AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene21143


Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21143.t1 MTR_5g012000 97.449 196 5 0 1 196 22 217 1.74e-142 395
MS.gene21143.t1 MTR_4g101760 63.402 194 60 2 9 191 10 203 9.12e-85 249
MS.gene21143.t1 MTR_3g008840 53.571 168 77 1 23 190 21 187 8.04e-65 198
MS.gene21143.t1 MTR_3g008820 51.190 168 81 1 23 190 20 186 3.72e-62 191
MS.gene21143.t1 MTR_4g035905 43.030 165 91 1 27 191 36 197 6.51e-45 147
MS.gene21143.t1 MTR_4g035870 38.191 199 111 2 1 190 1 196 6.50e-42 140
MS.gene21143.t1 MTR_4g035880 42.073 164 92 1 27 190 36 196 2.55e-41 138
MS.gene21143.t1 MTR_4g025550 38.308 201 106 6 9 195 10 206 8.50e-39 132
MS.gene21143.t1 MTR_4g025610 37.245 196 115 4 7 195 12 206 7.60e-38 130
MS.gene21143.t1 MTR_4g025590 38.636 176 96 4 27 193 3 175 1.37e-36 126
MS.gene21143.t1 MTR_4g025620 35.567 194 113 4 7 191 12 202 1.23e-35 124
MS.gene21143.t1 MTR_8g104765 38.596 171 96 3 25 187 42 211 1.70e-33 119
MS.gene21143.t1 MTR_4g120825 38.596 171 96 3 25 187 33 202 1.71e-33 119
MS.gene21143.t1 MTR_4g120830 38.596 171 96 3 25 187 33 202 2.69e-33 118
MS.gene21143.t1 MTR_7g108790 35.802 162 99 2 28 189 34 190 3.26e-30 110
MS.gene21143.t1 MTR_3g008760 34.161 161 102 2 27 185 66 224 5.41e-28 105
MS.gene21143.t1 MTR_2g043560 35.912 181 95 6 16 189 23 189 1.26e-25 98.6
MS.gene21143.t1 MTR_4g124855 31.606 193 115 5 8 189 13 199 2.45e-24 95.5
MS.gene21143.t1 MTR_4g035855 31.056 161 110 1 27 187 43 202 1.24e-22 90.9
MS.gene21143.t1 MTR_1g088540 30.405 148 98 2 42 189 1 143 3.99e-19 80.1
MS.gene21143.t1 MTR_3g091380 30.864 162 107 2 24 182 74 233 1.20e-17 80.5
MS.gene21143.t1 MTR_1g008140 29.762 168 106 3 24 182 78 242 9.21e-17 78.2
MS.gene21143.t1 MTR_8g023310 25.478 157 113 2 28 182 68 222 8.30e-15 72.4
MS.gene21143.t1 MTR_8g023150 29.341 167 108 5 20 183 69 228 3.83e-11 61.6
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene21143.t1 AT1G62770 50.244 205 92 6 1 196 1 204 5.06e-61 189
MS.gene21143.t1 AT4G12390 56.805 169 69 2 27 192 39 206 1.79e-60 187
MS.gene21143.t1 AT4G25260 46.409 181 96 1 11 190 21 201 2.27e-55 174
MS.gene21143.t1 AT5G62350 47.273 165 86 1 27 190 38 202 8.20e-55 173
MS.gene21143.t1 AT3G47380 42.784 194 101 2 7 190 9 202 3.56e-52 166
MS.gene21143.t1 AT5G62360 41.818 165 92 2 26 190 42 202 2.57e-45 149
MS.gene21143.t1 AT1G62760 43.182 176 90 2 20 191 140 309 7.70e-42 143
MS.gene21143.t1 AT1G14890 41.566 166 90 2 26 189 57 217 8.00e-39 133
MS.gene21143.t1 AT2G01610 40.113 177 93 3 26 189 43 219 2.79e-37 129
MS.gene21143.t1 AT5G20740 40.361 166 90 3 26 186 32 193 1.61e-35 124
MS.gene21143.t1 AT5G20740 40.361 166 90 3 26 186 66 227 4.95e-35 124
MS.gene21143.t1 AT4G25250 34.969 163 104 2 27 188 37 198 2.48e-34 120
MS.gene21143.t1 AT4G00080 36.683 199 111 6 1 185 1 198 2.75e-34 121
MS.gene21143.t1 AT1G70720 34.254 181 110 3 12 187 18 194 2.86e-29 107
MS.gene21143.t1 AT1G70720 34.254 181 110 3 12 187 30 206 3.38e-29 108
MS.gene21143.t1 AT5G51520 33.929 168 101 2 27 186 37 202 8.61e-28 104
MS.gene21143.t1 AT1G23205 35.870 184 105 4 11 184 17 197 2.71e-25 97.8
MS.gene21143.t1 AT3G62820 32.796 186 115 5 8 188 11 191 1.76e-22 90.1
MS.gene21143.t1 AT3G14310 27.933 179 124 3 14 189 43 219 8.97e-21 89.7
MS.gene21143.t1 AT2G47670 30.675 163 109 2 25 187 46 204 1.03e-19 83.2
MS.gene21143.t1 AT1G53830 28.235 170 119 2 21 189 55 222 8.07e-18 81.3
MS.gene21143.t1 AT3G49220 32.184 174 92 7 24 190 128 282 2.24e-15 73.9
MS.gene21143.t1 AT3G49220 32.184 174 92 7 24 190 128 282 3.39e-15 73.6
MS.gene21143.t1 AT3G49220 32.184 174 92 7 24 190 83 237 4.03e-15 73.2
MS.gene21143.t1 AT5G53370 27.500 160 108 4 24 182 107 259 4.39e-13 67.4
MS.gene21143.t1 AT5G53370 27.500 160 108 4 24 182 71 223 5.08e-13 67.0
MS.gene21143.t1 AT3G14300 26.136 176 123 2 22 191 265 439 2.25e-12 65.5
MS.gene21143.t1 AT5G62340 25.616 203 120 7 9 190 14 206 2.77e-11 60.5

