Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52572.t1 | XP_003624171.2 | 88.5 | 104 | 12 | 0 | 35 | 138 | 3 | 106 | 8.80E-44 | 186.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52572.t1 | Q9FFD8 | 66.3 | 104 | 33 | 1 | 35 | 138 | 3 | 104 | 6.0e-33 | 141.7 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52572.t1 | G7KRI0 | 88.5 | 104 | 12 | 0 | 35 | 138 | 3 | 106 | 6.3e-44 | 186.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058726 | MS.gene52572 | 0.919692 | 3.28E-87 | -1.69E-46 |
| MS.gene058728 | MS.gene52572 | 0.942442 | 7.14E-102 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52572.t1 | MTR_7g079990 | 88.462 | 104 | 12 | 0 | 35 | 138 | 3 | 106 | 4.36e-61 | 183 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52572.t1 | AT5G16470 | 73.333 | 90 | 22 | 1 | 49 | 138 | 17 | 104 | 9.99e-44 | 139 |
| MS.gene52572.t1 | AT5G16470 | 73.333 | 90 | 22 | 1 | 49 | 138 | 17 | 104 | 9.99e-44 | 139 |
| MS.gene52572.t1 | AT3G02790 | 65.385 | 104 | 35 | 1 | 35 | 138 | 3 | 105 | 6.00e-43 | 137 |
Find 36 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGCCGTGTGCTTCTT+TGG | 0.219018 | 7.4:+26150982 | None:intergenic |
| TCTTCCTCAAAAGGGATCTT+TGG | 0.247762 | 7.4:+26150791 | None:intergenic |
| CCGTCATCTTGATTGATGAT+TGG | 0.363769 | 7.4:+26151016 | None:intergenic |
| CGTCATCTTGATTGATGATT+GGG | 0.380210 | 7.4:+26151017 | None:intergenic |
| TTTGGGGATTGAAACCCTAA+TGG | 0.438466 | 7.4:+26151076 | None:intergenic |
| AGCGCCGCGTCAACCTTGCT+TGG | 0.440776 | 7.4:+26150956 | None:intergenic |
| ATCTGCATCGATTTCACATC+AGG | 0.442800 | 7.4:+26150833 | None:intergenic |
| GCCAAAGAAGCACACGGCGA+AGG | 0.455639 | 7.4:-26150983 | MS.gene52572:CDS |
| GAAAAGCCGGTTTGCTTGAT+CGG | 0.458319 | 7.4:-26150916 | MS.gene52572:CDS |
| AAGCCTCGCCCTGGTGTTCG+TGG | 0.465707 | 7.4:-26150719 | MS.gene52572:CDS |
| ACGAACACCAGGGCGAGGCT+TGG | 0.472106 | 7.4:+26150721 | None:intergenic |
| TGATCGGATCGGTGTTGAAA+AGG | 0.486280 | 7.4:-26150900 | MS.gene52572:CDS |
| TTGGGGATTGAAACCCTAAT+GGG | 0.492402 | 7.4:+26151077 | None:intergenic |
| CGAGGCTTGGAGGATTCAGC+AGG | 0.496573 | 7.4:+26150734 | None:intergenic |
| AACACCAGGGCGAGGCTTGG+AGG | 0.501344 | 7.4:+26150724 | None:intergenic |
| GAATCCTCCAAGCCTCGCCC+TGG | 0.508082 | 7.4:-26150728 | MS.gene52572:CDS |
| ACCGATCCGATCAAGCAAAC+CGG | 0.511341 | 7.4:+26150910 | None:intergenic |
| GCGCTGACGAATCGCGGCGG+AGG | 0.512793 | 7.4:-26150938 | MS.gene52572:CDS |
| GCAGTGAGGGCACTCCCACT+TGG | 0.515764 | 7.4:+26150868 | None:intergenic |
| TTGAGACTACCACGAACACC+AGG | 0.527196 | 7.4:+26150710 | None:intergenic |
| GACGCGGCGCTGACGAATCG+CGG | 0.534103 | 7.4:-26150944 | MS.gene52572:CDS |
| ATGATTGGGTGAGAAATTTG+AGG | 0.537727 | 7.4:+26151031 | None:intergenic |
| GATCGGATCGGTGTTGAAAA+GGG | 0.542506 | 7.4:-26150899 | MS.gene52572:CDS |
| CAATCATCAATCAAGATGAC+GGG | 0.549212 | 7.4:-26151015 | MS.gene52572:CDS |
| AATCGCGGCGGAGGAAAAGC+CGG | 0.554621 | 7.4:-26150929 | MS.gene52572:CDS |
