AlfalfaGEDB | MS.gene56241

M. sativa cultivar XinJiangDaYe / MS.gene56241

KEGG: -
GO: GO:0003674 GO:0004857 GO:0030234 GO:0098772
PFAM: PF04043.18

Nr:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56241.t1	XP_003598232.1	87.2	227	29	0	1	227	1	227	7.50E-101	376.7

Swissprot:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56241.t1	Q9SI74	41.2	160	88	3	66	223	147	302	1.9e-23	110.9

Trembl:

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56241.t1	G7IYI6	87.2	227	29	0	1	227	1	227	5.4e-101	376.7

TFs/TRs:

Gene ID	Type	Classification

Protein Kinases:

Gene ID	Type	Classification

Network:

Co-expression Network:

Gene1	Gene2	correlation coefficient	p_value	FDR

PPI:

Gene1	Gene2	Type
MS.gene21143	MS.gene56241	PPI
MS.gene56241	MS.gene011497	PPI
MS.gene62864	MS.gene56241	PPI
MS.gene015461	MS.gene56241	PPI
MS.gene025785	MS.gene56241	PPI
MS.gene09946	MS.gene56241	PPI
MS.gene56241	MS.gene71906	PPI

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56241.t1	MTR_3g008760	93.392	227	15	0	1	227	1	227	1.89e-154	427
MS.gene56241.t1	MTR_4g035855	42.073	164	90	2	66	227	43	203	1.98e-35	124
MS.gene56241.t1	MTR_3g008840	39.181	171	97	3	55	224	18	182	4.26e-32	115
MS.gene56241.t1	MTR_8g104765	34.595	185	112	2	48	224	26	209	1.30e-31	115
MS.gene56241.t1	MTR_4g120825	33.871	186	114	2	47	224	16	200	3.15e-31	114
MS.gene56241.t1	MTR_4g120830	33.871	186	114	2	47	224	16	200	4.16e-31	114
MS.gene56241.t1	MTR_4g035905	35.754	179	108	3	45	223	19	190	4.58e-31	113
MS.gene56241.t1	MTR_3g008820	38.125	160	96	2	66	224	24	181	1.57e-30	111
MS.gene56241.t1	MTR_4g035880	36.709	158	97	2	66	223	36	190	3.17e-30	111
MS.gene56241.t1	MTR_4g035870	36.709	158	97	2	66	223	36	190	8.74e-30	110
MS.gene56241.t1	MTR_5g012000	33.540	161	103	2	66	224	48	206	3.95e-26	101
MS.gene56241.t1	MTR_4g101760	32.317	164	101	3	66	224	39	197	8.68e-22	89.4
MS.gene56241.t1	MTR_2g043560	32.967	182	98	7	50	224	21	185	6.55e-20	84.3
MS.gene56241.t1	MTR_4g124855	27.746	173	120	3	54	223	24	194	1.30e-18	81.3
MS.gene56241.t1	MTR_4g025550	31.351	185	105	7	47	219	17	191	2.95e-16	74.7
MS.gene56241.t1	MTR_4g025590	31.737	167	96	6	65	219	2	162	3.13e-16	74.3
MS.gene56241.t1	MTR_4g025610	31.928	166	97	6	65	219	31	191	1.30e-15	73.2
MS.gene56241.t1	MTR_7g108790	27.072	181	117	5	45	223	18	185	2.17e-15	72.4
MS.gene56241.t1	MTR_4g025620	29.670	182	106	7	50	219	20	191	5.56e-14	68.6

