AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene56269

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56269.t1 MTR_3g009530 98.419 759 10 1 1 759 1 757 0.0 1523
MS.gene56269.t1 MTR_3g009530 99.147 586 5 0 174 759 1 586 0.0 1188
MS.gene56269.t1 MTR_5g099290 38.283 734 380 15 35 732 2 698 1.58e-120 378
MS.gene56269.t1 MTR_5g099290 38.283 734 380 15 35 732 2 698 4.45e-120 377
MS.gene56269.t1 MTR_5g099290 39.280 667 344 12 35 666 2 642 2.34e-115 363
MS.gene56269.t1 MTR_5g099290 36.799 731 367 15 35 732 2 670 1.62e-109 348
MS.gene56269.t1 MTR_5g099290 37.651 664 331 12 35 666 2 614 2.27e-104 333
MS.gene56269.t1 MTR_5g099290 39.149 470 251 7 35 488 2 452 6.29e-73 245
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene56269.t1 AT1G55620 69.920 748 188 9 39 759 44 781 0.0 996
MS.gene56269.t1 AT1G55620 73.019 593 142 4 177 759 1 585 0.0 840
MS.gene56269.t1 AT4G35440 40.895 626 329 12 103 709 68 671 8.09e-134 413
MS.gene56269.t1 AT4G35440 40.895 626 329 12 103 709 68 671 3.63e-133 412

Find 205 sgRNAs with CRISPR-Local

Find 0 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GTGCTGTTGGGCTGGCCATT+TGG 0.154869 3.1:-4929599 MS.gene56269:CDS
CTTCATTCATTTGATTCTTC+TGG 0.175525 3.1:-4935128 MS.gene56269:CDS
ATAATATTCCAGGATATTTA+AGG 0.176331 3.1:+4930523 None:intergenic
TGCTGTTGGGCTGGCCATTT+GGG 0.181190 3.1:-4929598 MS.gene56269:CDS
AATCGATGAAGAACTATTTC+TGG 0.200927 3.1:-4929427 MS.gene56269:CDS
GCCATGGCCAAATTAGTATT+TGG 0.216857 3.1:+4928474 None:intergenic
ACAGCTCTTTGCAAGGGCTC+TGG 0.217875 3.1:-4930396 MS.gene56269:CDS
CTGGCTTCGTTTGCAGAGAT+TGG 0.220386 3.1:-4933381 MS.gene56269:CDS
TTGTTATCCAAATACTAATT+TGG 0.228515 3.1:-4928481 MS.gene56269:CDS
GACTATATCCAGGAGAAATT+TGG 0.229814 3.1:-4930600 MS.gene56269:CDS
AACTGTATCAAATGTCTTAC+AGG 0.230240 3.1:-4931543 MS.gene56269:CDS
CTACCTTTATACCTGATATT+AGG 0.275635 3.1:-4930687 MS.gene56269:CDS
CTTAGGTACTGGCTGTTCAT+TGG 0.283175 3.1:-4933177 MS.gene56269:CDS
TACAACTGCCATGATTATAT+TGG 0.284492 3.1:-4931582 MS.gene56269:CDS
AATATCCTGGAATATTATAC+TGG 0.291535 3.1:-4930518 MS.gene56269:CDS
CTTGTTGCTGCTTTCAATAA+AGG 0.292178 3.1:-4935026 MS.gene56269:CDS
GGCGGTGGTGGTGGCGGTAG+TGG 0.299309 3.1:-4935302 MS.gene56269:CDS
TGCAAGGGCTCTGGGCTTGT+AGG 0.300501 3.1:-4930387 MS.gene56269:CDS
TTGCTCCTTGGTTGTCTCCT+TGG 0.310407 3.1:-4935065 MS.gene56269:CDS
AACGATCAACTCCGTCTTCT+AGG 0.310860 3.1:-4935389 MS.gene56269:CDS
ACCATGACTCCTCCGGTTAC+AGG 0.314186 3.1:+4933329 None:intergenic
GAACACATCGCCGGAGTTAA+TGG 0.314664 3.1:-4935323 MS.gene56269:CDS
CAGCTCTTTGCAAGGGCTCT+GGG 0.316199 3.1:-4930395 MS.gene56269:CDS
GGCTTCAATGCAGCAGTTGC+AGG 0.317536 3.1:-4931665 MS.gene56269:CDS
GTGCTTCTGCTCCTGGAATA+TGG 0.331356 3.1:-4930455 MS.gene56269:CDS
ACTATTTCTGGACAAACTTA+AGG 0.331504 3.1:-4929415 MS.gene56269:intron
CAATGACTCGTTTGGTTGCT+TGG 0.331580 3.1:-4930638 MS.gene56269:CDS
ATAATCATGGCAGTTGTAAA+TGG 0.332811 3.1:+4931587 None:intergenic
TTGTTGCTGCTTTCAATAAA+GGG 0.333792 3.1:-4935025 MS.gene56269:CDS
TCCACCTGGTAAAAGAAATT+TGG 0.333930 3.1:-4929541 MS.gene56269:CDS
