AlfalfaGEDB | MS.gene058426

M. sativa cultivar XinJiangDaYe / MS.gene058426

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058426.t1	XP_003588914.1	98.5	68	1	0	1	68	1	68	8.50E-29	136

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058426.t1	G7I8Z5	98.5	68	1	0	1	68	1	68	6.1e-29	136.0

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene050780	MS.gene058426	0.808466	3.05E-50	-1.69E-46
MS.gene050820	MS.gene058426	0.806583	7.67E-50	-1.69E-46
MS.gene051207	MS.gene058426	0.822724	2.03E-53	-1.69E-46
MS.gene051544	MS.gene058426	0.886648	2.85E-72	-1.69E-46
MS.gene052333	MS.gene058426	0.813567	2.40E-51	-1.69E-46
MS.gene052902	MS.gene058426	0.821233	4.50E-53	-1.69E-46
MS.gene053356	MS.gene058426	0.848656	5.34E-60	-1.69E-46
MS.gene053641	MS.gene058426	0.812453	4.21E-51	-1.69E-46
MS.gene053646	MS.gene058426	0.810503	1.12E-50	-1.69E-46
MS.gene053647	MS.gene058426	0.802245	6.15E-49	-1.69E-46
MS.gene054093	MS.gene058426	0.824435	8.08E-54	-1.69E-46
MS.gene05414	MS.gene058426	0.806937	6.45E-50	-1.69E-46
MS.gene056506	MS.gene058426	0.801617	8.27E-49	-1.69E-46
MS.gene058378	MS.gene058426	0.812909	3.34E-51	-1.69E-46
MS.gene058426	MS.gene058477	0.825136	5.52E-54	-1.69E-46
MS.gene058426	MS.gene059267	0.814473	1.51E-51	-1.69E-46
MS.gene058426	MS.gene060255	0.801433	9.02E-49	-1.69E-46
MS.gene058426	MS.gene063647	0.84314	1.68E-58	-1.69E-46
MS.gene058426	MS.gene064462	0.814388	1.58E-51	-1.69E-46
MS.gene058426	MS.gene065150	0.803388	3.57E-49	-1.69E-46
MS.gene058426	MS.gene065906	0.806351	8.58E-50	-1.69E-46
MS.gene058426	MS.gene067163	0.807683	4.48E-50	-1.69E-46
MS.gene058426	MS.gene071463	0.822527	2.26E-53	-1.69E-46
MS.gene058426	MS.gene071464	0.805314	1.42E-49	-1.69E-46
MS.gene058426	MS.gene071551	0.823588	1.28E-53	-1.69E-46
MS.gene058426	MS.gene071693	-0.807028	6.17E-50	-1.69E-46
MS.gene058426	MS.gene072646	0.800914	1.15E-48	-1.69E-46
MS.gene058426	MS.gene07344	0.825464	4.62E-54	-1.69E-46
MS.gene058426	MS.gene23193	0.811383	7.20E-51	-1.69E-46
MS.gene058426	MS.gene23532	0.80032	1.52E-48	-1.69E-46
MS.gene058426	MS.gene25712	0.808519	2.98E-50	-1.69E-46
MS.gene058426	MS.gene25714	0.807348	5.28E-50	-1.69E-46
MS.gene058426	MS.gene27500	0.81353	2.44E-51	-1.69E-46
MS.gene058426	MS.gene27775	0.803917	2.77E-49	-1.69E-46
MS.gene058426	MS.gene28498	0.834373	3.11E-56	-1.69E-46
MS.gene058426	MS.gene29086	0.810159	1.32E-50	-1.69E-46
MS.gene058426	MS.gene31283	0.824228	9.04E-54	-1.69E-46
MS.gene058426	MS.gene32231	0.806906	6.55E-50	-1.69E-46
MS.gene058426	MS.gene33109	0.827391	1.61E-54	-1.69E-46
MS.gene058426	MS.gene34390	0.828641	8.03E-55	-1.69E-46
MS.gene058426	MS.gene34417	0.820519	6.57E-53	-1.69E-46
MS.gene058426	MS.gene35910	0.804743	1.87E-49	-1.69E-46
MS.gene058426	MS.gene41476	0.85759	1.47E-62	-1.69E-46
MS.gene058426	MS.gene43372	0.809324	2.00E-50	-1.69E-46
MS.gene058426	MS.gene44461	0.808685	2.74E-50	-1.69E-46
MS.gene058426	MS.gene44986	0.80404	2.61E-49	-1.69E-46
MS.gene058426	MS.gene45090	0.80463	1.97E-49	-1.69E-46
MS.gene058426	MS.gene49847	0.823018	1.74E-53	-1.69E-46
MS.gene058426	MS.gene50951	0.803875	2.83E-49	-1.69E-46
MS.gene058426	MS.gene51242	0.833175	6.20E-56	-1.69E-46
MS.gene058426	MS.gene52059	0.812858	3.43E-51	-1.69E-46