Find 53 sgRNAs with CRISPR-Local

Find 51 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GATGATAACACTTGTCTTGA+TGG 0.271370 5.1:+5417893 MS.gene21143:CDS
ACTTGTCTTGATGGCTTTGC+TGG 0.284433 5.1:+5417902 MS.gene21143:CDS
TAGAGCAGGGGTTTATTTGC+TGG 0.342763 5.1:+5417472 MS.gene21143:CDS
AGAAAACATGGGTGACAGTT+TGG 0.352972 5.1:+5417760 MS.gene21143:CDS
ATTGACAACCCTGTCCTTGA+TGG 0.385514 5.1:-5417954 None:intergenic
ACAGTCTTAGCCAATCGGTT+AGG 0.388918 5.1:+5417783 MS.gene21143:CDS
TACCAGCAACACACTCGCTT+TGG 0.400248 5.1:+5417988 MS.gene21143:CDS
ATGGGGTATGCGAGCGTGAT+TGG 0.403801 5.1:+5417599 MS.gene21143:CDS
AACCAAAGCGAGTGTGTTGC+TGG 0.408904 5.1:-5417990 None:intergenic
TTCCATGAATGGAATTGTTA+AGG 0.412818 5.1:+5417928 MS.gene21143:CDS
TCGTTAGTTGTCGCTCGCTT+TGG 0.415738 5.1:-5417622 None:intergenic
AGCCTTAACAATTCCATTCA+TGG 0.416556 5.1:-5417930 None:intergenic
GTAAACATGGCGGCTAGAGC+AGG 0.417002 5.1:+5417458 None:intergenic
ACATGAACAACGTGCAGACT+TGG 0.438369 5.1:+5417852 MS.gene21143:CDS
AGCCAATCGGTTAGGGAGTT+AGG 0.439103 5.1:+5417791 MS.gene21143:CDS
TTTGCTGGTCCTTCCATGAA+TGG 0.446590 5.1:+5417917 MS.gene21143:CDS
CGATGCACTCGATCGTGTCC+TGG 0.450154 5.1:-5417669 None:intergenic
CAGTCTTAGCCAATCGGTTA+GGG 0.452763 5.1:+5417784 MS.gene21143:CDS
AGACATTGAACGCATAAAAC+AGG 0.460054 5.1:-5417575 None:intergenic
GGCAGTATTGGTCATGCTGT+TGG 0.460878 5.1:+5417812 MS.gene21143:CDS
TTTATGCGTTCAATGTCTCA+TGG 0.462904 5.1:+5417580 MS.gene21143:CDS
GTTAGGGAGTTAGGCAGTAT+TGG 0.470731 5.1:+5417800 MS.gene21143:CDS
GAACTCTGATGGGGTTATAG+AGG 0.476311 5.1:-5417522 None:intergenic
TTAAGGCTGCCATCAAGGAC+AGG 0.476862 5.1:+5417945 MS.gene21143:CDS
TGTACGGCTCTGTACTCCCT+TGG 0.486585 5.1:-5417728 None:intergenic
CTCTATCAGTGAGCATATCC+AGG 0.495431 5.1:+5417651 MS.gene21143:CDS
TTATGCGTTCAATGTCTCAT+GGG 0.496994 5.1:+5417581 MS.gene21143:CDS
TTTGGACAGTCTTAGCCAAT+CGG 0.504656 5.1:+5417778 MS.gene21143:CDS
GGCAAGAGGCATCAAGCCAA+GGG 0.526611 5.1:+5417712 MS.gene21143:CDS
TTCTGCAGTTCGATGGCTTG+AGG 0.534233 5.1:-5418023 None:intergenic
AATTGTTAAGGCTGCCATCA+AGG 0.538854 5.1:+5417940 MS.gene21143:CDS
TGCCTAACTCCCTAACCGAT+TGG 0.544285 5.1:-5417793 None:intergenic
AGGCAAGAGGCATCAAGCCA+AGG 0.545433 5.1:+5417711 MS.gene21143:CDS
TAAACATGGCGGCTAGAGCA+GGG 0.549343 5.1:+5417459 None:intergenic
GAGGACTTGATGAACTCTGA+TGG 0.549989 5.1:-5417533 None:intergenic
AGGACTTGATGAACTCTGAT+GGG 0.550827 5.1:-5417532 None:intergenic