| GCCGGTTTGCTTGATCGGAT+CGG | 0.570802 | 7.4:-26150911 | MS.gene52572:CDS |
| CCAATCATCAATCAAGATGA+CGG | 0.575904 | 7.4:-26151016 | MS.gene52572:CDS |
| CTACCACGAACACCAGGGCG+AGG | 0.597449 | 7.4:+26150716 | None:intergenic |
| CGGATCGGTGTTGAAAAGGG+TGG | 0.630433 | 7.4:-26150896 | MS.gene52572:CDS |
| AGATCCAAGCAAGGTTGACG+CGG | 0.635434 | 7.4:-26150960 | MS.gene52572:CDS |
| AGCGAAGCCAAAGAAGCACA+CGG | 0.650014 | 7.4:-26150989 | MS.gene52572:CDS |
| AAAAGGGTGGACACGCCAAG+TGG | 0.671036 | 7.4:-26150883 | MS.gene52572:CDS |
| GCGGCGCTGACGAATCGCGG+CGG | 0.674143 | 7.4:-26150941 | MS.gene52572:CDS |
| AAAGGGTGGACACGCCAAGT+GGG | 0.690189 | 7.4:-26150882 | MS.gene52572:CDS |
| CGGCGAAGGAGATCCAAGCA+AGG | 0.692976 | 7.4:-26150969 | MS.gene52572:CDS |
| TGAGACTACCACGAACACCA+GGG | 0.697182 | 7.4:+26150711 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTTTATTTTTTAAATT+TGG | - | chr7.4:26150759-26150778 | MS.gene52572:CDS | 0.0% |
| !!! | TTTTTTTATTTTTTAAATTT+GGG | - | chr7.4:26150760-26150779 | MS.gene52572:CDS | 0.0% |
| !! | AAAAAATGTTATATTACAAA+AGG | + | chr7.4:26150709-26150728 | None:intergenic | 10.0% |
| !!! | TTAAGTTGTAAAAAAATATT+CGG | + | chr7.4:26150867-26150886 | None:intergenic | 10.0% |
| !! | ATATTTATAAATAGGAAAAC+GGG | + | chr7.4:26151121-26151140 | None:intergenic | 15.0% |
| !! | GATATTTATAAATAGGAAAA+CGG | + | chr7.4:26151122-26151141 | None:intergenic | 15.0% |
| !! | TAAACTCGATATTTATAAAT+AGG | + | chr7.4:26151129-26151148 | None:intergenic | 15.0% |
| !!! | AAGCGTTAATAATTATTTTA+AGG | - | chr7.4:26151053-26151072 | MS.gene52572:CDS | 15.0% |
| !!! | AGCGTTAATAATTATTTTAA+GGG | - | chr7.4:26151054-26151073 | MS.gene52572:CDS | 15.0% |
| !!! | ATTGAATTTTGATTTGATTT+GGG | + | chr7.4:26151224-26151243 | None:intergenic | 15.0% |
| !! | AACATTTGACATATTAAACT+AGG | + | chr7.4:26150891-26150910 | None:intergenic | 20.0% |
| !! | ACATTTGACATATTAAACTA+GGG | + | chr7.4:26150890-26150909 | None:intergenic | 20.0% |
| !! | TTAATAAGAGTTTATAGGTT+GGG | + | chr7.4:26150809-26150828 | None:intergenic | 20.0% |
| !!! | GATTGAATTTTGATTTGATT+TGG | + | chr7.4:26151225-26151244 | None:intergenic | 20.0% |
| !!! | TTGAATTTTGATTTGATTTG+GGG | + | chr7.4:26151223-26151242 | None:intergenic | 20.0% |
| ! | AAAAATCTAGCAATTTCTCA+TGG | + | chr7.4:26150962-26150981 | None:intergenic | 25.0% |
| ! | AAAATCTAGCAATTTCTCAT+GGG | + | chr7.4:26150961-26150980 | None:intergenic | 25.0% |
| ! | CTTAATAAGAGTTTATAGGT+TGG | + | chr7.4:26150810-26150829 | None:intergenic | 25.0% |
| ! | GACTCTTAATAAGAGTTTAT+AGG | + | chr7.4:26150814-26150833 | None:intergenic | 25.0% |
| ! | TAATAAGAGTTTATAGGTTG+GGG | + | chr7.4:26150808-26150827 | None:intergenic | 25.0% |
| ! | TCTTTCTTATTCTTGGTTTA+GGG | - | chr7.4:26151171-26151190 | MS.gene52572:intron | 25.0% |
| ! | TTCTTTCTTATTCTTGGTTT+AGG | - | chr7.4:26151170-26151189 | MS.gene52572:intron | 25.0% |
| !!! | TAAGGGTTAATTTGCAAATA+GGG | - | chr7.4:26151071-26151090 | MS.gene52572:CDS | 25.0% |
