AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene058304

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058304.t1 MTR_2g032710 96.272 456 8 2 1 453 14 463 0.0 900
MS.gene058304.t1 MTR_4g094715 73.304 457 108 3 1 453 15 461 0.0 690
MS.gene058304.t1 MTR_1g086390 62.821 390 140 2 59 443 80 469 0.0 538
MS.gene058304.t1 MTR_7g110050 60.000 425 157 5 32 451 46 462 0.0 529
MS.gene058304.t1 MTR_5g034090 45.952 457 204 10 18 441 77 523 6.41e-128 381
MS.gene058304.t1 MTR_8g065010 47.319 429 187 8 41 440 91 509 8.66e-126 375
MS.gene058304.t1 MTR_6g005630 45.736 387 189 7 60 442 45 414 9.91e-115 343
MS.gene058304.t1 MTR_5g033680 41.337 404 215 9 43 438 50 439 1.17e-103 317
MS.gene058304.t1 MTR_6g005630 42.636 387 178 7 60 442 45 391 1.16e-100 306
MS.gene058304.t1 MTR_8g065030 38.164 414 233 8 48 449 53 455 9.64e-92 286
MS.gene058304.t1 MTR_2g103410 39.842 379 212 8 60 432 59 427 2.28e-86 271
MS.gene058304.t1 MTR_1g052315 42.778 360 184 10 54 404 57 403 3.98e-85 268
MS.gene058304.t1 MTR_7g091850 41.341 358 194 6 59 410 75 422 2.18e-83 264
MS.gene058304.t1 MTR_5g034090 45.238 336 144 8 18 321 77 404 9.08e-83 261
MS.gene058304.t1 MTR_2g060350 39.118 363 203 8 54 409 68 419 1.52e-78 251
MS.gene058304.t1 MTR_7g091840 40.419 334 182 6 49 374 54 378 2.99e-77 246
MS.gene058304.t1 MTR_6g028080 36.567 402 215 10 54 435 16 397 8.81e-73 234
MS.gene058304.t1 MTR_2g103650 34.805 385 236 7 60 437 28 404 8.40e-72 232
MS.gene058304.t1 MTR_1g012390 36.504 389 225 9 56 437 24 397 5.20e-70 228
MS.gene058304.t1 MTR_3g054320 35.623 393 222 10 60 443 30 400 5.24e-68 222
MS.gene058304.t1 MTR_3g030540 38.292 363 197 9 61 414 71 415 1.48e-66 219
MS.gene058304.t1 MTR_3g084380 35.196 358 209 6 60 409 21 363 3.19e-65 215
MS.gene058304.t1 MTR_4g084050 34.795 365 208 8 60 410 23 371 8.03e-64 211
MS.gene058304.t1 MTR_2g049400 34.884 387 232 8 53 435 21 391 1.91e-63 210
MS.gene058304.t1 MTR_2g058670 34.943 352 214 8 59 404 24 366 5.58e-63 210
MS.gene058304.t1 MTR_3g084360 34.262 359 213 8 60 410 24 367 1.13e-62 208
MS.gene058304.t1 MTR_8g010040 35.770 383 211 9 60 434 32 387 1.21e-62 208
MS.gene058304.t1 MTR_1g088160 35.040 371 220 11 59 420 34 392 2.10e-62 208
MS.gene058304.t1 MTR_2g058640 35.977 353 209 9 59 404 24 366 4.30e-62 207
MS.gene058304.t1 MTR_8g010070 35.052 388 220 12 57 437 28 390 8.43e-62 206
MS.gene058304.t1 MTR_8g010080 35.509 383 215 11 59 435 30 386 1.81e-61 205
MS.gene058304.t1 MTR_5g043350 34.103 390 229 10 60 439 29 400 2.57e-61 205
MS.gene058304.t1 MTR_2g058840 34.262 359 205 9 59 404 24 364 4.04e-61 205
MS.gene058304.t1 MTR_3g438050 33.962 371 223 8 59 421 26 382 8.57e-61 203
MS.gene058304.t1 MTR_5g097860 37.730 326 183 8 59 376 24 337 4.85e-60 200
MS.gene058304.t1 MTR_4g083660 34.615 390 231 12 53 435 2 374 1.24e-59 199
MS.gene058304.t1 MTR_0157s0060 34.031 382 221 8 65 437 3 362 1.26e-59 199
MS.gene058304.t1 MTR_4g034020 34.031 382 221 8 65 437 3 362 1.26e-59 199
MS.gene058304.t1 MTR_7g113910 35.792 366 151 8 59 422 56 339 2.62e-59 199
MS.gene058304.t1 MTR_8g008765 35.474 327 183 6 56 376 27 331 2.96e-59 198
MS.gene058304.t1 MTR_7g035415 36.508 315 173 7 59 365 39 334 4.88e-59 197
MS.gene058304.t1 MTR_8g010110 32.987 385 227 9 57 435 28 387 2.84e-58 196
MS.gene058304.t1 MTR_8g010090 33.247 385 226 10 57 435 28 387 4.14e-57 193
MS.gene058304.t1 MTR_2g435640 34.262 359 217 7 54 404 47 394 6.36e-57 194
MS.gene058304.t1 MTR_2g103410 37.453 267 154 5 60 321 59 317 1.01e-55 188
MS.gene058304.t1 MTR_8g009810 35.196 358 210 10 60 410 35 377 5.15e-55 188
MS.gene058304.t1 MTR_1g013730 32.984 382 222 9 60 435 27 380 1.78e-54 186
MS.gene058304.t1 MTR_8g030930 33.705 359 213 10 59 406 26 370 4.15e-54 186
MS.gene058304.t1 MTR_1g013740 32.552 384 225 9 60 437 35 390 1.40e-53 184
MS.gene058304.t1 MTR_2g029170 35.000 340 199 10 102 435 38 361 6.63e-53 181
MS.gene058304.t1 MTR_4g120320 32.727 385 241 9 55 432 47 420 1.44e-52 182
MS.gene058304.t1 MTR_8g010050 31.088 386 200 12 57 435 28 354 4.47e-50 174
MS.gene058304.t1 MTR_8g077320 30.000 390 201 8 59 434 32 363 1.19e-47 168
MS.gene058304.t1 MTR_8g008700 33.750 320 165 8 118 435 16 290 3.25e-46 162
MS.gene058304.t1 MTR_8g031180 36.538 208 122 3 204 405 70 273 5.76e-39 142
MS.gene058304.t1 MTR_5g063430 30.844 308 184 9 59 356 24 312 9.09e-39 142
MS.gene058304.t1 MTR_2g100040 30.854 363 180 14 59 412 24 324 4.63e-36 137
MS.gene058304.t1 MTR_4g124980 26.077 418 242 14 48 423 62 454 1.92e-21 97.1
MS.gene058304.t1 MTR_2g042740 25.916 382 231 13 58 404 54 418 3.89e-20 93.2
MS.gene058304.t1 MTR_3g449590 24.574 411 263 12 62 440 49 444 9.11e-18 85.9
MS.gene058304.t1 MTR_5g090160 26.198 313 187 10 65 346 49 348 1.67e-16 82.0
MS.gene058304.t1 MTR_2g435340 26.364 330 193 12 50 345 27 340 1.14e-14 76.3