MS.gene058426	MS.gene52060	0.801067	1.07E-48	-1.69E-46
MS.gene058426	MS.gene52061	0.82178	3.37E-53	-1.69E-46
MS.gene058426	MS.gene53831	0.824456	8.00E-54	-1.69E-46
MS.gene058426	MS.gene53832	0.806643	7.45E-50	-1.69E-46
MS.gene058426	MS.gene53839	0.837205	5.96E-57	-1.69E-46
MS.gene058426	MS.gene54314	0.861165	1.24E-63	-1.69E-46
MS.gene058426	MS.gene54316	0.862454	5.01E-64	-1.69E-46
MS.gene058426	MS.gene55539	0.803411	3.53E-49	-1.69E-46
MS.gene058426	MS.gene56840	0.81731	3.52E-52	-1.69E-46
MS.gene058426	MS.gene59129	0.816998	4.14E-52	-1.69E-46
MS.gene058426	MS.gene59132	0.806624	7.52E-50	-1.69E-46
MS.gene058426	MS.gene60206	0.80895	2.41E-50	-1.69E-46
MS.gene058426	MS.gene60903	0.80727	5.49E-50	-1.69E-46
MS.gene058426	MS.gene61139	0.812655	3.80E-51	-1.69E-46
MS.gene058426	MS.gene62931	0.829595	4.71E-55	-1.69E-46
MS.gene058426	MS.gene64443	0.806958	6.39E-50	-1.69E-46
MS.gene058426	MS.gene64645	0.812037	5.19E-51	-1.69E-46
MS.gene058426	MS.gene65036	0.817058	4.01E-52	-1.69E-46
MS.gene058426	MS.gene65458	0.819789	9.66E-53	-1.69E-46
MS.gene058426	MS.gene66424	0.822634	2.13E-53	-1.69E-46
MS.gene058426	MS.gene66729	0.811636	6.34E-51	-1.69E-46
MS.gene058426	MS.gene69794	0.803993	2.67E-49	-1.69E-46
MS.gene058426	MS.gene70021	0.814589	1.43E-51	-1.69E-46
MS.gene058426	MS.gene70022	0.811881	5.61E-51	-1.69E-46
MS.gene058426	MS.gene71493	0.859209	4.85E-63	-1.69E-46
MS.gene058426	MS.gene71495	0.809007	2.34E-50	-1.69E-46
MS.gene058426	MS.gene71805	0.816197	6.26E-52	-1.69E-46
MS.gene058426	MS.gene71820	0.83735	5.47E-57	-1.69E-46
MS.gene058426	MS.gene73045	0.821804	3.32E-53	-1.69E-46
MS.gene058426	MS.gene73560	0.806119	9.60E-50	-1.69E-46
MS.gene058426	MS.gene73889	0.805314	1.42E-49	-1.69E-46
MS.gene058426	MS.gene74797	0.807368	5.23E-50	-1.69E-46
MS.gene058426	MS.gene76356	0.845321	4.37E-59	-1.69E-46
MS.gene058426	MS.gene78004	0.807371	5.22E-50	-1.69E-46
MS.gene058426	MS.gene78005	0.806721	7.17E-50	-1.69E-46
MS.gene058426	MS.gene78095	0.822982	1.77E-53	-1.69E-46
MS.gene058426	MS.gene78304	0.819963	8.81E-53	-1.69E-46
MS.gene058426	MS.gene79644	0.827246	1.74E-54	-1.69E-46
MS.gene058426	MS.gene80082	0.818999	1.46E-52	-1.69E-46
MS.gene058426	MS.gene83626	0.826321	2.89E-54	-1.69E-46
MS.gene058426	MS.gene85537	0.802124	6.51E-49	-1.69E-46
MS.gene058426	MS.gene85789	0.819613	1.06E-52	-1.69E-46
MS.gene058426	MS.gene86434	0.806129	9.56E-50	-1.69E-46
MS.gene058426	MS.gene88413	0.808843	2.54E-50	-1.69E-46
MS.gene058426	MS.gene91428	0.816161	6.38E-52	-1.69E-46
MS.gene058426	MS.gene92171	0.809124	2.21E-50	-1.69E-46
MS.gene058426	MS.gene92755	0.806231	9.10E-50	-1.69E-46
MS.gene058426	MS.gene92763	0.818609	1.79E-52	-1.69E-46
MS.gene058426	MS.gene93041	0.808743	2.67E-50	-1.69E-46
MS.gene058426	MS.gene93869	0.811737	6.03E-51	-1.69E-46
MS.gene058426	MS.gene94336	0.800008	1.76E-48	-1.69E-46
MS.gene058426	MS.gene95377	0.801757	7.74E-49	-1.69E-46
MS.gene058426	MS.gene97157	0.806981	6.32E-50	-1.69E-46
MS.gene058426	MS.gene99345	0.808419	3.13E-50	-1.69E-46
MS.gene058426	MS.gene99638	0.813388	2.62E-51	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene058426.t1	MTR_1g015140	98.529	68	1	0	1	68	1	68	6.83e-44	139