GTGTGGTTTACTAGAAATAT+TGG 0.355175 3.1:-4933297 MS.gene56269:CDS
GCTGCAGTTGGTGCTGTGTT+TGG 0.356598 3.1:-4930333 MS.gene56269:CDS
TTCCTCTTGTCAGGTTTCTC+AGG 0.357791 3.1:-4929040 MS.gene56269:intron
TTAGGTACTGGCTGTTCATT+GGG 0.359687 3.1:-4933176 MS.gene56269:CDS
GCAGCAGCTGGAATTTCTTC+AGG 0.362055 3.1:-4933050 MS.gene56269:intron
GGCGGTAGTGGGAGTGGTAA+AGG 0.362636 3.1:-4935290 MS.gene56269:CDS
AAAATTTCTCAGGGTGCTGT+TGG 0.367341 3.1:-4929612 MS.gene56269:intron
TGCCCTGCCATAGGTGGTTT+AGG 0.367716 3.1:-4930561 MS.gene56269:CDS
TTCTCAGGGTGCTGTTGGGC+TGG 0.372502 3.1:-4929607 MS.gene56269:intron
GGTTTAGAACTCTCCATTGT+TGG 0.374374 3.1:-4929471 MS.gene56269:CDS
ATTCCTAATATCAGGTATAA+AGG 0.376141 3.1:+4930684 None:intergenic
GGCCATGGCCAGAGAGTTGA+TGG 0.377024 3.1:-4928460 MS.gene56269:CDS
GCCATAGGTGGTTTAGGAGC+TGG 0.378901 3.1:-4930555 MS.gene56269:CDS
AGAGATATGTTGAAATTATC+AGG 0.385849 3.1:-4935263 MS.gene56269:CDS
AGGAGCAACAAAGCCCATTC+AGG 0.387645 3.1:+4935080 None:intergenic
GGTTCCCTCAGTGACAAATC+AGG 0.389342 3.1:-4929577 MS.gene56269:CDS
ACAGAAAACAATTTGACAAA+TGG 0.391756 3.1:-4927822 MS.gene56269:CDS
TAAGAGCGACTCAGATCCTT+TGG 0.394845 3.1:-4928839 MS.gene56269:CDS
GAATGGGCTTTGTTGCTCCT+TGG 0.397932 3.1:-4935077 MS.gene56269:CDS
TGACATATGGTGACATTAGA+AGG 0.401530 3.1:-4928891 MS.gene56269:CDS
GGCTTACCAGAGCATAAGCC+TGG 0.405869 3.1:+4930250 None:intergenic
GGAAAGAGTGCTTCTGCTCC+TGG 0.406413 3.1:-4930462 MS.gene56269:CDS
CGGGCACTGTAAAAGCTGAT+TGG 0.411686 3.1:+4931516 None:intergenic
TGTCTCCTTGGTCTTGCCAC+TGG 0.413211 3.1:-4935053 MS.gene56269:CDS
ATATCAACACTAGGACCTTC+AGG 0.417374 3.1:+4933152 None:intergenic
AAGTCAAAACCTTGTCTTTG+AGG 0.418710 3.1:+4933248 None:intergenic
CTTGAAGGGATATTGACATA+TGG 0.419647 3.1:-4928904 MS.gene56269:CDS
AGAAGTCTCATTAGACTTCT+CGG 0.421457 3.1:+4928861 None:intergenic
TGTGCAACAGCAGCATTTCC+AGG 0.423733 3.1:+4930276 None:intergenic
GTTGCAGCTGGTGCAGCAGC+TGG 0.424808 3.1:-4933062 MS.gene56269:CDS
GCGGTGGTGGTGGCGGTAGT+GGG 0.426567 3.1:-4935301 MS.gene56269:CDS
TCCTTCATTATGACGAACTA+TGG 0.426948 3.1:-4928356 MS.gene56269:CDS
ATCATTGCCCTTAAATATCC+TGG 0.429383 3.1:-4930531 MS.gene56269:CDS
ACGAACTATGGCACTGTCTC+AGG 0.429918 3.1:-4928344 MS.gene56269:intron
CACACAGCATTCCTAATATC+AGG 0.431743 3.1:+4930676 None:intergenic
AAATTTCTCAGGGTGCTGTT+GGG 0.433280 3.1:-4929611 MS.gene56269:intron
CTGATAGTGCACCTCCTGAA+TGG 0.436429 3.1:-4935094 MS.gene56269:CDS
TCAAATAATGAAGATGATGA+TGG 0.439967 3.1:-4929498 MS.gene56269:CDS
CTCCATCAACTCTCTGGCCA+TGG 0.440124 3.1:+4928458 None:intergenic
GGAGTTAATGGCGGTGGTGG+TGG 0.440137 3.1:-4935311 MS.gene56269:CDS
TGAGTTAGAAGCCATATTCC+AGG 0.441628 3.1:+4930444 None:intergenic
AGCCAGGCAGCACCCTCATT+CGG 0.445698 3.1:+4933398 None:intergenic
GCAGTTGGTGCTGTGTTTGG+AGG 0.445882 3.1:-4930330 MS.gene56269:CDS
GTTATCAATTCAGCAATTCC+TGG 0.446728 3.1:-4930294 MS.gene56269:CDS
AAACCCCAGTATAATATTCC+AGG 0.447643 3.1:+4930513 None:intergenic
GGTGGTGGCGGTAGTGGGAG+TGG 0.449866 3.1:-4935296 MS.gene56269:CDS
CCATAGGTGGTTTAGGAGCT+GGG 0.452875 3.1:-4930554 MS.gene56269:CDS
ATCGGAAAGTCGTGCGCCAA+TGG 0.454778 3.1:-4933131 MS.gene56269:CDS
