Alfalfa Gene Editing Database
Alfalfa Gene Editing Database
Nr:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57332.t1 | KEH20784.1 | 93 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 8.80E-53 | 216.1 |
Swissprot:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Trembl:
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57332.t1 | A0A072TTE4 | 93.0 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 6.3e-53 | 216.1 |
TFs/TRs:
| Gene ID | Type | Classification |
|---|
Protein Kinases:
| Gene ID | Type | Classification |
|---|
Network:
Co-expression Network:
| Gene1 | Gene2 | correlation coefficient | p_value | FDR |
|---|---|---|---|---|
| MS.gene052091 | MS.gene57332 | 0.821356 | 4.22E-53 | -1.69E-46 |
| MS.gene053932 | MS.gene57332 | 0.815863 | 7.43E-52 | -1.69E-46 |
PPI:
| Gene1 | Gene2 | Type |
|---|
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|---|---|---|---|---|---|---|---|---|---|---|
| MS.gene57332.t1 | MTR_8g089920 | 93.043 | 115 | 8 | 0 | 1 | 115 | 1 | 115 | 7.97e-78 | 226 |
| MS.gene57332.t1 | MTR_3g086830 | 42.017 | 119 | 59 | 3 | 1 | 109 | 4 | 122 | 5.18e-16 | 69.7 |
| Query id | Subject id | identity % | alignment length | mismatches | gap openings | q. start | q. end | s. start | s. end | e-value | bit score |
|---|
Find 20 sgRNAs with CRISPR-Local
Find 37 sgRNAs with CRISPR-GE
CRISPR-Local
| sgRNA_sequence | on_target_score | Position | Region |
|---|---|---|---|
| AATATGGTTAATTTCAATTA+TGG | 0.201378 | 4.1:-10644102 | MS.gene57332:CDS |
| TGATTTCCCTATCTCTGATT+TGG | 0.302555 | 4.1:+10644040 | None:intergenic |
| CAAGAAGCCAAATCAGAGAT+AGG | 0.355674 | 4.1:-10644047 | MS.gene57332:CDS |
| GAGAAGGTTTGAGGAGATTG+AGG | 0.374689 | 4.1:+10644210 | None:intergenic |
| AATCTGTCACACTAGAAGTT+TGG | 0.436538 | 4.1:+10644136 | None:intergenic |
| GATTTGGCTTCTTGAATCAA+AGG | 0.476574 | 4.1:+10644056 | None:intergenic |
| AATTGCTGAGGTTGAAGAGA+AGG | 0.495615 | 4.1:+10644194 | None:intergenic |
| GAGGTTGAAGAGAAGGTTTG+AGG | 0.504838 | 4.1:+10644201 | None:intergenic |
| TAGAAGTTTGGCGGCGGTGA+CGG | 0.511622 | 4.1:+10644148 | None:intergenic |
| TTGTCAGAGTTAGAAGCAGC+AGG | 0.525527 | 4.1:-10644339 | MS.gene57332:CDS |
| AAGAAGCCAAATCAGAGATA+GGG | 0.536255 | 4.1:-10644046 | MS.gene57332:CDS |
| ATCAGAGATAGGGAAATCAA+TGG | 0.542074 | 4.1:-10644036 | MS.gene57332:CDS |
| AAGTTTGGCGGCGGTGACGG+CGG | 0.545140 | 4.1:+10644151 | None:intergenic |
| CTGTCACACTAGAAGTTTGG+CGG | 0.564777 | 4.1:+10644139 | None:intergenic |
| TCAGCAATTTATATGTCTCA+TGG | 0.564801 | 4.1:-10644180 | MS.gene57332:CDS |
| GGAAATCAATGGAGATAACC+AGG | 0.585965 | 4.1:-10644025 | MS.gene57332:intron |
| TCAAATGACAAACAACTACC+TGG | 0.600195 | 4.1:-10644297 | MS.gene57332:CDS |
| TCACACTAGAAGTTTGGCGG+CGG | 0.649605 | 4.1:+10644142 | None:intergenic |
| ATGAGACATATAAATTGCTG+AGG | 0.658796 | 4.1:+10644182 | None:intergenic |
| AAGCAATAACAACAAACACC+AGG | 0.676498 | 4.1:+10644279 | None:intergenic |
CRISPR-GE
| badsite warning | sgRNA_sequence | Strand | Position | Region | GC_content |
|---|---|---|---|---|---|
| !! | AATATGGTTAATTTCAATTA+TGG | - | chr4.1:10643923-10643942 | MS.gene57332:intron | 15.0% |
| !! | CAATGATGATAATTATTATA+AGG | + | chr4.1:10644292-10644311 | None:intergenic | 15.0% |
| !!! | AGATTCTTTTTCTTCAAATA+TGG | - | chr4.1:10643907-10643926 | MS.gene57332:intron | 20.0% |
| !! | ATCATAAGAAAAGAAGCTTT+TGG | - | chr4.1:10644026-10644045 | MS.gene57332:CDS | 25.0% |
| !! | TTCTTTTCTTATGATTTACC+TGG | + | chr4.1:10644021-10644040 | None:intergenic | 25.0% |