AAGGAGTTTCTGCAGTTCGA+TGG 0.560457 5.1:-5418030 None:intergenic
CTGCAGTCACAAGGCTCATG+AGG 0.571475 5.1:-5417496 None:intergenic
ACAAGACTGCATAGAAAACA+TGG 0.577763 5.1:+5417748 MS.gene21143:CDS
CATGAACAACGTGCAGACTT+GGG 0.585314 5.1:+5417853 MS.gene21143:CDS
TATGCGTTCAATGTCTCATG+GGG 0.593117 5.1:+5417582 MS.gene21143:CDS
TTAACAATTCCATTCATGGA+AGG 0.596367 5.1:-5417926 None:intergenic
TAAGGCTGCCATCAAGGACA+GGG 0.619720 5.1:+5417946 MS.gene21143:CDS
TATTGGTCATGCTGTTGGAG+AGG 0.620267 5.1:+5417817 MS.gene21143:CDS
TAGAGGATTCTGCAGTCACA+AGG 0.636790 5.1:-5417505 None:intergenic
CAGAAGATATCAAAGGCAAG+AGG 0.641738 5.1:+5417698 MS.gene21143:CDS
GTTTGTACAGAAGATATCAA+AGG 0.649432 5.1:+5417691 MS.gene21143:CDS
AAACATGGCGGCTAGAGCAG+GGG 0.656730 5.1:+5417460 None:intergenic
ATAGCGAGTAGCTCTACATG+AGG 0.683240 5.1:-5417552 None:intergenic
GGATATGCTCACTGATAGAG+CGG 0.692375 5.1:-5417648 None:intergenic
GGACTTGATGAACTCTGATG+GGG 0.708124 5.1:-5417531 None:intergenic
CAAGACTGCATAGAAAACAT+GGG 0.713583 5.1:+5417749 MS.gene21143:CDS
CTGTTGGAGAGGACTTCGTG+TGG 0.729717 5.1:+5417828 MS.gene21143:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
GTTTGTACAGAAGATATCAA+AGG + chr5.1:5417691-5417710 MS.gene21143:CDS 30.0%
TTAACAATTCCATTCATGGA+AGG - chr5.1:5417929-5417948 None:intergenic 30.0%
TTCCATGAATGGAATTGTTA+AGG + chr5.1:5417928-5417947 MS.gene21143:CDS 30.0%
ACAAGACTGCATAGAAAACA+TGG + chr5.1:5417748-5417767 MS.gene21143:CDS 35.0%
AGACATTGAACGCATAAAAC+AGG - chr5.1:5417578-5417597 None:intergenic 35.0%
AGCCTTAACAATTCCATTCA+TGG - chr5.1:5417933-5417952 None:intergenic 35.0%
CAAGACTGCATAGAAAACAT+GGG + chr5.1:5417749-5417768 MS.gene21143:CDS 35.0%
GATGATAACACTTGTCTTGA+TGG + chr5.1:5417893-5417912 MS.gene21143:CDS 35.0%
TTATGCGTTCAATGTCTCAT+GGG + chr5.1:5417581-5417600 MS.gene21143:CDS 35.0%
TTTATGCGTTCAATGTCTCA+TGG + chr5.1:5417580-5417599 MS.gene21143:CDS 35.0%
!! GTTTTCTATGCAGTCTTGTA+CGG - chr5.1:5417747-5417766 None:intergenic 35.0%
AATTGTTAAGGCTGCCATCA+AGG + chr5.1:5417940-5417959 MS.gene21143:CDS 40.0%
AGAAAACATGGGTGACAGTT+TGG + chr5.1:5417760-5417779 MS.gene21143:CDS 40.0%
AGGACTTGATGAACTCTGAT+GGG - chr5.1:5417535-5417554 None:intergenic 40.0%
CAGAAGATATCAAAGGCAAG+AGG + chr5.1:5417698-5417717 MS.gene21143:CDS 40.0%
TATGCGTTCAATGTCTCATG+GGG + chr5.1:5417582-5417601 MS.gene21143:CDS 40.0%
TTTGGACAGTCTTAGCCAAT+CGG + chr5.1:5417778-5417797 MS.gene21143:CDS 40.0%
AAGGAGTTTCTGCAGTTCGA+TGG - chr5.1:5418033-5418052 None:intergenic 45.0%
