Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54999.t1 | XP_003624422.2 | 96.1 | 415 | 16 | 0 | 1 | 415 | 1 | 415 | 3.60E-235 | 823.9 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54999.t1 | Q94K73 | 75.5 | 413 | 96 | 2 | 4 | 415 | 8 | 416 | 5.3e-187 | 655.2 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54999.t1 | G7KW40 | 96.1 | 415 | 16 | 0 | 1 | 415 | 1 | 415 | 2.6e-235 | 823.9 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene057207 | MS.gene54999 | 0.807893 | 4.05E-50 | -1.69E-46 |
| MS.gene05886 | MS.gene54999 | 0.803753 | 3.00E-49 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54999.t1 | MTR_7g083030 | 96.145 | 415 | 16 | 0 | 1 | 415 | 1 | 415 | 0.0 | 809 |
| MS.gene54999.t1 | MTR_7g083030 | 96.791 | 374 | 12 | 0 | 1 | 374 | 1 | 374 | 0.0 | 759 |
| MS.gene54999.t1 | MTR_8g046320 | 63.333 | 60 | 16 | 1 | 71 | 130 | 96 | 149 | 7.15e-15 | 72.8 |
| MS.gene54999.t1 | MTR_4g070040 | 24.373 | 279 | 147 | 9 | 82 | 312 | 275 | 537 | 1.49e-11 | 67.0 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene54999.t1 | AT3G58140 | 79.474 | 380 | 77 | 1 | 37 | 415 | 37 | 416 | 0.0 | 650 |
Find 109 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAAAGTGGTTGGAGGTTT+TGG | 0.167441 | 7.2:+24311360 | MS.gene54999:intron |
| AGCAAGTTCAACACACTTTA+TGG | 0.176559 | 7.2:+24319053 | MS.gene54999:CDS |
| TCAAATGATGGATTAGTAAA+TGG | 0.178094 | 7.2:-24310113 | None:intergenic |
| AATAATGTGGCTTGGGCTTT+TGG | 0.185517 | 7.2:+24311430 | MS.gene54999:CDS |
| CATGTAGATGGAAGGCTTTC+GGG | 0.192058 | 7.2:+24309238 | MS.gene54999:intron |
| GCTACAAGGACATCAGTTTC+TGG | 0.241259 | 7.2:+24312505 | MS.gene54999:CDS |
| GGTCTAGCACGCCATTTATT+TGG | 0.274438 | 7.2:+24309341 | MS.gene54999:CDS |
| CAGACTCACTTCCTTGTTAC+TGG | 0.290649 | 7.2:+24309092 | MS.gene54999:CDS |
| ATTTGCAAATACCAAATAAA+TGG | 0.292920 | 7.2:-24309352 | None:intergenic |
| TACCGGATATTCGTCTCTTT+TGG | 0.305942 | 7.2:+24311491 | MS.gene54999:CDS |
| CAGTTCTCCAAGGGCCAGTT+AGG | 0.327821 | 7.2:+24311713 | MS.gene54999:intron |
| TCATGTAGATGGAAGGCTTT+CGG | 0.328640 | 7.2:+24309237 | MS.gene54999:intron |
| TGGATTAGTAAATGGGAAAT+AGG | 0.328651 | 7.2:-24310105 | None:intergenic |
| CAAGAGATTCTGAAAAGAAA+TGG | 0.334132 | 7.2:+24311400 | MS.gene54999:CDS |
| CTCCTACTCACATGGTCTTC+AGG | 0.337485 | 7.2:-24308984 | None:intergenic |
| GCTTAAATTTGATCCCTAAC+TGG | 0.360988 | 7.2:-24311727 | None:intergenic |
| AATTTGTGTGAAGTTGTTAG+AGG | 0.365693 | 7.2:+24312552 | MS.gene54999:CDS |
| GCAAGTTCAACACACTTTAT+GGG | 0.370438 | 7.2:+24319054 | MS.gene54999:CDS |
| GCCTCGATTTCTTGCGAGAA+AGG | 0.380972 | 7.2:-24307424 | None:intergenic |
| TGAACTTGAAATATATTTCA+AGG | 0.384152 | 7.2:+24310133 | MS.gene54999:CDS |
| TAGGATTGCATATAGATCTA+TGG | 0.385783 | 7.2:+24314741 | MS.gene54999:CDS |
| GTCAAAGTAATAATAGTCTA+AGG | 0.392541 | 7.2:+24307345 | MS.gene54999:CDS |
| CAAATGATGGATTAGTAAAT+GGG | 0.404320 | 7.2:-24310112 | None:intergenic |
