AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene53988

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53988.t1 MTR_5g071350 96.795 312 10 0 1 312 90 401 0.0 640
MS.gene53988.t1 MTR_3g464570 68.590 312 90 3 1 312 75 378 2.55e-165 465
MS.gene53988.t1 MTR_3g464570 66.667 282 86 3 31 312 1 274 2.61e-143 405
MS.gene53988.t1 MTR_5g069320 74.286 280 23 3 24 303 156 386 3.03e-142 406
MS.gene53988.t1 MTR_2g015720 51.299 308 126 4 3 310 75 358 7.06e-109 321
MS.gene53988.t1 MTR_4g088030 50.000 310 129 5 3 311 67 351 8.08e-103 305
MS.gene53988.t1 MTR_4g087730 49.191 309 133 4 3 311 79 363 1.17e-102 305
MS.gene53988.t1 MTR_4g079700 47.896 309 136 5 2 310 80 363 1.30e-100 300
MS.gene53988.t1 MTR_3g049330 47.742 310 140 5 2 311 64 351 1.82e-100 299
MS.gene53988.t1 MTR_2g091175 46.278 309 141 4 3 311 84 367 7.81e-100 298
MS.gene53988.t1 MTR_3g035830 45.130 308 145 4 3 310 65 348 5.59e-97 290
MS.gene53988.t1 MTR_2g073510 47.712 306 138 6 2 307 67 350 1.04e-96 290
MS.gene53988.t1 MTR_3g021060 45.455 308 144 4 3 310 80 363 4.89e-96 288
MS.gene53988.t1 MTR_3g021090 46.053 304 140 4 3 306 65 344 6.14e-96 290
MS.gene53988.t1 MTR_5g034810 46.278 309 144 7 3 311 67 353 1.36e-93 281
MS.gene53988.t1 MTR_2g073500 43.910 312 140 6 2 311 68 346 5.68e-87 265
MS.gene53988.t1 MTR_1g112210 40.260 308 161 4 2 306 91 378 1.65e-86 265
MS.gene53988.t1 MTR_1g050448 38.387 310 171 3 2 310 85 375 1.05e-83 257
MS.gene53988.t1 MTR_5g033800 37.461 323 175 5 1 311 177 484 5.37e-81 254
MS.gene53988.t1 MTR_1g112210 39.423 312 153 5 2 310 91 369 6.94e-81 249
MS.gene53988.t1 MTR_7g093450 38.957 326 165 7 2 311 281 588 1.67e-79 253
MS.gene53988.t1 MTR_4g121560 39.938 323 165 6 2 311 148 454 3.71e-79 248
MS.gene53988.t1 MTR_5g059400 38.154 325 174 6 1 312 207 517 1.25e-78 249
MS.gene53988.t1 MTR_1g116130 38.154 325 174 6 1 312 207 517 1.25e-78 249
MS.gene53988.t1 MTR_0136s0010 37.423 326 170 7 2 311 203 510 1.53e-77 246
MS.gene53988.t1 MTR_1g111010 41.346 312 145 8 2 311 67 342 6.35e-75 234
MS.gene53988.t1 MTR_8g069790 36.196 326 175 6 1 311 210 517 2.06e-74 238
MS.gene53988.t1 MTR_4g016940 37.618 319 178 7 2 310 120 427 3.94e-74 234
MS.gene53988.t1 MTR_4g126950 37.267 322 174 6 1 310 141 446 8.88e-74 234
MS.gene53988.t1 MTR_3g070900 38.629 321 174 6 2 310 140 449 1.63e-72 231
MS.gene53988.t1 MTR_7g109550 36.562 320 170 8 2 310 170 467 2.22e-71 228
MS.gene53988.t1 MTR_5g082880 36.615 325 180 7 2 311 199 512 4.28e-69 223
MS.gene53988.t1 MTR_8g463790 35.202 321 182 7 2 311 105 410 1.29e-68 219
MS.gene53988.t1 MTR_4g014440 35.185 324 185 7 3 312 97 409 5.32e-68 218
MS.gene53988.t1 MTR_7g094750 35.312 337 164 9 2 311 124 433 1.48e-67 218
MS.gene53988.t1 MTR_3g071650 36.810 326 175 8 2 312 123 432 2.36e-66 214
MS.gene53988.t1 MTR_7g068920 37.812 320 176 8 2 310 114 421 2.68e-66 214
MS.gene53988.t1 MTR_5g083260 36.503 326 176 7 2 312 117 426 2.18e-64 209
MS.gene53988.t1 MTR_1g058380 34.862 327 175 8 2 311 90 395 5.69e-64 207
MS.gene53988.t1 MTR_7g070060 34.365 323 187 6 3 311 99 410 8.37e-64 207
MS.gene53988.t1 MTR_2g022560 35.347 331 170 10 2 311 130 437 1.93e-62 204
MS.gene53988.t1 MTR_4g014430 35.238 315 182 5 2 307 142 443 2.21e-60 199
MS.gene53988.t1 MTR_7g070060 32.390 318 189 7 9 311 2 308 3.96e-56 184
MS.gene53988.t1 MTR_3g090120 32.716 324 175 8 2 311 166 460 7.94e-56 187
MS.gene53988.t1 MTR_5g033800 38.424 203 114 2 1 192 177 379 4.47e-51 173
MS.gene53988.t1 MTR_8g093530 31.595 326 176 6 2 311 123 417 4.93e-50 172
MS.gene53988.t1 MTR_7g094750 36.058 208 112 4 2 192 124 327 3.28e-44 154
MS.gene53988.t1 MTR_1g076690 31.288 326 188 11 2 306 256 566 3.32e-39 145
MS.gene53988.t1 MTR_7g019120 31.366 322 185 12 3 306 113 416 3.27e-37 137
MS.gene53988.t1 MTR_5g035150 30.841 321 195 10 2 306 118 427 1.81e-36 135
MS.gene53988.t1 MTR_5g035090 29.907 321 196 9 2 306 116 423 2.11e-36 135
MS.gene53988.t1 MTR_5g035080 30.094 319 199 9 2 306 108 416 2.08e-35 133
MS.gene53988.t1 MTR_6g016880 29.321 324 197 10 3 310 112 419 3.35e-35 132
MS.gene53988.t1 MTR_7g070060 32.487 197 125 2 3 191 99 295 3.70e-35 129
MS.gene53988.t1 MTR_8g093870 28.863 343 175 12 1 310 166 472 1.17e-34 131
MS.gene53988.t1 MTR_8g104970 29.063 320 203 11 1 306 152 461 2.67e-33 127
MS.gene53988.t1 MTR_8g027900 30.745 322 200 11 2 310 244 555 6.86e-33 127
MS.gene53988.t1 MTR_4g125060 29.940 334 177 16 4 310 168 471 8.63e-33 126
MS.gene53988.t1 MTR_4g125060 29.940 334 177 16 4 310 207 510 8.69e-33 127
MS.gene53988.t1 MTR_4g125060 29.940 334 177 16 4 310 236 539 1.15e-32 127
MS.gene53988.t1 MTR_6g061710 27.523 327 201 10 2 307 200 511 2.61e-32 125
MS.gene53988.t1 MTR_2g016060 30.678 339 183 16 1 310 86 401 5.40e-31 120