ACAGTCTTAGCCAATCGGTT+AGG + chr5.1:5417783-5417802 MS.gene21143:CDS 45.0%
ACATGAACAACGTGCAGACT+TGG + chr5.1:5417852-5417871 MS.gene21143:CDS 45.0%
ATTGACAACCCTGTCCTTGA+TGG - chr5.1:5417957-5417976 None:intergenic 45.0%
CAGTCTTAGCCAATCGGTTA+GGG + chr5.1:5417784-5417803 MS.gene21143:CDS 45.0%
CATGAACAACGTGCAGACTT+GGG + chr5.1:5417853-5417872 MS.gene21143:CDS 45.0%
CTCTATCAGTGAGCATATCC+AGG + chr5.1:5417651-5417670 MS.gene21143:CDS 45.0%
GAACTCTGATGGGGTTATAG+AGG - chr5.1:5417525-5417544 None:intergenic 45.0%
GAGGACTTGATGAACTCTGA+TGG - chr5.1:5417536-5417555 None:intergenic 45.0%
GGACTTGATGAACTCTGATG+GGG - chr5.1:5417534-5417553 None:intergenic 45.0%
GGATATGCTCACTGATAGAG+CGG - chr5.1:5417651-5417670 None:intergenic 45.0%
GTTAGGGAGTTAGGCAGTAT+TGG + chr5.1:5417800-5417819 MS.gene21143:CDS 45.0%
TAGAGCAGGGGTTTATTTGC+TGG + chr5.1:5417472-5417491 MS.gene21143:CDS 45.0%
TAGAGGATTCTGCAGTCACA+AGG - chr5.1:5417508-5417527 None:intergenic 45.0%
TATTGGTCATGCTGTTGGAG+AGG + chr5.1:5417817-5417836 MS.gene21143:CDS 45.0%
TTTGCTGGTCCTTCCATGAA+TGG + chr5.1:5417917-5417936 MS.gene21143:CDS 45.0%
! ACTTGTCTTGATGGCTTTGC+TGG + chr5.1:5417902-5417921 MS.gene21143:CDS 45.0%
! ATAGCGAGTAGCTCTACATG+AGG - chr5.1:5417555-5417574 None:intergenic 45.0%
AACCAAAGCGAGTGTGTTGC+TGG - chr5.1:5417993-5418012 None:intergenic 50.0%
AGCCAATCGGTTAGGGAGTT+AGG + chr5.1:5417791-5417810 MS.gene21143:CDS 50.0%
TAAGGCTGCCATCAAGGACA+GGG + chr5.1:5417946-5417965 MS.gene21143:CDS 50.0%
TACCAGCAACACACTCGCTT+TGG + chr5.1:5417988-5418007 MS.gene21143:CDS 50.0%
TCGTTAGTTGTCGCTCGCTT+TGG - chr5.1:5417625-5417644 None:intergenic 50.0%
TGCCTAACTCCCTAACCGAT+TGG - chr5.1:5417796-5417815 None:intergenic 50.0%
TTAAGGCTGCCATCAAGGAC+AGG + chr5.1:5417945-5417964 MS.gene21143:CDS 50.0%
TTCTGCAGTTCGATGGCTTG+AGG - chr5.1:5418026-5418045 None:intergenic 50.0%
!! GGCAGTATTGGTCATGCTGT+TGG + chr5.1:5417812-5417831 MS.gene21143:CDS 50.0%
ATGGGGTATGCGAGCGTGAT+TGG + chr5.1:5417599-5417618 MS.gene21143:CDS 55.0%
CTGCAGTCACAAGGCTCATG+AGG - chr5.1:5417499-5417518 None:intergenic 55.0%
CTGTTGGAGAGGACTTCGTG+TGG + chr5.1:5417828-5417847 MS.gene21143:CDS 55.0%
TGTACGGCTCTGTACTCCCT+TGG - chr5.1:5417731-5417750 None:intergenic 55.0%
!! AGGCAAGAGGCATCAAGCCA+AGG + chr5.1:5417711-5417730 MS.gene21143:CDS 55.0%
!! GGCAAGAGGCATCAAGCCAA+GGG + chr5.1:5417712-5417731 MS.gene21143:CDS 55.0%
CGATGCACTCGATCGTGTCC+TGG - chr5.1:5417672-5417691 None:intergenic 60.0%


