Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52806.t1 | XP_003603835.1 | 92.3 | 248 | 7 | 4 | 1 | 239 | 1 | 245 | 2.10E-109 | 405.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52806.t1 | Q9SGS5 | 39.4 | 277 | 107 | 10 | 6 | 238 | 13 | 272 | 2.6e-23 | 110.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52806.t1 | G7J5W8 | 92.3 | 248 | 7 | 4 | 1 | 239 | 1 | 245 | 1.5e-109 | 405.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050363 | MS.gene52806 | 0.85508 | 8.02E-62 | -1.69E-46 |
| MS.gene056311 | MS.gene52806 | 0.824225 | 9.06E-54 | -1.69E-46 |
| MS.gene056408 | MS.gene52806 | 0.801091 | 1.06E-48 | -1.69E-46 |
| MS.gene058494 | MS.gene52806 | 0.803412 | 3.53E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52806.t1 | MTR_3g114750 | 91.532 | 248 | 9 | 4 | 1 | 239 | 1 | 245 | 1.86e-147 | 411 |
| MS.gene52806.t1 | MTR_1g026110 | 49.035 | 259 | 91 | 9 | 2 | 238 | 4 | 243 | 3.93e-63 | 197 |
| MS.gene52806.t1 | MTR_5g023030 | 34.356 | 163 | 87 | 5 | 1 | 159 | 1 | 147 | 8.26e-12 | 61.6 |
| MS.gene52806.t1 | MTR_5g023030 | 34.356 | 163 | 87 | 5 | 1 | 159 | 1 | 147 | 2.48e-11 | 60.8 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52806.t1 | AT1G76070 | 34.686 | 271 | 112 | 12 | 14 | 238 | 21 | 272 | 2.02e-30 | 114 |
| MS.gene52806.t1 | AT1G20310 | 29.278 | 263 | 133 | 10 | 1 | 235 | 1 | 238 | 8.68e-20 | 85.5 |
Find 47 sgRNAs with CRISPR-Local
Find 57 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTCCTTCTTCCCTCAGATTT+TGG | 0.122137 | 3.1:-90832427 | None:intergenic |
| ATCAATTCCATTAATATCTT+TGG | 0.179497 | 3.1:-90832266 | None:intergenic |
| CTTGGTGGTGGTGGTGTTGC+TGG | 0.256841 | 3.1:+90832682 | MS.gene52806:CDS |
| CCAAGTTTGATCTAATTTAC+TGG | 0.265351 | 3.1:-90832796 | None:intergenic |
| AGAAAGTCCAAAGATATTAA+TGG | 0.282367 | 3.1:+90832259 | MS.gene52806:CDS |
| AGATCTTGTTCCTTAGCTTA+AGG | 0.361689 | 3.1:-90832099 | None:intergenic |
| GGTGTTGCTGGTTGTAGTCT+TGG | 0.383396 | 3.1:+90832694 | MS.gene52806:CDS |
| AAAAGATGTTATTTCATGTC+GGG | 0.387650 | 3.1:+90832401 | MS.gene52806:CDS |
| ATCCCTTTCTCTGCACCTCT+TGG | 0.403077 | 3.1:+90832664 | MS.gene52806:CDS |
| TCTAGCTTCATGTGGAATCA+TGG | 0.405796 | 3.1:-90832236 | None:intergenic |
| TCCACCTAGGCCTCTACAAC+TGG | 0.412969 | 3.1:+90832771 | MS.gene52806:CDS |
| CCTTTCTCTGCACCTCTTGG+TGG | 0.430613 | 3.1:+90832667 | MS.gene52806:CDS |
| TCTGCACCTCTTGGTGGTGG+TGG | 0.444151 | 3.1:+90832673 | MS.gene52806:CDS |
| CTTGATTTGTGATCCCTACC+TGG | 0.448791 | 3.1:-90832175 | None:intergenic |
| GACTTTCTTCTAGCTTCATG+TGG | 0.470996 | 3.1:-90832244 | None:intergenic |
| GGATCCAGTTGTAGAGGCCT+AGG | 0.476355 | 3.1:-90832775 | None:intergenic |