TAATATTCCAGGATATTTAA+GGG 0.456614 3.1:+4930524 None:intergenic
CTTGGCCTCCATCAACTCTC+TGG 0.457217 3.1:+4928452 None:intergenic
GAACTCTCCATTGTTGGAGA+TGG 0.461096 3.1:-4929465 MS.gene56269:CDS
AGTCAAAACCTTGTCTTTGA+GGG 0.462702 3.1:+4933249 None:intergenic
GTTAATGGCGGTGGTGGTGG+CGG 0.462911 3.1:-4935308 MS.gene56269:CDS
ATTCAAGCTGCAGTTACCTT+AGG 0.464362 3.1:-4933194 MS.gene56269:CDS
GTGGAAGAAATTCTACGTAC+TGG 0.465680 3.1:-4930483 MS.gene56269:CDS
CAAGGGTGTTAAGCAATTAC+CGG 0.469037 3.1:-4928433 MS.gene56269:CDS
TGCTGCTGTTGCACAACCCC+AGG 0.472828 3.1:-4930268 MS.gene56269:CDS
AGGTGCATGTAATACATGAA+TGG 0.473264 3.1:-4933436 MS.gene56269:CDS
CCCAGCTCCTAAACCACCTA+TGG 0.473469 3.1:+4930554 None:intergenic
CTCCGAATGAGGGTGCTGCC+TGG 0.475061 3.1:-4933400 MS.gene56269:CDS
GCGGTAGTGGGAGTGGTAAA+GGG 0.475130 3.1:-4935289 MS.gene56269:CDS
TTCATCGATTTCTTCAAGCT+CGG 0.478662 3.1:+4929440 None:intergenic
CAGAAAACAATTTGACAAAT+GGG 0.478761 3.1:-4927821 MS.gene56269:CDS
ATGTAATACATGAATGGGTA+TGG 0.481494 3.1:-4933430 MS.gene56269:CDS
TGGCTTCGTTTGCAGAGATT+GGG 0.483027 3.1:-4933380 MS.gene56269:CDS
TGATAGTGCACCTCCTGAAT+GGG 0.483817 3.1:-4935093 MS.gene56269:CDS
GGCTGTTCATTGGGTCCTGA+AGG 0.484109 3.1:-4933167 MS.gene56269:CDS
TGCAGAGATTGGGTGATACA+TGG 0.484884 3.1:-4933370 MS.gene56269:CDS
AGCAGATTTCAACTCGTACT+CGG 0.486135 3.1:+4931496 None:intergenic
GAGAGGAAGCGAAGAATTGT+CGG 0.486398 3.1:-4928381 MS.gene56269:CDS
GGTGCATGTAATACATGAAT+GGG 0.486735 3.1:-4933435 MS.gene56269:CDS
TACGCACCGAGTTTAATGAT+TGG 0.489864 3.1:-4930357 MS.gene56269:CDS
TAGAATACTGCTTCCTCTCA+TGG 0.492339 3.1:-4929837 MS.gene56269:intron
AAGCGTGGCAATGACTCGTT+TGG 0.492664 3.1:-4930646 MS.gene56269:CDS
CATCATTAATGAGAATCCAT+TGG 0.492781 3.1:+4933115 None:intergenic
GAAGGTCCTAGTGTTGATAT+CGG 0.495307 3.1:-4933149 MS.gene56269:CDS
TTCCCCTGATTTGTCACTGA+GGG 0.495318 3.1:+4929573 None:intergenic
GCCGGAGTTAATGGCGGTGG+TGG 0.496160 3.1:-4935314 MS.gene56269:CDS
GGGCACTGTAAAAGCTGATT+GGG 0.497926 3.1:+4931517 None:intergenic
ACTGTAGTCTGCCCTGCCAT+AGG 0.499166 3.1:-4930570 MS.gene56269:CDS
ATGGGCTGGTACTCCGAATG+AGG 0.500266 3.1:-4933411 MS.gene56269:CDS
GCTTGAATAGCCTTTACCGT+TGG 0.500425 3.1:+4933209 None:intergenic
CAATCTCTGCAAACGAAGCC+AGG 0.502178 3.1:+4933382 None:intergenic
CGGTAATTGCTTAACACCCT+TGG 0.502192 3.1:+4928434 None:intergenic
AACAGAAGCCAATATAATCA+TGG 0.502461 3.1:+4931574 None:intergenic
TCCAAATACTAATTTGGCCA+TGG 0.508333 3.1:-4928475 MS.gene56269:CDS
TTTCCACCGCGTTTAACTAC+CGG 0.509290 3.1:+4928414 None:intergenic
GTTTCCTCTGTTTGTACTCG+AGG 0.510022 3.1:-4928540 MS.gene56269:CDS
CAATGGATTCTCATTAATGA+TGG 0.511491 3.1:-4933114 MS.gene56269:CDS
GCTCCTAAACCACCTATGGC+AGG 0.513781 3.1:+4930558 None:intergenic
CTCCGGTTACAGGTATCAAA+AGG 0.516383 3.1:+4933339 None:intergenic
AATATTGGATCAAATAAAGC+AGG 0.518607 3.1:-4933282 MS.gene56269:CDS
TATTGCCACAGCTCTTTGCA+AGG 0.519958 3.1:-4930403 MS.gene56269:CDS
AAAATAGCACTTGTTGCAGC+TGG 0.520530 3.1:-4933074 MS.gene56269:CDS
ACCTGTAACCGGAGGAGTCA+TGG 0.521632 3.1:-4933330 MS.gene56269:CDS
