Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52570.t1 | XP_003624171.2 | 100 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 1.60E-50 | 208.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52570.t1 | Q9FFD8 | 76.2 | 101 | 22 | 1 | 1 | 101 | 1 | 99 | 1.4e-39 | 163.3 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52570.t1 | G7KRI0 | 100.0 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 1.1e-50 | 208.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058726 | MS.gene52570 | 0.960223 | 2.58E-118 | -1.69E-46 |
| MS.gene058728 | MS.gene52570 | 0.929274 | 8.79E-93 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52570.t1 | MTR_7g079990 | 100.000 | 101 | 0 | 0 | 1 | 101 | 1 | 101 | 3.82e-70 | 204 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52570.t1 | AT5G16470 | 76.238 | 101 | 22 | 1 | 1 | 101 | 1 | 99 | 1.39e-52 | 160 |
| MS.gene52570.t1 | AT5G16470 | 76.238 | 101 | 22 | 1 | 1 | 101 | 1 | 99 | 1.39e-52 | 160 |
| MS.gene52570.t1 | AT3G02790 | 76.238 | 101 | 23 | 1 | 1 | 101 | 1 | 100 | 4.55e-52 | 159 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGCCGTGTGCTTCTT+TGG | 0.219018 | 7.4:-26137880 | None:intergenic |
| TCTTCCTCAAAAGGGATCTT+TGG | 0.247762 | 7.4:-26138072 | None:intergenic |
| CGTCAGCGCCGCGTCAACCT+TGG | 0.424775 | 7.4:-26137911 | None:intergenic |
| ATCTGCATCGATTTCACATC+AGG | 0.442800 | 7.4:-26138030 | None:intergenic |
| GCCAAAGAAGCACACGGCGA+AGG | 0.455639 | 7.4:+26137879 | MS.gene52570:CDS |
| GAAAAGCCGGTTTGCTTGAT+CGG | 0.458319 | 7.4:+26137947 | MS.gene52570:CDS |
| AAGCCTCGCCCTGGTGTTCG+TGG | 0.465707 | 7.4:+26138144 | MS.gene52570:CDS |
| ACGAACACCAGGGCGAGGCT+TGG | 0.472106 | 7.4:-26138142 | None:intergenic |
| TGATCGGATCGGTGTTGAAA+AGG | 0.486280 | 7.4:+26137963 | MS.gene52570:CDS |
| GCGCCGCGTCAACCTTGGCT+TGG | 0.491264 | 7.4:-26137906 | None:intergenic |
| CGAGGCTTGGAGGATTCAGC+AGG | 0.496573 | 7.4:-26138129 | None:intergenic |
| AACACCAGGGCGAGGCTTGG+AGG | 0.501344 | 7.4:-26138139 | None:intergenic |
| GAATCCTCCAAGCCTCGCCC+TGG | 0.508082 | 7.4:+26138135 | MS.gene52570:CDS |
| ACCGATCCGATCAAGCAAAC+CGG | 0.511341 | 7.4:-26137953 | None:intergenic |
| GCGCTGACGAATCGCGGCGG+AGG | 0.512793 | 7.4:+26137925 | MS.gene52570:CDS |
| GCAGTGAGGGCACTCCCACT+TGG | 0.515764 | 7.4:-26137995 | None:intergenic |
| TTGAGACTACCACGAACACC+AGG | 0.527196 | 7.4:-26138153 | None:intergenic |
| GACGCGGCGCTGACGAATCG+CGG | 0.534103 | 7.4:+26137919 | MS.gene52570:CDS |
| GATCGGATCGGTGTTGAAAA+GGG | 0.542506 | 7.4:+26137964 | MS.gene52570:CDS |
| CAATCATCAATCAAGATGAC+GGG | 0.549212 | 7.4:+26137847 | None:intergenic |
| AATCGCGGCGGAGGAAAAGC+CGG | 0.554621 | 7.4:+26137934 | MS.gene52570:CDS |
| GCCGGTTTGCTTGATCGGAT+CGG | 0.570802 | 7.4:+26137952 | MS.gene52570:CDS |
| CTACCACGAACACCAGGGCG+AGG | 0.597449 | 7.4:-26138147 | None:intergenic |
| CGGATCGGTGTTGAAAAGGG+TGG | 0.630433 | 7.4:+26137967 | MS.gene52570:CDS |
| GGCGAAGGAGATCCAAGCCA+AGG | 0.648739 | 7.4:+26137894 | MS.gene52570:CDS |
| AGCGAAGCCAAAGAAGCACA+CGG | 0.650014 | 7.4:+26137873 | MS.gene52570:CDS |
| GATCCAAGCCAAGGTTGACG+CGG | 0.654184 | 7.4:+26137903 | MS.gene52570:CDS |
| AAAAGGGTGGACACGCCAAG+TGG | 0.671036 | 7.4:+26137980 | MS.gene52570:CDS |
| GCGGCGCTGACGAATCGCGG+CGG | 0.674143 | 7.4:+26137922 | MS.gene52570:CDS |
| AAAGGGTGGACACGCCAAGT+GGG | 0.690189 | 7.4:+26137981 | MS.gene52570:CDS |
