AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene05996


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05996.t1 MTR_8g098815 98.958 192 0 1 1 192 431 620 5.96e-135 391
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene05996.t1 AT5G41410 38.889 198 76 12 1 192 453 611 5.96e-22 92.8

Find 47 sgRNAs with CRISPR-Local

Find 49 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
ACCGGTTCAGTACTCTCTTT+TGG 0.253768 4.1:-5486313 MS.gene05996:CDS
CAAACGCATCCGCGGCTAAT+TGG 0.275675 4.1:+5486553 None:intergenic
AATGGTGGAAGAAATGTATT+TGG 0.286857 4.1:-5486799 MS.gene05996:CDS
ACTGAACCGGTTCTTCAATT+TGG 0.298745 4.1:+5486325 None:intergenic
CATGAGGTTGAGTTTGAGAT+TGG 0.355537 4.1:+5486685 None:intergenic
CAATTAGCCGCGGATGCGTT+TGG 0.383986 4.1:-5486552 MS.gene05996:CDS
GGTGGAGTGTCATTGACGTT+AGG 0.399182 4.1:-5486426 MS.gene05996:CDS
TTACAACAACATGGTGGAAA+TGG 0.409545 4.1:-5486402 MS.gene05996:CDS
CTCCACCACTGCTGCCACCT+TGG 0.420187 4.1:+5486442 None:intergenic
TGGTGGAAATGGAGTGAGTT+TGG 0.455316 4.1:-5486391 MS.gene05996:CDS
AAATACATTTCTTCCACCAT+TGG 0.455432 4.1:+5486801 None:intergenic
ATGATTGTTGTTGATGATAG+AGG 0.462600 4.1:+5486617 None:intergenic
CACCACTGCTGCCACCTTGG+TGG 0.462654 4.1:+5486445 None:intergenic
AAGGGACCAAATTGAAGAAC+CGG 0.465720 4.1:-5486331 MS.gene05996:CDS
GTGGAGTGTCATTGACGTTA+GGG 0.474230 4.1:-5486425 MS.gene05996:CDS
GAACAAATATTATGCCATAC+AGG 0.476848 4.1:-5486281 MS.gene05996:CDS
CACCAAGGTGGCAGCAGTGG+TGG 0.491693 4.1:-5486444 MS.gene05996:CDS
TCCAAAAGAGAGTACTGAAC+CGG 0.513134 4.1:+5486312 None:intergenic
TTCCACCAAGGTGGCAGCAG+TGG 0.532318 4.1:-5486447 MS.gene05996:CDS
GACACAATTGCTACATGACT+TGG 0.534189 4.1:-5486247 MS.gene05996:CDS
ACGACTCCTTCGAATCGATT+CGG 0.534329 4.1:-5486649 MS.gene05996:CDS
AACACGACAACCTCGGACGG+AGG 0.540366 4.1:-5486753 MS.gene05996:CDS
AGCCGCGGATGCGTTTGGAT+CGG 0.552377 4.1:-5486547 MS.gene05996:CDS
ACATCATTCTTCTTCTGACA+TGG 0.555858 4.1:-5486496 MS.gene05996:CDS
ACAAAGAAACACGACAACCT+CGG 0.564649 4.1:-5486760 MS.gene05996:CDS
CACATTCCGAATCGATTCGA+AGG 0.565105 4.1:+5486643 None:intergenic
AGAGTCCAATGTTCTATACA+AGG 0.568503 4.1:-5486350 MS.gene05996:CDS
ATCTCAAACTCAACCTCATG+AGG 0.577882 4.1:-5486682 MS.gene05996:CDS
ATGCCATACAGGAATCTCAT+GGG 0.578629 4.1:-5486270 MS.gene05996:CDS
TGGTCCCTTGTATAGAACAT+TGG 0.582832 4.1:+5486345 None:intergenic
AGAAACACGACAACCTCGGA+CGG 0.587887 4.1:-5486756 MS.gene05996:CDS
AACATTGGACTCTGTGATGT+TGG 0.591723 4.1:+5486360 None:intergenic
AATATTGTTTCCTCCGTCCG+AGG 0.597602 4.1:+5486743 None:intergenic
GTCCCCATGAGATTCCTGTA+TGG 0.609295 4.1:+5486267 None:intergenic
GAATCGATTCGAAGGAGTCG+TGG 0.616503 4.1:+5486651 None:intergenic
TATGCCATACAGGAATCTCA+TGG 0.626506 4.1:-5486271 MS.gene05996:CDS
ATTGGACTCTGTGATGTTGG+TGG 0.631848 4.1:+5486363 None:intergenic
TTGGACTCTGTGATGTTGGT+GGG 0.640625 4.1:+5486364 None:intergenic
AACCGATCCAAACGCATCCG+CGG 0.640907 4.1:+5486545 None:intergenic
GAGTCCAATGTTCTATACAA+GGG 0.649085 4.1:-5486349 MS.gene05996:CDS
GAATCATCACCAATTAGCCG+CGG 0.649592 4.1:-5486562 MS.gene05996:CDS
ATGGTTGCTGCTTACACAAG+TGG 0.652558 4.1:-5486477 MS.gene05996:CDS
ACGTTAGGGTTACAACAACA+TGG 0.673570 4.1:-5486411 MS.gene05996:CDS
TGCCATACAGGAATCTCATG+GGG 0.679427 4.1:-5486269 MS.gene05996:CDS
GGTGAGAGTTTCCACCAAGG+TGG 0.686723 4.1:-5486456 MS.gene05996:CDS
AGTGGTGAGAGTTTCCACCA+AGG 0.759976 4.1:-5486459 MS.gene05996:CDS
TTAGGGTTACAACAACATGG+TGG 0.784784 4.1:-5486408 MS.gene05996:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! ATTGGTTTTGAAGTACATTA+AGG + chr4.1:5486340-5486359 None:intergenic 25.0%
