Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52836.t1 | XP_013468649.1 | 98.3 | 238 | 4 | 0 | 1 | 238 | 623 | 860 | 4.40E-136 | 493.8 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52836.t1 | O53732 | 39.1 | 225 | 134 | 2 | 1 | 225 | 204 | 425 | 1.5e-39 | 164.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52836.t1 | A0A072VM29 | 98.3 | 238 | 4 | 0 | 1 | 238 | 623 | 860 | 3.2e-136 | 493.8 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene049758 | MS.gene52836 | 0.80085 | 1.19E-48 | -1.69E-46 |
| MS.gene052078 | MS.gene52836 | 0.8115 | 6.79E-51 | -1.69E-46 |
| MS.gene05811 | MS.gene52836 | 0.800031 | 1.74E-48 | -1.69E-46 |
| MS.gene05984 | MS.gene52836 | 0.811287 | 7.55E-51 | -1.69E-46 |
| MS.gene061049 | MS.gene52836 | 0.805295 | 1.43E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52836.t1 | MTR_1g073700 | 98.319 | 238 | 4 | 0 | 1 | 238 | 623 | 860 | 4.76e-171 | 493 |
| MS.gene52836.t1 | MTR_1g044520 | 81.034 | 232 | 44 | 0 | 1 | 232 | 587 | 818 | 1.52e-137 | 406 |
| MS.gene52836.t1 | MTR_3g104740 | 27.807 | 187 | 128 | 5 | 1 | 185 | 128 | 309 | 1.55e-15 | 75.1 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene52836.t1 | AT3G23510 | 75.630 | 238 | 58 | 0 | 1 | 238 | 624 | 861 | 2.37e-135 | 401 |
| MS.gene52836.t1 | AT3G23530 | 76.050 | 238 | 57 | 0 | 1 | 238 | 624 | 861 | 2.71e-134 | 399 |
| MS.gene52836.t1 | AT3G23470 | 70.588 | 238 | 68 | 1 | 1 | 236 | 216 | 453 | 4.00e-128 | 370 |
| MS.gene52836.t1 | AT3G23470 | 70.588 | 238 | 68 | 1 | 1 | 236 | 243 | 480 | 2.10e-127 | 369 |
| MS.gene52836.t1 | AT3G23470 | 70.588 | 238 | 68 | 1 | 1 | 236 | 243 | 480 | 2.10e-127 | 369 |
| MS.gene52836.t1 | AT3G23480 | 71.064 | 235 | 66 | 1 | 4 | 236 | 159 | 393 | 2.63e-124 | 360 |
| MS.gene52836.t1 | AT3G23480 | 71.064 | 235 | 66 | 1 | 4 | 236 | 196 | 430 | 4.91e-124 | 361 |
| MS.gene52836.t1 | AT3G23480 | 69.333 | 225 | 69 | 0 | 4 | 228 | 217 | 441 | 5.59e-117 | 341 |
| MS.gene52836.t1 | AT3G23460 | 72.368 | 76 | 21 | 0 | 157 | 232 | 209 | 284 | 3.44e-36 | 130 |
| MS.gene52836.t1 | AT4G33110 | 30.526 | 190 | 122 | 6 | 1 | 188 | 125 | 306 | 3.09e-17 | 80.1 |
| MS.gene52836.t1 | AT4G33110 | 30.526 | 190 | 122 | 6 | 1 | 188 | 125 | 306 | 3.09e-17 | 80.1 |
| MS.gene52836.t1 | AT4G33120 | 27.885 | 208 | 138 | 7 | 1 | 202 | 125 | 326 | 4.10e-17 | 79.7 |
Find 0 sgRNAs with CRISPR-Local
Find 80 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTTTTATCAATAAAAGTTT+AGG | + | 11198:1038-1057 | MS.gene52836:intron | 10.0% |
| !! | ATAAAAATAGAAGAATTCAA+CGG | - | 11198:1026-1045 | None:intergenic | 15.0% |
