Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56098.t1 | XP_013467029.1 | 94.1 | 455 | 8 | 1 | 1 | 436 | 1 | 455 | 5.10E-239 | 836.6 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56098.t1 | Q9UKZ1 | 44.8 | 455 | 208 | 12 | 6 | 436 | 63 | 498 | 8.4e-81 | 302.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56098.t1 | A0A072VGA3 | 94.1 | 455 | 8 | 1 | 1 | 436 | 1 | 455 | 3.7e-239 | 836.6 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053333 | MS.gene56098 | 0.881974 | 1.54E-70 | -1.69E-46 |
| MS.gene053334 | MS.gene56098 | 0.831674 | 1.46E-55 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56098.t1 | MTR_1g041405 | 94.066 | 455 | 8 | 1 | 1 | 436 | 1 | 455 | 0.0 | 867 |
| MS.gene56098.t1 | MTR_1g041405 | 92.000 | 350 | 9 | 1 | 1 | 331 | 1 | 350 | 0.0 | 652 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56098.t1 | AT5G18420 | 57.407 | 432 | 183 | 1 | 6 | 436 | 9 | 440 | 0.0 | 520 |
| MS.gene56098.t1 | AT5G18420 | 57.275 | 433 | 183 | 1 | 6 | 436 | 9 | 441 | 0.0 | 519 |
| MS.gene56098.t1 | AT5G18420 | 57.044 | 433 | 181 | 2 | 6 | 436 | 9 | 438 | 1.81e-180 | 511 |
| MS.gene56098.t1 | AT5G18420 | 48.160 | 326 | 169 | 0 | 6 | 331 | 9 | 334 | 2.26e-113 | 337 |
Find 98 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAATCTGTATCACAATT+TGG | 0.161000 | 1.1:-25430969 | None:intergenic |
| CACTGCTTCATCTTTATTTC+CGG | 0.181836 | 1.1:-25431737 | None:intergenic |
| CCCATTTCAGCAATTCCTTT+TGG | 0.204315 | 1.1:+25433010 | MS.gene56098:intron |
| GAGAATTTCAACTGCAATTA+GGG | 0.204802 | 1.1:-25433249 | None:intergenic |
| TGAGAATTTCAACTGCAATT+AGG | 0.207484 | 1.1:-25433250 | None:intergenic |
| AGCATACCTCTTTGCCATTA+TGG | 0.211924 | 1.1:-25430118 | None:intergenic |
| ATTCGGAACAACATTATTAA+TGG | 0.258078 | 1.1:+25434893 | MS.gene56098:CDS |
| AAGGATTTGAAGCCGGATTC+TGG | 0.267414 | 1.1:-25428990 | None:intergenic |
| TAATAATGTTGTTCCGAATC+AGG | 0.283055 | 1.1:-25434889 | None:intergenic |
| ATGAATCTGTATCACAATTT+GGG | 0.286687 | 1.1:-25430968 | None:intergenic |
| CTTTACAGTACTTGTCAATA+TGG | 0.293533 | 1.1:+25433397 | MS.gene56098:CDS |
| GCACGTACCGGCAACTTTCT+TGG | 0.303493 | 1.1:-25433077 | None:intergenic |
| GCATACCTCTTTGCCATTAT+GGG | 0.303885 | 1.1:-25430117 | None:intergenic |
| TTACCTCCCCATCCTCCTTC+AGG | 0.308872 | 1.1:+25427371 | MS.gene56098:CDS |
| TCTTTAAACAAATGATGATA+TGG | 0.317716 | 1.1:-25430906 | None:intergenic |
| GCGTTTATTCTGCAACTATT+AGG | 0.329631 | 1.1:+25430083 | MS.gene56098:CDS |
| ATAAGCTCGGGGTAGTCATT+AGG | 0.333794 | 1.1:-25431998 | None:intergenic |
| ATTTAGACTTCTCAAGTCAT+TGG | 0.379051 | 1.1:+25435869 | MS.gene56098:CDS |
| CCAAGTTTAGGCTGTGTTCC+AGG | 0.390651 | 1.1:-25431712 | None:intergenic |
| CCAAGGCTCCCGATACTTGA+TGG | 0.408616 | 1.1:+25431823 | MS.gene56098:CDS |
| ATAATCACCTCTTGCTGTAC+AGG | 0.414225 | 1.1:-25432106 | None:intergenic |
