Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55049.t1 | XP_003624346.2 | 87.3 | 189 | 21 | 2 | 1 | 188 | 46 | 232 | 1.80E-52 | 215.7 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55049.t1 | G7KUB4 | 87.3 | 189 | 21 | 2 | 1 | 188 | 1 | 187 | 1.3e-52 | 215.7 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene050310 | MS.gene55049 | 0.812042 | 5.17E-51 | -1.69E-46 |
| MS.gene051720 | MS.gene55049 | 0.818863 | 1.57E-52 | -1.69E-46 |
| MS.gene05894 | MS.gene55049 | 0.816817 | 4.55E-52 | -1.69E-46 |
| MS.gene060519 | MS.gene55049 | 0.827602 | 1.43E-54 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55049.t1 | MTR_7g082230 | 94.737 | 190 | 5 | 3 | 1 | 188 | 1 | 187 | 6.47e-119 | 334 |
| MS.gene55049.t1 | MTR_6g018780 | 52.027 | 148 | 66 | 3 | 34 | 178 | 302 | 447 | 2.46e-36 | 131 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55049.t1 | AT1G67035 | 40.571 | 175 | 76 | 9 | 3 | 166 | 38 | 195 | 1.42e-24 | 96.3 |
| MS.gene55049.t1 | AT5G38300 | 35.377 | 212 | 69 | 8 | 3 | 166 | 43 | 234 | 6.46e-18 | 79.3 |
| MS.gene55049.t1 | AT1G67035 | 38.636 | 132 | 64 | 6 | 3 | 123 | 38 | 163 | 4.14e-15 | 70.5 |
Find 34 sgRNAs with CRISPR-Local
Find 36 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTCATGCTAATGTTGCTTC+TGG | 0.275975 | 7.2:+24840307 | MS.gene55049:CDS |
| CGTAACATTGAAGAGAAATC+TGG | 0.289550 | 7.2:-24840364 | None:intergenic |
| TTTCTTGCTGTTGTTGTTGT+TGG | 0.294481 | 7.2:-24840231 | None:intergenic |
| GTTGGTATTGTTGTAGTCTT+AGG | 0.306868 | 7.2:-24840213 | None:intergenic |
| CCTTTGTTCTTGTTCTTCAA+TGG | 0.319089 | 7.2:-24840445 | None:intergenic |
| CCACAACAACACCATGGATA+TGG | 0.328554 | 7.2:+24840129 | None:intergenic |
| AGAAAGGGTGTTGGTGGTGG+TGG | 0.374544 | 7.2:+24840538 | MS.gene55049:CDS |
| TTTGCGTATGCATTGTATCT+TGG | 0.422318 | 7.2:-24840160 | None:intergenic |
| AATGTTGCTTCTGGAAGAAA+AGG | 0.427927 | 7.2:+24840316 | MS.gene55049:CDS |
| GGTAGGAATAGTAGAGTTGT+GGG | 0.458384 | 7.2:+24840646 | MS.gene55049:CDS |
| CTAGGTTGTGGTATATTCGT+TGG | 0.471126 | 7.2:-24840403 | None:intergenic |
| AAGAGAAAGGGTGTTGGTGG+TGG | 0.486531 | 7.2:+24840535 | MS.gene55049:CDS |
| TTGTTCTTGTTCTTCAATGG+AGG | 0.510516 | 7.2:-24840442 | None:intergenic |
| GGGTAGGAATAGTAGAGTTG+TGG | 0.521383 | 7.2:+24840645 | MS.gene55049:CDS |
| TGGTCCTCTCTCCATATCCA+TGG | 0.530913 | 7.2:-24840140 | None:intergenic |
| GAGAAGAGAAAGGGTGTTGG+TGG | 0.537972 | 7.2:+24840532 | MS.gene55049:CDS |
| TGCTGCTGCAGAGAAGAGAA+AGG | 0.551914 | 7.2:+24840522 | MS.gene55049:CDS |
