AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene55921

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55921.t1 MTR_2g032710 96.711 456 9 1 1 456 14 463 0.0 910
MS.gene55921.t1 MTR_4g094715 74.179 457 107 2 1 456 15 461 0.0 702
MS.gene55921.t1 MTR_1g086390 63.333 390 141 1 59 446 80 469 0.0 548
MS.gene55921.t1 MTR_7g110050 59.765 425 161 4 32 454 46 462 0.0 536
MS.gene55921.t1 MTR_5g034090 46.827 457 203 9 18 444 77 523 1.01e-132 394
MS.gene55921.t1 MTR_8g065010 47.786 429 188 7 41 443 91 509 1.58e-130 387
MS.gene55921.t1 MTR_6g005630 46.253 387 190 6 60 445 45 414 8.23e-119 354
MS.gene55921.t1 MTR_5g033680 41.584 404 217 8 43 441 50 439 7.66e-107 325
MS.gene55921.t1 MTR_6g005630 43.152 387 179 6 60 445 45 391 7.25e-105 318
MS.gene55921.t1 MTR_8g065030 38.406 414 235 7 48 452 53 455 4.22e-95 295
MS.gene55921.t1 MTR_2g103410 39.842 379 215 7 60 435 59 427 1.46e-88 277
MS.gene55921.t1 MTR_1g052315 43.333 360 185 10 54 407 57 403 8.84e-88 275
MS.gene55921.t1 MTR_5g034090 46.131 336 144 7 18 324 77 404 2.98e-87 273
MS.gene55921.t1 MTR_7g091850 41.620 358 196 5 59 413 75 422 5.14e-86 271
MS.gene55921.t1 MTR_7g091840 41.018 334 183 5 49 377 54 378 5.69e-81 255
MS.gene55921.t1 MTR_2g060350 39.394 363 205 7 54 412 68 419 6.32e-81 257
MS.gene55921.t1 MTR_6g028080 36.070 402 220 10 54 438 16 397 1.10e-73 237
MS.gene55921.t1 MTR_2g103650 34.726 383 238 7 60 438 28 402 4.41e-73 236
MS.gene55921.t1 MTR_1g012390 37.018 389 226 9 56 440 24 397 5.59e-72 233
MS.gene55921.t1 MTR_3g054320 35.714 392 226 10 60 446 30 400 2.86e-69 226
MS.gene55921.t1 MTR_3g030540 38.292 363 200 8 61 417 71 415 7.99e-69 226
MS.gene55921.t1 MTR_3g084380 34.916 358 213 6 60 412 21 363 6.10e-67 219
MS.gene55921.t1 MTR_2g049400 35.142 387 234 7 53 438 21 391 2.71e-66 218
MS.gene55921.t1 MTR_2g058670 35.227 352 216 7 59 407 24 366 8.97e-66 217
MS.gene55921.t1 MTR_8g010080 35.770 383 217 10 59 438 30 386 9.98e-65 213
MS.gene55921.t1 MTR_2g058640 36.261 353 211 8 59 407 24 366 1.45e-64 214
MS.gene55921.t1 MTR_4g084050 34.795 365 211 8 60 413 23 371 1.64e-64 213
MS.gene55921.t1 MTR_8g010070 34.536 388 225 11 57 440 28 390 6.89e-64 211
MS.gene55921.t1 MTR_8g010040 35.770 383 214 9 60 437 32 387 8.33e-64 211
MS.gene55921.t1 MTR_2g058840 34.540 359 207 8 59 407 24 364 9.00e-64 212
MS.gene55921.t1 MTR_3g438050 34.232 371 225 7 59 424 26 382 1.07e-63 211
MS.gene55921.t1 MTR_3g084360 34.540 359 215 8 60 413 24 367 3.74e-63 209
MS.gene55921.t1 MTR_1g088160 35.372 376 215 13 59 423 34 392 1.19e-62 209
MS.gene55921.t1 MTR_5g097860 38.037 326 185 7 59 379 24 337 1.79e-62 206
MS.gene55921.t1 MTR_5g043350 33.846 390 233 10 60 442 29 400 3.14e-62 207
MS.gene55921.t1 MTR_0157s0060 34.817 382 221 8 65 440 3 362 5.00e-62 206
MS.gene55921.t1 MTR_4g034020 34.817 382 221 8 65 440 3 362 5.00e-62 206
MS.gene55921.t1 MTR_8g008765 35.780 327 185 5 56 379 27 331 5.99e-62 205
MS.gene55921.t1 MTR_8g010110 33.247 385 229 8 57 438 28 387 1.96e-61 205
MS.gene55921.t1 MTR_4g083660 34.103 390 236 11 53 438 2 374 1.13e-60 202
MS.gene55921.t1 MTR_8g010090 33.506 385 228 9 57 438 28 387 2.01e-60 202
MS.gene55921.t1 MTR_7g035415 36.190 315 177 7 59 368 39 334 7.32e-60 199
MS.gene55921.t1 MTR_7g113910 35.772 369 150 8 59 425 56 339 1.12e-59 200
MS.gene55921.t1 MTR_2g435640 34.262 359 220 7 54 407 47 394 1.64e-58 198
MS.gene55921.t1 MTR_2g103410 37.453 267 157 4 60 324 59 317 4.17e-58 194
MS.gene55921.t1 MTR_8g009810 35.196 358 213 9 60 413 35 377 4.32e-57 194
MS.gene55921.t1 MTR_8g030930 33.983 359 215 9 59 409 26 370 8.34e-57 193
MS.gene55921.t1 MTR_1g013730 32.984 382 225 9 60 438 27 380 2.95e-55 188
MS.gene55921.t1 MTR_4g120320 32.902 386 242 9 55 435 47 420 2.16e-54 187
MS.gene55921.t1 MTR_1g013740 32.552 384 228 9 60 440 35 390 3.44e-54 186
MS.gene55921.t1 MTR_2g029170 34.706 340 203 10 102 438 38 361 1.27e-52 181
MS.gene55921.t1 MTR_8g010050 30.570 386 205 11 57 438 28 354 3.40e-52 179
MS.gene55921.t1 MTR_8g008700 33.746 323 167 8 118 438 13 290 1.12e-47 166
MS.gene55921.t1 MTR_8g077320 29.949 394 199 9 59 437 32 363 2.95e-47 167
MS.gene55921.t1 MTR_5g063430 31.169 308 186 8 59 359 24 312 1.75e-41 150
MS.gene55921.t1 MTR_8g031180 36.538 208 122 3 207 408 70 273 6.20e-39 142
MS.gene55921.t1 MTR_2g100040 31.073 354 178 12 59 406 24 317 1.49e-38 143
MS.gene55921.t1 MTR_4g124980 26.185 401 229 12 48 407 62 436 1.32e-21 97.8
MS.gene55921.t1 MTR_2g042740 25.722 381 236 12 58 407 54 418 5.79e-21 95.5
MS.gene55921.t1 MTR_3g449590 23.844 411 269 10 62 443 49 444 1.89e-17 84.7
MS.gene55921.t1 MTR_5g090160 25.879 313 191 9 65 349 49 348 3.08e-16 80.9
MS.gene55921.t1 MTR_2g435340 25.526 333 195 12 50 348 27 340 5.64e-15 77.0
MS.gene55921.t1 MTR_1g100040 26.214 309 185 11 65 344 61 355 6.20e-15 77.0