| GGCTTATTCATCCCAGAATC+CGG | 0.414456 | 1.1:+25428978 | MS.gene56098:CDS |
| TATCATCATTTGTTTAAAGA+TGG | 0.424584 | 1.1:+25430909 | MS.gene56098:CDS |
| TAAGCTCGGGGTAGTCATTA+GGG | 0.429964 | 1.1:-25431997 | None:intergenic |
| CCACAGTGATATACAACCTT+TGG | 0.432590 | 1.1:-25433047 | None:intergenic |
| ATGACTACCCCGAGCTTATG+TGG | 0.434285 | 1.1:+25432002 | MS.gene56098:CDS |
| CTAGTAGAGGGGCTGCAGTA+AGG | 0.443465 | 1.1:+25432047 | MS.gene56098:CDS |
| AGGATTTGAAGCCGGATTCT+GGG | 0.445204 | 1.1:-25428989 | None:intergenic |
| TTATTTCCGGTGCCAAGTTT+AGG | 0.450510 | 1.1:-25431724 | None:intergenic |
| AGGATAAATACATGCAGAAC+AGG | 0.450662 | 1.1:+25434834 | MS.gene56098:intron |
| CGTAAATACCTGAAGGAGGA+TGG | 0.451873 | 1.1:-25427379 | None:intergenic |
| ACTGACTCCAAGAAAGTTGC+CGG | 0.464679 | 1.1:+25433070 | MS.gene56098:CDS |
| CCTGGAACACAGCCTAAACT+TGG | 0.467985 | 1.1:+25431712 | MS.gene56098:CDS |
| ATTCAATGGAAGTTGTGAAC+AGG | 0.470837 | 1.1:+25433432 | MS.gene56098:CDS |
| GGACATGAGTCTACATTCAA+TGG | 0.474092 | 1.1:+25433418 | MS.gene56098:CDS |
| TGTTCCGAATCAGGCTTTGC+AGG | 0.476418 | 1.1:-25434880 | None:intergenic |
| TGGAAGGATTGAACCCTCAC+TGG | 0.483292 | 1.1:+25431785 | MS.gene56098:CDS |
| CTTATTGTCAAATATTTCAC+TGG | 0.488068 | 1.1:+25431765 | MS.gene56098:CDS |
| TCAAGTATCGGGAGCCTTGG+TGG | 0.495802 | 1.1:-25431820 | None:intergenic |
| CTTATGAAAGGATTTGAAGC+CGG | 0.499316 | 1.1:-25428997 | None:intergenic |
| GGTGTTGATGTATCCCATAA+TGG | 0.499372 | 1.1:+25430104 | MS.gene56098:CDS |
| CGCTATTAATCTCTCGTTGG+TGG | 0.499459 | 1.1:-25428938 | None:intergenic |
| CTTAATTGCAAAAGCTCAGA+AGG | 0.503092 | 1.1:+25432072 | MS.gene56098:CDS |
| AATAGGTTTGATCAGCGACC+TGG | 0.508059 | 1.1:+25431694 | MS.gene56098:intron |
| ATCGGGAGCCTTGGTGGAAG+AGG | 0.511773 | 1.1:-25431814 | None:intergenic |
| TTACAGGCTTCCTGTCATGA+AGG | 0.511927 | 1.1:+25430041 | MS.gene56098:intron |
| AATGCATACTCTGATATGTC+AGG | 0.512674 | 1.1:-25433290 | None:intergenic |
| TTGTCAAATATTTCACTGGA+AGG | 0.512810 | 1.1:+25431769 | MS.gene56098:CDS |
| TAGTAGAGGGGCTGCAGTAA+GGG | 0.513261 | 1.1:+25432048 | MS.gene56098:CDS |
| ATACCTCGCCATCAAGTATC+GGG | 0.514225 | 1.1:-25431831 | None:intergenic |
| TACAGGCTTCCTGTCATGAA+GGG | 0.514425 | 1.1:+25430042 | MS.gene56098:intron |
| CCATCAAGTATCGGGAGCCT+TGG | 0.519598 | 1.1:-25431823 | None:intergenic |
| ACCATTGTCCCACATAAGCT+CGG | 0.522735 | 1.1:-25432011 | None:intergenic |
| CCATTGTCCCACATAAGCTC+GGG | 0.525577 | 1.1:-25432010 | None:intergenic |
| AATACCTCGCCATCAAGTAT+CGG | 0.543271 | 1.1:-25431832 | None:intergenic |
| TTTATGATAAAGCTTATGAA+AGG | 0.545065 | 1.1:-25429009 | None:intergenic |
| GATCCCGTGCAAGCTCCAAA+AGG | 0.558073 | 1.1:-25433025 | None:intergenic |
| GTAAATACCTGAAGGAGGAT+GGG | 0.560127 | 1.1:-25427378 | None:intergenic |
