Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059920.t1 | GAU25724.1 | 62.3 | 61 | 22 | 1 | 85 | 144 | 54 | 114 | 1.30E-09 | 73.2 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059920.t1 | A0A2Z6LZX5 | 62.3 | 61 | 22 | 1 | 85 | 144 | 54 | 114 | 9.1e-10 | 73.2 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene059919 | MS.gene059920 | 0.845078 | 5.08E-59 | -1.69E-46 |
| MS.gene059920 | MS.gene56843 | 0.816788 | 4.62E-52 | -1.69E-46 |
| MS.gene059920 | MS.gene56890 | 0.852325 | 4.97E-61 | -1.69E-46 |
| MS.gene059920 | MS.gene56896 | 0.800156 | 1.65E-48 | -1.69E-46 |
| MS.gene059920 | MS.gene56937 | 0.812816 | 3.50E-51 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059920.t1 | MTR_5g069640 | 60.000 | 60 | 23 | 1 | 85 | 143 | 54 | 113 | 3.30e-17 | 74.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene059920.t1 | AT2G29590 | 47.619 | 63 | 32 | 1 | 85 | 146 | 51 | 113 | 8.86e-12 | 60.1 |
Find 36 sgRNAs with CRISPR-Local
Find 90 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AAGATAAAAGATGACGATTA+TGG | 0.352437 | 8.1:+19426138 | None:intergenic |
| ATCCAAGCACGCAAGGTTCC+AGG | 0.364500 | 8.1:-19426104 | MS.gene059920:CDS |
| ACCCTGGAACCTTGCGTGCT+TGG | 0.365081 | 8.1:+19426102 | None:intergenic |
| CTCTACTTCGTGTATATAAC+GGG | 0.432324 | 8.1:+19424424 | None:intergenic |
| TCACCGTCACGCTGTACAAT+TGG | 0.434772 | 8.1:-19425552 | MS.gene059920:CDS |
| CATATCAACATAAACATTCA+TGG | 0.435825 | 8.1:+19424761 | None:intergenic |
| AACATGAGCAGTTGCAAGAA+AGG | 0.448909 | 8.1:+19424731 | None:intergenic |
| GGGTCTGTAATCCATGGAAA+TGG | 0.479799 | 8.1:-19424789 | MS.gene059920:CDS |
| TCAGAGCCACAATCGATTCA+AGG | 0.485541 | 8.1:-19426198 | MS.gene059920:CDS |
| ACCCTCGCAACCGTGTTTGA+AGG | 0.487449 | 8.1:-19426239 | MS.gene059920:CDS |
| AACACTGGAAAGTTGGCTAA+TGG | 0.488900 | 8.1:-19424843 | MS.gene059920:CDS |
| ACAGGACAACACTGGAAAGT+TGG | 0.504393 | 8.1:-19424850 | MS.gene059920:intron |
| TTACCTTGAGAAAGTGAAGT+AGG | 0.510050 | 8.1:+19425498 | None:intergenic |
| TGAATCGATTGTGGCTCTGA+AGG | 0.513017 | 8.1:+19426201 | None:intergenic |
| ACCTTCAAACACGGTTGCGA+GGG | 0.521164 | 8.1:+19426238 | None:intergenic |
| TCATGGGGAGACCATTTCCA+TGG | 0.527491 | 8.1:+19424778 | None:intergenic |
| GCTCTACTTCGTGTATATAA+CGG | 0.540479 | 8.1:+19424423 | None:intergenic |
| TCTCCTACTTCACTTTCTCA+AGG | 0.544227 | 8.1:-19425501 | MS.gene059920:intron |
| GTTGAAGGGTCTGTAATCCA+TGG | 0.545311 | 8.1:-19424795 | MS.gene059920:CDS |
| ATATCAACATAAACATTCAT+GGG | 0.545871 | 8.1:+19424762 | None:intergenic |
| GAGGGTTTGTGAGATTGTCA+TGG | 0.555008 | 8.1:+19426256 | None:intergenic |
