Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52575.t1 | XP_003624171.2 | 100 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 3.60E-53 | 217.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52575.t1 | Q9FFD8 | 77.4 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 4.1e-42 | 171.8 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52575.t1 | G7KRI0 | 100.0 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 2.6e-53 | 217.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene058726 | MS.gene52575 | 0.985415 | 1.70E-163 | -1.69E-46 |
| MS.gene058728 | MS.gene52575 | 0.9579 | 8.84E-116 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52575.t1 | MTR_7g079990 | 100.000 | 106 | 0 | 0 | 1 | 106 | 1 | 106 | 1.71e-73 | 213 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52575.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene52575.t1 | AT5G16470 | 77.358 | 106 | 22 | 1 | 1 | 106 | 1 | 104 | 8.61e-56 | 168 |
| MS.gene52575.t1 | AT3G02790 | 77.358 | 106 | 23 | 1 | 1 | 106 | 1 | 105 | 2.44e-55 | 167 |
Find 31 sgRNAs with CRISPR-Local
Find 35 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TCCTTCGCCGTGTGCTTCTT+TGG | 0.219018 | 7.4:+26127265 | None:intergenic |
| TCTTCCTCAAAAGGGATCTT+TGG | 0.247762 | 7.4:+26127073 | None:intergenic |
| CGTCAGCGCCGCGTCAACCT+TGG | 0.424775 | 7.4:+26127234 | None:intergenic |
| ATCTGCATCGATTTCACATC+AGG | 0.442800 | 7.4:+26127115 | None:intergenic |
| GCCAAAGAAGCACACGGCGA+AGG | 0.455639 | 7.4:-26127266 | MS.gene52575:CDS |
| GAAAAGCCGGTTTGCTTGAT+CGG | 0.458319 | 7.4:-26127198 | MS.gene52575:CDS |
| AAGCCTCGCCCTGGTGTTCG+TGG | 0.465707 | 7.4:-26127001 | MS.gene52575:CDS |
| ACGAACACCAGGGCGAGGCT+TGG | 0.472106 | 7.4:+26127003 | None:intergenic |
| TGATCGGATCGGTGTTGAAA+AGG | 0.486280 | 7.4:-26127182 | MS.gene52575:CDS |
| GCGCCGCGTCAACCTTGGCT+TGG | 0.491264 | 7.4:+26127239 | None:intergenic |
| CGAGGCTTGGAGGATTCAGC+AGG | 0.496573 | 7.4:+26127016 | None:intergenic |
| AACACCAGGGCGAGGCTTGG+AGG | 0.501344 | 7.4:+26127006 | None:intergenic |
| GAATCCTCCAAGCCTCGCCC+TGG | 0.508082 | 7.4:-26127010 | MS.gene52575:CDS |
| ACCGATCCGATCAAGCAAAC+CGG | 0.511341 | 7.4:+26127192 | None:intergenic |
| GCGCTGACGAATCGCGGCGG+AGG | 0.512793 | 7.4:-26127220 | MS.gene52575:CDS |
| GCAGTGAGGGCACTCCCACT+TGG | 0.515764 | 7.4:+26127150 | None:intergenic |
| TTGAGACTACCACGAACACC+AGG | 0.527196 | 7.4:+26126992 | None:intergenic |
| GACGCGGCGCTGACGAATCG+CGG | 0.534103 | 7.4:-26127226 | MS.gene52575:CDS |
| GATCGGATCGGTGTTGAAAA+GGG | 0.542506 | 7.4:-26127181 | MS.gene52575:CDS |
| CAATCATCAATCAAGATGAC+GGG | 0.549212 | 7.4:-26127298 | None:intergenic |
| AATCGCGGCGGAGGAAAAGC+CGG | 0.554621 | 7.4:-26127211 | MS.gene52575:CDS |
| GCCGGTTTGCTTGATCGGAT+CGG | 0.570802 | 7.4:-26127193 | MS.gene52575:CDS |
| CTACCACGAACACCAGGGCG+AGG | 0.597449 | 7.4:+26126998 | None:intergenic |
| CGGATCGGTGTTGAAAAGGG+TGG | 0.630433 | 7.4:-26127178 | MS.gene52575:CDS |
| GGCGAAGGAGATCCAAGCCA+AGG | 0.648739 | 7.4:-26127251 | MS.gene52575:CDS |
| AGCGAAGCCAAAGAAGCACA+CGG | 0.650014 | 7.4:-26127272 | MS.gene52575:CDS |
| GATCCAAGCCAAGGTTGACG+CGG | 0.654184 | 7.4:-26127242 | MS.gene52575:CDS |
| AAAAGGGTGGACACGCCAAG+TGG | 0.671036 | 7.4:-26127165 | MS.gene52575:CDS |
| GCGGCGCTGACGAATCGCGG+CGG | 0.674143 | 7.4:-26127223 | MS.gene52575:CDS |
| AAAGGGTGGACACGCCAAGT+GGG | 0.690189 | 7.4:-26127164 | MS.gene52575:CDS |
| TGAGACTACCACGAACACCA+GGG | 0.697182 | 7.4:+26126993 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| ! | ACAAGCTTTTCTTCCTCAAA+AGG | + | chr7.4:26127207-26127226 | None:intergenic | 35.0% |