MS.gene058304.t1 MTR_8g098840 24.928 349 207 14 62 373 46 376 1.62e-14 75.9
MS.gene058304.t1 MTR_1g100040 25.962 312 179 13 65 341 61 355 1.08e-13 73.2
MS.gene058304.t1 MTR_1g100050 29.614 233 125 9 65 273 55 272 1.59e-13 72.8
MS.gene058304.t1 MTR_7g088550 25.566 309 182 12 65 340 55 348 1.71e-13 72.8
MS.gene058304.t1 MTR_7g110710 26.852 324 185 11 62 350 45 351 1.93e-13 72.4
MS.gene058304.t1 MTR_7g110710 26.852 324 185 11 62 350 45 351 2.86e-13 72.0
MS.gene058304.t1 MTR_1g099850 25.161 310 184 12 65 341 60 354 4.83e-13 71.2
MS.gene058304.t1 MTR_3g096330 24.611 321 192 10 55 341 71 375 5.74e-13 71.6
MS.gene058304.t1 MTR_1g086030 26.006 323 188 11 62 350 84 389 2.49e-12 69.3
MS.gene058304.t1 MTR_1g100060 27.468 233 130 8 65 273 98 315 3.01e-12 68.9
MS.gene058304.t1 MTR_8g028725 23.392 342 221 10 62 373 57 387 3.47e-12 68.6
MS.gene058304.t1 MTR_2g435340 27.230 213 132 6 145 345 74 275 9.34e-12 67.0
MS.gene058304.t1 MTR_7g078680 23.058 412 268 11 65 443 60 455 2.21e-11 66.2
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene058304.t1 AT1G48100 70.601 449 122 4 4 445 21 466 0.0 647
MS.gene058304.t1 AT1G02460 61.637 391 142 4 59 441 88 478 1.05e-178 509
MS.gene058304.t1 AT4G01890 55.779 398 168 3 48 437 53 450 2.40e-166 477
MS.gene058304.t1 AT1G56710 54.126 412 178 5 39 441 24 433 1.84e-164 471
MS.gene058304.t1 AT1G60590 44.769 411 191 7 60 440 123 527 1.09e-116 352
MS.gene058304.t1 AT1G10640 44.743 409 193 7 59 440 118 520 2.16e-115 349
MS.gene058304.t1 AT5G14650 44.938 405 204 7 53 451 44 435 2.21e-113 340
MS.gene058304.t1 AT3G26610 40.724 442 232 9 7 437 27 449 6.12e-104 317
MS.gene058304.t1 AT1G70500 40.909 396 218 7 59 449 66 450 1.91e-96 298
MS.gene058304.t1 AT1G80170 41.361 382 209 7 56 431 49 421 2.16e-96 297
MS.gene058304.t1 AT1G23460 41.667 384 204 8 59 435 42 412 8.45e-93 288
MS.gene058304.t1 AT1G23460 41.667 384 204 8 59 435 66 436 1.29e-92 288
MS.gene058304.t1 AT3G57510 41.143 350 188 7 62 404 70 408 7.83e-83 262
MS.gene058304.t1 AT2G41850 40.857 350 189 7 62 404 70 408 1.43e-82 261
MS.gene058304.t1 AT3G07970 38.992 377 209 9 51 420 64 426 5.98e-80 254
MS.gene058304.t1 AT1G80140 35.714 350 204 6 92 435 2 336 3.12e-69 223
MS.gene058304.t1 AT3G15720 37.468 387 212 11 60 436 24 390 8.82e-69 226
MS.gene058304.t1 AT3G15720 37.468 387 212 11 60 436 23 389 9.07e-69 226
MS.gene058304.t1 AT4G35670 34.551 356 212 6 60 409 24 364 3.44e-68 223
MS.gene058304.t1 AT5G14650 42.491 273 142 4 53 321 44 305 3.99e-68 220
MS.gene058304.t1 AT3G15720 37.468 387 209 12 60 436 23 386 4.22e-66 219
MS.gene058304.t1 AT2G43870 37.607 351 199 7 60 404 21 357 6.06e-64 211
MS.gene058304.t1 AT5G44840 33.067 375 222 9 59 423 23 378 1.55e-63 210
MS.gene058304.t1 AT2G43880 35.450 378 219 10 60 430 31 390 2.51e-63 210
MS.gene058304.t1 AT1G80170 40.221 271 149 5 56 321 49 311 1.36e-62 206
MS.gene058304.t1 AT1G65570 36.735 392 223 12 52 435 23 397 3.20e-62 207
MS.gene058304.t1 AT1G78400 35.156 384 230 7 59 436 34 404 1.36e-61 206
MS.gene058304.t1 AT1G05660 33.333 384 235 9 59 437 27 394 2.36e-61 205
MS.gene058304.t1 AT5G27530 33.889 360 205 7 59 410 52 386 1.75e-60 204
MS.gene058304.t1 AT2G43890 35.106 376 223 12 60 430 29 388 2.27e-60 202
MS.gene058304.t1 AT1G05650 33.077 390 240 9 53 437 21 394 3.39e-60 202
MS.gene058304.t1 AT3G59850 36.544 353 201 10 60 404 23 360 3.90e-60 201
MS.gene058304.t1 AT3G59850 36.544 353 201 10 60 404 67 404 1.21e-59 201
MS.gene058304.t1 AT1G02790 36.158 354 196 10 59 404 51 382 4.01e-59 200
MS.gene058304.t1 AT1G17150 35.789 380 224 9 59 431 32 398 8.31e-59 198
MS.gene058304.t1 AT2G43860 35.844 385 223 12 60 437 33 400 8.41e-59 198
MS.gene058304.t1 AT2G33160 35.904 376 217 10 40 404 11 373 1.65e-58 204
MS.gene058304.t1 AT5G48140 35.588 340 195 10 73 404 34 357 6.32e-58 196
MS.gene058304.t1 AT3G07830 36.686 338 195 8 73 404 34 358 8.20e-58 196
MS.gene058304.t1 AT3G07840 35.613 351 206 9 65 409 26 362 1.42e-57 195
MS.gene058304.t1 AT3G15720 36.628 344 189 10 102 436 3 326 3.17e-56 191
MS.gene058304.t1 AT4G13760 33.989 356 212 10 59 404 30 372 4.53e-55 189
MS.gene058304.t1 AT3G07820 35.860 343 200 9 73 409 33 361 4.72e-55 188
MS.gene058304.t1 AT5G17200 31.785 409 230 10 56 437 35 421 6.84e-55 189
MS.gene058304.t1 AT1G43080 34.174 357 210 11 59 404 30 372 2.93e-54 186
MS.gene058304.t1 AT5G39910 30.402 398 194 11 59 436 35 369 7.85e-54 184
MS.gene058304.t1 AT5G44830 31.765 340 199 7 90 423 7 319 9.37e-54 183
MS.gene058304.t1 AT1G43090 34.270 356 211 10 59 404 30 372 9.73e-54 186
MS.gene058304.t1 AT4G32380 32.432 370 222 7 72 430 39 391 1.21e-53 186
MS.gene058304.t1 AT3G14040 37.396 361 205 9 52 405 63 409 1.51e-53 186
MS.gene058304.t1 AT4G18180 35.294 357 207 9 59 404 38 381 2.19e-53 184
MS.gene058304.t1 AT2G43880 36.824 296 170 6 60 351 31 313 1.16e-52 180