MS.gene55921.t1 MTR_8g098840 24.571 350 210 13 62 376 46 376 6.73e-15 77.0
MS.gene55921.t1 MTR_1g100050 30.000 230 131 7 65 276 55 272 1.05e-14 76.3
MS.gene55921.t1 MTR_7g088550 25.649 308 186 10 65 343 55 348 4.21e-14 74.7
MS.gene55921.t1 MTR_1g099850 25.243 309 188 10 65 344 60 354 1.16e-13 73.2
MS.gene55921.t1 MTR_1g100060 27.826 230 136 6 65 276 98 315 2.26e-13 72.4
MS.gene55921.t1 MTR_7g110710 26.006 323 192 10 62 353 45 351 2.76e-13 72.0
MS.gene55921.t1 MTR_7g110710 25.697 323 193 10 62 353 45 351 3.57e-13 71.6
MS.gene55921.t1 MTR_3g096330 29.000 200 116 6 158 344 189 375 5.35e-13 71.6
MS.gene55921.t1 MTR_8g028725 24.058 345 218 11 62 376 57 387 7.07e-13 70.9
MS.gene55921.t1 MTR_7g078680 23.614 415 265 12 65 446 60 455 1.41e-12 69.7
MS.gene55921.t1 MTR_1g086030 25.155 322 195 10 62 353 84 389 2.60e-12 68.9
MS.gene55921.t1 MTR_2g435340 27.230 213 132 6 148 348 74 275 8.81e-12 67.0
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene55921.t1 AT1G48100 71.047 449 123 3 4 448 21 466 0.0 657
MS.gene55921.t1 AT1G02460 61.893 391 144 3 59 444 88 478 0.0 518
MS.gene55921.t1 AT4G01890 56.030 398 170 2 48 440 53 450 1.04e-169 485
MS.gene55921.t1 AT1G56710 54.854 412 178 4 39 444 24 433 1.06e-168 481
MS.gene55921.t1 AT1G60590 45.499 411 191 6 60 443 123 527 2.66e-121 364
MS.gene55921.t1 AT1G10640 45.477 409 193 6 59 443 118 520 4.61e-120 361
MS.gene55921.t1 AT5G14650 45.679 405 204 6 53 454 44 435 6.59e-118 352
MS.gene55921.t1 AT3G26610 41.176 442 233 8 7 440 27 449 1.32e-107 327
MS.gene55921.t1 AT1G70500 41.162 396 220 6 59 452 66 450 5.51e-100 307
MS.gene55921.t1 AT1G80170 41.623 382 211 6 56 434 49 421 9.82e-100 306
MS.gene55921.t1 AT1G23460 41.927 384 206 7 59 438 42 412 3.32e-96 296
MS.gene55921.t1 AT1G23460 41.927 384 206 7 59 438 66 436 4.29e-96 297
MS.gene55921.t1 AT3G57510 41.714 350 189 6 62 407 70 408 1.91e-86 271
MS.gene55921.t1 AT2G41850 41.143 350 191 6 62 407 70 408 1.77e-85 269
MS.gene55921.t1 AT3G07970 38.992 377 212 9 51 423 64 426 1.26e-81 259
MS.gene55921.t1 AT5G14650 43.590 273 142 3 53 324 44 305 1.15e-72 232
MS.gene55921.t1 AT1G80140 36.286 350 205 6 92 438 2 336 9.41e-71 228
MS.gene55921.t1 AT3G15720 37.726 387 214 10 60 439 23 389 1.66e-70 231
MS.gene55921.t1 AT3G15720 37.726 387 214 10 60 439 24 390 1.68e-70 231
MS.gene55921.t1 AT4G35670 34.551 356 215 6 60 412 24 364 1.45e-69 226
MS.gene55921.t1 AT3G15720 37.726 387 211 11 60 439 23 386 5.96e-68 224
MS.gene55921.t1 AT2G43880 35.714 378 221 9 60 433 31 390 4.02e-66 217
MS.gene55921.t1 AT2G43870 37.892 351 201 7 60 407 21 357 5.39e-66 217
MS.gene55921.t1 AT1G80170 40.590 271 151 4 56 324 49 311 9.76e-66 214
MS.gene55921.t1 AT5G44840 33.067 375 225 8 59 426 23 378 1.41e-65 216
MS.gene55921.t1 AT1G05660 33.854 384 236 8 59 440 27 394 4.11e-65 215
MS.gene55921.t1 AT2G43890 35.638 376 224 11 60 433 29 388 3.86e-64 212
MS.gene55921.t1 AT1G05650 33.590 390 241 8 53 440 21 394 6.02e-64 212
MS.gene55921.t1 AT1G78400 35.417 384 232 6 59 439 34 404 6.96e-64 212
MS.gene55921.t1 AT1G65570 36.735 392 226 12 52 438 23 397 1.73e-63 211
MS.gene55921.t1 AT3G59850 36.827 353 203 10 60 407 23 360 1.81e-62 207
MS.gene55921.t1 AT3G59850 36.827 353 203 10 60 407 67 404 3.56e-62 208
MS.gene55921.t1 AT5G27530 33.889 360 208 7 59 413 52 386 4.77e-62 208
MS.gene55921.t1 AT1G17150 36.053 380 226 8 59 434 32 398 2.07e-61 205
MS.gene55921.t1 AT2G33160 35.075 402 240 9 40 433 11 399 2.07e-61 211
MS.gene55921.t1 AT1G02790 36.158 354 199 9 59 407 51 382 6.32e-61 204
MS.gene55921.t1 AT2G43860 35.844 385 226 12 60 440 33 400 9.70e-60 201
MS.gene55921.t1 AT5G48140 34.911 338 203 7 73 407 34 357 1.32e-59 200
MS.gene55921.t1 AT3G07830 36.686 338 198 7 73 407 34 358 2.07e-59 200
MS.gene55921.t1 AT3G07840 35.613 351 209 8 65 412 26 362 3.21e-59 199
MS.gene55921.t1 AT3G15720 36.919 344 191 9 102 439 3 326 5.80e-58 196
MS.gene55921.t1 AT4G13760 33.989 356 215 9 59 407 30 372 8.35e-57 193
MS.gene55921.t1 AT3G07820 35.860 343 203 8 73 412 33 361 1.41e-56 192
MS.gene55921.t1 AT1G43080 34.270 356 214 9 59 407 30 372 1.55e-56 192
MS.gene55921.t1 AT3G14040 37.396 361 208 8 52 408 63 409 3.75e-56 192
MS.gene55921.t1 AT5G17200 31.873 411 230 12 56 440 35 421 6.53e-56 191
MS.gene55921.t1 AT1G43090 34.551 356 213 9 59 407 30 372 7.01e-56 192
MS.gene55921.t1 AT2G43880 37.162 296 172 5 60 354 31 313 1.17e-55 188
MS.gene55921.t1 AT5G44830 31.765 340 202 6 90 426 7 319 1.64e-55 187
MS.gene55921.t1 AT5G39910 30.402 398 197 11 59 439 35 369 2.85e-55 188
MS.gene55921.t1 AT4G18180 35.574 357 209 9 59 407 38 381 3.19e-55 189
MS.gene55921.t1 AT4G32380 32.703 370 224 7 72 433 39 391 3.41e-55 190
MS.gene55921.t1 AT1G43100 34.454 357 212 10 59 407 30 372 2.61e-54 187
MS.gene55921.t1 AT3G07850 37.119 361 208 9 52 408 63 408 1.08e-53 186