GAACAAATATTATGCCATAC+AGG - chr4.1:5486759-5486778 MS.gene05996:CDS 30.0%
!! ATGATTGTTGTTGATGATAG+AGG + chr4.1:5486426-5486445 None:intergenic 30.0%
ACATCATTCTTCTTCTGACA+TGG - chr4.1:5486544-5486563 MS.gene05996:CDS 35.0%
AGAGTCCAATGTTCTATACA+AGG - chr4.1:5486690-5486709 MS.gene05996:CDS 35.0%
GAGTCCAATGTTCTATACAA+GGG - chr4.1:5486691-5486710 MS.gene05996:CDS 35.0%
! TTACAACAACATGGTGGAAA+TGG - chr4.1:5486638-5486657 MS.gene05996:CDS 35.0%
AACATTGGACTCTGTGATGT+TGG + chr4.1:5486683-5486702 None:intergenic 40.0%
AAGGGACCAAATTGAAGAAC+CGG - chr4.1:5486709-5486728 MS.gene05996:CDS 40.0%
ACAAAGAAACACGACAACCT+CGG - chr4.1:5486280-5486299 MS.gene05996:CDS 40.0%
ACGTTAGGGTTACAACAACA+TGG - chr4.1:5486629-5486648 MS.gene05996:CDS 40.0%
ATCTCAAACTCAACCTCATG+AGG - chr4.1:5486358-5486377 MS.gene05996:CDS 40.0%
ATGCCATACAGGAATCTCAT+GGG - chr4.1:5486770-5486789 MS.gene05996:CDS 40.0%
GACACAATTGCTACATGACT+TGG - chr4.1:5486793-5486812 MS.gene05996:CDS 40.0%
TATGCCATACAGGAATCTCA+TGG - chr4.1:5486769-5486788 MS.gene05996:CDS 40.0%
TGGTCCCTTGTATAGAACAT+TGG + chr4.1:5486698-5486717 None:intergenic 40.0%
TTAGGGTTACAACAACATGG+TGG - chr4.1:5486632-5486651 MS.gene05996:CDS 40.0%
! ACTGAACCGGTTCTTCAATT+TGG + chr4.1:5486718-5486737 None:intergenic 40.0%
! CATGAGGTTGAGTTTGAGAT+TGG + chr4.1:5486358-5486377 None:intergenic 40.0%
! GTTCAGTACTCTCTTTTGGA+TGG - chr4.1:5486731-5486750 MS.gene05996:CDS 40.0%
! TACTCTCTTTTGGATGGTGA+AGG - chr4.1:5486737-5486756 MS.gene05996:CDS 40.0%
!! TCCAAAAGAGAGTACTGAAC+CGG + chr4.1:5486731-5486750 None:intergenic 40.0%
AATATTGTTTCCTCCGTCCG+AGG + chr4.1:5486300-5486319 None:intergenic 45.0%
ACGACTCCTTCGAATCGATT+CGG - chr4.1:5486391-5486410 MS.gene05996:CDS 45.0%
ATTGGACTCTGTGATGTTGG+TGG + chr4.1:5486680-5486699 None:intergenic 45.0%
GAATCATCACCAATTAGCCG+CGG - chr4.1:5486478-5486497 MS.gene05996:CDS 45.0%
TGCCATACAGGAATCTCATG+GGG - chr4.1:5486771-5486790 MS.gene05996:CDS 45.0%
TGGTGGAAATGGAGTGAGTT+TGG - chr4.1:5486649-5486668 MS.gene05996:CDS 45.0%
TTGGACTCTGTGATGTTGGT+GGG + chr4.1:5486679-5486698 None:intergenic 45.0%
! ATGGTTGCTGCTTACACAAG+TGG - chr4.1:5486563-5486582 MS.gene05996:CDS 45.0%
! GTGGAGTGTCATTGACGTTA+GGG - chr4.1:5486615-5486634 MS.gene05996:CDS 45.0%
!! ACCGGTTCAGTACTCTCTTT+TGG - chr4.1:5486727-5486746 MS.gene05996:CDS 45.0%
!! CACATTCCGAATCGATTCGA+AGG + chr4.1:5486400-5486419 None:intergenic 45.0%
AGAAACACGACAACCTCGGA+CGG - chr4.1:5486284-5486303 MS.gene05996:CDS 50.0%
AGTGGTGAGAGTTTCCACCA+AGG - chr4.1:5486581-5486600 MS.gene05996:CDS 50.0%
GTCCCCATGAGATTCCTGTA+TGG + chr4.1:5486776-5486795 None:intergenic 50.0%
! CGTGGCTTTTGATCCTCATG+AGG + chr4.1:5486374-5486393 None:intergenic 50.0%
! GGTGGAGTGTCATTGACGTT+AGG - chr4.1:5486614-5486633 MS.gene05996:CDS 50.0%
!! GAATCGATTCGAAGGAGTCG+TGG + chr4.1:5486392-5486411 None:intergenic 50.0%
AACCGATCCAAACGCATCCG+CGG + chr4.1:5486498-5486517 None:intergenic 55.0%
CAAACGCATCCGCGGCTAAT+TGG + chr4.1:5486490-5486509 None:intergenic 55.0%
CAATTAGCCGCGGATGCGTT+TGG - chr4.1:5486488-5486507 MS.gene05996:CDS 55.0%
GGTGAGAGTTTCCACCAAGG+TGG - chr4.1:5486584-5486603 MS.gene05996:CDS 55.0%
AACACGACAACCTCGGACGG+AGG - chr4.1:5486287-5486306 MS.gene05996:CDS 60.0%
AGCCGCGGATGCGTTTGGAT+CGG - chr4.1:5486493-5486512 MS.gene05996:CDS 60.0%
TTCCACCAAGGTGGCAGCAG+TGG - chr4.1:5486593-5486612 MS.gene05996:CDS 60.0%
CACCAAGGTGGCAGCAGTGG+TGG - chr4.1:5486596-5486615 MS.gene05996:CDS 65.0%
CACCACTGCTGCCACCTTGG+TGG + chr4.1:5486598-5486617 None:intergenic 65.0%
CTCCACCACTGCTGCCACCT+TGG + chr4.1:5486601-5486620 None:intergenic 65.0%