| TTCTCTGCACCTCTTGGTGG+TGG | 0.477822 | 3.1:+90832670 | MS.gene52806:CDS |
| TAGTAATTATCTATTTCCTC+TGG | 0.477991 | 3.1:-90832583 | None:intergenic |
| CACGTAAAGAAATTAATCTA+TGG | 0.479566 | 3.1:+90832731 | MS.gene52806:CDS |
| TGTGGAATCATGGATGCAAT+TGG | 0.480921 | 3.1:-90832226 | None:intergenic |
| CCACCAAGAGGTGCAGAGAA+AGG | 0.489526 | 3.1:-90832667 | None:intergenic |
| AACCAAAATCTGAGGGAAGA+AGG | 0.495894 | 3.1:+90832425 | MS.gene52806:CDS |
| TGATCCCTACCTGGACTGAA+TGG | 0.498481 | 3.1:-90832166 | None:intergenic |
| CACCAAGAGGTGCAGAGAAA+GGG | 0.499821 | 3.1:-90832666 | None:intergenic |
| CAAAAGATGTTATTTCATGT+CGG | 0.505412 | 3.1:+90832400 | MS.gene52806:CDS |
| AGATAAGAAGTATGCAATTG+AGG | 0.507400 | 3.1:+90832471 | MS.gene52806:CDS |
| CATAGATTAATTTCTTTACG+TGG | 0.529657 | 3.1:-90832730 | None:intergenic |
| ATCAAGACCTCATGGTGTCA+AGG | 0.530656 | 3.1:+90832192 | MS.gene52806:CDS |
| CAAAACCATCCATTCAGTCC+AGG | 0.540895 | 3.1:+90832157 | MS.gene52806:CDS |
| TCAAGACCTCATGGTGTCAA+GGG | 0.542315 | 3.1:+90832193 | MS.gene52806:CDS |
| TGATGATGATGAAGAAGAAG+AGG | 0.542344 | 3.1:+90832633 | MS.gene52806:CDS |
| CCCTACCTGGACTGAATGGA+TGG | 0.542832 | 3.1:-90832162 | None:intergenic |
| AAATTAATCTATGGAAGAGA+AGG | 0.544809 | 3.1:+90832740 | MS.gene52806:CDS |
| CCATCCATTCAGTCCAGGTA+GGG | 0.567822 | 3.1:+90832162 | MS.gene52806:CDS |
| TAGAGGCCTAGGTGGAGCCA+TGG | 0.570574 | 3.1:-90832764 | None:intergenic |
| TTTACTGGATCCAGTTGTAG+AGG | 0.574544 | 3.1:-90832781 | None:intergenic |
| GCAACACCACCACCACCAAG+AGG | 0.576743 | 3.1:-90832679 | None:intergenic |
| TGTCGGGAAACCAAAATCTG+AGG | 0.596722 | 3.1:+90832417 | MS.gene52806:CDS |
| GCATGGGACAAATCAAGCAC+AGG | 0.597529 | 3.1:+90832320 | MS.gene52806:CDS |
| GTCGGGAAACCAAAATCTGA+GGG | 0.603177 | 3.1:+90832418 | MS.gene52806:CDS |
| TCCAGTTGTAGAGGCCTAGG+TGG | 0.615336 | 3.1:-90832772 | None:intergenic |
| GATCACAAATCAAGACCTCA+TGG | 0.618438 | 3.1:+90832184 | MS.gene52806:CDS |
| GAAGGACCATGGCTCCACCT+AGG | 0.627085 | 3.1:+90832758 | MS.gene52806:CDS |
| GAAGGCTCAGGTAGCACCAG+AGG | 0.628886 | 3.1:+90832567 | MS.gene52806:CDS |
| GAAAATCCCTTGACACCATG+AGG | 0.634828 | 3.1:-90832199 | None:intergenic |
| ACCATCCATTCAGTCCAGGT+AGG | 0.635976 | 3.1:+90832161 | MS.gene52806:CDS |
| TCTATGGAAGAGAAGGACCA+TGG | 0.674583 | 3.1:+90832747 | MS.gene52806:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATCAATTCCATTAATATCTT+TGG | - | chr3.1:90832269-90832288 | None:intergenic | 20.0% |
| !!! | AAGTTTATAGTGATAGTTTT+TGG | - | chr3.1:90832136-90832155 | None:intergenic | 20.0% |
| ! | AAATTAATCTATGGAAGAGA+AGG | + | chr3.1:90832740-90832759 | MS.gene52806:CDS | 25.0% |