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 12 sgRNAs with CRISPR-Local

Find 64 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GATGATGAATTTGCTGTTCT+TGG	0.294438	1.4:-6867082	None:intergenic
TGGTTGCTAAAATCACTTTC+AGG	0.298136	1.4:-6867106	None:intergenic
TGAGTTTCGATGACAATGAA+TGG	0.486049	1.4:+6867158	MS.gene058426:CDS
TTTCAAGCCAACGAAGTAGC+AGG	0.492097	1.4:+6867214	MS.gene058426:CDS
AAGTAGTCAGAGAGTTCAAA+TGG	0.505113	1.4:-6867136	None:intergenic
GCTGCCACCTGCTACTTCGT+TGG	0.513271	1.4:-6867221	None:intergenic
AAGGTATACAAGTATTATTA+CGG	0.515118	1.4:+6868119	MS.gene058426:intron
GCAGGTGGCAGCTACATTGA+AGG	0.517291	1.4:+6867232	MS.gene058426:CDS
AAAACTAAAGACTCAGTGGT+TGG	0.518786	1.4:-6867193	None:intergenic
AGAGTTCAAATGGCCACTGC+TGG	0.523946	1.4:-6867126	None:intergenic
CAGGTGGCAGCTACATTGAA+GGG	0.604012	1.4:+6867233	MS.gene058426:CDS
CAAGCCAACGAAGTAGCAGG+TGG	0.667226	1.4:+6867217	MS.gene058426:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	TATGTAATAAATTAATTTCA+TGG	-	chr1.4:6867588-6867607	None:intergenic	10.0%
!!!	TGTCTTTAACTTATAATTAT+TGG	+	chr1.4:6867363-6867382	MS.gene058426:intron	15.0%
!!	AAGGTATACAAGTATTATTA+CGG	+	chr1.4:6868119-6868138	MS.gene058426:intron	20.0%
!!	AATATATATACCTTGGATTA+GGG	-	chr1.4:6867559-6867578	None:intergenic	20.0%
!!	ACTAATAATATGAATATGAG+TGG	+	chr1.4:6867413-6867432	MS.gene058426:intron	20.0%
!!	ATGTACAATTTACCTTAAAA+AGG	+	chr1.4:6867890-6867909	MS.gene058426:intron	20.0%
!!	TGTACAATTTACCTTAAAAA+GGG	+	chr1.4:6867891-6867910	MS.gene058426:intron	20.0%
!!!	ATATCTTCATTCTGATTTTT+AGG	-	chr1.4:6868068-6868087	None:intergenic	20.0%
!	CAATATATATACCTTGGATT+AGG	-	chr1.4:6867560-6867579	None:intergenic	25.0%
!	CTAATCCAAGGTATATATAT+TGG	+	chr1.4:6867558-6867577	MS.gene058426:intron	25.0%
!	GAATTTGAATACTTTCATGT+AGG	-	chr1.4:6867843-6867862	None:intergenic	25.0%
!	GTATTCCAATATATATACCT+TGG	-	chr1.4:6867566-6867585	None:intergenic	25.0%
!	TAATTGGAAGAAGGAAATTA+AGG	+	chr1.4:6867442-6867461	MS.gene058426:intron	25.0%
!	TCTATATAACTGAATCTAGA+TGG	-	chr1.4:6868043-6868062	None:intergenic	25.0%
!!	AAAGTTCAAAACCCTTTTTA+AGG	-	chr1.4:6867905-6867924	None:intergenic	25.0%
