Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57018.t1 | AES98664.2 | 68.1 | 72 | 23 | 0 | 7 | 78 | 962 | 1033 | 3.10E-17 | 97.4 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57018.t1 | G7JZJ5 | 68.1 | 72 | 23 | 0 | 7 | 78 | 962 | 1033 | 2.2e-17 | 97.4 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene054467 | MS.gene57018 | 0.803231 | 3.85E-49 | -1.69E-46 |
| MS.gene054468 | MS.gene57018 | 0.800808 | 1.21E-48 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57018.t1 | MTR_5g071310 | 67.123 | 73 | 24 | 0 | 7 | 79 | 962 | 1034 | 9.30e-27 | 101 |
| MS.gene57018.t1 | MTR_5g070490 | 65.333 | 75 | 22 | 1 | 5 | 79 | 1001 | 1071 | 4.30e-18 | 77.0 |
| MS.gene57018.t1 | MTR_1g007860 | 43.000 | 100 | 30 | 1 | 7 | 79 | 72 | 171 | 9.23e-17 | 73.2 |
| MS.gene57018.t1 | MTR_5g072260 | 72.093 | 43 | 12 | 0 | 26 | 68 | 19 | 61 | 1.56e-15 | 65.5 |
| MS.gene57018.t1 | MTR_5g070470 | 45.098 | 102 | 33 | 6 | 1 | 79 | 997 | 1098 | 1.22e-13 | 64.3 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 2.96e-11 | 57.4 |
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 2.97e-11 | 57.4 |
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 3.19e-11 | 57.4 |
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 3.28e-11 | 57.4 |
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 3.28e-11 | 57.4 |
| MS.gene57018.t1 | AT5G53050 | 40.741 | 81 | 21 | 1 | 25 | 78 | 78 | 158 | 3.32e-11 | 57.4 |
Find 14 sgRNAs with CRISPR-Local
Find 65 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| ATGATGAAGAGAACGTCTTT+TGG | 0.348464 | 5.1:+55048460 | MS.gene57018:intron |
| TTGGATTTGTTATATGCAAC+AGG | 0.375269 | 5.1:+55049073 | MS.gene57018:CDS |
| TCCAAGGAGAGAACTCTATC+AGG | 0.384965 | 5.1:-55049055 | None:intergenic |
| TCCTGATAGAGTTCTCTCCT+TGG | 0.460980 | 5.1:+55049054 | MS.gene57018:CDS |
| TTGATGATCAGACTTGCTAC+TGG | 0.496294 | 5.1:-55048565 | None:intergenic |
| ATCAGACTTGCTACTGGGCA+CGG | 0.543216 | 5.1:-55048559 | None:intergenic |
| CAAGTCCGTTGGCGGTATCG+AGG | 0.549031 | 5.1:+55048500 | MS.gene57018:CDS |
| GGTGACAACAAGTCCGTTGG+CGG | 0.571244 | 5.1:+55048492 | MS.gene57018:CDS |
| TGATGATCAGACTTGCTACT+GGG | 0.599837 | 5.1:-55048564 | None:intergenic |
| AGTAGCAAGTCTGATCATCA+AGG | 0.603717 | 5.1:+55048567 | MS.gene57018:CDS |
| AGAGGTGACAACAAGTCCGT+TGG | 0.614887 | 5.1:+55048489 | MS.gene57018:CDS |
| GATTTGTTATATGCAACAGG+TGG | 0.642658 | 5.1:+55049076 | MS.gene57018:CDS |
| TGTTGCATATAACAAATCCA+AGG | 0.648314 | 5.1:-55049071 | None:intergenic |
| GCAATCCTCGATACCGCCAA+CGG | 0.670975 | 5.1:-55048505 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AAAAATCAAAAAAATCAAAA+AGG | - | chr5.1:55048356-55048375 | None:intergenic | 10.0% |
| !! | CAAAAACTTAAGAATTAAAA+TGG | - | chr5.1:55048599-55048618 | None:intergenic | 15.0% |
| !! | TTGATTTAGTTAAAATTCAT+GGG | + | chr5.1:55048922-55048941 | MS.gene57018:intron | 15.0% |
| !!! | AAGTTTTTGAATGTAATTAT+AGG | + | chr5.1:55048716-55048735 | MS.gene57018:intron | 15.0% |
| !!! | AGTTTTTGAATGTAATTATA+GGG | + | chr5.1:55048717-55048736 | MS.gene57018:intron | 15.0% |
| !!! | TTTAATTCTTAAGTTTTTGT+TGG | + | chr5.1:55048600-55048619 | MS.gene57018:intron | 15.0% |
