Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053333.t1 | XP_013467029.1 | 93.6 | 423 | 8 | 1 | 1 | 404 | 1 | 423 | 9.00E-222 | 779.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053333.t1 | Q9UKZ1 | 41.6 | 423 | 204 | 12 | 6 | 404 | 63 | 466 | 3.5e-65 | 250.4 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053333.t1 | A0A072VGA3 | 93.6 | 423 | 8 | 1 | 1 | 404 | 1 | 423 | 6.5e-222 | 779.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene053333 | MS.gene053334 | 0.879665 | 1.03E-69 | -1.69E-46 |
| MS.gene053333 | MS.gene063253 | 0.910027 | 2.96E-82 | -1.69E-46 |
| MS.gene053333 | MS.gene56098 | 0.881974 | 1.54E-70 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053333.t1 | MTR_1g041405 | 93.617 | 423 | 8 | 1 | 1 | 404 | 1 | 423 | 0.0 | 803 |
| MS.gene053333.t1 | MTR_1g041405 | 92.000 | 350 | 9 | 1 | 1 | 331 | 1 | 350 | 0.0 | 651 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene053333.t1 | AT5G18420 | 55.000 | 400 | 179 | 1 | 6 | 404 | 9 | 408 | 1.01e-161 | 462 |
| MS.gene053333.t1 | AT5G18420 | 54.863 | 401 | 179 | 1 | 6 | 404 | 9 | 409 | 1.47e-161 | 461 |
| MS.gene053333.t1 | AT5G18420 | 54.613 | 401 | 177 | 2 | 6 | 404 | 9 | 406 | 2.39e-158 | 453 |
| MS.gene053333.t1 | AT5G18420 | 48.160 | 326 | 169 | 0 | 6 | 331 | 9 | 334 | 7.50e-114 | 337 |
Find 97 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GATGAATCTGTATCACAATT+TGG | 0.161000 | 1.4:-25924926 | None:intergenic |
| CACTGCTTCATCTTTATTTC+CGG | 0.181836 | 1.4:-25925694 | None:intergenic |
| AAAATACCTCTTTGCCATTA+TGG | 0.183131 | 1.4:-25924075 | None:intergenic |
| CCCATTTCAGCAATTCCTTT+TGG | 0.204315 | 1.4:+25926981 | MS.gene053333:intron |
| GAGAATTTCAACTGCAATTA+GGG | 0.204802 | 1.4:-25927220 | None:intergenic |
| TGAGAATTTCAACTGCAATT+AGG | 0.207484 | 1.4:-25927221 | None:intergenic |
| ATTCGGAACAACATTATTAA+TGG | 0.258078 | 1.4:+25928869 | MS.gene053333:CDS |
| AAGGATTTGAAGCCGGATTC+TGG | 0.267414 | 1.4:-25922948 | None:intergenic |
| TAATAATGTTGTTCCGAATC+AGG | 0.283055 | 1.4:-25928865 | None:intergenic |
| ATGAATCTGTATCACAATTT+GGG | 0.286687 | 1.4:-25924925 | None:intergenic |
| AAATACCTCTTTGCCATTAT+GGG | 0.291176 | 1.4:-25924074 | None:intergenic |
| ACACGTACCGGCAACTTTCT+TGG | 0.293022 | 1.4:-25927048 | None:intergenic |
| CTTTACAGTACTTGTCAATA+TGG | 0.293533 | 1.4:+25927368 | MS.gene053333:CDS |
| TTACCTCCCCATCCTCCTTC+AGG | 0.308872 | 1.4:+25921296 | MS.gene053333:CDS |
| TCTTTAAACAAATGATGATA+TGG | 0.317716 | 1.4:-25924863 | None:intergenic |
| GCGTTTATTCTGCAACTATT+AGG | 0.329631 | 1.4:+25924040 | MS.gene053333:CDS |
| ATAAGCTCGGGGTAGTCATT+AGG | 0.333794 | 1.4:-25925955 | None:intergenic |
| CCAAGTTTAGGCTGTGTTCC+AGG | 0.390651 | 1.4:-25925669 | None:intergenic |
| CCAAGGCTCCCGATACTTGA+TGG | 0.408616 | 1.4:+25925780 | MS.gene053333:CDS |