| GACCTTCAAACACGGTTGCG+AGG | 0.557313 | 8.1:+19426237 | None:intergenic |
| CTTCTCTTACAGGACAACAC+TGG | 0.558715 | 8.1:-19424858 | MS.gene059920:intron |
| AGGGTTTGTGAGATTGTCAT+GGG | 0.564217 | 8.1:+19426257 | None:intergenic |
| GAGTTGTAGCTTCAGTCTGT+AGG | 0.578701 | 8.1:+19424387 | None:intergenic |
| TCCAAGCACGCAAGGTTCCA+GGG | 0.579520 | 8.1:-19426103 | MS.gene059920:CDS |
| TGTTGACCTTGAATCGATTG+TGG | 0.581091 | 8.1:+19426192 | None:intergenic |
| AGAACAACGGCAAGATCCGA+TGG | 0.604038 | 8.1:+19425523 | None:intergenic |
| TCTCGCAATCCAAGCACGCA+AGG | 0.609643 | 8.1:-19426111 | MS.gene059920:CDS |
| TTGCAACAACTCATCAGCTA+CGG | 0.615568 | 8.1:-19426079 | MS.gene059920:CDS |
| TGATGAGTTGTTGCAAACCC+TGG | 0.631021 | 8.1:+19426086 | None:intergenic |
| TTGCGTGCTTGGATTGCGAG+AGG | 0.634447 | 8.1:+19426113 | None:intergenic |
| AAGCCAATTGTACAGCGTGA+CGG | 0.643220 | 8.1:+19425549 | None:intergenic |
| GAGATTGAGACCTTCAAACA+CGG | 0.645728 | 8.1:+19426229 | None:intergenic |
| TATCAACATAAACATTCATG+GGG | 0.688765 | 8.1:+19424763 | None:intergenic |
| AGTGAAGTAGGAGAGAACAA+CGG | 0.740551 | 8.1:+19425510 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | TATTTATACAAATATCAAAT+AGG | + | chr8.1:19425444-19425463 | None:intergenic | 10.0% |
| !! | AATAATCCTTTATTAGTTAA+GGG | - | chr8.1:19425509-19425528 | MS.gene059920:CDS | 15.0% |
| !! | AGATTAAATTTAAGAAAAAC+TGG | - | chr8.1:19425566-19425585 | MS.gene059920:intron | 15.0% |
| !! | TAATAATCCTTTATTAGTTA+AGG | - | chr8.1:19425508-19425527 | MS.gene059920:CDS | 15.0% |
| !! | TTATTCTATATAGAATTGAA+TGG | - | chr8.1:19425595-19425614 | MS.gene059920:intron | 15.0% |
| !! | TTTAAACTTGAAATTAACAA+TGG | + | chr8.1:19424697-19424716 | None:intergenic | 15.0% |
| !!! | ATAAAGGATTATTATTTCTA+TGG | + | chr8.1:19425502-19425521 | None:intergenic | 15.0% |
| !! | AACATTCAACAAAAATCATA+AGG | + | chr8.1:19425289-19425308 | None:intergenic | 20.0% |
| !! | ATATAGAATTGAATGGTAAA+AGG | - | chr8.1:19425602-19425621 | MS.gene059920:intron | 20.0% |
| !! | ATATCAACATAAACATTCAT+GGG | + | chr8.1:19425893-19425912 | None:intergenic | 20.0% |
| !! | CAATGCATAAAAAAGAAAAT+GGG | + | chr8.1:19424624-19424643 | None:intergenic | 20.0% |
| !! | TAATACTATAATGATCTTTG+TGG | - | chr8.1:19425747-19425766 | MS.gene059920:intron | 20.0% |
| !! | TCAATGCATAAAAAAGAAAA+TGG | + | chr8.1:19424625-19424644 | None:intergenic | 20.0% |
| !! | TCATGTTAACATAAAAGATT+AGG | - | chr8.1:19425956-19425975 | MS.gene059920:intron | 20.0% |
| !!! | ACACAATTATTGATTTTTCT+AGG | - | chr8.1:19425371-19425390 | MS.gene059920:intron | 20.0% |
| !!! | TCATAGTTATTTTATTGAGA+AGG | + | chr8.1:19425018-19425037 | None:intergenic | 20.0% |
