Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63516.t1 | XP_013458821.1 | 99.4 | 318 | 2 | 0 | 1 | 318 | 1 | 318 | 2.60E-152 | 548.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63516.t1 | Q8W206 | 72.8 | 320 | 82 | 2 | 1 | 318 | 1 | 317 | 2.4e-125 | 449.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63516.t1 | I3T146 | 99.4 | 318 | 2 | 0 | 1 | 318 | 1 | 318 | 1.9e-152 | 548.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|
PPI:
| Gene1 | Gene2 | Type |
|---|---|---|
| MS.gene63516 | MS.gene009461 | PPI |
| MS.gene07775 | MS.gene63516 | PPI |
| MS.gene63516 | MS.gene011205 | PPI |
| MS.gene63516 | MS.gene56261 | PPI |
| MS.gene37341 | MS.gene63516 | PPI |
| MS.gene51629 | MS.gene63516 | PPI |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63516.t1 | MTR_3g009270 | 99.371 | 318 | 2 | 0 | 1 | 318 | 1 | 318 | 0.0 | 654 |
| MS.gene63516.t1 | MTR_5g081980 | 26.014 | 296 | 181 | 6 | 13 | 299 | 19 | 285 | 3.87e-23 | 97.4 |
| MS.gene63516.t1 | MTR_3g070180 | 25.574 | 305 | 189 | 6 | 4 | 299 | 10 | 285 | 1.74e-22 | 95.9 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene63516.t1 | AT5G56280 | 80.938 | 320 | 56 | 2 | 1 | 318 | 1 | 317 | 0.0 | 535 |
| MS.gene63516.t1 | AT4G26430 | 77.743 | 319 | 68 | 2 | 1 | 318 | 1 | 317 | 0.0 | 520 |
| MS.gene63516.t1 | AT5G05780 | 24.915 | 293 | 185 | 5 | 13 | 299 | 19 | 282 | 3.68e-23 | 97.8 |
| MS.gene63516.t1 | AT5G05780 | 25.000 | 296 | 184 | 6 | 13 | 299 | 19 | 285 | 8.04e-23 | 96.7 |
| MS.gene63516.t1 | AT3G11270 | 24.915 | 293 | 185 | 5 | 13 | 299 | 19 | 282 | 5.44e-22 | 94.4 |
| MS.gene63516.t1 | AT3G11270 | 25.000 | 296 | 184 | 6 | 13 | 299 | 19 | 285 | 1.25e-21 | 93.6 |
Find 70 sgRNAs with CRISPR-Local
Find 0 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| CTTCCACCACCACGTGTCTT+TGG | 0.252475 | 3.3:-4702686 | MS.gene63516:CDS |
| TATCTTCTTGCTATGGAAAA+AGG | 0.264053 | 3.3:-4698303 | MS.gene63516:intron |
| GCAGTGGCTGCTAATCTTAC+TGG | 0.283173 | 3.3:-4698378 | MS.gene63516:intron |
| TTTCCACACTTCTACATATT+GGG | 0.288492 | 3.3:-4701970 | MS.gene63516:CDS |
| AGCGGTTCGTCCACCCCTTC+TGG | 0.317686 | 3.3:+4695321 | None:intergenic |
| TTCTGTGAGTGATTGATTGC+AGG | 0.321468 | 3.3:+4700433 | None:intergenic |
| TCAGAACTGCATGTCATAGA+TGG | 0.338928 | 3.3:-4699825 | MS.gene63516:intron |
| AGATTGCCTGCTATTGAATC+TGG | 0.341800 | 3.3:-4697292 | MS.gene63516:CDS |
| CTTGTGAGAATTCACTATTA+AGG | 0.390102 | 3.3:-4697336 | MS.gene63516:CDS |
| AATGCTTCATAGCAGAATAA+AGG | 0.397301 | 3.3:-4698337 | MS.gene63516:CDS |
| CAATAGCAGGCAATCTTCTC+AGG | 0.404144 | 3.3:+4697299 | None:intergenic |
| ATTTCAAGATGATTTCTTGA+TGG | 0.415768 | 3.3:-4697266 | MS.gene63516:intron |
| CGATCAAGCGAATGAGTAGA+AGG | 0.429828 | 3.3:+4702596 | None:intergenic |
| CTGTAGTGCAATGAATGATT+TGG | 0.433232 | 3.3:-4695377 | MS.gene63516:intron |
| AGCTTTGTGAATATGCATAT+CGG | 0.440439 | 3.3:+4701915 | None:intergenic |
| CGTGTCTTTGGTTGTGTCAT+CGG | 0.442356 | 3.3:-4702674 | MS.gene63516:CDS |
