AlfalfaGEDB | MS.gene55285

M. sativa cultivar XinJiangDaYe / MS.gene55285

KEGG: -
GO: -
PFAM: -

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55285.t1	XP_013464597.1	77.9	86	18	1	1	85	1	86	1.80E-23	118.2

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55285.t1	A0A072V9Y5	77.9	86	18	1	1	85	1	86	1.3e-23	118.2

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR
MS.gene049254	MS.gene55285	0.807161	5.79E-50	-1.69E-46
MS.gene049821	MS.gene55285	0.845762	3.32E-59	-1.69E-46
MS.gene052582	MS.gene55285	0.808084	3.68E-50	-1.69E-46
MS.gene052584	MS.gene55285	0.819415	1.18E-52	-1.69E-46
MS.gene05415	MS.gene55285	0.833143	6.32E-56	-1.69E-46
MS.gene05423	MS.gene55285	0.819285	1.26E-52	-1.69E-46
MS.gene056174	MS.gene55285	0.804382	2.22E-49	-1.69E-46
MS.gene058979	MS.gene55285	0.804484	2.11E-49	-1.69E-46
MS.gene060365	MS.gene55285	0.80744	5.05E-50	-1.69E-46
MS.gene060634	MS.gene55285	-0.817543	3.12E-52	-1.69E-46

PPI:

Gene1	Gene2	Type

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene55285.t1	MTR_2g078090	87.209	86	10	1	1	85	1	86	7.34e-32	114
MS.gene55285.t1	MTR_1g009840	68.605	86	18	3	1	85	1	78	5.67e-26	97.8
MS.gene55285.t1	MTR_2g078100	59.302	86	23	3	1	85	1	75	3.41e-16	67.8

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
GGTGGTTAGGGTTAGGGTTT+TGG	0.131447	5.2:+31713398	None:intergenic
GAAGGAGAGAGCGGAGGAAG+AGG	0.281712	5.2:+31713338	None:intergenic
GAGGAAGGTGGTTAGGGTTA+GGG	0.291280	5.2:+31713392	None:intergenic
AGAGGAAGGTGGTTAGGGTT+AGG	0.294206	5.2:+31713391	None:intergenic
AGCGAGAGAGGAAGGTGGTT+AGG	0.323331	5.2:+31713385	None:intergenic
AGTTGACGATTGGACAAGTT+AGG	0.340015	5.2:+31713204	None:intergenic
GCGAGAGAGGAAGGTGGTTA+GGG	0.384328	5.2:+31713386	None:intergenic
GAGAGCGGAGGAAGAGGGAG+TGG	0.391057	5.2:+31713344	None:intergenic
AGAGGGGACGGAGACGGCGG+AGG	0.405451	5.2:+31713316	None:intergenic
AGAGGGAGTGGTGGTAGTGG+TGG	0.417356	5.2:+31713356	None:intergenic
CGATTGGACAAGTTAGGGTT+CGG	0.427838	5.2:+31713210	None:intergenic
AAGGAGAGAGCGGAGGAAGA+GGG	0.435215	5.2:+31713339	None:intergenic
TGGTGGAGATGGAGCGAGAG+AGG	0.435756	5.2:+31713373	None:intergenic
GGAGATGGAGCGAGAGAGGA+AGG	0.440256	5.2:+31713377	None:intergenic
CGGCAGAGAGGGGACGGAGA+CGG	0.446029	5.2:+31713310	None:intergenic
AGTGGTGGTAGTGGTGGAGA+TGG	0.447238	5.2:+31713362	None:intergenic
TCGTTGAGGGAAAAGGTAGT+AGG	0.453529	5.2:+31713234	None:intergenic
GGAAGAGGGAGTGGTGGTAG+TGG	0.453659	5.2:+31713353	None:intergenic
CATTTCCTTTAGTTGACGAT+TGG	0.462046	5.2:+31713194	None:intergenic
GATGATGGCGGCGGCAGAGA+GGG	0.463552	5.2:+31713299	None:intergenic
GGGACGGAGACGGCGGAGGA+AGG	0.465477	5.2:+31713320	None:intergenic
GTGTGGAGAAAGCGCGGAAG+GGG	0.475092	5.2:+31713259	None:intergenic
AGCGGAGGAAGAGGGAGTGG+TGG	0.477978	5.2:+31713347	None:intergenic
GAGGATGTGACGGGAGATGA+TGG	0.480132	5.2:+31713284	None:intergenic
GGAGAACGCGAGAGAAGATC+TGG	0.480186	5.2:+31713421	None:intergenic
AGAAAGCGCGGAAGGGGAAG+AGG	0.492275	5.2:+31713265	None:intergenic
CTTGTCCAATCGTCAACTAA+AGG	0.494332	5.2:-31713199	MS.gene55285:CDS
GTTAGGGTTCGGATCGTTGA+GGG	0.494823	5.2:+31713221	None:intergenic
GTTCGGATCGTTGAGGGAAA+AGG	0.504026	5.2:+31713227	None:intergenic
TGGCGGCGGCAGAGAGGGGA+CGG	0.511676	5.2:+31713304	None:intergenic
GCGTGTGGAGAAAGCGCGGA+AGG	0.513615	5.2:+31713257	None:intergenic
GTTGACGATTGGACAAGTTA+GGG	0.515268	5.2:+31713205	None:intergenic
ACGGCGGAGGAAGGAGAGAG+CGG	0.522664	5.2:+31713329	None:intergenic
ATGATGGCGGCGGCAGAGAG+GGG	0.529506	5.2:+31713300	None:intergenic
CAGAGAGGGGACGGAGACGG+CGG	0.530785	5.2:+31713313	None:intergenic
GAAGGGGAAGAGGATGTGAC+GGG	0.531441	5.2:+31713275	None:intergenic
GATGGAGCGAGAGAGGAAGG+TGG	0.551999	5.2:+31713380	None:intergenic
GGAAAAGGTAGTAGGGCGTG+TGG	0.560396	5.2:+31713242	None:intergenic
CGTTGAGGGAAAAGGTAGTA+GGG	0.561027	5.2:+31713235	None:intergenic
CGTGTGGAGAAAGCGCGGAA+GGG	0.563716	5.2:+31713258	None:intergenic
GGAAGGGGAAGAGGATGTGA+CGG	0.581551	5.2:+31713274	None:intergenic
GCGGAGGAAGGAGAGAGCGG+AGG	0.602658	5.2:+31713332	None:intergenic
AGATGATGGCGGCGGCAGAG+AGG	0.603366	5.2:+31713298	None:intergenic
AGTTAGGGTTCGGATCGTTG+AGG	0.617571	5.2:+31713220	None:intergenic
GTGACGGGAGATGATGGCGG+CGG	0.617812	5.2:+31713290	None:intergenic
TAGGGCGTGTGGAGAAAGCG+CGG	0.685123	5.2:+31713253	None:intergenic
GATGTGACGGGAGATGATGG+CGG	0.719758	5.2:+31713287	None:intergenic