| ! | AAGATAAAAGATGACGATTA+TGG | + | chr8.1:19424517-19424536 | None:intergenic | 25.0% |
| ! | AATCCACAACAATAGTAAAA+AGG | + | chr8.1:19426165-19426184 | None:intergenic | 25.0% |
| ! | CATATCAACATAAACATTCA+TGG | + | chr8.1:19425894-19425913 | None:intergenic | 25.0% |
| ! | GAGGAAAAAAAAAACATCAA+AGG | + | chr8.1:19424893-19424912 | None:intergenic | 25.0% |
| ! | TAAAAAAGAAAATGGGTATC+AGG | + | chr8.1:19424617-19424636 | None:intergenic | 25.0% |
| ! | TATCAACATAAACATTCATG+GGG | + | chr8.1:19425892-19425911 | None:intergenic | 25.0% |
| !! | GATTTTTGTTGAATGTTGTT+TGG | - | chr8.1:19425292-19425311 | MS.gene059920:intron | 25.0% |
| !! | TTTCCTTTTTACTATTGTTG+TGG | - | chr8.1:19426159-19426178 | MS.gene059920:CDS | 25.0% |
| !! | TTTTTATGAGTGAGATTTCT+AGG | - | chr8.1:19424804-19424823 | MS.gene059920:CDS | 25.0% |
| AAGCATGAATCAGAGAAATT+GGG | - | chr8.1:19425037-19425056 | MS.gene059920:intron | 30.0% | |
| AGTAAAGCGTGAAAATTCTT+TGG | - | chr8.1:19425394-19425413 | MS.gene059920:intron | 30.0% | |
| ATTTCTACAACTGTGGTATT+TGG | - | chr8.1:19424727-19424746 | MS.gene059920:intron | 30.0% | |
| TAGCTTATTATAGCAACATG+AGG | + | chr8.1:19425996-19426015 | None:intergenic | 30.0% | |
| TGTTTGGATTGTTTGCAATT+TGG | - | chr8.1:19425308-19425327 | MS.gene059920:intron | 30.0% | |
| TTAGTTGGTTGAATGTTGTT+TGG | - | chr8.1:19425183-19425202 | MS.gene059920:intron | 30.0% | |
| TTATGAGATTTCTACAACTG+TGG | - | chr8.1:19424720-19424739 | MS.gene059920:intron | 30.0% | |
| ! | TATTTGGTAGTCTAAAACTG+TGG | - | chr8.1:19424743-19424762 | MS.gene059920:CDS | 30.0% |
| ! | TCAAGGTAAAGACTTTTAGT+TGG | - | chr8.1:19425168-19425187 | MS.gene059920:intron | 30.0% |
| ! | TTTTCACTTTCATGTACTCT+TGG | - | chr8.1:19424928-19424947 | MS.gene059920:intron | 30.0% |
| !!! | TTTTTTTTCCTCCAGTTGAT+GGG | - | chr8.1:19424901-19424920 | MS.gene059920:intron | 30.0% |
| !!! | TTTTTTTTTCCTCCAGTTGA+TGG | - | chr8.1:19424900-19424919 | MS.gene059920:intron | 30.0% |
| AGAGGTCATACAATCTTACT+TGG | - | chr8.1:19425536-19425555 | MS.gene059920:CDS | 35.0% | |
| CTCACACCCTTAACTAATAA+AGG | + | chr8.1:19425518-19425537 | None:intergenic | 35.0% | |
| CTCTACTTCGTGTATATAAC+GGG | + | chr8.1:19426231-19426250 | None:intergenic | 35.0% | |
| GAAGCATGAATCAGAGAAAT+TGG | - | chr8.1:19425036-19425055 | MS.gene059920:intron | 35.0% | |
| GATTTCTACGATTAACGACA+TGG | - | chr8.1:19424778-19424797 | MS.gene059920:CDS | 35.0% | |
| GCTCTACTTCGTGTATATAA+CGG | + | chr8.1:19426232-19426251 | None:intergenic | 35.0% | |
| GTTTGGATTGTTTGCTCATA+AGG | - | chr8.1:19425200-19425219 | MS.gene059920:intron | 35.0% | |
