Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55727.t1 | XP_013447087.1 | 98.4 | 192 | 1 | 1 | 1 | 192 | 431 | 620 | 2.30E-82 | 315.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55727.t1 | Q38897 | 44.2 | 156 | 59 | 11 | 42 | 191 | 477 | 610 | 3.0e-14 | 80.1 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55727.t1 | A0A072TUA6 | 98.4 | 192 | 1 | 1 | 1 | 192 | 431 | 620 | 1.6e-82 | 315.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene05995 | MS.gene55727 | 0.843658 | 1.22E-58 | -1.69E-46 |
| MS.gene05996 | MS.gene55727 | 0.838357 | 3.02E-57 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55727.t1 | MTR_8g098815 | 98.958 | 192 | 0 | 1 | 1 | 192 | 431 | 620 | 5.96e-135 | 391 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene55727.t1 | AT5G41410 | 38.889 | 198 | 76 | 12 | 1 | 192 | 453 | 611 | 5.96e-22 | 92.8 |
Find 46 sgRNAs with CRISPR-Local
Find 48 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ACCGGTTCAGTACTCTCTTT+TGG | 0.253768 | 4.3:-5542762 | MS.gene55727:CDS |
| AATGGTGGAAGAAATGTATT+TGG | 0.286857 | 4.3:-5543248 | MS.gene55727:CDS |
| ACTGAACCGGTTCTTCAATT+TGG | 0.298745 | 4.3:+5542774 | None:intergenic |
| CAAACGCATCCGCAGCTAAT+TGG | 0.298890 | 4.3:+5543002 | None:intergenic |
| CATGAGGTTGAGTTTGAGAT+TGG | 0.355537 | 4.3:+5543134 | None:intergenic |
| CAATTAGCTGCGGATGCGTT+TGG | 0.368691 | 4.3:-5543001 | MS.gene55727:CDS |
| GGTGGAGTGTCATTGACGTT+AGG | 0.399182 | 4.3:-5542875 | MS.gene55727:CDS |
| TTACAACAACATGGTGGAAA+TGG | 0.409545 | 4.3:-5542851 | MS.gene55727:CDS |
| CTCCACCACTGCTGCCACCT+TGG | 0.420187 | 4.3:+5542891 | None:intergenic |
| TGGTGGAAATGGAGTGAGTT+TGG | 0.455316 | 4.3:-5542840 | MS.gene55727:CDS |
| AAATACATTTCTTCCACCAT+TGG | 0.455432 | 4.3:+5543250 | None:intergenic |
| ATGATTGTTGTTGATGATAG+AGG | 0.462600 | 4.3:+5543066 | None:intergenic |
| CACCACTGCTGCCACCTTGG+TGG | 0.462654 | 4.3:+5542894 | None:intergenic |
| AAGGGACCAAATTGAAGAAC+CGG | 0.465720 | 4.3:-5542780 | MS.gene55727:CDS |
| GTGGAGTGTCATTGACGTTA+GGG | 0.474230 | 4.3:-5542874 | MS.gene55727:CDS |
| GAACAAATATTATGCCATAC+AGG | 0.476848 | 4.3:-5542730 | MS.gene55727:CDS |
| CACCAAGGTGGCAGCAGTGG+TGG | 0.491693 | 4.3:-5542893 | MS.gene55727:CDS |
| AGCTGCGGATGCGTTTGGAT+CGG | 0.498921 | 4.3:-5542996 | MS.gene55727:CDS |
| TCCAAAAGAGAGTACTGAAC+CGG | 0.513134 | 4.3:+5542761 | None:intergenic |
| TTCCACCAAGGTGGCAGCAG+TGG | 0.532318 | 4.3:-5542896 | MS.gene55727:CDS |
| GACACAATTGCTACATGACT+TGG | 0.534189 | 4.3:-5542696 | MS.gene55727:CDS |
| ACGACTCCTTCGAATCGATT+CGG | 0.534329 | 4.3:-5543098 | MS.gene55727:CDS |
| AACACGACAACCTCGGACGG+AGG | 0.540366 | 4.3:-5543202 | MS.gene55727:CDS |
| ACATCATTCTTCTTCTGACA+TGG | 0.555858 | 4.3:-5542945 | MS.gene55727:CDS |
| ACAAAGAAACACGACAACCT+CGG | 0.564649 | 4.3:-5543209 | MS.gene55727:CDS |
| CACATTCCGAATCGATTCGA+AGG | 0.565105 | 4.3:+5543092 | None:intergenic |
| AGAGTCCAATGTTCTATACA+AGG | 0.568503 | 4.3:-5542799 | MS.gene55727:CDS |
| ATCTCAAACTCAACCTCATG+AGG | 0.577882 | 4.3:-5543131 | MS.gene55727:CDS |