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
	CATTTCCTTTAGTTGACGAT+TGG	+	chr5.2:31713431-31713450	None:intergenic	35.0%
	AGTTGACGATTGGACAAGTT+AGG	+	chr5.2:31713421-31713440	None:intergenic	40.0%
	CTTGTCCAATCGTCAACTAA+AGG	-	chr5.2:31713423-31713442	MS.gene55285:CDS	40.0%
	GTTGACGATTGGACAAGTTA+GGG	+	chr5.2:31713420-31713439	None:intergenic	40.0%
	CGATTGGACAAGTTAGGGTT+CGG	+	chr5.2:31713415-31713434	None:intergenic	45.0%
	CGTTGAGGGAAAAGGTAGTA+GGG	+	chr5.2:31713390-31713409	None:intergenic	45.0%
	TCGTTGAGGGAAAAGGTAGT+AGG	+	chr5.2:31713391-31713410	None:intergenic	45.0%
!	GTGGTTAGGGTTAGGGTTTT+GGG	+	chr5.2:31713226-31713245	None:intergenic	45.0%
!	TGGTTAGGGTTAGGGTTTTG+GGG	+	chr5.2:31713225-31713244	None:intergenic	45.0%
	AGAGGAAGGTGGTTAGGGTT+AGG	+	chr5.2:31713234-31713253	None:intergenic	50.0%
	AGTTAGGGTTCGGATCGTTG+AGG	+	chr5.2:31713405-31713424	None:intergenic	50.0%
	GAGGAAGGTGGTTAGGGTTA+GGG	+	chr5.2:31713233-31713252	None:intergenic	50.0%
	GTTAGGGTTCGGATCGTTGA+GGG	+	chr5.2:31713404-31713423	None:intergenic	50.0%
	GTTCGGATCGTTGAGGGAAA+AGG	+	chr5.2:31713398-31713417	None:intergenic	50.0%
!	GGTGGTTAGGGTTAGGGTTT+TGG	+	chr5.2:31713227-31713246	None:intergenic	50.0%
	AAGGAGAGAGCGGAGGAAGA+GGG	+	chr5.2:31713286-31713305	None:intergenic	55.0%
	AGCGAGAGAGGAAGGTGGTT+AGG	+	chr5.2:31713240-31713259	None:intergenic	55.0%
	GAAGGGGAAGAGGATGTGAC+GGG	+	chr5.2:31713350-31713369	None:intergenic	55.0%
	GCGAGAGAGGAAGGTGGTTA+GGG	+	chr5.2:31713239-31713258	None:intergenic	55.0%
	GGAAAAGGTAGTAGGGCGTG+TGG	+	chr5.2:31713383-31713402	None:intergenic	55.0%
	GGAAGGGGAAGAGGATGTGA+CGG	+	chr5.2:31713351-31713370	None:intergenic	55.0%
	GGAGAACGCGAGAGAAGATC+TGG	+	chr5.2:31713204-31713223	None:intergenic	55.0%
!	GAGGATGTGACGGGAGATGA+TGG	+	chr5.2:31713341-31713360	None:intergenic	55.0%
!	GATGTGACGGGAGATGATGG+CGG	+	chr5.2:31713338-31713357	None:intergenic	55.0%
!!	AGTGGTGGTAGTGGTGGAGA+TGG	+	chr5.2:31713263-31713282	None:intergenic	55.0%
	GAAGGAGAGAGCGGAGGAAG+AGG	+	chr5.2:31713287-31713306	None:intergenic	60.0%
	GATGGAGCGAGAGAGGAAGG+TGG	+	chr5.2:31713245-31713264	None:intergenic	60.0%
	GGAAGAGGGAGTGGTGGTAG+TGG	+	chr5.2:31713272-31713291	None:intergenic	60.0%
	GGAGATGGAGCGAGAGAGGA+AGG	+	chr5.2:31713248-31713267	None:intergenic	60.0%
	TAGGGCGTGTGGAGAAAGCG+CGG	+	chr5.2:31713372-31713391	None:intergenic	60.0%
	TGGTGGAGATGGAGCGAGAG+AGG	+	chr5.2:31713252-31713271	None:intergenic	60.0%
!	AGAAAGCGCGGAAGGGGAAG+AGG	+	chr5.2:31713360-31713379	None:intergenic	60.0%
!	CGTGTGGAGAAAGCGCGGAA+GGG	+	chr5.2:31713367-31713386	None:intergenic	60.0%
!	GTGTGGAGAAAGCGCGGAAG+GGG	+	chr5.2:31713366-31713385	None:intergenic	60.0%
!!	AGAGGGAGTGGTGGTAGTGG+TGG	+	chr5.2:31713269-31713288	None:intergenic	60.0%
	ACGGCGGAGGAAGGAGAGAG+CGG	+	chr5.2:31713296-31713315	None:intergenic	65.0%
	AGCGGAGGAAGAGGGAGTGG+TGG	+	chr5.2:31713278-31713297	None:intergenic	65.0%
	GAGAGCGGAGGAAGAGGGAG+TGG	+	chr5.2:31713281-31713300	None:intergenic	65.0%
!	GCGTGTGGAGAAAGCGCGGA+AGG	+	chr5.2:31713368-31713387	None:intergenic	65.0%
!!	AGATGATGGCGGCGGCAGAG+AGG	+	chr5.2:31713327-31713346	None:intergenic	65.0%
!!	ATGATGGCGGCGGCAGAGAG+GGG	+	chr5.2:31713325-31713344	None:intergenic	65.0%
!!	GATGATGGCGGCGGCAGAGA+GGG	+	chr5.2:31713326-31713345	None:intergenic	65.0%
!!	GTGACGGGAGATGATGGCGG+CGG	+	chr5.2:31713335-31713354	None:intergenic	65.0%
	CAGAGAGGGGACGGAGACGG+CGG	+	chr5.2:31713312-31713331	None:intergenic	70.0%
	CGGCAGAGAGGGGACGGAGA+CGG	+	chr5.2:31713315-31713334	None:intergenic	70.0%
	GCGGAGGAAGGAGAGAGCGG+AGG	+	chr5.2:31713293-31713312	None:intergenic	70.0%
	AGAGGGGACGGAGACGGCGG+AGG	+	chr5.2:31713309-31713328	None:intergenic	75.0%
	GGGACGGAGACGGCGGAGGA+AGG	+	chr5.2:31713305-31713324	None:intergenic	75.0%
	TGGCGGCGGCAGAGAGGGGA+CGG	+	chr5.2:31713321-31713340	None:intergenic	75.0%

