AlfalfaGEDB | MS.gene53839

M. sativa cultivar XinJiangDaYe / MS.gene53839

KEGG: -
GO: -
PFAM: PF12819.10

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53839.t1	RHN44465.1	88.3	163	19	0	1	163	238	400	2.80E-81	311.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53839.t1	Q9SCZ4	43.4	159	86	3	2	158	226	382	5.6e-30	132.1

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53839.t1	A0A396H030	88.3	163	19	0	1	163	238	400	2.0e-81	311.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene051544	MS.gene53839	0.817734	2.83E-52	-1.69E-46
MS.gene058426	MS.gene53839	0.837205	5.96E-57	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53839.t1	MTR_7g015620	87.117	163	21	0	1	163	234	396	3.74e-99	300
MS.gene53839.t1	MTR_7g015420	85.276	163	24	0	1	163	234	396	8.02e-95	297
MS.gene53839.t1	MTR_7g015230	76.543	162	37	1	2	163	245	405	7.11e-85	266
MS.gene53839.t1	MTR_7g015310	75.460	163	39	1	2	163	239	401	2.85e-83	262
MS.gene53839.t1	MTR_7g015280	75.926	162	38	1	2	163	231	391	4.99e-83	261
MS.gene53839.t1	MTR_7g015320	73.837	172	35	2	1	163	239	409	1.01e-82	261
MS.gene53839.t1	MTR_4g052290	74.691	162	40	1	2	163	238	398	2.28e-81	257
MS.gene53839.t1	MTR_7g015670	74.074	162	41	1	2	163	241	401	3.31e-80	254
MS.gene53839.t1	MTR_7g015390	71.605	162	45	1	2	163	242	402	3.84e-76	243
MS.gene53839.t1	MTR_7g015510	72.222	162	44	1	2	163	242	402	1.31e-75	242
MS.gene53839.t1	MTR_7g015250	66.286	175	46	3	1	163	233	406	1.89e-73	236
MS.gene53839.t1	MTR_7g015550	70.988	162	46	1	2	163	242	402	9.95e-73	234
MS.gene53839.t1	MTR_7g015240	71.429	161	43	2	1	161	237	394	2.19e-72	233
MS.gene53839.t1	MTR_7g073660	42.683	164	76	6	2	154	226	382	5.36e-31	117
MS.gene53839.t1	MTR_4g111925	42.683	164	76	6	2	154	226	382	5.99e-31	117
MS.gene53839.t1	MTR_6g015805	42.000	150	79	6	3	147	225	371	6.35e-27	105
MS.gene53839.t1	MTR_5g047060	40.909	154	74	5	2	147	230	374	1.31e-26	105
MS.gene53839.t1	MTR_6g015795	38.667	150	84	5	3	147	231	377	7.53e-26	102
MS.gene53839.t1	MTR_5g047110	40.645	155	72	6	2	147	230	373	1.33e-25	102
MS.gene53839.t1	MTR_1g080740	34.591	159	97	4	2	158	215	368	2.53e-24	98.6
MS.gene53839.t1	MTR_7g115300	36.478	159	90	6	2	157	225	375	9.03e-23	94.4
MS.gene53839.t1	MTR_5g047120	32.000	150	94	3	3	147	238	384	6.49e-19	83.2
MS.gene53839.t1	MTR_5g047070	38.710	93	54	1	58	147	51	143	1.16e-17	79.3
MS.gene53839.t1	MTR_2g030310	32.432	148	88	5	2	145	224	363	2.21e-16	75.9
MS.gene53839.t1	MTR_2g030310	32.432	148	88	5	2	145	224	363	2.24e-16	75.9
MS.gene53839.t1	MTR_8g037700	30.864	162	103	5	2	158	242	399	3.34e-14	69.7
MS.gene53839.t1	MTR_2g080220	32.468	154	96	5	2	153	211	358	9.02e-14	68.2
MS.gene53839.t1	MTR_8g467150	31.343	134	76	5	2	126	209	335	2.08e-11	61.6
MS.gene53839.t1	MTR_2g096160	27.742	155	106	4	2	153	213	364	2.92e-11	61.2
MS.gene53839.t1	MTR_4g061833	31.579	133	79	5	2	130	64	188	8.37e-11	59.7