MS.gene55921.t1 AT2G15460 34.358 358 211 11 59 407 30 372 1.55e-53 184
MS.gene55921.t1 AT2G26620 33.898 354 216 9 59 407 30 370 2.46e-53 184
MS.gene55921.t1 AT2G15450 34.078 358 212 11 59 407 30 372 5.37e-53 183
MS.gene55921.t1 AT2G40310 33.613 357 213 10 60 407 31 372 1.18e-52 182
MS.gene55921.t1 AT2G15470 33.799 358 213 11 59 407 30 372 1.64e-51 179
MS.gene55921.t1 AT4G32375 36.655 281 151 6 134 407 5 265 1.73e-51 179
MS.gene55921.t1 AT4G32370 35.836 293 166 6 74 358 49 327 3.81e-49 171
MS.gene55921.t1 AT4G35670 32.841 271 168 3 142 412 1 257 6.43e-49 169
MS.gene55921.t1 AT4G32380 35.688 269 152 5 72 335 39 291 6.47e-46 164
MS.gene55921.t1 AT3G42950 28.103 427 257 18 58 451 64 473 9.78e-22 98.2
MS.gene55921.t1 AT5G41870 27.103 321 189 9 59 349 44 349 5.62e-21 95.5
MS.gene55921.t1 AT1G19170 24.672 381 241 11 58 407 85 450 4.12e-20 93.2
MS.gene55921.t1 AT4G33440 26.168 321 190 10 55 344 67 371 7.95e-18 86.3
MS.gene55921.t1 AT3G62110 26.462 325 188 12 62 353 45 351 4.52e-17 84.0
MS.gene55921.t1 AT3G62110 26.462 325 188 12 62 353 45 351 4.52e-17 84.0
MS.gene55921.t1 AT4G23820 26.708 322 182 12 62 349 43 344 1.69e-16 82.0
MS.gene55921.t1 AT2G23900 23.980 392 242 14 65 422 61 430 9.01e-15 76.6
MS.gene55921.t1 AT3G48950 22.899 345 217 10 65 376 50 378 1.24e-14 76.3
MS.gene55921.t1 AT4G23500 24.096 415 265 13 65 447 73 469 2.57e-12 69.3
MS.gene55921.t1 AT3G06770 26.266 316 188 12 62 347 45 345 6.82e-11 64.7
MS.gene55921.t1 AT3G06770 26.266 316 188 12 62 347 46 346 7.47e-11 64.3

Find 106 sgRNAs with CRISPR-Local

Find 192 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCACTTGATTGTTTA+AGG 0.078821 2.3:+57853612 MS.gene55921:CDS
CTTGATCAAATGACTCTAAA+TGG 0.174861 2.3:+57851225 MS.gene55921:CDS
GGAGATTCTCCAGGATTATT+TGG 0.184472 2.3:-57850527 None:intergenic
GAATATGAATGCAATAATTA+AGG 0.201144 2.3:+57853668 MS.gene55921:CDS
GGTACCTTCCTTCCACATTA+TGG 0.224070 2.3:-57850400 None:intergenic
GATAACTCCACAACCGTTTC+AGG 0.228355 2.3:-57853644 None:intergenic
GTTCTTCATGAGCTCAAATT+TGG 0.241900 2.3:+57851569 MS.gene55921:CDS
CCATAAGCATTCCAACAAAA+AGG 0.243640 2.3:-57853569 None:intergenic
TCTCCAGGATTATTTGGATA+TGG 0.275950 2.3:-57850521 None:intergenic
GCAGCTTGTGCAGTTGATTC+AGG 0.281433 2.3:+57850638 MS.gene55921:CDS
GGATTATTTGGATATGGATA+TGG 0.286489 2.3:-57850515 None:intergenic
GTGCTCCTACCCACGGAATT+AGG 0.301522 2.3:+57851930 MS.gene55921:CDS
CAATTCCATGTGAAATTTGA+TGG 0.330222 2.3:+57851624 MS.gene55921:CDS
TCTGCCTCAGGCCAACAATT+TGG 0.335193 2.3:-57851171 None:intergenic
ATCTTCCTTGCAAACCTCAT+AGG 0.364129 2.3:+57851286 MS.gene55921:CDS
AGTTTCTTACATTCTCCATT+TGG 0.366739 2.3:-57853331 None:intergenic
TATCTTGTTTATAGATGATA+AGG 0.370877 2.3:+57851547 MS.gene55921:intron
TCCTCCACTTGATTGTTTAA+GGG 0.382641 2.3:+57853613 MS.gene55921:CDS
TTCGACAGCATTGGAAGCCT+TGG 0.386798 2.3:+57853173 MS.gene55921:intron
GGCCAACAATTTGGTCCATC+TGG 0.390506 2.3:-57851162 None:intergenic
AGCTCAAATTTGGTGTTAAG+AGG 0.391670 2.3:+57851579 MS.gene55921:CDS
TGACTGAGTTGCGGACTGTT+AGG 0.400859 2.3:-57853226 None:intergenic
ATTATTATTATTACCCTATG+AGG 0.401164 2.3:-57851300 None:intergenic
TCAGTATTGGGACTAAGTTT+TGG 0.402102 2.3:-57851687 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.404860 2.3:+57851869 MS.gene55921:intron
TTATTTACCATTGCTTATCA+AGG 0.419053 2.3:-57851754 None:intergenic
AAGTGGATTCCATCAGTATT+GGG 0.420148 2.3:-57851699 None:intergenic
AATATGAATGCAATAATTAA+GGG 0.424968 2.3:+57853669 MS.gene55921:CDS
CATCCCTTGTGTTTCCTAAG+TGG 0.427078 2.3:-57851716 None:intergenic
TTGAAATATATGTAGATTGA+TGG 0.430307 2.3:+57851126 MS.gene55921:intron
GGAGGTACAGGATCAGTAAC+AGG 0.441303 2.3:+57853290 MS.gene55921:CDS
TAAGTGGATTCCATCAGTAT+TGG 0.443227 2.3:-57851700 None:intergenic
ACTAGGAGATGTAGGAGGAT+TGG 0.444391 2.3:-57850480 None:intergenic
TTACATATACTGCTGATGTT+TGG 0.447172 2.3:-57853400 None:intergenic
AACATTTGACAACATCCAAA+TGG 0.452717 2.3:+57853316 MS.gene55921:CDS
TCAAATTTCACATGGAATTG+AGG 0.453281 2.3:-57851621 None:intergenic
TTGATCAAATGACTCTAAAT+GGG 0.457341 2.3:+57851226 MS.gene55921:CDS
GTAGATTGATGGTACTTTGA+TGG 0.462628 2.3:+57851137 MS.gene55921:intron
GATTGCATTTCAATTGGTCC+TGG 0.464171 2.3:+57851875 MS.gene55921:CDS
GTAAGATCATTTGGAGCAGT+TGG 0.464835 2.3:+57850569 MS.gene55921:CDS
CACCAGATGGACCAAATTGT+TGG 0.467082 2.3:+57851160 MS.gene55921:CDS
CTTACTTGAAATACCAGTCC+TGG 0.467508 2.3:-57850728 None:intergenic
GGATTGGAAGAAGGTGGTGA+AGG 0.469757 2.3:-57850464 None:intergenic
AAAACATGGCAAGGAGGTAC+AGG 0.486274 2.3:+57853278 MS.gene55921:CDS
TCAAGTAATTCACCTTCATA+AGG 0.493532 2.3:-57853542 None:intergenic
TCACTACAAGCAAAGTGTAT+TGG 0.494006 2.3:-57853473 None:intergenic
TATCCATATCCAAATAATCC+TGG 0.495114 2.3:+57850518 MS.gene55921:CDS
GGATATGGATATGGATCAGA+TGG 0.495462 2.3:-57850506 None:intergenic
