AlfalfaGEDB | MS.gene55261

M. sativa cultivar XinJiangDaYe / MS.gene55261

KEGG: K15032
GO: GO:0003674 GO:0003676 GO:0003677 GO:0003690 GO:0005488 GO:0006355 GO:0008150 GO:0009889 GO:0010468 GO:0010556 GO:0019219 GO:0019222 GO:0031323 GO:0031326 GO:0050789 GO:0050794 GO:0051171 GO:0051252 GO:0060255 GO:0065007 GO:0080090 GO:0097159 GO:1901363 GO:1903506 GO:2000112 GO:2001141
PFAM: PF02536.17

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55261.t1	XP_004509519.1	91.1	202	18	0	1	202	110	311	7.00E-82	313.5

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55261.t1	Q84X53	45.3	203	110	1	1	202	82	284	2.4e-38	160.2

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55261.t1	A0A1S2YT68	91.1	202	18	0	1	202	110	311	5.0e-82	313.5

TFs/TRs:

Gene ID	Type	Classification
MS.gene55261.t1	TR	mTERF

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene052190	MS.gene55261	0.809019	2.33E-50	-1.69E-46
MS.gene052582	MS.gene55261	0.802798	4.73E-49	-1.69E-46
MS.gene052584	MS.gene55261	0.807502	4.90E-50	-1.69E-46
MS.gene053226	MS.gene55261	0.825485	4.57E-54	-1.69E-46
MS.gene054291	MS.gene55261	-0.811498	6.79E-51	-1.69E-46
MS.gene055166	MS.gene55261	0.821666	3.58E-53	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55261.t1	MTR_5g041630	93.000	200	13	1	1	200	106	304	9.01e-129	365
MS.gene55261.t1	MTR_2g049780	49.189	185	94	0	3	187	120	304	1.84e-56	181
MS.gene55261.t1	MTR_2g067230	40.314	191	105	3	3	191	120	303	3.04e-42	145
MS.gene55261.t1	MTR_7g081270	37.113	194	111	4	8	191	91	283	6.07e-30	112
MS.gene55261.t1	MTR_2g034600	33.862	189	119	5	1	188	300	483	1.02e-23	97.8
MS.gene55261.t1	MTR_2g034600	33.862	189	119	5	1	188	361	544	1.64e-23	97.4
MS.gene55261.t1	MTR_2g034600	33.862	189	119	5	1	188	365	548	1.82e-23	97.4
MS.gene55261.t1	MTR_5g094610	30.636	173	117	1	1	170	279	451	1.82e-20	89.0
MS.gene55261.t1	MTR_6g003960	28.125	192	134	3	3	192	403	592	1.02e-17	80.9
MS.gene55261.t1	MTR_4g084630	27.513	189	135	1	1	187	130	318	7.66e-16	74.7
MS.gene55261.t1	MTR_4g070060	26.904	197	139	3	1	194	398	592	1.31e-14	72.0
MS.gene55261.t1	MTR_1g115415	37.838	111	64	2	83	191	347	454	2.76e-14	70.9
MS.gene55261.t1	MTR_5g068860	32.787	122	77	2	83	200	379	499	1.26e-12	66.2

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55261.t1	AT2G03050	62.069	203	76	1	1	202	81	283	2.34e-83	249
MS.gene55261.t1	AT2G03050	62.069	203	76	1	1	202	82	284	2.55e-83	249
MS.gene55261.t1	AT2G34620	45.078	193	106	0	1	193	104	296	3.02e-54	175
MS.gene55261.t1	AT2G36000	40.000	190	109	2	3	191	126	311	2.40e-40	140
MS.gene55261.t1	AT2G36000	40.323	186	106	2	3	187	126	307	3.34e-40	140
MS.gene55261.t1	AT3G18870	36.458	192	116	3	3	191	84	272	5.08e-31	114
MS.gene55261.t1	AT1G78930	34.343	198	122	3	1	194	388	581	1.89e-27	108
MS.gene55261.t1	AT4G14605	31.217	189	124	5	1	188	285	468	1.90e-21	91.7
MS.gene55261.t1	AT4G14605	31.217	189	124	5	1	188	285	468	1.90e-21	91.7
MS.gene55261.t1	AT5G55580	31.429	175	113	3	1	170	166	338	1.36e-20	88.6
MS.gene55261.t1	AT5G55580	31.429	175	113	3	1	170	272	444	3.91e-20	87.8
MS.gene55261.t1	AT2G21710	30.102	196	132	3	1	193	405	598	1.64e-18	83.2
MS.gene55261.t1	AT4G38160	28.025	157	110	1	38	191	168	324	2.44e-14	70.5
MS.gene55261.t1	AT4G38160	28.025	157	110	1	38	191	168	324	2.44e-14	70.5
MS.gene55261.t1	AT4G38160	28.025	157	110	1	38	191	168	324	2.58e-14	70.9
MS.gene55261.t1	AT4G38160	28.025	157	110	1	38	191	168	324	3.05e-14	70.5
MS.gene55261.t1	AT2G44020	36.937	111	65	2	83	191	352	459	8.51e-14	69.7
MS.gene55261.t1	AT5G55580	29.114	158	100	5	24	172	223	377	4.81e-11	61.2