| AGAGTTCTTGCTTCTTCTCA+AGG | 0.446432 | 3.3:+4702567 | None:intergenic |
| ATATTGGGATGGTATTCAAC+TGG | 0.453349 | 3.3:-4701955 | MS.gene63516:CDS |
| GGCAAGGTAACTGATCAATA+AGG | 0.459470 | 3.3:+4696579 | None:intergenic |
| GACACCGTCACCGCCGTTGT+TGG | 0.467752 | 3.3:+4702718 | None:intergenic |
| CCGCTCAAGCTATACTATTG+AGG | 0.482131 | 3.3:-4699781 | MS.gene63516:intron |
| AATTTCCCAGATTCAATAGC+AGG | 0.482170 | 3.3:+4697286 | None:intergenic |
| GATGGATATCAATGAAAGTC+CGG | 0.506227 | 3.3:-4700473 | MS.gene63516:CDS |
| GGGTTCAGAAGAACATAAAC+CGG | 0.509577 | 3.3:+4700454 | None:intergenic |
| TCTTGCTTCTTCTCAAGGAA+AGG | 0.513508 | 3.3:+4702572 | None:intergenic |
| TCTGTGAGTGATTGATTGCA+GGG | 0.515077 | 3.3:+4700434 | None:intergenic |
| TTGTTGTGAGGAGCGTGTGT+AGG | 0.523571 | 3.3:+4702746 | None:intergenic |
| ACATCACTATCTTCTTGCTA+TGG | 0.532058 | 3.3:-4698310 | MS.gene63516:CDS |
| GCAACCAACAACGGCGGTGA+CGG | 0.532811 | 3.3:-4702722 | MS.gene63516:CDS |
| CACACTTCTACATATTGGGA+TGG | 0.538237 | 3.3:-4701966 | MS.gene63516:CDS |
| CCTCTCAGTATATTTGAAAG+TGG | 0.540111 | 3.3:-4700403 | MS.gene63516:intron |
| GATTGCCTGCTATTGAATCT+GGG | 0.542360 | 3.3:-4697291 | MS.gene63516:CDS |
| CCTTGAGCAGTTGGTCAGCA+TGG | 0.542646 | 3.3:+4696558 | None:intergenic |
| CAACCAAAGACACGTGGTGG+TGG | 0.543176 | 3.3:+4702683 | None:intergenic |
| CCATGCTGACCAACTGCTCA+AGG | 0.543673 | 3.3:-4696558 | MS.gene63516:intron |
| TGTGTCATACCTTGAGCAGT+TGG | 0.548039 | 3.3:+4696549 | None:intergenic |
| CAGAACTGCATGTCATAGAT+GGG | 0.551703 | 3.3:-4699824 | MS.gene63516:CDS |
| TGTTGTGAGGAGCGTGTGTA+GGG | 0.553440 | 3.3:+4702747 | None:intergenic |
| ACATCTCCGATCACTACACT+AGG | 0.562845 | 3.3:-4702787 | MS.gene63516:CDS |
| TGTGTCATCGGTGTTCAGAA+AGG | 0.576042 | 3.3:-4702662 | MS.gene63516:CDS |
| CTTATGATAGACATACCAGA+AGG | 0.579441 | 3.3:-4695336 | MS.gene63516:CDS |
| CCTCAATAGTATAGCTTGAG+CGG | 0.592064 | 3.3:+4699781 | None:intergenic |
| TAGTGCAATGAATGATTTGG+TGG | 0.596419 | 3.3:-4695374 | MS.gene63516:CDS |
| CTTAATAGTGAATTCTCACA+AGG | 0.596590 | 3.3:+4697337 | None:intergenic |
| GCTGCTGAACCACCATCAGA+TGG | 0.598136 | 3.3:+4699607 | None:intergenic |
| GAGATGTTAACGATCACTAG+TGG | 0.599766 | 3.3:+4702803 | None:intergenic |
| CATCCCAATATGTAGAAGTG+TGG | 0.603208 | 3.3:+4701967 | None:intergenic |
| CTTAACCCTAGTGTAGTGAT+CGG | 0.604725 | 3.3:+4702781 | None:intergenic |
| CATCTAAAGCCATCTGATGG+TGG | 0.612178 | 3.3:-4699616 | MS.gene63516:CDS |
| CACTTTCAAATATACTGAGA+GGG | 0.615438 | 3.3:+4700404 | None:intergenic |
| AGCAGTTGGTCAGCATGGCA+AGG | 0.615730 | 3.3:+4696563 | None:intergenic |
| TTGTTGGTTGCGTTGTTGTG+AGG | 0.616679 | 3.3:+4702734 | None:intergenic |
| TTCAACTGGAAGCGATGCAG+AGG | 0.617753 | 3.3:-4701941 | MS.gene63516:CDS |
| CACAACAACGCAACCAACAA+CGG | 0.618659 | 3.3:-4702731 | MS.gene63516:CDS |
| TGCAATCAATCACTCACAGA+AGG | 0.619862 | 3.3:-4700431 | MS.gene63516:CDS |
| TGAAGACTGTTGAAGCAGAA+CGG | 0.622175 | 3.3:-4699660 | MS.gene63516:intron |