| ! | AGAAAGTCCAAAGATATTAA+TGG | + | chr3.1:90832259-90832278 | MS.gene52806:CDS | 25.0% |
| ! | CACGTAAAGAAATTAATCTA+TGG | + | chr3.1:90832731-90832750 | MS.gene52806:CDS | 25.0% |
| ! | CATAGATTAATTTCTTTACG+TGG | - | chr3.1:90832733-90832752 | None:intergenic | 25.0% |
| ! | TAGTAATTATCTATTTCCTC+TGG | - | chr3.1:90832586-90832605 | None:intergenic | 25.0% |
| !!! | AAAAGATGTTATTTCATGTC+GGG | + | chr3.1:90832401-90832420 | MS.gene52806:CDS | 25.0% |
| !!! | ACACTTTTGCTAATTTTGAT+TGG | + | chr3.1:90832545-90832564 | MS.gene52806:CDS | 25.0% |
| !!! | CAAAAGATGTTATTTCATGT+CGG | + | chr3.1:90832400-90832419 | MS.gene52806:CDS | 25.0% |
| !!! | TTTTGCTAATTTTGATTGGA+AGG | + | chr3.1:90832549-90832568 | MS.gene52806:CDS | 25.0% |
| ! | AGATAAGAAGTATGCAATTG+AGG | + | chr3.1:90832471-90832490 | MS.gene52806:CDS | 30.0% |
| AGATCTTGTTCCTTAGCTTA+AGG | - | chr3.1:90832102-90832121 | None:intergenic | 35.0% | |
| TCACCAAAAATATCGTGCAT+GGG | + | chr3.1:90832304-90832323 | MS.gene52806:CDS | 35.0% | |
| TGGAGAAAAACCTTAAGCTA+AGG | + | chr3.1:90832089-90832108 | MS.gene52806:CDS | 35.0% | |
| TTCACCAAAAATATCGTGCA+TGG | + | chr3.1:90832303-90832322 | MS.gene52806:CDS | 35.0% | |
| ! | CAAATGAAACGTTTTGCAAG+TGG | + | chr3.1:90832517-90832536 | MS.gene52806:CDS | 35.0% |
| ! | TGATGATGATGAAGAAGAAG+AGG | + | chr3.1:90832633-90832652 | MS.gene52806:CDS | 35.0% |
| !!! | CACGATATTTTTGGTGAAGT+TGG | - | chr3.1:90832301-90832320 | None:intergenic | 35.0% |
| !!! | TAATTTTGATTGGAAGGCTC+AGG | + | chr3.1:90832555-90832574 | MS.gene52806:CDS | 35.0% |
| AACCAAAATCTGAGGGAAGA+AGG | + | chr3.1:90832425-90832444 | MS.gene52806:CDS | 40.0% | |
| GATCACAAATCAAGACCTCA+TGG | + | chr3.1:90832184-90832203 | MS.gene52806:CDS | 40.0% | |
| TGTGGAATCATGGATGCAAT+TGG | - | chr3.1:90832229-90832248 | None:intergenic | 40.0% | |
| TTTACTGGATCCAGTTGTAG+AGG | - | chr3.1:90832784-90832803 | None:intergenic | 40.0% | |
| ! | TCTAGCTTCATGTGGAATCA+TGG | - | chr3.1:90832239-90832258 | None:intergenic | 40.0% |
| ! | TGTCCCATGCACGATATTTT+TGG | - | chr3.1:90832310-90832329 | None:intergenic | 40.0% |
| ! | TTCCTTCTTCCCTCAGATTT+TGG | - | chr3.1:90832430-90832449 | None:intergenic | 40.0% |
| !! | GACTTTCTTCTAGCTTCATG+TGG | - | chr3.1:90832247-90832266 | None:intergenic | 40.0% |
| CAAAACCATCCATTCAGTCC+AGG | + | chr3.1:90832157-90832176 | MS.gene52806:CDS | 45.0% | |
| GAAAATCCCTTGACACCATG+AGG | - | chr3.1:90832202-90832221 | None:intergenic | 45.0% | |
| GTCGGGAAACCAAAATCTGA+GGG | + | chr3.1:90832418-90832437 | MS.gene52806:CDS | 45.0% | |
| TCTATGGAAGAGAAGGACCA+TGG | + | chr3.1:90832747-90832766 | MS.gene52806:CDS | 45.0% | |