ACCACCACCGCCATTAACTC+CGG 0.524738 3.1:+4935313 None:intergenic
ATATCCTGGAATATTATACT+GGG 0.526329 3.1:-4930517 MS.gene56269:CDS
TGGCTTCTAACTCAATTAGC+AGG 0.529356 3.1:-4930435 MS.gene56269:CDS
AACTCAATTAGCAGGTGCTA+AGG 0.529397 3.1:-4930427 MS.gene56269:CDS
CTCGGAACCATCTCCAACAA+TGG 0.532176 3.1:+4929458 None:intergenic
GCAGATTTCAACTCGTACTC+GGG 0.532249 3.1:+4931497 None:intergenic
CAATGAACAGCCAGTACCTA+AGG 0.533126 3.1:+4933178 None:intergenic
CAGAGAGTTGATGGAGGCCA+AGG 0.536310 3.1:-4928451 MS.gene56269:CDS
TTTGATAATCTAACTTTCTG+TGG 0.537945 3.1:+4927850 None:intergenic
GCTGCAGTTACCTTAGGTAC+TGG 0.538441 3.1:-4933188 MS.gene56269:CDS
TAGGAAGGCCAAATTTCTCC+TGG 0.539754 3.1:+4930592 None:intergenic
AGGAATCTATCCAACGGTAA+AGG 0.540393 3.1:-4933219 MS.gene56269:CDS
GCCATAGTTCGTCATAATGA+AGG 0.541299 3.1:+4928355 None:intergenic
TTAACTTGTGCAGGTTGGAA+TGG 0.542954 3.1:-4929933 MS.gene56269:intron
TGTTGCTGCTTTCAATAAAG+GGG 0.543037 3.1:-4935024 MS.gene56269:intron
TGTCACTGAGGGAACCCAAA+TGG 0.544172 3.1:+4929584 None:intergenic
CACGGATCGGAACACATCGC+CGG 0.548171 3.1:-4935332 MS.gene56269:CDS
TTAATGATTGGTGCTGCAGT+TGG 0.548394 3.1:-4930345 MS.gene56269:CDS
CAAATAATGAAGATGATGAT+GGG 0.550654 3.1:-4929497 MS.gene56269:CDS
ATTGCCACAGCTCTTTGCAA+GGG 0.555909 3.1:-4930402 MS.gene56269:CDS
AAGGGCTCTGGGCTTGTAGG+TGG 0.557216 3.1:-4930384 MS.gene56269:CDS
ACAACCCCAGGCTTATGCTC+TGG 0.559697 3.1:-4930256 MS.gene56269:intron
ATCGCCGGAGTTAATGGCGG+TGG 0.561251 3.1:-4935317 MS.gene56269:CDS
CATGCCAACCATGACTCCTC+CGG 0.562534 3.1:+4933322 None:intergenic
GTAGTCTGCCCTGCCATAGG+TGG 0.563738 3.1:-4930567 MS.gene56269:CDS
TACGAGTTGAAATCTGCTGC+TGG 0.570488 3.1:-4931491 MS.gene56269:intron
GTTCCCTCAGTGACAAATCA+GGG 0.580531 3.1:-4929576 MS.gene56269:CDS
CTCAGTGACAAATCAGGGGA+AGG 0.581546 3.1:-4929571 MS.gene56269:CDS
GCTTACCAGAGCATAAGCCT+GGG 0.582753 3.1:+4930251 None:intergenic
GTCATGGTTGGCATGATGTG+TGG 0.594764 3.1:-4933314 MS.gene56269:CDS
AGAGCCCTTGCAAAGAGCTG+TGG 0.596197 3.1:+4930398 None:intergenic
GTCAAAACCTTGTCTTTGAG+GGG 0.596299 3.1:+4933250 None:intergenic
TCTTTGAGGGTTACAGATGA+CGG 0.598520 3.1:+4928991 None:intergenic
AGTTATTCCCCTCAAAGACA+AGG 0.598527 3.1:-4933257 MS.gene56269:CDS
AGCTATCGTGGACGAGAACG+TGG 0.600799 3.1:-4928513 MS.gene56269:CDS
ATACTGGGGTTTCACAAATG+TGG 0.606217 3.1:-4930502 MS.gene56269:CDS
TGGGCTGGTACTCCGAATGA+GGG 0.609332 3.1:-4933410 MS.gene56269:CDS
GGAGAATTCTCTGCACGAAG+AGG 0.611903 3.1:+4931611 None:intergenic
GGAGACCAGTGGCAAGACCA+AGG 0.612140 3.1:+4935048 None:intergenic
CTCCTAAACCACCTATGGCA+GGG 0.612594 3.1:+4930559 None:intergenic
AGAAGTAGAACTGAAGTCAA+AGG 0.614115 3.1:+4929881 None:intergenic
GAACGAGACATTGAAGCTCA+CGG 0.614125 3.1:-4935350 MS.gene56269:CDS
TCGCTATTGAAACTGTGCTG+AGG 0.616198 3.1:-4931634 MS.gene56269:CDS
CTTTATTGAAAGCAGCAACA+AGG 0.617678 3.1:+4935027 None:intergenic
AATGAAGATGATGATGGGAA+TGG 0.618213 3.1:-4929492 MS.gene56269:CDS
ATGGCAGGGCAGACTACAGT+AGG 0.622344 3.1:+4930573 None:intergenic
CATGTCGAAATACTATCTGA+AGG 0.623321 3.1:-4929016 MS.gene56269:CDS
CAGCCAGCAAAACTGTGTGC+TGG 0.629332 3.1:-4928947 MS.gene56269:CDS