| ATGAGACATATAAATTGCTG+AGG | + | chr4.1:10643846-10643865 | None:intergenic | 30.0% | |
| TCAGCAATTTATATGTCTCA+TGG | - | chr4.1:10643845-10643864 | MS.gene57332:intron | 30.0% | |
| AAGAAGCCAAATCAGAGATA+GGG | - | chr4.1:10643979-10643998 | MS.gene57332:intron | 35.0% | |
| AAGCAATAACAACAAACACC+AGG | + | chr4.1:10643749-10643768 | None:intergenic | 35.0% | |
| AATCTGTCACACTAGAAGTT+TGG | + | chr4.1:10643892-10643911 | None:intergenic | 35.0% | |
| ATCAGAGATAGGGAAATCAA+TGG | - | chr4.1:10643989-10644008 | MS.gene57332:intron | 35.0% | |
| GCTACTCAAATTGACAAGAA+AGG | - | chr4.1:10644054-10644073 | MS.gene57332:CDS | 35.0% | |
| TCAAATGACAAACAACTACC+TGG | - | chr4.1:10643728-10643747 | MS.gene57332:intron | 35.0% | |
| TGATTTCCCTATCTCTGATT+TGG | + | chr4.1:10643988-10644007 | None:intergenic | 35.0% | |
| ! | GATTTGGCTTCTTGAATCAA+AGG | + | chr4.1:10643972-10643991 | None:intergenic | 35.0% |
| AAGAAGCTTTCACACGTTCA+AGG | - | chr4.1:10644083-10644102 | MS.gene57332:CDS | 40.0% | |
| AATTGCTGAGGTTGAAGAGA+AGG | + | chr4.1:10643834-10643853 | None:intergenic | 40.0% | |
| CAAGAAGCCAAATCAGAGAT+AGG | - | chr4.1:10643978-10643997 | MS.gene57332:intron | 40.0% | |
| GGAAATCAATGGAGATAACC+AGG | - | chr4.1:10644000-10644019 | MS.gene57332:intron | 40.0% | |
| TGAACGTGTGAAAGCTTCTT+TGG | + | chr4.1:10644083-10644102 | None:intergenic | 40.0% | |
| AAACAATGATGCAACGCCTC+AGG | + | chr4.1:10644333-10644352 | None:intergenic | 45.0% | |
| CTGTCACACTAGAAGTTTGG+CGG | + | chr4.1:10643889-10643908 | None:intergenic | 45.0% | |
| GAGAAGGTTTGAGGAGATTG+AGG | + | chr4.1:10643818-10643837 | None:intergenic | 45.0% | |
| GAGGTTGAAGAGAAGGTTTG+AGG | + | chr4.1:10643827-10643846 | None:intergenic | 45.0% | |
| TTGTCAGAGTTAGAAGCAGC+AGG | - | chr4.1:10643686-10643705 | MS.gene57332:intron | 45.0% | |
| ! | AGGCGTTGCATCATTGTTTC+AGG | - | chr4.1:10644334-10644353 | MS.gene57332:CDS | 45.0% |
| ! | TAGTTTTCAACCGTTGCCTG+AGG | - | chr4.1:10644314-10644333 | MS.gene57332:CDS | 45.0% |
| ACACGTTCAAGGACAGTGAG+GGG | - | chr4.1:10644094-10644113 | MS.gene57332:CDS | 50.0% | |
| CACACGTTCAAGGACAGTGA+GGG | - | chr4.1:10644093-10644112 | MS.gene57332:CDS | 50.0% | |
| TCACACGTTCAAGGACAGTG+AGG | - | chr4.1:10644092-10644111 | MS.gene57332:CDS | 50.0% | |
| TCACACTAGAAGTTTGGCGG+CGG | + | chr4.1:10643886-10643905 | None:intergenic | 50.0% | |
| AAGGACAGTGAGGGGGAGAA+TGG | - | chr4.1:10644102-10644121 | MS.gene57332:CDS | 55.0% | |
| AGGACAGTGAGGGGGAGAAT+GGG | - | chr4.1:10644103-10644122 | MS.gene57332:CDS | 55.0% | |
| CACGTTCAAGGACAGTGAGG+GGG | - | chr4.1:10644095-10644114 | MS.gene57332:CDS | 55.0% | |
| TAGAAGTTTGGCGGCGGTGA+CGG | + | chr4.1:10643880-10643899 | None:intergenic | 55.0% | |
| TGATGCAACGCCTCAGGCAA+CGG | + | chr4.1:10644327-10644346 | None:intergenic | 55.0% | |
| ! | AAGTTTGGCGGCGGTGACGG+CGG | + | chr4.1:10643877-10643896 | None:intergenic | 65.0% |
| Chromosome | Type | Strat | End | Strand | Name |
|---|---|---|---|---|---|
| chr4.1 | gene | 10643680 | 10644367 | 10643680 | ID=MS.gene57332 |
| chr4.1 | mRNA | 10643680 | 10644367 | 10643680 | ID=MS.gene57332.t1;Parent=MS.gene57332 |
| chr4.1 | exon | 10644026 | 10644367 | 10644026 | ID=MS.gene57332.t1.exon1;Parent=MS.gene57332.t1 |
| chr4.1 | CDS | 10644026 | 10644367 | 10644026 | ID=cds.MS.gene57332.t1;Parent=MS.gene57332.t1 |
| chr4.1 | exon | 10643680 | 10643691 | 10643680 | ID=MS.gene57332.t1.exon2;Parent=MS.gene57332.t1 |
| chr4.1 | CDS | 10643680 | 10643691 | 10643680 | ID=cds.MS.gene57332.t1;Parent=MS.gene57332.t1 |
| Gene Sequence |
| Protein sequence |
