Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56261.t1 | XP_013458821.1 | 100 | 318 | 0 | 0 | 1 | 318 | 1 | 318 | 1.80E-153 | 552 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56261.t1 | Q8W206 | 73.1 | 320 | 81 | 2 | 1 | 318 | 1 | 317 | 2.8e-126 | 453.0 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56261.t1 | I3T146 | 100.0 | 318 | 0 | 0 | 1 | 318 | 1 | 318 | 1.3e-153 | 552.0 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene51629 | MS.gene56261 | PPI |
| MS.gene37341 | MS.gene56261 | PPI |
| MS.gene011205 | MS.gene56261 | PPI |
| MS.gene07775 | MS.gene56261 | PPI |
| MS.gene63516 | MS.gene56261 | PPI |
| MS.gene56261 | MS.gene009461 | PPI |
| MS.gene009461 | MS.gene56261 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56261.t1 | MTR_3g009270 | 100.000 | 318 | 0 | 0 | 1 | 318 | 1 | 318 | 0.0 | 659 |
| MS.gene56261.t1 | MTR_5g081980 | 26.014 | 296 | 181 | 6 | 13 | 299 | 19 | 285 | 2.64e-23 | 98.2 |
| MS.gene56261.t1 | MTR_3g070180 | 25.574 | 305 | 189 | 6 | 4 | 299 | 10 | 285 | 9.82e-23 | 96.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56261.t1 | AT5G56280 | 81.250 | 320 | 55 | 2 | 1 | 318 | 1 | 317 | 0.0 | 539 |
| MS.gene56261.t1 | AT4G26430 | 78.056 | 319 | 67 | 2 | 1 | 318 | 1 | 317 | 0.0 | 522 |
| MS.gene56261.t1 | AT5G05780 | 24.915 | 293 | 185 | 5 | 13 | 299 | 19 | 282 | 2.95e-23 | 97.8 |
| MS.gene56261.t1 | AT5G05780 | 25.000 | 296 | 184 | 6 | 13 | 299 | 19 | 285 | 4.81e-23 | 97.4 |
| MS.gene56261.t1 | AT3G11270 | 24.915 | 293 | 185 | 5 | 13 | 299 | 19 | 282 | 3.83e-22 | 94.7 |
| MS.gene56261.t1 | AT3G11270 | 25.000 | 296 | 184 | 6 | 13 | 299 | 19 | 285 | 8.40e-22 | 94.0 |
Find 72 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCACCGCCACGTGTCTT+TGG | 0.239123 | 3.1:-4858902 | MS.gene56261:CDS |
| TATCTTCTTGCTATGGAAAA+AGG | 0.264053 | 3.1:-4854709 | MS.gene56261:intron |
| GCAGTGGCTGCTAACCTTAC+TGG | 0.270853 | 3.1:-4854784 | MS.gene56261:intron |
| TTTCCACACTTCTACATATT+GGG | 0.288492 | 3.1:-4858177 | MS.gene56261:CDS |
| TTCTGTGAGTGATTGATTGC+AGG | 0.321468 | 3.1:+4856877 | None:intergenic |
| AGCAGTTCGTCCACCCCTTC+TGG | 0.322127 | 3.1:+4851682 | None:intergenic |
| TCAGAACTGCATGTCATAGA+TGG | 0.338928 | 3.1:-4856268 | MS.gene56261:intron |
| AGATTGCCTGCTATTGAATC+TGG | 0.341800 | 3.1:-4854254 | MS.gene56261:CDS |
| CTTGTGAGAATTCACTATTA+AGG | 0.390102 | 3.1:-4854298 | MS.gene56261:CDS |
| AATGCTTCATAGCAGAATAA+AGG | 0.397301 | 3.1:-4854743 | MS.gene56261:CDS |
| CAATAGCAGGCAATCTTCTC+AGG | 0.404144 | 3.1:+4854261 | None:intergenic |
| ATTTCAAGATGATTTCTTGA+TGG | 0.415768 | 3.1:-4854228 | MS.gene56261:intron |
| GGGTTCAGAAGAACATAAAC+TGG | 0.421701 | 3.1:+4856898 | None:intergenic |
| CGTGTCTTTGGTTGTGTCAT+CGG | 0.426062 | 3.1:-4858890 | MS.gene56261:CDS |
| CGATCAAGCGAATGAGTAGA+AGG | 0.429828 | 3.1:+4858812 | None:intergenic |
| CTGTAGTGCAATGAATGATT+TGG | 0.433232 | 3.1:-4851738 | MS.gene56261:intron |
| AGAGTTCTTGCTTCTTCTCA+AGG | 0.446432 | 3.1:+4858783 | None:intergenic |