MS.gene53988.t1 MTR_3g109840 27.273 330 194 10 1 306 104 411 1.16e-30 120
MS.gene53988.t1 MTR_7g033230 26.791 321 209 9 1 306 296 605 1.81e-30 121
MS.gene53988.t1 MTR_7g033230 26.586 331 197 10 1 306 366 675 5.28e-30 120
MS.gene53988.t1 MTR_5g035070 29.283 321 200 10 2 306 120 429 6.47e-30 118
MS.gene53988.t1 MTR_6g016875 27.405 343 182 12 3 310 98 408 9.35e-30 117
MS.gene53988.t1 MTR_8g099300 27.300 337 185 11 2 306 107 415 2.06e-29 116
MS.gene53988.t1 MTR_4g092830 26.752 314 194 10 12 306 92 388 2.07e-27 111
MS.gene53988.t1 MTR_6g007777 27.964 329 196 12 2 310 150 457 3.11e-27 111
MS.gene53988.t1 MTR_3g021150 43.333 120 55 2 154 273 1 107 5.17e-27 102
MS.gene53988.t1 MTR_6g016240 28.107 338 187 16 1 310 107 416 5.58e-27 109
MS.gene53988.t1 MTR_2g022580 38.400 125 64 3 29 140 2 126 1.19e-23 94.4
MS.gene53988.t1 MTR_7g019140 64.286 56 20 0 3 58 113 168 1.60e-19 84.3
MS.gene53988.t1 MTR_5g035120 52.542 59 28 0 1 59 107 165 6.28e-15 72.0
MS.gene53988.t1 MTR_5g035140 22.426 272 182 10 51 306 3 261 2.42e-11 63.5
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene53988.t1 AT5G58600 60.577 312 115 2 1 312 99 402 1.95e-149 426
MS.gene53988.t1 AT2G30010 58.199 311 125 3 3 312 92 398 1.61e-137 395
MS.gene53988.t1 AT2G30010 58.199 311 125 3 3 312 135 441 2.29e-137 396
MS.gene53988.t1 AT1G29050 49.518 311 127 4 3 310 95 378 3.50e-107 317
MS.gene53988.t1 AT2G34070 48.867 309 133 5 3 310 99 383 4.84e-103 307
MS.gene53988.t1 AT2G42570 47.249 309 139 5 3 311 81 365 2.14e-101 302
MS.gene53988.t1 AT2G31110 47.078 308 139 4 3 310 79 362 2.21e-99 297
MS.gene53988.t1 AT5G58600 63.874 191 69 0 1 191 99 289 7.04e-98 290
MS.gene53988.t1 AT2G30900 48.243 313 139 8 2 312 76 367 8.68e-97 290
MS.gene53988.t1 AT1G78710 44.373 311 147 6 3 311 71 357 1.86e-91 276
MS.gene53988.t1 AT3G14850 44.118 306 150 5 2 307 67 351 1.20e-90 274
MS.gene53988.t1 AT2G34070 47.985 273 117 5 39 310 1 249 2.90e-87 262
MS.gene53988.t1 AT3G54260 41.234 308 158 4 2 306 83 370 2.74e-86 264
MS.gene53988.t1 AT1G60790 38.889 324 169 6 2 311 225 533 7.49e-80 252
MS.gene53988.t1 AT5G06700 38.580 324 169 7 2 311 288 595 6.23e-79 251
MS.gene53988.t1 AT5G20590 39.319 323 166 8 2 311 175 480 1.09e-77 245
MS.gene53988.t1 AT5G49340 40.120 334 152 11 2 311 133 442 1.06e-76 242
MS.gene53988.t1 AT3G12060 37.615 327 168 8 2 311 229 536 1.59e-76 244
MS.gene53988.t1 AT5G01360 37.500 320 178 7 2 310 70 378 1.24e-72 229
MS.gene53988.t1 AT3G62390 35.093 322 179 7 2 312 171 473 4.01e-72 230
MS.gene53988.t1 AT5G01360 37.500 320 178 7 2 310 126 434 6.67e-72 229
MS.gene53988.t1 AT3G14850 41.636 269 136 5 39 307 1 248 1.47e-71 222
MS.gene53988.t1 AT3G55990 38.037 326 171 6 2 311 174 484 2.54e-71 229
MS.gene53988.t1 AT3G11570 37.615 327 166 7 2 311 115 420 1.19e-69 223
MS.gene53988.t1 AT2G40160 35.276 326 185 8 2 312 111 425 7.45e-68 218
MS.gene53988.t1 AT2G40160 35.276 326 185 8 2 312 111 425 1.94e-67 217
MS.gene53988.t1 AT2G40150 36.170 329 177 7 2 310 105 420 1.70e-66 214
MS.gene53988.t1 AT1G73140 34.985 323 180 8 2 311 101 406 2.71e-66 213
MS.gene53988.t1 AT2G31110 46.957 230 98 4 81 310 9 214 2.90e-66 207
MS.gene53988.t1 AT5G06230 37.423 326 168 8 2 311 60 365 1.48e-65 210
MS.gene53988.t1 AT5G06230 37.423 326 168 8 2 311 101 406 4.39e-65 210
MS.gene53988.t1 AT3G11030 36.086 327 177 7 2 312 140 450 7.29e-65 211
MS.gene53988.t1 AT5G01620 34.906 318 185 5 2 310 145 449 2.53e-64 209
MS.gene53988.t1 AT5G01620 34.906 318 185 5 2 310 145 449 2.53e-64 209
MS.gene53988.t1 AT5G01620 34.906 318 185 5 2 310 153 457 3.71e-64 209
MS.gene53988.t1 AT2G31110 49.457 184 92 1 3 186 79 261 5.49e-64 203
MS.gene53988.t1 AT2G40320 35.890 326 178 7 2 312 115 424 1.84e-63 206
MS.gene53988.t1 AT2G38320 33.437 323 190 7 3 311 11 322 5.16e-63 202
MS.gene53988.t1 AT2G38320 33.437 323 190 7 3 311 94 405 4.22e-62 202
MS.gene53988.t1 AT5G19160 35.241 332 172 10 3 311 133 444 6.38e-62 204
MS.gene53988.t1 AT2G30900 52.406 187 86 3 2 186 76 261 1.71e-61 197
MS.gene53988.t1 AT3G06080 35.061 328 175 10 3 311 137 445 4.59e-61 201
MS.gene53988.t1 AT1G48880 32.198 323 176 8 2 311 148 440 4.59e-52 177
MS.gene53988.t1 AT5G20590 39.409 203 111 3 2 192 175 377 4.53e-48 165
MS.gene53988.t1 AT3G62390 36.634 202 117 4 2 192 171 372 3.23e-46 161
MS.gene53988.t1 AT1G78710 33.439 314 124 11 3 311 64 297 2.85e-44 153
MS.gene53988.t1 AT5G06230 37.745 204 114 3 2 192 101 304 1.43e-43 152
MS.gene53988.t1 AT5G19160 36.453 203 116 3 3 192 133 335 2.32e-43 152
MS.gene53988.t1 AT2G40160 35.500 200 119 3 2 191 111 310 1.06e-41 147
MS.gene53988.t1 AT3G06080 34.314 204 119 4 3 192 137 339 2.17e-37 136
MS.gene53988.t1 AT4G25360 31.402 328 187 12 2 306 208 520 3.20e-37 139
MS.gene53988.t1 AT4G25360 31.402 328 187 12 2 306 208 520 3.20e-37 139