| !!! | TTAAGGGTTAATTTGCAAAT+AGG | - | chr7.4:26151070-26151089 | MS.gene52572:CDS | 25.0% |
| AACGCTTTCTTTCTTATTCT+TGG | - | chr7.4:26151164-26151183 | MS.gene52572:intron | 30.0% | |
| !! | AAGGGTTAATTTGCAAATAG+GGG | - | chr7.4:26151072-26151091 | MS.gene52572:CDS | 30.0% |
| !! | AATAAGAAAGAAAGCGTTTG+AGG | + | chr7.4:26151162-26151181 | None:intergenic | 30.0% |
| ATGATTGGGTGAGAAATTTG+AGG | + | chr7.4:26151252-26151271 | None:intergenic | 35.0% | |
| CAATCATCAATCAAGATGAC+GGG | - | chr7.4:26151265-26151284 | MS.gene52572:intron | 35.0% | |
| CCAATCATCAATCAAGATGA+CGG | - | chr7.4:26151264-26151283 | MS.gene52572:intron | 35.0% | |
| TTATAAATAGGAAAACGGGC+TGG | + | chr7.4:26151117-26151136 | None:intergenic | 35.0% | |
| ! | ACAAGCTTTTCTTCCTCAAA+AGG | + | chr7.4:26151501-26151520 | None:intergenic | 35.0% |
| ! | AGAGTCTTATCTATCTGATG+TGG | - | chr7.4:26150828-26150847 | MS.gene52572:CDS | 35.0% |
| ! | CAAGCTTTTCTTCCTCAAAA+GGG | + | chr7.4:26151500-26151519 | None:intergenic | 35.0% |
| ! | CGTCATCTTGATTGATGATT+GGG | + | chr7.4:26151266-26151285 | None:intergenic | 35.0% |
| ! | GAGTCTTATCTATCTGATGT+GGG | - | chr7.4:26150829-26150848 | MS.gene52572:CDS | 35.0% |
| ! | TATAAATAGGAAAACGGGCT+GGG | + | chr7.4:26151116-26151135 | None:intergenic | 35.0% |
| TCTTCCTCAAAAGGGATCTT+TGG | + | chr7.4:26151492-26151511 | None:intergenic | 40.0% | |
| TTGGGGATTGAAACCCTAAT+GGG | + | chr7.4:26151206-26151225 | None:intergenic | 40.0% | |
| TTTGGGGATTGAAACCCTAA+TGG | + | chr7.4:26151207-26151226 | None:intergenic | 40.0% | |
| ! | AGGGTTTTCTTCACCCATTA+GGG | - | chr7.4:26151190-26151209 | MS.gene52572:intron | 40.0% |
| ! | CCGTCATCTTGATTGATGAT+TGG | + | chr7.4:26151267-26151286 | None:intergenic | 40.0% |
| ! | TAGGGTTTTCTTCACCCATT+AGG | - | chr7.4:26151189-26151208 | MS.gene52572:intron | 40.0% |
| !! | AAGAAAGAAAGCGTTTGAGG+TGG | + | chr7.4:26151159-26151178 | None:intergenic | 40.0% |
| !! | ATCTGCATCGATTTCACATC+AGG | + | chr7.4:26151450-26151469 | None:intergenic | 40.0% |
| AATGTTCTCGCGCTTAAGCA+CGG | - | chr7.4:26150905-26150924 | MS.gene52572:CDS | 45.0% | |
| ! | GCATCCAAAGATCCCTTTTG+AGG | - | chr7.4:26151485-26151504 | MS.gene52572:intron | 45.0% |
| !! | GAAAAGCCGGTTTGCTTGAT+CGG | - | chr7.4:26151364-26151383 | MS.gene52572:intron | 45.0% |
| !! | GATCGGATCGGTGTTGAAAA+GGG | - | chr7.4:26151381-26151400 | MS.gene52572:intron | 45.0% |
| !! | TGATCGGATCGGTGTTGAAA+AGG | - | chr7.4:26151380-26151399 | MS.gene52572:intron | 45.0% |
| !!! | TTTTTTATTTTTTAAATTTG+GGG | - | chr7.4:26150761-26150780 | MS.gene52572:CDS | 5.0% |
| AAGCACGGGTCAACATCTAG+TGG | - | chr7.4:26150920-26150939 | MS.gene52572:CDS | 50.0% | |
| ACCGATCCGATCAAGCAAAC+CGG | + | chr7.4:26151373-26151392 | None:intergenic | 50.0% | |
| AGATCCAAGCAAGGTTGACG+CGG | - | chr7.4:26151320-26151339 | MS.gene52572:intron | 50.0% | |
| ATGTTCTCGCGCTTAAGCAC+GGG | - | chr7.4:26150906-26150925 | MS.gene52572:CDS | 50.0% | |
