AlfalfaGEDB Alfalfa Gene Editing Database

M. sativa cultivar XinJiangDaYe / MS.gene57256

Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57256.t1 MTR_1g016110 99.675 308 1 0 1 308 1 308 0.0 625
MS.gene57256.t1 MTR_1g016110 99.675 308 1 0 1 308 48 355 0.0 622
MS.gene57256.t1 MTR_1g019620 99.026 308 3 0 1 308 48 355 0.0 618
MS.gene57256.t1 MTR_1g070360 34.564 298 143 10 7 296 17 270 1.17e-42 148
MS.gene57256.t1 MTR_6g086600 40.000 180 103 3 8 187 164 338 3.36e-31 122
MS.gene57256.t1 MTR_6g086600 40.000 180 103 3 8 187 173 347 3.54e-31 122
Query id Subject id identity % alignment length mismatches gap openings q. start q. end s. start s. end e-value bit score
MS.gene57256.t1 AT5G50960 76.393 305 69 2 1 304 48 350 3.09e-171 478
MS.gene57256.t1 AT4G19540 43.005 193 100 5 7 196 38 223 2.56e-38 138
MS.gene57256.t1 AT3G24430 39.444 180 104 3 8 187 172 346 3.69e-31 122

Find 77 sgRNAs with CRISPR-Local

Find 89 sgRNAs with CRISPR-GE


CRISPR-Local

CRISPR-Local
sgRNA_sequence on_target_score Position Region
GCGATTAAATTCAGCATTTC+AGG 0.187756 1.4:+6247687 None:intergenic
CCTTCTAGGCCAAGCATCTT+TGG 0.224706 1.4:+6248188 None:intergenic
ATCTTTCATCTCACCATTAT+CGG 0.267237 1.4:+6247749 None:intergenic
GGTGTATCTCTTGACCTTCT+AGG 0.281189 1.4:+6248174 None:intergenic
GTCTATGTTGAGTCTAATCT+TGG 0.283572 1.4:-6248128 MS.gene57256:CDS
AATCTTGGGGTCATGTCAAT+TGG 0.295181 1.4:-6248113 MS.gene57256:CDS
ATCCAGACGAAGCTGTTATA+TGG 0.325193 1.4:-6248076 MS.gene57256:CDS
TTTATGAAGATTACCGATAA+TGG 0.328286 1.4:-6247762 MS.gene57256:CDS
GAGATGGCGGTACCCTTTCT+TGG 0.328779 1.4:-6247612 MS.gene57256:CDS
CTTCTAGGCCAAGCATCTTT+GGG 0.359162 1.4:+6248189 None:intergenic
AAAGTTGGAGTGAAAGTTCT+TGG 0.364693 1.4:-6247834 MS.gene57256:CDS
TTTCACAGTGGCCATTCTTT+CGG 0.375809 1.4:+6248343 None:intergenic
CTAATTGTCGATGCTCCGCC+TGG 0.379301 1.4:-6247984 MS.gene57256:CDS
CAGCTAACGCAAATGCCAGC+TGG 0.379548 1.4:+6248264 None:intergenic
CCAAAGATGCTTGGCCTAGA+AGG 0.402010 1.4:-6248188 MS.gene57256:CDS
CCAAGCATCTTTGGGATGCT+TGG 0.406720 1.4:+6248197 None:intergenic
TCTATGTTGAGTCTAATCTT+GGG 0.416626 1.4:-6248127 MS.gene57256:CDS
CACTGTGAAACATAAGATAC+TGG 0.420840 1.4:-6248330 MS.gene57256:CDS
AAGTTGGAGTGAAAGTTCTT+GGG 0.429446 1.4:-6247833 MS.gene57256:CDS
GGCGGTACCCTTTCTTGGTA+AGG 0.430728 1.4:-6247607 MS.gene57256:CDS
ATCTTGGGGTCATGTCAATT+GGG 0.431936 1.4:-6248112 MS.gene57256:CDS
CATAAGATACTGGTCTTGTC+AGG 0.432544 1.4:-6248320 MS.gene57256:CDS
TAAAGGAACCTTACCAAGAA+AGG 0.434925 1.4:+6247599 None:intergenic
AGTGAAAGTTCTTGGGGTTG+TGG 0.435467 1.4:-6247826 MS.gene57256:CDS
AGATGTTACGGAGTGGATAT+CGG 0.437420 1.4:-6247730 MS.gene57256:CDS
ACTTGAGATTTGAGATGGGC+TGG 0.443213 1.4:+6247784 None:intergenic
AGCTAACGCAAATGCCAGCT+GGG 0.447107 1.4:+6248265 None:intergenic
CTCCATATAACAGCTTCGTC+TGG 0.454307 1.4:+6248074 None:intergenic
CATAAACTTGAGATTTGAGA+TGG 0.455458 1.4:+6247779 None:intergenic
CGTCTACATTAGCAGCATCT+AGG 0.458881 1.4:+6247928 None:intergenic