MS.gene058304.t1 AT1G43100 34.174 357 210 11 59 404 30 372 2.97e-52 182
MS.gene058304.t1 AT4G32375 36.655 281 151 6 131 404 5 265 1.70e-51 179
MS.gene058304.t1 AT2G15460 34.078 358 209 12 59 404 30 372 1.76e-51 179
MS.gene058304.t1 AT2G26620 33.616 354 214 10 59 404 30 370 2.48e-51 179
MS.gene058304.t1 AT2G15450 33.799 358 210 12 59 404 30 372 5.02e-51 178
MS.gene058304.t1 AT3G07850 37.119 361 205 10 52 405 63 408 5.63e-51 179
MS.gene058304.t1 AT2G40310 33.333 357 211 11 60 404 31 372 1.37e-50 177
MS.gene058304.t1 AT2G15470 33.520 358 211 12 59 404 30 372 1.52e-49 174
MS.gene058304.t1 AT4G35670 32.841 271 168 3 139 409 1 257 5.90e-49 169
MS.gene058304.t1 AT4G32370 35.495 293 164 6 74 355 49 327 1.95e-47 167
MS.gene058304.t1 AT4G32380 35.316 269 150 5 72 332 39 291 2.96e-44 160
MS.gene058304.t1 AT5G41870 27.586 319 187 9 59 346 44 349 5.42e-22 98.6
MS.gene058304.t1 AT3G42950 27.869 427 255 16 58 448 64 473 2.01e-21 97.1
MS.gene058304.t1 AT1G19170 25.722 381 234 12 58 404 85 450 7.07e-20 92.4
MS.gene058304.t1 AT4G33440 27.673 318 186 11 55 341 67 371 7.11e-18 86.3
MS.gene058304.t1 AT3G62110 26.250 320 192 10 62 350 45 351 4.81e-17 83.6
MS.gene058304.t1 AT3G62110 26.250 320 192 10 62 350 45 351 4.81e-17 83.6
MS.gene058304.t1 AT4G23820 27.586 319 180 13 62 346 43 344 2.09e-16 81.6
MS.gene058304.t1 AT2G23900 24.173 393 237 16 65 419 61 430 4.88e-14 74.3
MS.gene058304.t1 AT3G48950 23.121 346 212 12 65 373 50 378 6.07e-14 73.9
MS.gene058304.t1 AT4G23500 24.515 412 264 12 65 444 73 469 2.48e-12 69.3

Find 107 sgRNAs with CRISPR-Local

Find 191 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
TTCCTCCACTTGATTGTTTA+AGG 0.078821 2.2:+56023911 MS.gene058304:CDS
CTTGATCAAATGACTCTAAA+TGG 0.174861 2.2:+56021492 MS.gene058304:CDS
GGAGATTCTCCAGGATTATT+TGG 0.184472 2.2:-56020794 None:intergenic
GAATATGAATGCAATAATTA+AGG 0.201144 2.2:+56023967 MS.gene058304:CDS
GGTACCTTCCTTCCACATTA+TGG 0.224070 2.2:-56020667 None:intergenic
GATAACTCCACAACCGTTTC+AGG 0.228355 2.2:-56023943 None:intergenic
GTTCTTCATGAGCTCAAATT+TGG 0.241900 2.2:+56021841 MS.gene058304:CDS
CCATAAGCATTCCAACAAAA+AGG 0.243640 2.2:-56023868 None:intergenic
TCTCCAGGATTATTTGGATA+TGG 0.275950 2.2:-56020788 None:intergenic
GCAGCTTGTGCAGTTGATTC+AGG 0.281433 2.2:+56020905 MS.gene058304:CDS
GGATTATTTGGATATGGATA+TGG 0.286489 2.2:-56020782 None:intergenic
GTGCTCCTACCCACGGAATT+AGG 0.301522 2.2:+56022202 MS.gene058304:CDS
CAATTCCATGTGAAATTTGA+TGG 0.330222 2.2:+56021896 MS.gene058304:CDS
TCTGCCTCAGGCCAACAATT+TGG 0.335193 2.2:-56021438 None:intergenic
AATCTTGTTTATAGATGATA+AGG 0.354998 2.2:+56021819 MS.gene058304:intron
ATCTTCCTTGCAAACCTCAT+AGG 0.364129 2.2:+56021553 MS.gene058304:CDS
AGTTTCTTACATTCTCCATT+TGG 0.366739 2.2:-56023630 None:intergenic
TCCTCCACTTGATTGTTTAA+GGG 0.382641 2.2:+56023912 MS.gene058304:CDS
TTCGACAGCATTGGAAGCCT+TGG 0.386798 2.2:+56023472 MS.gene058304:intron
GGCCAACAATTTGGTCCATC+TGG 0.390506 2.2:-56021429 None:intergenic
AGCTCAAATTTGGTGTTAAG+AGG 0.391670 2.2:+56021851 MS.gene058304:CDS
TGACTGAGTTGCGGACTGTT+AGG 0.400859 2.2:-56023525 None:intergenic
ATTATTATTATTACCCTATG+AGG 0.401164 2.2:-56021567 None:intergenic
TCAGTATTGGGACTAAGTTT+TGG 0.402102 2.2:-56021959 None:intergenic
GGTGATGATTGCATTTCAAT+TGG 0.404860 2.2:+56022141 MS.gene058304:intron
TTATTTACCATTGCTTATCA+AGG 0.419053 2.2:-56022026 None:intergenic
AAGTGGATTCCATCAGTATT+GGG 0.420148 2.2:-56021971 None:intergenic
AATATGAATGCAATAATTAA+GGG 0.424968 2.2:+56023968 MS.gene058304:CDS
CATCCCTTGTGTTTCCTAAG+TGG 0.427078 2.2:-56021988 None:intergenic
TTGAAATATATGTAGATTGA+TGG 0.430307 2.2:+56021393 MS.gene058304:intron
TTACATACACTGCTGATGTT+TGG 0.440754 2.2:-56023699 None:intergenic
GGAGGTACAGGATCAGTAAC+AGG 0.441303 2.2:+56023589 MS.gene058304:CDS
TAAGTGGATTCCATCAGTAT+TGG 0.443227 2.2:-56021972 None:intergenic
ACTAGGAGATGTAGGAGGAT+TGG 0.444391 2.2:-56020747 None:intergenic
AACATTTGACAACATCCAAA+TGG 0.452717 2.2:+56023615 MS.gene058304:CDS
TCAAATTTCACATGGAATTG+AGG 0.453281 2.2:-56021893 None:intergenic
TTGATCAAATGACTCTAAAT+GGG 0.457341 2.2:+56021493 MS.gene058304:CDS
GTAGATTGATGGTACTTTGA+TGG 0.462628 2.2:+56021404 MS.gene058304:intron
GATTGCATTTCAATTGGTCC+TGG 0.464171 2.2:+56022147 MS.gene058304:CDS
GTAAGATCATTTGGAGCAGT+TGG 0.464835 2.2:+56020836 MS.gene058304:CDS
CACCAGATGGACCAAATTGT+TGG 0.467082 2.2:+56021427 MS.gene058304:CDS
CTTACTTGAAATACCAGTCC+TGG 0.467508 2.2:-56020995 None:intergenic
GGATTGGAAGAAGGTGGTGA+AGG 0.469757 2.2:-56020731 None:intergenic
AAAACATGGCAAGGAGGTAC+AGG 0.486274 2.2:+56023577 MS.gene058304:CDS
TCACTACAAGCAAAGTGTAT+TGG 0.494006 2.2:-56023772 None:intergenic
TATCCATATCCAAATAATCC+TGG 0.495114 2.2:+56020785 MS.gene058304:CDS
GGATATGGATATGGATCAGA+TGG 0.495462 2.2:-56020773 None:intergenic
TCAACTAATTCACCTTCATA+AGG 0.499997 2.2:-56023841 None:intergenic