| TGAGACTACCACGAACACCA+GGG | + | chr7.4:26151572-26151591 | None:intergenic | 50.0% | |
| TTGAGACTACCACGAACACC+AGG | + | chr7.4:26151573-26151592 | None:intergenic | 50.0% | |
| ! | AGCGAAGCCAAAGAAGCACA+CGG | - | chr7.4:26151291-26151310 | MS.gene52572:intron | 50.0% |
| ! | CGGGCTGGGATAAAGAGATA+AGG | + | chr7.4:26151102-26151121 | None:intergenic | 50.0% |
| ! | GCGCTGTGATTTTGCAGTGA+GGG | + | chr7.4:26151428-26151447 | None:intergenic | 50.0% |
| AAAAGGGTGGACACGCCAAG+TGG | - | chr7.4:26151397-26151416 | MS.gene52572:intron | 55.0% | |
| AAAGGGTGGACACGCCAAGT+GGG | - | chr7.4:26151398-26151417 | MS.gene52572:intron | 55.0% | |
| TCCTTCGCCGTGTGCTTCTT+TGG | + | chr7.4:26151301-26151320 | None:intergenic | 55.0% | |
| ! | GGCGCTGTGATTTTGCAGTG+AGG | + | chr7.4:26151429-26151448 | None:intergenic | 55.0% |
| !! | CGGATCGGTGTTGAAAAGGG+TGG | - | chr7.4:26151384-26151403 | MS.gene52572:intron | 55.0% |
| !! | GCCGGTTTGCTTGATCGGAT+CGG | - | chr7.4:26151369-26151388 | MS.gene52572:intron | 55.0% |
| AATCGCGGCGGAGGAAAAGC+CGG | - | chr7.4:26151351-26151370 | MS.gene52572:intron | 60.0% | |
| CGAGGCTTGGAGGATTCAGC+AGG | + | chr7.4:26151549-26151568 | None:intergenic | 60.0% | |
| CGGCGAAGGAGATCCAAGCA+AGG | - | chr7.4:26151311-26151330 | MS.gene52572:intron | 60.0% | |
| ! | GCCAAAGAAGCACACGGCGA+AGG | - | chr7.4:26151297-26151316 | MS.gene52572:intron | 60.0% |
| AACACCAGGGCGAGGCTTGG+AGG | + | chr7.4:26151559-26151578 | None:intergenic | 65.0% | |
| AAGCCTCGCCCTGGTGTTCG+TGG | - | chr7.4:26151561-26151580 | MS.gene52572:intron | 65.0% | |
| ACGAACACCAGGGCGAGGCT+TGG | + | chr7.4:26151562-26151581 | None:intergenic | 65.0% | |
| AGCGCCGCGTCAACCTTGCT+TGG | + | chr7.4:26151327-26151346 | None:intergenic | 65.0% | |
| CTACCACGAACACCAGGGCG+AGG | + | chr7.4:26151567-26151586 | None:intergenic | 65.0% | |
| GAATCCTCCAAGCCTCGCCC+TGG | - | chr7.4:26151552-26151571 | MS.gene52572:intron | 65.0% | |
| !! | GCAGTGAGGGCACTCCCACT+TGG | + | chr7.4:26151415-26151434 | None:intergenic | 65.0% |
| !! | GACGCGGCGCTGACGAATCG+CGG | - | chr7.4:26151336-26151355 | MS.gene52572:intron | 70.0% |
| ! | GCGCTGACGAATCGCGGCGG+AGG | - | chr7.4:26151342-26151361 | MS.gene52572:intron | 75.0% |
| !! | GCGGCGCTGACGAATCGCGG+CGG | - | chr7.4:26151339-26151358 | MS.gene52572:intron | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26150703 | 26151599 | 26150703 | ID=MS.gene52572 |
| chr7.4 | mRNA | 26150703 | 26151599 | 26150703 | ID=MS.gene52572.t1;Parent=MS.gene52572 |
| chr7.4 | exon | 26151591 | 26151599 | 26151591 | ID=MS.gene52572.t1.exon1;Parent=MS.gene52572.t1 |
| chr7.4 | CDS | 26151591 | 26151599 | 26151591 | ID=cds.MS.gene52572.t1;Parent=MS.gene52572.t1 |
| chr7.4 | exon | 26150703 | 26151110 | 26150703 | ID=MS.gene52572.t1.exon2;Parent=MS.gene52572.t1 |
| chr7.4 | CDS | 26150703 | 26151110 | 26150703 | ID=cds.MS.gene52572.t1;Parent=MS.gene52572.t1 |
| Gene Sequence |
| Protein sequence |
