Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56291.t1 | XP_003598311.1 | 95.9 | 121 | 5 | 0 | 7 | 127 | 50 | 170 | 2.30E-62 | 248.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56291.t1 | Q9FKT1 | 65.0 | 120 | 39 | 1 | 11 | 127 | 49 | 168 | 1.9e-41 | 169.9 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56291.t1 | G7J0F7 | 95.9 | 121 | 5 | 0 | 7 | 127 | 50 | 170 | 1.6e-62 | 248.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene051171 | MS.gene56291 | -0.822785 | 1.97E-53 | -1.69E-46 |
| MS.gene051242 | MS.gene56291 | -0.809275 | 2.05E-50 | -1.69E-46 |
| MS.gene051244 | MS.gene56291 | -0.836225 | 1.06E-56 | -1.69E-46 |
| MS.gene051940 | MS.gene56291 | 0.818524 | 1.87E-52 | -1.69E-46 |
| MS.gene052715 | MS.gene56291 | 0.801283 | 9.69E-49 | -1.69E-46 |
| MS.gene056664 | MS.gene56291 | -0.801077 | 1.07E-48 | -1.69E-46 |
| MS.gene057020 | MS.gene56291 | 0.84168 | 4.10E-58 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56291.t1 | MTR_3g010180 | 95.868 | 121 | 5 | 0 | 7 | 127 | 50 | 170 | 7.39e-84 | 243 |
| MS.gene56291.t1 | MTR_6g025620 | 55.556 | 117 | 52 | 0 | 11 | 127 | 37 | 153 | 7.91e-46 | 146 |
| MS.gene56291.t1 | MTR_6g025670 | 69.880 | 83 | 25 | 0 | 10 | 92 | 55 | 137 | 1.77e-40 | 133 |
| MS.gene56291.t1 | MTR_6g018540 | 69.880 | 83 | 25 | 0 | 10 | 92 | 55 | 137 | 1.77e-40 | 133 |
| MS.gene56291.t1 | MTR_6g011010 | 66.279 | 86 | 28 | 1 | 7 | 91 | 44 | 129 | 5.58e-38 | 125 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56291.t1 | AT5G56170 | 65.000 | 120 | 39 | 1 | 11 | 127 | 49 | 168 | 1.12e-53 | 166 |
| MS.gene56291.t1 | AT4G26466 | 52.419 | 124 | 55 | 2 | 7 | 127 | 42 | 164 | 8.04e-43 | 139 |
| MS.gene56291.t1 | AT2G20700 | 56.410 | 117 | 51 | 0 | 11 | 127 | 44 | 160 | 1.29e-42 | 138 |
| MS.gene56291.t1 | AT4G28280 | 57.143 | 119 | 48 | 1 | 7 | 125 | 23 | 138 | 3.74e-42 | 136 |
| MS.gene56291.t1 | AT4G28280 | 57.143 | 119 | 48 | 1 | 7 | 125 | 44 | 159 | 3.76e-42 | 137 |
| MS.gene56291.t1 | AT4G28280 | 56.897 | 116 | 47 | 1 | 10 | 125 | 21 | 133 | 1.79e-41 | 134 |
Find 31 sgRNAs with CRISPR-Local
Find 31 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| TTTAGCTACATTAATCTTTA+TGG | 0.084552 | 3.1:-5239505 | MS.gene56291:CDS |
| TCATTTATCACATCAGTATA+AGG | 0.287486 | 3.1:+5239556 | None:intergenic |
| AAATGCTCCACAACATTCCT+TGG | 0.377041 | 3.1:+5239594 | None:intergenic |
| CATTCCTTGGGAGGGTACTT+AGG | 0.429542 | 3.1:+5239607 | None:intergenic |
| AATGCTCCACAACATTCCTT+GGG | 0.478717 | 3.1:+5239595 | None:intergenic |