| TGTCGGGAAACCAAAATCTG+AGG | + | chr3.1:90832417-90832436 | MS.gene52806:CDS | 45.0% | |
| ! | ATCAAGACCTCATGGTGTCA+AGG | + | chr3.1:90832192-90832211 | MS.gene52806:CDS | 45.0% |
| ! | TCAAGACCTCATGGTGTCAA+GGG | + | chr3.1:90832193-90832212 | MS.gene52806:CDS | 45.0% |
| !! | CATGGTGTCAAGGGATTTTC+AGG | + | chr3.1:90832202-90832221 | MS.gene52806:CDS | 45.0% |
| !! | CTTGATTTGTGATCCCTACC+TGG | - | chr3.1:90832178-90832197 | None:intergenic | 45.0% |
| ACCATCCATTCAGTCCAGGT+AGG | + | chr3.1:90832161-90832180 | MS.gene52806:CDS | 50.0% | |
| ATCCCTTTCTCTGCACCTCT+TGG | + | chr3.1:90832664-90832683 | MS.gene52806:CDS | 50.0% | |
| CCATCCATTCAGTCCAGGTA+GGG | + | chr3.1:90832162-90832181 | MS.gene52806:CDS | 50.0% | |
| GCATGGGACAAATCAAGCAC+AGG | + | chr3.1:90832320-90832339 | MS.gene52806:CDS | 50.0% | |
| TGATCCCTACCTGGACTGAA+TGG | - | chr3.1:90832169-90832188 | None:intergenic | 50.0% | |
| ! | CACCAAGAGGTGCAGAGAAA+GGG | - | chr3.1:90832669-90832688 | None:intergenic | 50.0% |
| !! | GGTGTTGCTGGTTGTAGTCT+TGG | + | chr3.1:90832694-90832713 | MS.gene52806:CDS | 50.0% |
| CCCTACCTGGACTGAATGGA+TGG | - | chr3.1:90832165-90832184 | None:intergenic | 55.0% | |
| CCTTTCTCTGCACCTCTTGG+TGG | + | chr3.1:90832667-90832686 | MS.gene52806:CDS | 55.0% | |
| GGATCCAGTTGTAGAGGCCT+AGG | - | chr3.1:90832778-90832797 | None:intergenic | 55.0% | |
| TCCACCTAGGCCTCTACAAC+TGG | + | chr3.1:90832771-90832790 | MS.gene52806:CDS | 55.0% | |
| TCCAGTTGTAGAGGCCTAGG+TGG | - | chr3.1:90832775-90832794 | None:intergenic | 55.0% | |
| TTCTCTGCACCTCTTGGTGG+TGG | + | chr3.1:90832670-90832689 | MS.gene52806:CDS | 55.0% | |
| ! | CCACCAAGAGGTGCAGAGAA+AGG | - | chr3.1:90832670-90832689 | None:intergenic | 55.0% |
| GAAGGACCATGGCTCCACCT+AGG | + | chr3.1:90832758-90832777 | MS.gene52806:CDS | 60.0% | |
| GAAGGCTCAGGTAGCACCAG+AGG | + | chr3.1:90832567-90832586 | MS.gene52806:CDS | 60.0% | |
| GCAACACCACCACCACCAAG+AGG | - | chr3.1:90832682-90832701 | None:intergenic | 60.0% | |
| TAGAGGCCTAGGTGGAGCCA+TGG | - | chr3.1:90832767-90832786 | None:intergenic | 60.0% | |
| !! | CTTGGTGGTGGTGGTGTTGC+TGG | + | chr3.1:90832682-90832701 | MS.gene52806:CDS | 60.0% |
| !! | TCTGCACCTCTTGGTGGTGG+TGG | + | chr3.1:90832673-90832692 | MS.gene52806:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 90832088 | 90832807 | 90832088 | ID=MS.gene52806 |
| chr3.1 | mRNA | 90832088 | 90832807 | 90832088 | ID=MS.gene52806.t1;Parent=MS.gene52806 |
| chr3.1 | exon | 90832088 | 90832807 | 90832088 | ID=MS.gene52806.t1.exon1;Parent=MS.gene52806.t1 |
| chr3.1 | CDS | 90832088 | 90832807 | 90832088 | ID=cds.MS.gene52806.t1;Parent=MS.gene52806.t1 |
| Gene Sequence |
| Protein sequence |