| ATGCCATACAGGAATCTCAT+GGG | 0.578629 | 4.3:-5542719 | MS.gene55727:CDS |
| TGGTCCCTTGTATAGAACAT+TGG | 0.582832 | 4.3:+5542794 | None:intergenic |
| AGAAACACGACAACCTCGGA+CGG | 0.587887 | 4.3:-5543205 | MS.gene55727:CDS |
| AACATTGGACTCTGTGATGT+TGG | 0.591723 | 4.3:+5542809 | None:intergenic |
| AATATTGTTTCCTCCGTCCG+AGG | 0.597602 | 4.3:+5543192 | None:intergenic |
| GTCCCCATGAGATTCCTGTA+TGG | 0.609295 | 4.3:+5542716 | None:intergenic |
| GAATCGATTCGAAGGAGTCG+TGG | 0.616503 | 4.3:+5543100 | None:intergenic |
| TATGCCATACAGGAATCTCA+TGG | 0.626506 | 4.3:-5542720 | MS.gene55727:CDS |
| ATTGGACTCTGTGATGTTGG+TGG | 0.631848 | 4.3:+5542812 | None:intergenic |
| GAATCATCACCAATTAGCTG+CGG | 0.636521 | 4.3:-5543011 | MS.gene55727:CDS |
| TTGGACTCTGTGATGTTGGT+GGG | 0.640625 | 4.3:+5542813 | None:intergenic |
| GAGTCCAATGTTCTATACAA+GGG | 0.649085 | 4.3:-5542798 | MS.gene55727:CDS |
| ATGGTTGCTGCTTACACAAG+TGG | 0.652558 | 4.3:-5542926 | MS.gene55727:CDS |
| ACGTTAGGGTTACAACAACA+TGG | 0.673570 | 4.3:-5542860 | MS.gene55727:CDS |
| TGCCATACAGGAATCTCATG+GGG | 0.679427 | 4.3:-5542718 | MS.gene55727:CDS |
| GGTGAGAGTTTCCACCAAGG+TGG | 0.686723 | 4.3:-5542905 | MS.gene55727:CDS |
| AGTGGTGAGAGTTTCCACCA+AGG | 0.759976 | 4.3:-5542908 | MS.gene55727:CDS |
| TTAGGGTTACAACAACATGG+TGG | 0.784784 | 4.3:-5542857 | MS.gene55727:CDS |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !!! | ATTGGTTTTGAAGTACATTA+AGG | + | chr4.3:5542789-5542808 | None:intergenic | 25.0% |
| GAACAAATATTATGCCATAC+AGG | - | chr4.3:5543208-5543227 | MS.gene55727:CDS | 30.0% | |
| !! | ATGATTGTTGTTGATGATAG+AGG | + | chr4.3:5542875-5542894 | None:intergenic | 30.0% |
| ACATCATTCTTCTTCTGACA+TGG | - | chr4.3:5542993-5543012 | MS.gene55727:CDS | 35.0% | |
| AGAGTCCAATGTTCTATACA+AGG | - | chr4.3:5543139-5543158 | MS.gene55727:CDS | 35.0% | |
| GAGTCCAATGTTCTATACAA+GGG | - | chr4.3:5543140-5543159 | MS.gene55727:CDS | 35.0% | |
| ! | TTACAACAACATGGTGGAAA+TGG | - | chr4.3:5543087-5543106 | MS.gene55727:CDS | 35.0% |
| AACATTGGACTCTGTGATGT+TGG | + | chr4.3:5543132-5543151 | None:intergenic | 40.0% | |
| AAGGGACCAAATTGAAGAAC+CGG | - | chr4.3:5543158-5543177 | MS.gene55727:CDS | 40.0% | |
| ACAAAGAAACACGACAACCT+CGG | - | chr4.3:5542729-5542748 | MS.gene55727:CDS | 40.0% | |
| ACGTTAGGGTTACAACAACA+TGG | - | chr4.3:5543078-5543097 | MS.gene55727:CDS | 40.0% | |
| ATCTCAAACTCAACCTCATG+AGG | - | chr4.3:5542807-5542826 | MS.gene55727:CDS | 40.0% | |
| ATGCCATACAGGAATCTCAT+GGG | - | chr4.3:5543219-5543238 | MS.gene55727:CDS | 40.0% | |
| GAATCATCACCAATTAGCTG+CGG | - | chr4.3:5542927-5542946 | MS.gene55727:CDS | 40.0% | |
| GACACAATTGCTACATGACT+TGG | - | chr4.3:5543242-5543261 | MS.gene55727:CDS | 40.0% | |
| TATGCCATACAGGAATCTCA+TGG | - | chr4.3:5543218-5543237 | MS.gene55727:CDS | 40.0% | |
| TGGTCCCTTGTATAGAACAT+TGG | + | chr4.3:5543147-5543166 | None:intergenic | 40.0% | |
| TTAGGGTTACAACAACATGG+TGG | - | chr4.3:5543081-5543100 | MS.gene55727:CDS | 40.0% | |