| CATTTATCACATCAGTATAA+GGG | 0.483862 | 3.1:+5239557 | None:intergenic |
| CACTCGCTCGCAAAGAGGCC+AGG | 0.497941 | 3.1:+5239472 | None:intergenic |
| TTGTTTCACATTGCACCTGC+AGG | 0.503151 | 3.1:-5239688 | MS.gene56291:CDS |
| CACGACACTCGCTCGCAAAG+AGG | 0.542743 | 3.1:+5239467 | None:intergenic |
| ACATACTCCATCTCTAGTGC+TGG | 0.553326 | 3.1:-5239371 | MS.gene56291:CDS |
| TATTAAAATTAAGAAGATGC+AGG | 0.556222 | 3.1:+5239339 | None:intergenic |
| CGCTCGCAAAGAGGCCAGGA+GGG | 0.563412 | 3.1:+5239476 | None:intergenic |
| GAGCGAGTGTCGTGAAGGGA+AGG | 0.564311 | 3.1:-5239458 | MS.gene56291:CDS |
| GATTTGATTTGCAGTATCAT+CGG | 0.571086 | 3.1:+5239396 | None:intergenic |
| AAATTCACAGAACAGCCTGC+AGG | 0.577799 | 3.1:+5239673 | None:intergenic |
| GTGAGCACCAGCACTAGAGA+TGG | 0.582002 | 3.1:+5239364 | None:intergenic |
| GTATCATCGGCGGACACTGA+TGG | 0.584167 | 3.1:+5239409 | None:intergenic |
| CTTTATGGAAGATACCCTCC+TGG | 0.585291 | 3.1:-5239490 | MS.gene56291:CDS |
| TACCCTCCCAAGGAATGTTG+TGG | 0.591187 | 3.1:-5239601 | MS.gene56291:CDS |
| TTTGCGAGCGAGTGTCGTGA+AGG | 0.593755 | 3.1:-5239463 | MS.gene56291:CDS |
| CTCCACAACATTCCTTGGGA+GGG | 0.609435 | 3.1:+5239599 | None:intergenic |
| TTGCGAGCGAGTGTCGTGAA+GGG | 0.616291 | 3.1:-5239462 | MS.gene56291:CDS |
| GCTCCACAACATTCCTTGGG+AGG | 0.623412 | 3.1:+5239598 | None:intergenic |
| GAGTGTCGTGAAGGGAAGGA+AGG | 0.628790 | 3.1:-5239454 | MS.gene56291:CDS |
| TCGCTCGCAAAGAGGCCAGG+AGG | 0.631835 | 3.1:+5239475 | None:intergenic |
| AAGATTAATGTAGCTAAACA+TGG | 0.635506 | 3.1:+5239510 | None:intergenic |
| ATAATCACAAGCAAATGCAA+AGG | 0.641999 | 3.1:-5239631 | MS.gene56291:CDS |
| AATGTAGCTAAACATGGTTG+AGG | 0.646232 | 3.1:+5239516 | None:intergenic |
| TCATCGGCGGACACTGATGG+TGG | 0.646590 | 3.1:+5239412 | None:intergenic |
| AGGACCTAAGTACCCTCCCA+AGG | 0.651427 | 3.1:-5239611 | MS.gene56291:CDS |
| TTGATTTGCAGTATCATCGG+CGG | 0.696584 | 3.1:+5239399 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTAAAATTAAGAAGATGC+AGG | + | chr3.1:5239691-5239710 | None:intergenic | 20.0% |
| !! | TTTAGCTACATTAATCTTTA+TGG | - | chr3.1:5239522-5239541 | MS.gene56291:CDS | 20.0% |
| ! | AAGATTAATGTAGCTAAACA+TGG | + | chr3.1:5239520-5239539 | None:intergenic | 25.0% |
| ! | CATTTATCACATCAGTATAA+GGG | + | chr3.1:5239473-5239492 | None:intergenic | 25.0% |
| ! | TCATTTATCACATCAGTATA+AGG | + | chr3.1:5239474-5239493 | None:intergenic | 25.0% |
| ATAATCACAAGCAAATGCAA+AGG | - | chr3.1:5239396-5239415 | MS.gene56291:CDS | 30.0% | |
| !! | GATTTGATTTGCAGTATCAT+CGG | + | chr3.1:5239634-5239653 | None:intergenic | 30.0% |