AGGTGAGCCTGAAACGGTTG+TGG 0.500843 2.2:+56023936 MS.gene058304:CDS
TGGTCCATGCAAACCAGGAC+TGG 0.506011 2.2:+56020982 MS.gene058304:CDS
AATACCAGTCCTGGTTTGCA+TGG 0.514150 2.2:-56020986 None:intergenic
GATGTAGGAGGATTGGAAGA+AGG 0.516798 2.2:-56020740 None:intergenic
TCTTCCTTGCAAACCTCATA+GGG 0.518124 2.2:+56021554 MS.gene058304:CDS
GGTGAAGGGTCACTAGGTGC+TGG 0.521610 2.2:-56020716 None:intergenic
TAATCCTGGAGAATCTCCTT+CGG 0.522281 2.2:+56020799 MS.gene058304:CDS
AAACTTAGTCCCAATACTGA+TGG 0.523177 2.2:+56021962 MS.gene058304:CDS
AATACTGATGGAATCCACTT+AGG 0.526638 2.2:+56021974 MS.gene058304:CDS
CAAATGACTCTAAATGGGAC+AGG 0.527686 2.2:+56021498 MS.gene058304:CDS
CCACTTGATTGTTTAAGGGA+AGG 0.528218 2.2:+56023916 MS.gene058304:CDS
GATGGAACACTAGGAGATGT+AGG 0.530462 2.2:-56020755 None:intergenic
AATGGGACAGGAATTATTGA+AGG 0.532465 2.2:+56021510 MS.gene058304:CDS
GATTGGAAGAAGGTGGTGAA+GGG 0.533569 2.2:-56020730 None:intergenic
AAGGGAAGGTGAGCCTGAAA+CGG 0.547901 2.2:+56023930 MS.gene058304:CDS
GTCTATAAAACACAAGCCAT+TGG 0.548487 2.2:-56021470 None:intergenic
TCATTTGGAGCAGTTGGAGA+TGG 0.548866 2.2:+56020842 MS.gene058304:CDS
CTACAAGCAAAGTGTATTGG+AGG 0.549583 2.2:-56023769 None:intergenic
ACAGGAATTATTGAAGGCAA+TGG 0.554020 2.2:+56021516 MS.gene058304:CDS
GAAGGTGGTGAAGGGTCACT+AGG 0.554808 2.2:-56020722 None:intergenic
GCTCAAATTTGGTGTTAAGA+GGG 0.555873 2.2:+56021852 MS.gene058304:CDS
GTAGGAGGATTGGAAGAAGG+TGG 0.559194 2.2:-56020737 None:intergenic
TTAGGAAACACAAGGGATGT+TGG 0.561840 2.2:+56021992 MS.gene058304:CDS
TATAATTCCTTGATAAGCAA+TGG 0.563522 2.2:+56022019 MS.gene058304:CDS
GTGAAATTTGATGGTTGTCA+AGG 0.563909 2.2:+56021905 MS.gene058304:CDS
ATGTGACATACCTAATTCCG+TGG 0.569578 2.2:-56022212 None:intergenic
CAATCCGAAGGAGATTCTCC+AGG 0.577573 2.2:-56020803 None:intergenic
ACATCGAAAACGCAATCCGA+AGG 0.582946 2.2:-56020815 None:intergenic
TATGGATCAGATGGAACACT+AGG 0.584081 2.2:-56020764 None:intergenic
TCATCCATAATGTGGAAGGA+AGG 0.585473 2.2:+56020663 MS.gene058304:CDS
GTTACTTGTGCTCCTACCCA+CGG 0.586560 2.2:+56022195 MS.gene058304:CDS
AGGACCATGTGTTAGCCCTA+CGG 0.588017 2.2:+56021695 MS.gene058304:CDS
ATTACCCTATGAGGTTTGCA+AGG 0.592983 2.2:-56021558 None:intergenic
CTAAGAATCAAAACATGGCA+AGG 0.597482 2.2:+56023568 MS.gene058304:CDS
AAGGCAATGGAGAACAATGG+TGG 0.608620 2.2:+56021529 MS.gene058304:CDS
ACATTCACATCTGAACATCC+AGG 0.609157 2.2:-56022165 None:intergenic
CGTGGGAATTGTGTACTCCA+AGG 0.610752 2.2:-56023489 None:intergenic
TGTTCAGATGTGAATGTGGA+TGG 0.618209 2.2:+56022171 MS.gene058304:CDS
GTCATAAAAGAATCAGACAA+TGG 0.619796 2.2:+56023544 MS.gene058304:CDS
TCCCTTAAACAATCAAGTGG+AGG 0.624213 2.2:-56023913 None:intergenic
AGGCAATGGAGAACAATGGT+GGG 0.624461 2.2:+56021530 MS.gene058304:CDS
GACAACCATCAAATTTCACA+TGG 0.633858 2.2:-56021901 None:intergenic
AGGCAGACAGTAAAAGCCAA+TGG 0.642020 2.2:+56021454 MS.gene058304:CDS
CCTTCCCTTAAACAATCAAG+TGG 0.643008 2.2:-56023916 None:intergenic
GGTACTTTGATGGCACCAGA+TGG 0.645534 2.2:+56021414 MS.gene058304:CDS
TGGACCAAATTGTTGGCCTG+AGG 0.650727 2.2:+56021434 MS.gene058304:CDS
ACATACCTAATTCCGTGGGT+AGG 0.654879 2.2:-56022207 None:intergenic
GAATCCACTTAGGAAACACA+AGG 0.655820 2.2:+56021984 MS.gene058304:CDS
TGGATGTTCAGATGTGAATG+TGG 0.656226 2.2:+56022167 MS.gene058304:CDS
TAGGTTTGAGACACATGCGT+GGG 0.660709 2.2:-56023506 None:intergenic
TTGAAGGCAATGGAGAACAA+TGG 0.660810 2.2:+56021526 MS.gene058304:CDS
TTAGGTTTGAGACACATGCG+TGG 0.673355 2.2:-56023507 None:intergenic
CAGCTGCATTTAGAGCAGCA+TGG 0.685070 2.2:+56020879 MS.gene058304:CDS
TGTGACATACCTAATTCCGT+GGG 0.685322 2.2:-56022211 None:intergenic
GGAACACTAGGAGATGTAGG+AGG 0.693701 2.2:-56020752 None:intergenic
ATGGACTAAGAATCAAAACA+TGG 0.703229 2.2:+56023563 MS.gene058304:CDS
AGAATCAAAACATGGCAAGG+AGG 0.705826 2.2:+56023571 MS.gene058304:CDS
CTCACCGTAGGGCTAACACA+TGG 0.709479 2.2:-56021699 None:intergenic
AATCCACTTAGGAAACACAA+GGG 0.771508 2.2:+56021985 MS.gene058304:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!! ATAAATAAATAAATAAATAA+TGG + chr2.2:56022477-56022496 MS.gene058304:intron 0.0%
!! CAATTAATTATGATTAATTT+AGG + chr2.2:56022273-56022292 MS.gene058304:intron 10.0%
!!! AATATAGAATTTTTATTCTA+TGG + chr2.2:56022073-56022092 MS.gene058304:intron 10.0%
!!! AATGTTACATTTTATTTAAT+AGG + chr2.2:56022120-56022139 MS.gene058304:intron 10.0%
!!! TTTTTTCTTCAAATTTATTT+TGG + chr2.2:56023301-56023320 MS.gene058304:intron 10.0%
!! AACTTAATCTTAAAAACAAT+TGG - chr2.2:56022956-56022975 None:intergenic 15.0%
!! TCATAAAATGATTACATAAT+TGG - chr2.2:56023245-56023264 None:intergenic 15.0%
!!! AATTAAATTTTACACAATGA+TGG - chr2.2:56021350-56021369 None:intergenic 15.0%
!!! ATCAAAGTTTATTATTTTAG+AGG + chr2.2:56022831-56022850 MS.gene058304:intron 15.0%
!!! ATTTTAAAACAGTAATTTTC+AGG - chr2.2:56020944-56020963 None:intergenic 15.0%
!!! TAAAACTGATTTTATATGTT+TGG + chr2.2:56022856-56022875 MS.gene058304:intron 15.0%