AGGTGAGCCTGAAACGGTTG+TGG 0.500843 2.3:+57853637 MS.gene55921:CDS
TGGTCCATGCAAACCAGGAC+TGG 0.506011 2.3:+57850715 MS.gene55921:CDS
AATACCAGTCCTGGTTTGCA+TGG 0.514150 2.3:-57850719 None:intergenic
GATGTAGGAGGATTGGAAGA+AGG 0.516798 2.3:-57850473 None:intergenic
TCTTCCTTGCAAACCTCATA+GGG 0.518124 2.3:+57851287 MS.gene55921:CDS
GGTGAAGGGTCACTAGGTGC+TGG 0.521610 2.3:-57850449 None:intergenic
TAATCCTGGAGAATCTCCTT+CGG 0.522281 2.3:+57850532 MS.gene55921:CDS
AAACTTAGTCCCAATACTGA+TGG 0.523177 2.3:+57851690 MS.gene55921:CDS
AATACTGATGGAATCCACTT+AGG 0.526638 2.3:+57851702 MS.gene55921:CDS
CAAATGACTCTAAATGGGAC+AGG 0.527686 2.3:+57851231 MS.gene55921:CDS
CCACTTGATTGTTTAAGGGA+AGG 0.528218 2.3:+57853617 MS.gene55921:CDS
GATGGAACACTAGGAGATGT+AGG 0.530462 2.3:-57850488 None:intergenic
AATGGGACAGGAATTATTGA+AGG 0.532465 2.3:+57851243 MS.gene55921:CDS
GATTGGAAGAAGGTGGTGAA+GGG 0.533569 2.3:-57850463 None:intergenic
AAGGGAAGGTGAGCCTGAAA+CGG 0.547901 2.3:+57853631 MS.gene55921:CDS
GTCTATAAAACACAAGCCAT+TGG 0.548487 2.3:-57851203 None:intergenic
TCATTTGGAGCAGTTGGAGA+TGG 0.548866 2.3:+57850575 MS.gene55921:CDS
CTACAAGCAAAGTGTATTGG+AGG 0.549583 2.3:-57853470 None:intergenic
ACAGGAATTATTGAAGGCAA+TGG 0.554020 2.3:+57851249 MS.gene55921:CDS
GAAGGTGGTGAAGGGTCACT+AGG 0.554808 2.3:-57850455 None:intergenic
GCTCAAATTTGGTGTTAAGA+GGG 0.555873 2.3:+57851580 MS.gene55921:CDS
GTAGGAGGATTGGAAGAAGG+TGG 0.559194 2.3:-57850470 None:intergenic
TTAGGAAACACAAGGGATGT+TGG 0.561840 2.3:+57851720 MS.gene55921:CDS
TATAATTCCTTGATAAGCAA+TGG 0.563522 2.3:+57851747 MS.gene55921:CDS
GTGAAATTTGATGGTTGTCA+AGG 0.563909 2.3:+57851633 MS.gene55921:CDS
ATGTGACATACCTAATTCCG+TGG 0.569578 2.3:-57851940 None:intergenic
CAATCCGAAGGAGATTCTCC+AGG 0.577573 2.3:-57850536 None:intergenic
ACATCGAAAACGCAATCCGA+AGG 0.582946 2.3:-57850548 None:intergenic
TATGGATCAGATGGAACACT+AGG 0.584081 2.3:-57850497 None:intergenic
TCATCCATAATGTGGAAGGA+AGG 0.585473 2.3:+57850396 MS.gene55921:CDS
GTTACTTGTGCTCCTACCCA+CGG 0.586560 2.3:+57851923 MS.gene55921:CDS
ATTACCCTATGAGGTTTGCA+AGG 0.592983 2.3:-57851291 None:intergenic
CTAAGAATCAAAACATGGCA+AGG 0.597482 2.3:+57853269 MS.gene55921:CDS
AAGGCAATGGAGAACAATGG+TGG 0.608620 2.3:+57851262 MS.gene55921:CDS
ACATTCACATCTGAACATCC+AGG 0.609157 2.3:-57851893 None:intergenic
CGTGGGAATTGTGTACTCCA+AGG 0.610752 2.3:-57853190 None:intergenic
TGTTCAGATGTGAATGTGGA+TGG 0.618209 2.3:+57851899 MS.gene55921:CDS
GTCATAAAAGAATCAGACAA+TGG 0.619796 2.3:+57853245 MS.gene55921:CDS
TCCCTTAAACAATCAAGTGG+AGG 0.624213 2.3:-57853614 None:intergenic
AGGCAATGGAGAACAATGGT+GGG 0.624461 2.3:+57851263 MS.gene55921:CDS
GACAACCATCAAATTTCACA+TGG 0.633858 2.3:-57851629 None:intergenic
AGGCAGACAGTAAAAGCCAA+TGG 0.642020 2.3:+57851187 MS.gene55921:CDS
CCTTCCCTTAAACAATCAAG+TGG 0.643008 2.3:-57853617 None:intergenic
GGTACTTTGATGGCACCAGA+TGG 0.645534 2.3:+57851147 MS.gene55921:CDS
TGGACCAAATTGTTGGCCTG+AGG 0.650727 2.3:+57851167 MS.gene55921:CDS
ACATACCTAATTCCGTGGGT+AGG 0.654879 2.3:-57851935 None:intergenic
GAATCCACTTAGGAAACACA+AGG 0.655820 2.3:+57851712 MS.gene55921:CDS
TGGATGTTCAGATGTGAATG+TGG 0.656226 2.3:+57851895 MS.gene55921:CDS
TAGGTTTGAGACACATGCGT+GGG 0.660709 2.3:-57853207 None:intergenic
TTGAAGGCAATGGAGAACAA+TGG 0.660810 2.3:+57851259 MS.gene55921:CDS
TTAGGTTTGAGACACATGCG+TGG 0.673355 2.3:-57853208 None:intergenic
CTCACTGTAGGGCTAACACA+TGG 0.682946 2.3:-57851432 None:intergenic
CAGCTGCATTTAGAGCAGCA+TGG 0.685070 2.3:+57850612 MS.gene55921:CDS
TGTGACATACCTAATTCCGT+GGG 0.685322 2.3:-57851939 None:intergenic
GGAACACTAGGAGATGTAGG+AGG 0.693701 2.3:-57850485 None:intergenic
ATGGACTAAGAATCAAAACA+TGG 0.703229 2.3:+57853264 MS.gene55921:CDS
AGAATCAAAACATGGCAAGG+AGG 0.705826 2.3:+57853272 MS.gene55921:CDS
AATCCACTTAGGAAACACAA+GGG 0.771508 2.3:+57851713 MS.gene55921:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! CAAGAAAAATAAATAAATAA+TGG + chr2.3:57852197-57852216 MS.gene55921:intron 10.0%
!! CAATTAATTATGATTAATTT+AGG + chr2.3:57852001-57852020 MS.gene55921:intron 10.0%
!! TCATAAGAAAAAAAATTATA+AGG - chr2.3:57851525-57851544 None:intergenic 10.0%
!!! AATATAGAATTTTTATTCTA+TGG + chr2.3:57851801-57851820 MS.gene55921:intron 10.0%
!!! AATGTTACATTTTATTTAAT+AGG + chr2.3:57851848-57851867 MS.gene55921:intron 10.0%
!!! AGTTTTTTTTTTTTTTTTTC+AGG - chr2.3:57852833-57852852 None:intergenic 10.0%
!!! TTTTTTCTTCAAATTTATTT+TGG + chr2.3:57853002-57853021 MS.gene55921:intron 10.0%
!! AACTTAATCTTAAAAACAAT+TGG - chr2.3:57852655-57852674 None:intergenic 15.0%
!! TCATAAAATGATTACATAAT+TGG - chr2.3:57852946-57852965 None:intergenic 15.0%
!!! AATTAAATTTTACACAATGA+TGG - chr2.3:57851083-57851102 None:intergenic 15.0%
!!! ATTTTAAAACAGTAATTTTC+AGG - chr2.3:57850677-57850696 None:intergenic 15.0%