Query id	Subject id	identity %	alignment length	mismatches	gap openings	q. start	q. end	s. start	s. end	e-value	bit score
MS.gene56241.t1	AT5G51520	38.265	196	104	3	40	227	18	204	2.87e-40	137
MS.gene56241.t1	AT4G25250	35.359	181	113	3	45	224	18	195	5.77e-36	126
MS.gene56241.t1	AT2G01610	34.615	182	103	4	56	223	35	214	6.50e-33	119
MS.gene56241.t1	AT4G25260	37.037	162	97	2	66	224	37	196	1.95e-32	117
MS.gene56241.t1	AT1G62760	43.353	173	84	6	55	223	140	302	3.40e-32	119
MS.gene56241.t1	AT5G62360	36.478	159	97	2	66	224	43	197	3.95e-30	111
MS.gene56241.t1	AT5G62350	34.161	161	101	2	66	223	38	196	9.15e-30	110
MS.gene56241.t1	AT1G14890	34.483	174	102	3	54	223	47	212	1.08e-29	110
MS.gene56241.t1	AT3G47380	33.898	177	112	2	50	223	22	196	1.30e-29	109
MS.gene56241.t1	AT1G70720	34.483	174	98	4	54	223	30	191	3.78e-26	100
MS.gene56241.t1	AT1G70720	34.483	174	98	4	54	223	42	203	3.85e-26	101
MS.gene56241.t1	AT1G62770	32.759	174	112	3	55	224	22	194	4.55e-25	98.2
MS.gene56241.t1	AT1G23205	34.320	169	102	3	66	226	33	200	1.25e-24	97.1
MS.gene56241.t1	AT5G20740	29.762	168	111	4	61	224	28	192	5.33e-22	90.1
MS.gene56241.t1	AT5G20740	29.762	168	111	4	61	224	62	226	6.53e-22	90.5
MS.gene56241.t1	AT4G12390	34.356	163	101	3	66	224	39	199	1.35e-19	83.6
MS.gene56241.t1	AT4G00080	32.044	181	110	5	53	224	22	198	1.07e-18	81.3
MS.gene56241.t1	AT3G62820	31.737	167	99	5	64	224	31	188	2.06e-18	80.5
MS.gene56241.t1	AT2G47670	32.558	172	100	6	59	222	37	200	5.74e-17	76.6
MS.gene56241.t1	AT1G53830	25.316	158	117	1	67	224	62	218	2.95e-11	62.8

Find 29 sgRNAs with CRISPR-Local

Find 42 sgRNAs with CRISPR-GE

CRISPR-Local

CRISPR-Local
sgRNA_sequence	on_target_score	Position	Region
TCTTGATGCCATGGCACATT+TGG	0.327434	3.3:+4395927	MS.gene56241:CDS
AAAGATTTGTAACATATATA+TGG	0.334992	3.3:-4395697	None:intergenic
CTTGTTTAGAAGCTTTGATA+TGG	0.395752	3.3:-4395815	None:intergenic
AATGATCAAACTTGCTATGA+TGG	0.416303	3.3:+4396015	MS.gene56241:CDS
ATATGAAGTGTATGAAAAGT+TGG	0.423985	3.3:-4395602	None:intergenic
GACAATAAATTGTCAAGCAT+TGG	0.458680	3.3:+4395841	MS.gene56241:CDS
AGTGTATGAAAAGTTGGTTT+TGG	0.460748	3.3:-4395596	None:intergenic
TTTGAAATCGTTGGTGTTGG+TGG	0.482076	3.3:-4395638	None:intergenic
GAAGTTGTGCAAGATTGCTT+TGG	0.484978	3.3:+4395868	MS.gene56241:CDS
TCTAATCTTGTTCCCAAGTG+TGG	0.490488	3.3:-4396061	None:intergenic
ATGAATGTGGATTCCACACT+TGG	0.495060	3.3:+4396048	MS.gene56241:CDS
GTTGGTGTTGGTGGAATTTG+AGG	0.503854	3.3:-4395629	None:intergenic
AGATTAGTAACATAAAGACT+TGG	0.517077	3.3:+4395974	MS.gene56241:CDS
GCAAGATTCTCTTGATGCCA+TGG	0.535597	3.3:+4395918	MS.gene56241:CDS
TTGGTGTTGGTGGAATTTGA+GGG	0.537329	3.3:-4395628	None:intergenic
GCAAGTTTGATCATTAGTAA+TGG	0.544323	3.3:-4396007	None:intergenic
ATGATCAAACTTGCTATGAT+GGG	0.546835	3.3:+4396016	MS.gene56241:CDS
GTAACATATATATGGATATG+TGG	0.547384	3.3:-4395689	None:intergenic
GATTAGTAACATAAAGACTT+GGG	0.551030	3.3:+4395975	MS.gene56241:CDS
CATATCAAAGCTTCTAAACA+AGG	0.561127	3.3:+4395816	MS.gene56241:CDS
ATTATGCAACACTTCTCTCT+CGG	0.582617	3.3:+4395759	MS.gene56241:CDS
TAGAAGCTTTGATATGGCGG+AGG	0.586848	3.3:-4395809	None:intergenic
GTTTAGAAGCTTTGATATGG+CGG	0.588835	3.3:-4395812	None:intergenic
ATCAGCATCCAAATGTGCCA+TGG	0.600401	3.3:-4395935	None:intergenic
TAGCATCACTTCAGCATGTG+AGG	0.601096	3.3:+4395546	MS.gene56241:CDS
AGATTGCTTTGGCAATGTGA+AGG	0.623852	3.3:+4395879	MS.gene56241:CDS
TGGGTTTGATGATATGAATG+TGG	0.629419	3.3:+4396035	MS.gene56241:CDS
TGAATGTGGATTCCACACTT+GGG	0.667297	3.3:+4396049	MS.gene56241:CDS
TCTCTCGGACGCATTAAATG+CGG	0.679364	3.3:+4395774	MS.gene56241:CDS