Find 40 sgRNAs with CRISPR-Local

Find 65 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GTTGCTTGATTTGCGGTTAA+AGG	0.233881	5.2:-31208617	MS.gene55261:CDS
ACGTGTGGAGGTTTCGAATA+TGG	0.257392	5.2:-31208875	MS.gene55261:CDS
GTGCCAGACGACATTGCTTT+TGG	0.336067	5.2:-31208959	MS.gene55261:CDS
TTGAGGGAATTAGGGTTTGT+TGG	0.348767	5.2:-31208997	MS.gene55261:CDS
TGAGGGAATTAGGGTTTGTT+GGG	0.358434	5.2:-31208996	MS.gene55261:CDS
AATATGGTTGTGAGGTCACC+TGG	0.384205	5.2:-31208859	MS.gene55261:CDS
GGTTCACACGTTAGAAGTTC+AGG	0.397383	5.2:+31209162	None:intergenic
TCCTTTGCAAGAGATGTTGC+AGG	0.404002	5.2:-31208665	MS.gene55261:CDS
AAAACTCAACTTTAGGCAAA+AGG	0.404268	5.2:+31208917	None:intergenic
CACGCGATGTTGGTTCGGCT+TGG	0.408581	5.2:-31208697	MS.gene55261:CDS
CTTGCGGTGTCCTAGATTGT+TGG	0.417943	5.2:-31209061	MS.gene55261:CDS
TCGTCTGGCACGTGAGAGAA+TGG	0.446727	5.2:+31208971	None:intergenic
GGAGGCACGCGATGTTGGTT+CGG	0.447113	5.2:-31208702	MS.gene55261:CDS
ATTCTAGGTTGCTTGATTTG+CGG	0.448500	5.2:-31208624	MS.gene55261:CDS
ACACTACTAACCAACAATCT+AGG	0.454380	5.2:+31209051	None:intergenic
AAGGAACTTGAGGGAAGATA+AGG	0.475839	5.2:-31208598	None:intergenic
CGTCTGGCACGTGAGAGAAT+GGG	0.495297	5.2:+31208972	None:intergenic
AGGAACTTGAGGGAAGATAA+GGG	0.503033	5.2:-31208597	None:intergenic
AAACCAAAAGCAATGTCGTC+TGG	0.503937	5.2:+31208956	None:intergenic
GGGTTTGGGGTTCACACGTG+TGG	0.504748	5.2:-31208890	MS.gene55261:CDS
AAACTCAACTTTAGGCAAAA+GGG	0.506451	5.2:+31208918	None:intergenic
ATGTTGCAGGTTAGTGATGG+TGG	0.519665	5.2:-31208652	MS.gene55261:CDS
ATTGAGAAGAAAATCAAGAA+GGG	0.544003	5.2:+31209122	None:intergenic
TAAGCGGAGGCACGCGATGT+TGG	0.548999	5.2:-31208707	MS.gene55261:CDS
TTTGGGGTTCACACGTGTGG+AGG	0.549501	5.2:-31208887	MS.gene55261:CDS
CATTGAGAAGAAAATCAAGA+AGG	0.567057	5.2:+31209121	None:intergenic
TTGCGGTTAAAGGAACTTGA+GGG	0.570571	5.2:-31208607	MS.gene55261:CDS
GTCTGGCACGTGAGAGAATG+GGG	0.572333	5.2:+31208973	None:intergenic
ACCTGCAACATCTCTTGCAA+AGG	0.590277	5.2:+31208664	None:intergenic
TTTGCGGTTAAAGGAACTTG+AGG	0.597296	5.2:-31208608	MS.gene55261:CDS
GCTTGGAAGGGAGGATTAAG+CGG	0.607101	5.2:-31208723	MS.gene55261:CDS
TTGAGAAGAAAATCAAGAAG+GGG	0.620983	5.2:+31209123	None:intergenic
GTGATGTTGCTGAGTTGAAG+AGG	0.653205	5.2:-31208768	MS.gene55261:CDS
GAGATGTTGCAGGTTAGTGA+TGG	0.656683	5.2:-31208655	MS.gene55261:CDS
AGGTTTCGAATATGGTTGTG+AGG	0.659704	5.2:-31208867	MS.gene55261:CDS
AAGCTAAAAGAGAAATACTG+AGG	0.664066	5.2:+31208742	None:intergenic
TGAGAAGAAAATCAAGAAGG+GGG	0.672373	5.2:+31209124	None:intergenic
TGGAAGGGAGGATTAAGCGG+AGG	0.683228	5.2:-31208720	MS.gene55261:CDS
ACACTAAAAGTCAATAACCC+AGG	0.692067	5.2:+31208841	None:intergenic