| GATAGACATACCAGAAGGGG+TGG | 0.636724 | 3.3:-4695331 | MS.gene63516:CDS |
| GCTCATCTAAAGCCATCTGA+TGG | 0.640687 | 3.3:-4699619 | MS.gene63516:CDS |
| TTATGATAGACATACCAGAA+GGG | 0.641065 | 3.3:-4695335 | MS.gene63516:CDS |
| CATCTCCGATCACTACACTA+GGG | 0.644010 | 3.3:-4702786 | MS.gene63516:CDS |
| ATGGCTTCTTCATCGAGCAG+CGG | 0.649868 | 3.3:-4702845 | MS.gene63516:CDS |
| GAGCGGACAAAGATAAGCTG+TGG | 0.650130 | 3.3:+4699798 | None:intergenic |
| AGATGTTAACGATCACTAGT+GGG | 0.650152 | 3.3:+4702804 | None:intergenic |
| TATGATAGACATACCAGAAG+GGG | 0.651893 | 3.3:-4695334 | MS.gene63516:CDS |
| CCACTTTCAAATATACTGAG+AGG | 0.672734 | 3.3:+4700403 | None:intergenic |
| TGTTAACGATCACTAGTGGG+TGG | 0.675139 | 3.3:+4702807 | None:intergenic |
| AGGCGACATTCACATGAAAG+CGG | 0.689954 | 3.3:+4695303 | None:intergenic |
| ACACAACCAAAGACACGTGG+TGG | 0.692490 | 3.3:+4702680 | None:intergenic |
| AACAACGCAACCAACAACGG+CGG | 0.724981 | 3.3:-4702728 | MS.gene63516:CDS |
| ATGACACAACCAAAGACACG+TGG | 0.747317 | 3.3:+4702677 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.3 | gene | 4695312 | 4702867 | 4695312 | ID=MS.gene63516 |
| chr3.3 | mRNA | 4695312 | 4702867 | 4695312 | ID=MS.gene63516.t1;Parent=MS.gene63516 |
| chr3.3 | exon | 4702567 | 4702867 | 4702567 | ID=MS.gene63516.t1.exon1;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4702567 | 4702867 | 4702567 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4701915 | 4702003 | 4701915 | ID=MS.gene63516.t1.exon2;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4701915 | 4702003 | 4701915 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4700404 | 4700497 | 4700404 | ID=MS.gene63516.t1.exon3;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4700404 | 4700497 | 4700404 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4699782 | 4699843 | 4699782 | ID=MS.gene63516.t1.exon4;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4699782 | 4699843 | 4699782 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4699599 | 4699677 | 4699599 | ID=MS.gene63516.t1.exon5;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4699599 | 4699677 | 4699599 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4698304 | 4698396 | 4698304 | ID=MS.gene63516.t1.exon6;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4698304 | 4698396 | 4698304 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4697267 | 4697367 | 4697267 | ID=MS.gene63516.t1.exon7;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4697267 | 4697367 | 4697267 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4696559 | 4696614 | 4696559 | ID=MS.gene63516.t1.exon8;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4696559 | 4696614 | 4696559 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| chr3.3 | exon | 4695312 | 4695393 | 4695312 | ID=MS.gene63516.t1.exon9;Parent=MS.gene63516.t1 |
| chr3.3 | CDS | 4695312 | 4695393 | 4695312 | ID=cds.MS.gene63516.t1;Parent=MS.gene63516.t1 |
| Gene Sequence |
| Protein sequence |