!!! TTCTGTTATTTTAGTTAAAT+TGG + chr2.2:56022238-56022257 MS.gene058304:intron 15.0%
!!! TTTTCTTTTCAAGTTTATTT+TGG - chr2.2:56023124-56023143 None:intergenic 15.0%
!! ACCTTGTTAGTTAATTATAT+AGG - chr2.2:56021374-56021393 None:intergenic 20.0%
!! ATTATTATTATTACCCTATG+AGG - chr2.2:56021570-56021589 None:intergenic 20.0%
!! TCCTATATAATTAACTAACA+AGG + chr2.2:56021370-56021389 MS.gene058304:intron 20.0%
!! TTGAAATATATGTAGATTGA+TGG + chr2.2:56021393-56021412 MS.gene058304:intron 20.0%
!!! AATCTTGTTTATAGATGATA+AGG + chr2.2:56021819-56021838 MS.gene058304:intron 20.0%
!!! ACATCTTCTTAGAATTATTT+TGG + chr2.2:56023334-56023353 MS.gene058304:intron 20.0%
!!! CTTTAAATTTTAGACAAATG+TGG + chr2.2:56021063-56021082 MS.gene058304:intron 20.0%
!!! TAAATTTCCTTTTATGTTCA+CGG + chr2.2:56021027-56021046 MS.gene058304:intron 20.0%
! AAAAAGAAACTGTGATGTAA+TGG - chr2.2:56021749-56021768 None:intergenic 25.0%
! ACTATTGTCACATTAATGAT+TGG - chr2.2:56022637-56022656 None:intergenic 25.0%
! ATCTCCTACAGAAAAATAAA+GGG + chr2.2:56023727-56023746 MS.gene058304:CDS 25.0%
! TATAATTCCTTGATAAGCAA+TGG + chr2.2:56022019-56022038 MS.gene058304:CDS 25.0%
! TATCTCCTACAGAAAAATAA+AGG + chr2.2:56023726-56023745 MS.gene058304:CDS 25.0%
! TTATTTACCATTGCTTATCA+AGG - chr2.2:56022029-56022048 None:intergenic 25.0%
! TTGATCAAATGACTCTAAAT+GGG + chr2.2:56021493-56021512 MS.gene058304:CDS 25.0%
!! ATTGAACTTTTACCTTATGA+AGG + chr2.2:56023829-56023848 MS.gene058304:CDS 25.0%
!! CTTTTGATAAACAGTAGAAT+TGG - chr2.2:56023669-56023688 None:intergenic 25.0%
!!! AGAAGTTGTTTTATGTTGTA+TGG + chr2.2:56023212-56023231 MS.gene058304:intron 25.0%
!!! TTGCCTTTTGAAATGATTTT+TGG - chr2.2:56022370-56022389 None:intergenic 25.0%
AAACTGTGATGTAATGGTTA+AGG - chr2.2:56021743-56021762 None:intergenic 30.0%
AACATTTGACAACATCCAAA+TGG + chr2.2:56023615-56023634 MS.gene058304:CDS 30.0%
AGTTTCTTACATTCTCCATT+TGG - chr2.2:56023633-56023652 None:intergenic 30.0%
ATAAAAGTTTGCAGCGTAAT+TGG + chr2.2:56021306-56021325 MS.gene058304:intron 30.0%
ATATATACACCCAAATTCTG+TGG + chr2.2:56022343-56022362 MS.gene058304:intron 30.0%
CAATTCCATGTGAAATTTGA+TGG + chr2.2:56021896-56021915 MS.gene058304:CDS 30.0%
CATAAGCATTCCAACAAAAA+GGG - chr2.2:56023870-56023889 None:intergenic 30.0%
CTTGATCAAATGACTCTAAA+TGG + chr2.2:56021492-56021511 MS.gene058304:CDS 30.0%
CTTGCTACTCAAAATTCTAT+AGG - chr2.2:56023413-56023432 None:intergenic 30.0%
GACTATAGTACTTTAACAGT+TGG + chr2.2:56022598-56022617 MS.gene058304:intron 30.0%
GGATTATTTGGATATGGATA+TGG - chr2.2:56020785-56020804 None:intergenic 30.0%
GGTCCAAAAATCATTTCAAA+AGG + chr2.2:56022364-56022383 MS.gene058304:intron 30.0%
GTCATAAAAGAATCAGACAA+TGG + chr2.2:56023544-56023563 MS.gene058304:CDS 30.0%
TAAGCTACAAACTTAGTTGA+TGG + chr2.2:56022309-56022328 MS.gene058304:intron 30.0%
TATCCATATCCAAATAATCC+TGG + chr2.2:56020785-56020804 MS.gene058304:CDS 30.0%
TCAAATTTCACATGGAATTG+AGG - chr2.2:56021896-56021915 None:intergenic 30.0%
TCAACTAATTCACCTTCATA+AGG - chr2.2:56023844-56023863 None:intergenic 30.0%
TTCTAACTTCAAATGATCAC+AGG + chr2.2:56022546-56022565 MS.gene058304:intron 30.0%
! ATCACTTCTACTATCTTTTC+TGG + chr2.2:56020962-56020981 MS.gene058304:CDS 30.0%
! ATGGACTAAGAATCAAAACA+TGG + chr2.2:56023563-56023582 MS.gene058304:CDS 30.0%
! ATTCTTTTATGACTGAGTTG+CGG - chr2.2:56023537-56023556 None:intergenic 30.0%
! GATTCTTTTCATCCATAATG+TGG + chr2.2:56020655-56020674 MS.gene058304:CDS 30.0%
! GTGATCATTTGAAGTTAGAA+AGG - chr2.2:56022546-56022565 None:intergenic 30.0%
! GTTTTCGATGTAAGATCATT+TGG + chr2.2:56020827-56020846 MS.gene058304:CDS 30.0%
! TGAATATTTTACTCACCGTA+GGG - chr2.2:56021713-56021732 None:intergenic 30.0%
! TTTTCATTTCAGAGTCCAAA+TGG + chr2.2:56021660-56021679 MS.gene058304:intron 30.0%
!! ATTTTTGGACCACAGAATTT+GGG - chr2.2:56022355-56022374 None:intergenic 30.0%
!! TTTTCTTTGGTTTCTTGTGA+TGG - chr2.2:56020691-56020710 None:intergenic 30.0%
!!! ACGATTGTGGTTTTAAATTG+TGG + chr2.2:56021173-56021192 MS.gene058304:intron 30.0%
!!! TGTACCCTTTATTTTTCTGT+AGG - chr2.2:56023734-56023753 None:intergenic 30.0%
!!! TTACATGTGATGTGCTATTT+TGG + chr2.2:56023385-56023404 MS.gene058304:intron 30.0%
AAACTTAGTCCCAATACTGA+TGG + chr2.2:56021962-56021981 MS.gene058304:CDS 35.0%
AAGGTAATAGATTGCTACCT+AGG - chr2.2:56022700-56022719 None:intergenic 35.0%
AATACTGATGGAATCCACTT+AGG + chr2.2:56021974-56021993 MS.gene058304:CDS 35.0%
AATCCACTTAGGAAACACAA+GGG + chr2.2:56021985-56022004 MS.gene058304:CDS 35.0%
AATGGGACAGGAATTATTGA+AGG + chr2.2:56021510-56021529 MS.gene058304:CDS 35.0%
ACAGGAATTATTGAAGGCAA+TGG + chr2.2:56021516-56021535 MS.gene058304:CDS 35.0%
AGGTAGCAATCTATTACCTT+GGG + chr2.2:56022700-56022719 MS.gene058304:intron 35.0%
AGTTATTACACTCACACTAC+AGG - chr2.2:56022415-56022434 None:intergenic 35.0%
