | |
| GAACTCATTCCATCAGGTCT+AGG | + | chr7.2:62694993-62695012 | None:intergenic | 45.0% | |
| GAGAAAACAACAGACCAGGT+AGG | + | chr7.2:62695599-62695618 | None:intergenic | 45.0% | |
| GCAGATGGAACTTTGTCTCT+AGG | - | chr7.2:62694826-62694845 | MS.gene52518:CDS | 45.0% | |
| GCTGTGGACAAGATGAAAGA+AGG | - | chr7.2:62696137-62696156 | MS.gene52518:intron | 45.0% | |
| GTAGGGAAAAGTTGCATGGT+AGG | - | chr7.2:62695719-62695738 | MS.gene52518:intron | 45.0% | |
| GTTCTTCCTCCACTTTACTG+AGG | + | chr7.2:62695461-62695480 | None:intergenic | 45.0% | |
| TCACGCAATGGAAGGAGATT+AGG | - | chr7.2:62696460-62696479 | MS.gene52518:intron | 45.0% | |
| TTGTCCACAGCTTCAAGCAA+AGG | + | chr7.2:62696128-62696147 | None:intergenic | 45.0% | |
| TTTACAGTTCAGCAGTACGG+TGG | - | chr7.2:62696567-62696586 | MS.gene52518:intron | 45.0% | |
| AACAGACCAGGTAGGAGTCA+AGG | + | chr7.2:62695591-62695610 | None:intergenic | 50.0% | |
| AGCAGACTGTAGCAGCGAAT+AGG | - | chr7.2:62696484-62696503 | MS.gene52518:intron | 50.0% | |
| AGTACGGTGGATGCCACTAA+TGG | - | chr7.2:62696580-62696599 | MS.gene52518:intron | 50.0% | |
| CGAGACTATCACGCAATGGA+AGG | - | chr7.2:62696452-62696471 | MS.gene52518:intron | 50.0% | |
| GGAAGACCTCAGTAAAGTGG+AGG | - | chr7.2:62695452-62695471 | MS.gene52518:CDS | 50.0% | |
| GGAGGAAGACCTCAGTAAAG+TGG | - | chr7.2:62695449-62695468 | MS.gene52518:CDS | 50.0% | |
| TGCACGAGACTATCACGCAA+TGG | - | chr7.2:62696448-62696467 | MS.gene52518:intron | 50.0% | |
| !! | TTTCAAGAAGAGCGCTGCGA+TGG | - | chr7.2:62696638-62696657 | MS.gene52518:intron | 50.0% |
| CGGTGGATGCCACTAATGGT+AGG | - | chr7.2:62696584-62696603 | MS.gene52518:intron | 55.0% | |
| GGAGTCAAGGCCAATGACCT+TGG | + | chr7.2:62695578-62695597 | None:intergenic | 55.0% | |
| !! | CATTGGCCTTGACTCCTACC+TGG | - | chr7.2:62695582-62695601 | MS.gene52518:CDS | 55.0% |
| TTCTGTGGCTGCAGTGGCTG+TGG | - | chr7.2:62696087-62696106 | MS.gene52518:intron | 60.0% | |
| !! | GAAGAGCGCTGCGATGGAGT+TGG | - | chr7.2:62696644-62696663 | MS.gene52518:intron | 60.0% |
| AAGTGAGGCGGCAGGGCCGT+GGG | - | chr7.2:62696245-62696264 | MS.gene52518:intron | 70.0% | |
| TAAGTGAGGCGGCAGGGCCG+TGG | - | chr7.2:62696244-62696263 | MS.gene52518:intron | 70.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 62694817 | 62696903 | 62694817 | ID=MS.gene52518 |
| chr7.2 | mRNA | 62694817 | 62696903 | 62694817 | ID=MS.gene52518.t1;Parent=MS.gene52518 |
| chr7.2 | exon | 62696865 | 62696903 | 62696865 | ID=MS.gene52518.t1.exon1;Parent=MS.gene52518.t1 |
| chr7.2 | CDS | 62696865 | 62696903 | 62696865 | ID=cds.MS.gene52518.t1;Parent=MS.gene52518.t1 |
| chr7.2 | exon | 62695562 | 62695619 | 62695562 | ID=MS.gene52518.t1.exon2;Parent=MS.gene52518.t1 |
| chr7.2 | CDS | 62695562 | 62695619 | 62695562 | ID=cds.MS.gene52518.t1;Parent=MS.gene52518.t1 |
| chr7.2 | exon | 62695428 | 62695488 | 62695428 | ID=MS.gene52518.t1.exon3;Parent=MS.gene52518.t1 |
| chr7.2 | CDS | 62695428 | 62695488 | 62695428 | ID=cds.MS.gene52518.t1;Parent=MS.gene52518.t1 |
| chr7.2 | exon | 62695193 | 62695334 | 62695193 | ID=MS.gene52518.t1.exon4;Parent=MS.gene52518.t1 |
| chr7.2 | CDS | 62695193 | 62695334 | 62695193 | ID=cds.MS.gene52518.t1;Parent=MS.gene52518.t1 |
| chr7.2 | exon | 62694817 | 62694888 | 62694817 | ID=MS.gene52518.t1.exon5;Parent=MS.gene52518.t1 |
| chr7.2 | CDS | 62694817 | 62694888 | 62694817 | ID=cds.MS.gene52518.t1;Parent=MS.gene52518.t1 |
| Gene Sequence |
| Protein sequence |