| ! | ACTGAACCGGTTCTTCAATT+TGG | + | chr4.3:5543167-5543186 | None:intergenic | 40.0% |
| ! | CATGAGGTTGAGTTTGAGAT+TGG | + | chr4.3:5542807-5542826 | None:intergenic | 40.0% |
| ! | GTTCAGTACTCTCTTTTGGA+TGG | - | chr4.3:5543180-5543199 | MS.gene55727:CDS | 40.0% |
| ! | TACTCTCTTTTGGATGGTGA+AGG | - | chr4.3:5543186-5543205 | MS.gene55727:CDS | 40.0% |
| !! | TCCAAAAGAGAGTACTGAAC+CGG | + | chr4.3:5543180-5543199 | None:intergenic | 40.0% |
| AATATTGTTTCCTCCGTCCG+AGG | + | chr4.3:5542749-5542768 | None:intergenic | 45.0% | |
| ACGACTCCTTCGAATCGATT+CGG | - | chr4.3:5542840-5542859 | MS.gene55727:CDS | 45.0% | |
| ATTGGACTCTGTGATGTTGG+TGG | + | chr4.3:5543129-5543148 | None:intergenic | 45.0% | |
| TGCCATACAGGAATCTCATG+GGG | - | chr4.3:5543220-5543239 | MS.gene55727:CDS | 45.0% | |
| TGGTGGAAATGGAGTGAGTT+TGG | - | chr4.3:5543098-5543117 | MS.gene55727:CDS | 45.0% | |
| TTGGACTCTGTGATGTTGGT+GGG | + | chr4.3:5543128-5543147 | None:intergenic | 45.0% | |
| ! | ATGGTTGCTGCTTACACAAG+TGG | - | chr4.3:5543012-5543031 | MS.gene55727:CDS | 45.0% |
| ! | GTGGAGTGTCATTGACGTTA+GGG | - | chr4.3:5543064-5543083 | MS.gene55727:CDS | 45.0% |
| !! | ACCGGTTCAGTACTCTCTTT+TGG | - | chr4.3:5543176-5543195 | MS.gene55727:CDS | 45.0% |
| !! | CACATTCCGAATCGATTCGA+AGG | + | chr4.3:5542849-5542868 | None:intergenic | 45.0% |
| AGAAACACGACAACCTCGGA+CGG | - | chr4.3:5542733-5542752 | MS.gene55727:CDS | 50.0% | |
| AGTGGTGAGAGTTTCCACCA+AGG | - | chr4.3:5543030-5543049 | MS.gene55727:CDS | 50.0% | |
| CAAACGCATCCGCAGCTAAT+TGG | + | chr4.3:5542939-5542958 | None:intergenic | 50.0% | |
| CAATTAGCTGCGGATGCGTT+TGG | - | chr4.3:5542937-5542956 | MS.gene55727:CDS | 50.0% | |
| GTCCCCATGAGATTCCTGTA+TGG | + | chr4.3:5543225-5543244 | None:intergenic | 50.0% | |
| ! | CGTGGCTTTTGATCCTCATG+AGG | + | chr4.3:5542823-5542842 | None:intergenic | 50.0% |
| ! | GGTGGAGTGTCATTGACGTT+AGG | - | chr4.3:5543063-5543082 | MS.gene55727:CDS | 50.0% |
| !! | GAATCGATTCGAAGGAGTCG+TGG | + | chr4.3:5542841-5542860 | None:intergenic | 50.0% |
| AGCTGCGGATGCGTTTGGAT+CGG | - | chr4.3:5542942-5542961 | MS.gene55727:CDS | 55.0% | |
| GGTGAGAGTTTCCACCAAGG+TGG | - | chr4.3:5543033-5543052 | MS.gene55727:CDS | 55.0% | |
| AACACGACAACCTCGGACGG+AGG | - | chr4.3:5542736-5542755 | MS.gene55727:CDS | 60.0% | |
| TTCCACCAAGGTGGCAGCAG+TGG | - | chr4.3:5543042-5543061 | MS.gene55727:CDS | 60.0% | |
| CACCAAGGTGGCAGCAGTGG+TGG | - | chr4.3:5543045-5543064 | MS.gene55727:CDS | 65.0% | |
| CACCACTGCTGCCACCTTGG+TGG | + | chr4.3:5543047-5543066 | None:intergenic | 65.0% | |
| CTCCACCACTGCTGCCACCT+TGG | + | chr4.3:5543050-5543069 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.3 | gene | 5542691 | 5543269 | 5542691 | ID=MS.gene55727 |
| chr4.3 | mRNA | 5542691 | 5543269 | 5542691 | ID=MS.gene55727.t1;Parent=MS.gene55727 |
| chr4.3 | exon | 5542691 | 5543269 | 5542691 | ID=MS.gene55727.t1.exon1;Parent=MS.gene55727.t1 |
| chr4.3 | CDS | 5542691 | 5543269 | 5542691 | ID=cds.MS.gene55727.t1;Parent=MS.gene55727.t1 |
| Gene Sequence |
| Protein sequence |