ATACTGGTCTTGTCAGGAAA+AGG 0.475392 1.4:-6248314 MS.gene57256:CDS
CTTTGCAAGGCAGCTGAAGA+AGG 0.475604 1.4:-6247567 MS.gene57256:CDS
TGGTGAGATGAAAGATGTTA+CGG 0.483466 1.4:-6247742 MS.gene57256:CDS
ATGTGTTCATCTGAGGTTCC+AGG 0.485714 1.4:+6247966 None:intergenic
GGCATTTGCGTTAGCTGCAA+GGG 0.486589 1.4:-6248258 MS.gene57256:CDS
GAGACCAACCAAGGTTGCTC+TGG 0.487893 1.4:+6248153 None:intergenic
CTTCTGGACATTGATATTTG+CGG 0.495696 1.4:-6248221 MS.gene57256:CDS
GATTTGAGATGGGCTGGGAC+AGG 0.504393 1.4:+6247790 None:intergenic
TTGCAAAGCTGTGGATCTAA+AGG 0.505094 1.4:+6247582 None:intergenic
GGTTGCCATTGCCGAAAGAA+TGG 0.507040 1.4:-6248354 MS.gene57256:CDS
CTTGAGATTTGAGATGGGCT+GGG 0.511450 1.4:+6247785 None:intergenic
GTATTTGATAGTAGCGGTGG+TGG 0.514266 1.4:-6247654 MS.gene57256:CDS
TCAGCTGCCTTGCAAAGCTG+TGG 0.521436 1.4:+6247573 None:intergenic
AAAGGAACCTTACCAAGAAA+GGG 0.525559 1.4:+6247600 None:intergenic
TGGCATTTGCGTTAGCTGCA+AGG 0.531152 1.4:-6248259 MS.gene57256:CDS
GATAAGCTGATGGAAACTAG+TGG 0.534659 1.4:-6247483 MS.gene57256:CDS
AGATTAGACTCAACATAGAC+AGG 0.537946 1.4:+6248131 None:intergenic
CCAAGCATCCCAAAGATGCT+TGG 0.539567 1.4:-6248197 MS.gene57256:CDS
GAGATACACCAGAGCAACCT+TGG 0.541471 1.4:-6248161 MS.gene57256:CDS
AGTGGCCATTCTTTCGGCAA+TGG 0.546079 1.4:+6248349 None:intergenic
TACACCAGAGCAACCTTGGT+TGG 0.551299 1.4:-6248157 MS.gene57256:CDS
AGTTGGAGTGAAAGTTCTTG+GGG 0.553652 1.4:-6247832 MS.gene57256:CDS
ATAACAGCTTCGTCTGGATG+AGG 0.554608 1.4:+6248080 None:intergenic
AATTGAAATGTGTTCATCTG+AGG 0.559191 1.4:+6247959 None:intergenic
ATAAACTTGAGATTTGAGAT+GGG 0.559421 1.4:+6247780 None:intergenic
TTGTCAGGAAAAGGAGGTGT+TGG 0.559918 1.4:-6248305 MS.gene57256:CDS
AAAGATAATAGATAAGCTGA+TGG 0.562227 1.4:-6247493 MS.gene57256:CDS
AACTAGTGGGCTGTCAATGA+CGG 0.568788 1.4:-6247469 MS.gene57256:CDS
ACTATCAAATACTTCACTGC+AGG 0.571986 1.4:+6247665 None:intergenic
CTATGTTGAGTCTAATCTTG+GGG 0.574536 1.4:-6248126 MS.gene57256:CDS
CTGTCAATGACGGAAAGCAA+TGG 0.575375 1.4:-6247459 MS.gene57256:CDS
GACGAAGCTGTTATATGGAG+AGG 0.584494 1.4:-6248071 MS.gene57256:CDS
ATAAGCTGATGGAAACTAGT+GGG 0.602164 1.4:-6247482 MS.gene57256:CDS
TTTAGATCCACAGCTTTGCA+AGG 0.603851 1.4:-6247580 MS.gene57256:CDS
GTTGAAGATGTGTAACGAGA+TGG 0.614173 1.4:-6247628 MS.gene57256:CDS
CTGGTCTTGTCAGGAAAAGG+AGG 0.619582 1.4:-6248311 MS.gene57256:CDS
AAGAGAGACTTGTTGAGGAG+TGG 0.628280 1.4:+6247890 None:intergenic
AGATGAAAGATGTTACGGAG+TGG 0.628653 1.4:-6247737 MS.gene57256:CDS
CTAGATGCTGCTAATGTAGA+CGG 0.629983 1.4:-6247927 MS.gene57256:CDS
GAAGTATTTGATAGTAGCGG+TGG 0.649921 1.4:-6247657 MS.gene57256:CDS
TCAATAAGAGAGACTTGTTG+AGG 0.650660 1.4:+6247885 None:intergenic
GGGGTTGTGGAGAATATGAG+TGG 0.671351 1.4:-6247813 MS.gene57256:CDS
AGTGAAGTATTTGATAGTAG+CGG 0.680092 1.4:-6247660 MS.gene57256:CDS
CATAGACAGGAGACCAACCA+AGG 0.702138 1.4:+6248144 None:intergenic
GAAGATGTGTAACGAGATGG+CGG 0.710160 1.4:-6247625 MS.gene57256:CDS
CAGTATCTTATGTTTCACAG+TGG 0.751734 1.4:+6248331 None:intergenic
TGTTCATCTGAGGTTCCAGG+CGG 0.754863 1.4:+6247969 None:intergenic