!!	TAAGCCAATTTTTATCTTAC+CGG	+	chr1.4:6867273-6867292	MS.gene058426:intron	25.0%
!!	TGATTGAAATACTTGATGAT+GGG	+	chr1.4:6867492-6867511	MS.gene058426:intron	25.0%
	AAGACCGGTAAGATAAAAAT+TGG	-	chr1.4:6867280-6867299	None:intergenic	30.0%
	AGTTGAAAACTCAAACTTGT+TGG	-	chr1.4:6867866-6867885	None:intergenic	30.0%
	ATATGAGTGGAAACGATAAT+TGG	+	chr1.4:6867426-6867445	MS.gene058426:intron	30.0%
	ATATGTATATGTGTGATTGC+AGG	+	chr1.4:6867639-6867658	MS.gene058426:intron	30.0%
	CTGATTGAAATACTTGATGA+TGG	+	chr1.4:6867491-6867510	MS.gene058426:intron	30.0%
	GTGATTGCAGGAATTATTAT+AGG	+	chr1.4:6867651-6867670	MS.gene058426:intron	30.0%
	TATGTGATAACAACCTATGA+GGG	+	chr1.4:6867734-6867753	MS.gene058426:intron	30.0%
	TATTATAGGTGTTCAGTTGA+GGG	+	chr1.4:6867665-6867684	MS.gene058426:intron	30.0%
	TTATGTGATAACAACCTATG+AGG	+	chr1.4:6867733-6867752	MS.gene058426:intron	30.0%
	TTATTATAGGTGTTCAGTTG+AGG	+	chr1.4:6867664-6867683	MS.gene058426:intron	30.0%
	TTGAAAAACTAAAGACTCAG+TGG	-	chr1.4:6867200-6867219	None:intergenic	30.0%
!	GTTGAAAACTCAAACTTGTT+GGG	-	chr1.4:6867865-6867884	None:intergenic	30.0%
!	TGTTACTGTTGTCATGTTTT+AGG	+	chr1.4:6867386-6867405	MS.gene058426:intron	30.0%
!!!	CTCTCAACTCTTTTTTTTAC+AGG	-	chr1.4:6867695-6867714	None:intergenic	30.0%
	AAAACTAAAGACTCAGTGGT+TGG	-	chr1.4:6867196-6867215	None:intergenic	35.0%
	AAGTAGTCAGAGAGTTCAAA+TGG	-	chr1.4:6867139-6867158	None:intergenic	35.0%
	ATTATAGGTGTTCAGTTGAG+GGG	+	chr1.4:6867666-6867685	MS.gene058426:intron	35.0%
	TGAGTTTCGATGACAATGAA+TGG	+	chr1.4:6867158-6867177	MS.gene058426:CDS	35.0%
	TGGAAACGATAATTGGAAGA+AGG	+	chr1.4:6867433-6867452	MS.gene058426:intron	35.0%
	TGGTTGCTAAAATCACTTTC+AGG	-	chr1.4:6867109-6867128	None:intergenic	35.0%
!	GAGGAAGTATGGAAAGAAAA+TGG	+	chr1.4:6867523-6867542	MS.gene058426:intron	35.0%
!	TGTATAACCATTTGCTGACT+AGG	-	chr1.4:6867957-6867976	None:intergenic	35.0%
!!	GATGATGAATTTGCTGTTCT+TGG	-	chr1.4:6867085-6867104	None:intergenic	35.0%
	ACCTCTGCTTTCTACAAGTT+AGG	+	chr1.4:6868010-6868029	MS.gene058426:intron	40.0%
	CCTCTGCTTTCTACAAGTTA+GGG	+	chr1.4:6868011-6868030	MS.gene058426:intron	40.0%
	GTTTGAGCTAGCAAGAACTT+GGG	-	chr1.4:6867772-6867791	None:intergenic	40.0%