| ACTGCAGTTTCTTAAACAGT+CGG | 0.560584 | 1.1:+25430676 | MS.gene56098:intron |
| GGAGCTTGCACGGGATCCAA+AGG | 0.564350 | 1.1:+25433031 | MS.gene56098:CDS |
| TGCTTCAACTGATCTAGTGA+AGG | 0.568307 | 1.1:-25430855 | None:intergenic |
| AATAAAGATGAAGCAGTGGT+TGG | 0.571459 | 1.1:+25431742 | MS.gene56098:CDS |
| AGAGTGTAATGGCGAGAGTG+AGG | 0.572224 | 1.1:-25427342 | None:intergenic |
| ATCATCCTGTGTGAACATCA+AGG | 0.588770 | 1.1:+25433514 | MS.gene56098:CDS |
| TAAATACCTGAAGGAGGATG+GGG | 0.590079 | 1.1:-25427377 | None:intergenic |
| AAACGCTATTAATCTCTCGT+TGG | 0.594956 | 1.1:-25428941 | None:intergenic |
| ATGGCGAGAGTGAGGAATGG+TGG | 0.595698 | 1.1:-25427334 | None:intergenic |
| TGAATCTGTATCACAATTTG+GGG | 0.599720 | 1.1:-25430967 | None:intergenic |
| TATTCTCTATCAGGAACTGG+TGG | 0.600122 | 1.1:+25433217 | MS.gene56098:intron |
| ATGTGCGTTGATACTAGTAG+AGG | 0.600614 | 1.1:+25432034 | MS.gene56098:CDS |
| AGCGTTTGCTCTGCTAGTTG+AGG | 0.605843 | 1.1:+25428957 | MS.gene56098:CDS |
| TGACTACCCCGAGCTTATGT+GGG | 0.608574 | 1.1:+25432003 | MS.gene56098:CDS |
| TCTTGCACCTGTACAGCAAG+AGG | 0.609228 | 1.1:+25432099 | MS.gene56098:CDS |
| ACACAGCCTAAACTTGGCAC+CGG | 0.610220 | 1.1:+25431718 | MS.gene56098:CDS |
| CCCGAGCTTATGTGGGACAA+TGG | 0.613988 | 1.1:+25432010 | MS.gene56098:CDS |
| TGTGCGTTGATACTAGTAGA+GGG | 0.616078 | 1.1:+25432035 | MS.gene56098:CDS |
| CGGAAATAAAGATGAAGCAG+TGG | 0.618683 | 1.1:+25431738 | MS.gene56098:CDS |
| GGTAAGAACAGAGAGTGTAA+TGG | 0.620404 | 1.1:-25427353 | None:intergenic |
| GCTTCAACTGATCTAGTGAA+GGG | 0.620688 | 1.1:-25430854 | None:intergenic |
| GCTCCCGATACTTGATGGCG+AGG | 0.628235 | 1.1:+25431828 | MS.gene56098:CDS |
| TGTATCCCATAATGGCAAAG+AGG | 0.640235 | 1.1:+25430112 | MS.gene56098:CDS |
| AGAATTTCAACTGCAATTAG+GGG | 0.640657 | 1.1:-25433248 | None:intergenic |
| ATACCTGAAGGAGGATGGGG+AGG | 0.646330 | 1.1:-25427374 | None:intergenic |
| TCAGCGAGGATCTCATTGAG+AGG | 0.650902 | 1.1:-25427303 | None:intergenic |
| GGATTAGACCTCTTCCACCA+AGG | 0.651322 | 1.1:+25431806 | MS.gene56098:CDS |
| AAGTAACTTACATTCAGTGA+CGG | 0.653960 | 1.1:-25430725 | None:intergenic |
| TACATGTGAATGAATTCTGA+CGG | 0.655091 | 1.1:-25433476 | None:intergenic |
| GAAGAGGTCTAATCCAGTGA+GGG | 0.663114 | 1.1:-25431798 | None:intergenic |
| AAATCGTAAATACCTGAAGG+AGG | 0.666534 | 1.1:-25427383 | None:intergenic |
| TTAATTGCAAAAGCTCAGAA+GGG | 0.683329 | 1.1:+25432073 | MS.gene56098:CDS |
| GTAATGGCGAGAGTGAGGAA+TGG | 0.688538 | 1.1:-25427337 | None:intergenic |
| GTGCGTTGATACTAGTAGAG+GGG | 0.694698 | 1.1:+25432036 | MS.gene56098:CDS |
| GGAAGAGGTCTAATCCAGTG+AGG | 0.700569 | 1.1:-25431799 | None:intergenic |
| CATTGTCCCACATAAGCTCG+GGG | 0.705703 | 1.1:-25432009 | None:intergenic |
| AAGTACCTTGATGTTCACAC+AGG | 0.718063 | 1.1:-25433519 | None:intergenic |