MS.gene53988.t1 AT5G64020 30.725 345 174 14 3 310 92 408 2.38e-36 135
MS.gene53988.t1 AT5G51640 30.395 329 190 11 2 306 171 484 1.08e-35 135
MS.gene53988.t1 AT2G14530 29.141 326 178 11 12 310 107 406 1.82e-35 132
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 154 457 5.26e-35 132
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 230 533 8.71e-35 132
MS.gene53988.t1 AT1G01430 28.571 329 204 9 2 311 132 448 1.08e-34 131
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 248 551 1.09e-34 132
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 248 551 1.09e-34 132
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 248 551 1.09e-34 132
MS.gene53988.t1 AT5G20680 29.217 332 182 12 4 310 248 551 1.09e-34 132
MS.gene53988.t1 AT1G70230 27.778 324 195 10 2 310 112 411 2.79e-33 127
MS.gene53988.t1 AT2G37720 28.986 345 168 12 3 310 178 482 4.79e-32 124
MS.gene53988.t1 AT5G15900 29.305 331 189 11 3 310 101 409 2.14e-31 122
MS.gene53988.t1 AT5G15900 29.305 331 189 11 3 310 120 428 3.23e-31 122
MS.gene53988.t1 AT5G64470 29.268 328 189 14 2 306 89 396 4.12e-30 118
MS.gene53988.t1 AT4G01080 27.879 330 188 10 1 306 123 426 1.29e-29 117
MS.gene53988.t1 AT5G64470 28.834 326 187 13 2 306 89 390 3.71e-29 115
MS.gene53988.t1 AT5G15890 27.812 320 193 9 3 306 221 518 1.74e-28 115
MS.gene53988.t1 AT3G28150 27.744 328 196 11 3 310 104 410 3.55e-28 113
MS.gene53988.t1 AT4G11090 26.791 321 207 9 2 306 113 421 5.76e-28 112
MS.gene53988.t1 AT3G02440 29.305 331 180 15 3 306 161 464 1.40e-27 112
MS.gene53988.t1 AT3G02440 29.305 331 180 15 3 306 169 472 1.44e-27 112
MS.gene53988.t1 AT4G23790 28.267 329 194 11 2 306 113 423 4.90e-27 110
MS.gene53988.t1 AT5G64470 27.778 216 132 8 2 198 89 299 7.52e-15 74.3
MS.gene53988.t1 AT5G64470 27.778 216 132 8 2 198 89 299 9.12e-15 73.9

Find 79 sgRNAs with CRISPR-Local

Find 206 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACTAAATTCACCACTGTAAA+TGG 0.183953 5.2:-60318901 None:intergenic
TCTCTCCACTTTCACATTCT+TGG 0.215880 5.2:+60315974 MS.gene53988:CDS
TAGAACCCATGTGTTATTTC+AGG 0.260292 5.2:+60317185 MS.gene53988:CDS
GTATGAAAACATGGGCTAAT+TGG 0.283621 5.2:+60317139 MS.gene53988:CDS
GGTTCTACTTCTGTCAATAT+TGG 0.289055 5.2:-60317169 None:intergenic
GCTACCAAACGATCCATATC+AGG 0.293082 5.2:-60317105 None:intergenic
TGTAGCCATTGGTGTCTTCC+TGG 0.300852 5.2:+60318960 MS.gene53988:CDS
TTCCTGGTTTGCCTGATACT+TGG 0.320303 5.2:+60318976 MS.gene53988:CDS
ACAAATGAGAGTAGTTAATA+TGG 0.332485 5.2:+60318800 MS.gene53988:CDS
AGGTGGGATTATGTAGAATT+AGG 0.337120 5.2:+60317069 MS.gene53988:intron
ACATCAACATCTACTAAATA+AGG 0.339654 5.2:-60316798 None:intergenic
GTGGTTTCCAACGGTATTTA+AGG 0.345458 5.2:-60315531 None:intergenic
TATGGATCGTTTGGTAGCTT+TGG 0.346986 5.2:+60317110 MS.gene53988:CDS
AGCAGTTGATTCCAAGTATC+AGG 0.353139 5.2:-60318987 None:intergenic
ATGTAGAGACACAATTGGTT+AGG 0.385952 5.2:+60315943 MS.gene53988:CDS
CTACAATCAGCTGAGGGATC+AGG 0.397366 5.2:-60318942 None:intergenic
TATGAAAACATGGGCTAATT+GGG 0.412563 5.2:+60317140 MS.gene53988:CDS
ACCAATTGTGTCTCTACATT+AGG 0.416285 5.2:-60315939 None:intergenic
TTACCCTGATATGGATCGTT+TGG 0.418196 5.2:+60317101 MS.gene53988:CDS
GATCAGGGTCAGCTTTCTGC+TGG 0.433002 5.2:-60318926 None:intergenic
GAATTTAATTGTCAAATGAA+TGG 0.456754 5.2:+60315490 None:intergenic
TATTTAAGGTAATCAGAATC+AGG 0.460533 5.2:-60315517 None:intergenic
TTGTGTAATGGTAGGTTCAA+TGG 0.461038 5.2:+60315810 MS.gene53988:intron
CGTTTGGTAGCTTTGGAAAG+AGG 0.463824 5.2:+60317117 MS.gene53988:CDS
TGTAGAGACACAATTGGTTA+GGG 0.465159 5.2:+60315944 MS.gene53988:CDS
TCTGTTGTTGTGCCACTGAT+TGG 0.469702 5.2:-60318747 None:intergenic
CTGATTACCTTAAATACCGT+TGG 0.477522 5.2:+60315524 MS.gene53988:CDS
ATATGGTATATGCAGGACTA+TGG 0.498461 5.2:+60316750 MS.gene53988:intron
CACTCAATTGTGAGCTCCCA+AGG 0.498988 5.2:+60315551 MS.gene53988:CDS
AGTGGCACAACAACAGACTT+TGG 0.502553 5.2:+60318753 MS.gene53988:CDS
TTATCAGAAATGAGGAAAGA+TGG 0.503627 5.2:+60318870 MS.gene53988:CDS
ATGTTTGTTGGTGATTCGTT+AGG 0.505244 5.2:+60315870 MS.gene53988:CDS
TGGGATTATGTAGAATTAGG+AGG 0.519702 5.2:+60317072 MS.gene53988:CDS
TACAATCAGCTGAGGGATCA+GGG 0.525364 5.2:-60318941 None:intergenic
CCTCAGCTGATTGTAGCCAT+TGG 0.526005 5.2:+60318949 MS.gene53988:CDS
ACCTAATGTAGAGACACAAT+TGG 0.528684 5.2:+60315938 MS.gene53988:CDS
GTAGATGTTGATGTGGTCCA+AGG 0.533368 5.2:+60316807 MS.gene53988:CDS
TGGAAAGAGGTATGAAAACA+TGG 0.533428 5.2:+60317130 MS.gene53988:CDS
ACTCACCAAGAATGTGAAAG+TGG 0.541721 5.2:-60315979 None:intergenic
GGGATTATGTAGAATTAGGA+GGG 0.546280 5.2:+60317073 MS.gene53988:CDS
AATTCACCACTGTAAATGGA+AGG 0.547293 5.2:-60318897 None:intergenic
GTGATGTCAAGAAGATATGC+AGG 0.548563 5.2:-60318843 None:intergenic