!!! TAAAACTGATTTTATATGTT+TGG + chr2.3:57852554-57852573 MS.gene55921:intron 15.0%
!!! TCTGTTATTTTAGTTAAATT+GGG + chr2.3:57851967-57851986 MS.gene55921:intron 15.0%
!!! TTCTGTTATTTTAGTTAAAT+TGG + chr2.3:57851966-57851985 MS.gene55921:intron 15.0%
!!! TTTTTTTTTCAGGTTTATTT+TGG - chr2.3:57852823-57852842 None:intergenic 15.0%
!! ACCTTGTTAGTTAATTATAT+AGG - chr2.3:57851107-57851126 None:intergenic 20.0%
!! ATTATTATTATTACCCTATG+AGG - chr2.3:57851303-57851322 None:intergenic 20.0%
!! TCCTATATAATTAACTAACA+AGG + chr2.3:57851103-57851122 MS.gene55921:intron 20.0%
!! TTGAAATATATGTAGATTGA+TGG + chr2.3:57851126-57851145 MS.gene55921:intron 20.0%
!!! ACATCTTCTTAGAATTATTT+TGG + chr2.3:57853035-57853054 MS.gene55921:intron 20.0%
!!! ATCACAGTTTATTATTTTAG+AGG + chr2.3:57852529-57852548 MS.gene55921:intron 20.0%
!!! CTTTAAATTTTAGACAAATG+TGG + chr2.3:57850796-57850815 MS.gene55921:intron 20.0%
!!! TAAATTTCCTTTTATGTTCA+CGG + chr2.3:57850760-57850779 MS.gene55921:intron 20.0%
!!! TATCTTGTTTATAGATGATA+AGG + chr2.3:57851547-57851566 MS.gene55921:intron 20.0%
! AAAAAGAAACTGTGATGTAA+TGG - chr2.3:57851482-57851501 None:intergenic 25.0%
! ACTATTGTCACATTAATGAT+TGG - chr2.3:57852336-57852355 None:intergenic 25.0%
! ATCTCCTACAGAAAAATAAA+GGG + chr2.3:57853428-57853447 MS.gene55921:CDS 25.0%
! GTGATCATTTGATGTTAAAA+AGG - chr2.3:57852246-57852265 None:intergenic 25.0%
! TATAATTCCTTGATAAGCAA+TGG + chr2.3:57851747-57851766 MS.gene55921:CDS 25.0%
! TATCTCCTACAGAAAAATAA+AGG + chr2.3:57853427-57853446 MS.gene55921:CDS 25.0%
! TTATTTACCATTGCTTATCA+AGG - chr2.3:57851757-57851776 None:intergenic 25.0%
! TTGATCAAATGACTCTAAAT+GGG + chr2.3:57851226-57851245 MS.gene55921:CDS 25.0%
! TTTAACATCAAATGATCACA+GGG + chr2.3:57852247-57852266 MS.gene55921:intron 25.0%
!! ATTGAACTTTTACCTTATGA+AGG + chr2.3:57853530-57853549 MS.gene55921:CDS 25.0%
!! CTTTTGATAAACAGTAGAAT+TGG - chr2.3:57853370-57853389 None:intergenic 25.0%
!! TGAATATTTTACTCACTGTA+GGG - chr2.3:57851446-57851465 None:intergenic 25.0%
!! TTTTAACATCAAATGATCAC+AGG + chr2.3:57852246-57852265 MS.gene55921:intron 25.0%
!!! AGAAGTTGTTTTATGTTGTA+TGG + chr2.3:57852913-57852932 MS.gene55921:intron 25.0%
!!! TTGCCTTTTGAAATGATTTT+TGG - chr2.3:57852098-57852117 None:intergenic 25.0%
AAACTGTGATGTAATGGTTA+AGG - chr2.3:57851476-57851495 None:intergenic 30.0%
AACATTTGACAACATCCAAA+TGG + chr2.3:57853316-57853335 MS.gene55921:CDS 30.0%
AGTTTCTTACATTCTCCATT+TGG - chr2.3:57853334-57853353 None:intergenic 30.0%
ATAAAAGTTTGCAGCGTAAT+TGG + chr2.3:57851039-57851058 MS.gene55921:intron 30.0%
ATATATACACCCAAATTCTG+TGG + chr2.3:57852071-57852090 MS.gene55921:intron 30.0%
CAATTCCATGTGAAATTTGA+TGG + chr2.3:57851624-57851643 MS.gene55921:CDS 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr2.3:57853571-57853590 None:intergenic 30.0%
CTTGATCAAATGACTCTAAA+TGG + chr2.3:57851225-57851244 MS.gene55921:CDS 30.0%
CTTGCTACTCAAAATTCTAT+AGG - chr2.3:57853114-57853133 None:intergenic 30.0%
GACTATAGTACTTTAACAGT+TGG + chr2.3:57852297-57852316 MS.gene55921:intron 30.0%
GGATTATTTGGATATGGATA+TGG - chr2.3:57850518-57850537 None:intergenic 30.0%
GGTCCAAAAATCATTTCAAA+AGG + chr2.3:57852092-57852111 MS.gene55921:intron 30.0%
GTCATAAAAGAATCAGACAA+TGG + chr2.3:57853245-57853264 MS.gene55921:CDS 30.0%
TAAGCTACAAACTTAGTTGA+TGG + chr2.3:57852037-57852056 MS.gene55921:intron 30.0%
TATCCATATCCAAATAATCC+TGG + chr2.3:57850518-57850537 MS.gene55921:CDS 30.0%
TCAAATTTCACATGGAATTG+AGG - chr2.3:57851624-57851643 None:intergenic 30.0%
TCAAGTAATTCACCTTCATA+AGG - chr2.3:57853545-57853564 None:intergenic 30.0%
TTACATATACTGCTGATGTT+TGG - chr2.3:57853403-57853422 None:intergenic 30.0%
! ATCACTTCTACTATCTTTTC+TGG + chr2.3:57850695-57850714 MS.gene55921:CDS 30.0%
! ATGGACTAAGAATCAAAACA+TGG + chr2.3:57853264-57853283 MS.gene55921:CDS 30.0%
! ATTCTTTTATGACTGAGTTG+CGG - chr2.3:57853238-57853257 None:intergenic 30.0%
! CTGAATATTTTACTCACTGT+AGG - chr2.3:57851447-57851466 None:intergenic 30.0%
! GATTCTTTTCATCCATAATG+TGG + chr2.3:57850388-57850407 MS.gene55921:CDS 30.0%
! GTTTTCGATGTAAGATCATT+TGG + chr2.3:57850560-57850579 MS.gene55921:CDS 30.0%
! TTTTCATTTCAGAGTCCAAA+TGG + chr2.3:57851393-57851412 MS.gene55921:intron 30.0%
!! ATTTTTGGACCACAGAATTT+GGG - chr2.3:57852083-57852102 None:intergenic 30.0%
!! TTTTCTTTGGTTTCTTGTGA+TGG - chr2.3:57850424-57850443 None:intergenic 30.0%
!!! ACGATTGTGGTTTTAAATTG+TGG + chr2.3:57850906-57850925 MS.gene55921:intron 30.0%
!!! TGTACCCTTTATTTTTCTGT+AGG - chr2.3:57853435-57853454 None:intergenic 30.0%
!!! TTACATGTGATGTGCTATTT+TGG + chr2.3:57853086-57853105 MS.gene55921:intron 30.0%
!!! TTACCTTGGGACTAATTTTT+TGG + chr2.3:57852412-57852431 MS.gene55921:intron 30.0%
AAACTTAGTCCCAATACTGA+TGG + chr2.3:57851690-57851709 MS.gene55921:CDS 35.0%
AAGGTAATAGATTGCTACCT+AGG - chr2.3:57852399-57852418 None:intergenic 35.0%