| TGAGACTACCACGAACACCA+GGG | 0.697182 | 7.4:-26138152 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGCTTTTCTTCCTCAAA+AGG | - | chr7.4:26138084-26138103 | None:intergenic | 35.0% |
| ! | CAAGCTTTTCTTCCTCAAAA+GGG | - | chr7.4:26138083-26138102 | None:intergenic | 35.0% |
| TCTTCCTCAAAAGGGATCTT+TGG | - | chr7.4:26138075-26138094 | None:intergenic | 40.0% | |
| !! | ATCTGCATCGATTTCACATC+AGG | - | chr7.4:26138033-26138052 | None:intergenic | 40.0% |
| ! | GCATCCAAAGATCCCTTTTG+AGG | + | chr7.4:26138068-26138087 | MS.gene52570:CDS | 45.0% |
| !! | GAAAAGCCGGTTTGCTTGAT+CGG | + | chr7.4:26137947-26137966 | MS.gene52570:CDS | 45.0% |
| !! | GATCGGATCGGTGTTGAAAA+GGG | + | chr7.4:26137964-26137983 | MS.gene52570:CDS | 45.0% |
| !! | TGATCGGATCGGTGTTGAAA+AGG | + | chr7.4:26137963-26137982 | MS.gene52570:CDS | 45.0% |
| ACCGATCCGATCAAGCAAAC+CGG | - | chr7.4:26137956-26137975 | None:intergenic | 50.0% | |
| ! | AGCGAAGCCAAAGAAGCACA+CGG | + | chr7.4:26137873-26137892 | MS.gene52570:CDS | 50.0% |
| ! | GCGCTGTGATTTTGCAGTGA+GGG | - | chr7.4:26138011-26138030 | None:intergenic | 50.0% |
| AAAAGGGTGGACACGCCAAG+TGG | + | chr7.4:26137980-26137999 | MS.gene52570:CDS | 55.0% | |
| AAAGGGTGGACACGCCAAGT+GGG | + | chr7.4:26137981-26138000 | MS.gene52570:CDS | 55.0% | |
| GATCCAAGCCAAGGTTGACG+CGG | + | chr7.4:26137903-26137922 | MS.gene52570:CDS | 55.0% | |
| TCCTTCGCCGTGTGCTTCTT+TGG | - | chr7.4:26137883-26137902 | None:intergenic | 55.0% | |
| ! | GGCGCTGTGATTTTGCAGTG+AGG | - | chr7.4:26138012-26138031 | None:intergenic | 55.0% |
| !! | CGGATCGGTGTTGAAAAGGG+TGG | + | chr7.4:26137967-26137986 | MS.gene52570:CDS | 55.0% |
| !! | GCCGGTTTGCTTGATCGGAT+CGG | + | chr7.4:26137952-26137971 | MS.gene52570:CDS | 55.0% |
| AATCGCGGCGGAGGAAAAGC+CGG | + | chr7.4:26137934-26137953 | MS.gene52570:CDS | 60.0% | |
| CGAGGCTTGGAGGATTCAGC+AGG | - | chr7.4:26138132-26138151 | None:intergenic | 60.0% | |
| GGCGAAGGAGATCCAAGCCA+AGG | + | chr7.4:26137894-26137913 | MS.gene52570:CDS | 60.0% | |
| ! | GCCAAAGAAGCACACGGCGA+AGG | + | chr7.4:26137879-26137898 | MS.gene52570:CDS | 60.0% |
| AACACCAGGGCGAGGCTTGG+AGG | - | chr7.4:26138142-26138161 | None:intergenic | 65.0% | |
| ACGAACACCAGGGCGAGGCT+TGG | - | chr7.4:26138145-26138164 | None:intergenic | 65.0% | |
| GAATCCTCCAAGCCTCGCCC+TGG | + | chr7.4:26138135-26138154 | MS.gene52570:CDS | 65.0% | |
| !! | GCAGTGAGGGCACTCCCACT+TGG | - | chr7.4:26137998-26138017 | None:intergenic | 65.0% |
| CGTCAGCGCCGCGTCAACCT+TGG | - | chr7.4:26137914-26137933 | None:intergenic | 70.0% | |
| GCGCCGCGTCAACCTTGGCT+TGG | - | chr7.4:26137909-26137928 | None:intergenic | 70.0% | |
| !! | GACGCGGCGCTGACGAATCG+CGG | + | chr7.4:26137919-26137938 | MS.gene52570:CDS | 70.0% |
| ! | GCGCTGACGAATCGCGGCGG+AGG | + | chr7.4:26137925-26137944 | MS.gene52570:CDS | 75.0% |
| !! | GCGGCGCTGACGAATCGCGG+CGG | + | chr7.4:26137922-26137941 | MS.gene52570:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26137862 | 26138165 | 26137862 | ID=MS.gene52570 |
| chr7.4 | mRNA | 26137862 | 26138165 | 26137862 | ID=MS.gene52570.t1;Parent=MS.gene52570 |
| chr7.4 | exon | 26137862 | 26138165 | 26137862 | ID=MS.gene52570.t1.exon1;Parent=MS.gene52570.t1 |
| chr7.4 | CDS | 26137862 | 26138165 | 26137862 | ID=cds.MS.gene52570.t1;Parent=MS.gene52570.t1 |
| Gene Sequence |
| Protein sequence |