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene53839.t1	AT3G51550	42.138	159	88	2	2	158	226	382	2.17e-34	127
MS.gene53839.t1	AT5G39000	41.772	158	80	5	2	154	223	373	5.27e-28	109
MS.gene53839.t1	AT3G04690	37.423	163	91	6	2	158	213	370	9.45e-27	105
MS.gene53839.t1	AT5G38990	41.875	160	85	5	2	157	217	372	1.06e-26	105
MS.gene53839.t1	AT5G39020	39.610	154	86	4	2	154	220	367	1.51e-26	105
MS.gene53839.t1	AT5G28680	38.365	159	87	5	2	154	214	367	2.32e-26	104
MS.gene53839.t1	AT5G28680	38.365	159	87	5	2	154	214	367	2.32e-26	104
MS.gene53839.t1	AT5G39030	38.365	159	83	6	2	154	219	368	4.18e-25	100
MS.gene53839.t1	AT5G39030	38.365	159	83	6	2	154	213	362	4.52e-25	100
MS.gene53839.t1	AT1G30570	33.758	157	90	6	2	153	228	375	8.40e-21	88.6
MS.gene53839.t1	AT5G59700	36.585	123	70	4	2	122	202	318	3.06e-14	69.7
MS.gene53839.t1	AT5G54380	33.544	158	92	7	2	157	211	357	1.24e-13	67.8
MS.gene53839.t1	AT4G39110	33.784	148	90	5	2	147	240	381	4.09e-13	66.6
MS.gene53839.t1	AT4G00300	33.557	149	90	7	1	147	214	355	6.50e-13	65.9
MS.gene53839.t1	AT2G21480	35.135	148	88	6	2	147	239	380	2.20e-12	64.3
MS.gene53839.t1	AT3G46290	31.579	133	79	5	2	130	203	327	3.30e-11	60.8