| ATATTGGGATGGTATTCAAC+TGG | 0.453349 | 3.1:-4858162 | MS.gene56261:CDS |
| ACCGTCACCGCCGTTGTTGG+TGG | 0.453826 | 3.1:+4858937 | None:intergenic |
| GGCAAGGTAACTGATCAATA+AGG | 0.459470 | 3.1:+4852963 | None:intergenic |
| CCGCTCAAGCTATACTATTG+AGG | 0.482131 | 3.1:-4856224 | MS.gene56261:intron |
| AATTTCCCAGATTCAATAGC+AGG | 0.482170 | 3.1:+4854248 | None:intergenic |
| TGGCTTCTTCATCGAGCAGC+GGG | 0.491602 | 3.1:-4859060 | MS.gene56261:CDS |
| AACACCGTCACCGCCGTTGT+TGG | 0.499135 | 3.1:+4858934 | None:intergenic |
| TTGTTGTGAGGAGCGAGAGT+AGG | 0.501655 | 3.1:+4858962 | None:intergenic |
| GCCACCAACAACGGCGGTGA+CGG | 0.509928 | 3.1:-4858938 | MS.gene56261:CDS |
| TCTTGCTTCTTCTCAAGGAA+AGG | 0.513508 | 3.1:+4858788 | None:intergenic |
| TCTGTGAGTGATTGATTGCA+GGG | 0.515077 | 3.1:+4856878 | None:intergenic |
| CCACTTTCAAATATACTGAC+GGG | 0.524749 | 3.1:+4856847 | None:intergenic |
| TGTTGTGAGGAGCGAGAGTA+GGG | 0.525975 | 3.1:+4858963 | None:intergenic |
| ACATCACTATCTTCTTGCTA+TGG | 0.532058 | 3.1:-4854716 | MS.gene56261:CDS |
| CAACCAAAGACACGTGGCGG+TGG | 0.536536 | 3.1:+4858899 | None:intergenic |
| CCCGTCAGTATATTTGAAAG+TGG | 0.536737 | 3.1:-4856847 | MS.gene56261:intron |
| CACACTTCTACATATTGGGA+TGG | 0.538237 | 3.1:-4858173 | MS.gene56261:CDS |
| GATTGCCTGCTATTGAATCT+GGG | 0.542360 | 3.1:-4854253 | MS.gene56261:CDS |
| CCTTGAGCAGTTGGTCAGCA+TGG | 0.542646 | 3.1:+4852942 | None:intergenic |
| CCATGCTGACCAACTGCTCA+AGG | 0.543673 | 3.1:-4852942 | MS.gene56261:intron |
| CAGAACTGCATGTCATAGAT+GGG | 0.551703 | 3.1:-4856267 | MS.gene56261:CDS |
| TACGTCATACCTTGAGCAGT+TGG | 0.553460 | 3.1:+4852933 | None:intergenic |
| ACCACTTTCAAATATACTGA+CGG | 0.555215 | 3.1:+4856846 | None:intergenic |
| ACATCTCCGATCACTACACT+AGG | 0.562845 | 3.1:-4859003 | MS.gene56261:CDS |
| TGGCGCTGTGTATACCAGTA+AGG | 0.573322 | 3.1:+4854770 | None:intergenic |
| TGTGTCATCGGTGTTCAGAA+AGG | 0.576042 | 3.1:-4858878 | MS.gene56261:CDS |
| AAAGCTATTGAAGATTTCAA+CGG | 0.576701 | 3.1:+4858850 | None:intergenic |
| CTTATGATAGACATACCAGA+AGG | 0.579441 | 3.1:-4851697 | MS.gene56261:CDS |
| CACAACAACGCCACCAACAA+CGG | 0.590871 | 3.1:-4858947 | MS.gene56261:CDS |
| CCTCAATAGTATAGCTTGAG+CGG | 0.592064 | 3.1:+4856224 | None:intergenic |
| TTGTTGGTGGCGTTGTTGTG+AGG | 0.596254 | 3.1:+4858950 | None:intergenic |
| TAGTGCAATGAATGATTTGG+TGG | 0.596419 | 3.1:-4851735 | MS.gene56261:CDS |
| CTTAATAGTGAATTCTCACA+AGG | 0.596590 | 3.1:+4854299 | None:intergenic |
| GCTGCTGAACCACCATCAGA+TGG | 0.598136 | 3.1:+4856050 | None:intergenic |
| CATCCCAATATGTAGAAGTG+TGG | 0.603208 | 3.1:+4858174 | None:intergenic |
| CTTAACCCTAGTGTAGTGAT+CGG | 0.604725 | 3.1:+4858997 | None:intergenic |
| CATCTAAAGCCATCTGATGG+TGG | 0.612178 | 3.1:-4856059 | MS.gene56261:CDS |
| GAGATGTTAACGATCACAAG+TGG | 0.615419 | 3.1:+4859019 | None:intergenic |
| AGCAGTTGGTCAGCATGGCA+AGG | 0.615730 | 3.1:+4852947 | None:intergenic |
| TTCAACTGGAAGCGATGCAG+AGG | 0.617753 | 3.1:-4858148 | MS.gene56261:CDS |
| TGCAATCAATCACTCACAGA+AGG | 0.619862 | 3.1:-4856875 | MS.gene56261:CDS |