Chromosome Type Strat End Strand Name
chr4.1 gene 5486242 5486820 5486242 ID=MS.gene05996
chr4.1 mRNA 5486242 5486820 5486242 ID=MS.gene05996.t1;Parent=MS.gene05996
chr4.1 exon 5486242 5486820 5486242 ID=MS.gene05996.t1.exon1;Parent=MS.gene05996.t1
chr4.1 CDS 5486242 5486820 5486242 ID=cds.MS.gene05996.t1;Parent=MS.gene05996.t1
Gene Sequence

>MS.gene05996

ATGGTGGAAGAAATGTATTTGGAAGAAGAGAAAGAGCAACAAAGAAACACGACAACCTCGGACGGAGGAAACAATATTAATCATGAAGAAGATAACCTTAATGTACTTCAAAACCAATCTCAAACTCAACCTCATGAGGATCAAAAGCCACGACTCCTTCGAATCGATTCGGAATGTGTCTCCTCTATCATCAACAACAATCATGATCATAAAAATGATGCTAATAATAAGAATTTGAATCATCACCAATTAGCCGCGGATGCGTTTGGATCGGTTGATATTGATTTTTCATCTTATAATACACATCATTCTTCTTCTGACATGGTTGCTGCTTACACAAGTGGTGAGAGTTTCCACCAAGGTGGCAGCAGTGGTGGAGTGTCATTGACGTTAGGGTTACAACAACATGGTGGAAATGGAGTGAGTTTGGCTTTCCCACCAACATCACAGAGTCCAATGTTCTATACAAGGGACCAAATTGAAGAACCGGTTCAGTACTCTCTTTTGGATGGTGAAGGAACAAATATTATGCCATACAGGAATCTCATGGGGACACAATTGCTACATGACTTGGCATGA

Protein sequence

>MS.gene05996.t1

MVEEMYLEEEKEQQRNTTTSDGGNNINHEEDNLNVLQNQSQTQPHEDQKPRLLRIDSECVSSIINNNHDHKNDANNKNLNHHQLAADAFGSVDIDFSSYNTHHSSSDMVAAYTSGESFHQGGSSGGVSLTLGLQQHGGNGVSLAFPPTSQSPMFYTRDQIEEPVQYSLLDGEGTNIMPYRNLMGTQLLHDLA