Chromosome	Type	Strat	End	Strand	Name
chr5.2	gene	31713193	31713451	31713193	ID=MS.gene55285
chr5.2	mRNA	31713193	31713451	31713193	ID=MS.gene55285.t1;Parent=MS.gene55285
chr5.2	exon	31713193	31713451	31713193	ID=MS.gene55285.t1.exon1;Parent=MS.gene55285.t1
chr5.2	CDS	31713193	31713451	31713193	ID=cds.MS.gene55285.t1;Parent=MS.gene55285.t1

Gene Sequence	>MS.gene55285 ATGGCGAACCAGATCTTCTCTCGCGTTCTCCCCAAAACCCTAACCCTAACCACCTTCCTCTCTCGCTCCATCTCCACCACTACCACCACTCCCTCTTCCTCCGCTCTCTCCTTCCTCCGCCGTCTCCGTCCCCTCTCTGCCGCCGCCATCATCTCCCGTCACATCCTCTTCCCCTTCCGCGCTTTCTCCACACGCCCTACTACCTTTTCCCTCAACGATCCGAACCCTAACTTGTCCAATCGTCAACTAAAGGAAATGA
Protein sequence	>MS.gene55285.t1 MANQIFSRVLPKTLTLTTFLSRSISTTTTTPSSSALSFLRRLRPLSAAAIISRHILFPFRAFSTRPTTFSLNDPNPNLSNRQLKEM