CRISPR-GE

badsite warning	sgRNA_sequence	Strand	Position	Region	GC_content
!!	AAAGATTTGTAACATATATA+TGG	-	chr3.3:4395700-4395719	None:intergenic	15.0%
!!!	ATTGCATGAATTTTTAATAT+AGG	-	chr3.3:4395665-4395684	None:intergenic	15.0%
!	AGATTAGTAACATAAAGACT+TGG	+	chr3.3:4395974-4395993	MS.gene56241:CDS	25.0%
!	ATATGAAGTGTATGAAAAGT+TGG	-	chr3.3:4395605-4395624	None:intergenic	25.0%
!	GATTAGTAACATAAAGACTT+GGG	+	chr3.3:4395975-4395994	MS.gene56241:CDS	25.0%
!	GTAACATATATATGGATATG+TGG	-	chr3.3:4395692-4395711	None:intergenic	25.0%
!!	AGAAAAACTTGAAAAAGTGT+TGG	-	chr3.3:4395479-4395498	None:intergenic	25.0%
!!!	AATATAGGTTTTGAAATCGT+TGG	-	chr3.3:4395650-4395669	None:intergenic	25.0%
	CATATCAAAGCTTCTAAACA+AGG	+	chr3.3:4395816-4395835	MS.gene56241:CDS	30.0%
	GACAATAAATTGTCAAGCAT+TGG	+	chr3.3:4395841-4395860	MS.gene56241:CDS	30.0%
	GCAAGTTTGATCATTAGTAA+TGG	-	chr3.3:4396010-4396029	None:intergenic	30.0%
	TCCATATGCTTCAAAAATTC+AGG	+	chr3.3:4395723-4395742	MS.gene56241:CDS	30.0%
!	CTTGTTTAGAAGCTTTGATA+TGG	-	chr3.3:4395818-4395837	None:intergenic	30.0%
!!	AATGATCAAACTTGCTATGA+TGG	+	chr3.3:4396015-4396034	MS.gene56241:CDS	30.0%
!!	AGTGTATGAAAAGTTGGTTT+TGG	-	chr3.3:4395599-4395618	None:intergenic	30.0%
!!	ATGATCAAACTTGCTATGAT+GGG	+	chr3.3:4396016-4396035	MS.gene56241:CDS	30.0%
!!!	GAAGTGTTGCATAATTTTAG+AGG	-	chr3.3:4395754-4395773	None:intergenic	30.0%
!!!	TAGAAGAAGAGTTTTGAATG+TGG	+	chr3.3:4396080-4396099	MS.gene56241:CDS	30.0%
	ATTATGCAACACTTCTCTCT+CGG	+	chr3.3:4395759-4395778	MS.gene56241:CDS	35.0%
!	GTTTAGAAGCTTTGATATGG+CGG	-	chr3.3:4395815-4395834	None:intergenic	35.0%
!	TGGGTTTGATGATATGAATG+TGG	+	chr3.3:4396035-4396054	MS.gene56241:CDS	35.0%
!!!	AAAGTTGGTTTTGGTGGAAT+TGG	-	chr3.3:4395590-4395609	None:intergenic	35.0%
!!!	GCCTGAATTTTTGAAGCATA+TGG	-	chr3.3:4395727-4395746	None:intergenic	35.0%
!!!	GTATGAAAAGTTGGTTTTGG+TGG	-	chr3.3:4395596-4395615	None:intergenic	35.0%
	AGATTGCTTTGGCAATGTGA+AGG	+	chr3.3:4395879-4395898	MS.gene56241:CDS	40.0%
	ATGAATGTGGATTCCACACT+TGG	+	chr3.3:4396048-4396067	MS.gene56241:CDS	40.0%
	GAAGTTGTGCAAGATTGCTT+TGG	+	chr3.3:4395868-4395887	MS.gene56241:CDS	40.0%
	TCTAATCTTGTTCCCAAGTG+TGG	-	chr3.3:4396064-4396083	None:intergenic	40.0%
	TGAATGTGGATTCCACACTT+GGG	+	chr3.3:4396049-4396068	MS.gene56241:CDS	40.0%
!!	TTGGTGTTGGTGGAATTTGA+GGG	-	chr3.3:4395631-4395650	None:intergenic	40.0%
!!	TTTGAAATCGTTGGTGTTGG+TGG	-	chr3.3:4395641-4395660	None:intergenic	40.0%
!!!	GAAGAGTTTTGAATGTGGCA+AGG	+	chr3.3:4396085-4396104	MS.gene56241:CDS	40.0%
!!!	GGTTTTGAAATCGTTGGTGT+TGG	-	chr3.3:4395644-4395663	None:intergenic	40.0%
!!!	TGGTTTTGGTGGAATTGGTA+GGG	-	chr3.3:4395585-4395604	None:intergenic	40.0%
!!!	TTGGTTTTGGTGGAATTGGT+AGG	-	chr3.3:4395586-4395605	None:intergenic	40.0%
	ATCAGCATCCAAATGTGCCA+TGG	-	chr3.3:4395938-4395957	None:intergenic	45.0%
	GCAAGATTCTCTTGATGCCA+TGG	+	chr3.3:4395918-4395937	MS.gene56241:CDS	45.0%
	TAGCATCACTTCAGCATGTG+AGG	+	chr3.3:4395546-4395565	MS.gene56241:CDS	45.0%
	TCTCTCGGACGCATTAAATG+CGG	+	chr3.3:4395774-4395793	MS.gene56241:CDS	45.0%
	TCTTGATGCCATGGCACATT+TGG	+	chr3.3:4395927-4395946	MS.gene56241:CDS	45.0%
!	TAGAAGCTTTGATATGGCGG+AGG	-	chr3.3:4395812-4395831	None:intergenic	45.0%
!!	GTTGGTGTTGGTGGAATTTG+AGG	-	chr3.3:4395632-4395651	None:intergenic	45.0%