| ATAATCACCTCTTGCTGTAC+AGG | 0.414225 | 1.4:-25926063 | None:intergenic |
| GGCTTATTCATCCCAGAATC+CGG | 0.414456 | 1.4:+25922936 | MS.gene053333:CDS |
| TATCATCATTTGTTTAAAGA+TGG | 0.424584 | 1.4:+25924866 | MS.gene053333:CDS |
| TAAGCTCGGGGTAGTCATTA+GGG | 0.429964 | 1.4:-25925954 | None:intergenic |
| CCACAGTGATATACAACCTT+TGG | 0.432590 | 1.4:-25927018 | None:intergenic |
| ATGACTACCCCGAGCTTATG+TGG | 0.434285 | 1.4:+25925959 | MS.gene053333:CDS |
| CTAGTAGAGGGGCTGCAGTA+AGG | 0.443465 | 1.4:+25926004 | MS.gene053333:CDS |
| AGGATTTGAAGCCGGATTCT+GGG | 0.445204 | 1.4:-25922947 | None:intergenic |
| TTATTTCCGGTGCCAAGTTT+AGG | 0.450510 | 1.4:-25925681 | None:intergenic |
| AGGATAAATACATGCAGAAC+AGG | 0.450662 | 1.4:+25928810 | MS.gene053333:intron |
| CGTAAATACCTGAAGGAGGA+TGG | 0.451873 | 1.4:-25921304 | None:intergenic |
| ACTGACTCCAAGAAAGTTGC+CGG | 0.464679 | 1.4:+25927041 | MS.gene053333:CDS |
| CCTGGAACACAGCCTAAACT+TGG | 0.467985 | 1.4:+25925669 | MS.gene053333:CDS |
| ATTCAATGGAAGTTGTGAAC+AGG | 0.470837 | 1.4:+25927403 | MS.gene053333:CDS |
| GGACATGAGTCTACATTCAA+TGG | 0.474092 | 1.4:+25927389 | MS.gene053333:CDS |
| TGTTCCGAATCAGGCTTTGC+AGG | 0.476418 | 1.4:-25928856 | None:intergenic |
| TGGAAGGATTGAACCCTCAC+TGG | 0.483292 | 1.4:+25925742 | MS.gene053333:CDS |
| CTTATTGTCAAATATTTCAC+TGG | 0.488068 | 1.4:+25925722 | MS.gene053333:CDS |
| TCAAGTATCGGGAGCCTTGG+TGG | 0.495802 | 1.4:-25925777 | None:intergenic |
| CTTATGAAAGGATTTGAAGC+CGG | 0.499316 | 1.4:-25922955 | None:intergenic |
| GGTGTTGATGTATCCCATAA+TGG | 0.499372 | 1.4:+25924061 | MS.gene053333:CDS |
| CGCTATTAATCTCTCGTTGG+TGG | 0.499459 | 1.4:-25922896 | None:intergenic |
| CTTAATTGCAAAAGCTCAGA+AGG | 0.503092 | 1.4:+25926029 | MS.gene053333:CDS |
| AATAGGTTTGATCAGCGACC+TGG | 0.508059 | 1.4:+25925651 | MS.gene053333:intron |
| ATCGGGAGCCTTGGTGGAAG+AGG | 0.511773 | 1.4:-25925771 | None:intergenic |
| TTACAGGCTTCCTGTCATGA+AGG | 0.511927 | 1.4:+25923998 | MS.gene053333:intron |
| AATGCATACTCTGATATGTC+AGG | 0.512674 | 1.4:-25927261 | None:intergenic |
| TTGTCAAATATTTCACTGGA+AGG | 0.512810 | 1.4:+25925726 | MS.gene053333:CDS |
| TAGTAGAGGGGCTGCAGTAA+GGG | 0.513261 | 1.4:+25926005 | MS.gene053333:CDS |
| ATACCTCGCCATCAAGTATC+GGG | 0.514225 | 1.4:-25925788 | None:intergenic |
| TACAGGCTTCCTGTCATGAA+GGG | 0.514425 | 1.4:+25923999 | MS.gene053333:intron |
| CCATCAAGTATCGGGAGCCT+TGG | 0.519598 | 1.4:-25925780 | None:intergenic |
| ACCATTGTCCCACATAAGCT+CGG | 0.522735 | 1.4:-25925968 | None:intergenic |
| CCATTGTCCCACATAAGCTC+GGG | 0.525577 | 1.4:-25925967 | None:intergenic |
| AATACCTCGCCATCAAGTAT+CGG | 0.543271 | 1.4:-25925789 | None:intergenic |
| TTTATGATAAAGCTTATGAA+AGG | 0.545065 | 1.4:-25922967 | None:intergenic |
| GATCCCGTGCAAGCTCCAAA+AGG | 0.558073 | 1.4:-25926996 | None:intergenic |