Chromosome Type Strat End Strand Name
chr5.1 gene 5417464 5418054 5417464 ID=MS.gene21143
chr5.1 mRNA 5417464 5418054 5417464 ID=MS.gene21143.t1;Parent=MS.gene21143
chr5.1 exon 5417464 5418054 5417464 ID=MS.gene21143.t1.exon1;Parent=MS.gene21143.t1
chr5.1 CDS 5417464 5418054 5417464 ID=cds.MS.gene21143.t1;Parent=MS.gene21143.t1
Gene Sequence

>MS.gene21143

ATGGCGGCTAGAGCAGGGGTTTATTTGCTGGTCCTCATGAGCCTTGTGACTGCAGAATCCTCTATAACCCCATCAGAGTTCATCAAGTCCTCATGTAGAGCTACTCGCTATCCTGTTTTATGCGTTCAATGTCTCATGGGGTATGCGAGCGTGATTGGCCAAAGCGAGCGACAACTAACGATAACCGCTCTATCAGTGAGCATATCCAGGACACGATCGAGTGCATCGTTTGTACAGAAGATATCAAAGGCAAGAGGCATCAAGCCAAGGGAGTACAGAGCCGTACAAGACTGCATAGAAAACATGGGTGACAGTTTGGACAGTCTTAGCCAATCGGTTAGGGAGTTAGGCAGTATTGGTCATGCTGTTGGAGAGGACTTCGTGTGGCACATGAACAACGTGCAGACTTGGGTCAGTGCTGCACTTACTGATGATAACACTTGTCTTGATGGCTTTGCTGGTCCTTCCATGAATGGAATTGTTAAGGCTGCCATCAAGGACAGGGTTGTCAATGTTGCTCAAGTTACCAGCAACACACTCGCTTTGGTTAATCACTTCGCCTCAAGCCATCGAACTGCAGAAACTCCTTAA

Protein sequence

>MS.gene21143.t1

MAARAGVYLLVLMSLVTAESSITPSEFIKSSCRATRYPVLCVQCLMGYASVIGQSERQLTITALSVSISRTRSSASFVQKISKARGIKPREYRAVQDCIENMGDSLDSLSQSVRELGSIGHAVGEDFVWHMNNVQTWVSAALTDDNTCLDGFAGPSMNGIVKAAIKDRVVNVAQVTSNTLALVNHFASSHRTAETP