| AATGTAGCTAAACATGGTTG+AGG | + | chr3.1:5239514-5239533 | None:intergenic | 35.0% | |
| AAATGCTCCACAACATTCCT+TGG | + | chr3.1:5239436-5239455 | None:intergenic | 40.0% | |
| AATGCTCCACAACATTCCTT+GGG | + | chr3.1:5239435-5239454 | None:intergenic | 40.0% | |
| !! | TTGATTTGCAGTATCATCGG+CGG | + | chr3.1:5239631-5239650 | None:intergenic | 40.0% |
| AAATTCACAGAACAGCCTGC+AGG | + | chr3.1:5239357-5239376 | None:intergenic | 45.0% | |
| ACATACTCCATCTCTAGTGC+TGG | - | chr3.1:5239656-5239675 | MS.gene56291:CDS | 45.0% | |
| CTTTATGGAAGATACCCTCC+TGG | - | chr3.1:5239537-5239556 | MS.gene56291:CDS | 45.0% | |
| TTGTTTCACATTGCACCTGC+AGG | - | chr3.1:5239339-5239358 | MS.gene56291:CDS | 45.0% | |
| CTCCACAACATTCCTTGGGA+GGG | + | chr3.1:5239431-5239450 | None:intergenic | 50.0% | |
| TACCCTCCCAAGGAATGTTG+TGG | - | chr3.1:5239426-5239445 | MS.gene56291:CDS | 50.0% | |
| !! | CATTCCTTGGGAGGGTACTT+AGG | + | chr3.1:5239423-5239442 | None:intergenic | 50.0% |
| AGGACCTAAGTACCCTCCCA+AGG | - | chr3.1:5239416-5239435 | MS.gene56291:CDS | 55.0% | |
| GCTCCACAACATTCCTTGGG+AGG | + | chr3.1:5239432-5239451 | None:intergenic | 55.0% | |
| GTATCATCGGCGGACACTGA+TGG | + | chr3.1:5239621-5239640 | None:intergenic | 55.0% | |
| GTGAGCACCAGCACTAGAGA+TGG | + | chr3.1:5239666-5239685 | None:intergenic | 55.0% | |
| ! | GAGTGTCGTGAAGGGAAGGA+AGG | - | chr3.1:5239573-5239592 | MS.gene56291:CDS | 55.0% |
| ! | TTGCGAGCGAGTGTCGTGAA+GGG | - | chr3.1:5239565-5239584 | MS.gene56291:CDS | 55.0% |
| ! | TTTGCGAGCGAGTGTCGTGA+AGG | - | chr3.1:5239564-5239583 | MS.gene56291:CDS | 55.0% |
| CACGACACTCGCTCGCAAAG+AGG | + | chr3.1:5239563-5239582 | None:intergenic | 60.0% | |
| TCATCGGCGGACACTGATGG+TGG | + | chr3.1:5239618-5239637 | None:intergenic | 60.0% | |
| ! | GAGCGAGTGTCGTGAAGGGA+AGG | - | chr3.1:5239569-5239588 | MS.gene56291:CDS | 60.0% |
| CACTCGCTCGCAAAGAGGCC+AGG | + | chr3.1:5239558-5239577 | None:intergenic | 65.0% | |
| CGCTCGCAAAGAGGCCAGGA+GGG | + | chr3.1:5239554-5239573 | None:intergenic | 65.0% | |
| TCGCTCGCAAAGAGGCCAGG+AGG | + | chr3.1:5239555-5239574 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr3.1 | gene | 5239333 | 5239716 | 5239333 | ID=MS.gene56291 |
| chr3.1 | mRNA | 5239333 | 5239716 | 5239333 | ID=MS.gene56291.t1;Parent=MS.gene56291 |
| chr3.1 | exon | 5239333 | 5239716 | 5239333 | ID=MS.gene56291.t1.exon1;Parent=MS.gene56291.t1 |
| chr3.1 | CDS | 5239333 | 5239716 | 5239333 | ID=cds.MS.gene56291.t1;Parent=MS.gene56291.t1 |
| Gene Sequence |
| Protein sequence |