| TCTTGGATTTGTGTATCTAG+AGG | - | chr8.1:19424945-19424964 | MS.gene059920:intron | 35.0% | |
| TTACCTTGAGAAAGTGAAGT+AGG | + | chr8.1:19425157-19425176 | None:intergenic | 35.0% | |
| TTACTTTCATCTCTCACATG+TGG | - | chr8.1:19425628-19425647 | MS.gene059920:intron | 35.0% | |
| ! | ACTTTTGTGAGTGAAGTTGA+AGG | - | chr8.1:19425842-19425861 | MS.gene059920:intron | 35.0% |
| ! | ATCAGCTACGGCAAAATTTT+CGG | - | chr8.1:19424585-19424604 | MS.gene059920:intron | 35.0% |
| ! | CTTTTGTGAGTGAAGTTGAA+GGG | - | chr8.1:19425843-19425862 | MS.gene059920:intron | 35.0% |
| ! | TTATTAGTTAAGGGTGTGAG+AGG | - | chr8.1:19425518-19425537 | MS.gene059920:CDS | 35.0% |
| AAAAACAACCCATCAACTGG+AGG | + | chr8.1:19424912-19424931 | None:intergenic | 40.0% | |
| AACACTGGAAAGTTGGCTAA+TGG | - | chr8.1:19425809-19425828 | MS.gene059920:intron | 40.0% | |
| AACATGAGCAGTTGCAAGAA+AGG | + | chr8.1:19425924-19425943 | None:intergenic | 40.0% | |
| AAGCACTCACTCACAAATAG+AGG | - | chr8.1:19426085-19426104 | MS.gene059920:CDS | 40.0% | |
| AGTGAAGTAGGAGAGAACAA+CGG | + | chr8.1:19425145-19425164 | None:intergenic | 40.0% | |
| CGTGAATTTGCGAACTGTAA+AGG | - | chr8.1:19424988-19425007 | MS.gene059920:intron | 40.0% | |
| GAGATTGAGACCTTCAAACA+CGG | + | chr8.1:19424426-19424445 | None:intergenic | 40.0% | |
| GTGAAAAACAACCCATCAAC+TGG | + | chr8.1:19424915-19424934 | None:intergenic | 40.0% | |
| GTGGTGTTTGTAACATGTGT+TGG | - | chr8.1:19425766-19425785 | MS.gene059920:intron | 40.0% | |
| TCTCCTACTTCACTTTCTCA+AGG | - | chr8.1:19425151-19425170 | MS.gene059920:intron | 40.0% | |
| TGTTGACCTTGAATCGATTG+TGG | + | chr8.1:19424463-19424482 | None:intergenic | 40.0% | |
| TTGCAACAACTCATCAGCTA+CGG | - | chr8.1:19424573-19424592 | MS.gene059920:intron | 40.0% | |
| !! | GTTGGCTTTACTTCTCTTAC+AGG | - | chr8.1:19425784-19425803 | MS.gene059920:intron | 40.0% |
| !!! | GTACAATTGGCTTTTGCCAT+CGG | - | chr8.1:19425113-19425132 | MS.gene059920:intron | 40.0% |
| AAGCCAATTGTACAGCGTGA+CGG | + | chr8.1:19425106-19425125 | None:intergenic | 45.0% | |
| ACAGGACAACACTGGAAAGT+TGG | - | chr8.1:19425802-19425821 | MS.gene059920:intron | 45.0% | |
| CTTCTCTTACAGGACAACAC+TGG | - | chr8.1:19425794-19425813 | MS.gene059920:intron | 45.0% | |
| GGGTCTGTAATCCATGGAAA+TGG | - | chr8.1:19425863-19425882 | MS.gene059920:intron | 45.0% | |
| GTTGAAGGGTCTGTAATCCA+TGG | - | chr8.1:19425857-19425876 | MS.gene059920:intron | 45.0% | |
| TCTCTCACATGTGGACATCA+TGG | - | chr8.1:19425637-19425656 | MS.gene059920:intron | 45.0% | |
| TGATGAGTTGTTGCAAACCC+TGG | + | chr8.1:19424569-19424588 | None:intergenic | 45.0% | |
| TGTGAGTGAGTGCTTCTCTA+TGG | + | chr8.1:19426080-19426099 | None:intergenic | 45.0% | |