ATATGGTTGTGAGGTCACCT+GGG	0.703831	5.2:-31208858	MS.gene55261:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!	ATGTTCAAAAATCAATCTTG+CGG	-	chr5.2:31208709-31208728	MS.gene55261:CDS	25.0%
!	ATTGAGAAGAAAATCAAGAA+GGG	+	chr5.2:31208667-31208686	None:intergenic	25.0%
!	TTCACGAAAAACTCAAATTT+AGG	+	chr5.2:31208987-31209006	None:intergenic	25.0%
!!	TTTTAGTGTGGATAAGAATT+TGG	-	chr5.2:31208959-31208978	MS.gene55261:CDS	25.0%
!!!	AGATTGATTTTTGAACATCA+TGG	+	chr5.2:31208707-31208726	None:intergenic	25.0%
!!!	TAAATTTGAGTTTTTCGTGA+AGG	-	chr5.2:31208986-31209005	MS.gene55261:CDS	25.0%
	AAAACTCAACTTTAGGCAAA+AGG	+	chr5.2:31208872-31208891	None:intergenic	30.0%
	AAACTCAACTTTAGGCAAAA+GGG	+	chr5.2:31208871-31208890	None:intergenic	30.0%
	AAGCTAAAAGAGAAATACTG+AGG	+	chr5.2:31209047-31209066	None:intergenic	30.0%
	CATTGAGAAGAAAATCAAGA+AGG	+	chr5.2:31208668-31208687	None:intergenic	30.0%
	TTGAGAAGAAAATCAAGAAG+GGG	+	chr5.2:31208666-31208685	None:intergenic	30.0%
!	CCTAAAGTTGAGTTTTTGAT+GGG	-	chr5.2:31208876-31208895	MS.gene55261:CDS	30.0%
!	GATTTTTGAACATCATGGTA+AGG	+	chr5.2:31208702-31208721	None:intergenic	30.0%
!	TCTTGATTTTCTTCTCAATG+AGG	-	chr5.2:31208668-31208687	MS.gene55261:CDS	30.0%
!!	TCAGTATTTCTCTTTTAGCT+TGG	-	chr5.2:31209046-31209065	MS.gene55261:CDS	30.0%
!!!	ATTTCTCTTTTAGCTTGGAA+GGG	-	chr5.2:31209051-31209070	MS.gene55261:CDS	30.0%
!!!	TATTTCTCTTTTAGCTTGGA+AGG	-	chr5.2:31209050-31209069	MS.gene55261:CDS	30.0%
!!!	TGTGTTTTTTGAGGGAATTA+GGG	-	chr5.2:31208781-31208800	MS.gene55261:CDS	30.0%
	ACACTAAAAGTCAATAACCC+AGG	+	chr5.2:31208948-31208967	None:intergenic	35.0%
	ACACTACTAACCAACAATCT+AGG	+	chr5.2:31208738-31208757	None:intergenic	35.0%
	AGGGGGTAAATATCATTATG+CGG	+	chr5.2:31208648-31208667	None:intergenic	35.0%
	CCCATCAAAAACTCAACTTT+AGG	+	chr5.2:31208879-31208898	None:intergenic	35.0%
	TGAGAAGAAAATCAAGAAGG+GGG	+	chr5.2:31208665-31208684	None:intergenic	35.0%
!	ATTCTAGGTTGCTTGATTTG+CGG	-	chr5.2:31209162-31209181	MS.gene55261:CDS	35.0%
!	GCCTAAAGTTGAGTTTTTGA+TGG	-	chr5.2:31208875-31208894	MS.gene55261:CDS	35.0%
!	TTTTTCGTGAAGGAGATGAA+TGG	-	chr5.2:31208996-31209015	MS.gene55261:CDS	35.0%
!!!	AGTTGAGTTTTTGATGGGTT+TGG	-	chr5.2:31208881-31208900	MS.gene55261:CDS	35.0%
!!!	CTGTGTTTTTTGAGGGAATT+AGG	-	chr5.2:31208780-31208799	MS.gene55261:CDS	35.0%
!!!	GTTGAGTTTTTGATGGGTTT+GGG	-	chr5.2:31208882-31208901	MS.gene55261:CDS	35.0%
!!!	TGGGTTATTGACTTTTAGTG+TGG	-	chr5.2:31208947-31208966	MS.gene55261:CDS	35.0%
!!!	TTGAGTTTTTGATGGGTTTG+GGG	-	chr5.2:31208883-31208902	MS.gene55261:CDS	35.0%
	AAACCAAAAGCAATGTCGTC+TGG	+	chr5.2:31208833-31208852	None:intergenic	40.0%
	AGGTTTCGAATATGGTTGTG+AGG	-	chr5.2:31208919-31208938	MS.gene55261:CDS	40.0%
	TTGAGGGAATTAGGGTTTGT+TGG	-	chr5.2:31208789-31208808	MS.gene55261:CDS	40.0%
