Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058726.t1 | XP_003624171.2 | 100 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 3.60E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058726.t1 | Q9FFD8 | 77.4 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 4.1e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058726.t1 | G7KRI0 | 100.0 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 2.6e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058726 | MS.gene058728 | 0.974492 | 2.98E-138 | -1.69E-46 |
| MS.gene058726 | MS.gene07408 | -0.820539 | 6.50E-53 | -1.69E-46 |
| MS.gene058726 | MS.gene47377 | -0.810998 | 8.72E-51 | -1.69E-46 |
| MS.gene058726 | MS.gene49263 | 0.813762 | 2.17E-51 | -1.69E-46 |
| MS.gene058726 | MS.gene52570 | 0.960223 | 2.58E-118 | -1.69E-46 |
| MS.gene058726 | MS.gene52572 | 0.919692 | 3.28E-87 | -1.69E-46 |
| MS.gene058726 | MS.gene52575 | 0.985415 | 1.70E-163 | -1.69E-46 |
| MS.gene058726 | MS.gene72257 | -0.817338 | 3.47E-52 | -1.69E-46 |
| MS.gene058726 | MS.gene96863 | -0.814103 | 1.83E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058726.t1 | MTR_7g079990 | 100.000 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 1.71e-73 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058726.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene058726.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene058726.t1 | AT3G02790 | 77.358 | 106 | 23 | 1 | 1 | 106 | 1 | 105 | 2.44e-55 | 167 |
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGCCGTGTGCTTCTT+TGG | 0.219018 | 7.4:+26203616 | None:intergenic |
| TCTTCCTCAAAAGGGATCTT+TGG | 0.247762 | 7.4:+26203424 | None:intergenic |
| CGTCAGCGCCGCGTCAACCT+TGG | 0.424775 | 7.4:+26203585 | None:intergenic |
| ATCTGCATCGATTTCACATC+AGG | 0.442800 | 7.4:+26203466 | None:intergenic |
| GCCAAAGAAGCACACGGCGA+AGG | 0.455639 | 7.4:-26203617 | MS.gene058726:CDS |
| GAAAAGCCGGTTTGCTTGAT+CGG | 0.458319 | 7.4:-26203549 | MS.gene058726:CDS |
| AAGCCTCGCCCTGGTGTTCG+TGG | 0.465707 | 7.4:-26203352 | MS.gene058726:CDS |
| ACGAACACCAGGGCGAGGCT+TGG | 0.472106 | 7.4:+26203354 | None:intergenic |
| TGATCGGATCGGTGTTGAAA+AGG | 0.486280 | 7.4:-26203533 | MS.gene058726:CDS |
| GCGCCGCGTCAACCTTGGCT+TGG | 0.491264 | 7.4:+26203590 | None:intergenic |
| CGAGGCTTGGAGGATTCAGC+AGG | 0.496573 | 7.4:+26203367 | None:intergenic |
| AACACCAGGGCGAGGCTTGG+AGG | 0.501344 | 7.4:+26203357 | None:intergenic |
| GAATCCTCCAAGCCTCGCCC+TGG | 0.508082 | 7.4:-26203361 | MS.gene058726:CDS |
| ACCGATCCGATCAAGCAAAC+CGG | 0.511341 | 7.4:+26203543 | None:intergenic |
| GCGCTGACGAATCGCGGCGG+AGG | 0.512793 | 7.4:-26203571 | MS.gene058726:CDS |
| GCAGTGAGGGCACTCCCACT+TGG | 0.515764 | 7.4:+26203501 | None:intergenic |
| TTGAGACTACCACGAACACC+AGG | 0.527196 | 7.4:+26203343 | None:intergenic |
| GACGCGGCGCTGACGAATCG+CGG | 0.534103 | 7.4:-26203577 | MS.gene058726:CDS |
| GATCGGATCGGTGTTGAAAA+GGG | 0.542506 | 7.4:-26203532 | MS.gene058726:CDS |
| CAATCATCAATCAAGATGAC+GGG | 0.549212 | 7.4:-26203649 | None:intergenic |
| AATCGCGGCGGAGGAAAAGC+CGG | 0.554621 | 7.4:-26203562 | MS.gene058726:CDS |
| GCCGGTTTGCTTGATCGGAT+CGG | 0.570802 | 7.4:-26203544 | MS.gene058726:CDS |
| CTACCACGAACACCAGGGCG+AGG | 0.597449 | 7.4:+26203349 | None:intergenic |
| CGGATCGGTGTTGAAAAGGG+TGG | 0.630433 | 7.4:-26203529 | MS.gene058726:CDS |
| GGCGAAGGAGATCCAAGCCA+AGG | 0.648739 | 7.4:-26203602 | MS.gene058726:CDS |
| AGCGAAGCCAAAGAAGCACA+CGG | 0.650014 | 7.4:-26203623 | MS.gene058726:CDS |
| GATCCAAGCCAAGGTTGACG+CGG | 0.654184 | 7.4:-26203593 | MS.gene058726:CDS |
| AAAAGGGTGGACACGCCAAG+TGG | 0.671036 | 7.4:-26203516 | MS.gene058726:CDS |
| GCGGCGCTGACGAATCGCGG+CGG | 0.674143 | 7.4:-26203574 | MS.gene058726:CDS |