TTATTTAGTAGATGTTGATG+TGG 0.549664 5.2:+60316800 MS.gene53988:CDS
TTCCAAGTATCAGGCAAACC+AGG 0.553910 5.2:-60318978 None:intergenic
CTTGATGAGGCAGATAAGAA+TGG 0.554006 5.2:+60316846 MS.gene53988:CDS
TTGTTGGTGATTCGTTAGGA+AGG 0.556705 5.2:+60315874 MS.gene53988:CDS
GGACATTGGTGGAGTCATCA+AGG 0.558127 5.2:+60316909 MS.gene53988:CDS
TCACAATGTTATCAGAAATG+AGG 0.559566 5.2:+60318862 MS.gene53988:CDS
ATTCGTTAGGAAGGAATCAA+TGG 0.559645 5.2:+60315883 MS.gene53988:CDS
ATCAGGGTCAGCTTTCTGCT+GGG 0.559672 5.2:-60318925 None:intergenic
AGTCATCAAGGATCACTTCA+AGG 0.572350 5.2:+60316921 MS.gene53988:CDS
TGTTCAACACAGGACATTGG+TGG 0.574863 5.2:+60316898 MS.gene53988:CDS
CACAATTGAGTGGTTTCCAA+CGG 0.579725 5.2:-60315540 None:intergenic
AATGAATGGAATTCAGCTGT+TGG 0.581780 5.2:+60318675 MS.gene53988:CDS
GAGTCCTTACTCATAATGTG+TGG 0.582644 5.2:-60317217 None:intergenic
CAGATAAGAATGGTGACACA+TGG 0.592161 5.2:+60316856 MS.gene53988:CDS
ACGACGACAAAGAATTGCTA+TGG 0.598313 5.2:+60318714 MS.gene53988:CDS
GGCACAACAACAGACTTTGG+AGG 0.599898 5.2:+60318756 MS.gene53988:CDS
CTACCAAACGATCCATATCA+GGG 0.604629 5.2:-60317104 None:intergenic
ATGGAAGAATGTTGATGTGT+TGG 0.608768 5.2:+60316875 MS.gene53988:CDS
CTTGGGAGCTCACAATTGAG+TGG 0.611295 5.2:-60315550 None:intergenic
GAAAACATGGGCTAATTGGG+TGG 0.618678 5.2:+60317143 MS.gene53988:CDS
TAGATGTTGATGTGGTCCAA+GGG 0.619218 5.2:+60316808 MS.gene53988:CDS
ATTCACCACTGTAAATGGAA+GGG 0.620444 5.2:-60318896 None:intergenic
ACAACAACAGACTTTGGAGG+AGG 0.621376 5.2:+60318759 MS.gene53988:CDS
AGTCCTTACTCATAATGTGT+GGG 0.625224 5.2:-60317216 None:intergenic
AAATTGCCTGAAATAACACA+TGG 0.627656 5.2:-60317191 None:intergenic
GTCATCAAGGATCACTTCAA+GGG 0.627727 5.2:+60316922 MS.gene53988:CDS
GATGTGTTGGTGTTCAACAC+AGG 0.628377 5.2:+60316888 MS.gene53988:CDS
GGAAAGAGGTATGAAAACAT+GGG 0.632297 5.2:+60317131 MS.gene53988:CDS
AAGAATGTGAAAGTGGAGAG+AGG 0.650847 5.2:-60315972 None:intergenic
CCAATGGCTACAATCAGCTG+AGG 0.653939 5.2:-60318949 None:intergenic
TGGTGTTCAACACAGGACAT+TGG 0.669188 5.2:+60316895 MS.gene53988:CDS
GGTGAAACAGCACCAATCAG+TGG 0.670747 5.2:+60318735 MS.gene53988:CDS
GCAAACCAGGAAGACACCAA+TGG 0.684241 5.2:-60318965 None:intergenic
GGACACCCTTCCATTTACAG+TGG 0.697469 5.2:+60318891 MS.gene53988:CDS
GTAGAGACACAATTGGTTAG+GGG 0.699684 5.2:+60315945 MS.gene53988:CDS
CAATGGCTACAATCAGCTGA+GGG 0.700420 5.2:-60318948 None:intergenic
AATTGCCTGAAATAACACAT+GGG 0.716075 5.2:-60317190 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! AACAAATATAGTAAATAAAT+TGG + chr5.2:60317963-60317982 MS.gene53988:intron 10.0%
!! TTAATTTAGTGATTTAAAAA+TGG + chr5.2:60317337-60317356 MS.gene53988:intron 10.0%
!!! AAAATTCTTGAAAATATTTT+GGG - chr5.2:60316628-60316647 None:intergenic 10.0%
!!! ATGTATAAATTATTTCTATT+GGG - chr5.2:60318431-60318450 None:intergenic 10.0%
!!! TATGTATAAATTATTTCTAT+TGG - chr5.2:60318432-60318451 None:intergenic 10.0%
!! AAGAATCAATTATTCATAAT+TGG - chr5.2:60317265-60317284 None:intergenic 15.0%
!! AATTAAATAATATGCATATG+AGG + chr5.2:60316599-60316618 MS.gene53988:intron 15.0%
!! ATTAAATAATATGCATATGA+GGG + chr5.2:60316600-60316619 MS.gene53988:intron 15.0%
!! ATTAAATTGTTTATTCAAAC+AGG + chr5.2:60318490-60318509 MS.gene53988:intron 15.0%
!! ATTATTTGTAACTTGAAAAT+AGG + chr5.2:60317304-60317323 MS.gene53988:intron 15.0%
!! TCATATGCATATTATTTAAT+TGG - chr5.2:60316600-60316619 None:intergenic 15.0%
!! TTAAATTGTTTATTCAAACA+GGG + chr5.2:60318491-60318510 MS.gene53988:intron 15.0%
!! TTATTTGTAACTTGAAAATA+GGG + chr5.2:60317305-60317324 MS.gene53988:intron 15.0%
!! TTTGAATTTGTACTTAAAAA+AGG + chr5.2:60317009-60317028 MS.gene53988:intron 15.0%
!!! AAATTCTTGAAAATATTTTG+GGG - chr5.2:60316627-60316646 None:intergenic 15.0%
!!! ATATTTTCCTCAATATAAAA+CGG - chr5.2:60317921-60317940 None:intergenic 15.0%
!!! CAAAATTCTTGAAAATATTT+TGG - chr5.2:60316629-60316648 None:intergenic 15.0%
!!! TAACAAAAAGTTTTAAATTG+TGG + chr5.2:60316035-60316054 MS.gene53988:intron 15.0%
!!! TAATTCCTGTTTTAATTATT+AGG - chr5.2:60318517-60318536 None:intergenic 15.0%
!!! TATTTTAGTGATGTTAAAAT+TGG + chr5.2:60316722-60316741 MS.gene53988:intron 15.0%
!!! TCATTTTTTCAAATTATTAC+CGG + chr5.2:60316182-60316201 MS.gene53988:intron 15.0%
!!! TTAAAAAAGGTTGATTTTTT+GGG + chr5.2:60317022-60317041 MS.gene53988:intron 15.0%
!! AAAGAATTAGGAAGAAAAAA+TGG + chr5.2:60317697-60317716 MS.gene53988:intron 20.0%
!! AATAAGCTAAAAACAACTTA+TGG - chr5.2:60318334-60318353 None:intergenic 20.0%
!! AATCAAATTACAACAAGATA+TGG - chr5.2:60317534-60317553 None:intergenic 20.0%
!! AATTTGATTATTCTATTCTG+AGG + chr5.2:60317545-60317564 MS.gene53988:intron 20.0%
!! ATAAGCTAAAAACAACTTAT+GGG - chr5.2:60318333-60318352 None:intergenic 20.0%
!! ATCAAATTACAACAAGATAT+GGG - chr5.2:60317533-60317552 None:intergenic 20.0%