Chromosome	Type	Strat	End	Strand	Name
chr3.3	gene	4395469	4396152	4395469	ID=MS.gene56241
chr3.3	mRNA	4395469	4396152	4395469	ID=MS.gene56241.t1;Parent=MS.gene56241
chr3.3	exon	4395469	4396152	4395469	ID=MS.gene56241.t1.exon1;Parent=MS.gene56241.t1
chr3.3	CDS	4395469	4396152	4395469	ID=cds.MS.gene56241.t1;Parent=MS.gene56241.t1

Gene Sequence

>MS.gene56241

ATGGAAGCCAACACTTTTTCAAGTTTTTCTTTCAGTCATGTTCTCAAATTTGCAATTTTGATATTGCTAGCTATATCTAGCATCACTTCAGCATGTGAGGACAATTCTACAATCCCTACCAATTCCACCAAAACCAACTTTTCATACACTTCATATGCTCCCTCAAATTCCACCAACACCAACGATTTCAAAACCTATATTAAAAATTCATGCAATTCAACCACATATCCATATATATGTTACAAATCTTTATCTCCATATGCTTCAAAAATTCAGGCAGATCCTCTAAAATTATGCAACACTTCTCTCTCGGACGCATTAAATGCGGCTCGTGATGCATCCTCCGCCATATCAAAGCTTCTAAACAAGGATGACAATAAATTGTCAAGCATTGGTGAAGAAGTTGTGCAAGATTGCTTTGGCAATGTGAAGGATTCAATAGAGCAGTTGCAAGATTCTCTTGATGCCATGGCACATTTGGATGCTGATTTTGATAGAGAATTTCAGATTAGTAACATAAAGACTTGGGTTAGTTCAGCCATTACTAATGATCAAACTTGCTATGATGGGTTTGATGATATGAATGTGGATTCCACACTTGGGAACAAGATTAGAAGAAGAGTTTTGAATGTGGCAAGGAAGACTAGCAATGCTTTGTATTTTATCAACAATAACATATATTAA

Protein sequence

>MS.gene56241.t1

MEANTFSSFSFSHVLKFAILILLAISSITSACEDNSTIPTNSTKTNFSYTSYAPSNSTNTNDFKTYIKNSCNSTTYPYICYKSLSPYASKIQADPLKLCNTSLSDALNAARDASSAISKLLNKDDNKLSSIGEEVVQDCFGNVKDSIEQLQDSLDAMAHLDADFDREFQISNIKTWVSSAITNDQTCYDGFDDMNVDSTLGNKIRRRVLNVARKTSNALYFINNNIY