AATACTGATGGAATCCACTT+AGG + chr2.3:57851702-57851721 MS.gene55921:CDS 35.0%
AATCCACTTAGGAAACACAA+GGG + chr2.3:57851713-57851732 MS.gene55921:CDS 35.0%
AATGGGACAGGAATTATTGA+AGG + chr2.3:57851243-57851262 MS.gene55921:CDS 35.0%
ACAGGAATTATTGAAGGCAA+TGG + chr2.3:57851249-57851268 MS.gene55921:CDS 35.0%
AGGTAGCAATCTATTACCTT+GGG + chr2.3:57852399-57852418 MS.gene55921:intron 35.0%
AGTTATTACACTCACACTAC+AGG - chr2.3:57852143-57852162 None:intergenic 35.0%
CCAAATGGAAAAACTGTATC+AGG + chr2.3:57851408-57851427 MS.gene55921:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr2.3:57853572-57853591 None:intergenic 35.0%
CTAAGAATCAAAACATGGCA+AGG + chr2.3:57853269-57853288 MS.gene55921:CDS 35.0%
GACAACCATCAAATTTCACA+TGG - chr2.3:57851632-57851651 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr2.3:57851869-57851888 MS.gene55921:intron 35.0%
GTCTATAAAACACAAGCCAT+TGG - chr2.3:57851206-57851225 None:intergenic 35.0%
GTGAGTGTAATAACTCTTGA+AGG + chr2.3:57852148-57852167 MS.gene55921:intron 35.0%
GTTCTTCATGAGCTCAAATT+TGG + chr2.3:57851569-57851588 MS.gene55921:CDS 35.0%
TAGGTAGCAATCTATTACCT+TGG + chr2.3:57852398-57852417 MS.gene55921:intron 35.0%
TCACTACAAGCAAAGTGTAT+TGG - chr2.3:57853476-57853495 None:intergenic 35.0%
TCCTCCACTTGATTGTTTAA+GGG + chr2.3:57853613-57853632 MS.gene55921:CDS 35.0%
TCTCCAGGATTATTTGGATA+TGG - chr2.3:57850524-57850543 None:intergenic 35.0%
TGTCCAAAAAATTAGTCCCA+AGG - chr2.3:57852418-57852437 None:intergenic 35.0%
TTCCTCCACTTGATTGTTTA+AGG + chr2.3:57853612-57853631 MS.gene55921:CDS 35.0%
! AAGTGGATTCCATCAGTATT+GGG - chr2.3:57851702-57851721 None:intergenic 35.0%
! CCTGATACAGTTTTTCCATT+TGG - chr2.3:57851411-57851430 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr2.3:57853569-57853588 MS.gene55921:CDS 35.0%
! CGCTGCAAACTTTTATATTG+CGG - chr2.3:57851035-57851054 None:intergenic 35.0%
! CTTTTCATCCATAATGTGGA+AGG + chr2.3:57850392-57850411 MS.gene55921:CDS 35.0%
! GCAATTTAGAATCACGATTG+TGG + chr2.3:57850893-57850912 MS.gene55921:intron 35.0%
! GTAGATTGATGGTACTTTGA+TGG + chr2.3:57851137-57851156 MS.gene55921:intron 35.0%
! GTGAAATTTGATGGTTGTCA+AGG + chr2.3:57851633-57851652 MS.gene55921:CDS 35.0%
! TAAGTGGATTCCATCAGTAT+TGG - chr2.3:57851703-57851722 None:intergenic 35.0%
!! ACTAAGTTTTGGTGCAGAAA+TGG - chr2.3:57851679-57851698 None:intergenic 35.0%
!! AGCTCAAATTTGGTGTTAAG+AGG + chr2.3:57851579-57851598 MS.gene55921:CDS 35.0%
!! GATTTTTGGACCACAGAATT+TGG - chr2.3:57852084-57852103 None:intergenic 35.0%
!! GCTCAAATTTGGTGTTAAGA+GGG + chr2.3:57851580-57851599 MS.gene55921:CDS 35.0%
!! TACTTGATGACCCTTTTTGT+TGG + chr2.3:57853558-57853577 MS.gene55921:CDS 35.0%
!! TCAGTATTGGGACTAAGTTT+TGG - chr2.3:57851690-57851709 None:intergenic 35.0%
AACGCAACCGTGAACATAAA+AGG - chr2.3:57850770-57850789 None:intergenic 40.0%
ACATTCACATCTGAACATCC+AGG - chr2.3:57851896-57851915 None:intergenic 40.0%
AGAATCAAAACATGGCAAGG+AGG + chr2.3:57853272-57853291 MS.gene55921:CDS 40.0%
ATCTTCCTTGCAAACCTCAT+AGG + chr2.3:57851286-57851305 MS.gene55921:CDS 40.0%
ATGTGACATACCTAATTCCG+TGG - chr2.3:57851943-57851962 None:intergenic 40.0%
ATTACCCTATGAGGTTTGCA+AGG - chr2.3:57851294-57851313 None:intergenic 40.0%
CAAATGACTCTAAATGGGAC+AGG + chr2.3:57851231-57851250 MS.gene55921:CDS 40.0%
CCACTTGATTGTTTAAGGGA+AGG + chr2.3:57853617-57853636 MS.gene55921:CDS 40.0%
CCTTCCCTTAAACAATCAAG+TGG - chr2.3:57853620-57853639 None:intergenic 40.0%
CTACAAGCAAAGTGTATTGG+AGG - chr2.3:57853473-57853492 None:intergenic 40.0%
CTGTCGAAATCACATCAGTA+AGG - chr2.3:57853161-57853180 None:intergenic 40.0%
CTTACTTGAAATACCAGTCC+TGG - chr2.3:57850731-57850750 None:intergenic 40.0%
CTTCAAAATCCTTTACGCTG+CGG - chr2.3:57850951-57850970 None:intergenic 40.0%
GAATCCACTTAGGAAACACA+AGG + chr2.3:57851712-57851731 MS.gene55921:CDS 40.0%
GATTGCATTTCAATTGGTCC+TGG + chr2.3:57851875-57851894 MS.gene55921:CDS 40.0%
GCACATCACATGTAACTTCT+AGG - chr2.3:57853081-57853100 None:intergenic 40.0%
GGAGATTCTCCAGGATTATT+TGG - chr2.3:57850530-57850549 None:intergenic 40.0%
GGATATGGATATGGATCAGA+TGG - chr2.3:57850509-57850528 None:intergenic 40.0%
TAATCCTGGAGAATCTCCTT+CGG + chr2.3:57850532-57850551 MS.gene55921:CDS 40.0%
TATGGATCAGATGGAACACT+AGG - chr2.3:57850500-57850519 None:intergenic 40.0%
TCATCCATAATGTGGAAGGA+AGG + chr2.3:57850396-57850415 MS.gene55921:CDS 40.0%
TCCCTTAAACAATCAAGTGG+AGG - chr2.3:57853617-57853636 None:intergenic 40.0%
TCTTCCTTGCAAACCTCATA+GGG + chr2.3:57851287-57851306 MS.gene55921:CDS 40.0%
TGATGTGATTTCGACAGCAT+TGG + chr2.3:57853164-57853183 MS.gene55921:intron 40.0%
TGGATGTTCAGATGTGAATG+TGG + chr2.3:57851895-57851914 MS.gene55921:CDS 40.0%
TGTGACATACCTAATTCCGT+GGG - chr2.3:57851942-57851961 None:intergenic 40.0%
TGTGTACTTCTCATCAACTG+TGG - chr2.3:57852748-57852767 None:intergenic 40.0%