| GTAAATACCTGAAGGAGGAT+GGG | 0.560127 | 1.4:-25921303 | None:intergenic |
| ACTGCAGTTTCTTAAACAGT+CGG | 0.560584 | 1.4:+25924633 | MS.gene053333:intron |
| GGAGCTTGCACGGGATCCAA+AGG | 0.564350 | 1.4:+25927002 | MS.gene053333:CDS |
| TGCTTCAACTGATCTAGTGA+AGG | 0.568307 | 1.4:-25924812 | None:intergenic |
| AATAAAGATGAAGCAGTGGT+TGG | 0.571459 | 1.4:+25925699 | MS.gene053333:CDS |
| AGAGTGTAATGGCGAGAGTG+AGG | 0.572224 | 1.4:-25921267 | None:intergenic |
| ATCATCCTGTGTGAACATCA+AGG | 0.588770 | 1.4:+25927485 | MS.gene053333:CDS |
| TAAATACCTGAAGGAGGATG+GGG | 0.590079 | 1.4:-25921302 | None:intergenic |
| AAACGCTATTAATCTCTCGT+TGG | 0.594956 | 1.4:-25922899 | None:intergenic |
| ATGGCGAGAGTGAGGAATGG+TGG | 0.595698 | 1.4:-25921259 | None:intergenic |
| TGAATCTGTATCACAATTTG+GGG | 0.599720 | 1.4:-25924924 | None:intergenic |
| TATTCTCTATCAGGAACTGG+TGG | 0.600122 | 1.4:+25927188 | MS.gene053333:intron |
| ATGTGCGTTGATACTAGTAG+AGG | 0.600614 | 1.4:+25925991 | MS.gene053333:CDS |
| AGCGTTTGCTCTGCTAGTTG+AGG | 0.605843 | 1.4:+25922915 | MS.gene053333:CDS |
| TGACTACCCCGAGCTTATGT+GGG | 0.608574 | 1.4:+25925960 | MS.gene053333:CDS |
| TCTTGCACCTGTACAGCAAG+AGG | 0.609228 | 1.4:+25926056 | MS.gene053333:CDS |
| ACACAGCCTAAACTTGGCAC+CGG | 0.610220 | 1.4:+25925675 | MS.gene053333:CDS |
| CCCGAGCTTATGTGGGACAA+TGG | 0.613988 | 1.4:+25925967 | MS.gene053333:CDS |
| TGTGCGTTGATACTAGTAGA+GGG | 0.616078 | 1.4:+25925992 | MS.gene053333:CDS |
| CGGAAATAAAGATGAAGCAG+TGG | 0.618683 | 1.4:+25925695 | MS.gene053333:CDS |
| GGTAAGAACAGAGAGTGTAA+TGG | 0.620404 | 1.4:-25921278 | None:intergenic |
| GCTTCAACTGATCTAGTGAA+GGG | 0.620688 | 1.4:-25924811 | None:intergenic |
| GCTCCCGATACTTGATGGCG+AGG | 0.628235 | 1.4:+25925785 | MS.gene053333:CDS |
| TGTATCCCATAATGGCAAAG+AGG | 0.640235 | 1.4:+25924069 | MS.gene053333:CDS |
| AGAATTTCAACTGCAATTAG+GGG | 0.640657 | 1.4:-25927219 | None:intergenic |
| ATACCTGAAGGAGGATGGGG+AGG | 0.646330 | 1.4:-25921299 | None:intergenic |
| TCAGCGAGGATCTCATTGAG+AGG | 0.650902 | 1.4:-25921228 | None:intergenic |
| GGATTAGACCTCTTCCACCA+AGG | 0.651322 | 1.4:+25925763 | MS.gene053333:CDS |
| AAGTAACTTACATTCAGTGA+CGG | 0.653960 | 1.4:-25924682 | None:intergenic |
| TACATGTGAATGAATTCTGA+CGG | 0.655091 | 1.4:-25927447 | None:intergenic |
| GAAGAGGTCTAATCCAGTGA+GGG | 0.663114 | 1.4:-25925755 | None:intergenic |
| AAATCGTAAATACCTGAAGG+AGG | 0.666534 | 1.4:-25921308 | None:intergenic |
| TTAATTGCAAAAGCTCAGAA+GGG | 0.683329 | 1.4:+25926030 | MS.gene053333:CDS |
| GTAATGGCGAGAGTGAGGAA+TGG | 0.688538 | 1.4:-25921262 | None:intergenic |
| GTGCGTTGATACTAGTAGAG+GGG | 0.694698 | 1.4:+25925993 | MS.gene053333:CDS |
| GGAAGAGGTCTAATCCAGTG+AGG | 0.700569 | 1.4:-25925756 | None:intergenic |