| CCAAAGGTTGTATATCACTG+TGG | 0.722141 | 1.1:+25433047 | MS.gene56098:CDS |
| TGGTGGATTTGAATTCAGCG+AGG | 0.746032 | 1.1:-25427317 | None:intergenic |
| GAATCTGTATCACAATTTGG+GGG | 0.804690 | 1.1:-25430966 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.1 | gene | 25427234 | 25435896 | 25427234 | ID=MS.gene56098 |
| chr1.1 | mRNA | 25427234 | 25435896 | 25427234 | ID=MS.gene56098.t1;Parent=MS.gene56098 |
| chr1.1 | exon | 25427234 | 25427392 | 25427234 | ID=MS.gene56098.t1.exon1;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25427234 | 25427392 | 25427234 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25428922 | 25429032 | 25428922 | ID=MS.gene56098.t1.exon2;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25428922 | 25429032 | 25428922 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25430047 | 25430133 | 25430047 | ID=MS.gene56098.t1.exon3;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25430047 | 25430133 | 25430047 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25430683 | 25430736 | 25430683 | ID=MS.gene56098.t1.exon4;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25430683 | 25430736 | 25430683 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25430849 | 25430994 | 25430849 | ID=MS.gene56098.t1.exon5;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25430849 | 25430994 | 25430849 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25431699 | 25431849 | 25431699 | ID=MS.gene56098.t1.exon6;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25431699 | 25431849 | 25431699 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25431983 | 25432120 | 25431983 | ID=MS.gene56098.t1.exon7;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25431983 | 25432120 | 25431983 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25433020 | 25433091 | 25433020 | ID=MS.gene56098.t1.exon8;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25433020 | 25433091 | 25433020 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25433230 | 25433303 | 25433230 | ID=MS.gene56098.t1.exon9;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25433230 | 25433303 | 25433230 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25433394 | 25433535 | 25433394 | ID=MS.gene56098.t1.exon10;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25433394 | 25433535 | 25433394 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25434836 | 25434914 | 25434836 | ID=MS.gene56098.t1.exon11;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25434836 | 25434914 | 25434836 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| chr1.1 | exon | 25435799 | 25435896 | 25435799 | ID=MS.gene56098.t1.exon12;Parent=MS.gene56098.t1 |
| chr1.1 | CDS | 25435799 | 25435896 | 25435799 | ID=cds.MS.gene56098.t1;Parent=MS.gene56098.t1 |
| Gene Sequence |
| Protein sequence |