	TTGCGGTTAAAGGAACTTGA+GGG	-	chr5.2:31209179-31209198	MS.gene55261:CDS	40.0%
	TTTGCGGTTAAAGGAACTTG+AGG	-	chr5.2:31209178-31209197	MS.gene55261:CDS	40.0%
!	TGAGGGAATTAGGGTTTGTT+GGG	-	chr5.2:31208790-31208809	MS.gene55261:CDS	40.0%
!!	GTTGCTTGATTTGCGGTTAA+AGG	-	chr5.2:31209169-31209188	MS.gene55261:CDS	40.0%
!!!	ACCTGCTCTGTGTTTTTTGA+GGG	-	chr5.2:31208773-31208792	MS.gene55261:CDS	40.0%
!!!	GTGATGGTGGTTTTGATTCT+AGG	-	chr5.2:31209147-31209166	MS.gene55261:CDS	40.0%
	AATATGGTTGTGAGGTCACC+TGG	-	chr5.2:31208927-31208946	MS.gene55261:CDS	45.0%
	ACCTGCAACATCTCTTGCAA+AGG	+	chr5.2:31209125-31209144	None:intergenic	45.0%
	ACGTGTGGAGGTTTCGAATA+TGG	-	chr5.2:31208911-31208930	MS.gene55261:CDS	45.0%
	ATATGGTTGTGAGGTCACCT+GGG	-	chr5.2:31208928-31208947	MS.gene55261:CDS	45.0%
	ATGTTGCAGGTTAGTGATGG+TGG	-	chr5.2:31209134-31209153	MS.gene55261:CDS	45.0%
	GAGATGTTGCAGGTTAGTGA+TGG	-	chr5.2:31209131-31209150	MS.gene55261:CDS	45.0%
	GGTTCACACGTTAGAAGTTC+AGG	+	chr5.2:31208627-31208646	None:intergenic	45.0%
	TCCCTCAAAAAACACAGAGC+AGG	+	chr5.2:31208777-31208796	None:intergenic	45.0%
	TCCTTTGCAAGAGATGTTGC+AGG	-	chr5.2:31209121-31209140	MS.gene55261:CDS	45.0%
!	GTGATGTTGCTGAGTTGAAG+AGG	-	chr5.2:31209018-31209037	MS.gene55261:CDS	45.0%
!!!	GACCTGCTCTGTGTTTTTTG+AGG	-	chr5.2:31208772-31208791	MS.gene55261:CDS	45.0%
!!!	GCTTTTGGTTTCGAGTGTTG+AGG	-	chr5.2:31208842-31208861	MS.gene55261:CDS	45.0%
!!!	TCTCTTTTAGCTTGGAAGGG+AGG	-	chr5.2:31209054-31209073	MS.gene55261:CDS	45.0%
	CTTGCGGTGTCCTAGATTGT+TGG	-	chr5.2:31208725-31208744	MS.gene55261:CDS	50.0%
	GCTTGGAAGGGAGGATTAAG+CGG	-	chr5.2:31209063-31209082	MS.gene55261:CDS	50.0%
!	GTGCCAGACGACATTGCTTT+TGG	-	chr5.2:31208827-31208846	MS.gene55261:CDS	50.0%
	CGTCTGGCACGTGAGAGAAT+GGG	+	chr5.2:31208817-31208836	None:intergenic	55.0%
	GTCTGGCACGTGAGAGAATG+GGG	+	chr5.2:31208816-31208835	None:intergenic	55.0%
	TCGTCTGGCACGTGAGAGAA+TGG	+	chr5.2:31208818-31208837	None:intergenic	55.0%
	TGGAAGGGAGGATTAAGCGG+AGG	-	chr5.2:31209066-31209085	MS.gene55261:CDS	55.0%
!	TTTGGGGTTCACACGTGTGG+AGG	-	chr5.2:31208899-31208918	MS.gene55261:CDS	55.0%
!	GGAGGCACGCGATGTTGGTT+CGG	-	chr5.2:31209084-31209103	MS.gene55261:CDS	60.0%
!	GGGTTTGGGGTTCACACGTG+TGG	-	chr5.2:31208896-31208915	MS.gene55261:CDS	60.0%
!	TAAGCGGAGGCACGCGATGT+TGG	-	chr5.2:31209079-31209098	MS.gene55261:CDS	60.0%
!!	CACGCGATGTTGGTTCGGCT+TGG	-	chr5.2:31209089-31209108	MS.gene55261:CDS	60.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	31208600	31209208	31208600	ID=MS.gene55261
chr5.2	mRNA	31208600	31209208	31208600	ID=MS.gene55261.t1;Parent=MS.gene55261
chr5.2	exon	31208600	31209208	31208600	ID=MS.gene55261.t1.exon1;Parent=MS.gene55261.t1
chr5.2	CDS	31208600	31209208	31208600	ID=cds.MS.gene55261.t1;Parent=MS.gene55261.t1