| CATTGTCCCACATAAGCTCG+GGG | 0.705703 | 1.4:-25925966 | None:intergenic |
| AAGTACCTTGATGTTCACAC+AGG | 0.718063 | 1.4:-25927490 | None:intergenic |
| CCAAAGGTTGTATATCACTG+TGG | 0.722141 | 1.4:+25927018 | MS.gene053333:CDS |
| TGGTGGATTTGAATTCAGCG+AGG | 0.746032 | 1.4:-25921242 | None:intergenic |
| GAATCTGTATCACAATTTGG+GGG | 0.804690 | 1.4:-25924923 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr1.4 | gene | 25921159 | 25928890 | 25921159 | ID=MS.gene053333 |
| chr1.4 | mRNA | 25921159 | 25928890 | 25921159 | ID=MS.gene053333.t1;Parent=MS.gene053333 |
| chr1.4 | exon | 25921159 | 25921317 | 25921159 | ID=MS.gene053333.t1.exon1;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25921159 | 25921317 | 25921159 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25922880 | 25922990 | 25922880 | ID=MS.gene053333.t1.exon2;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25922880 | 25922990 | 25922880 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25924004 | 25924090 | 25924004 | ID=MS.gene053333.t1.exon3;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25924004 | 25924090 | 25924004 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25924640 | 25924693 | 25924640 | ID=MS.gene053333.t1.exon4;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25924640 | 25924693 | 25924640 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25924806 | 25924951 | 25924806 | ID=MS.gene053333.t1.exon5;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25924806 | 25924951 | 25924806 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25925656 | 25925806 | 25925656 | ID=MS.gene053333.t1.exon6;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25925656 | 25925806 | 25925656 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25925940 | 25926077 | 25925940 | ID=MS.gene053333.t1.exon7;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25925940 | 25926077 | 25925940 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25926991 | 25927062 | 25926991 | ID=MS.gene053333.t1.exon8;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25926991 | 25927062 | 25926991 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25927201 | 25927274 | 25927201 | ID=MS.gene053333.t1.exon9;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25927201 | 25927274 | 25927201 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25927365 | 25927506 | 25927365 | ID=MS.gene053333.t1.exon10;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25927365 | 25927506 | 25927365 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| chr1.4 | exon | 25928812 | 25928890 | 25928812 | ID=MS.gene053333.t1.exon11;Parent=MS.gene053333.t1 |
| chr1.4 | CDS | 25928812 | 25928890 | 25928812 | ID=cds.MS.gene053333.t1;Parent=MS.gene053333.t1 |
| Gene Sequence |
| Protein sequence |