Find 30 sgRNAs with CRISPR-Local

Find 31 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATATTTATCGATGATATTT+TGG	0.161759	7.4:-84024777	MS.gene53839:CDS
CTTAGTGTATCAAGTGTTTA+TGG	0.192099	7.4:-84025013	MS.gene53839:CDS
ATACAGGTATGTTTCGTAAT+TGG	0.195211	7.4:-84025075	MS.gene53839:CDS
ACTTCCGGTGCAGTGTAATT+TGG	0.202010	7.4:+84024956	None:intergenic
GTGAAAATGTACTCTCAAAA+TGG	0.301581	7.4:-84024686	MS.gene53839:CDS
CAATGATTATCCTGGTTACT+TGG	0.345911	7.4:-84025050	MS.gene53839:CDS
TACAGGTATGTTTCGTAATT+GGG	0.359203	7.4:-84025074	MS.gene53839:CDS
TACTTTACTGCAAGAAGTTA+TGG	0.359689	7.4:-84024929	MS.gene53839:CDS
GATCCGCTTATCAAGAATAA+AGG	0.374070	7.4:-84024815	MS.gene53839:CDS
TAACATGATTCCTGTTCATA+AGG	0.412064	7.4:-84024717	MS.gene53839:CDS
GCAGTAAAGTATACAACTTC+CGG	0.414772	7.4:+84024941	None:intergenic
CACCTTATCACATCGGCATC+TGG	0.417815	7.4:+84024749	None:intergenic
ACAGCATAATCCTTATGAAC+AGG	0.433445	7.4:+84024707	None:intergenic
CAGAGGATTATAATGTAACT+TGG	0.462446	7.4:-84024895	MS.gene53839:CDS
AATTGGGACAATGATTATCC+TGG	0.511473	7.4:-84025058	MS.gene53839:CDS
AACCAGATGCCGATGTGATA+AGG	0.515610	7.4:-84024751	MS.gene53839:CDS
CGGTGCAGTGTAATTTGGAA+TGG	0.521014	7.4:+84024961	None:intergenic
GTTGAACCAAGTGATGATAC+AGG	0.538055	7.4:-84025091	MS.gene53839:CDS
TCACCTTTATTCTTGATAAG+CGG	0.547794	7.4:+84024812	None:intergenic
TAAACACTTGATACACTAAG+TGG	0.552109	7.4:+84025016	None:intergenic
TATTGTTTCTCCAAGTAACC+AGG	0.569783	7.4:+84025040	None:intergenic
AACATACCTGTATCATCACT+TGG	0.571312	7.4:+84025085	None:intergenic
CATTCCAAATTACACTGCAC+CGG	0.592742	7.4:-84024960	MS.gene53839:CDS
CAGATGCCGATGTGATAAGG+TGG	0.633194	7.4:-84024748	MS.gene53839:CDS
ACCACTCCACCTTATCACAT+CGG	0.660024	7.4:+84024742	None:intergenic
ATGGTTTATAGAGTCAACGT+TGG	0.674503	7.4:-84025124	MS.gene53839:CDS
GCCGATGTGATAAGGTGGAG+TGG	0.694565	7.4:-84024743	MS.gene53839:CDS
AGTTACATTATAATCCTCTG+TGG	0.712355	7.4:+84024898	None:intergenic
GATGTGATAAGGTGGAGTGG+TGG	0.740563	7.4:-84024740	MS.gene53839:CDS
TTATGGAATGAATGCCACAG+AGG	0.742727	7.4:-84024912	MS.gene53839:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!!	CTTCTTTTACTTATATGATA+AGG	-	chr7.4:84024923-84024942	MS.gene53839:CDS	20.0%
!!!	GATATTTATCGATGATATTT+TGG	-	chr7.4:84025002-84025021	MS.gene53839:CDS	20.0%
	AGTTACATTATAATCCTCTG+TGG	+	chr7.4:84024884-84024903	None:intergenic	30.0%
	ATACAGGTATGTTTCGTAAT+TGG	-	chr7.4:84024704-84024723	MS.gene53839:CDS	30.0%
	CAGAGGATTATAATGTAACT+TGG	-	chr7.4:84024884-84024903	MS.gene53839:CDS	30.0%
	CTTAGTGTATCAAGTGTTTA+TGG	-	chr7.4:84024766-84024785	MS.gene53839:CDS	30.0%
	GTGAAAATGTACTCTCAAAA+TGG	-	chr7.4:84025093-84025112	MS.gene53839:CDS	30.0%
	TAAACACTTGATACACTAAG+TGG	+	chr7.4:84024766-84024785	None:intergenic	30.0%
	TAACATGATTCCTGTTCATA+AGG	-	chr7.4:84025062-84025081	MS.gene53839:CDS	30.0%
	TACAGGTATGTTTCGTAATT+GGG	-	chr7.4:84024705-84024724	MS.gene53839:CDS	30.0%
	TACTTTACTGCAAGAAGTTA+TGG	-	chr7.4:84024850-84024869	MS.gene53839:CDS	30.0%
	TCACCTTTATTCTTGATAAG+CGG	+	chr7.4:84024970-84024989	None:intergenic	30.0%
	AACATACCTGTATCATCACT+TGG	+	chr7.4:84024697-84024716	None:intergenic	35.0%
	AATTGGGACAATGATTATCC+TGG	-	chr7.4:84024721-84024740	MS.gene53839:CDS	35.0%
	ACAGCATAATCCTTATGAAC+AGG	+	chr7.4:84025075-84025094	None:intergenic	35.0%
	ATGGTTTATAGAGTCAACGT+TGG	-	chr7.4:84024655-84024674	MS.gene53839:CDS	35.0%
	CAATGATTATCCTGGTTACT+TGG	-	chr7.4:84024729-84024748	MS.gene53839:CDS	35.0%
	GATCCGCTTATCAAGAATAA+AGG	-	chr7.4:84024964-84024983	MS.gene53839:CDS	35.0%
	GCAGTAAAGTATACAACTTC+CGG	+	chr7.4:84024841-84024860	None:intergenic	35.0%
!	TATTGTTTCTCCAAGTAACC+AGG	+	chr7.4:84024742-84024761	None:intergenic	35.0%
	CATTCCAAATTACACTGCAC+CGG	-	chr7.4:84024819-84024838	MS.gene53839:CDS	40.0%
	GTTGAACCAAGTGATGATAC+AGG	-	chr7.4:84024688-84024707	MS.gene53839:CDS	40.0%
	TTATGGAATGAATGCCACAG+AGG	-	chr7.4:84024867-84024886	MS.gene53839:CDS	40.0%
	AACCAGATGCCGATGTGATA+AGG	-	chr7.4:84025028-84025047	MS.gene53839:CDS	45.0%
	ACCACTCCACCTTATCACAT+CGG	+	chr7.4:84025040-84025059	None:intergenic	45.0%
	ACTTCCGGTGCAGTGTAATT+TGG	+	chr7.4:84024826-84024845	None:intergenic	45.0%
	CGGTGCAGTGTAATTTGGAA+TGG	+	chr7.4:84024821-84024840	None:intergenic	45.0%
	CACCTTATCACATCGGCATC+TGG	+	chr7.4:84025033-84025052	None:intergenic	50.0%
	CAGATGCCGATGTGATAAGG+TGG	-	chr7.4:84025031-84025050	MS.gene53839:CDS	50.0%
	GATGTGATAAGGTGGAGTGG+TGG	-	chr7.4:84025039-84025058	MS.gene53839:CDS	50.0%
	GCCGATGTGATAAGGTGGAG+TGG	-	chr7.4:84025036-84025055	MS.gene53839:CDS	55.0%

