Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56937.t1 | RHN53768.1 | 99.2 | 118 | 1 | 0 | 9 | 126 | 23 | 140 | 8.50E-49 | 203.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56937.t1 | F4JP48 | 72.9 | 118 | 32 | 0 | 9 | 126 | 23 | 140 | 1.2e-40 | 167.5 |
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56937.t1 | G7JXD8 | 99.2 | 118 | 1 | 0 | 9 | 126 | 23 | 140 | 6.1e-49 | 203.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059919 | MS.gene56937 | 0.811908 | 5.53E-51 | -1.69E-46 |
| MS.gene059920 | MS.gene56937 | 0.812816 | 3.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56937.t1 | MTR_5g012900 | 99.153 | 118 | 1 | 0 | 9 | 126 | 23 | 140 | 4.70e-77 | 239 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene56937.t1 | AT4G17380 | 78.814 | 118 | 25 | 0 | 9 | 126 | 23 | 140 | 3.86e-58 | 193 |
| MS.gene56937.t1 | AT4G17380 | 78.814 | 118 | 25 | 0 | 9 | 126 | 23 | 140 | 4.66e-58 | 194 |
Find 30 sgRNAs with CRISPR-Local
Find 106 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| GGAAACTTATTAACAACATT+TGG | 0.312904 | 8.1:-18759891 | MS.gene56937:CDS |
| TACAAGTACAGTTACTGAAT+TGG | 0.374329 | 8.1:-18761221 | MS.gene56937:CDS |
| TAATTGAAATGCAGGTCATA+TGG | 0.387079 | 8.1:-18759912 | MS.gene56937:intron |
| GGATCCTTCAGCACTTGGTT+TGG | 0.396208 | 8.1:-18760879 | MS.gene56937:CDS |
| GAACTGGAGGCTGACTTGTT+TGG | 0.401346 | 8.1:+18761244 | None:intergenic |
| GAAGGAATTTGAATGGATAA+TGG | 0.412903 | 8.1:-18761749 | MS.gene56937:intron |
| AGTAACTGTACTTGTAGAAC+TGG | 0.447228 | 8.1:+18761228 | None:intergenic |
| AAGGAATTTCAAGGAAATTG+AGG | 0.455849 | 8.1:-18760087 | MS.gene56937:intron |
| GCTAAGGATCCTTCAGCACT+TGG | 0.462191 | 8.1:-18760884 | MS.gene56937:CDS |
| TACTGTTGGAAGGAATTTCA+AGG | 0.470034 | 8.1:-18760096 | MS.gene56937:CDS |
| TTTCTGACAGGTTGGATTAG+CGG | 0.490064 | 8.1:-18761383 | MS.gene56937:intron |
| CAGCTATGAAGGAATTTGAA+TGG | 0.494718 | 8.1:-18761756 | None:intergenic |
| AACTTATTAACAACATTTGG+AGG | 0.499331 | 8.1:-18759888 | MS.gene56937:CDS |
| CTGAAGGATCCTTAGCGGCT+AGG | 0.499881 | 8.1:+18760891 | None:intergenic |
| AACTATGCTGCTGATACTGT+TGG | 0.502229 | 8.1:-18760110 | MS.gene56937:CDS |
| CTGGAGGCTGACTTGTTTGG+AGG | 0.509425 | 8.1:+18761247 | None:intergenic |
| GGAGGAACGATGATCACAAT+GGG | 0.535429 | 8.1:+18761265 | None:intergenic |
| TGGTTTCAATATATTGTGAA+AGG | 0.536588 | 8.1:+18761332 | None:intergenic |
| CAAGCAGTATTGTCTGTGCT+TGG | 0.551221 | 8.1:-18760846 | MS.gene56937:CDS |
| GTGTCCAAACCAAGTGCTGA+AGG | 0.553150 | 8.1:+18760875 | None:intergenic |
| CTGCTGCCGCTACTTTAAAG+TGG | 0.564097 | 8.1:-18760820 | MS.gene56937:intron |
| AAGTGCTGAAGGATCCTTAG+CGG | 0.579479 | 8.1:+18760886 | None:intergenic |
| TGTGTCATCAAAACAACCAC+GGG | 0.597523 | 8.1:+18761038 | None:intergenic |
| AACTGTACTTGTAGAACTGG+AGG | 0.621730 | 8.1:+18761231 | None:intergenic |
| GCAGCATAGTTCTTACGCAG+TGG | 0.627252 | 8.1:+18760122 | None:intergenic |