| GTGCCACACAAGTGCTCATC+AGG | 0.405655 | 7.2:+24309049 | MS.gene54999:CDS |
| GCTAGTCATTCCTTTCTTGT+TGG | 0.408903 | 7.2:-24314710 | None:intergenic |
| TATATTTCAAGTTCAAATGA+TGG | 0.411951 | 7.2:-24310125 | None:intergenic |
| CCAGACGAATGGGAAGCATC+TGG | 0.417468 | 7.2:+24309269 | MS.gene54999:CDS |
| TCCCATTGTTTCACTAAAGC+AGG | 0.417601 | 7.2:+24308331 | MS.gene54999:CDS |
| GAATGACTAGCCACTGCTAT+AGG | 0.420827 | 7.2:+24314722 | MS.gene54999:CDS |
| ATGAGTTCAACAAGAGAAAC+CGG | 0.422576 | 7.2:-24319029 | None:intergenic |
| CCAGAACTTTGATGATGTCT+TGG | 0.433665 | 7.2:+24308959 | MS.gene54999:intron |
| GAAATCTAATAATGTGGCTT+GGG | 0.441045 | 7.2:+24311423 | MS.gene54999:CDS |
| GTAGAAGTTACCTTTGAAAA+CGG | 0.443632 | 7.2:-24311749 | None:intergenic |
| AGGAGGGATCAACACCCTCT+TGG | 0.447438 | 7.2:+24308233 | MS.gene54999:CDS |
| AACTCATCGGCCAGGTGTCC+CGG | 0.447936 | 7.2:+24319010 | MS.gene54999:CDS |
| GGAAATCTAATAATGTGGCT+TGG | 0.453728 | 7.2:+24311422 | MS.gene54999:CDS |
| CCAGATGCTTCCCATTCGTC+TGG | 0.454560 | 7.2:-24309269 | None:intergenic |
| AAGAGATTCTGAAAAGAAAT+GGG | 0.459133 | 7.2:+24311401 | MS.gene54999:CDS |
| AACTTGGAATGCAGCTTCAT+AGG | 0.462486 | 7.2:+24308213 | MS.gene54999:CDS |
| CAAAGAGGAGTCGGAGGAGG+AGG | 0.463212 | 7.2:-24318963 | None:intergenic |
| GACAATTTCACCAACAAGAA+AGG | 0.464674 | 7.2:+24314700 | MS.gene54999:CDS |
| GGAGGGATCAACACCCTCTT+GGG | 0.471379 | 7.2:+24308234 | MS.gene54999:CDS |
| TGGCACCTTAAAACAGTTTG+AGG | 0.471696 | 7.2:-24309032 | None:intergenic |
| GAAGTTGTTAGAGGAGTAGC+GGG | 0.474332 | 7.2:+24312561 | MS.gene54999:CDS |
| GTTCACTCTGCAGTGGAGAT+TGG | 0.476372 | 7.2:+24307455 | MS.gene54999:CDS |
| CAGATTGTTAATTTCATCAT+CGG | 0.476616 | 7.2:-24314776 | None:intergenic |
| AGGTTTGGAGAGGTTAGCTA+TGG | 0.487707 | 7.2:+24311456 | MS.gene54999:CDS |
| GACCAAAAGAGACGAATATC+CGG | 0.488025 | 7.2:-24311493 | None:intergenic |
| TTTGATCCCTAACTGGCCCT+TGG | 0.490169 | 7.2:-24311720 | None:intergenic |
| GTCTCAGGAAAAGTGGTTGG+AGG | 0.505575 | 7.2:+24311354 | MS.gene54999:intron |
| GGACATACTTGATGGAAGAC+AGG | 0.511865 | 7.2:-24309155 | None:intergenic |
| CATTGTACTTACAGGTGATG+TGG | 0.514483 | 7.2:+24310064 | MS.gene54999:intron |
| CTGTCTGGAACATTATTTGT+TGG | 0.517372 | 7.2:-24308179 | None:intergenic |
| TGGGAAGCATCTGGGATGGA+TGG | 0.519117 | 7.2:+24309278 | MS.gene54999:CDS |
| ACAAGAGAAACCGGGACACC+TGG | 0.520980 | 7.2:-24319020 | None:intergenic |
| TGATGAAATTAACAATCTGC+AGG | 0.522651 | 7.2:+24314780 | MS.gene54999:CDS |
| TACTGGAGATGTTTATCGAA+GGG | 0.523038 | 7.2:+24309109 | MS.gene54999:CDS |
| TATGGTTCTATTTGACATAC+CGG | 0.523107 | 7.2:+24311474 | MS.gene54999:CDS |
| TGAAGTTGTTAGAGGAGTAG+CGG | 0.528932 | 7.2:+24312560 | MS.gene54999:CDS |
| TTTGATATCATGTAGATGGA+AGG | 0.529389 | 7.2:+24309230 | MS.gene54999:intron |
| TTTCTTGCGAGAAAGGTTGT+TGG | 0.533020 | 7.2:-24307417 | None:intergenic |