CRISPR-GE

badsite warning sgRNA_sequence Strand Position Region GC_content
!!! AGTTTTTAAAAGACGTTTAT+TGG - chr1.4:6247786-6247805 MS.gene57256:CDS 20.0%
!!! GTTTTTAAAAGACGTTTATT+GGG - chr1.4:6247787-6247806 MS.gene57256:CDS 20.0%
!!! TTTTTAAAAGACGTTTATTG+GGG - chr1.4:6247788-6247807 MS.gene57256:CDS 20.0%
! AAAGATAATAGATAAGCTGA+TGG - chr1.4:6248315-6248334 MS.gene57256:CDS 25.0%
! TTTATGAAGATTACCGATAA+TGG - chr1.4:6248046-6248065 MS.gene57256:CDS 25.0%
!! ATAAACTTGAGATTTGAGAT+GGG + chr1.4:6248031-6248050 None:intergenic 25.0%
!! TCTATTATCTTTTGTAATGC+TGG + chr1.4:6248307-6248326 None:intergenic 25.0%
AATTGAAATGTGTTCATCTG+AGG + chr1.4:6247852-6247871 None:intergenic 30.0%
ATCTTTCATCTCACCATTAT+CGG + chr1.4:6248062-6248081 None:intergenic 30.0%
CATAAACTTGAGATTTGAGA+TGG + chr1.4:6248032-6248051 None:intergenic 30.0%
TCTATGTTGAGTCTAATCTT+GGG - chr1.4:6247681-6247700 MS.gene57256:CDS 30.0%
! AGTGAAGTATTTGATAGTAG+CGG - chr1.4:6248148-6248167 MS.gene57256:CDS 30.0%
! GTGAATTTTTGCAAGAAAGT+TGG - chr1.4:6247959-6247978 MS.gene57256:CDS 30.0%
AAAGGAACCTTACCAAGAAA+GGG + chr1.4:6248211-6248230 None:intergenic 35.0%
AAAGTTGGAGTGAAAGTTCT+TGG - chr1.4:6247974-6247993 MS.gene57256:CDS 35.0%
AAGTTGGAGTGAAAGTTCTT+GGG - chr1.4:6247975-6247994 MS.gene57256:CDS 35.0%
ACTATCAAATACTTCACTGC+AGG + chr1.4:6248146-6248165 None:intergenic 35.0%
AGATTAGACTCAACATAGAC+AGG + chr1.4:6247680-6247699 None:intergenic 35.0%
ATAAGCTGATGGAAACTAGT+GGG - chr1.4:6248326-6248345 MS.gene57256:CDS 35.0%
CACTGTGAAACATAAGATAC+TGG - chr1.4:6247478-6247497 MS.gene57256:CDS 35.0%
CAGTATCTTATGTTTCACAG+TGG + chr1.4:6247480-6247499 None:intergenic 35.0%
CTATGTTGAGTCTAATCTTG+GGG - chr1.4:6247682-6247701 MS.gene57256:CDS 35.0%
CTTCTGGACATTGATATTTG+CGG - chr1.4:6247587-6247606 MS.gene57256:CDS 35.0%
GCGATTAAATTCAGCATTTC+AGG + chr1.4:6248124-6248143 None:intergenic 35.0%
GTCTATGTTGAGTCTAATCT+TGG - chr1.4:6247680-6247699 MS.gene57256:CDS 35.0%
TAAAGGAACCTTACCAAGAA+AGG + chr1.4:6248212-6248231 None:intergenic 35.0%
TGGTGAGATGAAAGATGTTA+CGG - chr1.4:6248066-6248085 MS.gene57256:CDS 35.0%
!! TCAATAAGAGAGACTTGTTG+AGG + chr1.4:6247926-6247945 None:intergenic 35.0%
!! TTGATAAGACCATTTTTGCG+TGG + chr1.4:6247764-6247783 None:intergenic 35.0%