| ! | CAAGCTTTTCTTCCTCAAAA+GGG | + | chr7.4:26127206-26127225 | None:intergenic | 35.0% |
| TCTTCCTCAAAAGGGATCTT+TGG | + | chr7.4:26127198-26127217 | None:intergenic | 40.0% | |
| !! | ATCTGCATCGATTTCACATC+AGG | + | chr7.4:26127156-26127175 | None:intergenic | 40.0% |
| ! | GCATCCAAAGATCCCTTTTG+AGG | - | chr7.4:26127191-26127210 | MS.gene52575:CDS | 45.0% |
| !! | GAAAAGCCGGTTTGCTTGAT+CGG | - | chr7.4:26127070-26127089 | MS.gene52575:CDS | 45.0% |
| !! | GATCGGATCGGTGTTGAAAA+GGG | - | chr7.4:26127087-26127106 | MS.gene52575:CDS | 45.0% |
| !! | TGATCGGATCGGTGTTGAAA+AGG | - | chr7.4:26127086-26127105 | MS.gene52575:CDS | 45.0% |
| ACCGATCCGATCAAGCAAAC+CGG | + | chr7.4:26127079-26127098 | None:intergenic | 50.0% | |
| TGAGACTACCACGAACACCA+GGG | + | chr7.4:26127278-26127297 | None:intergenic | 50.0% | |
| TTGAGACTACCACGAACACC+AGG | + | chr7.4:26127279-26127298 | None:intergenic | 50.0% | |
| ! | AGCGAAGCCAAAGAAGCACA+CGG | - | chr7.4:26126996-26127015 | MS.gene52575:CDS | 50.0% |
| ! | GCGCTGTGATTTTGCAGTGA+GGG | + | chr7.4:26127134-26127153 | None:intergenic | 50.0% |
| AAAAGGGTGGACACGCCAAG+TGG | - | chr7.4:26127103-26127122 | MS.gene52575:CDS | 55.0% | |
| AAAGGGTGGACACGCCAAGT+GGG | - | chr7.4:26127104-26127123 | MS.gene52575:CDS | 55.0% | |
| GATCCAAGCCAAGGTTGACG+CGG | - | chr7.4:26127026-26127045 | MS.gene52575:CDS | 55.0% | |
| TCCTTCGCCGTGTGCTTCTT+TGG | + | chr7.4:26127006-26127025 | None:intergenic | 55.0% | |
| ! | GGCGCTGTGATTTTGCAGTG+AGG | + | chr7.4:26127135-26127154 | None:intergenic | 55.0% |
| !! | CGGATCGGTGTTGAAAAGGG+TGG | - | chr7.4:26127090-26127109 | MS.gene52575:CDS | 55.0% |
| !! | GCCGGTTTGCTTGATCGGAT+CGG | - | chr7.4:26127075-26127094 | MS.gene52575:CDS | 55.0% |
| AATCGCGGCGGAGGAAAAGC+CGG | - | chr7.4:26127057-26127076 | MS.gene52575:CDS | 60.0% | |
| CGAGGCTTGGAGGATTCAGC+AGG | + | chr7.4:26127255-26127274 | None:intergenic | 60.0% | |
| GGCGAAGGAGATCCAAGCCA+AGG | - | chr7.4:26127017-26127036 | MS.gene52575:CDS | 60.0% | |
| ! | GCCAAAGAAGCACACGGCGA+AGG | - | chr7.4:26127002-26127021 | MS.gene52575:CDS | 60.0% |
| AACACCAGGGCGAGGCTTGG+AGG | + | chr7.4:26127265-26127284 | None:intergenic | 65.0% | |
| AAGCCTCGCCCTGGTGTTCG+TGG | - | chr7.4:26127267-26127286 | MS.gene52575:CDS | 65.0% | |
| ACGAACACCAGGGCGAGGCT+TGG | + | chr7.4:26127268-26127287 | None:intergenic | 65.0% | |
| CTACCACGAACACCAGGGCG+AGG | + | chr7.4:26127273-26127292 | None:intergenic | 65.0% | |
| GAATCCTCCAAGCCTCGCCC+TGG | - | chr7.4:26127258-26127277 | MS.gene52575:CDS | 65.0% | |
| !! | GCAGTGAGGGCACTCCCACT+TGG | + | chr7.4:26127121-26127140 | None:intergenic | 65.0% |
| CGTCAGCGCCGCGTCAACCT+TGG | + | chr7.4:26127037-26127056 | None:intergenic | 70.0% | |
| GCGCCGCGTCAACCTTGGCT+TGG | + | chr7.4:26127032-26127051 | None:intergenic | 70.0% | |
| !! | GACGCGGCGCTGACGAATCG+CGG | - | chr7.4:26127042-26127061 | MS.gene52575:CDS | 70.0% |
| ! | GCGCTGACGAATCGCGGCGG+AGG | - | chr7.4:26127048-26127067 | MS.gene52575:CDS | 75.0% |
| !! | GCGGCGCTGACGAATCGCGG+CGG | - | chr7.4:26127045-26127064 | MS.gene52575:CDS | 75.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.4 | gene | 26126985 | 26127305 | 26126985 | ID=MS.gene52575 |
| chr7.4 | mRNA | 26126985 | 26127305 | 26126985 | ID=MS.gene52575.t1;Parent=MS.gene52575 |
| chr7.4 | exon | 26126985 | 26127305 | 26126985 | ID=MS.gene52575.t1.exon1;Parent=MS.gene52575.t1 |
| chr7.4 | CDS | 26126985 | 26127305 | 26126985 | ID=cds.MS.gene52575.t1;Parent=MS.gene52575.t1 |
| Gene Sequence |
| Protein sequence |