| TGGAGGAACGATGATCACAA+TGG | 0.638070 | 8.1:+18761264 | None:intergenic |
| GTAGTACCACTTTAAAGTAG+CGG | 0.642068 | 8.1:+18760814 | None:intergenic |
| ATGCTGCTGATACTGTTGGA+AGG | 0.648055 | 8.1:-18760106 | MS.gene56937:CDS |
| TTGTGTCATCAAAACAACCA+CGG | 0.656729 | 8.1:+18761037 | None:intergenic |
| ATGCAGGTAGTGTTGGCCCG+TGG | 0.678245 | 8.1:-18761054 | MS.gene56937:intron |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | ATATTTAGCATCTTTAAATA+AGG | + | chr8.1:18761580-18761599 | None:intergenic | 15.0% |
| !! | TAATTGATATACATTACAAA+GGG | - | chr8.1:18759994-18760013 | MS.gene56937:intron | 15.0% |
| !!! | TTATATTTTAATTGAAATGC+AGG | - | chr8.1:18761689-18761708 | MS.gene56937:intron | 15.0% |
| !! | AAAATAATATATGGTTGGAA+AGG | + | chr8.1:18761153-18761172 | None:intergenic | 20.0% |
| !! | AATTGATATACATTACAAAG+GGG | - | chr8.1:18759995-18760014 | MS.gene56937:intron | 20.0% |
| !! | AATTGATATACCAATACATA+AGG | - | chr8.1:18761007-18761026 | MS.gene56937:intron | 20.0% |
| !! | ATCAAATTCTTTGTTAACAT+GGG | + | chr8.1:18760113-18760132 | None:intergenic | 20.0% |
| !! | CAATCAAAATAATATATGGT+TGG | + | chr8.1:18761158-18761177 | None:intergenic | 20.0% |
| !! | CCAACAATCAAAATAATATA+TGG | + | chr8.1:18761162-18761181 | None:intergenic | 20.0% |
| !! | CTAATTGATATACATTACAA+AGG | - | chr8.1:18759993-18760012 | MS.gene56937:intron | 20.0% |
| !! | TATCAAATTCTTTGTTAACA+TGG | + | chr8.1:18760114-18760133 | None:intergenic | 20.0% |
| !! | TTGTAATGTATATCAATTAG+TGG | + | chr8.1:18759993-18760012 | None:intergenic | 20.0% |
| !!! | CATTTTTTTTATGACTTCTA+TGG | - | chr8.1:18760135-18760154 | MS.gene56937:CDS | 20.0% |
| !!! | CCATATATTATTTTGATTGT+TGG | - | chr8.1:18761159-18761178 | MS.gene56937:intron | 20.0% |
| !!! | TTGTTTGTTATTTCAAGTTT+AGG | + | chr8.1:18759934-18759953 | None:intergenic | 20.0% |
| !!! | TTTCATTTCAATTGATTTCA+AGG | - | chr8.1:18760619-18760638 | MS.gene56937:intron | 20.0% |
| ! | AACTTATTAACAACATTTGG+AGG | - | chr8.1:18761721-18761740 | MS.gene56937:intron | 25.0% |
| ! | CAAAATTTAGTGCATTTATG+AGG | + | chr8.1:18760670-18760689 | None:intergenic | 25.0% |
| ! | CAAGTAGTTAATACTCTTAT+TGG | - | chr8.1:18761043-18761062 | MS.gene56937:CDS | 25.0% |
| ! | CCAATAATATCCTTATGTAT+TGG | + | chr8.1:18761020-18761039 | None:intergenic | 25.0% |
| ! | CCAATACATAAGGATATTAT+TGG | - | chr8.1:18761017-18761036 | MS.gene56937:intron | 25.0% |
| ! | GGAAACTTATTAACAACATT+TGG | - | chr8.1:18761718-18761737 | MS.gene56937:intron | 25.0% |
| ! | TCAAAAACATAACCATTAGA+GGG | + | chr8.1:18760474-18760493 | None:intergenic | 25.0% |
| ! | TCAATTTATTTGTCCTTCTA+CGG | - | chr8.1:18760825-18760844 | MS.gene56937:CDS | 25.0% |
| ! | TGAATTTATATTGCCTAACT+TGG | - | chr8.1:18760187-18760206 | MS.gene56937:intron | 25.0% |
| ! | TGGTTTCAATATATTGTGAA+AGG | + | chr8.1:18760280-18760299 | None:intergenic | 25.0% |
| ! | TTATCACCTATAGATTTCAA+AGG | + | chr8.1:18761089-18761108 | None:intergenic | 25.0% |