| ! | AGAAATTGGGTTTTGCCGTC+GGG | - | chr8.1:19425050-19425069 | MS.gene059920:intron | 45.0% |
| ! | GAGAAATTGGGTTTTGCCGT+CGG | - | chr8.1:19425049-19425068 | MS.gene059920:intron | 45.0% |
| ! | TCAGAGCCACAATCGATTCA+AGG | - | chr8.1:19424454-19424473 | MS.gene059920:intron | 45.0% |
| !! | TGAATCGATTGTGGCTCTGA+AGG | + | chr8.1:19424454-19424473 | None:intergenic | 45.0% |
| ACCTTCAAACACGGTTGCGA+GGG | + | chr8.1:19424417-19424436 | None:intergenic | 50.0% | |
| AGAACAACGGCAAGATCCGA+TGG | + | chr8.1:19425132-19425151 | None:intergenic | 50.0% | |
| TCACCGTCACGCTGTACAAT+TGG | - | chr8.1:19425100-19425119 | MS.gene059920:intron | 50.0% | |
| TCATGGGGAGACCATTTCCA+TGG | + | chr8.1:19425877-19425896 | None:intergenic | 50.0% | |
| ACCCTCGCAACCGTGTTTGA+AGG | - | chr8.1:19424413-19424432 | MS.gene059920:CDS | 55.0% | |
| ATCCAAGCACGCAAGGTTCC+AGG | - | chr8.1:19424548-19424567 | MS.gene059920:intron | 55.0% | |
| CGAAGTGAACGACAACCCGA+CGG | + | chr8.1:19425068-19425087 | None:intergenic | 55.0% | |
| GACCTTCAAACACGGTTGCG+AGG | + | chr8.1:19424418-19424437 | None:intergenic | 55.0% | |
| TCCAAGCACGCAAGGTTCCA+GGG | - | chr8.1:19424549-19424568 | MS.gene059920:intron | 55.0% | |
| TCTCGCAATCCAAGCACGCA+AGG | - | chr8.1:19424541-19424560 | MS.gene059920:intron | 55.0% | |
| TTGCGTGCTTGGATTGCGAG+AGG | + | chr8.1:19424542-19424561 | None:intergenic | 55.0% | |
| ACCCTGGAACCTTGCGTGCT+TGG | + | chr8.1:19424553-19424572 | None:intergenic | 60.0% | |
| CGGGTTGTCGTTCACTTCGC+AGG | - | chr8.1:19425069-19425088 | MS.gene059920:intron | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr8.1 | gene | 19424398 | 19426276 | 19424398 | ID=MS.gene059920 |
| chr8.1 | mRNA | 19424398 | 19426276 | 19424398 | ID=MS.gene059920.t1;Parent=MS.gene059920 |
| chr8.1 | exon | 19426068 | 19426276 | 19426068 | ID=MS.gene059920.t1.exon1;Parent=MS.gene059920.t1 |
| chr8.1 | CDS | 19426068 | 19426276 | 19426068 | ID=cds.MS.gene059920.t1;Parent=MS.gene059920.t1 |
| chr8.1 | exon | 19425502 | 19425583 | 19425502 | ID=MS.gene059920.t1.exon2;Parent=MS.gene059920.t1 |
| chr8.1 | CDS | 19425502 | 19425583 | 19425502 | ID=cds.MS.gene059920.t1;Parent=MS.gene059920.t1 |
| chr8.1 | exon | 19424728 | 19424868 | 19424728 | ID=MS.gene059920.t1.exon3;Parent=MS.gene059920.t1 |
| chr8.1 | CDS | 19424728 | 19424868 | 19424728 | ID=cds.MS.gene059920.t1;Parent=MS.gene059920.t1 |
| chr8.1 | exon | 19424398 | 19424451 | 19424398 | ID=MS.gene059920.t1.exon4;Parent=MS.gene059920.t1 |
| chr8.1 | CDS | 19424398 | 19424451 | 19424398 | ID=cds.MS.gene059920.t1;Parent=MS.gene059920.t1 |
| Gene Sequence |
| Protein sequence |