	TACCTCTACTTTCTGCAACA+AGG	+	chr1.4:6868100-6868119	MS.gene058426:intron	40.0%
	TACCTTGTTGCAGAAAGTAG+AGG	-	chr1.4:6868105-6868124	None:intergenic	40.0%
	TGTTTGAGCTAGCAAGAACT+TGG	-	chr1.4:6867773-6867792	None:intergenic	40.0%
	TTAAGAACAGCCCTAATCCA+AGG	+	chr1.4:6867546-6867565	MS.gene058426:intron	40.0%
!!	TACTTGATGATGGGTACAGA+TGG	+	chr1.4:6867501-6867520	MS.gene058426:intron	40.0%
	TGCAAAGCCTAGTCAGCAAA+TGG	+	chr1.4:6867947-6867966	MS.gene058426:intron	45.0%
	TGCACAAGACGAACAAAGAC+CGG	-	chr1.4:6867295-6867314	None:intergenic	45.0%
	TTTCAAGCCAACGAAGTAGC+AGG	+	chr1.4:6867214-6867233	MS.gene058426:CDS	45.0%
!	CCCTAACTTGTAGAAAGCAG+AGG	-	chr1.4:6868014-6868033	None:intergenic	45.0%
!!	GTGATTTTAGCAACCAGCAG+TGG	+	chr1.4:6867113-6867132	MS.gene058426:CDS	45.0%
!!	TTGATGATGGGTACAGATGG+AGG	+	chr1.4:6867504-6867523	MS.gene058426:intron	45.0%
!!!	ATATACATAATTTTTTTAAA+CGG	-	chr1.4:6867343-6867362	None:intergenic	5.0%
	AGAGTTCAAATGGCCACTGC+TGG	-	chr1.4:6867129-6867148	None:intergenic	50.0%
	CAGGTGGCAGCTACATTGAA+GGG	+	chr1.4:6867233-6867252	MS.gene058426:CDS	50.0%
	GAGCTAGCAAGAACTTGGGT+GGG	-	chr1.4:6867768-6867787	None:intergenic	50.0%
	GGGTACAGATGGAGGAAGTA+TGG	+	chr1.4:6867512-6867531	MS.gene058426:intron	50.0%
	TGAGCTAGCAAGAACTTGGG+TGG	-	chr1.4:6867769-6867788	None:intergenic	50.0%
	CAAGCCAACGAAGTAGCAGG+TGG	+	chr1.4:6867217-6867236	MS.gene058426:CDS	55.0%
	GCAGGTGGCAGCTACATTGA+AGG	+	chr1.4:6867232-6867251	MS.gene058426:CDS	55.0%
	GTGGGTGTGCATGCCCTCAT+AGG	-	chr1.4:6867750-6867769	None:intergenic	60.0%
!	GCTGCCACCTGCTACTTCGT+TGG	-	chr1.4:6867224-6867243	None:intergenic	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr1.4	gene	6867067	6868177	6867067	ID=MS.gene058426
chr1.4	mRNA	6867067	6868177	6867067	ID=MS.gene058426.t1;Parent=MS.gene058426
chr1.4	exon	6867067	6867271	6867067	ID=MS.gene058426.t1.exon1;Parent=MS.gene058426.t1
chr1.4	CDS	6867067	6867271	6867067	ID=cds.MS.gene058426.t1;Parent=MS.gene058426.t1
chr1.4	exon	6868122	6868177	6868122	ID=MS.gene058426.t1.exon2;Parent=MS.gene058426.t1
chr1.4	CDS	6868122	6868177	6868122	ID=cds.MS.gene058426.t1;Parent=MS.gene058426.t1