TGTTCAGATGTGAATGTGGA+TGG + chr2.3:57851899-57851918 MS.gene55921:CDS 40.0%
TTAGACAAATGTGGCTGATG+TGG + chr2.3:57850805-57850824 MS.gene55921:intron 40.0%
TTAGGAAACACAAGGGATGT+TGG + chr2.3:57851720-57851739 MS.gene55921:CDS 40.0%
TTGAAGGCAATGGAGAACAA+TGG + chr2.3:57851259-57851278 MS.gene55921:CDS 40.0%
! GATGAGAAGTACACACTTAG+TGG + chr2.3:57852753-57852772 MS.gene55921:intron 40.0%
! GTAAGATCATTTGGAGCAGT+TGG + chr2.3:57850569-57850588 MS.gene55921:CDS 40.0%
AAGGCAATGGAGAACAATGG+TGG + chr2.3:57851262-57851281 MS.gene55921:CDS 45.0%
ACATACCTAATTCCGTGGGT+AGG - chr2.3:57851938-57851957 None:intergenic 45.0%
ACATCGAAAACGCAATCCGA+AGG - chr2.3:57850551-57850570 None:intergenic 45.0%
ACTAGGAGATGTAGGAGGAT+TGG - chr2.3:57850483-57850502 None:intergenic 45.0%
AGGCAATGGAGAACAATGGT+GGG + chr2.3:57851263-57851282 MS.gene55921:CDS 45.0%
AGGCAGACAGTAAAAGCCAA+TGG + chr2.3:57851187-57851206 MS.gene55921:CDS 45.0%
ATAGATTGCTACCTAGGAGC+AGG - chr2.3:57852393-57852412 None:intergenic 45.0%
CACCAGATGGACCAAATTGT+TGG + chr2.3:57851160-57851179 MS.gene55921:CDS 45.0%
CATCCCTTGTGTTTCCTAAG+TGG - chr2.3:57851719-57851738 None:intergenic 45.0%
GATAACTCCACAACCGTTTC+AGG - chr2.3:57853647-57853666 None:intergenic 45.0%
GATGGAACACTAGGAGATGT+AGG - chr2.3:57850491-57850510 None:intergenic 45.0%
GATGTAGGAGGATTGGAAGA+AGG - chr2.3:57850476-57850495 None:intergenic 45.0%
GATTGGAAGAAGGTGGTGAA+GGG - chr2.3:57850466-57850485 None:intergenic 45.0%
GGCTGCAATTATGGTTGCAA+AGG + chr2.3:57850826-57850845 MS.gene55921:intron 45.0%
GGTACCTTCCTTCCACATTA+TGG - chr2.3:57850403-57850422 None:intergenic 45.0%
GTGGTCTGCAACAGTAATAG+AGG + chr2.3:57850925-57850944 MS.gene55921:intron 45.0%
TAGATTGCTACCTAGGAGCA+GGG - chr2.3:57852392-57852411 None:intergenic 45.0%
! AAAACATGGCAAGGAGGTAC+AGG + chr2.3:57853278-57853297 MS.gene55921:CDS 45.0%
! AATACCAGTCCTGGTTTGCA+TGG - chr2.3:57850722-57850741 None:intergenic 45.0%
! CTGCAGAAACTTTTACGCTG+TGG + chr2.3:57850849-57850868 MS.gene55921:intron 45.0%
! TAGGTTTGAGACACATGCGT+GGG - chr2.3:57853210-57853229 None:intergenic 45.0%
! TCATTTGGAGCAGTTGGAGA+TGG + chr2.3:57850575-57850594 MS.gene55921:CDS 45.0%
! TTAGGTTTGAGACACATGCG+TGG - chr2.3:57853211-57853230 None:intergenic 45.0%
!! GGTGCTGGAGAAGTTTTCTT+TGG - chr2.3:57850437-57850456 None:intergenic 45.0%
!! TTTTCTGGTCCATGCAAACC+AGG + chr2.3:57850710-57850729 MS.gene55921:CDS 45.0%
AAGGGAAGGTGAGCCTGAAA+CGG + chr2.3:57853631-57853650 MS.gene55921:CDS 50.0%
AGATTGCTACCTAGGAGCAG+GGG - chr2.3:57852391-57852410 None:intergenic 50.0%
CAATCCGAAGGAGATTCTCC+AGG - chr2.3:57850539-57850558 None:intergenic 50.0%
CGTGGGAATTGTGTACTCCA+AGG - chr2.3:57853193-57853212 None:intergenic 50.0%
CTCACTGTAGGGCTAACACA+TGG - chr2.3:57851435-57851454 None:intergenic 50.0%
GGAACACTAGGAGATGTAGG+AGG - chr2.3:57850488-57850507 None:intergenic 50.0%
GGATTGGAAGAAGGTGGTGA+AGG - chr2.3:57850467-57850486 None:intergenic 50.0%
GGCCAACAATTTGGTCCATC+TGG - chr2.3:57851165-57851184 None:intergenic 50.0%
GGCTGATGTGGCTGCAATTA+TGG + chr2.3:57850817-57850836 MS.gene55921:intron 50.0%
GTAGGAGGATTGGAAGAAGG+TGG - chr2.3:57850473-57850492 None:intergenic 50.0%
GTTACTTGTGCTCCTACCCA+CGG + chr2.3:57851923-57851942 MS.gene55921:CDS 50.0%
TAATAGAGGCCGCAGCGTAA+AGG + chr2.3:57850939-57850958 MS.gene55921:intron 50.0%
TCTGCCTCAGGCCAACAATT+TGG - chr2.3:57851174-57851193 None:intergenic 50.0%
! CAGCTGCATTTAGAGCAGCA+TGG + chr2.3:57850612-57850631 MS.gene55921:CDS 50.0%
! GCAGCTTGTGCAGTTGATTC+AGG + chr2.3:57850638-57850657 MS.gene55921:CDS 50.0%
! GGAGGTACAGGATCAGTAAC+AGG + chr2.3:57853290-57853309 MS.gene55921:CDS 50.0%
! GGTACTTTGATGGCACCAGA+TGG + chr2.3:57851147-57851166 MS.gene55921:CDS 50.0%
! TGACTGAGTTGCGGACTGTT+AGG - chr2.3:57853229-57853248 None:intergenic 50.0%
! TGGACCAAATTGTTGGCCTG+AGG + chr2.3:57851167-57851186 MS.gene55921:CDS 50.0%
! TGGCTTTTACTGTCTGCCTC+AGG - chr2.3:57851186-57851205 None:intergenic 50.0%
! TTCGACAGCATTGGAAGCCT+TGG + chr2.3:57853173-57853192 MS.gene55921:intron 50.0%
AGGTGAGCCTGAAACGGTTG+TGG + chr2.3:57853637-57853656 MS.gene55921:CDS 55.0%
GAAGGTGGTGAAGGGTCACT+AGG - chr2.3:57850458-57850477 None:intergenic 55.0%
GTGCTCCTACCCACGGAATT+AGG + chr2.3:57851930-57851949 MS.gene55921:CDS 55.0%
TGGTCCATGCAAACCAGGAC+TGG + chr2.3:57850715-57850734 MS.gene55921:CDS 55.0%
! CTGTTTTGTCCCCTGCTCCT+AGG + chr2.3:57852379-57852398 MS.gene55921:intron 55.0%
GGTGAAGGGTCACTAGGTGC+TGG - chr2.3:57850452-57850471 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.3 gene 57850386 57853688 57850386 ID=MS.gene55921
chr2.3 mRNA 57850386 57853688 57850386 ID=MS.gene55921.t1;Parent=MS.gene55921
chr2.3 exon 57850386 57850745 57850386 ID=MS.gene55921.t1.exon1;Parent=MS.gene55921.t1
chr2.3 CDS 57850386 57850745 57850386 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