!! CATAAGAAAGATAAAGAATT+AGG + chr5.2:60317685-60317704 MS.gene53988:intron 20.0%
!! GAATCATTAGATAGATATAA+GGG + chr5.2:60316573-60316592 MS.gene53988:intron 20.0%
!! TGAATCATTAGATAGATATA+AGG + chr5.2:60316572-60316591 MS.gene53988:intron 20.0%
!! TGTTAAAATTGGATATGATA+TGG + chr5.2:60316733-60316752 MS.gene53988:intron 20.0%
!! TTAAATAATATGCATATGAG+GGG + chr5.2:60316601-60316620 MS.gene53988:intron 20.0%
!! TTATATGTAGCAAAAATGAA+TGG + chr5.2:60318661-60318680 MS.gene53988:intron 20.0%
!! TTTGGATTCATTTATTTACT+TGG + chr5.2:60318258-60318277 MS.gene53988:intron 20.0%
!!! AGCAAAATATTGTGTTTTTA+AGG + chr5.2:60315744-60315763 MS.gene53988:intron 20.0%
!!! CTTAAAAAAGGTTGATTTTT+TGG + chr5.2:60317021-60317040 MS.gene53988:intron 20.0%
!!! GGCTTTATTACATTAATATT+TGG + chr5.2:60318571-60318590 MS.gene53988:intron 20.0%
!!! GTAAATTTAGTTTGACTTTA+GGG - chr5.2:60317426-60317445 None:intergenic 20.0%
!!! TATTTTCCTCAATATAAAAC+GGG - chr5.2:60317920-60317939 None:intergenic 20.0%
!!! TGTAAATTTAGTTTGACTTT+AGG - chr5.2:60317427-60317446 None:intergenic 20.0%
!!! TTTTAAACAAAAGTTATGCT+AGG + chr5.2:60318613-60318632 MS.gene53988:intron 20.0%
!!! TTTTCAAGAATTTTGATACA+TGG + chr5.2:60316634-60316653 MS.gene53988:intron 20.0%
!!! TTTTTTCATTATACTCGATT+GGG - chr5.2:60318185-60318204 None:intergenic 20.0%
!!! TTTTTTTCATTATACTCGAT+TGG - chr5.2:60318186-60318205 None:intergenic 20.0%
! AATCTTACCAACAAAAAACT+TGG - chr5.2:60316370-60316389 None:intergenic 25.0%
! ACAAATGAGAGTAGTTAATA+TGG + chr5.2:60318800-60318819 MS.gene53988:CDS 25.0%
! ACATCAACATCTACTAAATA+AGG - chr5.2:60316801-60316820 None:intergenic 25.0%
! AGTATGCTGAATAAATTTCT+AGG + chr5.2:60317997-60318016 MS.gene53988:intron 25.0%
! ATCTTACCAACAAAAAACTT+GGG - chr5.2:60316369-60316388 None:intergenic 25.0%
! GTTGTGATCATTGATATATT+CGG + chr5.2:60316057-60316076 MS.gene53988:intron 25.0%
! TTATTTAGTAGATGTTGATG+TGG + chr5.2:60316800-60316819 MS.gene53988:CDS 25.0%
! TTGACAGTAAAAATGTAACA+TGG + chr5.2:60318550-60318569 MS.gene53988:intron 25.0%
!! ATGAATGAAACTTTAGAAAG+AGG - chr5.2:60316469-60316488 None:intergenic 25.0%
!! ATTGAACATAATCAAGTTGT+AGG - chr5.2:60315665-60315684 None:intergenic 25.0%
!! CTTTAGGGAAAAAGTTAATT+TGG - chr5.2:60317411-60317430 None:intergenic 25.0%
!! TATTTAAGGTAATCAGAATC+AGG - chr5.2:60315520-60315539 None:intergenic 25.0%
!! TCTTTCTTATGTGTTTTCAT+TGG - chr5.2:60317676-60317695 None:intergenic 25.0%
!! TGAATGAAACTTTAGAAAGA+GGG - chr5.2:60316468-60316487 None:intergenic 25.0%
!!! ACAAGCATTGATATGTAATA+AGG + chr5.2:60318232-60318251 MS.gene53988:intron 25.0%
!!! CAAGCATTGATATGTAATAA+GGG + chr5.2:60318233-60318252 MS.gene53988:intron 25.0%
!!! CAATATTTTGCTTTAAAGCA+CGG - chr5.2:60315736-60315755 None:intergenic 25.0%
!!! GATTTAGATTTTGGTTCAAA+AGG + chr5.2:60316386-60316405 MS.gene53988:intron 25.0%
!!! TGTTGGTAAGATTTAGATTT+TGG + chr5.2:60316377-60316396 MS.gene53988:intron 25.0%
AAATTGCCTGAAATAACACA+TGG - chr5.2:60317194-60317213 None:intergenic 30.0%
AATTGCCTGAAATAACACAT+GGG - chr5.2:60317193-60317212 None:intergenic 30.0%
ACAAAAGTTATGCTAGGATT+TGG + chr5.2:60318619-60318638 MS.gene53988:intron 30.0%
ACTAAATTCACCACTGTAAA+TGG - chr5.2:60318904-60318923 None:intergenic 30.0%
ATAGTGTGAAATGCAAAATG+AGG - chr5.2:60316309-60316328 None:intergenic 30.0%
ATGGTAAAACTCTTTCTAAG+AGG - chr5.2:60317840-60317859 None:intergenic 30.0%
GCTTAGCATAATTTGAATGA+TGG + chr5.2:60315784-60315803 MS.gene53988:intron 30.0%
GTCAAGAATAGTCAAAAGAA+AGG - chr5.2:60317503-60317522 None:intergenic 30.0%
TCACAATGTTATCAGAAATG+AGG + chr5.2:60318862-60318881 MS.gene53988:CDS 30.0%
TCATTCATGATATCACAAAG+AGG + chr5.2:60316481-60316500 MS.gene53988:intron 30.0%
TGAAATGCAAAATGAGGTAT+TGG - chr5.2:60316303-60316322 None:intergenic 30.0%
TGATATATTCGGTAGAATTG+CGG + chr5.2:60316068-60316087 MS.gene53988:intron 30.0%
TTAAATCATGCAACAACACA+TGG + chr5.2:60317736-60317755 MS.gene53988:intron 30.0%
TTAGTTGAGTATGACCAAAT+TGG - chr5.2:60317863-60317882 None:intergenic 30.0%
TTATCAGAAATGAGGAAAGA+TGG + chr5.2:60318870-60318889 MS.gene53988:CDS 30.0%
TTCATAATTCTGTGACAAGA+TGG - chr5.2:60317599-60317618 None:intergenic 30.0%
TTGCATAGTCAATATTCTAC+AGG - chr5.2:60316693-60316712 None:intergenic 30.0%
! AAGAGTTTTACCATCCAATT+TGG + chr5.2:60317846-60317865 MS.gene53988:intron 30.0%
! ATGATACCCGTTTTATATTG+AGG + chr5.2:60317911-60317930 MS.gene53988:intron 30.0%
! GAGTTTTTGTTGAAGATGAA+AGG + chr5.2:60315837-60315856 MS.gene53988:CDS 30.0%
! GGAAACAACTATTTTATTGC+TGG - chr5.2:60316447-60316466 None:intergenic 30.0%
! TATGAAAACATGGGCTAATT+GGG + chr5.2:60317140-60317159 MS.gene53988:CDS 30.0%
! TGTGTTGTTGCATGATTTAA+AGG - chr5.2:60317736-60317755 None:intergenic 30.0%
!!! AAATTTTTTGGCTTGCTTGT+TGG + chr5.2:60317571-60317590 MS.gene53988:intron 30.0%