CCAAATGGAAAAACTGTATC+AGG + chr2.2:56021675-56021694 MS.gene058304:CDS 35.0%
CCATAAGCATTCCAACAAAA+AGG - chr2.2:56023871-56023890 None:intergenic 35.0%
CTAAGAATCAAAACATGGCA+AGG + chr2.2:56023568-56023587 MS.gene058304:CDS 35.0%
GACAACCATCAAATTTCACA+TGG - chr2.2:56021904-56021923 None:intergenic 35.0%
GGTGATGATTGCATTTCAAT+TGG + chr2.2:56022141-56022160 MS.gene058304:intron 35.0%
GTCTATAAAACACAAGCCAT+TGG - chr2.2:56021473-56021492 None:intergenic 35.0%
GTGAGTGTAATAACTCTTGA+AGG + chr2.2:56022420-56022439 MS.gene058304:intron 35.0%
GTTCTTCATGAGCTCAAATT+TGG + chr2.2:56021841-56021860 MS.gene058304:CDS 35.0%
TAGGTAGCAATCTATTACCT+TGG + chr2.2:56022699-56022718 MS.gene058304:intron 35.0%
TCACTACAAGCAAAGTGTAT+TGG - chr2.2:56023775-56023794 None:intergenic 35.0%
TCCTCCACTTGATTGTTTAA+GGG + chr2.2:56023912-56023931 MS.gene058304:CDS 35.0%
TCTCCAGGATTATTTGGATA+TGG - chr2.2:56020791-56020810 None:intergenic 35.0%
TTACATACACTGCTGATGTT+TGG - chr2.2:56023702-56023721 None:intergenic 35.0%
TTCCTCCACTTGATTGTTTA+AGG + chr2.2:56023911-56023930 MS.gene058304:CDS 35.0%
! AAGTGGATTCCATCAGTATT+GGG - chr2.2:56021974-56021993 None:intergenic 35.0%
! CCTGATACAGTTTTTCCATT+TGG - chr2.2:56021678-56021697 None:intergenic 35.0%
! CCTTTTTGTTGGAATGCTTA+TGG + chr2.2:56023868-56023887 MS.gene058304:CDS 35.0%
! CGCTGCAAACTTTTATATTG+CGG - chr2.2:56021302-56021321 None:intergenic 35.0%
! CTGAATATTTTACTCACCGT+AGG - chr2.2:56021714-56021733 None:intergenic 35.0%
! CTTTTCATCCATAATGTGGA+AGG + chr2.2:56020659-56020678 MS.gene058304:CDS 35.0%
! GCAATTTAGAATCACGATTG+TGG + chr2.2:56021160-56021179 MS.gene058304:intron 35.0%
! GTAGATTGATGGTACTTTGA+TGG + chr2.2:56021404-56021423 MS.gene058304:intron 35.0%
! GTGAAATTTGATGGTTGTCA+AGG + chr2.2:56021905-56021924 MS.gene058304:CDS 35.0%
! TAAGTGGATTCCATCAGTAT+TGG - chr2.2:56021975-56021994 None:intergenic 35.0%
!! ACTAAGTTTTGGTGCAGAAA+TGG - chr2.2:56021951-56021970 None:intergenic 35.0%
!! AGCTCAAATTTGGTGTTAAG+AGG + chr2.2:56021851-56021870 MS.gene058304:CDS 35.0%
!! GATTTTTGGACCACAGAATT+TGG - chr2.2:56022356-56022375 None:intergenic 35.0%
!! GCTCAAATTTGGTGTTAAGA+GGG + chr2.2:56021852-56021871 MS.gene058304:CDS 35.0%
!! TCAGTATTGGGACTAAGTTT+TGG - chr2.2:56021962-56021981 None:intergenic 35.0%
!!! TACCTTGGGGACTAATTTTT+TGG + chr2.2:56022714-56022733 MS.gene058304:intron 35.0%
!!! TAGTTGATGACCCTTTTTGT+TGG + chr2.2:56023857-56023876 MS.gene058304:CDS 35.0%
AACGCAACCGTGAACATAAA+AGG - chr2.2:56021037-56021056 None:intergenic 40.0%
ACATTCACATCTGAACATCC+AGG - chr2.2:56022168-56022187 None:intergenic 40.0%
AGAATCAAAACATGGCAAGG+AGG + chr2.2:56023571-56023590 MS.gene058304:CDS 40.0%
ATCTTCCTTGCAAACCTCAT+AGG + chr2.2:56021553-56021572 MS.gene058304:CDS 40.0%
ATGTGACATACCTAATTCCG+TGG - chr2.2:56022215-56022234 None:intergenic 40.0%
ATTACCCTATGAGGTTTGCA+AGG - chr2.2:56021561-56021580 None:intergenic 40.0%
CAAATGACTCTAAATGGGAC+AGG + chr2.2:56021498-56021517 MS.gene058304:CDS 40.0%
CCACTTGATTGTTTAAGGGA+AGG + chr2.2:56023916-56023935 MS.gene058304:CDS 40.0%
CCTTCCCTTAAACAATCAAG+TGG - chr2.2:56023919-56023938 None:intergenic 40.0%
CTACAAGCAAAGTGTATTGG+AGG - chr2.2:56023772-56023791 None:intergenic 40.0%
CTGTCGAAATCACATCAGTA+AGG - chr2.2:56023460-56023479 None:intergenic 40.0%
CTTACTTGAAATACCAGTCC+TGG - chr2.2:56020998-56021017 None:intergenic 40.0%
CTTCAAAATCCTTTACGCTG+CGG - chr2.2:56021218-56021237 None:intergenic 40.0%
GAATCCACTTAGGAAACACA+AGG + chr2.2:56021984-56022003 MS.gene058304:CDS 40.0%
GATTGCATTTCAATTGGTCC+TGG + chr2.2:56022147-56022166 MS.gene058304:CDS 40.0%
GCACATCACATGTAACTTCT+AGG - chr2.2:56023380-56023399 None:intergenic 40.0%
GGAGATTCTCCAGGATTATT+TGG - chr2.2:56020797-56020816 None:intergenic 40.0%
GGATATGGATATGGATCAGA+TGG - chr2.2:56020776-56020795 None:intergenic 40.0%
GGTAGCAATCTATTACCTTG+GGG + chr2.2:56022701-56022720 MS.gene058304:intron 40.0%
GTCCAAAAAATTAGTCCCCA+AGG - chr2.2:56022719-56022738 None:intergenic 40.0%
TAATCCTGGAGAATCTCCTT+CGG + chr2.2:56020799-56020818 MS.gene058304:CDS 40.0%
TATGGATCAGATGGAACACT+AGG - chr2.2:56020767-56020786 None:intergenic 40.0%
TCATCCATAATGTGGAAGGA+AGG + chr2.2:56020663-56020682 MS.gene058304:CDS 40.0%
TCCCTTAAACAATCAAGTGG+AGG - chr2.2:56023916-56023935 None:intergenic 40.0%
TCTTCCTTGCAAACCTCATA+GGG + chr2.2:56021554-56021573 MS.gene058304:CDS 40.0%
TGATGTGATTTCGACAGCAT+TGG + chr2.2:56023463-56023482 MS.gene058304:intron 40.0%
TGGATGTTCAGATGTGAATG+TGG + chr2.2:56022167-56022186 MS.gene058304:CDS 40.0%
TGTGACATACCTAATTCCGT+GGG - chr2.2:56022214-56022233 None:intergenic 40.0%
TGTGTACTTCTCATCAACTG+TGG - chr2.2:56023049-56023068 None:intergenic 40.0%
TGTTCAGATGTGAATGTGGA+TGG + chr2.2:56022171-56022190 MS.gene058304:CDS 40.0%
TTAGACAAATGTGGCTGATG+TGG + chr2.2:56021072-56021091 MS.gene058304:intron 40.0%
TTAGGAAACACAAGGGATGT+TGG + chr2.2:56021992-56022011 MS.gene058304:CDS 40.0%