chr2.3 exon 57851141 57851308 57851141 ID=MS.gene55921.t1.exon2;Parent=MS.gene55921.t1
chr2.3 CDS 57851141 57851308 57851141 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
chr2.3 exon 57851405 57851449 57851405 ID=MS.gene55921.t1.exon3;Parent=MS.gene55921.t1
chr2.3 CDS 57851405 57851449 57851405 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
chr2.3 exon 57851561 57851768 57851561 ID=MS.gene55921.t1.exon4;Parent=MS.gene55921.t1
chr2.3 CDS 57851561 57851768 57851561 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
chr2.3 exon 57851870 57851951 57851870 ID=MS.gene55921.t1.exon5;Parent=MS.gene55921.t1
chr2.3 CDS 57851870 57851951 57851870 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
chr2.3 exon 57853181 57853688 57853181 ID=MS.gene55921.t1.exon6;Parent=MS.gene55921.t1
chr2.3 CDS 57853181 57853688 57853181 ID=cds.MS.gene55921.t1;Parent=MS.gene55921.t1
Gene Sequence

>MS.gene55921

ATGATTCTTTTCATCCATAATGTGGAAGGAAGGTACCATCACAAGAAACCAAAGAAAACTTCTCCAGCACCTAGTGACCCTTCACCACCTTCTTCCAATCCTCCTACATCTCCTAGTGTTCCATCTGATCCATATCCATATCCAAATAATCCTGGAGAATCTCCTTCGGATTGCGTTTTCGATGTAAGATCATTTGGAGCAGTTGGAGATGGTAATGCTGATGATACAGCTGCATTTAGAGCAGCATGGAAAGCAGCTTGTGCAGTTGATTCAGGAGTTCTTTTAGCTCCTGAAAATTACTGTTTTAAAATCACTTCTACTATCTTTTCTGGTCCATGCAAACCAGGACTGGTATTTCAAGTAAGAATGTTTCTTAAATTTCCTTTTATGTTCACGGTTGCGTTGCGATTCTTTAAATTTTAGACAAATGTGGCTGATGTGGCTGCAATTATGGTTGCAAAGGCTGCAGAAACTTTTACGCTGTGGTCGCAATTGCTGTTATAGACTGCAATTTAGAATCACGATTGTGGTTTTAAATTGTGGTCTGCAACAGTAATAGAGGCCGCAGCGTAAAGGATTTTGAAGTCTCTTCAAAGTCATTGTAACTATAATTTTGACTGCATCAGCTGCATTTTCTCTAATTTATCCGCAATATAAAAGTTTGCAGCGTAATTGGAACTGAAATTTAACATGACCATCATTGTGTAAAATTTAATTTCCTATATAATTAACTAACAAGGTTGAAATATATGTAGATTGATGGTACTTTGATGGCACCAGATGGACCAAATTGTTGGCCTGAGGCAGACAGTAAAAGCCAATGGCTTGTGTTTTATAGACTTGATCAAATGACTCTAAATGGGACAGGAATTATTGAAGGCAATGGAGAACAATGGTGGGATCTTCCTTGCAAACCTCATAGGGTAATAATAATAATTATTATTAAAACTTTATTTTTTTTTATTTATTTATCTAATGTTAAAAACTAAAATTCTAGTAATTTTTCATTTTCATTTCAGAGTCCAAATGGAAAAACTGTATCAGGACCATGTGTTAGCCCTACAGTGAGTAAAATATTCAGAATTTACCTTAACCATTACATCACAGTTTCTTTTTTTTTTTTTAAATATGAAATGCCTTATAATTTTTTTTCTTATGAAATATCTTGTTTATAGATGATAAGGTTCTTCATGAGCTCAAATTTGGTGTTAAGAGGGTTGAAAATACAAAATAGTCCTCAATTCCATGTGAAATTTGATGGTTGTCAAGGAGTATTAATTGATGAATTGTCCATTTCTGCACCAAAACTTAGTCCCAATACTGATGGAATCCACTTAGGAAACACAAGGGATGTTGGAATATATAATTCCTTGATAAGCAATGGTAAATAATAAAGATCATTGTCACACTTTCTCAATATAGAATTTTTATTCTATGGTTTTTTTATTTAATTATGTCACATAATGTTACATTTTATTTAATAGGTGATGATTGCATTTCAATTGGTCCTGGATGTTCAGATGTGAATGTGGATGGTGTTACTTGTGCTCCTACCCACGGAATTAGGTATGTCACATAGTTTCTGTTATTTTAGTTAAATTGGGTTGTTAACTATCAATTAATTATGATTAATTTAGGAATAAAATAAAAATAAGCTACAAACTTAGTTGATGGTATTCATATAAATATATACACCCAAATTCTGTGGTCCAAAAATCATTTCAAAAGGCAAAATGAATGAAAAGTGACTTTAACCTGTAGTGTGAGTGTAATAACTCTTGAAGGTGTCTTATAAGATAGCAATTTATCTTCAAGAAAAATAAATAAATAATGGAAAATTTTTGTTTGAAATTGAAACCTTTTTAACATCAAATGATCACAGGGTTAATTTTCTTTTTTAGAGATTTATGTGACTATAGTACTTTAACAGTTGGAAGTTTCAAACTGCCAATCATTAATGTGACAATAGTTTTTGTTCTTCACTTCTGTAATTCTGTTTTGTCCCCTGCTCCTAGGTAGCAATCTATTACCTTGGGACTAATTTTTTGGACATGACTTTTAAAGATGTTGATTTTGTAAAACTTATTAACATAAAATTGAGTTTAAAGAAAACTGATTTATATTCATATGATTTTATTTTAAAATCACAGTTTATTATTTTAGAGGTATAAAACTGATTTTATATGTTTGGATGTTATAGAGTATAATTGATATTGTCTCAAAAATTAACTCTAACTTAAAGCTAGAATTTTGAGCTTCTAACTCTCCAATTGTTTTTAAGATTAAGTTTTTTTTTTCAATTCACTTTTACATAAATATATACAACAAAAAATAAATTTACATTCACGCACTACTTATACCACAGTTGATGAGAAGTACACACTTAGTGGACTAAACATGTTATAACATCTACGAAAATGTCTGATACTAGAGACCAAAATAAACCTGAAAAAAAAAAAAAAAAACTCTGAAAACATTTACAATCTTTCATAAAATAATTACATAATTGCAATCAGTGTCATAGATTAGAAGTTGTTTTATGTTGTATGGCTTGATTCCAATTATGTAATCATTTTATGAAAAATTGTAAATGTGCATTGAGTTATGAGAGTTATTTTTTTTCTTCAAATTTATTTTGGTCTCTAGTATACATCTTCTTAGAATTATTTTGGTCTCTAGTATGAGACATCTTCCTAGAAGTTACATGTGATGTGCTATTTTGGAACCTATAGAATTTTGAGTAGCAAGCTTCTATGTAGCTATAATTAAAAACCTTACTGATGTGATTTCGACAGCATTGGAAGCCTTGGAGTACACAATTCCCACGCATGTGTCTCAAACCTAACAGTCCGCAACTCAGTCATAAAAGAATCAGACAATGGACTAAGAATCAAAACATGGCAAGGAGGTACAGGATCAGTAACAGGTTTAACATTTGACAACATCCAAATGGAGAATGTAAGAAACTGTATCAACATAGACCAATTCTACTGTTTATCAAAAGAGTGTATGAACCAAACATCAGCAGTATATGTAAACAATATCTCCTACAGAAAAATAAAGGGTACATATGATGTGAGAACACCTCCAATACACTTTGCTTGTAGTGACACTGTTGCTTGCACAAACATCACACTCTCTGAGATTGAACTTTTACCTTATGAAGGTGAATTACTTGATGACCCTTTTTGTTGGAATGCTTATGGAAGACAAGAGACTTTGACTATTCCTCCACTTGATTGTTTAAGGGAAGGTGAGCCTGAAACGGTTGTGGAGTTATCTGAATATGAATGCAATAATTAA
Protein sequence

>MS.gene55921.t1

MILFIHNVEGRYHHKKPKKTSPAPSDPSPPSSNPPTSPSVPSDPYPYPNNPGESPSDCVFDVRSFGAVGDGNADDTAAFRAAWKAACAVDSGVLLAPENYCFKITSTIFSGPCKPGLVFQIDGTLMAPDGPNCWPEADSKSQWLVFYRLDQMTLNGTGIIEGNGEQWWDLPCKPHRSPNGKTVSGPCVSPTMIRFFMSSNLVLRGLKIQNSPQFHVKFDGCQGVLIDELSISAPKLSPNTDGIHLGNTRDVGIYNSLISNGDDCISIGPGCSDVNVDGVTCAPTHGISIGSLGVHNSHACVSNLTVRNSVIKESDNGLRIKTWQGGTGSVTGLTFDNIQMENVRNCINIDQFYCLSKECMNQTSAVYVNNISYRKIKGTYDVRTPPIHFACSDTVACTNITLSEIELLPYEGELLDDPFCWNAYGRQETLTIPPLDCLREGEPETVVELSEYECNN