| ! | TTCTTATTGTAGTAGAAGTT+TGG | - | chr8.1:18760959-18760978 | MS.gene56937:intron | 25.0% |
| !! | AATCAATTGAATTTTCTGAC+AGG | - | chr8.1:18760214-18760233 | MS.gene56937:intron | 25.0% |
| !! | ATTAATTACACTCTTTTCGA+AGG | - | chr8.1:18760873-18760892 | MS.gene56937:CDS | 25.0% |
| !! | TTCAATTGATTTCAAGGTTT+AGG | - | chr8.1:18760625-18760644 | MS.gene56937:intron | 25.0% |
| !! | TTTTATAACACCAAAACTGA+TGG | - | chr8.1:18761401-18761420 | MS.gene56937:intron | 25.0% |
| !!! | ATATTATTTTGATTGTTGGC+AGG | - | chr8.1:18761163-18761182 | MS.gene56937:intron | 25.0% |
| !!! | CAGTTTTGGTGTTATAAAAA+TGG | + | chr8.1:18761400-18761419 | None:intergenic | 25.0% |
| AAAGTTCATACATGTTCGAA+AGG | - | chr8.1:18760052-18760071 | MS.gene56937:intron | 30.0% | |
| AAGGAATTTCAAGGAAATTG+AGG | - | chr8.1:18761522-18761541 | MS.gene56937:intron | 30.0% | |
| CTCAAAAACATAACCATTAG+AGG | + | chr8.1:18760475-18760494 | None:intergenic | 30.0% | |
| CTTCTATGGTATTAAAGAAC+TGG | - | chr8.1:18760149-18760168 | MS.gene56937:intron | 30.0% | |
| GAAGGAATTTGAATGGATAA+TGG | - | chr8.1:18759860-18759879 | MS.gene56937:CDS | 30.0% | |
| GTTAAGACTTACCTATATTG+AGG | - | chr8.1:18761325-18761344 | MS.gene56937:CDS | 30.0% | |
| TAACCATAGAACCTCAATAT+AGG | + | chr8.1:18761339-18761358 | None:intergenic | 30.0% | |
| TAATTGAAATGCAGGTCATA+TGG | - | chr8.1:18761697-18761716 | MS.gene56937:intron | 30.0% | |
| TACAAGTACAGTTACTGAAT+TGG | - | chr8.1:18760388-18760407 | MS.gene56937:intron | 30.0% | |
| TCTATAGGTGATAAATCTTC+AGG | - | chr8.1:18761095-18761114 | MS.gene56937:intron | 30.0% | |
| TGGATCAAATTCAAAATGTC+TGG | + | chr8.1:18761436-18761455 | None:intergenic | 30.0% | |
| TTACCTATATTGAGGTTCTA+TGG | - | chr8.1:18761333-18761352 | MS.gene56937:CDS | 30.0% | |
| TTCAAGGAAATTGAGGTTAT+TGG | - | chr8.1:18761529-18761548 | MS.gene56937:intron | 30.0% | |
| ! | AATTGAATTTTCTGACAGGT+TGG | - | chr8.1:18760218-18760237 | MS.gene56937:intron | 30.0% |
| ! | GAATTGGTTGACAGATTTTA+TGG | - | chr8.1:18760404-18760423 | MS.gene56937:intron | 30.0% |
| !! | CAATTGATTTGATCCAAGTT+AGG | + | chr8.1:18760203-18760222 | None:intergenic | 30.0% |
| !! | CATTGTCCTTTGAAATCTAT+AGG | - | chr8.1:18761080-18761099 | MS.gene56937:intron | 30.0% |
| !! | CTAATAGTGGCTTTAATAGT+TGG | + | chr8.1:18761229-18761248 | None:intergenic | 30.0% |
| !! | GGATAATGGTATTCATTTTG+AGG | - | chr8.1:18759874-18759893 | MS.gene56937:CDS | 30.0% |
| !!! | AGGATTTTAATTGTTGTAGC+AGG | + | chr8.1:18761133-18761152 | None:intergenic | 30.0% |
| !!! | GTTATTGGTTTGTCAAGTTT+TGG | - | chr8.1:18761544-18761563 | MS.gene56937:intron | 30.0% |
| !!! | TGTGTTTTGATATGAACTAC+TGG | + | chr8.1:18760300-18760319 | None:intergenic | 30.0% |
| !!! | TTTCGAGTTCTTTTATATGC+AGG | - | chr8.1:18760539-18760558 | MS.gene56937:intron | 30.0% |
| AATAAAAAACCTAGCCGCTA+AGG | - | chr8.1:18760709-18760728 | MS.gene56937:intron | 35.0% | |