!!! AAGAATTTTGAGACTTGATG+AGG + chr5.2:60316833-60316852 MS.gene53988:CDS 30.0%
!!! AGGTAGGGTCATATTTTTTT+TGG + chr5.2:60316501-60316520 MS.gene53988:intron 30.0%
!!! ATTCTGAGGCTGAAATTTTT+TGG + chr5.2:60317559-60317578 MS.gene53988:intron 30.0%
!!! GATTTTGGTTCAAAAGGAAT+AGG + chr5.2:60316392-60316411 MS.gene53988:intron 30.0%
!!! TTTGTTGCTTTTCATACCTT+GGG - chr5.2:60315570-60315589 None:intergenic 30.0%
!!! TTTTAAGGTAATGTAGTGTG+TGG + chr5.2:60315759-60315778 MS.gene53988:intron 30.0%
!!! TTTTGTTGCTTTTCATACCT+TGG - chr5.2:60315571-60315590 None:intergenic 30.0%
AAAATATGTCAGCCTAGCTA+GGG + chr5.2:60317648-60317667 MS.gene53988:intron 35.0%
AATGAATGGAATTCAGCTGT+TGG + chr5.2:60318675-60318694 MS.gene53988:CDS 35.0%
AATTCACCACTGTAAATGGA+AGG - chr5.2:60318900-60318919 None:intergenic 35.0%
ACCAATTGTGTCTCTACATT+AGG - chr5.2:60315942-60315961 None:intergenic 35.0%
ACCTAATGTAGAGACACAAT+TGG + chr5.2:60315938-60315957 MS.gene53988:CDS 35.0%
AGGTGGGATTATGTAGAATT+AGG + chr5.2:60317069-60317088 MS.gene53988:intron 35.0%
AGTCCTTACTCATAATGTGT+GGG - chr5.2:60317219-60317238 None:intergenic 35.0%
ATATGGTATATGCAGGACTA+TGG + chr5.2:60316750-60316769 MS.gene53988:intron 35.0%
ATGGAAGAATGTTGATGTGT+TGG + chr5.2:60316875-60316894 MS.gene53988:CDS 35.0%
ATGTAGAGACACAATTGGTT+AGG + chr5.2:60315943-60315962 MS.gene53988:CDS 35.0%
ATTCACCACTGTAAATGGAA+GGG - chr5.2:60318899-60318918 None:intergenic 35.0%
ATTCGTTAGGAAGGAATCAA+TGG + chr5.2:60315883-60315902 MS.gene53988:CDS 35.0%
CAGGGCCTAATAATTAAAAC+AGG + chr5.2:60318509-60318528 MS.gene53988:intron 35.0%
CATGATATCACAAAGAGGTA+GGG + chr5.2:60316486-60316505 MS.gene53988:intron 35.0%
CTGATTACCTTAAATACCGT+TGG + chr5.2:60315524-60315543 MS.gene53988:CDS 35.0%
GGAAAAACTGTGATGTTTGT+TGG + chr5.2:60315858-60315877 MS.gene53988:CDS 35.0%
GGATAAACACTAGAGAATGT+TGG + chr5.2:60318279-60318298 MS.gene53988:intron 35.0%
GGATATGATATGGTATATGC+AGG + chr5.2:60316743-60316762 MS.gene53988:intron 35.0%
GGGATTATGTAGAATTAGGA+GGG + chr5.2:60317073-60317092 MS.gene53988:CDS 35.0%
GGTTCTACTTCTGTCAATAT+TGG - chr5.2:60317172-60317191 None:intergenic 35.0%
TCATGATATCACAAAGAGGT+AGG + chr5.2:60316485-60316504 MS.gene53988:intron 35.0%
TGGGATTATGTAGAATTAGG+AGG + chr5.2:60317072-60317091 MS.gene53988:CDS 35.0%
TGTAGAGACACAATTGGTTA+GGG + chr5.2:60315944-60315963 MS.gene53988:CDS 35.0%
TTGAGTATGACCAAATTGGA+TGG - chr5.2:60317859-60317878 None:intergenic 35.0%
TTGTGTAATGGTAGGTTCAA+TGG + chr5.2:60315810-60315829 MS.gene53988:intron 35.0%
TTTAATACTGCTGCATTCTC+AGG - chr5.2:60317817-60317836 None:intergenic 35.0%
! AGGGAATTTTTACCCTGATA+TGG + chr5.2:60317092-60317111 MS.gene53988:CDS 35.0%
! CTTATTTGCATGAGCTTTTC+AGG + chr5.2:60318348-60318367 MS.gene53988:intron 35.0%
! GAATGATGGTGTTTGTGTAA+TGG + chr5.2:60315798-60315817 MS.gene53988:intron 35.0%
! GGAAAGAGGTATGAAAACAT+GGG + chr5.2:60317131-60317150 MS.gene53988:CDS 35.0%
! GTATGAAAACATGGGCTAAT+TGG + chr5.2:60317139-60317158 MS.gene53988:CDS 35.0%
! GTCTCAAAATTCTTTTCCCT+TGG - chr5.2:60316827-60316846 None:intergenic 35.0%
! TGGAAAGAGGTATGAAAACA+TGG + chr5.2:60317130-60317149 MS.gene53988:CDS 35.0%
! TTTGTTGATTGATTGAGTGC+AGG + chr5.2:60317049-60317068 MS.gene53988:intron 35.0%
!! ATGTTTGTTGGTGATTCGTT+AGG + chr5.2:60315870-60315889 MS.gene53988:CDS 35.0%
!! TAGAACCCATGTGTTATTTC+AGG + chr5.2:60317185-60317204 MS.gene53988:CDS 35.0%
!! TGATATGTAATAAGGGCGTT+TGG + chr5.2:60318240-60318259 MS.gene53988:intron 35.0%
AAGAATGTGAAAGTGGAGAG+AGG - chr5.2:60315975-60315994 None:intergenic 40.0%
ACGACAATGATACATGACAC+CGG - chr5.2:60316204-60316223 None:intergenic 40.0%
ACGACGACAAAGAATTGCTA+TGG + chr5.2:60318714-60318733 MS.gene53988:CDS 40.0%
ACTCACCAAGAATGTGAAAG+TGG - chr5.2:60315982-60316001 None:intergenic 40.0%
AGACACCAGAACTACAATCA+AGG - chr5.2:60316113-60316132 None:intergenic 40.0%
AGCGAGAACTTTGGTTAAGT+TGG - chr5.2:60317783-60317802 None:intergenic 40.0%
AGTCATCAAGGATCACTTCA+AGG + chr5.2:60316921-60316940 MS.gene53988:CDS 40.0%
CACAATTGAGTGGTTTCCAA+CGG - chr5.2:60315543-60315562 None:intergenic 40.0%
CAGATAAGAATGGTGACACA+TGG + chr5.2:60316856-60316875 MS.gene53988:CDS 40.0%
CCAACAAAAAACTTGGGTAG+TGG - chr5.2:60316363-60316382 None:intergenic 40.0%
CTACCAAACGATCCATATCA+GGG - chr5.2:60317107-60317126 None:intergenic 40.0%
CTTGATGAGGCAGATAAGAA+TGG + chr5.2:60316846-60316865 MS.gene53988:CDS 40.0%
GAAAATATGTCAGCCTAGCT+AGG + chr5.2:60317647-60317666 MS.gene53988:intron 40.0%
GAGTCCTTACTCATAATGTG+TGG - chr5.2:60317220-60317239 None:intergenic 40.0%
GATGGTGTTTGTGTAATGGT+AGG + chr5.2:60315802-60315821 MS.gene53988:intron 40.0%
GCATGAACACATCTGATCAA+AGG + chr5.2:60315600-60315619 MS.gene53988:intron 40.0%