| AAAGGGTGGACACGCCAAGT+GGG | 0.690189 | 7.4:-26203515 | MS.gene058726:CDS |
| TGAGACTACCACGAACACCA+GGG | 0.697182 | 7.4:+26203344 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGCTTTTCTTCCTCAAA+AGG | + | chr7.4:26203558-26203577 | None:intergenic | 35.0% |
| ! | CAAGCTTTTCTTCCTCAAAA+GGG | + | chr7.4:26203557-26203576 | None:intergenic | 35.0% |
| TCTTCCTCAAAAGGGATCTT+TGG | + | chr7.4:26203549-26203568 | None:intergenic | 40.0% | |
| !! | ATCTGCATCGATTTCACATC+AGG | + | chr7.4:26203507-26203526 | None:intergenic | 40.0% |
| ! | GCATCCAAAGATCCCTTTTG+AGG | - | chr7.4:26203542-26203561 | MS.gene058726:CDS | 45.0% |
| !! | GAAAAGCCGGTTTGCTTGAT+CGG | - | chr7.4:26203421-26203440 | MS.gene058726:CDS | 45.0% |
| !! | GATCGGATCGGTGTTGAAAA+GGG | - | chr7.4:26203438-26203457 | MS.gene058726:CDS | 45.0% |
| !! | TGATCGGATCGGTGTTGAAA+AGG | - | chr7.4:26203437-26203456 | MS.gene058726:CDS | 45.0% |
| ACCGATCCGATCAAGCAAAC+CGG | + | chr7.4:26203430-26203449 | None:intergenic | 50.0% | |
| TGAGACTACCACGAACACCA+GGG | + | chr7.4:26203629-26203648 | None:intergenic | 50.0% | |
| TTGAGACTACCACGAACACC+AGG | + | chr7.4:26203630-26203649 | None:intergenic | 50.0% | |
| ! | AGCGAAGCCAAAGAAGCACA+CGG | - | chr7.4:26203347-26203366 | MS.gene058726:CDS | 50.0% |
| ! | GCGCTGTGATTTTGCAGTGA+GGG | + | chr7.4:26203485-26203504 | None:intergenic | 50.0% |
| AAAAGGGTGGACACGCCAAG+TGG | - | chr7.4:26203454-26203473 | MS.gene058726:CDS | 55.0% | |
| AAAGGGTGGACACGCCAAGT+GGG | - | chr7.4:26203455-26203474 | MS.gene058726:CDS | 55.0% | |
| GATCCAAGCCAAGGTTGACG+CGG | - | chr7.4:26203377-26203396 | MS.gene058726:CDS | 55.0% | |
| TCCTTCGCCGTGTGCTTCTT+TGG | + | chr7.4:26203357-26203376 | None:intergenic | 55.0% | |
| ! | GGCGCTGTGATTTTGCAGTG+AGG | + | chr7.4:26203486-26203505 | None:intergenic | 55.0% |
| !! | CGGATCGGTGTTGAAAAGGG+TGG | - | chr7.4:26203441-26203460 | MS.gene058726:CDS | 55.0% |
| !! | GCCGGTTTGCTTGATCGGAT+CGG | - | chr7.4:26203426-26203445 | MS.gene058726:CDS | 55.0% |
| AATCGCGGCGGAGGAAAAGC+CGG | - | chr7.4:26203408-26203427 | MS.gene058726:CDS | 60.0% | |
| CGAGGCTTGGAGGATTCAGC+AGG | + | chr7.4:26203606-26203625 | None:intergenic | 60.0% | |
| GGCGAAGGAGATCCAAGCCA+AGG | - | chr7.4:26203368-26203387 | MS.gene058726:CDS | 60.0% | |
| ! | GCCAAAGAAGCACACGGCGA+AGG | - | chr7.4:26203353-26203372 | MS.gene058726:CDS | 60.0% |
| AACACCAGGGCGAGGCTTGG+AGG | + | chr7.4:26203616-26203635 | None:intergenic | 65.0% | |
| AAGCCTCGCCCTGGTGTTCG+TGG | - | chr7.4:26203618-26203637 | MS.gene058726:CDS | 65.0% | |
| ACGAACACCAGGGCGAGGCT+TGG | + | chr7.4:26203619-26203638 | None:intergenic | 65.0% | |
| CTACCACGAACACCAGGGCG+AGG | + | chr7.4:26203624-26203643 | None:intergenic | 65.0% | |
| GAATCCTCCAAGCCTCGCCC+TGG | - | chr7.4:26203609-26203628 | MS.gene058726:CDS | 65.0% | |
| !! | GCAGTGAGGGCACTCCCACT+TGG | + | chr7.4:26203472-26203491 | None:intergenic | 65.0% |
| CGTCAGCGCCGCGTCAACCT+TGG | + | chr7.4:26203388-26203407 | None:intergenic | 70.0% | |
| GCGCCGCGTCAACCTTGGCT+TGG | + | chr7.4:26203383-26203402 | None:intergenic | 70.0% | |
| !! | GACGCGGCGCTGACGAATCG+CGG | - | chr7.4:26203393-26203412 | MS.gene058726:CDS | 70.0% |
| ! | GCGCTGACGAATCGCGGCGG+AGG | - | chr7.4:26203399-26203418 | MS.gene058726:CDS | 75.0% |
| !! | GCGGCGCTGACGAATCGCGG+CGG | - | chr7.4:26203396-26203415 | MS.gene058726:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26203336 | 26203656 | 26203336 | ID=MS.gene058726 |
| chr7.4 | mRNA | 26203336 | 26203656 | 26203336 | ID=MS.gene058726.t1;Parent=MS.gene058726 |
| chr7.4 | exon | 26203336 | 26203656 | 26203336 | ID=MS.gene058726.t1.exon1;Parent=MS.gene058726.t1 |
| chr7.4 | CDS | 26203336 | 26203656 | 26203336 | ID=cds.MS.gene058726.t1;Parent=MS.gene058726.t1 |
| Gene Sequence |
| Protein sequence |