| AGTAACTGTACTTGTAGAAC+TGG | + | chr8.1:18760384-18760403 | None:intergenic | 35.0% | |
| ATAACACCAAAACTGATGGT+TGG | - | chr8.1:18761405-18761424 | MS.gene56937:intron | 35.0% | |
| ATAACCATTAGAGGGATGTA+AGG | + | chr8.1:18760466-18760485 | None:intergenic | 35.0% | |
| ATACATGCACAACCATGAAT+GGG | + | chr8.1:18761290-18761309 | None:intergenic | 35.0% | |
| CTCATGAAATCAGCTAATAG+TGG | + | chr8.1:18761242-18761261 | None:intergenic | 35.0% | |
| GGACAAATAAATTGAGACAG+AGG | + | chr8.1:18760820-18760839 | None:intergenic | 35.0% | |
| TACTGTTGGAAGGAATTTCA+AGG | - | chr8.1:18761513-18761532 | MS.gene56937:intron | 35.0% | |
| TATACATGCACAACCATGAA+TGG | + | chr8.1:18761291-18761310 | None:intergenic | 35.0% | |
| TCCAGAAAACACTACATGTT+AGG | - | chr8.1:18761452-18761471 | MS.gene56937:intron | 35.0% | |
| TTGTGTCATCAAAACAACCA+CGG | + | chr8.1:18760575-18760594 | None:intergenic | 35.0% | |
| ! | CACTATTAGCTGATTTCATG+AGG | - | chr8.1:18761240-18761259 | MS.gene56937:CDS | 35.0% |
| ! | CTTTTATATGCAGGTAGTGT+TGG | - | chr8.1:18760548-18760567 | MS.gene56937:intron | 35.0% |
| ! | GCACTAAATTTTGTGCTTCA+GGG | - | chr8.1:18760677-18760696 | MS.gene56937:intron | 35.0% |
| ! | GGTTGACAGATTTTATGGTT+CGG | - | chr8.1:18760409-18760428 | MS.gene56937:intron | 35.0% |
| ! | GTTCTATGGTTAGTCATTGT+TGG | - | chr8.1:18761347-18761366 | MS.gene56937:CDS | 35.0% |
| ! | TGCACTAAATTTTGTGCTTC+AGG | - | chr8.1:18760676-18760695 | MS.gene56937:intron | 35.0% |
| !! | GTAGTACCACTTTAAAGTAG+CGG | + | chr8.1:18760798-18760817 | None:intergenic | 35.0% |
| !!! | ATTTTATGGTTCGGTCAAGA+AGG | - | chr8.1:18760418-18760437 | MS.gene56937:intron | 35.0% |
| !!! | TGGTTGTTTTGATGACACAA+AGG | - | chr8.1:18760575-18760594 | MS.gene56937:intron | 35.0% |
| AACTATGCTGCTGATACTGT+TGG | - | chr8.1:18761499-18761518 | MS.gene56937:intron | 40.0% | |
| AACTGTACTTGTAGAACTGG+AGG | + | chr8.1:18760381-18760400 | None:intergenic | 40.0% | |
| CATGAGGTAGAAACAGAAGT+AGG | - | chr8.1:18761256-18761275 | MS.gene56937:CDS | 40.0% | |
| GTCTTCAACAACGTTGTAAC+AGG | + | chr8.1:18761202-18761221 | None:intergenic | 40.0% | |
| TAGGATATGATGCCCATTCA+TGG | - | chr8.1:18761275-18761294 | MS.gene56937:CDS | 40.0% | |
| TGTGTCATCAAAACAACCAC+GGG | + | chr8.1:18760574-18760593 | None:intergenic | 40.0% | |
| TTGTCCTTACATCCCTCTAA+TGG | - | chr8.1:18760459-18760478 | MS.gene56937:intron | 40.0% | |
| ! | GCCTAACATGTAGTGTTTTC+TGG | + | chr8.1:18761456-18761475 | None:intergenic | 40.0% |
| ! | TTTCTGACAGGTTGGATTAG+CGG | - | chr8.1:18760226-18760245 | MS.gene56937:intron | 40.0% |
| !!! | GATTAGCGGCTTTTGATTTG+AGG | - | chr8.1:18760240-18760259 | MS.gene56937:intron | 40.0% |
| !!! | TTGGTTTGTCAAGTTTTGGC+AGG | - | chr8.1:18761548-18761567 | MS.gene56937:intron | 40.0% |
| ATGCTGCTGATACTGTTGGA+AGG | - | chr8.1:18761503-18761522 | MS.gene56937:intron | 45.0% | |
| CAAGCAGTATTGTCTGTGCT+TGG | - | chr8.1:18760763-18760782 | MS.gene56937:intron | 45.0% | |