AGACATTGAAGCTCACGGAT+CGG 0.631337 3.1:-4935345 MS.gene56269:CDS
GGTAAAAGAAATTTGGCGAG+AGG 0.632782 3.1:-4929534 MS.gene56269:CDS
ATCATGGCAGTTGTAAATGG+AGG 0.633666 3.1:+4931590 None:intergenic
GCTGTGTGGCGTTGTAAGCG+TGG 0.636334 3.1:-4930661 MS.gene56269:CDS
AGAGAGTTGATGGAGGCCAA+GGG 0.636629 3.1:-4928450 MS.gene56269:CDS
ACTCGAGGAATGAGCTATCG+TGG 0.638496 3.1:-4928525 MS.gene56269:CDS
AGCAACAAAGCCCATTCAGG+AGG 0.640278 3.1:+4935083 None:intergenic
TGTAATACATGAATGGGTAT+GGG 0.640519 3.1:-4933429 MS.gene56269:CDS
CAGGGCAGACTACAGTAGGA+AGG 0.640537 3.1:+4930577 None:intergenic
CTTCAGATAGTATTTCGACA+TGG 0.649097 3.1:+4929017 None:intergenic
CTTCCCCTGATTTGTCACTG+AGG 0.650403 3.1:+4929572 None:intergenic
ATACATGAATGGGTATGGGC+TGG 0.650440 3.1:-4933425 MS.gene56269:CDS
ACTGTATCAAATGTCTTACA+GGG 0.656078 3.1:-4931542 MS.gene56269:CDS
GTAACCGGAGGAGTCATGGT+TGG 0.664027 3.1:-4933326 MS.gene56269:CDS
GTGGAAATCACAATAGAGAG+AGG 0.664584 3.1:-4928398 MS.gene56269:CDS
CTTCTGATGAACCTAGAAGA+CGG 0.665644 3.1:+4935378 None:intergenic
TCTCATTAGACTTCTCGGAT+CGG 0.666988 3.1:+4928866 None:intergenic
TTCCCTCAGTGACAAATCAG+GGG 0.672221 3.1:-4929575 MS.gene56269:CDS
GCAGCACCAATCATTAAACT+CGG 0.674844 3.1:+4930351 None:intergenic
CTTACCAGAGCATAAGCCTG+GGG 0.679204 3.1:+4930252 None:intergenic
GACTTTCCGATATCAACACT+AGG 0.682433 3.1:+4933143 None:intergenic
AGCAGCAACAAGGAGACCAG+TGG 0.683119 3.1:+4935037 None:intergenic
CAAGACCAAGGAGACAACCA+AGG 0.688884 3.1:+4935060 None:intergenic
GGGAAGGAGAGTGATCCACC+TGG 0.688942 3.1:-4929555 MS.gene56269:CDS
CATGGCCAGAGAGTTGATGG+AGG 0.691504 3.1:-4928457 MS.gene56269:CDS
GCTTGCAGGAATCTATCCAA+CGG 0.696236 3.1:-4933225 MS.gene56269:CDS
TTACCGGTAGTTAAACGCGG+TGG 0.700865 3.1:-4928417 MS.gene56269:CDS
TATCCTGGAATATTATACTG+GGG 0.700995 3.1:-4930516 MS.gene56269:CDS
CACATCGCCGGAGTTAATGG+CGG 0.705098 3.1:-4935320 MS.gene56269:CDS
CAATTACCGGTAGTTAAACG+CGG 0.707073 3.1:-4928420 MS.gene56269:CDS
CATTCCTCGAGTACAAACAG+AGG 0.725332 3.1:+4928536 None:intergenic
CTGATATTAGGAATGCTGTG+TGG 0.784835 3.1:-4930675 MS.gene56269:CDS

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content


Chromosome Type Strat End Strand Name
chr3.1 gene 4927815 4935420 4927815 ID=MS.gene56269
chr3.1 mRNA 4927815 4935420 4927815 ID=MS.gene56269.t1;Parent=MS.gene56269
chr3.1 exon 4935025 4935420 4935025 ID=MS.gene56269.t1.exon1;Parent=MS.gene56269.t1
chr3.1 CDS 4935025 4935420 4935025 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4933051 4933456 4933051 ID=MS.gene56269.t1.exon2;Parent=MS.gene56269.t1
chr3.1 CDS 4933051 4933456 4933051 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4931492 4931686 4931492 ID=MS.gene56269.t1.exon3;Parent=MS.gene56269.t1
chr3.1 CDS 4931492 4931686 4931492 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4930257 4930711 4930257 ID=MS.gene56269.t1.exon4;Parent=MS.gene56269.t1
chr3.1 CDS 4930257 4930711 4930257 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4929838 4929942 4929838 ID=MS.gene56269.t1.exon5;Parent=MS.gene56269.t1
