Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058728.t1 | XP_003624171.2 | 100 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 3.60E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058728.t1 | Q9FFD8 | 77.4 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 4.1e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058728.t1 | G7KRI0 | 100.0 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 2.6e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058726 | MS.gene058728 | 0.974492 | 2.98E-138 | -1.69E-46 |
| MS.gene058728 | MS.gene07408 | -0.802972 | 4.35E-49 | -1.69E-46 |
| MS.gene058728 | MS.gene52570 | 0.929274 | 8.79E-93 | -1.69E-46 |
| MS.gene058728 | MS.gene52572 | 0.942442 | 7.14E-102 | -1.69E-46 |
| MS.gene058728 | MS.gene52575 | 0.9579 | 8.84E-116 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058728.t1 | MTR_7g079990 | 100.000 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 1.71e-73 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene058728.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene058728.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene058728.t1 | AT3G02790 | 77.358 | 106 | 23 | 1 | 1 | 106 | 1 | 105 | 2.44e-55 | 167 |
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGCCGTGTGCTTCTT+TGG | 0.219018 | 7.4:+26162142 | None:intergenic |
| TCTTCCTCAAAAGGGATCTT+TGG | 0.247762 | 7.4:+26161950 | None:intergenic |
| CGTCAGCGCCGCGTCAACCT+TGG | 0.424775 | 7.4:+26162111 | None:intergenic |
| ATCTGCATCGATTTCACATC+AGG | 0.442800 | 7.4:+26161992 | None:intergenic |
| GCCAAAGAAGCACACGGCGA+AGG | 0.455639 | 7.4:-26162143 | MS.gene058728:CDS |
| GAAAAGCCGGTTTGCTTGAT+CGG | 0.458319 | 7.4:-26162075 | MS.gene058728:CDS |
| AAGCCTCGCCCTGGTGTTCG+TGG | 0.465707 | 7.4:-26161878 | MS.gene058728:CDS |
| ACGAACACCAGGGCGAGGCT+TGG | 0.472106 | 7.4:+26161880 | None:intergenic |
| TGATCGGATCGGTGTTGAAA+AGG | 0.486280 | 7.4:-26162059 | MS.gene058728:CDS |
| GCGCCGCGTCAACCTTGGCT+TGG | 0.491264 | 7.4:+26162116 | None:intergenic |
| CGAGGCTTGGAGGATTCAGC+AGG | 0.496573 | 7.4:+26161893 | None:intergenic |
| AACACCAGGGCGAGGCTTGG+AGG | 0.501344 | 7.4:+26161883 | None:intergenic |
| GAATCCTCCAAGCCTCGCCC+TGG | 0.508082 | 7.4:-26161887 | MS.gene058728:CDS |
| ACCGATCCGATCAAGCAAAC+CGG | 0.511341 | 7.4:+26162069 | None:intergenic |
| GCGCTGACGAATCGCGGCGG+AGG | 0.512793 | 7.4:-26162097 | MS.gene058728:CDS |
| GCAGTGAGGGCACTCCCACT+TGG | 0.515764 | 7.4:+26162027 | None:intergenic |
| TTGAGACTACCACGAACACC+AGG | 0.527196 | 7.4:+26161869 | None:intergenic |
| GACGCGGCGCTGACGAATCG+CGG | 0.534103 | 7.4:-26162103 | MS.gene058728:CDS |
| GATCGGATCGGTGTTGAAAA+GGG | 0.542506 | 7.4:-26162058 | MS.gene058728:CDS |
| CAATCATCAATCAAGATGAC+GGG | 0.549212 | 7.4:-26162175 | None:intergenic |
| AATCGCGGCGGAGGAAAAGC+CGG | 0.554621 | 7.4:-26162088 | MS.gene058728:CDS |
| GCCGGTTTGCTTGATCGGAT+CGG | 0.570802 | 7.4:-26162070 | MS.gene058728:CDS |
| CTACCACGAACACCAGGGCG+AGG | 0.597449 | 7.4:+26161875 | None:intergenic |
| CGGATCGGTGTTGAAAAGGG+TGG | 0.630433 | 7.4:-26162055 | MS.gene058728:CDS |
| GGCGAAGGAGATCCAAGCCA+AGG | 0.648739 | 7.4:-26162128 | MS.gene058728:CDS |
| AGCGAAGCCAAAGAAGCACA+CGG | 0.650014 | 7.4:-26162149 | MS.gene058728:CDS |
| GATCCAAGCCAAGGTTGACG+CGG | 0.654184 | 7.4:-26162119 | MS.gene058728:CDS |
| AAAAGGGTGGACACGCCAAG+TGG | 0.671036 | 7.4:-26162042 | MS.gene058728:CDS |
| GCGGCGCTGACGAATCGCGG+CGG | 0.674143 | 7.4:-26162100 | MS.gene058728:CDS |
| AAAGGGTGGACACGCCAAGT+GGG | 0.690189 | 7.4:-26162041 | MS.gene058728:CDS |