GCGGTCAAATGTATATGATG+CGG + chr5.2:60316087-60316106 MS.gene53988:intron 40.0%
GCTCATCAGATGAATGCAAA+TGG - chr5.2:60317457-60317476 None:intergenic 40.0%
GTAGAGACACAATTGGTTAG+GGG + chr5.2:60315945-60315964 MS.gene53988:CDS 40.0%
GTCATCAAGGATCACTTCAA+GGG + chr5.2:60316922-60316941 MS.gene53988:CDS 40.0%
GTGATGTCAAGAAGATATGC+AGG - chr5.2:60318846-60318865 None:intergenic 40.0%
GTGGTTTCCAACGGTATTTA+AGG - chr5.2:60315534-60315553 None:intergenic 40.0%
TAACTTCGAAGCGAGAACTT+TGG - chr5.2:60317792-60317811 None:intergenic 40.0%
TAGATGTTGATGTGGTCCAA+GGG + chr5.2:60316808-60316827 MS.gene53988:CDS 40.0%
TCTCCCACACATTATGAGTA+AGG + chr5.2:60317213-60317232 MS.gene53988:intron 40.0%
TCTCTCCACTTTCACATTCT+TGG + chr5.2:60315974-60315993 MS.gene53988:CDS 40.0%
TTACCCTGATATGGATCGTT+TGG + chr5.2:60317101-60317120 MS.gene53988:CDS 40.0%
TTGATTGATTGAGTGCAGGT+GGG + chr5.2:60317053-60317072 MS.gene53988:intron 40.0%
! AAACTTGGGTAGTGGCAATA+TGG - chr5.2:60316355-60316374 None:intergenic 40.0%
! AGCAGTTGATTCCAAGTATC+AGG - chr5.2:60318990-60319009 None:intergenic 40.0%
! TATGGATCGTTTGGTAGCTT+TGG + chr5.2:60317110-60317129 MS.gene53988:CDS 40.0%
! TTTTCATTGGATCCCTAGCT+AGG - chr5.2:60317663-60317682 None:intergenic 40.0%
!! TTGTTGGTGATTCGTTAGGA+AGG + chr5.2:60315874-60315893 MS.gene53988:CDS 40.0%
!!! CCACTACCCAAGTTTTTTGT+TGG + chr5.2:60316360-60316379 MS.gene53988:intron 40.0%
AAGCTCTGCACATTGAAGTG+CGG - chr5.2:60317623-60317642 None:intergenic 45.0%
AGTGGCACAACAACAGACTT+TGG + chr5.2:60318753-60318772 MS.gene53988:CDS 45.0%
CAATGGCTACAATCAGCTGA+GGG - chr5.2:60318951-60318970 None:intergenic 45.0%
CATAATCTGATGAGCCAAGG+TGG + chr5.2:60318107-60318126 MS.gene53988:intron 45.0%
CATGTGTGATATTGCCACCT+TGG - chr5.2:60318124-60318143 None:intergenic 45.0%
CTGCTTCTAGATCTGGAAGT+GGG + chr5.2:60317476-60317495 MS.gene53988:intron 45.0%
GAGCATAATCTGATGAGCCA+AGG + chr5.2:60318104-60318123 MS.gene53988:intron 45.0%
GCTACCAAACGATCCATATC+AGG - chr5.2:60317108-60317127 None:intergenic 45.0%
GTAGATGTTGATGTGGTCCA+AGG + chr5.2:60316807-60316826 MS.gene53988:CDS 45.0%
GTTGATTGATTGAGTGCAGG+TGG + chr5.2:60317052-60317071 MS.gene53988:intron 45.0%
TACAATCAGCTGAGGGATCA+GGG - chr5.2:60318944-60318963 None:intergenic 45.0%
TCAAGGATCACTTCAAGGGT+AGG + chr5.2:60316926-60316945 MS.gene53988:intron 45.0%
TGATGAGCTGCTTCTAGATC+TGG + chr5.2:60317469-60317488 MS.gene53988:intron 45.0%
TGGTGTTCAACACAGGACAT+TGG + chr5.2:60316895-60316914 MS.gene53988:CDS 45.0%
TGTTCAACACAGGACATTGG+TGG + chr5.2:60316898-60316917 MS.gene53988:CDS 45.0%
TGTTTGCGTAGATGCTTCGT+AGG + chr5.2:60316237-60316256 MS.gene53988:intron 45.0%
TTCAAGTGTCTGTGTCGTGT+CGG + chr5.2:60315695-60315714 MS.gene53988:intron 45.0%
TTCCAAGTATCAGGCAAACC+AGG - chr5.2:60318981-60319000 None:intergenic 45.0%
! ACAACAACAGACTTTGGAGG+AGG + chr5.2:60318759-60318778 MS.gene53988:CDS 45.0%
! GAAAACATGGGCTAATTGGG+TGG + chr5.2:60317143-60317162 MS.gene53988:CDS 45.0%
! GATGTGTTGGTGTTCAACAC+AGG + chr5.2:60316888-60316907 MS.gene53988:CDS 45.0%
! TCTGTTGTTGTGCCACTGAT+TGG - chr5.2:60318750-60318769 None:intergenic 45.0%
! TTCCTGGTTTGCCTGATACT+TGG + chr5.2:60318976-60318995 MS.gene53988:CDS 45.0%
!! CGTTTGGTAGCTTTGGAAAG+AGG + chr5.2:60317117-60317136 MS.gene53988:CDS 45.0%
ATCAGGGTCAGCTTTCTGCT+GGG - chr5.2:60318928-60318947 None:intergenic 50.0%
CACTCAATTGTGAGCTCCCA+AGG + chr5.2:60315551-60315570 MS.gene53988:CDS 50.0%
CCAATGGCTACAATCAGCTG+AGG - chr5.2:60318952-60318971 None:intergenic 50.0%
CCTCAGCTGATTGTAGCCAT+TGG + chr5.2:60318949-60318968 MS.gene53988:CDS 50.0%
CTACAATCAGCTGAGGGATC+AGG - chr5.2:60318945-60318964 None:intergenic 50.0%
CTTGGGAGCTCACAATTGAG+TGG - chr5.2:60315553-60315572 None:intergenic 50.0%
GCAAACCAGGAAGACACCAA+TGG - chr5.2:60318968-60318987 None:intergenic 50.0%
GCTGCTTCTAGATCTGGAAG+TGG + chr5.2:60317475-60317494 MS.gene53988:intron 50.0%
GGACACCCTTCCATTTACAG+TGG + chr5.2:60318891-60318910 MS.gene53988:CDS 50.0%
GGACATTGGTGGAGTCATCA+AGG + chr5.2:60316909-60316928 MS.gene53988:CDS 50.0%
GGCACAACAACAGACTTTGG+AGG + chr5.2:60318756-60318775 MS.gene53988:CDS 50.0%
GGTGAAACAGCACCAATCAG+TGG + chr5.2:60318735-60318754 MS.gene53988:CDS 50.0%
TGCGGCCTTGATTGTAGTTC+TGG + chr5.2:60316105-60316124 MS.gene53988:intron 50.0%
! CATCTGATCAAAGGCGTGTC+TGG + chr5.2:60315609-60315628 MS.gene53988:intron 50.0%
! TGTAGCCATTGGTGTCTTCC+TGG + chr5.2:60318960-60318979 MS.gene53988:CDS 50.0%
GATCAGGGTCAGCTTTCTGC+TGG - chr5.2:60318929-60318948 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr5.2 gene 60315505 60319031 60315505 ID=MS.gene53988
chr5.2 mRNA 60315505 60319031 60315505 ID=MS.gene53988.t1;Parent=MS.gene53988
chr5.2 exon 60315505 60315572 60315505 ID=MS.gene53988.t1.exon1;Parent=MS.gene53988.t1