| !! | TTTGATCTAGATTTATAATA+TGG | - | 11198:1460-1479 | None:intergenic | 15.0% |
| !! | AAATCATACAAATATAACCA+AGG | - | 11198:727-746 | None:intergenic | 20.0% |
| !! | AATATGATAGGATTATATCT+TGG | + | 11198:482-501 | MS.gene52836:CDS | 20.0% |
| !!! | ATAAAGGAATATATTTTTCC+AGG | + | 11198:920-939 | MS.gene52836:CDS | 20.0% |
| !!! | GAATACTATTTTGATTATTG+TGG | + | 11198:1385-1404 | MS.gene52836:CDS | 20.0% |
| ! | AAACATGAAATTCTTGAGAT+TGG | + | 11198:91-110 | MS.gene52836:CDS | 25.0% |
| ! | AAATGTTACAGTTAAAGTCT+TGG | - | 11198:1092-1111 | None:intergenic | 25.0% |
| ! | ATACAAGTTATGTACTATGT+TGG | - | 11198:562-581 | None:intergenic | 25.0% |
| ! | ATGAAAAATTCATCAGAACA+TGG | + | 11198:1362-1381 | MS.gene52836:CDS | 25.0% |
| ! | ATTGAAGTTGTTAAAAGAAC+TGG | + | 11198:136-155 | MS.gene52836:CDS | 25.0% |
| ! | ATTGTGGAGAAAAAATTTCA+TGG | + | 11198:1205-1224 | MS.gene52836:CDS | 25.0% |
| ! | CAAAGACATACAAATATGAT+AGG | + | 11198:470-489 | MS.gene52836:CDS | 25.0% |
| ! | CATTAAAGATACATAGTTGT+TGG | + | 11198:798-817 | MS.gene52836:intron | 25.0% |
| ! | CTATCATATTTGTATGTCTT+TGG | - | 11198:472-491 | None:intergenic | 25.0% |
| ! | CTTTGTTAATTAATACCTGA+AGG | - | 11198:234-253 | None:intergenic | 25.0% |
| ! | TGAAAAATTCATCAGAACAT+GGG | + | 11198:1363-1382 | MS.gene52836:CDS | 25.0% |
| ! | TGTACTATGTTGGAAATATA+TGG | - | 11198:552-571 | None:intergenic | 25.0% |
| !! | AGTTCTTTTAACAACTTCAA+TGG | - | 11198:137-156 | None:intergenic | 25.0% |
| !!! | AGCATCTTTAACTCTTTTTT+CGG | - | 11198:209-228 | None:intergenic | 25.0% |
| ACTATCAAACACTAAGATTG+TGG | + | 11198:1189-1208 | MS.gene52836:CDS | 30.0% | |
| CACAGTAACAAAGATTATAG+AGG | - | 11198:359-378 | None:intergenic | 30.0% | |
| GAAAAAAGAGTTAAAGATGC+TGG | + | 11198:208-227 | MS.gene52836:CDS | 30.0% | |
| GAATACATGGAAGAATTCTT+TGG | + | 11198:620-639 | MS.gene52836:CDS | 30.0% | |
| GCGAATATTCAGTTAAGTAA+GGG | - | 11198:1129-1148 | None:intergenic | 30.0% | |
| GGAGAAAAAATTTCATGGAA+AGG | + | 11198:1210-1229 | MS.gene52836:CDS | 30.0% | |
| TAGATTATAAGCTTTCTGCA+AGG | + | 11198:759-778 | MS.gene52836:intron | 30.0% | |
| TATTAGATTGTACCATAGTC+TGG | - | 11198:996-1015 | None:intergenic | 30.0% | |
| TCTATAGTTCATATCAATCC+CGG | + | 11198:859-878 | MS.gene52836:intron | 30.0% | |
| TGCGAATATTCAGTTAAGTA+AGG | - | 11198:1130-1149 | None:intergenic | 30.0% | |
| TTCTTGATGTTATCAAACCA+TGG | + | 11198:401-420 | MS.gene52836:intron | 30.0% | |
| TTTGTGCTAACTATTGAAGA+TGG | + | 11198:829-848 | MS.gene52836:intron | 30.0% | |