| TGAGACTACCACGAACACCA+GGG | 0.697182 | 7.4:+26161870 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGCTTTTCTTCCTCAAA+AGG | + | chr7.4:26162084-26162103 | None:intergenic | 35.0% |
| ! | CAAGCTTTTCTTCCTCAAAA+GGG | + | chr7.4:26162083-26162102 | None:intergenic | 35.0% |
| TCTTCCTCAAAAGGGATCTT+TGG | + | chr7.4:26162075-26162094 | None:intergenic | 40.0% | |
| !! | ATCTGCATCGATTTCACATC+AGG | + | chr7.4:26162033-26162052 | None:intergenic | 40.0% |
| ! | GCATCCAAAGATCCCTTTTG+AGG | - | chr7.4:26162068-26162087 | MS.gene058728:CDS | 45.0% |
| !! | GAAAAGCCGGTTTGCTTGAT+CGG | - | chr7.4:26161947-26161966 | MS.gene058728:CDS | 45.0% |
| !! | GATCGGATCGGTGTTGAAAA+GGG | - | chr7.4:26161964-26161983 | MS.gene058728:CDS | 45.0% |
| !! | TGATCGGATCGGTGTTGAAA+AGG | - | chr7.4:26161963-26161982 | MS.gene058728:CDS | 45.0% |
| ACCGATCCGATCAAGCAAAC+CGG | + | chr7.4:26161956-26161975 | None:intergenic | 50.0% | |
| TGAGACTACCACGAACACCA+GGG | + | chr7.4:26162155-26162174 | None:intergenic | 50.0% | |
| TTGAGACTACCACGAACACC+AGG | + | chr7.4:26162156-26162175 | None:intergenic | 50.0% | |
| ! | AGCGAAGCCAAAGAAGCACA+CGG | - | chr7.4:26161873-26161892 | MS.gene058728:CDS | 50.0% |
| ! | GCGCTGTGATTTTGCAGTGA+GGG | + | chr7.4:26162011-26162030 | None:intergenic | 50.0% |
| AAAAGGGTGGACACGCCAAG+TGG | - | chr7.4:26161980-26161999 | MS.gene058728:CDS | 55.0% | |
| AAAGGGTGGACACGCCAAGT+GGG | - | chr7.4:26161981-26162000 | MS.gene058728:CDS | 55.0% | |
| GATCCAAGCCAAGGTTGACG+CGG | - | chr7.4:26161903-26161922 | MS.gene058728:CDS | 55.0% | |
| TCCTTCGCCGTGTGCTTCTT+TGG | + | chr7.4:26161883-26161902 | None:intergenic | 55.0% | |
| ! | GGCGCTGTGATTTTGCAGTG+AGG | + | chr7.4:26162012-26162031 | None:intergenic | 55.0% |
| !! | CGGATCGGTGTTGAAAAGGG+TGG | - | chr7.4:26161967-26161986 | MS.gene058728:CDS | 55.0% |
| !! | GCCGGTTTGCTTGATCGGAT+CGG | - | chr7.4:26161952-26161971 | MS.gene058728:CDS | 55.0% |
| AATCGCGGCGGAGGAAAAGC+CGG | - | chr7.4:26161934-26161953 | MS.gene058728:CDS | 60.0% | |
| CGAGGCTTGGAGGATTCAGC+AGG | + | chr7.4:26162132-26162151 | None:intergenic | 60.0% | |
| GGCGAAGGAGATCCAAGCCA+AGG | - | chr7.4:26161894-26161913 | MS.gene058728:CDS | 60.0% | |
| ! | GCCAAAGAAGCACACGGCGA+AGG | - | chr7.4:26161879-26161898 | MS.gene058728:CDS | 60.0% |
| AACACCAGGGCGAGGCTTGG+AGG | + | chr7.4:26162142-26162161 | None:intergenic | 65.0% | |
| AAGCCTCGCCCTGGTGTTCG+TGG | - | chr7.4:26162144-26162163 | MS.gene058728:CDS | 65.0% | |
| ACGAACACCAGGGCGAGGCT+TGG | + | chr7.4:26162145-26162164 | None:intergenic | 65.0% | |
| CTACCACGAACACCAGGGCG+AGG | + | chr7.4:26162150-26162169 | None:intergenic | 65.0% | |
| GAATCCTCCAAGCCTCGCCC+TGG | - | chr7.4:26162135-26162154 | MS.gene058728:CDS | 65.0% | |
| !! | GCAGTGAGGGCACTCCCACT+TGG | + | chr7.4:26161998-26162017 | None:intergenic | 65.0% |
| CGTCAGCGCCGCGTCAACCT+TGG | + | chr7.4:26161914-26161933 | None:intergenic | 70.0% | |
| GCGCCGCGTCAACCTTGGCT+TGG | + | chr7.4:26161909-26161928 | None:intergenic | 70.0% | |
| !! | GACGCGGCGCTGACGAATCG+CGG | - | chr7.4:26161919-26161938 | MS.gene058728:CDS | 70.0% |
| ! | GCGCTGACGAATCGCGGCGG+AGG | - | chr7.4:26161925-26161944 | MS.gene058728:CDS | 75.0% |
| !! | GCGGCGCTGACGAATCGCGG+CGG | - | chr7.4:26161922-26161941 | MS.gene058728:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26161862 | 26162182 | 26161862 | ID=MS.gene058728 |
| chr7.4 | mRNA | 26161862 | 26162182 | 26161862 | ID=MS.gene058728.t1;Parent=MS.gene058728 |
| chr7.4 | exon | 26161862 | 26162182 | 26161862 | ID=MS.gene058728.t1.exon1;Parent=MS.gene058728.t1 |
| chr7.4 | CDS | 26161862 | 26162182 | 26161862 | ID=cds.MS.gene058728.t1;Parent=MS.gene058728.t1 |
| Gene Sequence |
| Protein sequence |