AATCTTGGGGTCATGTCAAT+TGG - chr1.4:6247695-6247714 MS.gene57256:CDS 40.0%
AGATGAAAGATGTTACGGAG+TGG - chr1.4:6248071-6248090 MS.gene57256:CDS 40.0%
AGATGTTACGGAGTGGATAT+CGG - chr1.4:6248078-6248097 MS.gene57256:CDS 40.0%
AGTTGGAGTGAAAGTTCTTG+GGG - chr1.4:6247976-6247995 MS.gene57256:CDS 40.0%
ATCCAGACGAAGCTGTTATA+TGG - chr1.4:6247732-6247751 MS.gene57256:CDS 40.0%
ATCTTGGGGTCATGTCAATT+GGG - chr1.4:6247696-6247715 MS.gene57256:CDS 40.0%
CTAGATGCTGCTAATGTAGA+CGG - chr1.4:6247881-6247900 MS.gene57256:CDS 40.0%
GATAAGCTGATGGAAACTAG+TGG - chr1.4:6248325-6248344 MS.gene57256:CDS 40.0%
GTTGAAGATGTGTAACGAGA+TGG - chr1.4:6248180-6248199 MS.gene57256:CDS 40.0%
TTGCAAAGCTGTGGATCTAA+AGG + chr1.4:6248229-6248248 None:intergenic 40.0%
TTTAGATCCACAGCTTTGCA+AGG - chr1.4:6248228-6248247 MS.gene57256:CDS 40.0%
TTTCACAGTGGCCATTCTTT+CGG + chr1.4:6247468-6247487 None:intergenic 40.0%
! GAAGTATTTGATAGTAGCGG+TGG - chr1.4:6248151-6248170 MS.gene57256:CDS 40.0%
! TAGATCGTGTTTTGCAGGTA+AGG - chr1.4:6248264-6248283 MS.gene57256:CDS 40.0%
!! ATACTGGTCTTGTCAGGAAA+AGG - chr1.4:6247494-6247513 MS.gene57256:CDS 40.0%
!! CATAAGATACTGGTCTTGTC+AGG - chr1.4:6247488-6247507 MS.gene57256:CDS 40.0%
!!! GGATTTTCAAGTTGGTCTTC+TGG - chr1.4:6247571-6247590 MS.gene57256:CDS 40.0%
AGTGAAAGTTCTTGGGGTTG+TGG - chr1.4:6247982-6248001 MS.gene57256:CDS 45.0%
ATAACAGCTTCGTCTGGATG+AGG + chr1.4:6247731-6247750 None:intergenic 45.0%
CGTCTACATTAGCAGCATCT+AGG + chr1.4:6247883-6247902 None:intergenic 45.0%
CTCCATATAACAGCTTCGTC+TGG + chr1.4:6247737-6247756 None:intergenic 45.0%
CTTCTAGGCCAAGCATCTTT+GGG + chr1.4:6247622-6247641 None:intergenic 45.0%
GAAGATGTGTAACGAGATGG+CGG - chr1.4:6248183-6248202 MS.gene57256:CDS 45.0%
GACGAAGCTGTTATATGGAG+AGG - chr1.4:6247737-6247756 MS.gene57256:CDS 45.0%
GGTGTATCTCTTGACCTTCT+AGG + chr1.4:6247637-6247656 None:intergenic 45.0%
! ACTTGAGATTTGAGATGGGC+TGG + chr1.4:6248027-6248046 None:intergenic 45.0%
! ATGTGTTCATCTGAGGTTCC+AGG + chr1.4:6247845-6247864 None:intergenic 45.0%
! CTGTCAATGACGGAAAGCAA+TGG - chr1.4:6248349-6248368 MS.gene57256:CDS 45.0%
! CTTGAGATTTGAGATGGGCT+GGG + chr1.4:6248026-6248045 None:intergenic 45.0%
! GAAGGTAGATCGTGTTTTGC+AGG - chr1.4:6248259-6248278 MS.gene57256:CDS 45.0%