| ! | AAGGAATATATTTTTCCAGG+AGG | + | 11198:923-942 | MS.gene52836:CDS | 30.0% |
| ! | ATCAAACTTTTGCTTTACCT+TGG | + | 11198:707-726 | MS.gene52836:intron | 30.0% |
| ! | TTGTGTTCTCAGTGAAATTT+TGG | + | 11198:1327-1346 | MS.gene52836:intron | 30.0% |
| !!! | TATTTTGATTATTGTGGTGC+TGG | + | 11198:1391-1410 | MS.gene52836:CDS | 30.0% |
| AAAATTTCATGGAAAGGCAG+AGG | + | 11198:1216-1235 | MS.gene52836:CDS | 35.0% | |
| AGTGAAATGATAGAAGCTGT+TGG | + | 11198:593-612 | MS.gene52836:intron | 35.0% | |
| ATATGTTTCATGTGGCATGT+AGG | + | 11198:1514-1533 | MS.gene52836:intron | 35.0% | |
| ATGAGCGTTATGATGAGTAT+AGG | + | 11198:882-901 | MS.gene52836:CDS | 35.0% | |
| CAACAGTGATATGTTTCATG+TGG | + | 11198:1506-1525 | MS.gene52836:intron | 35.0% | |
| GAAATTCTTGAGATTGGATG+TGG | + | 11198:97-116 | MS.gene52836:CDS | 35.0% | |
| GAATCAGTAATGGCAAATGA+TGG | + | 11198:650-669 | MS.gene52836:CDS | 35.0% | |
| TGGTTCTTGTGAATCAGTAA+TGG | + | 11198:640-659 | MS.gene52836:CDS | 35.0% | |
| TGTATGTCTTTGGCAATTGT+CGG | - | 11198:462-481 | None:intergenic | 35.0% | |
| TGTTATCAAACCATGGTTAC+AGG | + | 11198:408-427 | MS.gene52836:intron | 35.0% | |
| TTGATATTGTCCTGTAACCA+TGG | - | 11198:421-440 | None:intergenic | 35.0% | |
| !! | GCGCAGTTCTGATTTTATAA+AGG | + | 11198:904-923 | MS.gene52836:CDS | 35.0% |
| AAGTAGGATCACATCATCCA+TGG | + | 11198:961-980 | MS.gene52836:CDS | 40.0% | |
| AGAACTGGTTGCAAATACAC+CGG | + | 11198:151-170 | MS.gene52836:CDS | 40.0% | |
| AGCTGTTGGACATGAATACA+TGG | + | 11198:607-626 | MS.gene52836:CDS | 40.0% | |
| ATTGTACCATAGTCTGGATG+CGG | - | 11198:990-1009 | None:intergenic | 40.0% | |
| GATGTGATCCTACTTAGAGA+GGG | - | 11198:956-975 | None:intergenic | 40.0% | |
| TACTCATCATAACGCTCATC+CGG | - | 11198:881-900 | None:intergenic | 40.0% | |
| TCAGTGATCCATACAAAAGC+TGG | + | 11198:1570-1589 | MS.gene52836:CDS | 40.0% | |
| TCTTGAGATTGGATGTGGAT+GGG | + | 11198:102-121 | MS.gene52836:CDS | 40.0% | |
| TGATGTGATCCTACTTAGAG+AGG | - | 11198:957-976 | None:intergenic | 40.0% | |
| TTCTTGAGATTGGATGTGGA+TGG | + | 11198:101-120 | MS.gene52836:CDS | 40.0% | |
| !! | GTTTTAAGTCACGCACACTT+GGG | + | 11198:1413-1432 | MS.gene52836:CDS | 40.0% |
| !! | TGATGGACTTCTTGTTCTTC+AGG | + | 11198:667-686 | MS.gene52836:CDS | 40.0% |
| !!! | CTCAGTGAAATTTTGGCTCT+AGG | + | 11198:1334-1353 | MS.gene52836:intron | 40.0% |
| ACGCACACTTGGGAATTATC+AGG | + | 11198:1423-1442 | MS.gene52836:CDS | 45.0% | |
| ACTCATCATAACGCTCATCC+GGG | - | 11198:880-899 | None:intergenic | 45.0% | |
| CTCCTCTGACAAAGTGATAC+CGG | - | 11198:173-192 | None:intergenic | 45.0% | |