| TGAAGACTGTTGAAGCAGAA+CGG | 0.622175 | 3.1:-4856103 | MS.gene56261:intron |
| CACTTTCAAATATACTGACG+GGG | 0.636409 | 3.1:+4856848 | None:intergenic |
| GATAGACATACCAGAAGGGG+TGG | 0.636724 | 3.1:-4851692 | MS.gene56261:CDS |
| GCTCATCTAAAGCCATCTGA+TGG | 0.640687 | 3.1:-4856062 | MS.gene56261:CDS |
| TTATGATAGACATACCAGAA+GGG | 0.641065 | 3.1:-4851696 | MS.gene56261:CDS |
| CATCTCCGATCACTACACTA+GGG | 0.644010 | 3.1:-4859002 | MS.gene56261:CDS |
| ATGGCTTCTTCATCGAGCAG+CGG | 0.645057 | 3.1:-4859061 | MS.gene56261:CDS |
| GAGCGGACAAAGATAAGCTG+TGG | 0.650130 | 3.1:+4856241 | None:intergenic |
| TATGATAGACATACCAGAAG+GGG | 0.651893 | 3.1:-4851695 | MS.gene56261:CDS |
| AACAACGCCACCAACAACGG+CGG | 0.667210 | 3.1:-4858944 | MS.gene56261:CDS |
| TGTTAACGATCACAAGTGGG+TGG | 0.675139 | 3.1:+4859023 | None:intergenic |
| AGATGTTAACGATCACAAGT+GGG | 0.688403 | 3.1:+4859020 | None:intergenic |
| ACACAACCAAAGACACGTGG+CGG | 0.700068 | 3.1:+4858896 | None:intergenic |
| ATGACACAACCAAAGACACG+TGG | 0.743645 | 3.1:+4858893 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 4851673 | 4859083 | 4851673 | ID=MS.gene56261 |
| chr3.1 | mRNA | 4851673 | 4859083 | 4851673 | ID=MS.gene56261.t1;Parent=MS.gene56261 |
| chr3.1 | exon | 4858783 | 4859083 | 4858783 | ID=MS.gene56261.t1.exon1;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4858783 | 4859083 | 4858783 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4858122 | 4858210 | 4858122 | ID=MS.gene56261.t1.exon2;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4858122 | 4858210 | 4858122 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4856848 | 4856941 | 4856848 | ID=MS.gene56261.t1.exon3;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4856848 | 4856941 | 4856848 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4856225 | 4856286 | 4856225 | ID=MS.gene56261.t1.exon4;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4856225 | 4856286 | 4856225 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4856042 | 4856120 | 4856042 | ID=MS.gene56261.t1.exon5;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4856042 | 4856120 | 4856042 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4854710 | 4854802 | 4854710 | ID=MS.gene56261.t1.exon6;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4854710 | 4854802 | 4854710 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4854229 | 4854329 | 4854229 | ID=MS.gene56261.t1.exon7;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4854229 | 4854329 | 4854229 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4852943 | 4852998 | 4852943 | ID=MS.gene56261.t1.exon8;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4852943 | 4852998 | 4852943 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| chr3.1 | exon | 4851673 | 4851754 | 4851673 | ID=MS.gene56261.t1.exon9;Parent=MS.gene56261.t1 |
| chr3.1 | CDS | 4851673 | 4851754 | 4851673 | ID=cds.MS.gene56261.t1;Parent=MS.gene56261.t1 |
| Gene Sequence |
| Protein sequence |