| AACAAGAACAAAGGTTGTGT+TGG | 0.563783 | 7.2:+24840454 | MS.gene55049:CDS |
| AAGAACAAAGGTTGTGTTGG+TGG | 0.573658 | 7.2:+24840457 | MS.gene55049:CDS |
| ATGCTATCAACGGTGAGGGT+AGG | 0.579267 | 7.2:+24840629 | MS.gene55049:CDS |
| TGGTGGTGGTGGAATTCTGA+TGG | 0.590344 | 7.2:+24840549 | MS.gene55049:CDS |
| GCAAATGCTATCAACGGTGA+GGG | 0.600356 | 7.2:+24840625 | MS.gene55049:CDS |
| GTGGTGGAATTCTGATGGCA+AGG | 0.603777 | 7.2:+24840554 | MS.gene55049:CDS |
| AAAGTAGTAGCACTAGGTTG+TGG | 0.616412 | 7.2:-24840415 | None:intergenic |
| ATGTTGCTTCTGGAAGAAAA+GGG | 0.616791 | 7.2:+24840317 | MS.gene55049:CDS |
| GCTGCTGCAGAGAAGAGAAA+GGG | 0.619325 | 7.2:+24840523 | MS.gene55049:CDS |
| AACAAAGGTTGTGTTGGTGG+TGG | 0.630030 | 7.2:+24840460 | MS.gene55049:CDS |
| TGCAAATGCTATCAACGGTG+AGG | 0.637390 | 7.2:+24840624 | MS.gene55049:CDS |
| GGTGTCAAAGTAGTAGCACT+AGG | 0.642015 | 7.2:-24840421 | None:intergenic |
| GCTACTGCAAATGCTATCAA+CGG | 0.652642 | 7.2:+24840619 | MS.gene55049:CDS |
| AACACCATGGATATGGAGAG+AGG | 0.667075 | 7.2:+24840136 | None:intergenic |
| GCAGAGAAGAGAAAGGGTGT+TGG | 0.674549 | 7.2:+24840529 | MS.gene55049:CDS |
| GTAGGAATAGTAGAGTTGTG+GGG | 0.681971 | 7.2:+24840647 | MS.gene55049:CDS |
| CCATTGAAGAACAAGAACAA+AGG | 0.687599 | 7.2:+24840445 | MS.gene55049:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | TGAAACTTGACTTGTTTTGT+TGG | - | chr7.2:24840292-24840311 | None:intergenic | 30.0% |
| AACAAGAACAAAGGTTGTGT+TGG | + | chr7.2:24840454-24840473 | MS.gene55049:CDS | 35.0% | |
| CCATTGAAGAACAAGAACAA+AGG | + | chr7.2:24840445-24840464 | MS.gene55049:CDS | 35.0% | |
| CCTTTGTTCTTGTTCTTCAA+TGG | - | chr7.2:24840448-24840467 | None:intergenic | 35.0% | |
| CGTAACATTGAAGAGAAATC+TGG | - | chr7.2:24840367-24840386 | None:intergenic | 35.0% | |
| GTTGGTATTGTTGTAGTCTT+AGG | - | chr7.2:24840216-24840235 | None:intergenic | 35.0% | |
| TTGTTCTTGTTCTTCAATGG+AGG | - | chr7.2:24840445-24840464 | None:intergenic | 35.0% | |
| TTTGCGTATGCATTGTATCT+TGG | - | chr7.2:24840163-24840182 | None:intergenic | 35.0% | |
| ! | AATGTTGCTTCTGGAAGAAA+AGG | + | chr7.2:24840316-24840335 | MS.gene55049:CDS | 35.0% |
| ! | ATGTTGCTTCTGGAAGAAAA+GGG | + | chr7.2:24840317-24840336 | MS.gene55049:CDS | 35.0% |
| ! | GTGTTTTGTCAAGTTCAAGA+GGG | + | chr7.2:24840482-24840501 | MS.gene55049:CDS | 35.0% |
| ! | TTTCATGCTAATGTTGCTTC+TGG | + | chr7.2:24840307-24840326 | MS.gene55049:CDS | 35.0% |
| !! | TTTCTTGCTGTTGTTGTTGT+TGG | - | chr7.2:24840234-24840253 | None:intergenic | 35.0% |