chr5.2 CDS 60315505 60315572 60315505 ID=cds.MS.gene53988.t1;Parent=MS.gene53988.t1
chr5.2 exon 60315824 60315995 60315824 ID=MS.gene53988.t1.exon2;Parent=MS.gene53988.t1
chr5.2 CDS 60315824 60315995 60315824 ID=cds.MS.gene53988.t1;Parent=MS.gene53988.t1
chr5.2 exon 60316765 60316943 60316765 ID=MS.gene53988.t1.exon3;Parent=MS.gene53988.t1
chr5.2 CDS 60316765 60316943 60316765 ID=cds.MS.gene53988.t1;Parent=MS.gene53988.t1
chr5.2 exon 60317071 60317229 60317071 ID=MS.gene53988.t1.exon4;Parent=MS.gene53988.t1
chr5.2 CDS 60317071 60317229 60317071 ID=cds.MS.gene53988.t1;Parent=MS.gene53988.t1
chr5.2 exon 60318671 60319031 60318671 ID=MS.gene53988.t1.exon5;Parent=MS.gene53988.t1
chr5.2 CDS 60318671 60319031 60318671 ID=cds.MS.gene53988.t1;Parent=MS.gene53988.t1
Gene Sequence

>MS.gene53988

ATGAATGGTCGTCCTGATTCTGATTACCTTAAATACCGTTGGAAACCACTCAATTGTGAGCTCCCAAGGTATGAAAAGCAACAAAAGAATATGTAGCATGAACACATCTGATCAAAGGCGTGTCTGGTGTTTGACACAAGTTCACGCTAACACGACACCTACAACTTGATTATGTTCAATAATTAATTTTTTCAAGTGTCTGTGTCGTGTCGGTGTTCGTGTTTGTGTCCGTGCTTTAAAGCAAAATATTGTGTTTTTAAGGTAATGTAGTGTGTGGATGCTTAGCATAATTTGAATGATGGTGTTTGTGTAATGGTAGGTTCAATGGAGTTGAGTTTTTGTTGAAGATGAAAGGAAAAACTGTGATGTTTGTTGGTGATTCGTTAGGAAGGAATCAATGGCAGTCTTTGATTTGTATGATATCTGCTGCAGCACCTAATGTAGAGACACAATTGGTTAGGGGAGATCCTCTCTCCACTTTCACATTCTTGGTGAGTCTAGAAAAAACTTCTGAGCTTTAAGTTGATTGCTAACAAAAAGTTTTAAATTGTGGTTGTGATCATTGATATATTCGGTAGAATTGCGGTCAAATGTATATGATGCGGCCTTGATTGTAGTTCTGGTGTCTGACATGTGCTAGTATCTGACACTAACACTTGTGATTACATTCAATTATATCATTTTTTCAAATTATTACCGGTGTCATGTATCATTGTCGTGTTTGTGTGACTGTGTTTGCGTAGATGCTTCGTAGGTATAGATAAAACAAAATACTTTGTTGATAACTATCAAATACCAATACCTCATTTTGCATTTCACACTATTTCTATTGCTAGTAAGATTTAAACCATATTGCCACTACCCAAGTTTTTTGTTGGTAAGATTTAGATTTTGGTTCAAAAGGAATAGGTAGATAAGAACTAATAACTATGCTAATTTCCAGCAATAAAATAGTTGTTTCCCTCTTTCTAAAGTTTCATTCATGATATCACAAAGAGGTAGGGTCATATTTTTTTTGGTATAAGACAAAGAAGCTGTTGAAAAAGTGTTTGTAAAACAGTGTCAATTGAATCATTAGATAGATATAAGGGTCCAATTAAATAATATGCATATGAGGGGCCCCAAAATATTTTCAAGAATTTTGATACATGGTTACATACTGCATACATCATTTCTATTATTTATCCTGTAGAATATTGACTATGCAAACAAGAAAATATTTTAGTGATGTTAAAATTGGATATGATATGGTATATGCAGGACTATGGTGTGAAAATTTCATTTCATAGAACTCCTTATTTAGTAGATGTTGATGTGGTCCAAGGGAAAAGAATTTTGAGACTTGATGAGGCAGATAAGAATGGTGACACATGGAAGAATGTTGATGTGTTGGTGTTCAACACAGGACATTGGTGGAGTCATCAAGGATCACTTCAAGGGTAGGCAAGAATAGTATCTTCTCTTTTTTAAGCTTATTCAAGAAAACACTATAAGAAAATGACTTTTTGAATTTGTACTTAAAAAAGGTTGATTTTTTGGGCTGATTTGTTGATTGATTGAGTGCAGGTGGGATTATGTAGAATTAGGAGGGAATTTTTACCCTGATATGGATCGTTTGGTAGCTTTGGAAAGAGGTATGAAAACATGGGCTAATTGGGTGGATGCCAATATTGACAGAAGTAGAACCCATGTGTTATTTCAGGCAATTTCTCCCACACATTATGAGTAAGGACTCTTCTATATGTCTCTATTTTGAACCAATTATGAATAATTGATTCTTTTTTACTAAGCTGTAATTGATTATTTGTAACTTGAAAATAGGGCAGTCATTATTAATTTAGTGATTTAAAAATGGTTAAATTTAACAAGTTACTATAATTAATCAGACACTTGAAGCAAGATGCCAAATTAACTTTTTCCCTAAAGTCAAACTAAATTTACATTTTTTTCCATTTGCATTCATCTGATGAGCTGCTTCTAGATCTGGAAGTGGGACCTTTCTTTTGACTATTCTTGACAAGTATGCCCATATCTTGTTGTAATTTGATTATTCTATTCTGAGGCTGAAATTTTTTGGCTTGCTTGTTGGTGCCATCTTGTCACAGAATTATGAATCCGCACTTCAATGTGCAGAGCTTACAAGAAAATATGTCAGCCTAGCTAGGGATCCAATGAAAACACATAAGAAAGATAAAGAATTAGGAAGAAAAAATGGTTGTGATAAGAAACCTTTAAATCATGCAACAACACATGGATGAGATAGTTTTAGACTCTTCCAACTTAACCAAAGTTCTCGCTTCGAAGTTAGACCTGAGAATGCAGCAGTATTAAACCTCTTAGAAAGAGTTTTACCATCCAATTTGGTCATACTCAACTAAAGAGATTCGTCTTTGTAGTTGCTTGCATATGATACCCGTTTTATATTGAGGAAAATATTTTTTAATGATTTTTTAGTATAAACAAATATAGTAAATAAATTGGTTAGACAAGAAAGTATGCTGAATAAATTTCTAGGTAATAAAATTACATATTTTTACTAGCATTAATACGACATTCTTACTAAAGAAAACAAATTATCAAAACTATGTCATAATCTGATGAGCATAATCTGATGAGCCAAGGTGGCAATATCACACATGAATATGTTCAGTTATGTGATATTGAATGAAAGATTCAGCCCAATCGAGTATAATGAAAAAAAATTGCTTCTTTAGCTTTTTTTAGAATACAAGCATTGATATGTAATAAGGGCGTTTGGATTCATTTATTTACTTGGATAAACACTAGAGAATGTTGGAAGAGCTTATGAAACAACTTATTACATTCCCATAAGTTGTTTTTAGCTTATTTGCATGAGCTTTTCAGGTTTATGACTGCAAAAGTTTATAGTTTATATGTAAACTGTTTGCTTTGTTTTGTATTTCCCAATAGAAATAATTTATACATAAGCACTTATCATGTAAGTGCTTATGCTATAAGTGCATAATTAAATTGTTTATTCAAACAGGGCCTAATAATTAAAACAGGAATTAGTGCTTTTCAAATTTGACAGTAAAAATGTAACATGGCTTTATTACATTAATATTTGGTTAAGCTAGTATGTATATTTTTTAAACAAAAGTTATGCTAGGATTTGGTTGCACTGAACACTGATATTTATATGTAGCAAAAATGAATGGAATTCAGCTGTTGGTAGAGCAACAAGTGCAACGACGACAAAGAATTGCTATGGTGAAACAGCACCAATCAGTGGCACAACAACAGACTTTGGAGGAGGAGAAACATACACTGATCAACAAATGAGAGTAGTTAATATGGTGATTCGAGAAATGCGCGATCCTGCATATCTTCTTGACATCACAATGTTATCAGAAATGAGGAAAGATGGACACCCTTCCATTTACAGTGGTGAATTTAGTTCCCAGCAGAAAGCTGACCCTGATCCCTCAGCTGATTGTAGCCATTGGTGTCTTCCTGGTTTGCCTGATACTTGGAATCAACTGCTCTATGTTGCTTTATTCTATTGA
Protein sequence

>MS.gene53988.t1

MNGRPDSDYLKYRWKPLNCELPRFNGVEFLLKMKGKTVMFVGDSLGRNQWQSLICMISAAAPNVETQLVRGDPLSTFTFLDYGVKISFHRTPYLVDVDVVQGKRILRLDEADKNGDTWKNVDVLVFNTGHWWSHQGSLQGWDYVELGGNFYPDMDRLVALERGMKTWANWVDANIDRSRTHVLFQAISPTHYDKNEWNSAVGRATSATTTKNCYGETAPISGTTTDFGGGETYTDQQMRVVNMVIREMRDPAYLLDITMLSEMRKDGHPSIYSGEFSSQQKADPDPSADCSHWCLPGLPDTWNQLLYVALFY