| CATTATTGAACTCATCGGCC+AGG | 0.533679 | 7.2:+24319002 | MS.gene54999:CDS |
| AGAAGCGAAGTACCTTGTCT+TGG | 0.533750 | 7.2:-24307491 | None:intergenic |
| CAGACGAATGGGAAGCATCT+GGG | 0.538873 | 7.2:+24309270 | MS.gene54999:CDS |
| TTTGTCTCAGGAAAAGTGGT+TGG | 0.539106 | 7.2:+24311351 | MS.gene54999:intron |
| CAGACCTAAAGAATTGCCTC+GGG | 0.540838 | 7.2:+24309318 | MS.gene54999:CDS |
| ACCTGCTTTAGTGAAACAAT+GGG | 0.540880 | 7.2:-24308332 | None:intergenic |
| GCAGACCTAAAGAATTGCCT+CGG | 0.544655 | 7.2:+24309317 | MS.gene54999:CDS |
| AGTTCTCCAAGGGCCAGTTA+GGG | 0.546188 | 7.2:+24311714 | MS.gene54999:intron |
| GTTCAAAGAGGAGTCGGAGG+AGG | 0.546395 | 7.2:-24318966 | None:intergenic |
| TGATTAGTTCAAAGAGGAGT+CGG | 0.547401 | 7.2:-24318972 | None:intergenic |
| CAGGTGATGTGGAAATGCGC+TGG | 0.552606 | 7.2:+24310075 | MS.gene54999:intron |
| GGCACCTTAAAACAGTTTGA+GGG | 0.554011 | 7.2:-24309031 | None:intergenic |
| TTGGAATGCAGCTTCATAGG+AGG | 0.555950 | 7.2:+24308216 | MS.gene54999:CDS |
| CACTCTGCAGTGGAGATTGG+TGG | 0.556082 | 7.2:+24307458 | MS.gene54999:CDS |
| TTAGTGAAACAATGGGAGAA+AGG | 0.557845 | 7.2:-24308325 | None:intergenic |
| CATTCTTGATTATCCCAAGA+GGG | 0.562797 | 7.2:-24308247 | None:intergenic |
| TAGCGAAAGTGTAGCATGCA+CGG | 0.566388 | 7.2:-24307318 | None:intergenic |
| CAGGCAGAGTTATTGAGAGA+TGG | 0.574894 | 7.2:+24309068 | MS.gene54999:CDS |
| TACCTGCTTTAGTGAAACAA+TGG | 0.575321 | 7.2:-24308333 | None:intergenic |
| ACAGTATCCTCCTTGCTACA+AGG | 0.579898 | 7.2:+24312491 | MS.gene54999:intron |
| AGCGGGAGATCTTGTTGAAG+AGG | 0.582951 | 7.2:+24312578 | MS.gene54999:CDS |
| AAGTTCATTATTGAACTCAT+CGG | 0.583092 | 7.2:+24318997 | MS.gene54999:CDS |
| TTACTGGAGATGTTTATCGA+AGG | 0.593612 | 7.2:+24309108 | MS.gene54999:CDS |
| CGAATGGGAAGCATCTGGGA+TGG | 0.598134 | 7.2:+24309274 | MS.gene54999:CDS |
| CATTATAGCTCCTACTCACA+TGG | 0.599944 | 7.2:-24308992 | None:intergenic |
| GAAGCAGCGGAAGATGAGAA+AGG | 0.601877 | 7.2:-24307386 | None:intergenic |
| ACTTGCTGATTAGTTCAAAG+AGG | 0.601982 | 7.2:-24318978 | None:intergenic |
| TGATGAGCGATTTACCTCCC+AGG | 0.602322 | 7.2:+24311519 | MS.gene54999:CDS |
| GTTGGCAGAGAGAGAAGCAG+CGG | 0.603802 | 7.2:-24307399 | None:intergenic |
| AAATGGGAAATCTAATAATG+TGG | 0.604431 | 7.2:+24311417 | MS.gene54999:CDS |
| ACCTTTCTCGCAAGAAATCG+AGG | 0.605184 | 7.2:+24307423 | MS.gene54999:CDS |
| GCTGCAAGTTCACTCTGCAG+TGG | 0.610263 | 7.2:+24307448 | MS.gene54999:CDS |
| TGGAATGCAGCTTCATAGGA+GGG | 0.619208 | 7.2:+24308217 | MS.gene54999:CDS |
| AAACTGATGTCCTTGTAGCA+AGG | 0.623794 | 7.2:-24312501 | None:intergenic |
| AGGTGATGTGGAAATGCGCT+GGG | 0.629002 | 7.2:+24310076 | MS.gene54999:intron |
| GATAAACATCTCCAGTAACA+AGG | 0.635298 | 7.2:-24309103 | None:intergenic |
| CTGCCTGATGAGCACTTGTG+TGG | 0.638074 | 7.2:-24309052 | None:intergenic |
| TTAGTTCAAAGAGGAGTCGG+AGG | 0.644547 | 7.2:-24318969 | None:intergenic |
| GACCTAAAGAATTGCCTCGG+GGG | 0.663667 | 7.2:+24309320 | MS.gene54999:CDS |