| CACTCGACTGAATTCCTAAC+AGG | + | chr8.1:18760027-18760046 | None:intergenic | 45.0% | |
| GACATGTGCATTACCGTAGA+AGG | + | chr8.1:18760841-18760860 | None:intergenic | 45.0% | |
| GGAGGAACGATGATCACAAT+GGG | + | chr8.1:18760347-18760366 | None:intergenic | 45.0% | |
| TGGAGGAACGATGATCACAA+TGG | + | chr8.1:18760348-18760367 | None:intergenic | 45.0% | |
| ! | GACACACCAACCATCAGTTT+TGG | + | chr8.1:18761414-18761433 | None:intergenic | 45.0% |
| !! | AAGTGCTGAAGGATCCTTAG+CGG | + | chr8.1:18760726-18760745 | None:intergenic | 45.0% |
| GCAGCATAGTTCTTACGCAG+TGG | + | chr8.1:18761490-18761509 | None:intergenic | 50.0% | |
| GCTAAGGATCCTTCAGCACT+TGG | - | chr8.1:18760725-18760744 | MS.gene56937:intron | 50.0% | |
| GGATCCTTCAGCACTTGGTT+TGG | - | chr8.1:18760730-18760749 | MS.gene56937:intron | 50.0% | |
| ! | CAAAGGGGCTTTTGCCTGTT+AGG | - | chr8.1:18760010-18760029 | MS.gene56937:intron | 50.0% |
| ! | CTGCTGCCGCTACTTTAAAG+TGG | - | chr8.1:18760789-18760808 | MS.gene56937:intron | 50.0% |
| !! | GAACTGGAGGCTGACTTGTT+TGG | + | chr8.1:18760368-18760387 | None:intergenic | 50.0% |
| !! | GTGTCCAAACCAAGTGCTGA+AGG | + | chr8.1:18760737-18760756 | None:intergenic | 50.0% |
| CTGAAGGATCCTTAGCGGCT+AGG | + | chr8.1:18760721-18760740 | None:intergenic | 55.0% | |
| !! | CTGGAGGCTGACTTGTTTGG+AGG | + | chr8.1:18760365-18760384 | None:intergenic | 55.0% |
| !! | ATGCAGGTAGTGTTGGCCCG+TGG | - | chr8.1:18760555-18760574 | MS.gene56937:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 18759858 | 18761773 | 18759858 | ID=MS.gene56937 |
| chr8.1 | mRNA | 18759858 | 18761773 | 18759858 | ID=MS.gene56937.t1;Parent=MS.gene56937 |
| chr8.1 | exon | 18761750 | 18761773 | 18761750 | ID=MS.gene56937.t1.exon1;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18761750 | 18761773 | 18761750 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| chr8.1 | exon | 18761192 | 18761395 | 18761192 | ID=MS.gene56937.t1.exon2;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18761192 | 18761395 | 18761192 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| chr8.1 | exon | 18761035 | 18761070 | 18761035 | ID=MS.gene56937.t1.exon3;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18761035 | 18761070 | 18761035 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| chr8.1 | exon | 18760821 | 18760933 | 18760821 | ID=MS.gene56937.t1.exon4;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18760821 | 18760933 | 18760821 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| chr8.1 | exon | 18760088 | 18760157 | 18760088 | ID=MS.gene56937.t1.exon5;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18760088 | 18760157 | 18760088 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| chr8.1 | exon | 18759858 | 18759920 | 18759858 | ID=MS.gene56937.t1.exon6;Parent=MS.gene56937.t1 |
| chr8.1 | CDS | 18759858 | 18759920 | 18759858 | ID=cds.MS.gene56937.t1;Parent=MS.gene56937.t1 |
| Gene Sequence |
| Protein sequence |