TTGAAGGCAATGGAGAACAA+TGG + chr2.2:56021526-56021545 MS.gene058304:CDS 40.0%
! GATGAGAAGTACACACTTAG+TGG + chr2.2:56023054-56023073 MS.gene058304:intron 40.0%
! GTAAGATCATTTGGAGCAGT+TGG + chr2.2:56020836-56020855 MS.gene058304:CDS 40.0%
AAGGCAATGGAGAACAATGG+TGG + chr2.2:56021529-56021548 MS.gene058304:CDS 45.0%
ACATACCTAATTCCGTGGGT+AGG - chr2.2:56022210-56022229 None:intergenic 45.0%
ACATCGAAAACGCAATCCGA+AGG - chr2.2:56020818-56020837 None:intergenic 45.0%
ACTAGGAGATGTAGGAGGAT+TGG - chr2.2:56020750-56020769 None:intergenic 45.0%
AGGCAATGGAGAACAATGGT+GGG + chr2.2:56021530-56021549 MS.gene058304:CDS 45.0%
AGGCAGACAGTAAAAGCCAA+TGG + chr2.2:56021454-56021473 MS.gene058304:CDS 45.0%
ATAGATTGCTACCTAGGAGC+AGG - chr2.2:56022694-56022713 None:intergenic 45.0%
CACCAGATGGACCAAATTGT+TGG + chr2.2:56021427-56021446 MS.gene058304:CDS 45.0%
CATCCCTTGTGTTTCCTAAG+TGG - chr2.2:56021991-56022010 None:intergenic 45.0%
GATAACTCCACAACCGTTTC+AGG - chr2.2:56023946-56023965 None:intergenic 45.0%
GATGGAACACTAGGAGATGT+AGG - chr2.2:56020758-56020777 None:intergenic 45.0%
GATGTAGGAGGATTGGAAGA+AGG - chr2.2:56020743-56020762 None:intergenic 45.0%
GATTGGAAGAAGGTGGTGAA+GGG - chr2.2:56020733-56020752 None:intergenic 45.0%
GGCTGCAATTATGGTTGCAA+AGG + chr2.2:56021093-56021112 MS.gene058304:intron 45.0%
GGTACCTTCCTTCCACATTA+TGG - chr2.2:56020670-56020689 None:intergenic 45.0%
GTGGTCTGCAACAGTAATAG+AGG + chr2.2:56021192-56021211 MS.gene058304:intron 45.0%
TAGATTGCTACCTAGGAGCA+GGG - chr2.2:56022693-56022712 None:intergenic 45.0%
! AAAACATGGCAAGGAGGTAC+AGG + chr2.2:56023577-56023596 MS.gene058304:CDS 45.0%
! AATACCAGTCCTGGTTTGCA+TGG - chr2.2:56020989-56021008 None:intergenic 45.0%
! CTGCAGAAACTTTTACGCTG+TGG + chr2.2:56021116-56021135 MS.gene058304:intron 45.0%
! TAGGTTTGAGACACATGCGT+GGG - chr2.2:56023509-56023528 None:intergenic 45.0%
! TCATTTGGAGCAGTTGGAGA+TGG + chr2.2:56020842-56020861 MS.gene058304:CDS 45.0%
! TTAGGTTTGAGACACATGCG+TGG - chr2.2:56023510-56023529 None:intergenic 45.0%
!! GGTGCTGGAGAAGTTTTCTT+TGG - chr2.2:56020704-56020723 None:intergenic 45.0%
!! TTTTCTGGTCCATGCAAACC+AGG + chr2.2:56020977-56020996 MS.gene058304:CDS 45.0%
!! TAAGAAAAAAAAAAATTATA+AGG - chr2.2:56021794-56021813 None:intergenic 5.0%
AAGGGAAGGTGAGCCTGAAA+CGG + chr2.2:56023930-56023949 MS.gene058304:CDS 50.0%
AGATTGCTACCTAGGAGCAG+GGG - chr2.2:56022692-56022711 None:intergenic 50.0%
AGGACCATGTGTTAGCCCTA+CGG + chr2.2:56021695-56021714 MS.gene058304:CDS 50.0%
CAATCCGAAGGAGATTCTCC+AGG - chr2.2:56020806-56020825 None:intergenic 50.0%
CGTGGGAATTGTGTACTCCA+AGG - chr2.2:56023492-56023511 None:intergenic 50.0%
GGAACACTAGGAGATGTAGG+AGG - chr2.2:56020755-56020774 None:intergenic 50.0%
GGATTGGAAGAAGGTGGTGA+AGG - chr2.2:56020734-56020753 None:intergenic 50.0%
GGCCAACAATTTGGTCCATC+TGG - chr2.2:56021432-56021451 None:intergenic 50.0%
GGCTGATGTGGCTGCAATTA+TGG + chr2.2:56021084-56021103 MS.gene058304:intron 50.0%
GTAGGAGGATTGGAAGAAGG+TGG - chr2.2:56020740-56020759 None:intergenic 50.0%
GTTACTTGTGCTCCTACCCA+CGG + chr2.2:56022195-56022214 MS.gene058304:CDS 50.0%
TAATAGAGGCCGCAGCGTAA+AGG + chr2.2:56021206-56021225 MS.gene058304:intron 50.0%
TCTGCCTCAGGCCAACAATT+TGG - chr2.2:56021441-56021460 None:intergenic 50.0%
! CAGCTGCATTTAGAGCAGCA+TGG + chr2.2:56020879-56020898 MS.gene058304:CDS 50.0%
! GCAGCTTGTGCAGTTGATTC+AGG + chr2.2:56020905-56020924 MS.gene058304:CDS 50.0%
! GGAGGTACAGGATCAGTAAC+AGG + chr2.2:56023589-56023608 MS.gene058304:CDS 50.0%
! GGTACTTTGATGGCACCAGA+TGG + chr2.2:56021414-56021433 MS.gene058304:CDS 50.0%
! TGACTGAGTTGCGGACTGTT+AGG - chr2.2:56023528-56023547 None:intergenic 50.0%
! TGGACCAAATTGTTGGCCTG+AGG + chr2.2:56021434-56021453 MS.gene058304:CDS 50.0%
! TGGCTTTTACTGTCTGCCTC+AGG - chr2.2:56021453-56021472 None:intergenic 50.0%
! TTCGACAGCATTGGAAGCCT+TGG + chr2.2:56023472-56023491 MS.gene058304:intron 50.0%
AGGTGAGCCTGAAACGGTTG+TGG + chr2.2:56023936-56023955 MS.gene058304:CDS 55.0%
CTCACCGTAGGGCTAACACA+TGG - chr2.2:56021702-56021721 None:intergenic 55.0%
GAAGGTGGTGAAGGGTCACT+AGG - chr2.2:56020725-56020744 None:intergenic 55.0%
GTGCTCCTACCCACGGAATT+AGG + chr2.2:56022202-56022221 MS.gene058304:CDS 55.0%
TGGTCCATGCAAACCAGGAC+TGG + chr2.2:56020982-56021001 MS.gene058304:CDS 55.0%
! CTGTTTTGTCCCCTGCTCCT+AGG + chr2.2:56022680-56022699 MS.gene058304:intron 55.0%
GGTGAAGGGTCACTAGGTGC+TGG - chr2.2:56020719-56020738 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr2.2 gene 56020653 56023987 56020653 ID=MS.gene058304
chr2.2 mRNA 56020653 56023987 56020653 ID=MS.gene058304.t1;Parent=MS.gene058304
chr2.2 exon 56020653 56021003 56020653 ID=MS.gene058304.t1.exon1;Parent=MS.gene058304.t1
chr2.2 CDS 56020653 56021003 56020653 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