Gene Sequence

>MS.gene55261

ATGGGTCGCATTCTTGATATGCTTCCTGAACTTCTAACGTGTGAACCGCATAATGATATTTACCCCCTTCTTGATTTTCTTCTCAATGAGGTTGAAATTCCTTACCATGATGTTCAAAAATCAATCTTGCGGTGTCCTAGATTGTTGGTTAGTAGTGTAGAGAATCGATTGAGACCTGCTCTGTGTTTTTTGAGGGAATTAGGGTTTGTTGGGCCCCATTCTCTCACGTGCCAGACGACATTGCTTTTGGTTTCGAGTGTTGAGGATACCCTTTTGCCTAAAGTTGAGTTTTTGATGGGTTTGGGGTTCACACGTGTGGAGGTTTCGAATATGGTTGTGAGGTCACCTGGGTTATTGACTTTTAGTGTGGATAAGAATTTGGCTCCTAAATTTGAGTTTTTCGTGAAGGAGATGAATGGTGATGTTGCTGAGTTGAAGAGGTATCCTCAGTATTTCTCTTTTAGCTTGGAAGGGAGGATTAAGCGGAGGCACGCGATGTTGGTTCGGCTTGGATTGTCGCTTCCTTTGCAAGAGATGTTGCAGGTTAGTGATGGTGGTTTTGATTCTAGGTTGCTTGATTTGCGGTTAAAGGAACTTGAGGGAAGATAA

Protein sequence

>MS.gene55261.t1

MGRILDMLPELLTCEPHNDIYPLLDFLLNEVEIPYHDVQKSILRCPRLLVSSVENRLRPALCFLRELGFVGPHSLTCQTTLLLVSSVEDTLLPKVEFLMGLGFTRVEVSNMVVRSPGLLTFSVDKNLAPKFEFFVKEMNGDVAELKRYPQYFSFSLEGRIKRRHAMLVRLGLSLPLQEMLQVSDGGFDSRLLDLRLKELEGR