chr3.1 CDS 4929838 4929942 4929838 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4929416 4929622 4929416 ID=MS.gene56269.t1.exon6;Parent=MS.gene56269.t1
chr3.1 CDS 4929416 4929622 4929416 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4928837 4929049 4928837 ID=MS.gene56269.t1.exon7;Parent=MS.gene56269.t1
chr3.1 CDS 4928837 4929049 4928837 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4928345 4928577 4928345 ID=MS.gene56269.t1.exon8;Parent=MS.gene56269.t1
chr3.1 CDS 4928345 4928577 4928345 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
chr3.1 exon 4927815 4927884 4927815 ID=MS.gene56269.t1.exon9;Parent=MS.gene56269.t1
chr3.1 CDS 4927815 4927884 4927815 ID=cds.MS.gene56269.t1;Parent=MS.gene56269.t1
Gene Sequence

>MS.gene56269

ATGCCGGAAAACGATCAACTCCGTCTTCTAGGTTCATCAGAAGATGCAGAACGAGACATTGAAGCTCACGGATCGGAACACATCGCCGGAGTTAATGGCGGTGGTGGTGGCGGTAGTGGGAGTGGTAAAGGGTTGAGAGATATGTTGAAATTATCAGGTCATCATCGTCATAGTTTTAAGCGTTTAGAGAGAGAAAGAGAAAGAGAAAGAGATCGTGATGTTGATAATAATAATAATAATGTGCGTGATTCTTCTCACTCTGAGTTTGATCTTCATTCATTTGATTCTTCTGGTGATGTTCTTGCTGATAGTGCACCTCCTGAATGGGCTTTGTTGCTCCTTGGTTGTCTCCTTGGTCTTGCCACTGGTCTCCTTGTTGCTGCTTTCAATAAAGGGGTTTGTCTATTTTCAATCTTTGAGAATGTTTTGTTTTTTTTGGTTTTTGACATGTTTATGTGATTTATGTGTAGAACATGTTTAGTTTTGTTTGTTGTTGTTGTGTTGAAAATTAATGTTTGAATGATGATCTTAATGGATAGGAGGTGTTAGGAGAAACTGTTATTAGTTAGTTACACTCAATGATCTGGATCTATGAAGCTTCAAGCATAGGCACAGATACCGGATATGACACTAACACATAGACATCGGTAGTAATTTGAAAATTAGCGTGTCTGAATGTGATCTCAATGGAAATGAGCAAAACAAACTACGATATGGTGATAGGAGAAACTGTTACTAGTTAGTTATACTTTCAATGATCTAGATCTACGAAGCACAAAAACTGACACTTAACACGACACTGACATATAAATATCGATAATAATTTGAAAAAATGGAAGTGGTTGAATGTAACCACATGCGTCAATTTTGTGTCGGTGTTGAACATCGGACACACCTTCAATCAGAAGCGTCAGTGCTACATAGACATATCTCATTTATAGTTTGTATATTTGTGAATTTGATCAGATGTCATGTGTTTATGCTGCTTGTGACTTAAGAGGTCATAGGTCCAAAATCCAAGAAACAACATCTGTTTTATGGGTTTTTGACATGTTTATGTGATTTGTATGTAGATTAACTTCAGTTTTGTTTGTTGTTGTGTTGAAATTTGATGTGTCTGAATGTTGATCTCAATGAATATAGGAGAAAAGCAAACTAGGATATGGTGTTAGGAGAAACAGTTACTAGTTAATTATACTCTCAAGTCTCAATGATCTAGATCTATGAAGCACAAACACGGACACTAGACACATAAACACCGGTAATAATTTGAAAAACAATGGAAGTGATTGAATGTAACCATATCCGTCAATGTGTGTTTGACACCAGCCATGCCTCTAATCAGAAATGTAATTGCTACATACATCCATATCCCGTTTGAAGTCTGTGAATTTGATCAGATGCCATGTATTCATGCTGCTTGTGACTTAGGAAGTCACAGTTTCAAAATCCAAGAAACAACGTTTCTGTTTTTGGGGGTAAAGCTACGTACATTTACCCTCATCAGATTCCACCTGGTGGACGCTATGTGCATTGGGCTTTCCCTCTTCGTGCAGTACTTTAGGAGTGCATAAGTCTTTCTCTAATGTTGAATCTTAGAAGTCAGAAGTAAGGATTCCTTCGATTAATAAAAAGTGGGCGTGGTTTGGTAACTAGATGACCGAAAGCATAAGTGTGTGAATTCTTTGACATATTAGAATGTGAAATTGTTGAGCATGTATTTTTTTTGCCTTTGTGACTTGTAGTTAGCTTTGATACTGTTTGGCAGTTTTCTCGTCGTCTGGCTTTTATGTAATATACCTGTGCTCTGTGTCCAGTCTGTCTATATTGCAAACGTTGAGAATGAGTATGAAACACTAGATCTCTGGCATGTCTACTTTGCATGATTTATTAATAGAACTGGTTGAAACTATCGCAGATTCTCTTTTACCTACTAATCTTGTATAGTCAATTTTCTCATCTAGGTGCATGTAATACATGAATGGGTATGGGCTGGTACTCCGAATGAGGGTGCTGCCTGGCTTCGTTTGCAGAGATTGGGTGATACATGGCATAGAATCCTTTTGATACCTGTAACCGGAGGAGTCATGGTTGGCATGATGTGTGGTTTACTAGAAATATTGGATCAAATAAAGCAGGCAAGTTATTCCCCTCAAAGACAAGGTTTTGACTTGCTTGCAGGAATCTATCCAACGGTAAAGGCTATTCAAGCTGCAGTTACCTTAGGTACTGGCTGTTCATTGGGTCCTGAAGGTCCTAGTGTTGATATCGGAAAGTCGTGCGCCAATGGATTCTCATTAATGATGGAAAACAATAGAGAAAGAAAAATAGCACTTGTTGCAGCTGGTGCAGCAGCTGGAATTTCTTCAGGTAGCTTAGCAATCTTATTGAATCACTATTTAAAACTCATTTTGACATATATTCTAATATTCTGGTCCTTTAAGGCTCCTTGTAACTTTTTAATGATGAAGGCATAAAACTTTTCGTGGCCTTGCACTGCATTGTCAAGCATAGGGCTTGGGTCATTATACCCGCTTGAGTTTGTACTCAAGGGCGGCTTAATGATGATTCCCATTAGATTATTTTAGGTCTAGGCATTCATTGATAAGGTTCTTTTGACTCTTTTTCTTTTCTAAATATGATATTGTTAGGGTTGTTTATGTTTGACTTTTTTGGTTACCTGATTGATGCAATGCTATTGTAATTTGACTGTTAGGGTTTGGAGATTTTTTGGGGTCTATGATTGAGAAATGGCGGTTTTATAGTTTGGGTTGGGCCTAACTCAAACCCTACAAAACTGATATGCAAGGCGATGACTGCTCAAGCTTTATCTACTCGGGCCATATCTCGAGGCAGTGTTAGACTAAACCCATCCTCTGACACCCAACATAATGAACGAGCTGGAGGTTGCAATGAAGGCAACCCAAATGACACTATTAGGAAGCTGGGGGGTGGTGGTGGTGGGGTTGTGGTGTGCACAATTAAGGAGGAATTTCCAAGCATGATACTAAAATTAAAAGTTTTTAACATCTAGAGAAATTATATTCTTTTCTAACTTCAAATCACATCTGCTCTAAACCAAAAGCTAAAAGTGTCACCACCAACTAACTATTTAAGTGAATTTATATAAAAGTGAAGTGCTATATACCTAGAGGTGATTTTCCTAAAATTTTCCATCTTCAAATGCTATTCCCGCATCTTGCAGAATCCTTAAGTTTTAGCCAAATTTATAAACTTTTAAAATCATTCAATATCACAAATCCATCATAAACCCCAAATTTTTTTATAAAAAAAAAATGAGAATTTAATGGGTTTAGGTAAAAGAAAAATAACAATTTGACAACATTTGCTTAAAATTTAATGTAGAGGGACTTCATTGGCTAAAAAAAGATTTGAGAAACCAAAGTCCCTCTTTGTCCAAACTAGAGAGACTAAAAATGTAATTAAGTCTTATTTATTTATAGAGCTTTTGAAAAATTAGCATCTTTTGCTATATAGAAGGTTCCAATGTTGATTAATACCATGAAAATCAAAATTTGTTTGAGTTTAAAAATGCTATATCTGTGAAAATCGAGTCCATGGGCATTTTACTTCCAAGCTCTGGCTTCTAGCTTTACTAGAAAATTACATAAAACTTGTGTTTCCTTTCTGATTTTGGTTGGTTTCGAAGTACGGGTTTATTTTGTGGATTTCTAAATGTTGGTTGTTTTAGCTTTGCATTTTTCTTTCTTCAGGCTTCAATGCAGCAGTTGCAGGCTGTTTTTTCGCTATTGAAACTGTGCTGAGGCCTCTTCGTGCAGAGAATTCTCCTCCATTTACAACTGCCATGATTATATTGGCTTCTGTTATTTCATCAACTGTATCAAATGTCTTACAGGGAACCCAATCAGCTTTTACAGTGCCCGAGTACGAGTTGAAATCTGCTGCTGGTACTAATCTTTAACATTGCTGCACTTTTTGAGTATGGTTCAGGATAGTCTTCATATATGTCTGAATTTGTGACATGCTTTTTCCTTTTTCATCCTTTTTATTTTCTTTGTGAAATCAAATCTGTCTCAAATCCGTCCTACAGTATTGTTATCACTTGAGTAAAACATCTCTATCATTGCTTTTAAGACTCAAGATTTAGGCAAATCTTTCTATCTCCTAAGAATTGAAATTGCACAATCCAGGGATGGTGTAGTAATCTCGTAAGTGAAGCATGCTATAGACATACTTGAGGAAGTAGGTTTGTTAAATGCAAATTCTATTTACACTCGTATAAGTCTTAGTGTCAGACTTCTATAAGATCAGCGGAAGCCAATACCAAAATTCGTAATATTAAGAAGAATGGTTGTAAAACTAAAGTACCTCACATTTATTTGTCCCTTTTGGGATGCTATGTTCTGTATCTTGGAACATGTTAAAAAAGTTCCAGGAAATGGGGTTGAATAATTGTATCTTCGAAGAGAGTTATAGGAAAAGGTGTTGAAAAGATTCTATGATCTGTATTTTTTGAATAATTCATACAACATGGAAACTGTGTGGGTCCATACGAAGATAAAAGAGATACTCAGATTGTTGAATACTCAGATGTCGATTGGGAGTGTTCTCTTGCTAATTAACGATTTACCTCTAGGTATTGTATGTTCATAGGAGGTAACATATTATATTAGAAATTTAAGAAACATAATATGTTGTCTGATATTTCATCATAATGTTGCATGCAGAGCTACCTTTATACCTGATATTAGGAATGCTGTGTGGCGTTGTAAGCGTGGCAATGACTCGTTTGGTTGCTTGGTTCACCAAGTTTTTTGACTATATCCAGGAGAAATTTGGCCTTCCTACTGTAGTCTGCCCTGCCATAGGTGGTTTAGGAGCTGGGATCATTGCCCTTAAATATCCTGGAATATTATACTGGGGTTTCACAAATGTGGAAGAAATTCTACGTACTGGAAAGAGTGCTTCTGCTCCTGGAATATGGCTTCTAACTCAATTAGCAGGTGCTAAGGTTATTGCCACAGCTCTTTGCAAGGGCTCTGGGCTTGTAGGTGGTCTTTACGCACCGAGTTTAATGATTGGTGCTGCAGTTGGTGCTGTGTTTGGAGGCTTTGCTGCAGAAGTTATCAATTCAGCAATTCCTGGAAATGCTGCTGTTGCACAACCCCAGGCTTATGCTCTGGTAAGCCAACTTTACTACCCTTGAAGCATTACGTCGTCAATTGTAGCTCGTGGAAAATAATTGTTTGTTCAAATTCTGGTACACTAATGTGATATAGTGCTGCTGTAGCCACTATTGAACTGCACTTTGTACTAAATCACGTATCACAGAACAGTAGTGGATTGGTAAAATTCTGCTATTCTATAGCGCATTTTAACCACTTTTTGACAACACTGCTGTGAAATATGTCAGTTGTAGGAATGTCACTGTCGACATCGAGGCTGATTTGGTTGATGTAAATTCAGATAGTTAAAAAGTTAATTTAACTTGTGCAGGTTGGAATGGCTGCTACACTAGCATCTGTTTGTTCTGTTCCTTTGACTTCAGTTCTACTTCTGTTTGAGCTCACAAAAGATTATAGAATACTGCTTCCTCTCATGGTAATCAATAAAATTCTCTATTTTTTTTATTTTTTTTGCTAAGTGTTGATTTGTGTATTGTTCCACTGTTCTGCTATTTCATATGAATGACCTGACAAAATGAGTCCTGACATGGTTCCATTGTGTGATTGATGGTTACCATATCTTTTTACTAATGTTAAAGTTTCTTACGTTTAATTAATATTTTCAACTTATATATTGGCAAAATTTCTCAGGGTGCTGTTGGGCTGGCCATTTGGGTTCCCTCAGTGACAAATCAGGGGAAGGAGAGTGATCCACCTGGTAAAAGAAATTTGGCGAGAGGATATTCTTCAGTTTCAAATAATGAAGATGATGATGGGAATGGTTTAGAACTCTCCATTGTTGGAGATGGTTCCGAGCTTGAAGAAATCGATGAAGAACTATTTCTGGACAAACTTAAGGTTGAGTTTGCATTTTTCTTTTTCCTCCTGATCATCGACATCTTCCATCTTTTGTTGTTAAGACTGCTTGTATAAATAGCTTAATTAAGTGCGTATAACATAAGCGCTTATCATATAAATGCTTATGTATTAGCTAATTCTATAACATAATAAAATAAAGTCATTGTTTTATATAACCTATTAGCTATCTGCATAAGCTATCTCGGAGAGCTAATGGAAATAAGCTGAAAACAACTTAAGGACATGTCATTAGCTATTTCCATAAGCTCTCCCAAATACACTCACTTGAGCTTATGTCAATAGATAAGTTCAAATAAGTCAATCCAAACATACCTAATTTCTCAATGTCACTGTTCCTCTTGTCAGGTTTCTCAGGCCATGTCGAAATACTATCTGAAGGTTCCGTCATCTGTAACCCTCAAAGATGCAATCAAAATCATGCATGACAGCCAGCAAAACTGTGTGCTGGTAGTTGATGAAGATGATTTTCTTGAAGGGATATTGACATATGGTGACATTAGAAGGTGCCGATCCGAGAAGTCTAATGAGACTTCTAAGAGCGACTCAGATCCTTTGGATGTATGTAGAAAATGCCCCTGGTCTTTATATATTTGAAGAGTGACGTGTCTGTTTGCAAATTGTTTTGGAAACAGTTAATTCTGAGAAAATGGAACATAGAAAAAAATGTTGAAAGGAAATGTTTTCGACATGCTAATTATTATTAATGGAACTTAAAATTGTCATTAATTTTATCTTAGGAATCTTATTTATACTGTCTATACTTCCTTTTTGCTGTTAGTTTCTTCTAATAATATAATTTATATTCAATATTTGACAGGTGAACACATTGCTTGTTTCCTCTGTTTGTACTCGAGGAATGAGCTATCGTGGACGAGAACGTGGACTTTTAACTTGTTATCCAAATACTAATTTGGCCATGGCCAGAGAGTTGATGGAGGCCAAGGGTGTTAAGCAATTACCGGTAGTTAAACGCGGTGGAAATCACAATAGAGAGAGGAAGCGAAGAATTGTCGGTCTCCTTCATTATGACGAACTATGGCACTGTCTCAGGTTTCCCCTTAAATCTAATACTTACTCTATAGTAAAAGATCTGCTTCCGTAACCAACTTAGTTTCAAGGACTCTCTTTTCAGCTTATAATATCTGCATTTCCTTGAAATTTTCAACCCATTTGTTATGGCTCTTTTAAAAAATATAACCACTTTTAAGAGTTTTTCATCTGTTTGTTACAATGTTTTTTTAAAAAAAAAATGAGAATGATTATCTAAAATATTATTAAAAACCGAGTACAATAGAAGCAGTGTTGAGTAGAAAATCTTACAGTTCTCTATGAAAAAAATTTAAGCATGTAAGGACTTCTTTGTAAGTAAAGAGTCATAAACTTATTTTAATTGAAAAAATCTTGCAAGTATAAAGTTAATCCAAACTGAGCATAATTCTTTCCATTCGTATTACTGTTTTTGGGCATCAATTCTTGGTTCAGGCTTGATGATATTTATGTTAATGTGCAGGAAAGAGATTAACCACAGAAAGTTAGATTATCAAAGTAGAACAGAAAACAATTTGACAAATGGGCATTAA
Protein sequence

>MS.gene56269.t1

MPENDQLRLLGSSEDAERDIEAHGSEHIAGVNGGGGGGSGSGKGLRDMLKLSGHHRHSFKRLERERERERDRDVDNNNNNVRDSSHSEFDLHSFDSSGDVLADSAPPEWALLLLGCLLGLATGLLVAAFNKGVHVIHEWVWAGTPNEGAAWLRLQRLGDTWHRILLIPVTGGVMVGMMCGLLEILDQIKQASYSPQRQGFDLLAGIYPTVKAIQAAVTLGTGCSLGPEGPSVDIGKSCANGFSLMMENNRERKIALVAAGAAAGISSGFNAAVAGCFFAIETVLRPLRAENSPPFTTAMIILASVISSTVSNVLQGTQSAFTVPEYELKSAAELPLYLILGMLCGVVSVAMTRLVAWFTKFFDYIQEKFGLPTVVCPAIGGLGAGIIALKYPGILYWGFTNVEEILRTGKSASAPGIWLLTQLAGAKVIATALCKGSGLVGGLYAPSLMIGAAVGAVFGGFAAEVINSAIPGNAAVAQPQAYALVGMAATLASVCSVPLTSVLLLFELTKDYRILLPLMGAVGLAIWVPSVTNQGKESDPPGKRNLARGYSSVSNNEDDDGNGLELSIVGDGSELEEIDEELFLDKLKVSQAMSKYYLKVPSSVTLKDAIKIMHDSQQNCVLVVDEDDFLEGILTYGDIRRCRSEKSNETSKSDSDPLDVNTLLVSSVCTRGMSYRGRERGLLTCYPNTNLAMARELMEAKGVKQLPVVKRGGNHNRERKRRIVGLLHYDELWHCLRKEINHRKLDYQSRTENNLTNGH