| !!! | TTTGATTTAGTTAAAATTCA+TGG | + | chr5.1:55048921-55048940 | MS.gene57018:intron | 15.0% |
| !! | ATTATCAAAACTAAGTAAAG+AGG | - | chr5.1:55048681-55048700 | None:intergenic | 20.0% |
| !! | TGATTTAGTTAAAATTCATG+GGG | + | chr5.1:55048923-55048942 | MS.gene57018:intron | 20.0% |
| !! | TTATAATTGCATATTGAAAC+AGG | + | chr5.1:55048998-55049017 | MS.gene57018:intron | 20.0% |
| !!! | TTTTAATTTTGCTGATTTTG+TGG | + | chr5.1:55048372-55048391 | MS.gene57018:intron | 20.0% |
| ! | AAAATATCTTTCATGTACTC+TGG | - | chr5.1:55048257-55048276 | None:intergenic | 25.0% |
| ! | AAGGATAAATGTTCTAATTC+AGG | + | chr5.1:55048292-55048311 | MS.gene57018:intron | 25.0% |
| ! | ATGTCATAGATATGAAATGT+TGG | + | chr5.1:55048761-55048780 | MS.gene57018:intron | 25.0% |
| ! | ATTACTTTGTTGGATCATTT+AGG | + | chr5.1:55048815-55048834 | MS.gene57018:intron | 25.0% |
| ! | TTAGAACATTTATCCTTCTT+AGG | - | chr5.1:55048289-55048308 | None:intergenic | 25.0% |
| ! | TTTGAATGTAATTATAGGGT+TGG | + | chr5.1:55048721-55048740 | MS.gene57018:intron | 25.0% |
| !! | ATCGCAAAAATCAATTTTAC+TGG | + | chr5.1:55048126-55048145 | MS.gene57018:CDS | 25.0% |
| !!! | AGTAAAATTGATTTTTGCGA+TGG | - | chr5.1:55048127-55048146 | None:intergenic | 25.0% |
| !!! | TTTGTGGAAATTGATTTGAA+TGG | + | chr5.1:55048388-55048407 | MS.gene57018:intron | 25.0% |
| AAAGGATGCAATTACTTTGT+TGG | + | chr5.1:55048805-55048824 | MS.gene57018:intron | 30.0% | |
| ACTTAACCTAACCAAATCTT+AGG | - | chr5.1:55048954-55048973 | None:intergenic | 30.0% | |
| TGAAATCCAAGCATAAGAAT+GGG | - | chr5.1:55048166-55048185 | None:intergenic | 30.0% | |
| TGTTGCATATAACAAATCCA+AGG | - | chr5.1:55049074-55049093 | None:intergenic | 30.0% | |
| TTGAAATCCAAGCATAAGAA+TGG | - | chr5.1:55048167-55048186 | None:intergenic | 30.0% | |
| ! | ATCATTAGGTACGTTTGTTA+CGG | - | chr5.1:55048445-55048464 | None:intergenic | 30.0% |
| ! | TTTTACTGTTCAACCTAAGA+AGG | + | chr5.1:55048273-55048292 | MS.gene57018:intron | 30.0% |
| !! | GGAAATTGATTTGAATGGTT+AGG | + | chr5.1:55048393-55048412 | MS.gene57018:intron | 30.0% |
| !! | TTGGATTTGTTATATGCAAC+AGG | + | chr5.1:55049073-55049092 | MS.gene57018:CDS | 30.0% |
| AAGACGTTCTCTTCATCATT+AGG | - | chr5.1:55048459-55048478 | None:intergenic | 35.0% | |
| ACACACAAAATGCTAACCAA+TGG | - | chr5.1:55048870-55048889 | None:intergenic | 35.0% | |
| CTATTTCCCATTCTTATGCT+TGG | + | chr5.1:55048157-55048176 | MS.gene57018:intron | 35.0% | |
| ! | ATGATGAAGAGAACGTCTTT+TGG | + | chr5.1:55048460-55048479 | MS.gene57018:intron | 35.0% |
| ! | GTTTGTCCTAAGATTTGGTT+AGG | + | chr5.1:55048945-55048964 | MS.gene57018:intron | 35.0% |
| !! | AGGATTGCGATTTTGATAGT+TGG | + | chr5.1:55048520-55048539 | MS.gene57018:CDS | 35.0% |
| !! | ATTCTCGTTGTTGACTTCTT+TGG | + | chr5.1:55048209-55048228 | MS.gene57018:intron | 35.0% |
| !! | GATTGCGATTTTGATAGTTG+GGG | + | chr5.1:55048522-55048541 | MS.gene57018:CDS | 35.0% |
| !! | GATTTGTTATATGCAACAGG+TGG | + | chr5.1:55049076-55049095 | MS.gene57018:CDS | 35.0% |
| !! | GGATCATTTAGGTTGCAAAA+AGG | + | chr5.1:55048826-55048845 | MS.gene57018:intron | 35.0% |
| !! | GGATTGCGATTTTGATAGTT+GGG | + | chr5.1:55048521-55048540 | MS.gene57018:CDS | 35.0% |