Gene Sequence

>MS.gene058426

ATGACAGAGAAAAATCCAAGAACAGCAAATTCATCATCACCTGAAAGTGATTTTAGCAACCAGCAGTGGCCATTTGAACTCTCTGACTACTTGAGTTTCGATGACAATGAATGGCTACAAAACAATCCAACCACTGAGTCTTTAGTTTTTCAAGCCAACGAAGTAGCAGGTGGCAGCTACATTGAAGGGTCTTCTAGTTCAATCAGTAAGCCAATTTTTATCTTACCGGTCTTTGTTCGTCTTGTGCAAAGACTTGATCACTAATAAATTTTGCCGTTTAAAAAAATTATGTATATTGTCTTTAACTTATAATTATTGGTGTTACTGTTGTCATGTTTTAGGAGATACTAATAATATGAATATGAGTGGAAACGATAATTGGAAGAAGGAAATTAAGGAGAGAGTTGCATTCAAAACAAAGTCACTGATTGAAATACTTGATGATGGGTACAGATGGAGGAAGTATGGAAAGAAAATGGTTAAGAACAGCCCTAATCCAAGGTATATATATTGGAATACCATGAAATTAATTTATTACATATAGTTTATATATGTATCATTATGAGTATATTATATGTATATGTGTGATTGCAGGAATTATTATAGGTGTTCAGTTGAGGGGTGTCCTGTAAAAAAAAGAGTTGAGAGAGATAATAATGATTCGAGTTATGTGATAACAACCTATGAGGGCATGCACACCCACCCAAGTTCTTGCTAGCTCAAACATTATGTGAAAAAGTTTTGCTGCATGCTCTTACAATGTTACTAGTATTCCTACATGAAAGTATTCAAATTCCCAACAAGTTTGAGTTTTCAACTGCTCATGTACAATTTACCTTAAAAAGGGTTTTGAACTTTGAAGAAGCATATATATGCAAACTGCAAAGCCTAGTCAGCAAATGGTTATACATGATTACTATTCATCTAGATTCAATTATCATTTACCTCTGCTTTCTACAAGTTAGGGTTTAGACCATCTAGATTCAGTTATATAGACACCTAAAAATCAGAATGAAGATATATCAATAACTTTTACCTCTACTTTCTGCAACAAGGTATACAAGTATTATTACGGATGCAGTATATTGAAGCATTCTACACCTTGTTTTAA

Protein sequence

>MS.gene058426.t1

MTEKNPRTANSSSPESDFSNQQWPFELSDYLSFDDNEWLQNNPTTESLVFQANEVAGGSYIEGSSSSISIQVLLRMQYIEAFYTLF