| GACATACTTGATGGAAGACA+GGG | 0.664877 | 7.2:-24309154 | None:intergenic |
| TATATGCAATCCTATAGCAG+TGG | 0.667775 | 7.2:-24314732 | None:intergenic |
| GCATTCTTGATTATCCCAAG+AGG | 0.675459 | 7.2:-24308248 | None:intergenic |
| TGAGTTCAACAAGAGAAACC+GGG | 0.680749 | 7.2:-24319028 | None:intergenic |
| TTCCTGAAGACCATGTGAGT+AGG | 0.684735 | 7.2:+24308982 | MS.gene54999:CDS |
| CTGATGTCCTTGTAGCAAGG+AGG | 0.690224 | 7.2:-24312498 | None:intergenic |
| GGAGTCAAGATTCCAAGACA+AGG | 0.707397 | 7.2:+24307479 | MS.gene54999:CDS |
| AGACCTAAAGAATTGCCTCG+GGG | 0.733992 | 7.2:+24309319 | MS.gene54999:CDS |
| ATGTTCAATAAATACCTGGG+AGG | 0.736871 | 7.2:-24311533 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24307305 | 24319083 | 24307305 | ID=MS.gene54999 |
| chr7.2 | mRNA | 24307305 | 24319083 | 24307305 | ID=MS.gene54999.t1;Parent=MS.gene54999 |
| chr7.2 | exon | 24307305 | 24307500 | 24307305 | ID=MS.gene54999.t1.exon1;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24307305 | 24307500 | 24307305 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24308159 | 24308352 | 24308159 | ID=MS.gene54999.t1.exon2;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24308159 | 24308352 | 24308159 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24308963 | 24309169 | 24308963 | ID=MS.gene54999.t1.exon3;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24308963 | 24309169 | 24308963 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24309245 | 24309362 | 24309245 | ID=MS.gene54999.t1.exon4;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24309245 | 24309362 | 24309245 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24310078 | 24310154 | 24310078 | ID=MS.gene54999.t1.exon5;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24310078 | 24310154 | 24310078 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24311361 | 24311540 | 24311361 | ID=MS.gene54999.t1.exon6;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24311361 | 24311540 | 24311361 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24311716 | 24311760 | 24311716 | ID=MS.gene54999.t1.exon7;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24311716 | 24311760 | 24311716 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24312495 | 24312599 | 24312495 | ID=MS.gene54999.t1.exon8;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24312495 | 24312599 | 24312495 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24314688 | 24314801 | 24314688 | ID=MS.gene54999.t1.exon9;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24314688 | 24314801 | 24314688 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| chr7.2 | exon | 24318955 | 24319083 | 24318955 | ID=MS.gene54999.t1.exon10;Parent=MS.gene54999.t1 |
| chr7.2 | CDS | 24318955 | 24319083 | 24318955 | ID=cds.MS.gene54999.t1;Parent=MS.gene54999.t1 |
| Gene Sequence |
| Protein sequence |