| AGTAGCAAGTCTGATCATCA+AGG | + | chr5.1:55048567-55048586 | MS.gene57018:CDS | 40.0% | |
| ATGGGGTTTGTCCTAAGATT+TGG | + | chr5.1:55048940-55048959 | MS.gene57018:intron | 40.0% | |
| CAGAACAAAGATCATGGCAA+AGG | + | chr5.1:55048787-55048806 | MS.gene57018:intron | 40.0% | |
| GTTAATCCCTTGTGTGTTGA+AGG | - | chr5.1:55048423-55048442 | None:intergenic | 40.0% | |
| TGGCTACAGAACAAAGATCA+TGG | + | chr5.1:55048781-55048800 | MS.gene57018:intron | 40.0% | |
| ! | CAACAGGTGGATGTTTTCAT+AGG | + | chr5.1:55049089-55049108 | MS.gene57018:CDS | 40.0% |
| !! | CATGTTTTTGTGCACTCCAT+TGG | + | chr5.1:55048851-55048870 | MS.gene57018:intron | 40.0% |
| !! | TGATGATCAGACTTGCTACT+GGG | - | chr5.1:55048567-55048586 | None:intergenic | 40.0% |
| !! | TTGATGATCAGACTTGCTAC+TGG | - | chr5.1:55048568-55048587 | None:intergenic | 40.0% |
| AGGATTGCCTTCAACACACA+AGG | + | chr5.1:55048413-55048432 | MS.gene57018:intron | 45.0% | |
| GGATTGCCTTCAACACACAA+GGG | + | chr5.1:55048414-55048433 | MS.gene57018:intron | 45.0% | |
| TCCAAGGAGAGAACTCTATC+AGG | - | chr5.1:55049058-55049077 | None:intergenic | 45.0% | |
| TCCTGATAGAGTTCTCTCCT+TGG | + | chr5.1:55049054-55049073 | MS.gene57018:CDS | 45.0% | |
| !! | GAAGAGAACGTCTTTTGGAG+TGG | + | chr5.1:55048465-55048484 | MS.gene57018:intron | 45.0% |
| !! | GATTTTGATAGTTGGGGCGT+GGG | + | chr5.1:55048528-55048547 | MS.gene57018:CDS | 45.0% |
| !! | TTCTTTGGAACCCATTACGC+AGG | + | chr5.1:55048224-55048243 | MS.gene57018:intron | 45.0% |
| !!! | AACGTCTTTTGGAGTGGAAG+AGG | + | chr5.1:55048471-55048490 | MS.gene57018:CDS | 45.0% |
| AGAGGTGACAACAAGTCCGT+TGG | + | chr5.1:55048489-55048508 | MS.gene57018:CDS | 50.0% | |
| TGGATCTCTGCCTGCGTAAT+GGG | - | chr5.1:55048237-55048256 | None:intergenic | 50.0% | |
| !! | ATCAGACTTGCTACTGGGCA+CGG | - | chr5.1:55048562-55048581 | None:intergenic | 50.0% |
| !! | CGATTTTGATAGTTGGGGCG+TGG | + | chr5.1:55048527-55048546 | MS.gene57018:CDS | 50.0% |
| CTGGATCTCTGCCTGCGTAA+TGG | - | chr5.1:55048238-55048257 | None:intergenic | 55.0% | |
| GCAATCCTCGATACCGCCAA+CGG | - | chr5.1:55048508-55048527 | None:intergenic | 55.0% | |
| GGTGACAACAAGTCCGTTGG+CGG | + | chr5.1:55048492-55048511 | MS.gene57018:CDS | 55.0% | |
| CAAGTCCGTTGGCGGTATCG+AGG | + | chr5.1:55048500-55048519 | MS.gene57018:CDS | 60.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr5.1 | gene | 55048120 | 55049114 | 55048120 | ID=MS.gene57018 |
| chr5.1 | mRNA | 55048120 | 55049114 | 55048120 | ID=MS.gene57018.t1;Parent=MS.gene57018 |
| chr5.1 | exon | 55048120 | 55048147 | 55048120 | ID=MS.gene57018.t1.exon1;Parent=MS.gene57018.t1 |
| chr5.1 | CDS | 55048120 | 55048147 | 55048120 | ID=cds.MS.gene57018.t1;Parent=MS.gene57018.t1 |
| chr5.1 | exon | 55048469 | 55048588 | 55048469 | ID=MS.gene57018.t1.exon2;Parent=MS.gene57018.t1 |
| chr5.1 | CDS | 55048469 | 55048588 | 55048469 | ID=cds.MS.gene57018.t1;Parent=MS.gene57018.t1 |
| chr5.1 | exon | 55049020 | 55049114 | 55049020 | ID=MS.gene57018.t1.exon3;Parent=MS.gene57018.t1 |
| chr5.1 | CDS | 55049020 | 55049114 | 55049020 | ID=cds.MS.gene57018.t1;Parent=MS.gene57018.t1 |
| Gene Sequence |
| Protein sequence |