Chromosome	Type	Strat	End	Strand	Name
chr7.4	gene	84024655	84025146	84024655	ID=MS.gene53839
chr7.4	mRNA	84024655	84025146	84024655	ID=MS.gene53839.t1;Parent=MS.gene53839
chr7.4	exon	84024655	84025146	84024655	ID=MS.gene53839.t1.exon1;Parent=MS.gene53839.t1
chr7.4	CDS	84024655	84025146	84024655	ID=cds.MS.gene53839.t1;Parent=MS.gene53839.t1

Gene Sequence

>MS.gene53839

ATGGTTTATAGAGTCAACGTTGGTGAAAATGAAGTTGAACCAAGTGATGATACAGGTATGTTTCGTAATTGGGACAATGATTATCCTGGTTACTTGGAGAAACAATATCCACTTAGTGTATCAAGTGTTTATGGAAGTGATCATCTAAACTATTTAAACAATACCATTCCAAATTACACTGCACCGGAAGTTGTATACTTTACTGCAAGAAGTTATGGAATGAATGCCACAGAGGATTATAATGTAACTTGGAATTTTGAAGTTGATTCTTCTTTTACTTATATGATAAGGTTACATTTTTGTGAGTTTGATCCGCTTATCAAGAATAAAGGTGATAGAGTTTTTCAGATATTTATCGATGATATTTTGGCTGAACCAGATGCCGATGTGATAAGGTGGAGTGGTGGTAACATGATTCCTGTTCATAAGGATTATGCTGTGAAAATGTACTCTCAAAATGGAAGCTCTGAAATTGAAAGAGTTAGTGTGTAA

Protein sequence

>MS.gene53839.t1

MVYRVNVGENEVEPSDDTGMFRNWDNDYPGYLEKQYPLSVSSVYGSDHLNYLNNTIPNYTAPEVVYFTARSYGMNATEDYNVTWNFEVDSSFTYMIRLHFCEFDPLIKNKGDRVFQIFIDDILAEPDADVIRWSGGNMIPVHKDYAVKMYSQNGSSEIERVSV