| !!! | AACTTGACTTGTTTTGTTGG+TGG | - | chr7.2:24840289-24840308 | None:intergenic | 35.0% |
| AAAGTAGTAGCACTAGGTTG+TGG | - | chr7.2:24840418-24840437 | None:intergenic | 40.0% | |
| AAGAACAAAGGTTGTGTTGG+TGG | + | chr7.2:24840457-24840476 | MS.gene55049:CDS | 40.0% | |
| CTAGGTTGTGGTATATTCGT+TGG | - | chr7.2:24840406-24840425 | None:intergenic | 40.0% | |
| GCTACTGCAAATGCTATCAA+CGG | + | chr7.2:24840619-24840638 | MS.gene55049:CDS | 40.0% | |
| GGTAGGAATAGTAGAGTTGT+GGG | + | chr7.2:24840646-24840665 | MS.gene55049:CDS | 40.0% | |
| GTAGGAATAGTAGAGTTGTG+GGG | + | chr7.2:24840647-24840666 | MS.gene55049:CDS | 40.0% | |
| ! | GGTGTTTTGTCAAGTTCAAG+AGG | + | chr7.2:24840481-24840500 | MS.gene55049:CDS | 40.0% |
| !!! | ACTTGTTTTGTTGGTGGAGT+TGG | - | chr7.2:24840283-24840302 | None:intergenic | 40.0% |
| GCAAATGCTATCAACGGTGA+GGG | + | chr7.2:24840625-24840644 | MS.gene55049:CDS | 45.0% | |
| GGGTAGGAATAGTAGAGTTG+TGG | + | chr7.2:24840645-24840664 | MS.gene55049:CDS | 45.0% | |
| GGTGTCAAAGTAGTAGCACT+AGG | - | chr7.2:24840424-24840443 | None:intergenic | 45.0% | |
| TGCAAATGCTATCAACGGTG+AGG | + | chr7.2:24840624-24840643 | MS.gene55049:CDS | 45.0% | |
| ! | AACAAAGGTTGTGTTGGTGG+TGG | + | chr7.2:24840460-24840479 | MS.gene55049:CDS | 45.0% |
| ATGCTATCAACGGTGAGGGT+AGG | + | chr7.2:24840629-24840648 | MS.gene55049:CDS | 50.0% | |
| GCTGCTGCAGAGAAGAGAAA+GGG | + | chr7.2:24840523-24840542 | MS.gene55049:CDS | 50.0% | |
| TGCTGCTGCAGAGAAGAGAA+AGG | + | chr7.2:24840522-24840541 | MS.gene55049:CDS | 50.0% | |
| ! | GCAGAGAAGAGAAAGGGTGT+TGG | + | chr7.2:24840529-24840548 | MS.gene55049:CDS | 50.0% |
| ! | GTGGTGGAATTCTGATGGCA+AGG | + | chr7.2:24840554-24840573 | MS.gene55049:CDS | 50.0% |
| !! | AAGAGAAAGGGTGTTGGTGG+TGG | + | chr7.2:24840535-24840554 | MS.gene55049:CDS | 50.0% |
| !! | GAGAAGAGAAAGGGTGTTGG+TGG | + | chr7.2:24840532-24840551 | MS.gene55049:CDS | 50.0% |
| !! | TGGTGGTGGTGGAATTCTGA+TGG | + | chr7.2:24840549-24840568 | MS.gene55049:CDS | 50.0% |
| !! | AGAAAGGGTGTTGGTGGTGG+TGG | + | chr7.2:24840538-24840557 | MS.gene55049:CDS | 55.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr7.2 | gene | 24840142 | 24840708 | 24840142 | ID=MS.gene55049 |
| chr7.2 | mRNA | 24840142 | 24840708 | 24840142 | ID=MS.gene55049.t1;Parent=MS.gene55049 |
| chr7.2 | exon | 24840142 | 24840708 | 24840142 | ID=MS.gene55049.t1.exon1;Parent=MS.gene55049.t1 |
| chr7.2 | CDS | 24840142 | 24840708 | 24840142 | ID=cds.MS.gene55049.t1;Parent=MS.gene55049.t1 |
| Gene Sequence |
| Protein sequence |