! GTATTTGATAGTAGCGGTGG+TGG - chr1.4:6248154-6248173 MS.gene57256:CDS 45.0%
! TTGTCAGGAAAAGGAGGTGT+TGG - chr1.4:6247503-6247522 MS.gene57256:CDS 45.0%
!! AACTAGTGGGCTGTCAATGA+CGG - chr1.4:6248339-6248358 MS.gene57256:CDS 45.0%
!! AAGAGAGACTTGTTGAGGAG+TGG + chr1.4:6247921-6247940 None:intergenic 45.0%
!!! GCTGCAAGGGATTTTCAAGT+TGG - chr1.4:6247563-6247582 MS.gene57256:CDS 45.0%
AGCTAACGCAAATGCCAGCT+GGG + chr1.4:6247546-6247565 None:intergenic 50.0%
AGTGGCCATTCTTTCGGCAA+TGG + chr1.4:6247462-6247481 None:intergenic 50.0%
CATAGACAGGAGACCAACCA+AGG + chr1.4:6247667-6247686 None:intergenic 50.0%
CCAAAGATGCTTGGCCTAGA+AGG - chr1.4:6247620-6247639 MS.gene57256:CDS 50.0%
CCAAGCATCCCAAAGATGCT+TGG - chr1.4:6247611-6247630 MS.gene57256:CDS 50.0%
CCTTCTAGGCCAAGCATCTT+TGG + chr1.4:6247623-6247642 None:intergenic 50.0%
CTTTGCAAGGCAGCTGAAGA+AGG - chr1.4:6248241-6248260 MS.gene57256:CDS 50.0%
GAGATACACCAGAGCAACCT+TGG - chr1.4:6247647-6247666 MS.gene57256:CDS 50.0%
GGCATTTGCGTTAGCTGCAA+GGG - chr1.4:6247550-6247569 MS.gene57256:CDS 50.0%
GGGGTTGTGGAGAATATGAG+TGG - chr1.4:6247995-6248014 MS.gene57256:CDS 50.0%
GGTTGCCATTGCCGAAAGAA+TGG - chr1.4:6247454-6247473 MS.gene57256:CDS 50.0%
TACACCAGAGCAACCTTGGT+TGG - chr1.4:6247651-6247670 MS.gene57256:CDS 50.0%
TGGAGAGGTCCACGCAAAAA+TGG - chr1.4:6247752-6247771 MS.gene57256:CDS 50.0%
TGGCATTTGCGTTAGCTGCA+AGG - chr1.4:6247549-6247568 MS.gene57256:CDS 50.0%
! TGTTCATCTGAGGTTCCAGG+CGG + chr1.4:6247842-6247861 None:intergenic 50.0%
!! CCAAGCATCTTTGGGATGCT+TGG + chr1.4:6247614-6247633 None:intergenic 50.0%
!! CTGGTCTTGTCAGGAAAAGG+AGG - chr1.4:6247497-6247516 MS.gene57256:CDS 50.0%
CAGCTAACGCAAATGCCAGC+TGG + chr1.4:6247547-6247566 None:intergenic 55.0%
CTAATTGTCGATGCTCCGCC+TGG - chr1.4:6247824-6247843 MS.gene57256:CDS 55.0%
GAGACCAACCAAGGTTGCTC+TGG + chr1.4:6247658-6247677 None:intergenic 55.0%
GGCGGTACCCTTTCTTGGTA+AGG - chr1.4:6248201-6248220 MS.gene57256:CDS 55.0%
TCAGCTGCCTTGCAAAGCTG+TGG + chr1.4:6248238-6248257 None:intergenic 55.0%
! GAGATGGCGGTACCCTTTCT+TGG - chr1.4:6248196-6248215 MS.gene57256:CDS 55.0%
! GAGCACATTTTCTGCCCAGC+TGG - chr1.4:6247529-6247548 MS.gene57256:CDS 55.0%
!! GATTTGAGATGGGCTGGGAC+AGG + chr1.4:6248021-6248040 None:intergenic 55.0%


Chromosome Type Strat End Strand Name
chr1.4 gene 6247452 6248378 6247452 ID=MS.gene57256
chr1.4 mRNA 6247452 6248378 6247452 ID=MS.gene57256.t1;Parent=MS.gene57256
chr1.4 exon 6247452 6248378 6247452 ID=MS.gene57256.t1.exon1;Parent=MS.gene57256.t1
chr1.4 CDS 6247452 6248378 6247452 ID=cds.MS.gene57256.t1;Parent=MS.gene57256.t1
Gene Sequence

>MS.gene57256

ATGGTTGCCATTGCCGAAAGAATGGCCACTGTGAAACATAAGATACTGGTCTTGTCAGGAAAAGGAGGTGTTGGCAAGAGCACATTTTCTGCCCAGCTGGCATTTGCGTTAGCTGCAAGGGATTTTCAAGTTGGTCTTCTGGACATTGATATTTGCGGTCCAAGCATCCCAAAGATGCTTGGCCTAGAAGGTCAAGAGATACACCAGAGCAACCTTGGTTGGTCTCCTGTCTATGTTGAGTCTAATCTTGGGGTCATGTCAATTGGGTTCATGCTTCCTCATCCAGACGAAGCTGTTATATGGAGAGGTCCACGCAAAAATGGTCTTATCAAGCAGTTTTTAAAAGACGTTTATTGGGGTGAACTTGATTTTCTAATTGTCGATGCTCCGCCTGGAACCTCAGATGAACACATTTCAATTGTTCAATGCCTAGATGCTGCTAATGTAGACGGTGCAATCATAGTTACCACTCCTCAACAAGTCTCTCTTATTGATGTGAAAAAAGAAGTGAATTTTTGCAAGAAAGTTGGAGTGAAAGTTCTTGGGGTTGTGGAGAATATGAGTGGCCTGTCCCAGCCCATCTCAAATCTCAAGTTTATGAAGATTACCGATAATGGTGAGATGAAAGATGTTACGGAGTGGATATCGGAGTACATGAAAGAAAAAGCGCCTGAAATGCTGAATTTAATCGCCTGCAGTGAAGTATTTGATAGTAGCGGTGGTGGAGCGTTGAAGATGTGTAACGAGATGGCGGTACCCTTTCTTGGTAAGGTTCCTTTAGATCCACAGCTTTGCAAGGCAGCTGAAGAAGGTAGATCGTGTTTTGCAGGTAAGGATTGTGTCGTGAGCGCTCCAGCATTACAAAAGATAATAGATAAGCTGATGGAAACTAGTGGGCTGTCAATGACGGAAAGCAATGGAGTGTAG
Protein sequence

>MS.gene57256.t1

MVAIAERMATVKHKILVLSGKGGVGKSTFSAQLAFALAARDFQVGLLDIDICGPSIPKMLGLEGQEIHQSNLGWSPVYVESNLGVMSIGFMLPHPDEAVIWRGPRKNGLIKQFLKDVYWGELDFLIVDAPPGTSDEHISIVQCLDAANVDGAIIVTTPQQVSLIDVKKEVNFCKKVGVKVLGVVENMSGLSQPISNLKFMKITDNGEMKDVTEWISEYMKEKAPEMLNLIACSEVFDSSGGGALKMCNEMAVPFLGKVPLDPQLCKAAEEGRSCFAGKDCVVSAPALQKIIDKLMETSGLSMTESNGV