| CTTGAGATTGGATGTGGATG+GGG | + | 11198:103-122 | MS.gene52836:CDS | 45.0% | |
| GTCGAGCACGTTGAAAACTT+CGG | + | 11198:1158-1177 | MS.gene52836:CDS | 45.0% | |
| TGAATGCAGTAACGTTCCCA+GGG | - | 11198:1553-1572 | None:intergenic | 45.0% | |
| TGATCCTACTTAGAGAGGGT+AGG | - | 11198:952-971 | None:intergenic | 45.0% | |
| TGTTTCATGTGGCATGTAGG+TGG | + | 11198:1517-1536 | MS.gene52836:intron | 45.0% | |
| !! | AGTTAAAGATGCTGGCCTTC+AGG | + | 11198:216-235 | MS.gene52836:CDS | 45.0% |
| !!! | GGTTTTAAGTCACGCACACT+TGG | + | 11198:1412-1431 | MS.gene52836:CDS | 45.0% |
| CACCGGTATCACTTTGTCAG+AGG | + | 11198:168-187 | MS.gene52836:CDS | 50.0% | |
| CTGAATGCAGTAACGTTCCC+AGG | - | 11198:1554-1573 | None:intergenic | 50.0% | |
| GTTGCCTACCCTCTCTAAGT+AGG | + | 11198:945-964 | MS.gene52836:CDS | 50.0% | |
| TAGTCTGGATGCGGATGCCA+TGG | - | 11198:981-1000 | None:intergenic | 55.0% | |
| TGGCATCCGCATCCAGACTA+TGG | + | 11198:981-1000 | MS.gene52836:CDS | 55.0% | |
| ! | AGGTGGTTTTCTCACGCCCT+GGG | + | 11198:1534-1553 | MS.gene52836:intron | 55.0% |
| ! | TAGGTGGTTTTCTCACGCCC+TGG | + | 11198:1533-1552 | MS.gene52836:intron | 55.0% |
| AGAGAGGGTAGGCAACCTCC+TGG | - | 11198:941-960 | None:intergenic | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| 11198 | gene | 76 | 1598 | 76 | ID=MS.gene52836 |
| 11198 | mRNA | 76 | 1598 | 76 | ID=MS.gene52836.t1;Parent=MS.gene52836 |
| 11198 | exon | 76 | 237 | 76 | ID=MS.gene52836.t1.exon1;Parent=MS.gene52836.t1 |
| 11198 | CDS | 76 | 237 | 76 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 430 | 503 | 430 | ID=MS.gene52836.t1.exon2;Parent=MS.gene52836.t1 |
| 11198 | CDS | 430 | 503 | 430 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 595 | 688 | 595 | ID=MS.gene52836.t1.exon3;Parent=MS.gene52836.t1 |
| 11198 | CDS | 595 | 688 | 595 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 866 | 1002 | 866 | ID=MS.gene52836.t1.exon4;Parent=MS.gene52836.t1 |
| 11198 | CDS | 866 | 1002 | 866 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 1157 | 1237 | 1157 | ID=MS.gene52836.t1.exon5;Parent=MS.gene52836.t1 |
| 11198 | CDS | 1157 | 1237 | 1157 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 1339 | 1444 | 1339 | ID=MS.gene52836.t1.exon6;Parent=MS.gene52836.t1 |
| 11198 | CDS | 1339 | 1444 | 1339 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| 11198 | exon | 1536 | 1598 | 1536 | ID=MS.gene52836.t1.exon7;Parent=MS.gene52836.t1 |
| 11198 | CDS | 1536 | 1598 | 1536 | ID=cds.MS.gene52836.t1;Parent=MS.gene52836.t1 |
| Gene Sequence |
| Protein sequence |