chr2.2 exon 56021408 56021575 56021408 ID=MS.gene058304.t1.exon2;Parent=MS.gene058304.t1
chr2.2 CDS 56021408 56021575 56021408 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
chr2.2 exon 56021672 56021716 56021672 ID=MS.gene058304.t1.exon3;Parent=MS.gene058304.t1
chr2.2 CDS 56021672 56021716 56021672 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
chr2.2 exon 56021833 56022040 56021833 ID=MS.gene058304.t1.exon4;Parent=MS.gene058304.t1
chr2.2 CDS 56021833 56022040 56021833 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
chr2.2 exon 56022142 56022223 56022142 ID=MS.gene058304.t1.exon5;Parent=MS.gene058304.t1
chr2.2 CDS 56022142 56022223 56022142 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
chr2.2 exon 56023480 56023987 56023480 ID=MS.gene058304.t1.exon6;Parent=MS.gene058304.t1
chr2.2 CDS 56023480 56023987 56023480 ID=cds.MS.gene058304.t1;Parent=MS.gene058304.t1
Gene Sequence

>MS.gene058304

ATGATTCTTTTCATCCATAATGTGGAAGGAAGGTACCATCACAAGAAACCAAAGAAAACTTCTCCAGCACCTAGTGACCCTTCACCACCTTCTTCCAATCCTCCTACATCTCCTAGTGTTCCATCTGATCCATATCCATATCCAAATAATCCTGGAGAATCTCCTTCGGATTGCGTTTTCGATGTAAGATCATTTGGAGCAGTTGGAGATGGTAATGCTGATGATACAGCTGCATTTAGAGCAGCATGGAAAGCAGCTTGTGCAGTTGATTCAGGAGTTCTTTTAGCTCCTGAAAATTACTGTTTTAAAATCACTTCTACTATCTTTTCTGGTCCATGCAAACCAGGACTGGTATTTCAAGTAAGAATGTTTCTTAAATTTCCTTTTATGTTCACGGTTGCGTTGCGATTCTTTAAATTTTAGACAAATGTGGCTGATGTGGCTGCAATTATGGTTGCAAAGGCTGCAGAAACTTTTACGCTGTGGTCGCAATTGCTGTTATAGACTGCAATTTAGAATCACGATTGTGGTTTTAAATTGTGGTCTGCAACAGTAATAGAGGCCGCAGCGTAAAGGATTTTGAAGTCTCTTCAAAGTCATTGTAACTATAATTTTGACTGCATCAGCTGCATTTTCTCTAATTTATCCGCAATATAAAAGTTTGCAGCGTAATTGGAACTGAAATTTAACATGACCATCATTGTGTAAAATTTAATTTCCTATATAATTAACTAACAAGGTTGAAATATATGTAGATTGATGGTACTTTGATGGCACCAGATGGACCAAATTGTTGGCCTGAGGCAGACAGTAAAAGCCAATGGCTTGTGTTTTATAGACTTGATCAAATGACTCTAAATGGGACAGGAATTATTGAAGGCAATGGAGAACAATGGTGGGATCTTCCTTGCAAACCTCATAGGGTAATAATAATAATTATTATTAAAACTTTATTTTTTTTTATTTATTTATCTAATGTTAAAAACTAAAATTCTAGTAATTTTTCATTTTCATTTCAGAGTCCAAATGGAAAAACTGTATCAGGACCATGTGTTAGCCCTACGGTGAGTAAAATATTCAGAATTTACCTTAACCATTACATCACAGTTTCTTTTTTTTTTTTTTTAAATATGAAATGCCTTATAATTTTTTTTTTTCTTATGAAAAATCTTGTTTATAGATGATAAGGTTCTTCATGAGCTCAAATTTGGTGTTAAGAGGGTTGAAAATACAAAATAGTCCTCAATTCCATGTGAAATTTGATGGTTGTCAAGGAGTATTAATTGATGAATTGTCCATTTCTGCACCAAAACTTAGTCCCAATACTGATGGAATCCACTTAGGAAACACAAGGGATGTTGGAATATATAATTCCTTGATAAGCAATGGTAAATAATAAAGATCATTGTCACACTTTCTCAATATAGAATTTTTATTCTATGGTTTTTTTATTTAATTATGTCACATAATGTTACATTTTATTTAATAGGTGATGATTGCATTTCAATTGGTCCTGGATGTTCAGATGTGAATGTGGATGGTGTTACTTGTGCTCCTACCCACGGAATTAGGTATGTCACATAGTTTCTGTTATTTTAGTTAAATTGGATTGTTAACTATCAATTAATTATGATTAATTTAGGAATAAAATAAAAATAAGCTACAAACTTAGTTGATGGTATTCATATAAATATATACACCCAAATTCTGTGGTCCAAAAATCATTTCAAAAGGCAAAATGAATGAAAAGTGACTTTAACCTGTAGTGTGAGTGTAATAACTCTTGAAGGTGTCTTATAAGATAGCAATTTATCTTCAAGAAAAATAAATAAATAAATAAATAAATAAATAAATAAATAAATAATGGAACATTTTTGTTTGAAATAGAAACCTTTCTAACTTCAAATGATCACAGGATTAATTTTCTTCTTTTAGAGATTTATGTGACTATAGTACTTTAACAGTTGGAAGTTTCAAACTGCCAATCATTAATGTGACAATAGTTTTTGTTCTTCATTTCTGTAATTCTGTTTTGTCCCCTGCTCCTAGGTAGCAATCTATTACCTTGGGGACTAATTTTTTGGACATGACTTTTAAAGATGTTGATTTTGTAAAACTTATTAACATAAAATTGAGTTTAAAGTAAACTGATTTATATTCATATGATTTTATTTTAAAATCAAAGTTTATTATTTTAGAGGTATAAAACTGATTTTATATGTTTGGATGTTATAGAGTATAATTGATATTGTCTCAAAAATTAACTCTAACTTAAGCTAGAATTTTGAGCTTCTAACTCTCCAATTGTTTTTAAGATTAAGTTTTTTTTTCAATTCACTTTTACATAAATATATACAACAAAAAATAAATTTACATTCAACGCACTACTTATACCACAGTTGATGAGAAGTACACACTTAGTGGACTAAACATGTTATAACATCTACGAAAATGTCTGATACTAGAGACCAAAATAAACTTGAAAAGAAAAAAAAAACTCTGAAAACATTTACAATCTTTCATAAAATAATTACATAATTGCAATCAGTGTCATAGATTAGAAGTTGTTTTATGTTGTATGGCTTGATTCCAATTATGTAATCATTTTATGAAAAATTGTAAATGTTCATTGAGTTATGAGAGTTCTTTTTTTTCTTCAAATTTATTTTGGTCTCTAGTATACATCTTCTTAGAATTATTTTGGTCTCTAGTATGAGACATCTTCCTAGAAGTTACATGTGATGTGCTATTTTGGAACCTATAGAATTTTGAGTAGCAAGCTTCTATGTAGCTATAATTAAAAACCTTACTGATGTGATTTCGACAGCATTGGAAGCCTTGGAGTACACAATTCCCACGCATGTGTCTCAAACCTAACAGTCCGCAACTCAGTCATAAAAGAATCAGACAATGGACTAAGAATCAAAACATGGCAAGGAGGTACAGGATCAGTAACAGGTTTAACATTTGACAACATCCAAATGGAGAATGTAAGAAACTGTATCAACATAGACCAATTCTACTGTTTATCAAAAGAGTGTATGAACCAAACATCAGCAGTGTATGTAAACAATATCTCCTACAGAAAAATAAAGGGTACATATGATGTGAGAACACCTCCAATACACTTTGCTTGTAGTGACACTGTTGCTTGCACAAACATCACACTCTCTGAGATTGAACTTTTACCTTATGAAGGTGAATTAGTTGATGACCCTTTTTGTTGGAATGCTTATGGAAGACAAGAGACTTTGACTATTCCTCCACTTGATTGTTTAAGGGAAGGTGAGCCTGAAACGGTTGTGGAGTTATCTGAATATGAATGCAATAATTAA
Protein sequence

>MS.gene058304.t1

MILFIHNVEGRYHHKKPKKTSPAPSDPSPPSSNPPTSPSVPSDPYPYPNNPGESPSDCVFDVRSFGAVGDGNADDTAAFRAAWKAACAVDSGVLLAPENYCFKITSTIFSGPCKPGLIDGTLMAPDGPNCWPEADSKSQWLVFYRLDQMTLNGTGIIEGNGEQWWDLPCKPHRSPNGKTVSGPCVSPTMIRFFMSSNLVLRGLKIQNSPQFHVKFDGCQGVLIDELSISAPKLSPNTDGIHLGNTRDVGIYNSLISNGDDCISIGPGCSDVNVDGVTCAPTHGISIGSLGVHNSHACVSNLTVRNSVIKESDNGLRIKTWQGGTGSVTGLTFDNIQMENVRNCINIDQFYCLSKECMNQTSAVYVNNISYRKIKGTYDVRTPPIHFACSDTVACTNITLSEIELLPYEGELVDDPFCWNAYGRQETLTIPPLDCLREGEPETVVELSEYECNN